RLG00000028265

threonine aspartase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0004496
Physical Location & Seq
Reverse (-)
20684075 .. 20688332
4258 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028265

Sequence Viewer

Length: 951 bp
ATGGCTGCTGCCGCTAAACAAGTACAACAGCAACTACTAGAAGATGAAAACGACAGATTCTTCGTCGCCGTTCATGTCGGCGCTGGATACCACGCGCCTTCCAACGAGAAGGCCCTCAGGTCCGCCATGAAACGCGCTTGCCTCGCCGCCGCTTCAAATCTCCGCCAGGGCTCTGGTAGGTGCATAGATGCTGTCACTGCAGCTATTCAAGTGTTGGAGGATGATCCAATCACGAACGCGGGCCGGGGGTCTAATTTGACTGAAGATGGTCGTGTAGAATGTGATGCCAGTATTATGGATGGGAATTCTGGAGTATTTGGAGCTGTTGGAGCATTGTCAGGTGTCAAAAACCCCATCAAGGTTGCTGCTTTACTGGCGAAGGAGCAAATGATGGGATCCTCCCTGTTGGGTCGAATACCTCCCATCTTCCTCGTTGGTGAAGGTGCCCGCATATGGGCGAAGTCCAAGGGCATTGCTTTGTCGGCAAGCACATCAGCTGCTGATGAGTGGCTGGTGACCGAAAGGGCAAAAGCACATTGGAAAAAGTACAAAGCAATGGTAGATGATGCAAAAGCAAAGACAGAAACTTCTGCTGTTGGACATCCTTGTAGTCCTCAAGAGGCTGCTGGAATTCCAGAATTACAAGCTCAACCATGTGATTCTTTGAAGAAAAATGGGGCAGAGGAGGATTGGATCATGGACACTGTTGGAGTTATTTGTGTGGATGCTGATGGACAAATTGCATCTGGTGCTTCAAGTGGGGGCATTGCATTGAAGGTTGGTGGGCGGGTGGGGTTAGCAGCAATGCATGGTTCAGGCTGTTGGGCATCCTCCAAAGGTCTTTTTGGGGCTCCATGTATAGTTGGCTGTTGTGTAAGTGGTGCTGGAGAATATCTGATGAAAGGATTTGCAGCTCGAGAGTGCTGTGTCTCTTTATCACTGTATGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Asparaginase_2

Protein Analysis

317

Amino Acids

32.59

Weight (kDa)

6.23

Isoelectric Point (pI)

25.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asparaginase_2 PF01112 21 - 311 8.2e-47 Asparaginase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 443
AccII CGCG 3 cut(s) 95, 135, 239
AciI CCGC 8 cut(s) 12, 123, 147, 150, 163, 239, 448, 787
AclWI GGATC 4 cut(s) 218, 390, 403, 701
AcsI RAATTY 2 cut(s) 304, 630
AcuI CTGAAG 1 cut(s) 282
AfaI GTAC 2 cut(s) 24, 548
AfiI CCNNNNNNNGG 3 cut(s) 358, 453, 454
AgsI TTSAA 5 cut(s) 156, 209, 667, 756, 775
AjnI CCWGG 1 cut(s) 165
AluBI AGCT 5 cut(s) 203, 323, 497, 647, 914
AluI AGCT 5 cut(s) 203, 323, 497, 647, 914
Alw26I GTCTC 1 cut(s) 934
AlwI GGATC 4 cut(s) 218, 390, 403, 701
AlwNI CAGNNNCTG 1 cut(s) 500
Ama87I CYCGRG 1 cut(s) 915
AoxI GGCC 2 cut(s) 111, 241
ApeKI GCWGC 8 cut(s) 5, 8, 200, 365, 497, 623, 800, 911
ApoI RAATTY 2 cut(s) 304, 630
ArsI GACNNNNNNTTYG 2 cut(s) 44, 76
AspLEI GCGC 3 cut(s) 83, 97, 137
AspS9I GGNCC 3 cut(s) 112, 120, 241
AsuC2I CCSGG 1 cut(s) 245
AsuHPI GGTGA 2 cut(s) 449, 526
AvaI CYCGRG 1 cut(s) 915
AvaII GGWCC 1 cut(s) 120
AxyI CCTNAGG 1 cut(s) 116
BaeGI GKGCMC 1 cut(s) 448
BamHI GGATCC 1 cut(s) 395
BanI GGYRCC 1 cut(s) 443
BanII GRGCYC 2 cut(s) 173, 853
BbvI GCAGC 6 cut(s) 212, 352, 484, 610, 812, 923
BccI CCATC 6 cut(s) 260, 293, 362, 385, 431, 725
BceAI ACGGC 1 cut(s) 53
BciT130I CCWGG 1 cut(s) 167
BciVI GTATCC 1 cut(s) 80
BcnI CCSGG 1 cut(s) 245
BcoDI GTCTC 1 cut(s) 934
BfaI CTAG 1 cut(s) 38
BfmI CTRYAG 1 cut(s) 198
BfoI RGCGCY 1 cut(s) 84
BfuI GTATCC 1 cut(s) 80
Bme1390I CCNGG 2 cut(s) 167, 245
Bme18I GGWCC 1 cut(s) 120
BmeT110I CYCGRG 1 cut(s) 915
BmgT120I GGNCC 3 cut(s) 112, 120, 241
BmiI GGNNCC 3 cut(s) 397, 445, 852
BmrFI CCNGG 2 cut(s) 167, 245
BmsI GCATC 6 cut(s) 178, 274, 556, 715, 752, 836
BpmI CTGGAG 2 cut(s) 330, 906
BpuEI CTTGAG 1 cut(s) 600
BpuMI CCSGG 1 cut(s) 245
BsaJI CCNNGG 3 cut(s) 166, 244, 465
Bsc4I CCNNNNNNNGG 3 cut(s) 358, 453, 454
Bse1I ACTGG 2 cut(s) 288, 378
Bse21I CCTNAGG 1 cut(s) 116
Bse3DI GCAATG 4 cut(s) 471, 561, 765, 810
BseBI CCWGG 1 cut(s) 167
BseDI CCNNGG 3 cut(s) 166, 244, 465
BseGI GGATG 5 cut(s) 226, 304, 601, 730, 827
BseLI CCNNNNNNNGG 3 cut(s) 358, 453, 454
BseMI GCAATG 4 cut(s) 471, 561, 765, 810
BseMII CTCAG 1 cut(s) 130
BseNI ACTGG 2 cut(s) 288, 378
BseRI GAGGAG 1 cut(s) 698
BseSI GKGCMC 1 cut(s) 448
BseXI GCAGC 6 cut(s) 212, 352, 484, 610, 812, 923
Bsh1236I CGCG 3 cut(s) 95, 135, 239
BshFI GGCC 2 cut(s) 113, 243
BshNI GGYRCC 1 cut(s) 443
BsiHKCI CYCGRG 1 cut(s) 915
BsiSI CCGG 1 cut(s) 244
BslI CCNNNNNNNGG 3 cut(s) 358, 453, 454
BsmAI GTCTC 1 cut(s) 934
BsnI GGCC 2 cut(s) 113, 243
BsoBI CYCGRG 1 cut(s) 915
Bsp1286I GDGCHC 3 cut(s) 173, 448, 853
Bsp143I GATC 3 cut(s) 223, 395, 693
BspACI CCGC 8 cut(s) 12, 123, 147, 150, 163, 239, 448, 787
BspANI GGCC 2 cut(s) 113, 243
BspCNI CTCAG 1 cut(s) 129
BspFNI CGCG 3 cut(s) 95, 135, 239
BspLI GGNNCC 3 cut(s) 397, 445, 852
BspMAI CTGCAG 1 cut(s) 202
BspPI GGATC 4 cut(s) 218, 390, 403, 701
BspT107I GGYRCC 1 cut(s) 443
BsrDI GCAATG 4 cut(s) 471, 561, 765, 810
BsrI ACTGG 2 cut(s) 288, 378
BssECI CCNNGG 3 cut(s) 166, 244, 465
BssMI GATC 3 cut(s) 223, 395, 693
BssT1I CCWWGG 1 cut(s) 465
Bst2UI CCWGG 1 cut(s) 167
Bst4CI ACNGT 2 cut(s) 706, 942
BstAPI GCANNNNNTGC 1 cut(s) 749
BstC8I GCNNGC 4 cut(s) 139, 241, 448, 487
BstDEI CTNAG 1 cut(s) 116
BstEII GGTNACC 1 cut(s) 514
BstF5I GGATG 5 cut(s) 226, 304, 601, 730, 827
BstFNI CGCG 3 cut(s) 95, 135, 239
BstH2I RGCGCY 1 cut(s) 84
BstHHI GCGC 3 cut(s) 83, 97, 137
BstKTI GATC 3 cut(s) 226, 398, 696
BstMAI GTCTC 1 cut(s) 934
BstMBI GATC 3 cut(s) 223, 395, 693
BstMWI GCNNNNNNNGC 7 cut(s) 11, 143, 197, 329, 374, 482, 749
BstNI CCWGG 1 cut(s) 167
BstPI GGTNACC 1 cut(s) 514
BstSCI CCNGG 2 cut(s) 165, 243
BstSFI CTRYAG 1 cut(s) 198
BstSLI GKGCMC 1 cut(s) 448
BstUI CGCG 3 cut(s) 95, 135, 239
BstV1I GCAGC 6 cut(s) 212, 352, 484, 610, 812, 923
BstX2I RGATCY 1 cut(s) 395
BstXI CCANNNNNNTGG 2 cut(s) 173, 295
BstYI RGATCY 1 cut(s) 395
Bsu36I CCTNAGG 1 cut(s) 116
BsuI GTATCC 1 cut(s) 80
BsuRI GGCC 2 cut(s) 113, 243
BtsCI GGATG 5 cut(s) 226, 304, 601, 730, 827
BtsI GCAGTG 1 cut(s) 195
BtsIMutI CAGTG 3 cut(s) 195, 702, 938
Cac8I GCNNGC 4 cut(s) 139, 241, 448, 487
CaiI CAGNNNCTG 1 cut(s) 500
CfoI GCGC 3 cut(s) 83, 97, 137
Cfr13I GGNCC 3 cut(s) 112, 120, 241
Csp6I GTAC 2 cut(s) 23, 547
CviAII CATG 6 cut(s) 74, 127, 654, 697, 809, 855
CviQI GTAC 2 cut(s) 23, 547
DdeI CTNAG 1 cut(s) 116
DpnI GATC 3 cut(s) 225, 397, 695
DpnII GATC 3 cut(s) 223, 395, 693
EciI GGCGGA 2 cut(s) 112, 152
Eco130I CCWWGG 1 cut(s) 465
Eco24I GRGCYC 2 cut(s) 173, 853
Eco47I GGWCC 1 cut(s) 120
Eco57I CTGAAG 1 cut(s) 282
Eco81I CCTNAGG 1 cut(s) 116
Eco88I CYCGRG 1 cut(s) 915
Eco91I GGTNACC 1 cut(s) 514
EcoO109I RGGNCCY 1 cut(s) 112
EcoO65I GGTNACC 1 cut(s) 514
EcoRI GAATTC 2 cut(s) 304, 630
EcoRII CCWGG 1 cut(s) 165
EcoT14I CCWWGG 1 cut(s) 465
EcoT22I ATGCAT 1 cut(s) 810
EcoT38I GRGCYC 2 cut(s) 173, 853
ErhI CCWWGG 1 cut(s) 465
FaeI CATG 6 cut(s) 77, 130, 657, 700, 812, 858
FatI CATG 6 cut(s) 73, 126, 653, 696, 808, 854
FauI CCCGC 3 cut(s) 232, 455, 780
FauNDI CATATG 1 cut(s) 452
FokI GGATG 5 cut(s) 233, 311, 588, 737, 814
FriOI GRGCYC 2 cut(s) 173, 853
FspBI CTAG 1 cut(s) 38
GlaI GCGC 3 cut(s) 82, 96, 136
GsuI CTGGAG 2 cut(s) 330, 906
HaeII RGCGCY 1 cut(s) 84
HaeIII GGCC 2 cut(s) 113, 243
HapII CCGG 1 cut(s) 244
HhaI GCGC 3 cut(s) 83, 97, 137
Hin1II CATG 6 cut(s) 77, 130, 657, 700, 812, 858
Hin6I GCGC 3 cut(s) 81, 95, 135
HinP1I GCGC 3 cut(s) 81, 95, 135
HinfI GANTC 2 cut(s) 57, 659
HpaII CCGG 1 cut(s) 244
HphI GGTGA 2 cut(s) 449, 526
Hpy188I TCNGA 1 cut(s) 897
Hpy188III TCNNGA 5 cut(s) 232, 309, 617, 635, 917
Hpy99I CGWCG 1 cut(s) 68
HpyAV CCTTC 5 cut(s) 103, 108, 373, 434, 769
HpyCH4III ACNGT 2 cut(s) 706, 942
HpyCH4V TGCA 7 cut(s) 183, 200, 569, 743, 770, 808, 911
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 143, 197, 329, 374, 482, 749
HpyF3I CTNAG 1 cut(s) 116
Hsp92II CATG 6 cut(s) 77, 130, 657, 700, 812, 858
HspAI GCGC 3 cut(s) 81, 95, 135
Kzo9I GATC 3 cut(s) 223, 395, 693
LmnI GCTCC 4 cut(s) 320, 329, 382, 856
Lsp1109I GCAGC 6 cut(s) 212, 352, 484, 610, 812, 923
LweI GCATC 6 cut(s) 178, 274, 556, 715, 752, 836
MaeI CTAG 1 cut(s) 38
MaeIII GTNAC 2 cut(s) 193, 514
MalI GATC 3 cut(s) 225, 397, 695
MboI GATC 3 cut(s) 223, 395, 693
MboII GAAGA 5 cut(s) 52, 53, 275, 418, 679
MflI RGATCY 1 cut(s) 395
MhlI GDGCHC 3 cut(s) 173, 448, 853
MluCI AATT 5 cut(s) 253, 304, 630, 638, 738
MmeI TCCRAC 5 cut(s) 126, 195, 307, 577, 688
Mph1103I ATGCAT 1 cut(s) 810
MspA1I CMGCKG 1 cut(s) 497
MspI CCGG 1 cut(s) 244
MspR9I CCNGG 2 cut(s) 167, 245
MvaI CCWGG 1 cut(s) 167
MvnI CGCG 3 cut(s) 95, 135, 239
MwoI GCNNNNNNNGC 7 cut(s) 11, 143, 197, 329, 374, 482, 749
NciI CCSGG 1 cut(s) 245
NdeI CATATG 1 cut(s) 452
NdeII GATC 3 cut(s) 223, 395, 693
NlaIII CATG 6 cut(s) 77, 130, 657, 700, 812, 858
NlaIV GGNNCC 3 cut(s) 397, 445, 852
NmuCI GTSAC 2 cut(s) 193, 514
NsiI ATGCAT 1 cut(s) 810
PaeR7I CTCGAG 1 cut(s) 915
PfeI GAWTC 2 cut(s) 57, 659
Psp6I CCWGG 1 cut(s) 165
PspEI GGTNACC 1 cut(s) 514
PspGI CCWGG 1 cut(s) 165
PspN4I GGNNCC 3 cut(s) 397, 445, 852
PspPI GGNCC 3 cut(s) 112, 120, 241
PstI CTGCAG 1 cut(s) 202
PstNI CAGNNNCTG 1 cut(s) 500
PsuI RGATCY 1 cut(s) 395
PvuII CAGCTG 1 cut(s) 497
RsaI GTAC 2 cut(s) 24, 548
RsaNI GTAC 2 cut(s) 23, 547
Sau3AI GATC 3 cut(s) 223, 395, 693
Sau96I GGNCC 3 cut(s) 112, 120, 241
ScrFI CCNGG 2 cut(s) 167, 245
SduI GDGCHC 3 cut(s) 173, 448, 853
SfaNI GCATC 6 cut(s) 178, 274, 556, 715, 752, 836
SfcI CTRYAG 1 cut(s) 198
Sfr274I CTCGAG 1 cut(s) 915
SinI GGWCC 1 cut(s) 120
SlaI CTCGAG 1 cut(s) 915
SmlI CTYRAG 2 cut(s) 615, 915
SmoI CTYRAG 2 cut(s) 615, 915
Sse9I AATT 5 cut(s) 253, 304, 630, 638, 738
SsiI CCGC 8 cut(s) 12, 123, 147, 150, 163, 239, 448, 787
SspMI CTAG 1 cut(s) 38
StyD4I CCNGG 2 cut(s) 165, 243
StyI CCWWGG 1 cut(s) 465
TaaI ACNGT 2 cut(s) 706, 942
TaqI TCGA 2 cut(s) 412, 916
TaqII GACCGA 1 cut(s) 533
TasI AATT 5 cut(s) 253, 304, 630, 638, 738
TatI WGTACW 2 cut(s) 22, 546
TauI GCSGC 3 cut(s) 14, 149, 152
TfiI GAWTC 2 cut(s) 57, 659
TscAI CASTG 3 cut(s) 202, 709, 945
TseFI GTSAC 2 cut(s) 193, 514
TseI GCWGC 8 cut(s) 5, 8, 200, 365, 497, 623, 800, 911
Tsp45I GTSAC 2 cut(s) 193, 514
TspDTI ATGAA 4 cut(s) 60, 62, 143, 914
TspRI CASTG 3 cut(s) 202, 709, 945
VpaK11BI GGWCC 1 cut(s) 120
XapI RAATTY 2 cut(s) 304, 630
XhoI CTCGAG 1 cut(s) 915
XspI CTAG 1 cut(s) 38
Zsp2I ATGCAT 1 cut(s) 810
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.