Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5D

Orthogroup ID

OG0002932

Physical Location & Seq

Genomic Coordinates Reverse (-)

57379713 .. 57385883

Gene Structure 6171 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5DG376800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4707 bp

ATGGGATCCGCCGCAGGATTAGAACCCGACCCTCCCTCCAAAGTGACTAAACCAACATCTTCTTCGTCTTCCTCTGGAATGACAAAGAAGAAGGGCAAAAAAGCTTCTCAAAAGGAAGCACCTACTGGCCGTAGAGGTAGGAAGCGAAATTTGCCTCAGAGCGATGAAGAGCATGCCCAGTCTCGCAAAATGCCCAAGCGTGCTGCTGCATGTAAAGACTTTAAGGACAAGTCTGGCCATATTACTGATGAGTCTGATCTCATCGCAAGCAAGGAGGAACAGATTGTGCCAGAAGAAGGTCTTGCTGTACGCTTGACTTGTGGCAAGGACCCAGATAATGAGAAGCCAAGCAGAAGACTGACTCAATTTATCTTGCATAGTGAAGATGGTACTGCAAGACCACTTGAGATGGTGGAAGCTGGTCTGTTTATAACTGGCACTATATTGCCTCTTCGTGAAAGTACTGAGAAGGACAAGGACAAGGACAAGGACAGCAGTATTAAATGTGAAGGTTTTGGGCGAATCGATGCTTGGAAGATCTCTGGTTACGATGAGGGCTCTCCAATGATATGGCTTTCAACTAAAGTTGCCGATTATTGTTGTGTTAAACCAGCAATTGGCTACAAGAAGCACTATGATCTCTTCTTTGAGAAAGCGCGTGCTTGCATAGAGGTGTACCAGAAACTTTCAAAATGTAAATCTGGCTGTAGTCTTGAAGAATTACTTGCTGGAGTTGCTCGATCATTGAGCGGAAGCAAATTCTTTTCTGGCAGTGCATCTATCAAAGAATTTGTCCTATCTCACGGTGAGTTTATCTATAAACGATTGATAGGTTTGGAGCAGTCATTGAAGAAGAATGATCAGATATTGGCACAGCTGCCTGTTCTCACTGCCCTCAGAGATGAGTCTATAAACCATGGATATTATGAGCAATCAGAACCAGTAGCTTCAAGTGCTACCTTAAAGATTGCTTCAGATATCAGCGTTGGAGGGAATGAACTGAGAACAACCGGTTCATCCATAGTTGAAGCTGAAGAAGATGAAGATGCAAAGTTAGCTAGACTGCTGCAAGAAGAAGAATGCTTTAAGTCTATGAAACAAAGAAAACGTCAGGGTGCTACCTCCACGTCGAGTAAATTCTACATCAAAATTAATGAAGATGAAATTGCTAATGATTATCCTGAACCTAAATATTATGACAATTCCATTGAAGAGACGGATGAGTTTATTGTTGTTGACAGTGAGGCTGGTGATCTTCCCACACATGTGCTTGATAATTGGTGTCTGTACAACTTAGACTCGAGACTGATTTCGTTGGAGCTTCTTCCAATGACAGATTGCCCAGATATTGATGTTACAATTTTTGGGTCAGGGGTTATGAGTGAAGATGATGGAAGCTGTGGCTTTAATCTTGATGCTGACAGTATCCAGTCTTCTTCAAGTGGTTCAGGGGCACCAGTAGCAGAAGATGCTTATGGCATCCCAATTTATTTGAGTGCCATAAAGGAATGGGTGATAGAATGGGGAGCATCAATGATCTCAGTATCAATTCGAACAGAGCTGGCCTGGTACAGACTTGGCAAGCCATCGCAGCAGTATGCTTTGTGGTATGAACCAATACTGAAAACAGCAAGGGTTGGGAGAAGTATAATCACTATGCTCAAGGCTGAACGTCGAGTAGCACGGCTTTCTTTTGCAGATGTCATTAAACGATTGTCAGAGTTCCCAAAGAACCATGGTGGTTACATTTCTTCTGATCCAGCATCTGTTGAGAGATATGTGGTTGTACATGGAGCGATAATATTGCAGCTGTTTTCACAGTTTCCAGATGTGAAGATTAAAAAATGTTCATTTCTTGATGGTCTTCGAGACAAAATGGAGAAGAGGCACCATACTAAATGGGAAGTAAAGAGCAAGAAAATTTTGCAGAAGAGTGAATCAAATTTGAACCCACGGGCATCAATGGGACCTGTGGTTTCCAAGAGGAAGGCAATGCAGGCTACAACAACAAAGCTTATCAACAGAATTTGGGGGGACTACTATTCAAATTACTCTCCAGTAGATTCAAGCCCAGGAGCTACTTGTGGAAAGGTAGAGGAGAATGAAGTTATAGAAGAGGTCCAAGAGGACATAGAAGAGTATGATGTAGATGAGAATTCATCAGTAATGGAACAAGCCCAAAGGTCTCCTGCAGTTTCAAGAAAGCCTAAAGCATGCTTCAGCAGCAGTGAGATTTTGTGGGGTGAGGAGCCTGTGGGAAAAACATGTTCTGGTAAAGCTCTCTACAAGCATGCCGTTCTTCGTGGAGATGAGATCTCTGTTGGTGGTGCTGTTTTTGTGGAAATTGATGTATCAAAAGATCCTGTTATCTATCTTGTTGAGTACATGTTTGAGACGTTAGATGGAAAGAAGATGTTCCATGGGAGAATGATGCAGAGAGGATCTGAGACTGTTCTTGGAAACACTGCTAATGAGAGAGAAGTGTTTTTAACTGATGAGTGCGTGAACTTGGCATTGGAGGATGTGGAACAGACTGTTGTTGTGGGTATCCAATCAATGCCATGGGGGCATCAGTATAGGAAGAAGAATGCCGATACTAATAGAATTGATAGAGCAAGAGCAGAAGAGAGGAAGAAGAAAGGATTGCCAACAGAATATTACTGTAAAAGCCGGTATTGCCCTGAGAAAGGTGCTTTCTTTAGTCTTTCTCATCATACTATCGGTCTGGGTTCTGGTTTTTGCCACTCTTGCAAAGTTAATGAAGATGAAAAGGCTATGGAAGTCTTTAAAGTGAATTCATCAAAGACTGGTTTTGTATACAGTGGAGCTGAGTATTTCATTCATGATTATGTCTATGTAAGCCCCTATCATTTTTCTGTGGAAAGGATTGGGACTGAGCTTTTCAAGGCTGGGAGGAATCTGGGATTGAAAGCTTATGTTGTGTGCCAAGTGCTGGACATAATTACTGCAAAGGAATATAAACAACCTGATATGAAGTCTACCCAGGTTAAAGTTCGAAGGTTTTTCAGACCAGAGGACATTTCAGTAGAGAAGGCCTACTGCTCTGATGTCAGAGAGGTCTACTACAGTGAAGAAACACACATTATCCCTGCTGATAGCTTAGAAGGAAAATGTGAAGTTAGAAAGAGGAGTGATCTTCCAGAATGTGATGCTCCTGCAATGTTTGAGCATATTTTCTTTTGTGAGCATTTATATGATCCTTCTAAAGGCTCTCTTAAGCAGTTACCGGTTCACATCAAGGTGAGGCACTCAGCAGTTGGTGGTGATGCTGAATCTAGAAAGAAAAAGGGCAAATGCAAAGAAGGAGAAGATGTTCCAGAAGTTGAGAAACAGAGATATGATTCTGAACACAAACGCCTAGCCACTTTGGATATATTTGCTGGATGTGGTGGCCTGTCAGAAGGTTTGCATCAGGCTGGTGTTTCAGTAACGAAGTGGGCAATCGAGTATGAAGAGCCGGCTGGACAGGCTTTCCAACTCAACCATCCTGAGTCCAAGGTGTTCATTAATAATTGCAATGTCATCTTGAAGGCCGTCATGGACAAATGTGGAGATATGGTTGATTGCATCTCCACTACTGAAGCCAATGATTTAGCCTCGTCACTTGATGAGAAGGTGAAAGACAATTTGCCCTTGCCAGGGCAAGTAGACTTTATTAATGGAGGCCCTCCATGCCAGGGATTTTCTGGTATGAATAGATTCAATACCAGCACTTGGAGTAAAGTCCAGTGTGAAATGATATTGGCATTCTTATCCTTTGCTGATTACTTCCGGCCAAAATACTTCCTCCTGGAGAATGTGAGGAACTTTGTGGCTTTTAATAAAGGACAGACATTCCGTCTGACTGTGGCTTCACTTCTTGAGATGGGTTATCAGGTGAGGTTTGGTGTTCTGGAAGCTGGAGCTTATGGTGTTTCTCAATCCCGAAAGCGTGCATTTATCTGGGCAGCCGCACCAGAAGAGGTTCTTCCAGATTGGCCAGAGCCAATGCATGTTTTTGGTGTGCCAGAGTTGAAGATCAATTTATCCGGGAACTCCTATTATGCTGCTGTTCGAAGTACTGCAACCGGTGCCCCATTCCGTGCCATAACAGTAAGAGACACAATTGGCGATCTTCCTGCTGTAGGCAATGGAGCTTCCAAGATCAATTTGGAATATGAAAGCGACCCTGTCTCGTGGTTCCAGAAGAGAATCAGAGGGAACATGGCTGTTTTGACTGATCACATTTCGAAAGAAATGAACGAGCTGAACCTCATCCGATGTAAGAAGATTCCCAAGAGGCCAGGTGCAGATTGGCAGGACCTTCCTGAGGAAAAGGTGACGCTCTCTACTGGTCAAATAGTGGACTTGATACCATGGTGCTTGCCCAACACAGCGAAACGCCACAACCAGTGGAAGGGCCTGTTTGGAAGGTTGGACTGGGAAGGCAACTTCCCAACCTCCATCACAGATCCTCAGCCAATGGGTAAGGTGGGAATGTGCTTTCATCCTGACCAGGACAGAATTCTGACAGTTAGGGAATGTGCTCGATCTCAAGGATTTCCTGACAGCTACCAGTTTTACGGCAACATTCTTCACAAGCATAGGCAGATTGGAAATGCTGTTCCTCCGACTCTGGCTTATGCTCTGGGCAGAAAACTCAAGGAAGCAGTGGATAGCAAGAAGAAGTCTTCTTCACAAGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000278 GO:0000281 GO:0000910 GO:0003006 GO:0003674 GO:0003824 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006349 GO:0006725 GO:0006807 GO:0007049 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008356 GO:0009292 GO:0009294 GO:0009790 GO:0009791 GO:0009793 GO:0009909 GO:0009910 GO:0009987 GO:0010069 GO:0010070 GO:0010154 GO:0010216 GO:0010424 GO:0010468 GO:0016740 GO:0016741 GO:0019222 GO:0022402 GO:0022414 GO:0032259 GO:0032501 GO:0032502 GO:0032776 GO:0034641 GO:0040029 GO:0043170 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044728 GO:0044764 GO:0046483 GO:0048316 GO:0048519 GO:0048580 GO:0048581 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0051093 GO:0051239 GO:0051241 GO:0051301 GO:0051704 GO:0060255 GO:0061458 GO:0061640 GO:0065007 GO:0071514 GO:0071704 GO:0090304 GO:1901360 GO:1903047 GO:2000026 GO:2000241 GO:2000242

KEGG Pathways

Metabolic & Signaling

ko00270 ko01100 ko05206 map00270 map01100 map05206

Pfam Domains

Protein Families

BAH DNA_methylase DNMT1-RFD

Protein Analysis

1568

Amino Acids

175.37

Weight (kDa)

6.29

Isoelectric Point (pI)

46.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DNMT1-RFD	PF12047	113 - 247	3.7e-31	Cytosine specific DNA methyltransferase replication foci domain
DNMT1-RFD	PF12047	418 - 574	3.4e-31	Cytosine specific DNA methyltransferase replication foci domain
BAH	PF01426	771 - 902	2.8e-18	BAH domain
BAH	PF01426	945 - 1083	7.1e-25	BAH domain
DNA_methylase	PF00145	1128 - 1555	1.8e-47	C-5 cytosine-specific DNA methylase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002932)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G08990 AT4G14140 AT4G14140 AT4G14140 AT5G49160 AT5G49160
fragaria_vesca	FvH4_1g10300 FvH4_1g10300
malus_domestica	MD02G1115100.v1.1 MD15G1231100.v1.1
prunus_persica	Prupe.7G060100_v2.0.a1 Prupe.7G183100_v2.0.a1 Prupe.7G183100_v2.0.a1
pyrus_communis	pycom02g09000 pycom02g09010 pycom15g20590 pycom15g20600
rosa_chinensis	RchiOBHm_Chr2g0097141 RchiOBHm_Chr5g0051581
rosa_laevigata	RLG00000016706 RLG00000034873
rosa_multiflora	Rmu_co8502141.1_g000001 Rmu_sc0001182.1_g000006 Rmu_ssc0000089.1_g000039
rosa_roxburghii	Rroxscaffold_1G00027090 Rroxscaffold_2G00144940
rosa_rugosa	Rorug02G0064600 Rorug05G0279000
rosa_samantha	Rh2AG111700 Rh2BG113700 Rh2CG116000 Rh2DG115000 Rh5AG350100 Rh5BG361900 Rh5CG386800 Rh5DG376800
rosa_wichuraiana	Rw2G008690 Rw5G033130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	431
AccB1I	GGYRCC	3 cut(s)	1453, 1887, 4091
AccB7I	CCANNNNNTGG	3 cut(s)	617, 2953, 3735
AccBSI	CCGCTC	1 cut(s)	750
AccI	GTMKAC	4 cut(s)	2817, 2999, 3081, 3669
AccII	CGCG	1 cut(s)	658
AciI	CCGC	4 cut(s)	9, 12, 750, 3972
AclWI	GGATC	6 cut(s)	13, 1751, 2354, 2449, 3212, 4466
AcoI	YGGCCR	4 cut(s)	127, 235, 3794, 3998
AcuI	CTGAAG	4 cut(s)	957, 1053, 2203, 3621
AfaI	GTAC	9 cut(s)	309, 391, 463, 677, 1289, 1571, 1788, 2384, 4081
AflII	CTTAAG	1 cut(s)	3236
AflIII	ACRYGT	3 cut(s)	1264, 2264, 2385
AgeI	ACCGGT	3 cut(s)	1010, 3247, 4088
AhdI	GACNNNNNGTC	1 cut(s)	3858
AjiI	CACGTC	1 cut(s)	1128
AjnI	CCWGG	8 cut(s)	1565, 2071, 3003, 3658, 3696, 3810, 4303, 4515
AjuI	GAANNNNNNNTTGG	6 cut(s)	46, 78, 514, 546, 2498, 2530
AleI	CACNNNNGTG	1 cut(s)	1265
AloI	GAACNNNNNNTCC	4 cut(s)	3318, 3350, 4608, 4640
Alw21I	GWGCWC	1 cut(s)	4549
Alw26I	GTCTC	9 cut(s)	186, 1208, 1297, 1863, 2190, 2387, 2441, 4113, 4198
AlwI	GGATC	6 cut(s)	13, 1751, 2354, 2449, 3212, 4466
AlwNI	CAGNNNCTG	1 cut(s)	1766
Ama87I	CYCGRG	1 cut(s)	1300
ApeKI	GCWGC	9 cut(s)	203, 206, 877, 1066, 1591, 1807, 2223, 3968, 4067
ArsI	GACNNNNNNTTYG	4 cut(s)	777, 809, 1093, 1125
AseI	ATTAAT	3 cut(s)	1152, 3528, 3678
AsiGI	ACCGGT	3 cut(s)	1010, 3247, 4088
Asp700I	GAANNNNTTC	4 cut(s)	2414, 3333, 3719, 3984
AspLEI	GCGC	1 cut(s)	658
AspS9I	GGNCC	6 cut(s)	328, 1967, 2119, 3686, 4321, 4420
AsuC2I	CCSGG	1 cut(s)	4051
AsuHPI	GGTGA	9 cut(s)	818, 1262, 1525, 2255, 3274, 3296, 3649, 3910, 4351
AsuII	TTCGAA	4 cut(s)	1552, 3016, 4075, 4250
AvaI	CYCGRG	1 cut(s)	1300
AvaII	GGWCC	4 cut(s)	328, 1967, 2119, 4321
AxyI	CCTNAGG	1 cut(s)	4329
BaeGI	GKGCMC	2 cut(s)	1456, 4096
BaeI	ACNNNNGTAYC	2 cut(s)	4364, 4397
BalI	TGGCCA	2 cut(s)	237, 4000
BamHI	GGATCC	1 cut(s)	5
BanI	GGYRCC	3 cut(s)	1453, 1887, 4091
BanII	GRGCYC	1 cut(s)	560
BarI	GAAGNNNNNNTAC	2 cut(s)	1417, 1449
BauI	CACGAG	1 cut(s)	4195
BbsI	GAAGAC	5 cut(s)	60, 361, 1425, 1856, 4683
Bbv12I	GWGCWC	1 cut(s)	4549
BbvCI	CCTCAGC	1 cut(s)	4476
BbvI	GCAGC	9 cut(s)	190, 193, 864, 1053, 1603, 1819, 2235, 3980, 4054
BccI	CCATC	9 cut(s)	380, 403, 1385, 1594, 1853, 2396, 3513, 3880, 4472
BceAI	ACGGC	5 cut(s)	114, 1700, 2279, 3539, 4600
BcgI	CGANNNNNNTGC	4 cut(s)	1786, 1820, 4121, 4155
BciT130I	CCWGG	8 cut(s)	1567, 2073, 3005, 3660, 3698, 3812, 4305, 4517
BciVI	GTATCC	2 cut(s)	1436, 2558
BclI	TGATCA	2 cut(s)	859, 4240
BcnI	CCSGG	1 cut(s)	4051
BcoDI	GTCTC	9 cut(s)	186, 1208, 1297, 1863, 2190, 2387, 2441, 4113, 4198
BfaI	CTAG	3 cut(s)	1059, 3297, 3380
BfmI	CTRYAG	4 cut(s)	706, 2190, 3085, 4143
BfrI	CTTAAG	1 cut(s)	3236
BfuI	GTATCC	2 cut(s)	1436, 2558
BglI	GCCNNNNNGGC	1 cut(s)	2566
BglII	AGATCT	2 cut(s)	537, 2313
BmcAI	AGTACT	2 cut(s)	463, 4081
Bme1390I	CCNGG	9 cut(s)	1567, 2073, 3005, 3660, 3698, 3812, 4051, 4305, 4517
Bme18I	GGWCC	4 cut(s)	328, 1967, 2119, 4321
BmeRI	GACNNNNNGTC	1 cut(s)	3858
BmeT110I	CYCGRG	1 cut(s)	1300
BmgBI	CACGTC	1 cut(s)	1128
BmgT120I	GGNCC	6 cut(s)	328, 1967, 2119, 3686, 4321, 4420
BmiI	GGNNCC	8 cut(s)	7, 330, 1455, 1889, 1968, 2250, 4093, 4202
BmrFI	CCNGG	9 cut(s)	1567, 2073, 3005, 3660, 3698, 3812, 4051, 4305, 4517
BmrI	ACTGGG	2 cut(s)	172, 4450
BmuI	ACTGGG	2 cut(s)	172, 4450
BpiI	GAAGAC	5 cut(s)	60, 361, 1425, 1856, 4683
BpmI	CTGGAG	4 cut(s)	750, 2040, 3833, 3942
Bpu10I	CCTNAGC	1 cut(s)	4476
Bpu14I	TTCGAA	4 cut(s)	1552, 3016, 4075, 4250
BpuEI	CTTGAG	5 cut(s)	425, 1646, 3902, 4539, 4646
BpuMI	CCSGG	1 cut(s)	4051
Bsa29I	ATCGAT	1 cut(s)	525
BsaI	GGTCTC	1 cut(s)	2190
BsaWI	WCCGGW	3 cut(s)	1010, 3247, 4088
BsaXI	ACNNNNNCTCC	6 cut(s)	20, 50, 3318, 3348, 3564, 3594
Bse118I	RCCGGY	5 cut(s)	1010, 2670, 3247, 3478, 4088
Bse21I	CCTNAGG	1 cut(s)	4329
Bse3DI	GCAATG	4 cut(s)	1998, 3186, 3544, 4156
BseBI	CCWGG	8 cut(s)	1567, 2073, 3005, 3660, 3698, 3812, 4305, 4517
BseCI	ATCGAT	1 cut(s)	525
BseGI	GGATG	8 cut(s)	1016, 1225, 1479, 2527, 3410, 3505, 4275, 4507
BseMI	GCAATG	4 cut(s)	1998, 3186, 3544, 4156
BseRI	GAGGAG	3 cut(s)	2111, 2261, 3163
BseSI	GKGCMC	2 cut(s)	1456, 4096
BseXI	GCAGC	9 cut(s)	190, 193, 864, 1053, 1603, 1819, 2235, 3980, 4054
BseYI	CCCAGC	1 cut(s)	2909
BsgI	GTGCAG	1 cut(s)	4329
Bsh1236I	CGCG	1 cut(s)	658
BshNI	GGYRCC	3 cut(s)	1453, 1887, 4091
BshTI	ACCGGT	3 cut(s)	1010, 3247, 4088
BshVI	ATCGAT	1 cut(s)	525
BsiHKAI	GWGCWC	1 cut(s)	4549
BsiHKCI	CYCGRG	1 cut(s)	1300
BsiSI	CCGG	7 cut(s)	1011, 2671, 3248, 3479, 3793, 4050, 4089
BslFI	GGGAC	3 cut(s)	1980, 2048, 2905
BsmAI	GTCTC	9 cut(s)	186, 1208, 1297, 1863, 2190, 2387, 2441, 4113, 4198
BsmBI	CGTCTC	2 cut(s)	1208, 2387
BsmFI	GGGAC	3 cut(s)	1980, 2048, 2905
BsmI	GAATGC	3 cut(s)	1085, 2593, 3767
Bso31I	GGTCTC	1 cut(s)	2190
BsoBI	CYCGRG	1 cut(s)	1300
Bsp119I	TTCGAA	4 cut(s)	1552, 3016, 4075, 4250
Bsp1286I	GDGCHC	4 cut(s)	560, 1456, 4096, 4549
Bsp1407I	TGTACA	2 cut(s)	1287, 1786
Bsp19I	CCATGG	5 cut(s)	916, 1735, 2419, 2561, 4376
BspACI	CCGC	4 cut(s)	9, 12, 750, 3972
BspDI	ATCGAT	1 cut(s)	525
BspFNI	CGCG	1 cut(s)	658
BspHI	TCATGA	1 cut(s)	2842
BspLI	GGNNCC	8 cut(s)	7, 330, 1455, 1889, 1968, 2250, 4093, 4202
BspMAI	CTGCAG	1 cut(s)	2194
BspPI	GGATC	6 cut(s)	13, 1751, 2354, 2449, 3212, 4466
BspQI	GCTCTTC	2 cut(s)	162, 3468
BspT104I	TTCGAA	4 cut(s)	1552, 3016, 4075, 4250
BspT107I	GGYRCC	3 cut(s)	1453, 1887, 4091
BspTI	CTTAAG	1 cut(s)	3236
BspTNI	GGTCTC	1 cut(s)	2190
BsrBI	CCGCTC	1 cut(s)	750
BsrDI	GCAATG	4 cut(s)	1998, 3186, 3544, 4156
BsrFI	RCCGGY	5 cut(s)	1010, 2670, 3247, 3478, 4088
BsrGI	TGTACA	2 cut(s)	1287, 1786
BssAI	RCCGGY	5 cut(s)	1010, 2670, 3247, 3478, 4088
BssNAI	GTATAC	1 cut(s)	2818
BssSI	CACGAG	1 cut(s)	4195
BssT1I	CCWWGG	6 cut(s)	916, 1735, 2419, 2561, 3516, 4376
Bst1107I	GTATAC	1 cut(s)	2818
Bst2BI	CACGAG	1 cut(s)	4195
Bst2UI	CCWGG	8 cut(s)	1567, 2073, 3005, 3660, 3698, 3812, 4305, 4517
BstAFI	CTTAAG	1 cut(s)	3236
BstAPI	GCANNNNNTGC	2 cut(s)	1469, 4091
BstAUI	TGTACA	2 cut(s)	1287, 1786
BstBI	TTCGAA	4 cut(s)	1552, 3016, 4075, 4250
BstDSI	CCRYGG	6 cut(s)	916, 1735, 1952, 2419, 2561, 4376
BstENI	CCTNNNNNAGG	4 cut(s)	2685, 3225, 4143, 4328
BstF5I	GGATG	8 cut(s)	1016, 1225, 1479, 2527, 3410, 3505, 4275, 4507
BstFNI	CGCG	1 cut(s)	658
BstHHI	GCGC	1 cut(s)	658
BstMAI	GTCTC	9 cut(s)	186, 1208, 1297, 1863, 2190, 2387, 2441, 4113, 4198
BstNI	CCWGG	8 cut(s)	1567, 2073, 3005, 3660, 3698, 3812, 4305, 4517
BstNSI	RCATGY	8 cut(s)	176, 213, 1268, 2217, 2268, 2294, 2389, 4016
BstSCI	CCNGG	9 cut(s)	1565, 2071, 3003, 3658, 3696, 3810, 4049, 4303, 4515
BstSFI	CTRYAG	4 cut(s)	706, 2190, 3085, 4143
BstSLI	GKGCMC	2 cut(s)	1456, 4096
BstUI	CGCG	1 cut(s)	658
BstV1I	GCAGC	9 cut(s)	190, 193, 864, 1053, 1603, 1819, 2235, 3980, 4054
BstV2I	GAAGAC	5 cut(s)	60, 361, 1425, 1856, 4683
BstX2I	RGATCY	6 cut(s)	5, 537, 2313, 2359, 2441, 4471
BstXI	CCANNNNNNTGG	1 cut(s)	570
BstYI	RGATCY	6 cut(s)	5, 537, 2313, 2359, 2441, 4471
BstZ17I	GTATAC	1 cut(s)	2818
Bsu15I	ATCGAT	1 cut(s)	525
Bsu36I	CCTNAGG	1 cut(s)	4329
BsuI	GTATCC	2 cut(s)	1436, 2558
BsuTUI	ATCGAT	1 cut(s)	525
BtgI	CCRYGG	6 cut(s)	916, 1735, 1952, 2419, 2561, 4376
BtgZI	GCGATG	3 cut(s)	177, 247, 1572
BtrI	CACGTC	1 cut(s)	1128
BtsCI	GGATG	8 cut(s)	1016, 1225, 1479, 2527, 3410, 3505, 4275, 4507
BtsI	GCAGTG	5 cut(s)	778, 888, 2233, 2463, 4677
CaiI	CAGNNNCTG	1 cut(s)	1766
CciI	TCATGA	1 cut(s)	2842
CfoI	GCGC	1 cut(s)	658
Cfr10I	RCCGGY	5 cut(s)	1010, 2670, 3247, 3478, 4088
Cfr13I	GGNCC	6 cut(s)	328, 1967, 2119, 3686, 4321, 4420
ClaI	ATCGAT	1 cut(s)	525
CseI	GACGC	1 cut(s)	4351
Csp6I	GTAC	9 cut(s)	308, 390, 462, 676, 1288, 1570, 1787, 2383, 4080
CspAI	ACCGGT	3 cut(s)	1010, 3247, 4088
CviQI	GTAC	9 cut(s)	308, 390, 462, 676, 1288, 1570, 1787, 2383, 4080
DraI	TTTAAA	1 cut(s)	2788
DriI	GACNNNNNGTC	1 cut(s)	3858
EaeI	YGGCCR	4 cut(s)	127, 235, 3794, 3998
Eam1105I	GACNNNNNGTC	1 cut(s)	3858
Eco130I	CCWWGG	6 cut(s)	916, 1735, 2419, 2561, 3516, 4376
Eco147I	AGGCCT	1 cut(s)	3056
Eco24I	GRGCYC	1 cut(s)	560
Eco31I	GGTCTC	1 cut(s)	2190
Eco32I	GATATC	1 cut(s)	979
Eco47I	GGWCC	4 cut(s)	328, 1967, 2119, 4321
Eco57I	CTGAAG	4 cut(s)	957, 1053, 2203, 3621
Eco81I	CCTNAGG	1 cut(s)	4329
Eco88I	CYCGRG	1 cut(s)	1300
EcoNI	CCTNNNNNAGG	4 cut(s)	2685, 3225, 4143, 4328
EcoO109I	RGGNCCY	5 cut(s)	328, 1967, 3686, 4321, 4420
EcoRI	GAATTC	3 cut(s)	2155, 2794, 4524
EcoRII	CCWGG	8 cut(s)	1565, 2071, 3003, 3658, 3696, 3810, 4303, 4515
EcoRV	GATATC	1 cut(s)	979
EcoT14I	CCWWGG	6 cut(s)	916, 1735, 2419, 2561, 3516, 4376
EcoT22I	ATGCAT	1 cut(s)	4014
EcoT38I	GRGCYC	1 cut(s)	560
ErhI	CCWWGG	6 cut(s)	916, 1735, 2419, 2561, 3516, 4376
Esp3I	CGTCTC	2 cut(s)	1208, 2387
FalI	AAGNNNNNCTT	8 cut(s)	285, 317, 708, 740, 3219, 3251, 3972, 4004
FaqI	GGGAC	3 cut(s)	1980, 2048, 2905
FbaI	TGATCA	2 cut(s)	859, 4240
FblI	GTMKAC	4 cut(s)	2817, 2999, 3081, 3669
FokI	GGATG	8 cut(s)	1003, 1232, 1466, 2534, 3417, 3492, 4262, 4494
FriOI	GRGCYC	1 cut(s)	560
FspBI	CTAG	3 cut(s)	1059, 3297, 3380
GlaI	GCGC	1 cut(s)	657
GsaI	CCCAGC	1 cut(s)	2913
GsuI	CTGGAG	4 cut(s)	750, 2040, 3833, 3942
HapII	CCGG	7 cut(s)	1011, 2671, 3248, 3479, 3793, 4050, 4089
HgaI	GACGC	1 cut(s)	4351
HhaI	GCGC	1 cut(s)	658
Hin6I	GCGC	1 cut(s)	656
HinP1I	GCGC	1 cut(s)	656
HincII	GTYRAC	1 cut(s)	1237
HindII	GTYRAC	1 cut(s)	1237
HindIII	AAGCTT	3 cut(s)	102, 2012, 2931
HpaII	CCGG	7 cut(s)	1011, 2671, 3248, 3479, 3793, 4050, 4089
HphI	GGTGA	9 cut(s)	818, 1262, 1525, 2255, 3274, 3296, 3649, 3910, 4351
Hpy166II	GTNNAC	9 cut(s)	676, 1237, 2506, 2818, 3000, 3082, 3253, 3670, 4366
Hpy8I	GTNNAC	9 cut(s)	676, 1237, 2506, 2818, 3000, 3082, 3253, 3670, 4366
Hpy99I	CGWCG	2 cut(s)	1132, 1677
HpyCH4IV	ACGT	4 cut(s)	1108, 1127, 1672, 2396
HpySE526I	ACGT	4 cut(s)	1108, 1127, 1672, 2396
HspAI	GCGC	1 cut(s)	656
KroI	GCCGGC	1 cut(s)	3478
KroNI	GCCGGC	1 cut(s)	3480
Ksp22I	TGATCA	2 cut(s)	859, 4240
LguI	GCTCTTC	2 cut(s)	162, 3468
Lsp1109I	GCAGC	9 cut(s)	190, 193, 864, 1053, 1603, 1819, 2235, 3980, 4054
MaeI	CTAG	3 cut(s)	1059, 3297, 3380
MaeII	ACGT	4 cut(s)	1108, 1127, 1672, 2396
MaeIII	GTNAC	8 cut(s)	43, 545, 1354, 1742, 3243, 3448, 3621, 4339
MbiI	CCGCTC	1 cut(s)	750
MfeI	CAATTG	2 cut(s)	615, 4125
MflI	RGATCY	6 cut(s)	5, 537, 2313, 2359, 2441, 4471
MhlI	GDGCHC	4 cut(s)	560, 1456, 4096, 4549
MlsI	TGGCCA	2 cut(s)	237, 4000
MluNI	TGGCCA	2 cut(s)	237, 4000
MlyI	GAGTC	6 cut(s)	260, 355, 914, 1292, 3521, 4627
MmeI	TCCRAC	5 cut(s)	967, 1296, 3520, 4416, 4655
Mox20I	TGGCCA	2 cut(s)	237, 4000
Mph1103I	ATGCAT	1 cut(s)	4014
MroNI	GCCGGC	1 cut(s)	3478
MroXI	GAANNNNTTC	4 cut(s)	2414, 3333, 3719, 3984
MscI	TGGCCA	2 cut(s)	237, 4000
MslI	CAYNNNNRTG	5 cut(s)	671, 1265, 2847, 3213, 3260
Msp20I	TGGCCA	2 cut(s)	237, 4000
MspA1I	CMGCKG	2 cut(s)	877, 1810
MspCI	CTTAAG	1 cut(s)	3236
MspI	CCGG	7 cut(s)	1011, 2671, 3248, 3479, 3793, 4050, 4089
MspR9I	CCNGG	9 cut(s)	1567, 2073, 3005, 3660, 3698, 3812, 4051, 4305, 4517
MunI	CAATTG	2 cut(s)	615, 4125
Mva1269I	GAATGC	3 cut(s)	1085, 2593, 3767
MvaI	CCWGG	8 cut(s)	1567, 2073, 3005, 3660, 3698, 3812, 4305, 4517
MvnI	CGCG	1 cut(s)	658
NaeI	GCCGGC	1 cut(s)	3480
NciI	CCSGG	1 cut(s)	4051
NcoI	CCATGG	5 cut(s)	916, 1735, 2419, 2561, 4376
NgoMIV	GCCGGC	1 cut(s)	3478
NlaIV	GGNNCC	8 cut(s)	7, 330, 1455, 1889, 1968, 2250, 4093, 4202
NmuCI	GTSAC	3 cut(s)	43, 3621, 4339
NsiI	ATGCAT	1 cut(s)	4014
NspI	RCATGY	8 cut(s)	176, 213, 1268, 2217, 2268, 2294, 2389, 4016
NspV	TTCGAA	4 cut(s)	1552, 3016, 4075, 4250
OliI	CACNNNNGTG	1 cut(s)	1265
PaeI	GCATGC	3 cut(s)	176, 2217, 2294
PaeR7I	CTCGAG	1 cut(s)	1300
PagI	TCATGA	1 cut(s)	2842
PceI	AGGCCT	1 cut(s)	3056
PciI	ACATGT	3 cut(s)	1264, 2264, 2385
PciSI	GCTCTTC	2 cut(s)	162, 3468
PctI	GAATGC	3 cut(s)	1085, 2593, 3767
PdiI	GCCGGC	1 cut(s)	3480
PdmI	GAANNNNTTC	4 cut(s)	2414, 3333, 3719, 3984
PfeI	GAWTC	9 cut(s)	522, 1937, 2063, 2917, 3293, 3362, 3720, 4212, 4291
PflFI	GACNNNGTC	1 cut(s)	4190
PflMI	CCANNNNNTGG	3 cut(s)	617, 2953, 3735
PfoI	TCCNGGA	2 cut(s)	3810, 4049
PinAI	ACCGGT	3 cut(s)	1010, 3247, 4088
PleI	GAGTC	6 cut(s)	259, 355, 913, 1292, 3520, 4627
PpsI	GAGTC	6 cut(s)	259, 355, 913, 1292, 3520, 4627
PpuMI	RGGWCCY	3 cut(s)	328, 1967, 4321
PscI	ACATGT	3 cut(s)	1264, 2264, 2385
PshBI	ATTAAT	3 cut(s)	1152, 3528, 3678
PsiI	TTATAA	1 cut(s)	431
Psp5II	RGGWCCY	3 cut(s)	328, 1967, 4321
Psp6I	CCWGG	8 cut(s)	1565, 2071, 3003, 3658, 3696, 3810, 4303, 4515
PspFI	CCCAGC	1 cut(s)	2909
PspGI	CCWGG	8 cut(s)	1565, 2071, 3003, 3658, 3696, 3810, 4303, 4515
PspN4I	GGNNCC	8 cut(s)	7, 330, 1455, 1889, 1968, 2250, 4093, 4202
PspPI	GGNCC	6 cut(s)	328, 1967, 2119, 3686, 4321, 4420
PspPPI	RGGWCCY	3 cut(s)	328, 1967, 4321
PstI	CTGCAG	1 cut(s)	2194
PstNI	CAGNNNCTG	1 cut(s)	1766
PsuI	RGATCY	6 cut(s)	5, 537, 2313, 2359, 2441, 4471
PsyI	GACNNNGTC	1 cut(s)	4190
PvuII	CAGCTG	2 cut(s)	877, 1810
RsaI	GTAC	9 cut(s)	309, 391, 463, 677, 1289, 1571, 1788, 2384, 4081
RsaNI	GTAC	9 cut(s)	308, 390, 462, 676, 1288, 1570, 1787, 2383, 4080
RseI	CAYNNNNRTG	5 cut(s)	671, 1265, 2847, 3213, 3260
SapI	GCTCTTC	2 cut(s)	162, 3468
Sau96I	GGNCC	6 cut(s)	328, 1967, 2119, 3686, 4321, 4420
ScaI	AGTACT	2 cut(s)	463, 4081
SchI	GAGTC	6 cut(s)	260, 355, 914, 1292, 3521, 4627
ScrFI	CCNGG	9 cut(s)	1567, 2073, 3005, 3660, 3698, 3812, 4051, 4305, 4517
SduI	GDGCHC	4 cut(s)	560, 1456, 4096, 4549
SfcI	CTRYAG	4 cut(s)	706, 2190, 3085, 4143
Sfr274I	CTCGAG	1 cut(s)	1300
SfuI	TTCGAA	4 cut(s)	1552, 3016, 4075, 4250
SinI	GGWCC	4 cut(s)	328, 1967, 2119, 4321
SlaI	CTCGAG	1 cut(s)	1300
SmiMI	CAYNNNNRTG	5 cut(s)	671, 1265, 2847, 3213, 3260
SmlI	CTYRAG	7 cut(s)	404, 1300, 1661, 3236, 3881, 4554, 4661
SmoI	CTYRAG	7 cut(s)	404, 1300, 1661, 3236, 3881, 4554, 4661
SphI	GCATGC	3 cut(s)	176, 2217, 2294
SseBI	AGGCCT	1 cut(s)	3056
SsiI	CCGC	4 cut(s)	9, 12, 750, 3972
SspI	AATATT	3 cut(s)	1193, 1803, 2657
SspMI	CTAG	3 cut(s)	1059, 3297, 3380
StuI	AGGCCT	1 cut(s)	3056
StyD4I	CCNGG	9 cut(s)	1565, 2071, 3003, 3658, 3696, 3810, 4049, 4303, 4515
StyI	CCWWGG	6 cut(s)	916, 1735, 2419, 2561, 3516, 4376
TaiI	ACGT	4 cut(s)	1111, 1130, 1675, 2399
TaqII	GACCGA	1 cut(s)	2711
TatI	WGTACW	5 cut(s)	461, 1287, 1786, 2382, 4079
TauI	GCSGC	2 cut(s)	14, 3974
TfiI	GAWTC	9 cut(s)	522, 1937, 2063, 2917, 3293, 3362, 3720, 4212, 4291
TseFI	GTSAC	3 cut(s)	43, 3621, 4339
TseI	GCWGC	9 cut(s)	203, 206, 877, 1066, 1591, 1807, 2223, 3968, 4067
Tsp45I	GTSAC	3 cut(s)	43, 3621, 4339
TspGWI	ACGGA	3 cut(s)	1232, 3848, 4091
Tth111I	GACNNNGTC	1 cut(s)	4190
Van91I	CCANNNNNTGG	3 cut(s)	617, 2953, 3735
Vha464I	CTTAAG	1 cut(s)	3236
VpaK11BI	GGWCC	4 cut(s)	328, 1967, 2119, 4321
VspI	ATTAAT	3 cut(s)	1152, 3528, 3678
XagI	CCTNNNNNAGG	4 cut(s)	2685, 3225, 4143, 4328
XbaI	TCTAGA	1 cut(s)	3296
XceI	RCATGY	8 cut(s)	176, 213, 1268, 2217, 2268, 2294, 2389, 4016
XcmI	CCANNNNNNNNNTGG	3 cut(s)	3704, 4168, 4311
XhoI	CTCGAG	1 cut(s)	1300
XmiI	GTMKAC	4 cut(s)	2817, 2999, 3081, 3669
XmnI	GAANNNNTTC	4 cut(s)	2414, 3333, 3719, 3984
XspI	CTAG	3 cut(s)	1059, 3297, 3380
ZrmI	AGTACT	2 cut(s)	463, 4081
Zsp2I	ATGCAT	1 cut(s)	4014

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.