MD07G1141600.v1.1

threonine aspartase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0004496
Physical Location & Seq
Reverse (-)
20524426 .. 20528040
3615 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1141600.v1.1.491

Sequence Viewer

Length: 1251 bp
ATGGCTGCCGCGAACCAAATGCAAGAAGAAGACGAAAACGCCACGTTCTTCGTGGCGGTTCACGTCGGCGCTGGATACCACGCGCCATCTAACGAGAAAGCCCTCAAGTCCGCCGTCAAACGCGCTTGTCTAGCCGCCGGTTCAATCCTCCGCAAGGGTTCTGGTAGATGCATAGATGCTGTTACTGCAGCTATTCAGGTTTTAGAGGATGATCCAAGCACGAATGCAGGCAGAGGCTCCAATTTGACTGAAGATGGTCGTGTAGAATGCGATGCCAGTATGATGGACGGAAATTCTGGAGTATTTGGAGCTGTTGGAGCAATTCCAGGTGTCAGAAACCCCATCCAGGTCGCTGCTTTATTGGCCAAGGAGCAAATGATGGGATCCCCTCTGTTGGGCCGAATACCTCCCACCTTCCTGGTTGGTGAAGGTGCCCGTGCATGGGCAAAGTCCAAGGGCCTTGTTTTGGCAGCAAGTACCAGAGCTGATGATGAGTGGCTGGTGACAGAAAGAGCAAAATCACAATGGAGAAAGTACAAAGCAATGGTTGATGATGCAAAAGCAAAGACAGAAAATTCTGATTCTAGACTTTCTTGTAGTCCTCAAGAGACTGCTGGAATGTCAGAATCAGAAGCTCAACCATGCGATTATTTGAAGCATAATGGAGCTGAAGAGGATTGCATCATGGATACTGTTGGAGTTATTTGTGTGGATGCTGAAGGACAAATAGCATCTGGCGCCTCAAGTGGGGGAATTGCACTGAAGGTTGGTGGGCGTGTGGGATTAGCAGCTATGCACGGTGCAGGCTGTTGGGCATCCTCCAAAGGTGTTTTTGGGGCTCCATGTATAGTTGGCTGTTGTGTAAGTGGTGCTGGAGAATATCTAATGAAAGGATTTGCAGCTCGTGAGTCCTGCGTCTCTTTGTCAGTTTCACAGGCAGGCCCTGCCTCTGCTTGCATGAAAGTTTTACGTTCTGTTGTTCAAGAGAGTAATAACAATTGTACTGATAGAAGTGCCGGAATTCTACTTGTGCAAGCTGATGCTCCTATACTGGTTGCAGGAAGTTCTCCAAAGTTGAAAGCTGTAGAGATTGCAGCTGCTTACACGTCTTTATCCTTTGGAATAGGATATTTTGGGAGCGCTATGGAGCGGCCCAAGGTTTCAATTCTTAGGAGTACAAAATTGCAAAGCAAAACTGGAGTGGACCATTTTGAAGCACGAATTGATCTTTCGGGTGGAAAGCTGTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Asparaginase_2

Protein Analysis

417

Amino Acids

42.99

Weight (kDa)

6.27

Isoelectric Point (pI)

37.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asparaginase_2 PF01112 17 - 331 3.3e-48 Asparaginase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 431, 737
AccBSI CCGCTC 1 cut(s) 1150
AccII CGCG 3 cut(s) 11, 83, 123
AciI CCGC 6 cut(s) 9, 56, 111, 135, 151, 1150
AclWI GGATC 3 cut(s) 206, 378, 391
AcoI YGGCCR 1 cut(s) 363
AcsI RAATTY 3 cut(s) 292, 574, 1020
AcuI CTGAAG 4 cut(s) 270, 690, 738, 782
AcyI GRCGYC 1 cut(s) 738
AfaI GTAC 4 cut(s) 478, 536, 1003, 1177
AfeI AGCGCT 1 cut(s) 1141
AfiI CCNNNNNNNGG 8 cut(s) 154, 346, 394, 395, 441, 442, 466, 747
AflIII ACRYGT 1 cut(s) 1104
AgsI TTSAA 6 cut(s) 144, 655, 983, 1078, 1164, 1214
AjiI CACGTC 2 cut(s) 64, 1107
AjnI CCWGG 3 cut(s) 325, 345, 417
Alw26I GTCTC 2 cut(s) 602, 922
AlwI GGATC 3 cut(s) 206, 378, 391
AlwNI CAGNNNCTG 1 cut(s) 944
Aor51HI AGCGCT 1 cut(s) 1141
AoxI GGCC 5 cut(s) 363, 397, 457, 940, 1151
ApeKI GCWGC 8 cut(s) 5, 188, 353, 470, 788, 899, 1094, 1097
ApoI RAATTY 3 cut(s) 292, 574, 1020
AspLEI GCGC 5 cut(s) 71, 85, 125, 740, 1142
AspS9I GGNCC 5 cut(s) 397, 457, 941, 1152, 1204
AsuHPI GGTGA 2 cut(s) 437, 514
AvaII GGWCC 1 cut(s) 1204
BaeGI GKGCMC 1 cut(s) 436
BalI TGGCCA 1 cut(s) 365
BamHI GGATCC 1 cut(s) 383
BanI GGYRCC 2 cut(s) 431, 737
BanII GRGCYC 1 cut(s) 841
BauI CACGAG 1 cut(s) 903
BbsI GAAGAC 1 cut(s) 36
BbvI GCAGC 7 cut(s) 200, 340, 482, 800, 911, 1084, 1106
BccI CCATC 5 cut(s) 94, 248, 277, 350, 373
BceAI ACGGC 1 cut(s) 98
BcgI CGANNNNNNTGC 1 cut(s) 35
BciT130I CCWGG 3 cut(s) 327, 347, 419
BciVI GTATCC 2 cut(s) 68, 682
BcoDI GTCTC 2 cut(s) 602, 922
BfaI CTAG 2 cut(s) 131, 585
BfmI CTRYAG 2 cut(s) 186, 1083
BfoI RGCGCY 3 cut(s) 72, 741, 1143
BfuI GTATCC 2 cut(s) 68, 682
Bme1390I CCNGG 3 cut(s) 327, 347, 419
Bme18I GGWCC 1 cut(s) 1204
BmgBI CACGTC 2 cut(s) 64, 1107
BmgT120I GGNCC 5 cut(s) 397, 457, 941, 1152, 1204
BmiI GGNNCC 5 cut(s) 238, 385, 433, 739, 840
BmrFI CCNGG 3 cut(s) 327, 347, 419
BmsI GCATC 9 cut(s) 158, 166, 262, 544, 690, 703, 740, 824, 1030
BpiI GAAGAC 1 cut(s) 36
BpmI CTGGAG 3 cut(s) 318, 894, 1218
BpuEI CTTGAG 3 cut(s) 89, 588, 727
BsaHI GRCGYC 1 cut(s) 738
BsaJI CCNNGG 3 cut(s) 366, 453, 1155
Bsc4I CCNNNNNNNGG 8 cut(s) 154, 346, 394, 395, 441, 442, 466, 747
Bse118I RCCGGY 1 cut(s) 137
Bse1I ACTGG 3 cut(s) 276, 1056, 1201
Bse3DI GCAATG 1 cut(s) 549
BseBI CCWGG 3 cut(s) 327, 347, 419
BseDI CCNNGG 3 cut(s) 366, 453, 1155
BseGI GGATG 4 cut(s) 214, 342, 718, 815
BseLI CCNNNNNNNGG 8 cut(s) 154, 346, 394, 395, 441, 442, 466, 747
BseMI GCAATG 1 cut(s) 549
BseNI ACTGG 3 cut(s) 276, 1056, 1201
BseSI GKGCMC 1 cut(s) 436
BseXI GCAGC 7 cut(s) 200, 340, 482, 800, 911, 1084, 1106
BsgI GTGCAG 1 cut(s) 822
Bsh1236I CGCG 3 cut(s) 11, 83, 123
BshFI GGCC 5 cut(s) 365, 399, 459, 942, 1153
BshNI GGYRCC 2 cut(s) 431, 737
BsiSI CCGG 2 cut(s) 138, 1017
BslI CCNNNNNNNGG 8 cut(s) 154, 346, 394, 395, 441, 442, 466, 747
BsmAI GTCTC 2 cut(s) 602, 922
BsmBI CGTCTC 1 cut(s) 922
BsmI GAATGC 2 cut(s) 229, 272
BsnI GGCC 5 cut(s) 365, 399, 459, 942, 1153
Bsp1286I GDGCHC 2 cut(s) 436, 841
Bsp143I GATC 3 cut(s) 211, 383, 1225
BspACI CCGC 6 cut(s) 9, 56, 111, 135, 151, 1150
BspANI GGCC 5 cut(s) 365, 399, 459, 942, 1153
BspFNI CGCG 3 cut(s) 11, 83, 123
BspLI GGNNCC 5 cut(s) 238, 385, 433, 739, 840
BspMAI CTGCAG 1 cut(s) 190
BspPI GGATC 3 cut(s) 206, 378, 391
BspT107I GGYRCC 2 cut(s) 431, 737
BsrBI CCGCTC 1 cut(s) 1150
BsrDI GCAATG 1 cut(s) 549
BsrFI RCCGGY 1 cut(s) 137
BsrI ACTGG 3 cut(s) 276, 1056, 1201
BssAI RCCGGY 1 cut(s) 137
BssECI CCNNGG 3 cut(s) 366, 453, 1155
BssMI GATC 3 cut(s) 211, 383, 1225
BssNI GRCGYC 1 cut(s) 738
BssSI CACGAG 1 cut(s) 903
BssT1I CCWWGG 3 cut(s) 366, 453, 1155
Bst2BI CACGAG 1 cut(s) 903
Bst2UI CCWGG 3 cut(s) 327, 347, 419
Bst4CI ACNGT 2 cut(s) 694, 800
Bst6I CTCTTC 1 cut(s) 666
BstACI GRCGYC 1 cut(s) 738
BstAPI GCANNNNNTGC 1 cut(s) 944
BstC8I GCNNGC 5 cut(s) 229, 805, 940, 955, 1035
BstDEI CTNAG 1 cut(s) 1169
BstENI CCTNNNNNAGG 1 cut(s) 152
BstF5I GGATG 4 cut(s) 214, 342, 718, 815
BstFNI CGCG 3 cut(s) 11, 83, 123
BstH2I RGCGCY 3 cut(s) 72, 741, 1143
BstHHI GCGC 5 cut(s) 71, 85, 125, 740, 1142
BstKTI GATC 3 cut(s) 214, 386, 1228
BstMAI GTCTC 2 cut(s) 602, 922
BstMBI GATC 3 cut(s) 211, 383, 1225
BstMWI GCNNNNNNNGC 6 cut(s) 131, 185, 317, 362, 737, 944
BstNI CCWGG 3 cut(s) 327, 347, 419
BstSCI CCNGG 3 cut(s) 325, 345, 417
BstSFI CTRYAG 2 cut(s) 186, 1083
BstSLI GKGCMC 1 cut(s) 436
BstUI CGCG 3 cut(s) 11, 83, 123
BstV1I GCAGC 7 cut(s) 200, 340, 482, 800, 911, 1084, 1106
BstV2I GAAGAC 1 cut(s) 36
BstX2I RGATCY 1 cut(s) 383
BstXI CCANNNNNNTGG 2 cut(s) 283, 418
BstYI RGATCY 1 cut(s) 383
BsuI GTATCC 2 cut(s) 68, 682
BsuRI GGCC 5 cut(s) 365, 399, 459, 942, 1153
BtgZI GCGATG 1 cut(s) 285
BtrI CACGTC 2 cut(s) 64, 1107
BtsCI GGATG 4 cut(s) 214, 342, 718, 815
BtsIMutI CAGTG 1 cut(s) 758
Cac8I GCNNGC 5 cut(s) 229, 805, 940, 955, 1035
CaiI CAGNNNCTG 1 cut(s) 944
CfoI GCGC 5 cut(s) 71, 85, 125, 740, 1142
Cfr10I RCCGGY 1 cut(s) 137
Cfr13I GGNCC 5 cut(s) 397, 457, 941, 1152, 1204
CseI GACGC 1 cut(s) 904
Csp6I GTAC 4 cut(s) 477, 535, 1002, 1176
CviAII CATG 5 cut(s) 441, 642, 685, 843, 958
CviQI GTAC 4 cut(s) 477, 535, 1002, 1176
DdeI CTNAG 1 cut(s) 1169
DinI GGCGCC 1 cut(s) 739
DpnI GATC 3 cut(s) 213, 385, 1227
DpnII GATC 3 cut(s) 211, 383, 1225
EaeI YGGCCR 1 cut(s) 363
Eam1104I CTCTTC 1 cut(s) 666
EarI CTCTTC 1 cut(s) 666
EciI GGCGGA 1 cut(s) 100
Eco130I CCWWGG 3 cut(s) 366, 453, 1155
Eco24I GRGCYC 1 cut(s) 841
Eco47I GGWCC 1 cut(s) 1204
Eco47III AGCGCT 1 cut(s) 1141
Eco57I CTGAAG 4 cut(s) 270, 690, 738, 782
EcoNI CCTNNNNNAGG 1 cut(s) 152
EcoO109I RGGNCCY 2 cut(s) 457, 941
EcoRI GAATTC 1 cut(s) 1020
EcoRII CCWGG 3 cut(s) 325, 345, 417
EcoT14I CCWWGG 3 cut(s) 366, 453, 1155
EcoT22I ATGCAT 1 cut(s) 173
EcoT38I GRGCYC 1 cut(s) 841
EgeI GGCGCC 1 cut(s) 739
EheI GGCGCC 1 cut(s) 739
ErhI CCWWGG 3 cut(s) 366, 453, 1155
Esp3I CGTCTC 1 cut(s) 922
FaeI CATG 5 cut(s) 444, 645, 688, 846, 961
FatI CATG 5 cut(s) 440, 641, 684, 842, 957
FokI GGATG 4 cut(s) 221, 329, 725, 802
FriOI GRGCYC 1 cut(s) 841
FspBI CTAG 2 cut(s) 131, 585
GlaI GCGC 5 cut(s) 70, 84, 124, 739, 1141
GsuI CTGGAG 3 cut(s) 318, 894, 1218
HaeII RGCGCY 3 cut(s) 72, 741, 1143
HaeIII GGCC 5 cut(s) 365, 399, 459, 942, 1153
HapII CCGG 2 cut(s) 138, 1017
HgaI GACGC 1 cut(s) 904
HhaI GCGC 5 cut(s) 71, 85, 125, 740, 1142
Hin1I GRCGYC 1 cut(s) 738
Hin1II CATG 5 cut(s) 444, 645, 688, 846, 961
Hin6I GCGC 5 cut(s) 69, 83, 123, 738, 1140
HinP1I GCGC 5 cut(s) 69, 83, 123, 738, 1140
HinfI GANTC 3 cut(s) 581, 626, 908
HpaII CCGG 2 cut(s) 138, 1017
HphI GGTGA 2 cut(s) 437, 514
Hpy166II GTNNAC 2 cut(s) 61, 1204
Hpy188I TCNGA 4 cut(s) 335, 580, 625, 631
Hpy188III TCNNGA 6 cut(s) 297, 585, 605, 905, 983, 1248
Hpy8I GTNNAC 2 cut(s) 61, 1204
Hpy99I CGWCG 1 cut(s) 68
HpyAV CCTTC 4 cut(s) 422, 424, 713, 757
HpyCH4III ACNGT 2 cut(s) 694, 800
HpyCH4IV ACGT 4 cut(s) 44, 63, 970, 1106
HpyF10VI GCNNNNNNNGC 6 cut(s) 131, 185, 317, 362, 737, 944
HpyF3I CTNAG 1 cut(s) 1169
HpySE526I ACGT 4 cut(s) 44, 63, 970, 1106
Hsp92I GRCGYC 1 cut(s) 738
Hsp92II CATG 5 cut(s) 444, 645, 688, 846, 961
HspAI GCGC 5 cut(s) 69, 83, 123, 738, 1140
KasI GGCGCC 1 cut(s) 737
Kzo9I GATC 3 cut(s) 211, 383, 1225
LmnI GCTCC 9 cut(s) 242, 308, 317, 370, 665, 844, 1048, 1137, 1147
Lsp1109I GCAGC 7 cut(s) 200, 340, 482, 800, 911, 1084, 1106
LweI GCATC 9 cut(s) 158, 166, 262, 544, 690, 703, 740, 824, 1030
MaeI CTAG 2 cut(s) 131, 585
MaeII ACGT 4 cut(s) 44, 63, 970, 1106
MaeIII GTNAC 2 cut(s) 181, 502
MalI GATC 3 cut(s) 213, 385, 1227
MbiI CCGCTC 1 cut(s) 1150
MboI GATC 3 cut(s) 211, 383, 1225
MboII GAAGA 5 cut(s) 38, 40, 41, 263, 683
MfeI CAATTG 1 cut(s) 997
MflI RGATCY 1 cut(s) 383
MhlI GDGCHC 2 cut(s) 436, 841
MlsI TGGCCA 1 cut(s) 365
MluNI TGGCCA 1 cut(s) 365
Mly113I GGCGCC 1 cut(s) 738
MlyI GAGTC 1 cut(s) 917
MmeI TCCRAC 2 cut(s) 295, 676
Mox20I TGGCCA 1 cut(s) 365
Mph1103I ATGCAT 1 cut(s) 173
MscI TGGCCA 1 cut(s) 365
Msp20I TGGCCA 1 cut(s) 365
MspA1I CMGCKG 1 cut(s) 1097
MspI CCGG 2 cut(s) 138, 1017
MspR9I CCNGG 3 cut(s) 327, 347, 419
MunI CAATTG 1 cut(s) 997
Mva1269I GAATGC 2 cut(s) 229, 272
MvaI CCWGG 3 cut(s) 327, 347, 419
MvnI CGCG 3 cut(s) 11, 83, 123
MwoI GCNNNNNNNGC 6 cut(s) 131, 185, 317, 362, 737, 944
NarI GGCGCC 1 cut(s) 738
NdeII GATC 3 cut(s) 211, 383, 1225
NlaIII CATG 5 cut(s) 444, 645, 688, 846, 961
NlaIV GGNNCC 5 cut(s) 238, 385, 433, 739, 840
NmuCI GTSAC 1 cut(s) 502
NsiI ATGCAT 1 cut(s) 173
PctI GAATGC 2 cut(s) 229, 272
PfeI GAWTC 2 cut(s) 581, 626
PleI GAGTC 1 cut(s) 916
PluTI GGCGCC 1 cut(s) 741
PpsI GAGTC 1 cut(s) 916
Psp6I CCWGG 3 cut(s) 325, 345, 417
PspGI CCWGG 3 cut(s) 325, 345, 417
PspN4I GGNNCC 5 cut(s) 238, 385, 433, 739, 840
PspPI GGNCC 5 cut(s) 397, 457, 941, 1152, 1204
PstI CTGCAG 1 cut(s) 190
PstNI CAGNNNCTG 1 cut(s) 944
PsuI RGATCY 1 cut(s) 383
PvuII CAGCTG 1 cut(s) 1097
RsaI GTAC 4 cut(s) 478, 536, 1003, 1177
RsaNI GTAC 4 cut(s) 477, 535, 1002, 1176
Sau3AI GATC 3 cut(s) 211, 383, 1225
Sau96I GGNCC 5 cut(s) 397, 457, 941, 1152, 1204
SchI GAGTC 1 cut(s) 917
ScrFI CCNGG 3 cut(s) 327, 347, 419
SduI GDGCHC 2 cut(s) 436, 841
SfaNI GCATC 9 cut(s) 158, 166, 262, 544, 690, 703, 740, 824, 1030
SfcI CTRYAG 2 cut(s) 186, 1083
SfoI GGCGCC 1 cut(s) 739
SinI GGWCC 1 cut(s) 1204
SmlI CTYRAG 3 cut(s) 104, 603, 742
SmoI CTYRAG 3 cut(s) 104, 603, 742
SsiI CCGC 6 cut(s) 9, 56, 111, 135, 151, 1150
SspDI GGCGCC 1 cut(s) 737
SspMI CTAG 2 cut(s) 131, 585
StyD4I CCNGG 3 cut(s) 325, 345, 417
StyI CCWWGG 3 cut(s) 366, 453, 1155
TaaI ACNGT 2 cut(s) 694, 800
TaiI ACGT 4 cut(s) 47, 66, 973, 1109
TatI WGTACW 3 cut(s) 534, 1001, 1175
TauI GCSGC 3 cut(s) 11, 137, 1153
TfiI GAWTC 2 cut(s) 581, 626
TscAI CASTG 1 cut(s) 765
TseFI GTSAC 1 cut(s) 502
TseI GCWGC 8 cut(s) 5, 188, 353, 470, 788, 899, 1094, 1097
Tsp45I GTSAC 1 cut(s) 502
TspDTI ATGAA 2 cut(s) 902, 974
TspGWI ACGGA 1 cut(s) 303
TspRI CASTG 1 cut(s) 765
VpaK11BI GGWCC 1 cut(s) 1204
XagI CCTNNNNNAGG 1 cut(s) 152
XapI RAATTY 3 cut(s) 292, 574, 1020
XbaI TCTAGA 1 cut(s) 584
XcmI CCANNNNNNNNNTGG 1 cut(s) 49
XspI CTAG 2 cut(s) 131, 585
Zsp2I ATGCAT 1 cut(s) 173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.