MD01G1053600.v1.1

threonine aspartase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0004496
Physical Location & Seq
Forward (+)
15834684 .. 15837623
2940 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1053600.v1.1.491

Sequence Viewer

Length: 1053 bp
ATGGGGGTAAGGCTAGCCGACATTCACCTCTCCCAGACCCTGCGTAAAGCGGGAGCCTTGTGCACTGGGTACGACATTTTTATTGTCTTAAGGGATGACCCAAGCAAAAATGCGGGCAGAGGCTCCAATTTGACAGAAGATGGTCGTGTAGAATGCGATGCCAGTATTATGGACGGAAATTCTGGAGTTTTTGGAGCTGTCGGAGCAATTCCAGGTGCCAGAAACCCCATCCAGGTCACTGCTTTATTGTCCAAGGAACAAATGATGGGATCCCCTCTCTTCCTGGTTGGTGAAGGTGCCCGTGCGTGGGCAAAGTCCAAGGGCCTTGCTTTGGCAGCAAGTACTACAGCTGATGATGAGTGGCTGGTGACAGAAAGATCGAAAGCACAATGGAGAAAGTACAAAGCAATGGTAGATGATGCAAAAGCAAATACAGGATTAGTAGCTCAACAATGTGATTCTTGGAAGCATAATGGAGCTGAAGAGGATTGCATCATGGATACTGTTGGAGTTATTTGTGTGGACACTGAAGGACAAATAGCATCTGGAGCCTCAAGTGGGGGAATTGCGCTTAAGGTGACAGATGCAATGCATGGTGCAGGGTGTTGGGGGTCCTCCAAGGGTCTTTTTGGTGCTCCGTGTATAGTTGGCTGTTGTGCAAGTGGTGCTGGAGAACATCTAATGAAAGGATTTGCAGCTCGCGAGCCCTGCGTCTCTTTGTCACTTTCACAGGAAGGTCCTGCCTCTGCTTGCACGAAAGTTTTATGCTCTGTTGTTCAAGAGAGTAATCACGATTGTACTGATAGAAGTGCCGGAATTCTACTTGTGCAAGCTGATGCTCCCATAATGGTTACAGGAAGGTCTCCAAAGTTGAAAGCTGTAGAGATTGGTGCTGCTTACACGTCCTTATCCTTTGGAATAGGATATTTTGGGAGCTCTATGGAGCGGCCCAAGGTTTCAATTCTTAGGAGTACAAAATTGCAAAGCAAAACCGGAGTGGATCATTTTGAGGCACGAATTGACATTTCTGGTGGAAAGCCGCCGTTACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Asparaginase_2

Protein Analysis

351

Amino Acids

36.45

Weight (kDa)

6.32

Isoelectric Point (pI)

29.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asparaginase_2 PF01112 29 - 263 3.5e-33 Asparaginase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 215, 296
AccBSI CCGCTC 1 cut(s) 946
AccII CGCG 1 cut(s) 702
AciI CCGC 4 cut(s) 50, 113, 946, 1040
AclWI GGATC 3 cut(s) 264, 277, 1008
AcsI RAATTY 2 cut(s) 178, 816
AcuI CTGAAG 2 cut(s) 501, 549
AfaI GTAC 5 cut(s) 71, 343, 401, 799, 973
AfiI CCNNNNNNNGG 5 cut(s) 232, 306, 307, 331, 558
AflII CTTAAG 2 cut(s) 88, 572
AflIII ACRYGT 1 cut(s) 900
AgsI TTSAA 3 cut(s) 779, 874, 960
AjiI CACGTC 1 cut(s) 903
AjnI CCWGG 3 cut(s) 211, 231, 282
AluBI AGCT 8 cut(s) 197, 350, 446, 479, 698, 833, 878, 936
AluI AGCT 8 cut(s) 197, 350, 446, 479, 698, 833, 878, 936
Alw21I GWGCWC 3 cut(s) 65, 637, 938
Alw26I GTCTC 2 cut(s) 718, 867
Alw44I GTGCAC 1 cut(s) 61
AlwI GGATC 3 cut(s) 264, 277, 1008
AlwNI CAGNNNCTG 1 cut(s) 40
AoxI GGCC 2 cut(s) 322, 947
ApaLI GTGCAC 1 cut(s) 61
ApeKI GCWGC 3 cut(s) 335, 695, 893
ApoI RAATTY 2 cut(s) 178, 816
AspLEI GCGC 1 cut(s) 571
AspS9I GGNCC 4 cut(s) 322, 612, 737, 948
AsuHPI GGTGA 4 cut(s) 17, 302, 379, 589
AsuNHI GCTAGC 1 cut(s) 13
AvaII GGWCC 2 cut(s) 612, 737
BaeGI GKGCMC 2 cut(s) 65, 301
BamHI GGATCC 1 cut(s) 269
BanI GGYRCC 2 cut(s) 215, 296
BanII GRGCYC 2 cut(s) 708, 938
Bbv12I GWGCWC 3 cut(s) 65, 637, 938
BbvI GCAGC 3 cut(s) 347, 707, 880
BccI CCATC 3 cut(s) 134, 236, 259
BceAI ACGGC 1 cut(s) 1027
BciT130I CCWGG 3 cut(s) 213, 233, 284
BciVI GTATCC 1 cut(s) 493
BcoDI GTCTC 2 cut(s) 718, 867
BfaI CTAG 1 cut(s) 14
BfmI CTRYAG 2 cut(s) 345, 879
BfrI CTTAAG 2 cut(s) 88, 572
BfuI GTATCC 1 cut(s) 493
BisI GCNGC 5 cut(s) 336, 696, 894, 947, 1040
BlsI GCNGC 5 cut(s) 337, 697, 895, 948, 1041
BmcAI AGTACT 1 cut(s) 343
Bme1390I CCNGG 3 cut(s) 213, 233, 284
Bme18I GGWCC 2 cut(s) 612, 737
BmgBI CACGTC 1 cut(s) 903
BmgT120I GGNCC 4 cut(s) 322, 612, 737, 948
BmiI GGNNCC 7 cut(s) 55, 124, 217, 271, 298, 550, 613
BmrFI CCNGG 3 cut(s) 213, 233, 284
BmrI ACTGGG 1 cut(s) 75
BmsI GCATC 6 cut(s) 148, 409, 501, 551, 574, 826
BmtI GCTAGC 1 cut(s) 17
BmuI ACTGGG 1 cut(s) 75
BpmI CTGGAG 3 cut(s) 204, 567, 690
BpuEI CTTGAG 1 cut(s) 538
BsaI GGTCTC 1 cut(s) 867
BsaJI CCNNGG 4 cut(s) 252, 318, 618, 951
BsaWI WCCGGW 1 cut(s) 992
Bsc4I CCNNNNNNNGG 5 cut(s) 232, 306, 307, 331, 558
Bse1I ACTGG 2 cut(s) 70, 162
Bse3DI GCAATG 2 cut(s) 414, 594
BseBI CCWGG 3 cut(s) 213, 233, 284
BseDI CCNNGG 4 cut(s) 252, 318, 618, 951
BseGI GGATG 2 cut(s) 100, 228
BseLI CCNNNNNNNGG 5 cut(s) 232, 306, 307, 331, 558
BseMI GCAATG 2 cut(s) 414, 594
BseNI ACTGG 2 cut(s) 70, 162
BseSI GKGCMC 2 cut(s) 65, 301
BseXI GCAGC 3 cut(s) 347, 707, 880
BsgI GTGCAG 1 cut(s) 618
Bsh1236I CGCG 1 cut(s) 702
BshFI GGCC 2 cut(s) 324, 949
BshNI GGYRCC 2 cut(s) 215, 296
BsiHKAI GWGCWC 3 cut(s) 65, 637, 938
BsiSI CCGG 2 cut(s) 813, 993
BslI CCNNNNNNNGG 5 cut(s) 232, 306, 307, 331, 558
BsmAI GTCTC 2 cut(s) 718, 867
BsmBI CGTCTC 1 cut(s) 718
BsmI GAATGC 1 cut(s) 158
BsnI GGCC 2 cut(s) 324, 949
Bso31I GGTCTC 1 cut(s) 867
Bsp1286I GDGCHC 5 cut(s) 65, 301, 637, 708, 938
Bsp143I GATC 3 cut(s) 269, 377, 1000
Bsp68I TCGCGA 1 cut(s) 702
BspACI CCGC 4 cut(s) 50, 113, 946, 1040
BspANI GGCC 2 cut(s) 324, 949
BspFNI CGCG 1 cut(s) 702
BspLI GGNNCC 7 cut(s) 55, 124, 217, 271, 298, 550, 613
BspOI GCTAGC 1 cut(s) 17
BspPI GGATC 3 cut(s) 264, 277, 1008
BspT107I GGYRCC 2 cut(s) 215, 296
BspTI CTTAAG 2 cut(s) 88, 572
BspTNI GGTCTC 1 cut(s) 867
BsrBI CCGCTC 1 cut(s) 946
BsrDI GCAATG 2 cut(s) 414, 594
BsrI ACTGG 2 cut(s) 70, 162
BssECI CCNNGG 4 cut(s) 252, 318, 618, 951
BssMI GATC 3 cut(s) 269, 377, 1000
BssT1I CCWWGG 4 cut(s) 252, 318, 618, 951
Bst2UI CCWGG 3 cut(s) 213, 233, 284
Bst4CI ACNGT 2 cut(s) 505, 1050
Bst6I CTCTTC 2 cut(s) 284, 477
BstAFI CTTAAG 2 cut(s) 88, 572
BstAPI GCANNNNNTGC 1 cut(s) 665
BstC8I GCNNGC 6 cut(s) 15, 115, 700, 704, 751, 831
BstDEI CTNAG 1 cut(s) 965
BstF5I GGATG 2 cut(s) 100, 228
BstFNI CGCG 1 cut(s) 702
BstHHI GCGC 1 cut(s) 571
BstKTI GATC 3 cut(s) 272, 380, 1003
BstMAI GTCTC 2 cut(s) 718, 867
BstMBI GATC 3 cut(s) 269, 377, 1000
BstMWI GCNNNNNNNGC 5 cut(s) 203, 335, 548, 665, 708
BstNI CCWGG 3 cut(s) 213, 233, 284
BstSCI CCNGG 3 cut(s) 211, 231, 282
BstSFI CTRYAG 2 cut(s) 345, 879
BstSLI GKGCMC 2 cut(s) 65, 301
BstUI CGCG 1 cut(s) 702
BstV1I GCAGC 3 cut(s) 347, 707, 880
BstX2I RGATCY 1 cut(s) 269
BstXI CCANNNNNNTGG 1 cut(s) 169
BstYI RGATCY 1 cut(s) 269
BsuI GTATCC 1 cut(s) 493
BsuRI GGCC 2 cut(s) 324, 949
BtgZI GCGATG 1 cut(s) 171
BtrI CACGTC 1 cut(s) 903
BtsCI GGATG 2 cut(s) 100, 228
BtsI GCAGTG 1 cut(s) 237
BtsIMutI CAGTG 3 cut(s) 63, 237, 525
BtuMI TCGCGA 1 cut(s) 702
Cac8I GCNNGC 6 cut(s) 15, 115, 700, 704, 751, 831
CaiI CAGNNNCTG 1 cut(s) 40
CfoI GCGC 1 cut(s) 571
Cfr13I GGNCC 4 cut(s) 322, 612, 737, 948
CseI GACGC 1 cut(s) 700
Csp6I GTAC 5 cut(s) 70, 342, 400, 798, 972
CviAII CATG 2 cut(s) 496, 593
CviQI GTAC 5 cut(s) 70, 342, 400, 798, 972
DdeI CTNAG 1 cut(s) 965
DpnI GATC 3 cut(s) 271, 379, 1002
DpnII GATC 3 cut(s) 269, 377, 1000
Eam1104I CTCTTC 2 cut(s) 284, 477
EarI CTCTTC 2 cut(s) 284, 477
Ecl136II GAGCTC 1 cut(s) 936
Eco130I CCWWGG 4 cut(s) 252, 318, 618, 951
Eco24I GRGCYC 2 cut(s) 708, 938
Eco31I GGTCTC 1 cut(s) 867
Eco47I GGWCC 2 cut(s) 612, 737
Eco53kI GAGCTC 1 cut(s) 936
Eco57I CTGAAG 2 cut(s) 501, 549
EcoICRI GAGCTC 1 cut(s) 936
EcoO109I RGGNCCY 3 cut(s) 322, 612, 737
EcoRI GAATTC 1 cut(s) 816
EcoRII CCWGG 3 cut(s) 211, 231, 282
EcoT14I CCWWGG 4 cut(s) 252, 318, 618, 951
EcoT22I ATGCAT 1 cut(s) 594
EcoT38I GRGCYC 2 cut(s) 708, 938
ErhI CCWWGG 4 cut(s) 252, 318, 618, 951
Esp3I CGTCTC 1 cut(s) 718
FaeI CATG 2 cut(s) 499, 596
FaiI YATR 8 cut(s) 170, 471, 497, 594, 644, 766, 845, 941
FatI CATG 2 cut(s) 495, 592
FauI CCCGC 2 cut(s) 43, 106
Fnu4HI GCNGC 5 cut(s) 336, 696, 894, 947, 1040
FokI GGATG 2 cut(s) 107, 215
FriOI GRGCYC 2 cut(s) 708, 938
Fsp4HI GCNGC 5 cut(s) 336, 696, 894, 947, 1040
FspBI CTAG 1 cut(s) 14
GlaI GCGC 1 cut(s) 570
GluI GCNGC 5 cut(s) 336, 696, 894, 947, 1040
GsuI CTGGAG 3 cut(s) 204, 567, 690
HaeIII GGCC 2 cut(s) 324, 949
HapII CCGG 2 cut(s) 813, 993
HgaI GACGC 1 cut(s) 700
HhaI GCGC 1 cut(s) 571
Hin1II CATG 2 cut(s) 499, 596
Hin6I GCGC 1 cut(s) 569
HinP1I GCGC 1 cut(s) 569
HinfI GANTC 1 cut(s) 458
HpaII CCGG 2 cut(s) 813, 993
HphI GGTGA 4 cut(s) 17, 302, 379, 589
Hpy166II GTNNAC 2 cut(s) 63, 523
Hpy188I TCNGA 1 cut(s) 203
Hpy188III TCNNGA 5 cut(s) 183, 546, 701, 779, 791
Hpy8I GTNNAC 2 cut(s) 63, 523
HpyAV CCTTC 4 cut(s) 287, 524, 728, 852
HpyCH4III ACNGT 2 cut(s) 505, 1050
HpyCH4IV ACGT 1 cut(s) 902
HpyF10VI GCNNNNNNNGC 5 cut(s) 203, 335, 548, 665, 708
HpyF3I CTNAG 1 cut(s) 965
HpySE526I ACGT 1 cut(s) 902
Hsp92II CATG 2 cut(s) 499, 596
HspAI GCGC 1 cut(s) 569
Kzo9I GATC 3 cut(s) 269, 377, 1000
Lsp1109I GCAGC 3 cut(s) 347, 707, 880
LweI GCATC 6 cut(s) 148, 409, 501, 551, 574, 826
MaeI CTAG 1 cut(s) 14
MaeII ACGT 1 cut(s) 902
MaeIII GTNAC 6 cut(s) 235, 367, 577, 720, 850, 1044
MalI GATC 3 cut(s) 271, 379, 1002
MbiI CCGCTC 1 cut(s) 946
MboI GATC 3 cut(s) 269, 377, 1000
MboII GAAGA 3 cut(s) 149, 271, 494
MflI RGATCY 1 cut(s) 269
MhlI GDGCHC 5 cut(s) 65, 301, 637, 708, 938
MluCI AATT 8 cut(s) 127, 178, 207, 564, 816, 960, 977, 1017
MmeI TCCRAC 2 cut(s) 181, 487
MnlI CCTC 8 cut(s) 38, 113, 285, 478, 562, 625, 754, 1003
Mph1103I ATGCAT 1 cut(s) 594
MseI TTAA 2 cut(s) 89, 573
MspA1I CMGCKG 1 cut(s) 350
MspCI CTTAAG 2 cut(s) 88, 572
MspI CCGG 2 cut(s) 813, 993
MspR9I CCNGG 3 cut(s) 213, 233, 284
Mva1269I GAATGC 1 cut(s) 158
MvaI CCWGG 3 cut(s) 213, 233, 284
MvnI CGCG 1 cut(s) 702
MwoI GCNNNNNNNGC 5 cut(s) 203, 335, 548, 665, 708
NdeII GATC 3 cut(s) 269, 377, 1000
NheI GCTAGC 1 cut(s) 13
NlaIII CATG 2 cut(s) 499, 596
NlaIV GGNNCC 7 cut(s) 55, 124, 217, 271, 298, 550, 613
NmuCI GTSAC 4 cut(s) 235, 367, 577, 720
NruI TCGCGA 1 cut(s) 702
NsiI ATGCAT 1 cut(s) 594
PctI GAATGC 1 cut(s) 158
PfeI GAWTC 1 cut(s) 458
PkrI GCNGC 5 cut(s) 337, 697, 895, 948, 1041
PpuMI RGGWCCY 2 cut(s) 612, 737
Psp124BI GAGCTC 1 cut(s) 938
Psp5II RGGWCCY 2 cut(s) 612, 737
Psp6I CCWGG 3 cut(s) 211, 231, 282
PspGI CCWGG 3 cut(s) 211, 231, 282
PspN4I GGNNCC 7 cut(s) 55, 124, 217, 271, 298, 550, 613
PspPI GGNCC 4 cut(s) 322, 612, 737, 948
PspPPI RGGWCCY 2 cut(s) 612, 737
PstNI CAGNNNCTG 1 cut(s) 40
PsuI RGATCY 1 cut(s) 269
PvuII CAGCTG 1 cut(s) 350
RruI TCGCGA 1 cut(s) 702
RsaI GTAC 5 cut(s) 71, 343, 401, 799, 973
RsaNI GTAC 5 cut(s) 70, 342, 400, 798, 972
SacI GAGCTC 1 cut(s) 938
SaqAI TTAA 2 cut(s) 89, 573
SatI GCNGC 5 cut(s) 336, 696, 894, 947, 1040
Sau3AI GATC 3 cut(s) 269, 377, 1000
Sau96I GGNCC 4 cut(s) 322, 612, 737, 948
ScaI AGTACT 1 cut(s) 343
ScrFI CCNGG 3 cut(s) 213, 233, 284
SduI GDGCHC 5 cut(s) 65, 301, 637, 708, 938
SfaNI GCATC 6 cut(s) 148, 409, 501, 551, 574, 826
SfcI CTRYAG 2 cut(s) 345, 879
SinI GGWCC 2 cut(s) 612, 737
SmlI CTYRAG 3 cut(s) 88, 553, 572
SmoI CTYRAG 3 cut(s) 88, 553, 572
Sse9I AATT 8 cut(s) 127, 178, 207, 564, 816, 960, 977, 1017
SsiI CCGC 4 cut(s) 50, 113, 946, 1040
SspMI CTAG 1 cut(s) 14
SstI GAGCTC 1 cut(s) 938
StyD4I CCNGG 3 cut(s) 211, 231, 282
StyI CCWWGG 4 cut(s) 252, 318, 618, 951
TaaI ACNGT 2 cut(s) 505, 1050
TaiI ACGT 1 cut(s) 905
TaqI TCGA 1 cut(s) 380
TasI AATT 8 cut(s) 127, 178, 207, 564, 816, 960, 977, 1017
TatI WGTACW 4 cut(s) 341, 399, 797, 971
TauI GCSGC 2 cut(s) 949, 1042
TfiI GAWTC 1 cut(s) 458
Tru1I TTAA 2 cut(s) 89, 573
Tru9I TTAA 2 cut(s) 89, 573
TscAI CASTG 3 cut(s) 70, 244, 532
TseFI GTSAC 4 cut(s) 235, 367, 577, 720
TseI GCWGC 3 cut(s) 335, 695, 893
Tsp45I GTSAC 4 cut(s) 235, 367, 577, 720
TspDTI ATGAA 1 cut(s) 698
TspGWI ACGGA 2 cut(s) 189, 627
TspRI CASTG 3 cut(s) 70, 244, 532
Vha464I CTTAAG 2 cut(s) 88, 572
VneI GTGCAC 1 cut(s) 61
VpaK11BI GGWCC 2 cut(s) 612, 737
XapI RAATTY 2 cut(s) 178, 816
XspI CTAG 1 cut(s) 14
ZrmI AGTACT 1 cut(s) 343
Zsp2I ATGCAT 1 cut(s) 594
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.