Rroxscaffold_4G00301740

threonine aspartase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0004496
Physical Location & Seq
Reverse (-)
22075467 .. 22080380
4914 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00301740.1

Sequence Viewer

Length: 1218 bp
ATGGCTGCTGCCGCTAAACAAGTACAACAGCAACTACTAGAAGATGAAAACGACAGATTCTTCGTCGCCGTTCATGTCGGCGCTGGATACCACGCGCCTTCCAACGAGAAGGCCCTCAGGTCCGCCATGAAACGCGCTTGCCTCGCCGCCGCTTCAATTCTCCGCCAGGGCTCTGGTAGGTGCATAGATGCTGTCACTGCAGCTATTCAGGTGTTGGAGGATGATCCAATCACGAATGCGGGCCGAGGGTCTAATTTGACCGAAGATGGTCGTGTAGAATGTGATGCCAGTATTATGGACGGGAATTCTGGAGTATTTGGAGCTGTTGGAGCATTACCAGGTGTCAAAAACCCCATCAAGGTTGCTGCTTTACTGGCCAAGGAGCAAATGATGGGATCCTCCCTGTTGGGCCGAATACCTCCCATCTTCCTCGTTGGTGAAGGTGCCCGCATATGGGCGAAGTCCAAGGGCATTGCTTTGTCGGCAAGCACATCAGCTGCTGATGAGTGGCTGGTAACAGAAAGGGCAAAAGCACATTGGAAAAAGTACAAAGCAATGGTAGATGATGCAAAAGCAAAGACAGAAACTTCTGCTGGTGGACTTCCCTGTAGTCCTCAAGAGGCTGCTGGAATTCCAGAGGAGGATTGCATCATGGACACTGTAGGAGTTATTTGTGTAGATGCTGACGGACAAATTGCATCTGGTGCCTCAAGTGGAGGCATTGCATTAAAGGTTGGTGGGCGTGTAGGGTTAGCAGCAATGCATGGTTCAGGCTGTTGGGCATCCTCCAAAGGTCTTTTTGGGGCTCCATGTATAATCGGTTGTTGTGTAAGTGGTGCTGGAGAATATCTGATGAAAGGATTTGCAGCTCGAGAGTGCTGTGTCTCTTTATCACTTTCACAGGCAGGTCCTGCCTCTGCTTGCATGAAAGTTTTACGCTCCTTTGTTCAAGAGAGTAATCAAAATTGTACTGATAGAAGTGCCGGATTTCTAGCTGTGCAAGCTGATGCTCCTGTGATGGTTTCAGGAAGTTCTCCAAAGTTAAAAGCTATTGAGATTGCAGCTGCTTACTCTTCCTTATCCTTTGGGATAGGATTTTTTGGAAGCTCTATGGAGCGACCCAAGGTTTCAATTCTCAGGAGTACAAAATTGCAAACCAAAACTGGAGTAGACCATTTTGAAGCACGAATTGATCTTTCTCCTAAAAGGAAGCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Asparaginase_2

Protein Analysis

405

Amino Acids

41.91

Weight (kDa)

8.33

Isoelectric Point (pI)

37.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asparaginase_2 PF01112 21 - 336 2.7e-48 Asparaginase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 896
AccB1I GGYRCC 2 cut(s) 443, 704
AccI GTMKAC 1 cut(s) 1170
AccII CGCG 2 cut(s) 95, 135
AciI CCGC 7 cut(s) 12, 123, 147, 150, 163, 239, 448
AclWI GGATC 3 cut(s) 218, 390, 403
AcoI YGGCCR 1 cut(s) 375
AcsI RAATTY 2 cut(s) 304, 630
AfaI GTAC 4 cut(s) 24, 548, 970, 1144
AfiI CCNNNNNNNGG 3 cut(s) 358, 453, 454
AgsI TTSAA 4 cut(s) 156, 950, 1131, 1181
AjnI CCWGG 2 cut(s) 165, 337
Alw26I GTCTC 1 cut(s) 889
AlwI GGATC 3 cut(s) 218, 390, 403
AlwNI CAGNNNCTG 2 cut(s) 500, 911
Ama87I CYCGRG 1 cut(s) 870
AoxI GGCC 4 cut(s) 111, 241, 375, 409
ApoI RAATTY 2 cut(s) 304, 630
ArsI GACNNNNNNTTYG 2 cut(s) 44, 76
AspLEI GCGC 3 cut(s) 83, 97, 137
AspS9I GGNCC 5 cut(s) 112, 120, 241, 409, 908
AsuHPI GGTGA 1 cut(s) 449
AvaI CYCGRG 1 cut(s) 870
AvaII GGWCC 2 cut(s) 120, 908
AxyI CCTNAGG 1 cut(s) 116
BaeGI GKGCMC 1 cut(s) 448
BalI TGGCCA 1 cut(s) 377
BamHI GGATCC 1 cut(s) 395
BanI GGYRCC 2 cut(s) 443, 704
BanII GRGCYC 2 cut(s) 173, 808
BbvI GCAGC 8 cut(s) 212, 352, 484, 610, 767, 878, 1051, 1073
BccI CCATC 5 cut(s) 260, 362, 385, 431, 1012
BceAI ACGGC 1 cut(s) 53
BciT130I CCWGG 2 cut(s) 167, 339
BciVI GTATCC 1 cut(s) 80
BcoDI GTCTC 1 cut(s) 889
BfaI CTAG 2 cut(s) 38, 992
BfmI CTRYAG 3 cut(s) 198, 607, 660
BfoI RGCGCY 1 cut(s) 84
BfuAI ACCTGC 1 cut(s) 896
BfuI GTATCC 1 cut(s) 80
Bme1390I CCNGG 2 cut(s) 167, 339
Bme18I GGWCC 2 cut(s) 120, 908
BmeT110I CYCGRG 1 cut(s) 870
BmgT120I GGNCC 5 cut(s) 112, 120, 241, 409, 908
BmiI GGNNCC 4 cut(s) 397, 445, 706, 807
BmrFI CCNGG 2 cut(s) 167, 339
BmsI GCATC 8 cut(s) 178, 274, 556, 657, 670, 707, 791, 997
BpmI CTGGAG 3 cut(s) 330, 861, 1185
BpuEI CTTGAG 2 cut(s) 600, 694
BsaJI CCNNGG 5 cut(s) 166, 244, 378, 465, 1122
Bsc4I CCNNNNNNNGG 3 cut(s) 358, 453, 454
Bse1I ACTGG 3 cut(s) 288, 378, 1168
Bse21I CCTNAGG 1 cut(s) 116
Bse3DI GCAATG 4 cut(s) 471, 561, 720, 765
BseBI CCWGG 2 cut(s) 167, 339
BseDI CCNNGG 5 cut(s) 166, 244, 378, 465, 1122
BseGI GGATG 2 cut(s) 226, 782
BseLI CCNNNNNNNGG 3 cut(s) 358, 453, 454
BseMI GCAATG 4 cut(s) 471, 561, 720, 765
BseMII CTCAG 2 cut(s) 130, 1150
BseNI ACTGG 3 cut(s) 288, 378, 1168
BseRI GAGGAG 1 cut(s) 653
BseSI GKGCMC 1 cut(s) 448
BseXI GCAGC 8 cut(s) 212, 352, 484, 610, 767, 878, 1051, 1073
Bsh1236I CGCG 2 cut(s) 95, 135
BshFI GGCC 4 cut(s) 113, 243, 377, 411
BshNI GGYRCC 2 cut(s) 443, 704
BsiHKCI CYCGRG 1 cut(s) 870
BsiSI CCGG 1 cut(s) 984
BslI CCNNNNNNNGG 3 cut(s) 358, 453, 454
BsmAI GTCTC 1 cut(s) 889
BsmI GAATGC 1 cut(s) 241
BsnI GGCC 4 cut(s) 113, 243, 377, 411
BsoBI CYCGRG 1 cut(s) 870
Bsp1286I GDGCHC 3 cut(s) 173, 448, 808
Bsp143I GATC 3 cut(s) 223, 395, 1192
BspACI CCGC 7 cut(s) 12, 123, 147, 150, 163, 239, 448
BspANI GGCC 4 cut(s) 113, 243, 377, 411
BspCNI CTCAG 2 cut(s) 129, 1149
BspFNI CGCG 2 cut(s) 95, 135
BspLI GGNNCC 4 cut(s) 397, 445, 706, 807
BspMAI CTGCAG 1 cut(s) 202
BspMI ACCTGC 1 cut(s) 896
BspPI GGATC 3 cut(s) 218, 390, 403
BspT107I GGYRCC 2 cut(s) 443, 704
BsrDI GCAATG 4 cut(s) 471, 561, 720, 765
BsrI ACTGG 3 cut(s) 288, 378, 1168
BssECI CCNNGG 5 cut(s) 166, 244, 378, 465, 1122
BssMI GATC 3 cut(s) 223, 395, 1192
BssT1I CCWWGG 3 cut(s) 378, 465, 1122
Bst2UI CCWGG 2 cut(s) 167, 339
Bst4CI ACNGT 1 cut(s) 661
Bst6I CTCTTC 1 cut(s) 1078
BstAPI GCANNNNNTGC 2 cut(s) 704, 911
BstC8I GCNNGC 6 cut(s) 139, 241, 448, 487, 922, 1002
BstDEI CTNAG 2 cut(s) 116, 1136
BstF5I GGATG 2 cut(s) 226, 782
BstFNI CGCG 2 cut(s) 95, 135
BstH2I RGCGCY 1 cut(s) 84
BstHHI GCGC 3 cut(s) 83, 97, 137
BstKTI GATC 3 cut(s) 226, 398, 1195
BstMAI GTCTC 1 cut(s) 889
BstMBI GATC 3 cut(s) 223, 395, 1192
BstMWI GCNNNNNNNGC 9 cut(s) 11, 143, 197, 329, 374, 482, 704, 911, 1001
BstNI CCWGG 2 cut(s) 167, 339
BstSCI CCNGG 2 cut(s) 165, 337
BstSFI CTRYAG 3 cut(s) 198, 607, 660
BstSLI GKGCMC 1 cut(s) 448
BstUI CGCG 2 cut(s) 95, 135
BstV1I GCAGC 8 cut(s) 212, 352, 484, 610, 767, 878, 1051, 1073
BstX2I RGATCY 1 cut(s) 395
BstXI CCANNNNNNTGG 2 cut(s) 173, 295
BstYI RGATCY 1 cut(s) 395
Bsu36I CCTNAGG 1 cut(s) 116
BsuI GTATCC 1 cut(s) 80
BsuRI GGCC 4 cut(s) 113, 243, 377, 411
BtsCI GGATG 2 cut(s) 226, 782
BtsI GCAGTG 1 cut(s) 195
BtsIMutI CAGTG 2 cut(s) 195, 657
BveI ACCTGC 1 cut(s) 896
Cac8I GCNNGC 6 cut(s) 139, 241, 448, 487, 922, 1002
CaiI CAGNNNCTG 2 cut(s) 500, 911
CfoI GCGC 3 cut(s) 83, 97, 137
Cfr13I GGNCC 5 cut(s) 112, 120, 241, 409, 908
CsiI ACCWGGT 1 cut(s) 337
Csp6I GTAC 4 cut(s) 23, 547, 969, 1143
CviAII CATG 6 cut(s) 74, 127, 652, 764, 810, 925
CviQI GTAC 4 cut(s) 23, 547, 969, 1143
DdeI CTNAG 2 cut(s) 116, 1136
DpnI GATC 3 cut(s) 225, 397, 1194
DpnII GATC 3 cut(s) 223, 395, 1192
EaeI YGGCCR 1 cut(s) 375
Eam1104I CTCTTC 1 cut(s) 1078
EarI CTCTTC 1 cut(s) 1078
EciI GGCGGA 2 cut(s) 112, 152
Eco130I CCWWGG 3 cut(s) 378, 465, 1122
Eco24I GRGCYC 2 cut(s) 173, 808
Eco47I GGWCC 2 cut(s) 120, 908
Eco81I CCTNAGG 1 cut(s) 116
Eco88I CYCGRG 1 cut(s) 870
EcoO109I RGGNCCY 2 cut(s) 112, 908
EcoRI GAATTC 2 cut(s) 304, 630
EcoRII CCWGG 2 cut(s) 165, 337
EcoT14I CCWWGG 3 cut(s) 378, 465, 1122
EcoT22I ATGCAT 1 cut(s) 765
EcoT38I GRGCYC 2 cut(s) 173, 808
ErhI CCWWGG 3 cut(s) 378, 465, 1122
FaeI CATG 6 cut(s) 77, 130, 655, 767, 813, 928
FatI CATG 6 cut(s) 73, 126, 651, 763, 809, 924
FauI CCCGC 2 cut(s) 232, 455
FauNDI CATATG 1 cut(s) 452
FblI GTMKAC 1 cut(s) 1170
FokI GGATG 2 cut(s) 233, 769
FriOI GRGCYC 2 cut(s) 173, 808
FspBI CTAG 2 cut(s) 38, 992
GlaI GCGC 3 cut(s) 82, 96, 136
GsuI CTGGAG 3 cut(s) 330, 861, 1185
HaeII RGCGCY 1 cut(s) 84
HaeIII GGCC 4 cut(s) 113, 243, 377, 411
HapII CCGG 1 cut(s) 984
HhaI GCGC 3 cut(s) 83, 97, 137
Hin1II CATG 6 cut(s) 77, 130, 655, 767, 813, 928
Hin6I GCGC 3 cut(s) 81, 95, 135
HinP1I GCGC 3 cut(s) 81, 95, 135
HinfI GANTC 1 cut(s) 57
HpaII CCGG 1 cut(s) 984
HphI GGTGA 1 cut(s) 449
Hpy166II GTNNAC 2 cut(s) 599, 1171
Hpy188I TCNGA 1 cut(s) 852
Hpy188III TCNNGA 8 cut(s) 232, 309, 617, 635, 872, 950, 1026, 1138
Hpy8I GTNNAC 2 cut(s) 599, 1171
Hpy99I CGWCG 1 cut(s) 68
HpyAV CCTTC 3 cut(s) 103, 108, 434
HpyCH4III ACNGT 1 cut(s) 661
HpyF10VI GCNNNNNNNGC 9 cut(s) 11, 143, 197, 329, 374, 482, 704, 911, 1001
HpyF3I CTNAG 2 cut(s) 116, 1136
Hsp92II CATG 6 cut(s) 77, 130, 655, 767, 813, 928
HspAI GCGC 3 cut(s) 81, 95, 135
Kzo9I GATC 3 cut(s) 223, 395, 1192
LmnI GCTCC 7 cut(s) 320, 329, 382, 811, 944, 1015, 1114
Lsp1109I GCAGC 8 cut(s) 212, 352, 484, 610, 767, 878, 1051, 1073
LweI GCATC 8 cut(s) 178, 274, 556, 657, 670, 707, 791, 997
MabI ACCWGGT 1 cut(s) 337
MaeI CTAG 2 cut(s) 38, 992
MaeIII GTNAC 2 cut(s) 193, 514
MalI GATC 3 cut(s) 225, 397, 1194
MboI GATC 3 cut(s) 223, 395, 1192
MboII GAAGA 5 cut(s) 52, 53, 275, 418, 1065
MflI RGATCY 1 cut(s) 395
MhlI GDGCHC 3 cut(s) 173, 448, 808
MlsI TGGCCA 1 cut(s) 377
MluCI AATT 9 cut(s) 156, 253, 304, 630, 693, 964, 1131, 1148, 1188
MluNI TGGCCA 1 cut(s) 377
MmeI TCCRAC 3 cut(s) 126, 195, 307
Mox20I TGGCCA 1 cut(s) 377
Mph1103I ATGCAT 1 cut(s) 765
MscI TGGCCA 1 cut(s) 377
MseI TTAA 2 cut(s) 728, 1043
Msp20I TGGCCA 1 cut(s) 377
MspA1I CMGCKG 2 cut(s) 497, 1064
MspI CCGG 1 cut(s) 984
MspR9I CCNGG 2 cut(s) 167, 339
Mva1269I GAATGC 1 cut(s) 241
MvaI CCWGG 2 cut(s) 167, 339
MvnI CGCG 2 cut(s) 95, 135
MwoI GCNNNNNNNGC 9 cut(s) 11, 143, 197, 329, 374, 482, 704, 911, 1001
NdeI CATATG 1 cut(s) 452
NdeII GATC 3 cut(s) 223, 395, 1192
NlaIII CATG 6 cut(s) 77, 130, 655, 767, 813, 928
NlaIV GGNNCC 4 cut(s) 397, 445, 706, 807
NmeAIII GCCGAG 1 cut(s) 269
NmuCI GTSAC 1 cut(s) 193
NsiI ATGCAT 1 cut(s) 765
PaeR7I CTCGAG 1 cut(s) 870
PctI GAATGC 1 cut(s) 241
PfeI GAWTC 1 cut(s) 57
PpuMI RGGWCCY 1 cut(s) 908
Psp5II RGGWCCY 1 cut(s) 908
Psp6I CCWGG 2 cut(s) 165, 337
PspGI CCWGG 2 cut(s) 165, 337
PspN4I GGNNCC 4 cut(s) 397, 445, 706, 807
PspPI GGNCC 5 cut(s) 112, 120, 241, 409, 908
PspPPI RGGWCCY 1 cut(s) 908
PstI CTGCAG 1 cut(s) 202
PstNI CAGNNNCTG 2 cut(s) 500, 911
PsuI RGATCY 1 cut(s) 395
PvuII CAGCTG 2 cut(s) 497, 1064
RsaI GTAC 4 cut(s) 24, 548, 970, 1144
RsaNI GTAC 4 cut(s) 23, 547, 969, 1143
SaqAI TTAA 2 cut(s) 728, 1043
Sau3AI GATC 3 cut(s) 223, 395, 1192
Sau96I GGNCC 5 cut(s) 112, 120, 241, 409, 908
ScrFI CCNGG 2 cut(s) 167, 339
SduI GDGCHC 3 cut(s) 173, 448, 808
SexAI ACCWGGT 1 cut(s) 337
SfaNI GCATC 8 cut(s) 178, 274, 556, 657, 670, 707, 791, 997
SfcI CTRYAG 3 cut(s) 198, 607, 660
Sfr274I CTCGAG 1 cut(s) 870
SinI GGWCC 2 cut(s) 120, 908
SlaI CTCGAG 1 cut(s) 870
SmlI CTYRAG 3 cut(s) 615, 709, 870
SmoI CTYRAG 3 cut(s) 615, 709, 870
Sse9I AATT 9 cut(s) 156, 253, 304, 630, 693, 964, 1131, 1148, 1188
SsiI CCGC 7 cut(s) 12, 123, 147, 150, 163, 239, 448
SspMI CTAG 2 cut(s) 38, 992
StyD4I CCNGG 2 cut(s) 165, 337
StyI CCWWGG 3 cut(s) 378, 465, 1122
TaaI ACNGT 1 cut(s) 661
TaqI TCGA 1 cut(s) 871
TaqII GACCGA 1 cut(s) 275
TasI AATT 9 cut(s) 156, 253, 304, 630, 693, 964, 1131, 1148, 1188
TatI WGTACW 4 cut(s) 22, 546, 968, 1142
TauI GCSGC 3 cut(s) 14, 149, 152
TfiI GAWTC 1 cut(s) 57
Tru1I TTAA 2 cut(s) 728, 1043
Tru9I TTAA 2 cut(s) 728, 1043
TscAI CASTG 2 cut(s) 202, 664
TseFI GTSAC 1 cut(s) 193
Tsp45I GTSAC 1 cut(s) 193
TspDTI ATGAA 5 cut(s) 60, 62, 143, 869, 941
TspGWI ACGGA 1 cut(s) 702
TspRI CASTG 2 cut(s) 202, 664
VpaK11BI GGWCC 2 cut(s) 120, 908
XapI RAATTY 2 cut(s) 304, 630
XhoI CTCGAG 1 cut(s) 870
XmiI GTMKAC 1 cut(s) 1170
XspI CTAG 2 cut(s) 38, 992
Zsp2I ATGCAT 1 cut(s) 765
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.