Rh1CG099800

threonine aspartase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0004496
Physical Location & Seq
Forward (+)
20985895 .. 20987635
1741 bp
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UTR
Exon/CDS
Intron
Rh1CG099800.1

Sequence Viewer

Length: 306 bp
ATGGTAGACGATGAAAAAGCAAAGATAGAAACTTATGTTGTTGGATTTCCTTATTGTCCTCAAGTGGCTGCTGGAATTTCAGAATTAGAAGCTCAACCATGTGATCCTTTGAAGAATAATGGGGCAGAGGAGGATTGCATCATGGACACTGTTGGAGTTATTTGTGTTGATGCTGACGAACAAATTGCATCTGGTGCCTCAAGTGGGGGCATTGCATTGAAGGTTGATGGGCGTGTAGGGTTAGCAGCAATGCATGGTTCAGGATGCCCAGCATCCTCCAAAGTCTTTTTGGGGCTCCATGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Asparaginase_2

Protein Analysis

101

Amino Acids

10.34

Weight (kDa)

4.31

Isoelectric Point (pI)

25.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asparaginase_2 PF01112 38 - 93 4.7e-12 Asparaginase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 194
AccI GTMKAC 1 cut(s) 6
AclWI GGATC 1 cut(s) 98
AcsI RAATTY 1 cut(s) 75
AfiI CCNNNNNNNGG 1 cut(s) 204
AgsI TTSAA 2 cut(s) 112, 220
AluBI AGCT 1 cut(s) 92
AluI AGCT 1 cut(s) 92
AlwI GGATC 1 cut(s) 98
ApeKI GCWGC 2 cut(s) 68, 245
ApoI RAATTY 1 cut(s) 75
BanI GGYRCC 1 cut(s) 194
BanII GRGCYC 1 cut(s) 297
BbvI GCAGC 2 cut(s) 55, 257
BccI CCATC 1 cut(s) 221
BcgI CGANNNNNNTGC 2 cut(s) 167, 201
BisI GCNGC 2 cut(s) 69, 246
BlsI GCNGC 2 cut(s) 70, 247
BmiI GGNNCC 2 cut(s) 196, 296
BmsI GCATC 5 cut(s) 147, 160, 197, 254, 281
BpuEI CTTGAG 2 cut(s) 45, 184
Bsc4I CCNNNNNNNGG 1 cut(s) 204
Bse3DI GCAATG 2 cut(s) 210, 255
BseGI GGATG 2 cut(s) 269, 272
BseLI CCNNNNNNNGG 1 cut(s) 204
BseMI GCAATG 2 cut(s) 210, 255
BseRI GAGGAG 1 cut(s) 143
BseXI GCAGC 2 cut(s) 55, 257
BseYI CCCAGC 1 cut(s) 268
BshNI GGYRCC 1 cut(s) 194
BslI CCNNNNNNNGG 1 cut(s) 204
Bsp1286I GDGCHC 1 cut(s) 297
Bsp143I GATC 1 cut(s) 103
BspLI GGNNCC 2 cut(s) 196, 296
BspPI GGATC 1 cut(s) 98
BspT107I GGYRCC 1 cut(s) 194
BsrDI GCAATG 2 cut(s) 210, 255
BssMI GATC 1 cut(s) 103
Bst4CI ACNGT 1 cut(s) 151
BstAPI GCANNNNNTGC 1 cut(s) 194
BstF5I GGATG 2 cut(s) 269, 272
BstKTI GATC 1 cut(s) 106
BstMBI GATC 1 cut(s) 103
BstMWI GCNNNNNNNGC 1 cut(s) 194
BstV1I GCAGC 2 cut(s) 55, 257
BtsCI GGATG 2 cut(s) 269, 272
BtsIMutI CAGTG 1 cut(s) 147
CviAII CATG 4 cut(s) 99, 142, 254, 299
CviJI RGCY 3 cut(s) 68, 92, 295
CviKI_1 RGCY 3 cut(s) 68, 92, 295
DpnI GATC 1 cut(s) 105
DpnII GATC 1 cut(s) 103
Eco24I GRGCYC 1 cut(s) 297
EcoT22I ATGCAT 1 cut(s) 255
EcoT38I GRGCYC 1 cut(s) 297
FaeI CATG 4 cut(s) 102, 145, 257, 302
FaiI YATR 6 cut(s) 36, 100, 143, 255, 300, 304
FatI CATG 4 cut(s) 98, 141, 253, 298
FblI GTMKAC 1 cut(s) 6
Fnu4HI GCNGC 2 cut(s) 69, 246
FokI GGATG 2 cut(s) 259, 276
FriOI GRGCYC 1 cut(s) 297
Fsp4HI GCNGC 2 cut(s) 69, 246
GluI GCNGC 2 cut(s) 69, 246
GsaI CCCAGC 1 cut(s) 272
Hin1II CATG 4 cut(s) 102, 145, 257, 302
Hpy166II GTNNAC 1 cut(s) 7
Hpy188I TCNGA 1 cut(s) 82
Hpy188III TCNNGA 1 cut(s) 261
Hpy8I GTNNAC 1 cut(s) 7
HpyAV CCTTC 1 cut(s) 214
HpyCH4III ACNGT 1 cut(s) 151
HpyCH4V TGCA 4 cut(s) 138, 188, 215, 253
HpyF10VI GCNNNNNNNGC 1 cut(s) 194
Hsp92II CATG 4 cut(s) 102, 145, 257, 302
Kzo9I GATC 1 cut(s) 103
LmnI GCTCC 1 cut(s) 300
LpnPI CCDG 4 cut(s) 57, 177, 246, 282
Lsp1109I GCAGC 2 cut(s) 55, 257
LweI GCATC 5 cut(s) 147, 160, 197, 254, 281
MalI GATC 1 cut(s) 105
MboI GATC 1 cut(s) 103
MboII GAAGA 1 cut(s) 124
MhlI GDGCHC 1 cut(s) 297
MluCI AATT 3 cut(s) 75, 83, 183
MmeI TCCRAC 2 cut(s) 22, 133
MnlI CCTC 5 cut(s) 69, 121, 124, 208, 286
Mph1103I ATGCAT 1 cut(s) 255
MwoI GCNNNNNNNGC 1 cut(s) 194
NdeII GATC 1 cut(s) 103
NlaIII CATG 4 cut(s) 102, 145, 257, 302
NlaIV GGNNCC 2 cut(s) 196, 296
NsiI ATGCAT 1 cut(s) 255
PkrI GCNGC 2 cut(s) 70, 247
PspFI CCCAGC 1 cut(s) 268
PspN4I GGNNCC 2 cut(s) 196, 296
SatI GCNGC 2 cut(s) 69, 246
Sau3AI GATC 1 cut(s) 103
SduI GDGCHC 1 cut(s) 297
SetI ASST 2 cut(s) 94, 225
SfaNI GCATC 5 cut(s) 147, 160, 197, 254, 281
SmlI CTYRAG 2 cut(s) 60, 199
SmoI CTYRAG 2 cut(s) 60, 199
Sse9I AATT 3 cut(s) 75, 83, 183
TaaI ACNGT 1 cut(s) 151
TasI AATT 3 cut(s) 75, 83, 183
TscAI CASTG 1 cut(s) 154
TseI GCWGC 2 cut(s) 68, 245
TspDTI ATGAA 1 cut(s) 27
TspRI CASTG 1 cut(s) 154
XapI RAATTY 1 cut(s) 75
XcmI CCANNNNNNNNNTGG 1 cut(s) 286
XmiI GTMKAC 1 cut(s) 6
Zsp2I ATGCAT 1 cut(s) 255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.