RchiOBHm_Chr1g0353681

threonine aspartase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0004496
Physical Location & Seq
Forward (+)
46872411 .. 46872607
197 bp
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UTR
Exon/CDS
Intron
PRQ57930

Sequence Viewer

Length: 195 bp
ATGGCTGCTGCCGCTAAACAAGTACAACAGCAACTACTAGAAGATGAAAACGACAGATTCTTCGTCGCCGTTCATGTCGGCGCTGGATACCACGCGCCTTCCAACGAGAAGGCCCTCAGGTCCGCCATGAAACGCGCTTGCCTCGCCGCCGCTTCCATTCTCCGCCAGGTCTCTCTTGATCAATTCCCATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Asparaginase_2

Protein Analysis

64

Amino Acids

7.02

Weight (kDa)

7.96

Isoelectric Point (pI)

63.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 95, 135
AciI CCGC 5 cut(s) 12, 123, 147, 150, 163
AfaI GTAC 1 cut(s) 24
AjnI CCWGG 1 cut(s) 165
Alw26I GTCTC 1 cut(s) 175
AoxI GGCC 1 cut(s) 111
ApeKI GCWGC 2 cut(s) 5, 8
ArsI GACNNNNNNTTYG 2 cut(s) 44, 76
AspLEI GCGC 3 cut(s) 83, 97, 137
AspS9I GGNCC 2 cut(s) 112, 120
AvaII GGWCC 1 cut(s) 120
AxyI CCTNAGG 1 cut(s) 116
BceAI ACGGC 1 cut(s) 53
BciT130I CCWGG 1 cut(s) 167
BciVI GTATCC 1 cut(s) 80
BclI TGATCA 1 cut(s) 178
BcoDI GTCTC 1 cut(s) 175
BfaI CTAG 1 cut(s) 38
BfoI RGCGCY 1 cut(s) 84
BfuI GTATCC 1 cut(s) 80
BisI GCNGC 5 cut(s) 6, 9, 12, 147, 150
BlsI GCNGC 5 cut(s) 7, 10, 13, 148, 151
Bme1390I CCNGG 1 cut(s) 167
Bme18I GGWCC 1 cut(s) 120
BmgT120I GGNCC 2 cut(s) 112, 120
BmrFI CCNGG 1 cut(s) 167
BsaI GGTCTC 1 cut(s) 175
Bse21I CCTNAGG 1 cut(s) 116
BseBI CCWGG 1 cut(s) 167
BseMII CTCAG 1 cut(s) 130
Bsh1236I CGCG 2 cut(s) 95, 135
BshFI GGCC 1 cut(s) 113
BsmAI GTCTC 1 cut(s) 175
BsnI GGCC 1 cut(s) 113
Bso31I GGTCTC 1 cut(s) 175
Bsp143I GATC 1 cut(s) 178
BspACI CCGC 5 cut(s) 12, 123, 147, 150, 163
BspANI GGCC 1 cut(s) 113
BspCNI CTCAG 1 cut(s) 129
BspFNI CGCG 2 cut(s) 95, 135
BspTNI GGTCTC 1 cut(s) 175
BssMI GATC 1 cut(s) 178
Bst2UI CCWGG 1 cut(s) 167
BstC8I GCNNGC 1 cut(s) 139
BstDEI CTNAG 1 cut(s) 116
BstFNI CGCG 2 cut(s) 95, 135
BstH2I RGCGCY 1 cut(s) 84
BstHHI GCGC 3 cut(s) 83, 97, 137
BstKTI GATC 1 cut(s) 181
BstMAI GTCTC 1 cut(s) 175
BstMBI GATC 1 cut(s) 178
BstMWI GCNNNNNNNGC 2 cut(s) 11, 143
BstNI CCWGG 1 cut(s) 167
BstSCI CCNGG 1 cut(s) 165
BstUI CGCG 2 cut(s) 95, 135
Bsu36I CCTNAGG 1 cut(s) 116
BsuI GTATCC 1 cut(s) 80
BsuRI GGCC 1 cut(s) 113
Cac8I GCNNGC 1 cut(s) 139
CfoI GCGC 3 cut(s) 83, 97, 137
Cfr13I GGNCC 2 cut(s) 112, 120
Csp6I GTAC 1 cut(s) 23
CviAII CATG 2 cut(s) 74, 127
CviJI RGCY 2 cut(s) 5, 113
CviKI_1 RGCY 2 cut(s) 5, 113
CviQI GTAC 1 cut(s) 23
DdeI CTNAG 1 cut(s) 116
DpnI GATC 1 cut(s) 180
DpnII GATC 1 cut(s) 178
EciI GGCGGA 2 cut(s) 112, 152
Eco31I GGTCTC 1 cut(s) 175
Eco47I GGWCC 1 cut(s) 120
Eco81I CCTNAGG 1 cut(s) 116
EcoO109I RGGNCCY 1 cut(s) 112
EcoRII CCWGG 1 cut(s) 165
FaeI CATG 2 cut(s) 77, 130
FaiI YATR 2 cut(s) 75, 128
FatI CATG 2 cut(s) 73, 126
FbaI TGATCA 1 cut(s) 178
Fnu4HI GCNGC 5 cut(s) 6, 9, 12, 147, 150
Fsp4HI GCNGC 5 cut(s) 6, 9, 12, 147, 150
FspBI CTAG 1 cut(s) 38
GlaI GCGC 3 cut(s) 82, 96, 136
GluI GCNGC 5 cut(s) 6, 9, 12, 147, 150
HaeII RGCGCY 1 cut(s) 84
HaeIII GGCC 1 cut(s) 113
HhaI GCGC 3 cut(s) 83, 97, 137
Hin1II CATG 2 cut(s) 77, 130
Hin6I GCGC 3 cut(s) 81, 95, 135
HinP1I GCGC 3 cut(s) 81, 95, 135
HinfI GANTC 1 cut(s) 57
Hpy188III TCNNGA 1 cut(s) 176
Hpy99I CGWCG 1 cut(s) 68
HpyAV CCTTC 2 cut(s) 103, 108
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 143
HpyF3I CTNAG 1 cut(s) 116
Hsp92II CATG 2 cut(s) 77, 130
HspAI GCGC 3 cut(s) 81, 95, 135
Ksp22I TGATCA 1 cut(s) 178
Kzo9I GATC 1 cut(s) 178
LpnPI CCDG 4 cut(s) 69, 103, 152, 179
MaeI CTAG 1 cut(s) 38
MalI GATC 1 cut(s) 180
MboI GATC 1 cut(s) 178
MboII GAAGA 2 cut(s) 52, 53
MluCI AATT 1 cut(s) 182
MmeI TCCRAC 1 cut(s) 126
MnlI CCTC 2 cut(s) 125, 152
MspR9I CCNGG 1 cut(s) 167
MvaI CCWGG 1 cut(s) 167
MvnI CGCG 2 cut(s) 95, 135
MwoI GCNNNNNNNGC 2 cut(s) 11, 143
NdeII GATC 1 cut(s) 178
NlaIII CATG 2 cut(s) 77, 130
PfeI GAWTC 1 cut(s) 57
PkrI GCNGC 5 cut(s) 7, 10, 13, 148, 151
Psp6I CCWGG 1 cut(s) 165
PspGI CCWGG 1 cut(s) 165
PspPI GGNCC 2 cut(s) 112, 120
RsaI GTAC 1 cut(s) 24
RsaNI GTAC 1 cut(s) 23
SatI GCNGC 5 cut(s) 6, 9, 12, 147, 150
Sau3AI GATC 1 cut(s) 178
Sau96I GGNCC 2 cut(s) 112, 120
ScrFI CCNGG 1 cut(s) 167
SetI ASST 2 cut(s) 122, 171
SinI GGWCC 1 cut(s) 120
Sse9I AATT 1 cut(s) 182
SsiI CCGC 5 cut(s) 12, 123, 147, 150, 163
SspMI CTAG 1 cut(s) 38
StyD4I CCNGG 1 cut(s) 165
TasI AATT 1 cut(s) 182
TatI WGTACW 1 cut(s) 22
TauI GCSGC 3 cut(s) 14, 149, 152
TfiI GAWTC 1 cut(s) 57
TseI GCWGC 2 cut(s) 5, 8
TspDTI ATGAA 3 cut(s) 60, 62, 143
VpaK11BI GGWCC 1 cut(s) 120
XspI CTAG 1 cut(s) 38
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.