Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr6

Orthogroup ID

OG0004496

Physical Location & Seq

Genomic Coordinates Reverse (-)

39460647 .. 39466544

Gene Structure 5898 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000029492

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1539 bp

ATGGTAGACGATGAAAAAGCAAAGACAGAAACTTCTGTTGTTGGATTTCCTTGTAGTCCTCAAGAGGCTGCTGGAATTTCAAAATTAGAAGCTCAACCATGTGATCCTTTGAAGAATAATGGGGCAGAGGAGGATTGCATCATGGACACTGTTGGAGTTATTTGTGTCGATGCTGACGGACAAATTGCATCTGGTGCCTCAAGTGGGGGCATTGCATTAAAGGTTGGTGGGCGTGTAGGGTTAGCAGCAATGCATGGTTCAAGATGCTGGGCATCCTCCAAAGTCTTTTTGGGGCTCCATGCAGGTCCTGCCTGTGCTTGTATGAAAGTTTTACGCTCCTTTGTTCAAGAGAGTAATCAAAATTGTACTGATAAAAGTGCCGGATTTCTAGATGTGCAAGCTGATGCTCTTGTGATGGTTTCAGGAAGTTCTCCAAAGTTAAAAGCTATTGAGATTGAAGCTGCTTACTCTTCCTTATCCCTTGGGATAGGATTTTTCGGAAGCTCTATGGAGCGAACCAAGTTTTTGGCCTTCAGAAAATGGTTCTTTCGCTACTTGAGGTTTGAAAGTAATGCTCTTTTTGTAGTGGTTCTGGTTGACTCAACTTGTTTGTGCATCAAATTTGCTTCAAAGGTTGCACCAACCGAGATCGAGTTTTCGGATGTGTATGCTGTTGAGCTGATCAACTATGGTGTGATTCATGGACCAAATGTCTCAAATGCTCGAAGATGCCTTAAGGCCTTAAGGATTTACCGCTTTACGGTGCATAGTTTCCAGAGCTCAAGGACTTTGCCGTCTGTTCGGGTCCTTGCTACATATACCTTTGGTCATAAGGATCTCCACACATGTCAGATATGGGTCAATCAAATCAATGCTTCTCTGGACTTAGAGCAGGGGACGCCAAAGAATCTTTTGGTTTTTGTTCATCCAAGGAGTGGGAAGGGAAATGCCTGCAAAACTTGGGACAGTGTGGCTCGTATTTTCTCCCGTGCTAAAGTGAAAACAAAGGTGATTGTGATCGAAAGGGGAGGGCATGCATATGATGCGATGGCAACTATAGGAAACAAGTATAAAGCTCCATATCCACCAACTCCTTCAGATTTCCTTGATTCTGCTTGTTGTAATGAGAATCTCATTGTAAATGATCCAAGTGAAACCGATATTGAAGCTTCTTCTCGGAATAATGAGCAGTCTCCCCTCCTCTCAAGTTCAATGGATCAAGAGCTTGAGATTTCCCTCCCCAATGAACATTCAAGATTTGGACTCATTCCTGCTGGCTCAACTGATGCCATTGTGATGTGTTCAACTGGGGCTCGAGATCCTATAACATCTGCATTTCACATTGTCCTTGGTAAAAGAGTGTGGCTTGATGTAGCCCAAGTTGTGAGGTGGAAAACGGAATCTACATCGGAGGTTGACCCTTACGTGCACTCTCTACAAGTGGCATTTGGTCCTGAAGCTCCTTCCTCGGGCTCCATGAGGACATATGGGGTACTGCGTACTGAAGGTAGAAAAATCTTGATCAAGGGAGCAGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001727 GO:0001729 GO:0003674 GO:0003824 GO:0005488 GO:0005509 GO:0006629 GO:0006643 GO:0006665 GO:0006672 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008219 GO:0009987 GO:0010941 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0030258 GO:0034641 GO:0043067 GO:0043069 GO:0043167 GO:0043169 GO:0043603 GO:0044237 GO:0044238 GO:0044255 GO:0046834 GO:0046872 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0060548 GO:0065007 GO:0071704 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00600 map00600

Pfam Domains

Protein Families

DAGK_cat

Protein Analysis

513

Amino Acids

55.45

Weight (kDa)

7.49

Isoelectric Point (pI)

44.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Asparaginase_2	PF01112	38 - 94	3.9e-11	Asparaginase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004496)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G00590 AT4G00590
fragaria_vesca	FvH4_7g13631 FvH4_7g13631
malus_domestica	MD01G1053500.v1.1 MD01G1053600.v1.1 MD07G1141600.v1.1
prunus_persica	Prupe.2G162500_v2.0.a1 Prupe.2G162500_v2.0.a1 Prupe.2G162500_v2.0.a1 Prupe.2G162500_v2.0.a1 Prupe.2G162500_v2.0.a1 Prupe.2G162500_v2.0.a1 Prupe.8G034000_v2.0.a1
pyrus_communis	pycom01g07920 pycom07g14050
rosa_chinensis	RchiOBHm_Chr1g0353681 RchiOBHm_Chr1g0353691
rosa_laevigata	RLG00000028242 RLG00000028265 RLG00000029492 RLG00000029775
rosa_multiflora	Rmu_sc0001849.1_g000001
rosa_roxburghii	Rroxscaffold_4G00301740
rosa_rugosa	Rorug01G0237100
rosa_samantha	Rh1AG249300 Rh1BG219400 Rh1CG099800 Rh1CG232000 Rh1DG107100 Rh1DG246400
rosa_wichuraiana	Rw1G021730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	793
Acc36I	ACCTGC	1 cut(s)	293
AccB1I	GGYRCC	1 cut(s)	194
AccB7I	CCANNNNNTGG	1 cut(s)	935
AccI	GTMKAC	1 cut(s)	6
AciI	CCGC	1 cut(s)	754
AclWI	GGATC	5 cut(s)	98, 843, 1139, 1224, 1313
AcsI	RAATTY	2 cut(s)	75, 620
AcuI	CTGAAG	4 cut(s)	517, 1080, 1476, 1524
AcyI	GRCGYC	1 cut(s)	899
AfaI	GTAC	3 cut(s)	367, 1494, 1501
AfiI	CCNNNNNNNGG	4 cut(s)	204, 760, 935, 1469
AflII	CTTAAG	2 cut(s)	734, 742
AflIII	ACRYGT	1 cut(s)	845
AhdI	GACNNNNNGTC	1 cut(s)	710
Alw21I	GWGCWC	2 cut(s)	782, 1431
Alw26I	GTCTC	2 cut(s)	718, 1197
Alw44I	GTGCAC	1 cut(s)	1427
AlwI	GGATC	5 cut(s)	98, 843, 1139, 1224, 1313
AlwNI	CAGNNNCTG	1 cut(s)	308
Ama87I	CYCGRG	2 cut(s)	1314, 1468
AoxI	GGCC	2 cut(s)	528, 738
ApaLI	GTGCAC	1 cut(s)	1427
ApeKI	GCWGC	4 cut(s)	68, 245, 461, 1532
ApoI	RAATTY	2 cut(s)	75, 620
AspS9I	GGNCC	4 cut(s)	305, 704, 805, 1451
AsuHPI	GGTGA	1 cut(s)	1021
AvaI	CYCGRG	2 cut(s)	1314, 1468
AvaII	GGWCC	4 cut(s)	305, 704, 805, 1451
BaeGI	GKGCMC	1 cut(s)	1431
BanI	GGYRCC	1 cut(s)	194
BanII	GRGCYC	4 cut(s)	297, 782, 1315, 1475
Bbv12I	GWGCWC	2 cut(s)	782, 1431
BbvI	GCAGC	3 cut(s)	55, 257, 448
BccI	CCATC	2 cut(s)	409, 1042
BceAI	ACGGC	1 cut(s)	778
BclI	TGATCA	2 cut(s)	681, 1521
BcoDI	GTCTC	2 cut(s)	718, 1197
BfaI	CTAG	1 cut(s)	389
BfmI	CTRYAG	1 cut(s)	1056
BfrI	CTTAAG	2 cut(s)	734, 742
BfuAI	ACCTGC	1 cut(s)	293
BisI	GCNGC	4 cut(s)	69, 246, 462, 1533
BlsI	GCNGC	4 cut(s)	70, 247, 463, 1534
Bme18I	GGWCC	4 cut(s)	305, 704, 805, 1451
BmeRI	GACNNNNNGTC	1 cut(s)	710
BmeT110I	CYCGRG	2 cut(s)	1314, 1468
BmgT120I	GGNCC	4 cut(s)	305, 704, 805, 1451
BmiI	GGNNCC	4 cut(s)	196, 296, 806, 1474
BmrI	ACTGGG	1 cut(s)	1317
BmuI	ACTGGG	1 cut(s)	1317
BpuEI	CTTGAG	6 cut(s)	45, 184, 577, 766, 1189, 1247
BsaAI	YACGTR	1 cut(s)	1426
BsaBI	GATNNNNATC	1 cut(s)	1016
BsaHI	GRCGYC	1 cut(s)	899
BsaJI	CCNNGG	4 cut(s)	481, 929, 1348, 1467
Bsc4I	CCNNNNNNNGG	4 cut(s)	204, 760, 935, 1469
Bse1I	ACTGG	1 cut(s)	1312
Bse3DI	GCAATG	2 cut(s)	210, 255
Bse8I	GATNNNNATC	1 cut(s)	1016
BseDI	CCNNGG	4 cut(s)	481, 929, 1348, 1467
BseGI	GGATG	3 cut(s)	272, 667, 925
BseJI	GATNNNNATC	1 cut(s)	1016
BseLI	CCNNNNNNNGG	4 cut(s)	204, 760, 935, 1469
BseMI	GCAATG	2 cut(s)	210, 255
BseNI	ACTGG	1 cut(s)	1312
BseRI	GAGGAG	2 cut(s)	143, 1190
BseSI	GKGCMC	1 cut(s)	1431
BseXI	GCAGC	3 cut(s)	55, 257, 448
BseYI	CCCAGC	1 cut(s)	267
BshFI	GGCC	2 cut(s)	530, 740
BshNI	GGYRCC	1 cut(s)	194
BsiHKAI	GWGCWC	2 cut(s)	782, 1431
BsiHKCI	CYCGRG	2 cut(s)	1314, 1468
BsiSI	CCGG	1 cut(s)	381
BslFI	GGGAC	2 cut(s)	910, 977
BslI	CCNNNNNNNGG	4 cut(s)	204, 760, 935, 1469
BsmAI	GTCTC	2 cut(s)	718, 1197
BsmFI	GGGAC	2 cut(s)	910, 977
BsnI	GGCC	2 cut(s)	530, 740
BsoBI	CYCGRG	2 cut(s)	1314, 1468
Bsp1286I	GDGCHC	5 cut(s)	297, 782, 1315, 1431, 1475
Bsp143I	GATC	9 cut(s)	103, 648, 681, 835, 1017, 1144, 1216, 1318, 1521
BspACI	CCGC	1 cut(s)	754
BspANI	GGCC	2 cut(s)	530, 740
BspLI	GGNNCC	4 cut(s)	196, 296, 806, 1474
BspMI	ACCTGC	1 cut(s)	293
BspPI	GGATC	5 cut(s)	98, 843, 1139, 1224, 1313
BspT107I	GGYRCC	1 cut(s)	194
BspTI	CTTAAG	2 cut(s)	734, 742
BsrDI	GCAATG	2 cut(s)	210, 255
BsrI	ACTGG	1 cut(s)	1312
BssECI	CCNNGG	4 cut(s)	481, 929, 1348, 1467
BssMI	GATC	9 cut(s)	103, 648, 681, 835, 1017, 1144, 1216, 1318, 1521
BssNI	GRCGYC	1 cut(s)	899
BssT1I	CCWWGG	3 cut(s)	481, 929, 1348
Bst4CI	ACNGT	3 cut(s)	151, 763, 968
Bst6I	CTCTTC	1 cut(s)	475
BstACI	GRCGYC	1 cut(s)	899
BstAFI	CTTAAG	2 cut(s)	734, 742
BstAPI	GCANNNNNTGC	3 cut(s)	194, 308, 1043
BstBAI	YACGTR	1 cut(s)	1426
BstC8I	GCNNGC	4 cut(s)	399, 952, 1035, 1276
BstDEI	CTNAG	2 cut(s)	886, 1536
BstF5I	GGATG	3 cut(s)	272, 667, 925
BstKTI	GATC	9 cut(s)	106, 651, 684, 838, 1020, 1147, 1219, 1321, 1524
BstMAI	GTCTC	2 cut(s)	718, 1197
BstMBI	GATC	9 cut(s)	103, 648, 681, 835, 1017, 1144, 1216, 1318, 1521
BstMWI	GCNNNNNNNGC	4 cut(s)	194, 308, 898, 1043
BstNSI	RCATGY	2 cut(s)	849, 1037
BstSFI	CTRYAG	1 cut(s)	1056
BstSLI	GKGCMC	1 cut(s)	1431
BstV1I	GCAGC	3 cut(s)	55, 257, 448
BstX2I	RGATCY	2 cut(s)	835, 1318
BstXI	CCANNNNNNTGG	1 cut(s)	526
BstYI	RGATCY	2 cut(s)	835, 1318
BsuRI	GGCC	2 cut(s)	530, 740
BtgZI	GCGATG	1 cut(s)	1061
BtsCI	GGATG	3 cut(s)	272, 667, 925
BtsIMutI	CAGTG	2 cut(s)	147, 973
BveI	ACCTGC	1 cut(s)	293
Cac8I	GCNNGC	4 cut(s)	399, 952, 1035, 1276
CaiI	CAGNNNCTG	1 cut(s)	308
Cfr13I	GGNCC	4 cut(s)	305, 704, 805, 1451
CseI	GACGC	1 cut(s)	907
Csp6I	GTAC	3 cut(s)	366, 1493, 1500
CviAII	CATG	8 cut(s)	99, 142, 254, 299, 701, 846, 1034, 1477
CviQI	GTAC	3 cut(s)	366, 1493, 1500
DdeI	CTNAG	2 cut(s)	886, 1536
DpnI	GATC	9 cut(s)	105, 650, 683, 837, 1019, 1146, 1218, 1320, 1523
DpnII	GATC	9 cut(s)	103, 648, 681, 835, 1017, 1144, 1216, 1318, 1521
DrdI	GACNNNNNNGTC	1 cut(s)	793
DriI	GACNNNNNGTC	1 cut(s)	710
DseDI	GACNNNNNNGTC	1 cut(s)	793
Eam1104I	CTCTTC	1 cut(s)	475
Eam1105I	GACNNNNNGTC	1 cut(s)	710
EarI	CTCTTC	1 cut(s)	475
Ecl136II	GAGCTC	1 cut(s)	780
Eco130I	CCWWGG	3 cut(s)	481, 929, 1348
Eco147I	AGGCCT	1 cut(s)	740
Eco24I	GRGCYC	4 cut(s)	297, 782, 1315, 1475
Eco47I	GGWCC	4 cut(s)	305, 704, 805, 1451
Eco53kI	GAGCTC	1 cut(s)	780
Eco57I	CTGAAG	4 cut(s)	517, 1080, 1476, 1524
Eco88I	CYCGRG	2 cut(s)	1314, 1468
EcoICRI	GAGCTC	1 cut(s)	780
EcoO109I	RGGNCCY	2 cut(s)	305, 805
EcoT14I	CCWWGG	3 cut(s)	481, 929, 1348
EcoT22I	ATGCAT	2 cut(s)	255, 1039
EcoT38I	GRGCYC	4 cut(s)	297, 782, 1315, 1475
ErhI	CCWWGG	3 cut(s)	481, 929, 1348
FaeI	CATG	8 cut(s)	102, 145, 257, 302, 704, 849, 1037, 1480
FaqI	GGGAC	2 cut(s)	910, 977
FatI	CATG	8 cut(s)	98, 141, 253, 298, 700, 845, 1033, 1476
FauNDI	CATATG	2 cut(s)	1039, 1486
FbaI	TGATCA	2 cut(s)	681, 1521
FblI	GTMKAC	1 cut(s)	6
Fnu4HI	GCNGC	4 cut(s)	69, 246, 462, 1533
FokI	GGATG	3 cut(s)	259, 674, 912
FriOI	GRGCYC	4 cut(s)	297, 782, 1315, 1475
Fsp4HI	GCNGC	4 cut(s)	69, 246, 462, 1533
FspBI	CTAG	1 cut(s)	389
GluI	GCNGC	4 cut(s)	69, 246, 462, 1533
GsaI	CCCAGC	1 cut(s)	271
HaeIII	GGCC	2 cut(s)	530, 740
HapII	CCGG	1 cut(s)	381
HgaI	GACGC	1 cut(s)	907
Hin1I	GRCGYC	1 cut(s)	899
Hin1II	CATG	8 cut(s)	102, 145, 257, 302, 704, 849, 1037, 1480
HincII	GTYRAC	2 cut(s)	598, 1417
HindII	GTYRAC	2 cut(s)	598, 1417
HindIII	AAGCTT	1 cut(s)	1167
HinfI	GANTC	7 cut(s)	599, 697, 907, 1109, 1129, 1263, 1400
HpaII	CCGG	1 cut(s)	381
HphI	GGTGA	1 cut(s)	1021
Hpy166II	GTNNAC	4 cut(s)	7, 598, 1417, 1429
Hpy188I	TCNGA	7 cut(s)	500, 536, 661, 852, 1099, 1179, 1411
Hpy8I	GTNNAC	4 cut(s)	7, 598, 1417, 1429
HpyAV	CCTTC	5 cut(s)	541, 934, 1104, 1473, 1499
HpyCH4III	ACNGT	3 cut(s)	151, 763, 968
HpyCH4IV	ACGT	1 cut(s)	1425
HpyF10VI	GCNNNNNNNGC	4 cut(s)	194, 308, 898, 1043
HpyF3I	CTNAG	2 cut(s)	886, 1536
HpySE526I	ACGT	1 cut(s)	1425
Hsp92I	GRCGYC	1 cut(s)	899
Hsp92II	CATG	8 cut(s)	102, 145, 257, 302, 704, 849, 1037, 1480
Ksp22I	TGATCA	2 cut(s)	681, 1521
Kzo9I	GATC	9 cut(s)	103, 648, 681, 835, 1017, 1144, 1216, 1318, 1521
LmnI	GCTCC	7 cut(s)	300, 341, 511, 1081, 1465, 1478, 1529
Lsp1109I	GCAGC	3 cut(s)	55, 257, 448
MaeI	CTAG	1 cut(s)	389
MaeII	ACGT	1 cut(s)	1425
MalI	GATC	9 cut(s)	105, 650, 683, 837, 1019, 1146, 1218, 1320, 1523
MboI	GATC	9 cut(s)	103, 648, 681, 835, 1017, 1144, 1216, 1318, 1521
MboII	GAAGA	4 cut(s)	124, 462, 738, 1164
MflI	RGATCY	2 cut(s)	835, 1318
MhlI	GDGCHC	5 cut(s)	297, 782, 1315, 1431, 1475
MluCI	AATT	5 cut(s)	75, 83, 183, 361, 620
MlyI	GAGTC	2 cut(s)	593, 1257
MmeI	TCCRAC	2 cut(s)	22, 133
Mph1103I	ATGCAT	2 cut(s)	255, 1039
MseI	TTAA	4 cut(s)	218, 440, 735, 743
MslI	CAYNNNNRTG	2 cut(s)	1038, 1295
MspCI	CTTAAG	2 cut(s)	734, 742
MspI	CCGG	1 cut(s)	381
MwoI	GCNNNNNNNGC	4 cut(s)	194, 308, 898, 1043
NdeI	CATATG	2 cut(s)	1039, 1486
NdeII	GATC	9 cut(s)	103, 648, 681, 835, 1017, 1144, 1216, 1318, 1521
NlaIII	CATG	8 cut(s)	102, 145, 257, 302, 704, 849, 1037, 1480
NlaIV	GGNNCC	4 cut(s)	196, 296, 806, 1474
NsiI	ATGCAT	2 cut(s)	255, 1039
NspI	RCATGY	2 cut(s)	849, 1037
PaeI	GCATGC	1 cut(s)	1037
PaeR7I	CTCGAG	1 cut(s)	1314
PceI	AGGCCT	1 cut(s)	740
PciI	ACATGT	1 cut(s)	845
PfeI	GAWTC	5 cut(s)	697, 907, 1109, 1129, 1400
PflMI	CCANNNNNTGG	1 cut(s)	935
PkrI	GCNGC	4 cut(s)	70, 247, 463, 1534
PleI	GAGTC	2 cut(s)	593, 1257
PpsI	GAGTC	2 cut(s)	593, 1257
Ppu21I	YACGTR	1 cut(s)	1426
PpuMI	RGGWCCY	2 cut(s)	305, 805
PscI	ACATGT	1 cut(s)	845
Psp124BI	GAGCTC	1 cut(s)	782
Psp5II	RGGWCCY	2 cut(s)	305, 805
PspFI	CCCAGC	1 cut(s)	267
PspN4I	GGNNCC	4 cut(s)	196, 296, 806, 1474
PspPI	GGNCC	4 cut(s)	305, 704, 805, 1451
PspPPI	RGGWCCY	2 cut(s)	305, 805
PstNI	CAGNNNCTG	1 cut(s)	308
PsuI	RGATCY	2 cut(s)	835, 1318
RsaI	GTAC	3 cut(s)	367, 1494, 1501
RsaNI	GTAC	3 cut(s)	366, 1493, 1500
RseI	CAYNNNNRTG	2 cut(s)	1038, 1295
SacI	GAGCTC	1 cut(s)	782
SaqAI	TTAA	4 cut(s)	218, 440, 735, 743
SatI	GCNGC	4 cut(s)	69, 246, 462, 1533
Sau3AI	GATC	9 cut(s)	103, 648, 681, 835, 1017, 1144, 1216, 1318, 1521
Sau96I	GGNCC	4 cut(s)	305, 704, 805, 1451
SchI	GAGTC	2 cut(s)	593, 1257
SduI	GDGCHC	5 cut(s)	297, 782, 1315, 1431, 1475
SfcI	CTRYAG	1 cut(s)	1056
Sfr274I	CTCGAG	1 cut(s)	1314
SinI	GGWCC	4 cut(s)	305, 704, 805, 1451
SlaI	CTCGAG	1 cut(s)	1314
SmiMI	CAYNNNNRTG	2 cut(s)	1038, 1295
SmlI	CTYRAG	9 cut(s)	60, 199, 556, 734, 742, 781, 1204, 1226, 1314
SmoI	CTYRAG	9 cut(s)	60, 199, 556, 734, 742, 781, 1204, 1226, 1314
SphI	GCATGC	1 cut(s)	1037
Sse9I	AATT	5 cut(s)	75, 83, 183, 361, 620
SseBI	AGGCCT	1 cut(s)	740
SsiI	CCGC	1 cut(s)	754
SspMI	CTAG	1 cut(s)	389
SstI	GAGCTC	1 cut(s)	782
StuI	AGGCCT	1 cut(s)	740
StyI	CCWWGG	3 cut(s)	481, 929, 1348
TaaI	ACNGT	3 cut(s)	151, 763, 968
TaiI	ACGT	1 cut(s)	1428
TaqI	TCGA	5 cut(s)	168, 651, 724, 1020, 1315
TasI	AATT	5 cut(s)	75, 83, 183, 361, 620
TatI	WGTACW	1 cut(s)	365
TfiI	GAWTC	5 cut(s)	697, 907, 1109, 1129, 1400
Tru1I	TTAA	4 cut(s)	218, 440, 735, 743
Tru9I	TTAA	4 cut(s)	218, 440, 735, 743
TscAI	CASTG	2 cut(s)	154, 973
TseI	GCWGC	4 cut(s)	68, 245, 461, 1532
TspDTI	ATGAA	5 cut(s)	27, 338, 689, 914, 1260
TspGWI	ACGGA	2 cut(s)	192, 1412
TspRI	CASTG	2 cut(s)	154, 973
Van91I	CCANNNNNTGG	1 cut(s)	935
Vha464I	CTTAAG	2 cut(s)	734, 742
VneI	GTGCAC	1 cut(s)	1427
VpaK11BI	GGWCC	4 cut(s)	305, 704, 805, 1451
XapI	RAATTY	2 cut(s)	75, 620
XbaI	TCTAGA	1 cut(s)	388
XceI	RCATGY	2 cut(s)	849, 1037
XcmI	CCANNNNNNNNNTGG	1 cut(s)	286
XhoI	CTCGAG	1 cut(s)	1314
XmiI	GTMKAC	1 cut(s)	6
XspI	CTAG	1 cut(s)	389
Zsp2I	ATGCAT	2 cut(s)	255, 1039

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.