Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0002932

Physical Location & Seq

Genomic Coordinates Forward (+)

8145998 .. 8152131

Gene Structure 6134 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G14140.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4560 bp

ATGGAAATGGAGACAAAAGCTGGAAAGCAAAAGAAGAGAAGTGTTGACTCGGATGATGATGTCTCTAAGGAAAGGAGACCAAAGCGAGCAGCAGCTTGCACAAACTTCAAGGAGAAATCTCTCCGTATCTCTGACAAATCTGAAACTGTTGAAGCTAAGAAAGAGCAGATCTTGGCGGAGGAGATCGTGGCCATACAGTTAACTTCTTCTTTGGAGAGCAATGATGATCCTCGTCCAAACCGGAGGCTGACTGATTTCGTTTTACATGATTCAGAAGGAGTTCCACAGCCTGTGGAGATGTTGGAACTTGGTGACATATTTATTGAAGGTGTTGTCTTACCTTTAGGTGATGAGAAAAAGGAAGAAAAGGGTGTCAGGTTTCAATCTTTTGGTCGAGTAGAGAACTGGAATATATCTGGTTATGAAGATGGTTCCCCGGTGATATGGATTTCAACGGCGTTAGCGGATTACGATTGCCGTAAACCTTCTAAGAAATACAAGAAATTATATGATTATTTCTTTGAGAAAGCTTGTGCTTGTGTGGAGGTGTTTAAGAGTTTGTCCAAGAATCCGGATACAAGTCTTGATGAGCTTCTTGCGGCGGTTTCTAGGTCGATGAGCGGAAGCAAGATCTTTTCTAGCGGTGGAGCCATCCAAGAGTTTGTTATATCCCAAGGAGAATTCATATATAACCAACTCGCTGGATTGGATGAGACAGCCAAGAATCATGAAACATGCTTTGTTGAGAATCGTGTTCTTGTTTCTCTAAGAGACCATGAGAGTAATAAAATTCACAAGGCTTTGTCTAATGTGGCTCTGAGGATTGATGAGAGCAAGGTCGTGACATCTGATCATCTGGTGGATGGTGCCGAGGACGAGGACGTAAAATATGCTAAGCTAATCCAAGAAGAAGAGTATCGGAAATCTATGGAGCGGTCGAGAAATAAGAGAAGCTCAACAACTTCTGGTGGTTCAAGCAGGTTTTACATTAAGATCAGTGAAGATGAGATTGCCGATGATTATCCTCTCCCGTCTTACTATAAGAACACCAAAGAAGAAACAGATGAGCTTGTTCTCTTTGAAGCTGGCTATGAGGTTGATACAAGAGACCTACCTTGCAGAACACTGCATAATTGGACTCTTTACAACTCTGATTCACGGATGATATCTTTAGAGGTTCTTCCCATGAGGCCGTGTGCTGAGATCGATGTCACCGTCTTTGGGTCAGGTGTTGTTGCTGAAGATGATGGAAGCGGGTTTTGTCTCGATGATTCGGAGAGCTCTACCTCTACGCAATCAAATGATCATGATGGGATGAATATATTCCTTAGTCAGATAAAGGAATGGATGATTGAGTTTGGGGCAGAAATGATCTTTGTCACATTACGAACTGACATGGCCTGGTACCGACTTGGGAAACCGTCAAAGCAATATGCTCCTTGGTTTGGAACTGTTATGAAAACAGTAAGGGTTGGCATAAGCATTTTCAATATGCTCATGAGGGAAAGTAGGGTTGCTAAGCTTTCATATGCAAATGTCATAAAAAGACTTTGTGGGTTAGAGGAGAACGATAAAGCTTACATTTCTTCTAAGCTCTTGGATGTTGAGAGATATGTTGTCGTCCATGGACAAATTATCTTGCAGCTTTTCGAAGAGTATCCTGACAAGGATATCAAAAGGTGTCCATTTGTTACTAGTCTTGCAAGTAAAATGCAGGATATACACCACACAAAATGGATCATCAAGAAGAAGAAGAAAATTCTGCAAAAGGGAAAGAATTTGAATCCGAGGGCGGGGATTGCACCTGTGGTATCCAGAATGAAAGCTATGCAAGCAACAACAACTCGCCTCGTCAATAGGATTTGGGGAGAGTTTTACTCCATTTACTCTCCTGAGGTTCCATCGGAGGCCATTAATGCTGAAAATGTGGAGGAAGAGGAGCTTGAAGAGGTAGAAGAGGAGGACGAGAATGAGGAAGATGATCCAGAGGAGAATGAACTAGAAGCTGTTGAGATTCAAAATTCTCCTACTCCTAAGAAAATTAAAGGTATTTCTGAAGATATGGAAATAAAATGGGATGGTGAGATTCTTGGCAAAACTTCTGCTGGTGAGCCTCTCTATGGAAGAGCCTTTGTTGGAGGGGATGTGGTGGTGGTAGGTAGTGCAGTCATCTTGGAAGTTGATGATCAAGATGATACTCAATTGATCTGTTTTGTGGAGTTCATGTTCGAGAGTTCAAACCACAGCAAGATGCTACATGGGAAACTCTTACAAAGAGGATCTGAGACTGTTCTAGGAATGGCTGCTAACGAGAGGGAACTGTTCTTGACTAATGAATGTCTTACTGTCCAGCTTAAGGACATAAAAGGAACAGTTAGTCTCGAGATTCGATCAAGGCTGTGGGGGCATCAATACAGGAAAGAGAACATCGATGTGGATAAGCTTGACCGGGCAAGAGCAGAAGAAAGAAAAACTAATGGTTTGCCAACAGACTACTACTGCAAAAGTTTGTACTCACCTGAGAGAGGTGGATTCTTTAGTCTTCCAAGAAATGATATGGGTCTAGGTTCTGGATTCTGTAGTTCATGTAAGATAAGAGAGAATGAAGAGGAAAGGTCAAAAACTAAACTCAATGATTCAAAGACAGGATTTCTCTCCAATGGGATAGAGTATCATAATGGAGATTTTGTCTATGTACTCCCCAACTACATAACTAAAGATGGATTGAAGAAGGGTAGTAGAAGAACAACTCTTAAGTGTGGTCGGAACGTTGGGTTAAAAGCTTTTGTTGTTTGCCAATTGCTGGATGTTATTGTTCTAGAAGAATCTAGAAAAGCTAGTAAGGCTTCATTTCAGGTTAAACTGACAAGGTTTTATAGGCCCGAGGACATTTCAGAAGAAAAGGCCTATGCTTCAGACATCCAAGAGTTGTATTATAGCCAGGATACATATATTCTCCCTCCGGAAGCTATACAGGGAAAATGTGAAGTAAGGAAGAAAAGTGATATGCCCCTATGTCGTGAGTATCCAATTTTAGACCATATCTTCTTCTGTGAAGTTTTCTATGATTCCTCTACTGGTTATCTCAAGCAGTTTCCGGCGAATATGAAGCTGAAGTTCTCTACTATTAAAGATGAAACACTTCTAAGAGAAAAGAAGGGGAAGGGAGTAGAAACTGGAACTAGTTCTGGAATGCTTATGAAGCCTGATGAGGTACCTAAAGAGAAGCCTCTAGCTACACTAGATATTTTTGCTGGATGTGGTGGTCTATCTCATGGACTAGAAAATGCTGGTGTATCTACTACAAAGTGGGCGATCGAGTATGAAGAGCCAGCTGGTCATGCGTTTAAACAAAACCATCCTGAAGCAACGGTTTTTGTTGACAACTGCAATGTGATCCTTAGGGCAATAATGGAGAAATGTGGAGATGTCGATGATTGTGTCTCTACTGTGGAGGCAGCTGAACTTGCAGCTAAACTTGATGAGAACCAAAAGAGTACCCTGCCACTTCCTGGTCAAGTAGATTTCATCAACGGAGGGCCTCCATGCCAGGGGTTTTCTGGTATGAATAGGTTCAGTCACGGCTCTTGGAGTAAAGTCCAGTGTGAAATGATATTAGCATTCTTGTCTTTTGCTGATTATTTCCGGCCAAAGTACTTTCTTCTCGAGAACGTGAAGAAATTTGTGACATACAATAAAGGGAGAACATTTCAACTTACTATGGCTTCTCTTCTTGAGATGGGTTACCAGGTAAGATTTGGAATCTTGGAGGCAGGTACATATGGAGTTTCCCAACCTCGTAAAAGAGTTATAATTTGGGCAGCTTCACCAGAAGAAGTTCTTCCAGAATGGCCTGAGCCGATGCATGTCTTTGATAATCCCGGTAGTAAAATCTCATTACCTCGAGGTTTACGCTATGATGCTGGTTGTAATACTAAATTTGGTGCACCTTTTCGTTCAATCACGGTGAGAGACACAATCGGCGATCTTCCACCAGTAGAAAACGGAGAATCCAAGATAAACAAAGAGTATGGAACTACTCCAGCCTCGTGGTTCCAAAAGAAGATAAGAGGAAACATGAGTGTTCTCACTGATCATATCTGCAAAGGGCTGAACGAACTAAACCTCATTCGATGTAAGAAAATCCCAAAGAGGCCTGGTGCTGATTGGCGTGACCTGCCGGACGAAAATGTGACATTATCAAATGGACTCGTGGAAAAATTGCGTCCTTTAGCTCTATCAAAGACAGCTAAAAACCACAACGAATGGAAGGGACTCTATGGTAGATTGGACTGGCAAGGAAACTTACCCATTTCCATCACCGATCCTCAGCCCATGGGTAAGGTGGGAATGTGCTTCCATCCTGAACAGGACAGAATAATCACTGTCCGTGAATGCGCCCGATCTCAGGGGTTTCCGGATAGCTACGAGTTTTCAGGGACGACAAAACACAAACATAGGCAAATTGGAAATGCAGTCCCTCCACCATTGGCCTTCGCTCTCGGTCGGAAGCTCAAAGAAGCCCTATATCTCAAGAGTTCTCTTCAACACCAATCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000278 GO:0000281 GO:0000910 GO:0003006 GO:0003674 GO:0003824 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006349 GO:0006725 GO:0006807 GO:0007049 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008356 GO:0009292 GO:0009294 GO:0009790 GO:0009791 GO:0009793 GO:0009909 GO:0009910 GO:0009987 GO:0010069 GO:0010070 GO:0010154 GO:0010216 GO:0010424 GO:0010468 GO:0016740 GO:0016741 GO:0019222 GO:0022402 GO:0022414 GO:0032259 GO:0032501 GO:0032502 GO:0032776 GO:0034641 GO:0040029 GO:0043170 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044728 GO:0044764 GO:0046483 GO:0048316 GO:0048519 GO:0048580 GO:0048581 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0051093 GO:0051239 GO:0051241 GO:0051301 GO:0051704 GO:0060255 GO:0061458 GO:0061640 GO:0065007 GO:0071514 GO:0071704 GO:0090304 GO:1901360 GO:1903047 GO:2000026 GO:2000241 GO:2000242

KEGG Pathways

Metabolic & Signaling

ko00270 ko01100 ko05206 map00270 map01100 map05206

Pfam Domains

Protein Families

BAH DNA_methylase DNMT1-RFD

Protein Analysis

1519

Amino Acids

171.58

Weight (kDa)

5.66

Isoelectric Point (pI)

42.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DNMT1-RFD	PF12047	76 - 205	3.9e-37	Cytosine specific DNA methyltransferase replication foci domain
DNMT1-RFD	PF12047	369 - 519	3.9e-44	Cytosine specific DNA methyltransferase replication foci domain
BAH	PF01426	717 - 849	9.5e-26	BAH domain
BAH	PF01426	889 - 1033	2.7e-28	BAH domain
DNA_methylase	PF00145	1079 - 1128	1e-06	C-5 cytosine-specific DNA methylase
DNA_methylase	PF00145	1163 - 1506	2e-43	C-5 cytosine-specific DNA methylase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0002932)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G08990 AT4G14140 AT4G14140 AT4G14140 AT5G49160 AT5G49160
fragaria_vesca	FvH4_1g10300 FvH4_1g10300
malus_domestica	MD02G1115100.v1.1 MD15G1231100.v1.1
prunus_persica	Prupe.7G060100_v2.0.a1 Prupe.7G183100_v2.0.a1 Prupe.7G183100_v2.0.a1
pyrus_communis	pycom02g09000 pycom02g09010 pycom15g20590 pycom15g20600
rosa_chinensis	RchiOBHm_Chr2g0097141 RchiOBHm_Chr5g0051581
rosa_laevigata	RLG00000016706 RLG00000034873
rosa_multiflora	Rmu_co8502141.1_g000001 Rmu_sc0001182.1_g000006 Rmu_ssc0000089.1_g000039
rosa_roxburghii	Rroxscaffold_1G00027090 Rroxscaffold_2G00144940
rosa_rugosa	Rorug02G0064600 Rorug05G0279000
rosa_samantha	Rh2AG111700 Rh2BG113700 Rh2CG116000 Rh2DG115000 Rh5AG350100 Rh5BG361900 Rh5CG386800 Rh5DG376800
rosa_wichuraiana	Rw2G008690 Rw5G033130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3812
AbsI	CCTCGAGG	1 cut(s)	3903
Acc36I	ACCTGC	3 cut(s)	969, 3764, 4185
Acc65I	GGTACC	2 cut(s)	1404, 3214
AccB1I	GGYRCC	3 cut(s)	866, 1404, 3214
AccB7I	CCANNNNNTGG	2 cut(s)	2803, 3512
AccBSI	CCGCTC	2 cut(s)	621, 934
AccIII	TCCGGA	3 cut(s)	571, 2962, 4417
AciI	CCGC	9 cut(s)	176, 464, 599, 602, 621, 642, 934, 1254, 1793
AclI	AACGTT	1 cut(s)	2769
AclWI	GGATC	6 cut(s)	221, 1745, 1976, 2287, 3391, 4319
AcoI	YGGCCR	2 cut(s)	189, 3647
AcsI	RAATTY	7 cut(s)	680, 789, 1758, 1777, 2020, 3680, 3938
AcuI	CTGAAG	5 cut(s)	1260, 2076, 2898, 3134, 3384
AfaI	GTAC	7 cut(s)	1406, 2513, 2697, 3216, 3499, 3656, 3778
AflII	CTTAAG	2 cut(s)	2354, 2753
AhlI	ACTAGT	2 cut(s)	1694, 3182
AjnI	CCWGG	6 cut(s)	1400, 2940, 3511, 3549, 3747, 4156
AloI	GAACNNNNNNTCC	2 cut(s)	2210, 2242
Alw21I	GWGCWC	2 cut(s)	1283, 3949
Alw44I	GTGCAC	1 cut(s)	3945
AlwI	GGATC	6 cut(s)	221, 1745, 1976, 2287, 3391, 4319
Ama87I	CYCGRG	4 cut(s)	2381, 2882, 3665, 3903
Aor13HI	TCCGGA	3 cut(s)	571, 2962, 4417
ApaLI	GTGCAC	1 cut(s)	3945
ApeKI	GCWGC	7 cut(s)	89, 92, 1642, 2303, 3458, 3470, 3821
ApoI	RAATTY	7 cut(s)	680, 789, 1758, 1777, 2020, 3680, 3938
AseI	ATTAAT	1 cut(s)	1914
Asp700I	GAANNNNTTC	7 cut(s)	279, 1322, 3141, 3184, 3572, 3837, 3840
Asp718I	GGTACC	2 cut(s)	1404, 3214
AspLEI	GCGC	1 cut(s)	4400
AspS9I	GGNCC	2 cut(s)	2880, 3539
AsuC2I	CCSGG	3 cut(s)	437, 2450, 3882
AsuII	TTCGAA	1 cut(s)	1650
AvaI	CYCGRG	4 cut(s)	2381, 2882, 3665, 3903
AxyI	CCTNAGG	2 cut(s)	1893, 3401
BaeGI	GKGCMC	1 cut(s)	3949
BalI	TGGCCA	1 cut(s)	191
BanI	GGYRCC	3 cut(s)	866, 1404, 3214
BanII	GRGCYC	1 cut(s)	1283
BauI	CACGAG	2 cut(s)	4048, 4211
BbsI	GAAGAC	1 cut(s)	2534
Bbv12I	GWGCWC	2 cut(s)	1283, 3949
BbvCI	CCTCAGC	1 cut(s)	4329
BbvI	GCAGC	7 cut(s)	101, 104, 1654, 2290, 3470, 3482, 3833
BceAI	ACGGC	4 cut(s)	462, 471, 1177, 3598
BciT130I	CCWGG	6 cut(s)	1402, 2942, 3513, 3551, 3749, 4158
BciVI	GTATCC	5 cut(s)	568, 1668, 1822, 2938, 3036
BclI	TGATCA	4 cut(s)	850, 1303, 2185, 4093
BcnI	CCSGG	3 cut(s)	437, 2450, 3882
BcuI	ACTAGT	2 cut(s)	1694, 3182
BfmI	CTRYAG	1 cut(s)	2578
BfrI	CTTAAG	2 cut(s)	2354, 2753
BfuAI	ACCTGC	3 cut(s)	969, 3764, 4185
BfuI	GTATCC	5 cut(s)	568, 1668, 1822, 2938, 3036
BglII	AGATCT	2 cut(s)	168, 630
BisI	GCNGC	8 cut(s)	90, 93, 600, 1643, 2304, 3459, 3471, 3822
BlpI	GCTNAGC	2 cut(s)	894, 1518
BlsI	GCNGC	8 cut(s)	91, 94, 601, 1644, 2305, 3460, 3472, 3823
BmcAI	AGTACT	1 cut(s)	3656
Bme1390I	CCNGG	9 cut(s)	437, 1402, 2450, 2942, 3513, 3551, 3749, 3882, 4158
BmeT110I	CYCGRG	4 cut(s)	2381, 2882, 3665, 3903
BmgT120I	GGNCC	2 cut(s)	2880, 3539
BmiI	GGNNCC	7 cut(s)	433, 649, 868, 1406, 1899, 3216, 4055
BmrFI	CCNGG	9 cut(s)	437, 1402, 2450, 2942, 3513, 3551, 3749, 3882, 4158
BmsI	GCATC	4 cut(s)	2241, 2416, 3852, 3910
BpiI	GAAGAC	1 cut(s)	2534
BplI	GAGNNNNNCTC	2 cut(s)	1862, 1894
BpmI	CTGGAG	1 cut(s)	4026
Bpu10I	CCTNAGC	2 cut(s)	3855, 4329
Bpu1102I	GCTNAGC	2 cut(s)	894, 1518
Bpu14I	TTCGAA	1 cut(s)	1650
BpuEI	CTTGAG	3 cut(s)	3071, 3755, 4517
BpuMI	CCSGG	3 cut(s)	437, 2450, 3882
Bsa29I	ATCGAT	2 cut(s)	1206, 2430
BsaBI	GATNNNNATC	1 cut(s)	1020
BsaI	GGTCTC	3 cut(s)	70, 765, 1101
BsaJI	CCNNGG	9 cut(s)	435, 673, 870, 1439, 1624, 1787, 2883, 3550, 4335
BsaWI	WCCGGW	4 cut(s)	240, 571, 2962, 4417
BsaXI	ACNNNNNCTCC	6 cut(s)	2130, 2160, 2210, 2240, 2673, 2703
Bse1I	ACTGG	6 cut(s)	410, 3082, 3181, 3601, 3995, 4298
Bse21I	CCTNAGG	2 cut(s)	1893, 3401
Bse3DI	GCAATG	2 cut(s)	226, 3397
Bse8I	GATNNNNATC	1 cut(s)	1020
BseAI	TCCGGA	3 cut(s)	571, 2962, 4417
BseBI	CCWGG	6 cut(s)	1402, 2942, 3513, 3551, 3749, 4158
BseCI	ATCGAT	2 cut(s)	1206, 2430
BseDI	CCNNGG	9 cut(s)	435, 673, 870, 1439, 1624, 1787, 2883, 3550, 4335
BseJI	GATNNNNATC	1 cut(s)	1020
BseMI	GCAATG	2 cut(s)	226, 3397
BseMII	CTCAG	8 cut(s)	809, 1191, 1884, 2274, 2511, 3846, 4343, 4421
BseNI	ACTGG	6 cut(s)	410, 3082, 3181, 3601, 3995, 4298
BseRI	GAGGAG	5 cut(s)	194, 1577, 1952, 1973, 2003
BseSI	GKGCMC	1 cut(s)	3949
BseXI	GCAGC	7 cut(s)	101, 104, 1654, 2290, 3470, 3482, 3833
BsgI	GTGCAG	1 cut(s)	2184
Bsh1285I	CGRYCG	3 cut(s)	938, 3318, 4507
BshNI	GGYRCC	3 cut(s)	866, 1404, 3214
BshVI	ATCGAT	2 cut(s)	1206, 2430
BsiEI	CGRYCG	3 cut(s)	938, 3318, 4507
BsiHKAI	GWGCWC	2 cut(s)	1283, 3949
BsiHKCI	CYCGRG	4 cut(s)	2381, 2882, 3665, 3903
BslFI	GGGAC	3 cut(s)	4287, 4453, 4463
BsmFI	GGGAC	3 cut(s)	4287, 4453, 4463
BsmI	GAATGC	3 cut(s)	3198, 3620, 4400
Bso31I	GGTCTC	3 cut(s)	70, 765, 1101
BsoBI	CYCGRG	4 cut(s)	2381, 2882, 3665, 3903
Bsp119I	TTCGAA	1 cut(s)	1650
Bsp1286I	GDGCHC	2 cut(s)	1283, 3949
Bsp13I	TCCGGA	3 cut(s)	571, 2962, 4417
Bsp1720I	GCTNAGC	2 cut(s)	894, 1518
Bsp19I	CCATGG	2 cut(s)	1624, 4335
BspACI	CCGC	9 cut(s)	176, 464, 599, 602, 621, 642, 934, 1254, 1793
BspCNI	CTCAG	8 cut(s)	810, 1192, 1885, 2275, 2512, 3847, 4342, 4420
BspDI	ATCGAT	2 cut(s)	1206, 2430
BspEI	TCCGGA	3 cut(s)	571, 2962, 4417
BspHI	TCATGA	3 cut(s)	727, 1306, 1497
BspLI	GGNNCC	7 cut(s)	433, 649, 868, 1406, 1899, 3216, 4055
BspMI	ACCTGC	3 cut(s)	969, 3764, 4185
BspPI	GGATC	6 cut(s)	221, 1745, 1976, 2287, 3391, 4319
BspQI	GCTCTTC	2 cut(s)	2119, 3321
BspT104I	TTCGAA	1 cut(s)	1650
BspT107I	GGYRCC	3 cut(s)	866, 1404, 3214
BspTI	CTTAAG	2 cut(s)	2354, 2753
BspTNI	GGTCTC	3 cut(s)	70, 765, 1101
BsrBI	CCGCTC	2 cut(s)	621, 934
BsrDI	GCAATG	2 cut(s)	226, 3397
BsrI	ACTGG	6 cut(s)	410, 3082, 3181, 3601, 3995, 4298
BssECI	CCNNGG	9 cut(s)	435, 673, 870, 1439, 1624, 1787, 2883, 3550, 4335
BssSI	CACGAG	2 cut(s)	4048, 4211
BssT1I	CCWWGG	4 cut(s)	673, 1439, 1624, 4335
Bst2BI	CACGAG	2 cut(s)	4048, 4211
Bst2UI	CCWGG	6 cut(s)	1402, 2942, 3513, 3551, 3749, 4158
BstAFI	CTTAAG	2 cut(s)	2354, 2753
BstBI	TTCGAA	1 cut(s)	1650
BstC8I	GCNNGC	5 cut(s)	87, 97, 1087, 1833, 3333
BstDSI	CCRYGG	2 cut(s)	1624, 4335
BstEII	GGTNACC	1 cut(s)	3743
BstENI	CCTNNNNNAGG	1 cut(s)	2523
BstHHI	GCGC	1 cut(s)	4400
BstMCI	CGRYCG	3 cut(s)	938, 3318, 4507
BstMWI	GCNNNNNNNGC	9 cut(s)	1799, 1832, 1916, 2404, 2843, 3202, 3341, 3467, 4177
BstNI	CCWGG	6 cut(s)	1402, 2942, 3513, 3551, 3749, 4158
BstNSI	RCATGY	2 cut(s)	738, 3869
BstPI	GGTNACC	1 cut(s)	3743
BstSCI	CCNGG	9 cut(s)	435, 1400, 2448, 2940, 3511, 3549, 3747, 3880, 4156
BstSFI	CTRYAG	1 cut(s)	2578
BstSLI	GKGCMC	1 cut(s)	3949
BstV1I	GCAGC	7 cut(s)	101, 104, 1654, 2290, 3470, 3482, 3833
BstV2I	GAAGAC	1 cut(s)	2534
BstX2I	RGATCY	3 cut(s)	168, 630, 2279
BstXI	CCANNNNNNTGG	2 cut(s)	701, 4050
BstYI	RGATCY	3 cut(s)	168, 630, 2279
Bsu15I	ATCGAT	2 cut(s)	1206, 2430
Bsu36I	CCTNAGG	2 cut(s)	1893, 3401
BsuI	GTATCC	5 cut(s)	568, 1668, 1822, 2938, 3036
BsuTUI	ATCGAT	2 cut(s)	1206, 2430
BtgI	CCRYGG	2 cut(s)	1624, 4335
BtsI	GCAGTG	1 cut(s)	1124
BtsIMutI	CAGTG	5 cut(s)	1003, 1124, 3608, 4089, 4383
BveI	ACCTGC	3 cut(s)	969, 3764, 4185
Cac8I	GCNNGC	5 cut(s)	87, 97, 1087, 1833, 3333
CciI	TCATGA	3 cut(s)	727, 1306, 1497
CfoI	GCGC	1 cut(s)	4400
Cfr13I	GGNCC	2 cut(s)	2880, 3539
ClaI	ATCGAT	2 cut(s)	1206, 2430
CseI	GACGC	1 cut(s)	4214
CsiI	ACCWGGT	1 cut(s)	3747
Csp6I	GTAC	7 cut(s)	1405, 2512, 2696, 3215, 3498, 3655, 3777
CviQI	GTAC	7 cut(s)	1405, 2512, 2696, 3215, 3498, 3655, 3777
DraI	TTTAAA	1 cut(s)	3349
EaeI	YGGCCR	2 cut(s)	189, 3647
EciI	GGCGGA	1 cut(s)	191
Ecl136II	GAGCTC	1 cut(s)	1281
Eco130I	CCWWGG	4 cut(s)	673, 1439, 1624, 4335
Eco147I	AGGCCT	2 cut(s)	2906, 4156
Eco24I	GRGCYC	1 cut(s)	1283
Eco31I	GGTCTC	3 cut(s)	70, 765, 1101
Eco32I	GATATC	2 cut(s)	1167, 1672
Eco53kI	GAGCTC	1 cut(s)	1281
Eco57I	CTGAAG	5 cut(s)	1260, 2076, 2898, 3134, 3384
Eco81I	CCTNAGG	2 cut(s)	1893, 3401
Eco88I	CYCGRG	4 cut(s)	2381, 2882, 3665, 3903
Eco91I	GGTNACC	1 cut(s)	3743
EcoICRI	GAGCTC	1 cut(s)	1281
EcoNI	CCTNNNNNAGG	1 cut(s)	2523
EcoO109I	RGGNCCY	1 cut(s)	3539
EcoO65I	GGTNACC	1 cut(s)	3743
EcoRI	GAATTC	1 cut(s)	680
EcoRII	CCWGG	6 cut(s)	1400, 2940, 3511, 3549, 3747, 4156
EcoRV	GATATC	2 cut(s)	1167, 1672
EcoT14I	CCWWGG	4 cut(s)	673, 1439, 1624, 4335
EcoT22I	ATGCAT	1 cut(s)	3867
EcoT38I	GRGCYC	1 cut(s)	1283
ErhI	CCWWGG	4 cut(s)	673, 1439, 1624, 4335
FalI	AAGNNNNNCTT	4 cut(s)	1926, 1958, 3825, 3857
FaqI	GGGAC	3 cut(s)	4287, 4453, 4463
FauI	CCCGC	2 cut(s)	1247, 1786
FauNDI	CATATG	2 cut(s)	1528, 3781
FbaI	TGATCA	4 cut(s)	850, 1303, 2185, 4093
Fnu4HI	GCNGC	8 cut(s)	90, 93, 600, 1643, 2304, 3459, 3471, 3822
FriOI	GRGCYC	1 cut(s)	1283
Fsp4HI	GCNGC	8 cut(s)	90, 93, 600, 1643, 2304, 3459, 3471, 3822
GlaI	GCGC	1 cut(s)	4399
GluI	GCNGC	8 cut(s)	90, 93, 600, 1643, 2304, 3459, 3471, 3822
GsuI	CTGGAG	1 cut(s)	4026
HgaI	GACGC	1 cut(s)	4214
HhaI	GCGC	1 cut(s)	4400
Hin6I	GCGC	1 cut(s)	4398
HinP1I	GCGC	1 cut(s)	4398
HincII	GTYRAC	3 cut(s)	46, 201, 3382
HindII	GTYRAC	3 cut(s)	46, 201, 3382
HindIII	AAGCTT	5 cut(s)	528, 1520, 1575, 2441, 2781
HpaI	GTTAAC	1 cut(s)	201
Hpy166II	GTNNAC	6 cut(s)	46, 201, 482, 3382, 3911, 3947
Hpy8I	GTNNAC	6 cut(s)	46, 201, 482, 3382, 3911, 3947
HpyAV	CCTTC	8 cut(s)	269, 320, 495, 2725, 3151, 3157, 4264, 4504
HpyCH4IV	ACGT	3 cut(s)	882, 2769, 3672
HpyF10VI	GCNNNNNNNGC	9 cut(s)	1799, 1832, 1916, 2404, 2843, 3202, 3341, 3467, 4177
HpySE526I	ACGT	3 cut(s)	882, 2769, 3672
HspAI	GCGC	1 cut(s)	4398
Kpn2I	TCCGGA	3 cut(s)	571, 2962, 4417
KpnI	GGTACC	2 cut(s)	1408, 3218
Ksp22I	TGATCA	4 cut(s)	850, 1303, 2185, 4093
KspAI	GTTAAC	1 cut(s)	201
LguI	GCTCTTC	2 cut(s)	2119, 3321
LmnI	GCTCC	4 cut(s)	647, 931, 1441, 1939
Lsp1109I	GCAGC	7 cut(s)	101, 104, 1654, 2290, 3470, 3482, 3833
LweI	GCATC	4 cut(s)	2241, 2416, 3852, 3910
MabI	ACCWGGT	1 cut(s)	3747
MaeII	ACGT	3 cut(s)	882, 2769, 3672
MbiI	CCGCTC	2 cut(s)	621, 934
MfeI	CAATTG	2 cut(s)	2201, 2798
MflI	RGATCY	3 cut(s)	168, 630, 2279
MhlI	GDGCHC	2 cut(s)	1283, 3949
MlsI	TGGCCA	1 cut(s)	191
MluNI	TGGCCA	1 cut(s)	191
MlyI	GAGTC	4 cut(s)	41, 1132, 4203, 4269
MmeI	TCCRAC	4 cut(s)	282, 2116, 2744, 4487
Mox20I	TGGCCA	1 cut(s)	191
Mph1103I	ATGCAT	1 cut(s)	3867
MroI	TCCGGA	3 cut(s)	571, 2962, 4417
MroXI	GAANNNNTTC	7 cut(s)	279, 1322, 3141, 3184, 3572, 3837, 3840
MscI	TGGCCA	1 cut(s)	191
MslI	CAYNNNNRTG	1 cut(s)	2432
Msp20I	TGGCCA	1 cut(s)	191
MspA1I	CMGCKG	2 cut(s)	3335, 3461
MspCI	CTTAAG	2 cut(s)	2354, 2753
MspR9I	CCNGG	9 cut(s)	437, 1402, 2450, 2942, 3513, 3551, 3749, 3882, 4158
MssI	GTTTAAAC	1 cut(s)	3349
MunI	CAATTG	2 cut(s)	2201, 2798
Mva1269I	GAATGC	3 cut(s)	3198, 3620, 4400
MvaI	CCWGG	6 cut(s)	1402, 2942, 3513, 3551, 3749, 4158
MwoI	GCNNNNNNNGC	9 cut(s)	1799, 1832, 1916, 2404, 2843, 3202, 3341, 3467, 4177
NciI	CCSGG	3 cut(s)	437, 2450, 3882
NcoI	CCATGG	2 cut(s)	1624, 4335
NdeI	CATATG	2 cut(s)	1528, 3781
NlaIV	GGNNCC	7 cut(s)	433, 649, 868, 1406, 1899, 3216, 4055
NmeAIII	GCCGAG	1 cut(s)	895
NmuCI	GTSAC	8 cut(s)	311, 841, 1210, 1378, 3578, 3685, 4172, 4192
NsiI	ATGCAT	1 cut(s)	3867
NspI	RCATGY	2 cut(s)	738, 3869
NspV	TTCGAA	1 cut(s)	1650
PaeR7I	CTCGAG	3 cut(s)	2381, 3665, 3903
PagI	TCATGA	3 cut(s)	727, 1306, 1497
PceI	AGGCCT	2 cut(s)	2906, 4156
PciSI	GCTCTTC	2 cut(s)	2119, 3321
PcsI	WCGNNNNNNNCGW	1 cut(s)	1212
PctI	GAATGC	3 cut(s)	3198, 3620, 4400
PdmI	GAANNNNTTC	7 cut(s)	279, 1322, 3141, 3184, 3572, 3837, 3840
PflMI	CCANNNNNTGG	2 cut(s)	2803, 3512
PkrI	GCNGC	8 cut(s)	91, 94, 601, 1644, 2305, 3460, 3472, 3823
Ple19I	CGATCG	1 cut(s)	3318
PleI	GAGTC	4 cut(s)	41, 1132, 4203, 4269
PmeI	GTTTAAAC	1 cut(s)	3349
PpsI	GAGTC	4 cut(s)	41, 1132, 4203, 4269
PshBI	ATTAAT	1 cut(s)	1914
PsiI	TTATAA	1 cut(s)	3812
Psp124BI	GAGCTC	1 cut(s)	1283
Psp1406I	AACGTT	1 cut(s)	2769
Psp6I	CCWGG	6 cut(s)	1400, 2940, 3511, 3549, 3747, 4156
PspEI	GGTNACC	1 cut(s)	3743
PspGI	CCWGG	6 cut(s)	1400, 2940, 3511, 3549, 3747, 4156
PspN4I	GGNNCC	7 cut(s)	433, 649, 868, 1406, 1899, 3216, 4055
PspPI	GGNCC	2 cut(s)	2880, 3539
PspXI	VCTCGAGB	1 cut(s)	3903
PsuI	RGATCY	3 cut(s)	168, 630, 2279
PvuI	CGATCG	1 cut(s)	3318
PvuII	CAGCTG	2 cut(s)	3335, 3461
RsaI	GTAC	7 cut(s)	1406, 2513, 2697, 3216, 3499, 3656, 3778
RsaNI	GTAC	7 cut(s)	1405, 2512, 2696, 3215, 3498, 3655, 3777
RseI	CAYNNNNRTG	1 cut(s)	2432
SacI	GAGCTC	1 cut(s)	1283
SapI	GCTCTTC	2 cut(s)	2119, 3321
SatI	GCNGC	8 cut(s)	90, 93, 600, 1643, 2304, 3459, 3471, 3822
Sau96I	GGNCC	2 cut(s)	2880, 3539
ScaI	AGTACT	1 cut(s)	3656
SchI	GAGTC	4 cut(s)	41, 1132, 4203, 4269
ScrFI	CCNGG	9 cut(s)	437, 1402, 2450, 2942, 3513, 3551, 3749, 3882, 4158
SduI	GDGCHC	2 cut(s)	1283, 3949
SexAI	ACCWGGT	1 cut(s)	3747
SfaNI	GCATC	4 cut(s)	2241, 2416, 3852, 3910
SfcI	CTRYAG	1 cut(s)	2578
Sfr274I	CTCGAG	3 cut(s)	2381, 3665, 3903
SfuI	TTCGAA	1 cut(s)	1650
SlaI	CTCGAG	3 cut(s)	2381, 3665, 3903
SmiMI	CAYNNNNRTG	1 cut(s)	2432
SmlI	CTYRAG	8 cut(s)	2354, 2381, 2753, 3086, 3665, 3734, 3903, 4532
SmoI	CTYRAG	8 cut(s)	2354, 2381, 2753, 3086, 3665, 3734, 3903, 4532
SpeI	ACTAGT	2 cut(s)	1694, 3182
SseBI	AGGCCT	2 cut(s)	2906, 4156
SsiI	CCGC	9 cut(s)	176, 464, 599, 602, 621, 642, 934, 1254, 1793
SstI	GAGCTC	1 cut(s)	1283
StuI	AGGCCT	2 cut(s)	2906, 4156
StyD4I	CCNGG	9 cut(s)	435, 1400, 2448, 2940, 3511, 3549, 3747, 3880, 4156
StyI	CCWWGG	4 cut(s)	673, 1439, 1624, 4335
TaiI	ACGT	3 cut(s)	885, 2772, 3675
TaqII	GACCGA	1 cut(s)	4493
TatI	WGTACW	3 cut(s)	2511, 2695, 3654
TauI	GCSGC	1 cut(s)	602
TscAI	CASTG	5 cut(s)	1003, 1131, 3608, 4096, 4390
TseFI	GTSAC	8 cut(s)	311, 841, 1210, 1378, 3578, 3685, 4172, 4192
TseI	GCWGC	7 cut(s)	89, 92, 1642, 2303, 3458, 3470, 3821
Tsp45I	GTSAC	8 cut(s)	311, 841, 1210, 1378, 3578, 3685, 4172, 4192
TspGWI	ACGGA	5 cut(s)	113, 1174, 3549, 4020, 4379
TspRI	CASTG	5 cut(s)	1003, 1131, 3608, 4096, 4390
Van91I	CCANNNNNTGG	2 cut(s)	2803, 3512
Vha464I	CTTAAG	2 cut(s)	2354, 2753
VneI	GTGCAC	1 cut(s)	3945
VspI	ATTAAT	1 cut(s)	1914
XagI	CCTNNNNNAGG	1 cut(s)	2523
XapI	RAATTY	7 cut(s)	680, 789, 1758, 1777, 2020, 3680, 3938
XbaI	TCTAGA	2 cut(s)	2818, 2828
XceI	RCATGY	2 cut(s)	738, 3869
XcmI	CCANNNNNNNNNTGG	3 cut(s)	3557, 3755, 4342
XhoI	CTCGAG	3 cut(s)	2381, 3665, 3903
XmnI	GAANNNNTTC	7 cut(s)	279, 1322, 3141, 3184, 3572, 3837, 3840
ZrmI	AGTACT	1 cut(s)	3656
Zsp2I	ATGCAT	1 cut(s)	3867

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.