Rh1BG219400

threonine aspartase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0004496
Physical Location & Seq
Forward (+)
33913615 .. 33917457
3843 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG219400.1

Sequence Viewer

Length: 1263 bp
ATGGCTGCTGCGGCTAAACAAGTACAACAGCAACTACTAGAAGATGAAAACGACAGATTCTTCGTCGCCGTTCATGTCGGCGCTGGATACCACGCGCCTTCCAACGAGAAGGCCCTCAGGTCCGCCATGAAACGCGCTTGCCTCGCCGCCGCTTCAATTCTCCGCCAGGGCTCTGGTAGGTGCATAGATGCTGTCACTGCAGCTATTCAAGTGTTGGAGGATGATCCAATCACGAACGCGGGCCGGGGGTCTAATCTGACTGAAGATGGTCGTGTAGAATGTGATGCCAGTATTATGGACGGGAATTCTGGAGCATTTGGAGCTGTTGGAGCATTGTCAGGTGTCAAAAACCCCATCAAGGTTGCTGCTTTACTGGCCAAGGAGCAAATGATGGGATCCTCCCAGTTGGGCCGAATACCTCCCATCTTCCTCGTTGGTGAAGGTGCCCGCATATGGGCGAAGTCCAAGGGCATTGCTTTGTCGGCAAGCACATCAGCTGCTGATGAGTGGCTAGTGACAGAAAGGGCAAAAGCACATTGGAAAAAGTACAAAGCAATGGTAGATGATGCAAAAGTAAAGACAGAAACTTCTGCTGTTGGACTTCCTTGTAGTCCTCAAGAGGCTGCTGGAATTCCAGAATTAGAAGCTCAACCATGTGATTCTTTGAAGAATAATGGGGCAGAGGAGGATTGCATCATGGACACTGTTGGAGTTATTTGTGTCGATGCTGACGGACAAATTGCATCTGGTGCCTCAAGTGGGGGCATTGCATTAAAGGTTGGTGGGCGTGTAGGATTAGCAGCAATGCATGGTTCAGGATGTTGGGCATCCTCCAAAGGTCTTTTTGGGGCTCCATGTATAGTTGGCTGTTGTGTAAGTGGTGCTGGAGAATATCTGATGAAAGGATTTGCAGCTCGAGAGTGCTGTGTCTCTTTATCACTTTCACAGGCAGGTCCTGCCTCTGCTTGCATGAAAGTTTTACGCTCCTTTGTTCAAGAGAGTAATCAAAATTGTACTGATAGAAGTGCTGGATTTCTAGCTGTGCAAGCTGATGCTCCTGTGATGGTTTCAGGAAGTTCTCCAAAGTTAAAAGCTATTGAGATTGCAGCTGCTTACTCTTCCTTATCCTTTGGGATAGGATTTTTCGGAAGCTCTATGGAGCGACCCAAGGTTTCAATTCTCAGGAGTACAAAATTGCAAACCAAAACTGGAGTAGACCATTTTGAAGCACGAATTGATCTTTCTCCTAAAAGGAAGCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Asparaginase_2

Protein Analysis

420

Amino Acids

43.51

Weight (kDa)

7.85

Isoelectric Point (pI)

35.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asparaginase_2 PF01112 21 - 351 2.1e-48 Asparaginase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 941
AccB1I GGYRCC 2 cut(s) 443, 749
AccI GTMKAC 1 cut(s) 1215
AccII CGCG 3 cut(s) 95, 135, 239
AciI CCGC 7 cut(s) 11, 123, 147, 150, 163, 239, 448
AclWI GGATC 3 cut(s) 218, 390, 403
AcoI YGGCCR 1 cut(s) 375
AcsI RAATTY 2 cut(s) 304, 630
AcuI CTGAAG 1 cut(s) 282
AfaI GTAC 4 cut(s) 24, 548, 1015, 1189
AfiI CCNNNNNNNGG 4 cut(s) 358, 453, 454, 759
AgsI TTSAA 6 cut(s) 156, 209, 667, 995, 1176, 1226
AjnI CCWGG 1 cut(s) 165
Alw26I GTCTC 1 cut(s) 934
AlwI GGATC 3 cut(s) 218, 390, 403
AlwNI CAGNNNCTG 2 cut(s) 500, 956
Ama87I CYCGRG 1 cut(s) 915
AoxI GGCC 4 cut(s) 111, 241, 375, 409
ApoI RAATTY 2 cut(s) 304, 630
ArsI GACNNNNNNTTYG 2 cut(s) 44, 76
AspLEI GCGC 3 cut(s) 83, 97, 137
AspS9I GGNCC 5 cut(s) 112, 120, 241, 409, 953
AsuC2I CCSGG 1 cut(s) 245
AsuHPI GGTGA 1 cut(s) 449
AvaI CYCGRG 1 cut(s) 915
AvaII GGWCC 2 cut(s) 120, 953
AxyI CCTNAGG 1 cut(s) 116
BaeGI GKGCMC 1 cut(s) 448
BalI TGGCCA 1 cut(s) 377
BamHI GGATCC 1 cut(s) 395
BanI GGYRCC 2 cut(s) 443, 749
BanII GRGCYC 2 cut(s) 173, 853
BbvI GCAGC 8 cut(s) 212, 352, 484, 610, 812, 923, 1096, 1118
BccI CCATC 5 cut(s) 260, 362, 385, 431, 1057
BceAI ACGGC 1 cut(s) 53
BciT130I CCWGG 1 cut(s) 167
BciVI GTATCC 1 cut(s) 80
BcnI CCSGG 1 cut(s) 245
BcoDI GTCTC 1 cut(s) 934
BfaI CTAG 3 cut(s) 38, 512, 1037
BfmI CTRYAG 1 cut(s) 198
BfoI RGCGCY 1 cut(s) 84
BfuAI ACCTGC 1 cut(s) 941
BfuI GTATCC 1 cut(s) 80
Bme1390I CCNGG 2 cut(s) 167, 245
Bme18I GGWCC 2 cut(s) 120, 953
BmeT110I CYCGRG 1 cut(s) 915
BmgT120I GGNCC 5 cut(s) 112, 120, 241, 409, 953
BmiI GGNNCC 4 cut(s) 397, 445, 751, 852
BmrFI CCNGG 2 cut(s) 167, 245
BmrI ACTGGG 1 cut(s) 397
BmsI GCATC 8 cut(s) 178, 274, 556, 702, 715, 752, 836, 1042
BmuI ACTGGG 1 cut(s) 397
BpmI CTGGAG 3 cut(s) 330, 906, 1230
BpuEI CTTGAG 2 cut(s) 600, 739
BpuMI CCSGG 1 cut(s) 245
BsaJI CCNNGG 5 cut(s) 166, 244, 378, 465, 1167
Bsc4I CCNNNNNNNGG 4 cut(s) 358, 453, 454, 759
Bse1I ACTGG 4 cut(s) 288, 378, 403, 1213
Bse21I CCTNAGG 1 cut(s) 116
Bse3DI GCAATG 4 cut(s) 471, 561, 765, 810
BseBI CCWGG 1 cut(s) 167
BseDI CCNNGG 5 cut(s) 166, 244, 378, 465, 1167
BseGI GGATG 3 cut(s) 226, 824, 827
BseLI CCNNNNNNNGG 4 cut(s) 358, 453, 454, 759
BseMI GCAATG 4 cut(s) 471, 561, 765, 810
BseMII CTCAG 2 cut(s) 130, 1195
BseNI ACTGG 4 cut(s) 288, 378, 403, 1213
BseRI GAGGAG 1 cut(s) 698
BseSI GKGCMC 1 cut(s) 448
BseXI GCAGC 8 cut(s) 212, 352, 484, 610, 812, 923, 1096, 1118
Bsh1236I CGCG 3 cut(s) 95, 135, 239
BshFI GGCC 4 cut(s) 113, 243, 377, 411
BshNI GGYRCC 2 cut(s) 443, 749
BsiHKCI CYCGRG 1 cut(s) 915
BsiSI CCGG 1 cut(s) 244
BslI CCNNNNNNNGG 4 cut(s) 358, 453, 454, 759
BsmAI GTCTC 1 cut(s) 934
BsnI GGCC 4 cut(s) 113, 243, 377, 411
BsoBI CYCGRG 1 cut(s) 915
Bsp1286I GDGCHC 3 cut(s) 173, 448, 853
Bsp143I GATC 3 cut(s) 223, 395, 1237
BspACI CCGC 7 cut(s) 11, 123, 147, 150, 163, 239, 448
BspANI GGCC 4 cut(s) 113, 243, 377, 411
BspCNI CTCAG 2 cut(s) 129, 1194
BspFNI CGCG 3 cut(s) 95, 135, 239
BspLI GGNNCC 4 cut(s) 397, 445, 751, 852
BspMAI CTGCAG 1 cut(s) 202
BspMI ACCTGC 1 cut(s) 941
BspPI GGATC 3 cut(s) 218, 390, 403
BspT107I GGYRCC 2 cut(s) 443, 749
BsrDI GCAATG 4 cut(s) 471, 561, 765, 810
BsrI ACTGG 4 cut(s) 288, 378, 403, 1213
BssECI CCNNGG 5 cut(s) 166, 244, 378, 465, 1167
BssMI GATC 3 cut(s) 223, 395, 1237
BssT1I CCWWGG 3 cut(s) 378, 465, 1167
Bst2UI CCWGG 1 cut(s) 167
Bst4CI ACNGT 1 cut(s) 706
Bst6I CTCTTC 1 cut(s) 1123
BstAPI GCANNNNNTGC 2 cut(s) 749, 956
BstC8I GCNNGC 6 cut(s) 139, 241, 448, 487, 967, 1047
BstDEI CTNAG 2 cut(s) 116, 1181
BstF5I GGATG 3 cut(s) 226, 824, 827
BstFNI CGCG 3 cut(s) 95, 135, 239
BstH2I RGCGCY 1 cut(s) 84
BstHHI GCGC 3 cut(s) 83, 97, 137
BstKTI GATC 3 cut(s) 226, 398, 1240
BstMAI GTCTC 1 cut(s) 934
BstMBI GATC 3 cut(s) 223, 395, 1237
BstNI CCWGG 1 cut(s) 167
BstSCI CCNGG 2 cut(s) 165, 243
BstSFI CTRYAG 1 cut(s) 198
BstSLI GKGCMC 1 cut(s) 448
BstUI CGCG 3 cut(s) 95, 135, 239
BstV1I GCAGC 8 cut(s) 212, 352, 484, 610, 812, 923, 1096, 1118
BstX2I RGATCY 1 cut(s) 395
BstXI CCANNNNNNTGG 2 cut(s) 173, 295
BstYI RGATCY 1 cut(s) 395
Bsu36I CCTNAGG 1 cut(s) 116
BsuI GTATCC 1 cut(s) 80
BsuRI GGCC 4 cut(s) 113, 243, 377, 411
BtsCI GGATG 3 cut(s) 226, 824, 827
BtsI GCAGTG 1 cut(s) 195
BtsIMutI CAGTG 2 cut(s) 195, 702
BveI ACCTGC 1 cut(s) 941
Cac8I GCNNGC 6 cut(s) 139, 241, 448, 487, 967, 1047
CaiI CAGNNNCTG 2 cut(s) 500, 956
CfoI GCGC 3 cut(s) 83, 97, 137
Cfr13I GGNCC 5 cut(s) 112, 120, 241, 409, 953
Csp6I GTAC 4 cut(s) 23, 547, 1014, 1188
CviAII CATG 7 cut(s) 74, 127, 654, 697, 809, 855, 970
CviQI GTAC 4 cut(s) 23, 547, 1014, 1188
DdeI CTNAG 2 cut(s) 116, 1181
DpnI GATC 3 cut(s) 225, 397, 1239
DpnII GATC 3 cut(s) 223, 395, 1237
EaeI YGGCCR 1 cut(s) 375
Eam1104I CTCTTC 1 cut(s) 1123
EarI CTCTTC 1 cut(s) 1123
EciI GGCGGA 2 cut(s) 112, 152
Eco130I CCWWGG 3 cut(s) 378, 465, 1167
Eco24I GRGCYC 2 cut(s) 173, 853
Eco47I GGWCC 2 cut(s) 120, 953
Eco57I CTGAAG 1 cut(s) 282
Eco81I CCTNAGG 1 cut(s) 116
Eco88I CYCGRG 1 cut(s) 915
EcoO109I RGGNCCY 2 cut(s) 112, 953
EcoRI GAATTC 2 cut(s) 304, 630
EcoRII CCWGG 1 cut(s) 165
EcoT14I CCWWGG 3 cut(s) 378, 465, 1167
EcoT22I ATGCAT 1 cut(s) 810
EcoT38I GRGCYC 2 cut(s) 173, 853
ErhI CCWWGG 3 cut(s) 378, 465, 1167
FaeI CATG 7 cut(s) 77, 130, 657, 700, 812, 858, 973
FatI CATG 7 cut(s) 73, 126, 653, 696, 808, 854, 969
FauI CCCGC 2 cut(s) 232, 455
FauNDI CATATG 1 cut(s) 452
FblI GTMKAC 1 cut(s) 1215
FokI GGATG 3 cut(s) 233, 814, 831
FriOI GRGCYC 2 cut(s) 173, 853
FspBI CTAG 3 cut(s) 38, 512, 1037
GlaI GCGC 3 cut(s) 82, 96, 136
GsuI CTGGAG 3 cut(s) 330, 906, 1230
HaeII RGCGCY 1 cut(s) 84
HaeIII GGCC 4 cut(s) 113, 243, 377, 411
HapII CCGG 1 cut(s) 244
HhaI GCGC 3 cut(s) 83, 97, 137
Hin1II CATG 7 cut(s) 77, 130, 657, 700, 812, 858, 973
Hin6I GCGC 3 cut(s) 81, 95, 135
HinP1I GCGC 3 cut(s) 81, 95, 135
HinfI GANTC 2 cut(s) 57, 659
HpaII CCGG 1 cut(s) 244
HphI GGTGA 1 cut(s) 449
Hpy166II GTNNAC 1 cut(s) 1216
Hpy188I TCNGA 3 cut(s) 258, 897, 1148
Hpy188III TCNNGA 9 cut(s) 232, 309, 617, 635, 816, 917, 995, 1071, 1183
Hpy8I GTNNAC 1 cut(s) 1216
Hpy99I CGWCG 1 cut(s) 68
HpyAV CCTTC 3 cut(s) 103, 108, 434
HpyCH4III ACNGT 1 cut(s) 706
HpyF3I CTNAG 2 cut(s) 116, 1181
Hsp92II CATG 7 cut(s) 77, 130, 657, 700, 812, 858, 973
HspAI GCGC 3 cut(s) 81, 95, 135
Kzo9I GATC 3 cut(s) 223, 395, 1237
LmnI GCTCC 8 cut(s) 311, 320, 329, 382, 856, 989, 1060, 1159
Lsp1109I GCAGC 8 cut(s) 212, 352, 484, 610, 812, 923, 1096, 1118
LweI GCATC 8 cut(s) 178, 274, 556, 702, 715, 752, 836, 1042
MaeI CTAG 3 cut(s) 38, 512, 1037
MaeIII GTNAC 2 cut(s) 193, 514
MalI GATC 3 cut(s) 225, 397, 1239
MboI GATC 3 cut(s) 223, 395, 1237
MboII GAAGA 6 cut(s) 52, 53, 275, 418, 679, 1110
MflI RGATCY 1 cut(s) 395
MhlI GDGCHC 3 cut(s) 173, 448, 853
MlsI TGGCCA 1 cut(s) 377
MluCI AATT 9 cut(s) 156, 304, 630, 638, 738, 1009, 1176, 1193, 1233
MluNI TGGCCA 1 cut(s) 377
MmeI TCCRAC 5 cut(s) 126, 195, 307, 577, 688
Mox20I TGGCCA 1 cut(s) 377
Mph1103I ATGCAT 1 cut(s) 810
MscI TGGCCA 1 cut(s) 377
MseI TTAA 2 cut(s) 773, 1088
Msp20I TGGCCA 1 cut(s) 377
MspA1I CMGCKG 2 cut(s) 497, 1109
MspI CCGG 1 cut(s) 244
MspR9I CCNGG 2 cut(s) 167, 245
MvaI CCWGG 1 cut(s) 167
MvnI CGCG 3 cut(s) 95, 135, 239
NciI CCSGG 1 cut(s) 245
NdeI CATATG 1 cut(s) 452
NdeII GATC 3 cut(s) 223, 395, 1237
NlaIII CATG 7 cut(s) 77, 130, 657, 700, 812, 858, 973
NlaIV GGNNCC 4 cut(s) 397, 445, 751, 852
NmuCI GTSAC 2 cut(s) 193, 514
NsiI ATGCAT 1 cut(s) 810
PaeR7I CTCGAG 1 cut(s) 915
PfeI GAWTC 2 cut(s) 57, 659
PpuMI RGGWCCY 1 cut(s) 953
Psp5II RGGWCCY 1 cut(s) 953
Psp6I CCWGG 1 cut(s) 165
PspGI CCWGG 1 cut(s) 165
PspN4I GGNNCC 4 cut(s) 397, 445, 751, 852
PspPI GGNCC 5 cut(s) 112, 120, 241, 409, 953
PspPPI RGGWCCY 1 cut(s) 953
PstI CTGCAG 1 cut(s) 202
PstNI CAGNNNCTG 2 cut(s) 500, 956
PsuI RGATCY 1 cut(s) 395
PvuII CAGCTG 2 cut(s) 497, 1109
RsaI GTAC 4 cut(s) 24, 548, 1015, 1189
RsaNI GTAC 4 cut(s) 23, 547, 1014, 1188
SaqAI TTAA 2 cut(s) 773, 1088
Sau3AI GATC 3 cut(s) 223, 395, 1237
Sau96I GGNCC 5 cut(s) 112, 120, 241, 409, 953
ScrFI CCNGG 2 cut(s) 167, 245
SduI GDGCHC 3 cut(s) 173, 448, 853
SfaNI GCATC 8 cut(s) 178, 274, 556, 702, 715, 752, 836, 1042
SfcI CTRYAG 1 cut(s) 198
Sfr274I CTCGAG 1 cut(s) 915
SinI GGWCC 2 cut(s) 120, 953
SlaI CTCGAG 1 cut(s) 915
SmlI CTYRAG 3 cut(s) 615, 754, 915
SmoI CTYRAG 3 cut(s) 615, 754, 915
Sse9I AATT 9 cut(s) 156, 304, 630, 638, 738, 1009, 1176, 1193, 1233
SsiI CCGC 7 cut(s) 11, 123, 147, 150, 163, 239, 448
SspMI CTAG 3 cut(s) 38, 512, 1037
StyD4I CCNGG 2 cut(s) 165, 243
StyI CCWWGG 3 cut(s) 378, 465, 1167
TaaI ACNGT 1 cut(s) 706
TaqI TCGA 2 cut(s) 723, 916
TasI AATT 9 cut(s) 156, 304, 630, 638, 738, 1009, 1176, 1193, 1233
TatI WGTACW 4 cut(s) 22, 546, 1013, 1187
TauI GCSGC 3 cut(s) 14, 149, 152
TfiI GAWTC 2 cut(s) 57, 659
Tru1I TTAA 2 cut(s) 773, 1088
Tru9I TTAA 2 cut(s) 773, 1088
TscAI CASTG 2 cut(s) 202, 709
TseFI GTSAC 2 cut(s) 193, 514
Tsp45I GTSAC 2 cut(s) 193, 514
TspDTI ATGAA 5 cut(s) 60, 62, 143, 914, 986
TspGWI ACGGA 1 cut(s) 747
TspRI CASTG 2 cut(s) 202, 709
VpaK11BI GGWCC 2 cut(s) 120, 953
XapI RAATTY 2 cut(s) 304, 630
XhoI CTCGAG 1 cut(s) 915
XmiI GTMKAC 1 cut(s) 1215
XspI CTAG 3 cut(s) 38, 512, 1037
Zsp2I ATGCAT 1 cut(s) 810
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.