Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5B

Orthogroup ID

OG0002932

Physical Location & Seq

Genomic Coordinates Reverse (-)

56634261 .. 56642220

Gene Structure 7960 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5BG361900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4701 bp

ATGGGATCCGCCGCAGGATTAGAACCCGACCCTCCCACCACAGTGACTAAACCAACATCTTCTTCGTCTTCCTCTGGAATGACAAAGAAGAAGGGCAAAAAAGCTTCTCAAAAGGAAGCACCTACTGGCCGTGGAGGTAGGAAGCGAAATTTGCCTCAGAGCGATGAAGACCATGCCCAGTCTCGCAAAATGCCCACGCGTGCTGCTGCATGTAAAGACTTTAAGGAGAAGTCTGTCCATATTACTGATGAGTCTGATCTCATCGCAAGCAAGGAGGAACAGATTGTGCCAGAAGAAGGTCTTGCTGTACGCTTGACTTGTGGCAAGGACCCAGATAATGAGAAGCCAAGCAGAAGACTGACTCAATTTATCTTGCATAGTGAAGATGGTACTGCAAGACCACTTGAGATGGTGGAAGCTGGTCTGTTTATAACTGGCACTATATTGCCTCTTCGTGAAAGTACTGAGAAGGACAAGGACAAGGACAGCAGTATTAAATGTGAAGGTTTTGGGCGAATCGATGCTTGGAAGATCTCTGGTTACGATGAGGGCTCTCCAATGATATGGCTTTCAACTAAAGTTGCCGATTATTGTTGTGTTAAACCAGCAATTGGCTACAAGAAGCACTATGATCTCTTCTTTGAGAAAGCGCGTGCTTGCATAGAGGTGTACCAGAAACTTTCAAAATGTAAATCTGGCTGTAGTCTTGAAGAATTACTTGCTGGAGTTGCTCGATCATTGAGCGGAAGCAAATTCTTTTCTGGCAGTGCATCTATCAAAGAATTTGTCCTATCTCATGGTGAGTTTATCTATAAACGATTGATAGGTTTGGAGCAGTCATTGAAGAAGAATGATCAGATATTGGCACAGTTGCCTGTTCTCACTGCCCTCAGAGATGAGTCTATAAACCATGGATATTATGAGCAATCAGAACCAGTAGCTTCAAGTGCTACCTTAAAGATTGCTTCAGATATCAGAGTTGGAGGGAATGAACTGAGAACAACCGGTTCATCCATGGTTGAAGCTGAAGAAGATGAAGATGCAAAGTTAGCTAGACTGCTGCAAGAAGAAGAATGCTTTAAGTCTATGAAACAAAGGAAACGTCAGGGTGCTACCTCCACGTCGAGTAAATTCTACATCAAAATTAATGAAGATGAAATTGCTAATGATTATCCTGAACCTAAATATTATGACAATTCCATTGAAGAGACGGATGAGTTTATTGTTGTTGACAGTGAGGCTGGTGATCTTCCCACACATGTGCTTGATAATTGGTGTCTGTACAATTTAGACTCGAGACTGATTTCGTTGGAGCTTCTTCCAATGACAGATTGCCCAGATATTGATGTTACAATTTTTGGGTCAGGGGTTATGTGTGAAGATGATGGAAGCTGTGGCTTTAATCTCGATGCTGACAGTATCCAGTCTTCTTCAAGTGGTTCAGAGGCACCAGTAGCAGAAGATGCTTATGGCATCCCAATTTATTTGAGTGCCATAAAGGAATGGGTGATAGAATGGGGAGCATCAATGATCTCAGTATCAATTCGAACAGAGCTGGCCTGGTACAGACTTGGCAAGCCATCACAGCAGTATGCTTTGTGGTATGAACCAATACTGAAAACAGCAAGGGTTGGGAGAAGTATAATCACTATGCTCAAGGCTGAACGTCGAGTAGCACGGCTTTCTTTTGCAGATGTCATTAAACGATTGTCAGAGTTCCCAAAGGACCATGGTGGTTACATTTCTTCTGATCCAGCATCTGTTGAGAGATATGTGGTTGTACATGGAGCGATAATATTGCAGCTGTTTTCACAGTTTCCAGATGTGAAGATTAAAAAATGTTCATTTCTTGATGGTCTTCGAGACAAAATGGAGAAGAGGCACCATACTAAATGGGAAGTAAAGAGCAAGAAAATTTTGCAGAAGAGTGGATCAAATTTGAACCCAAGGGCATCAATGGGACCTGTGGTTTCCAAGAGGAAGGCAATGCAGGCTACAACAACAAAGCTTATCAACAGAATTTGGGGGGACTACTATTCAAATTACTCTCCAGTAGATTCAAGCCCAGGAGCTACTTGTGGAAAGGTAGAGGAGAATGAAGTTATAGAAGAGGTCCAAGAGGATATAGAAGAGTATGATGTAGATGAGAATTCATCAGTAATGGAACAAGCCCAAAGGTCTTCTGCAGTTTCAAGAAAGCCTAAAGCATGCTTCAGCAGCAGTGAAATTTTGTGGGGTGAGGAGCCTGTGGGAAAAACCTGTTCTGGTGAAGCTCTCTACAAGCATGCCGTTCTTTGTGGAGATGAGATCTCTGTGGGTGGTGCTGTTTTTGTGGAAATTGATGTGTCTAAAGATCCTGTTATCTATCTTGTTGAGTACATGTTTGAGACGTCAGATGGAAAGAAGATGTTCCATGGGAGAATGATGCAGAGAGGATCTGAGACTGTTCTGGGAAACACTGCTAATGAGAGAGAAGTGTTTTTAACTGATGAGTGCGTGAACTTGGCATTGGAGGATGTGGAACAGACTGTTGTTGTGGGTATCCAATCAATGCCATGGGGGCATCAGTATAGGAAGAAGAATGCTGATACTAATAGAATTGATAGAGCAAGAGCAGAAGAGAGGAAGAATAAAGGATTGCCAACAGAATATTACTGTAAAAGCCGGTATTGCCCTGAGAAAGGTGCGTTCTTTAGCCTTTCTCATCATACTATCGGTCTGGGTTCTGGTTTTTGCCACTCTTGCAAAGTTAATGAAGATGAAAAGGCCAAGGAAGTCTTTAAAGTGAATTCATCAAAGACTGGTTTTGTATACAGTGGAGCTGAGTATTTCATTCATGATTATGTTTATGTAAGCCCCTATCATTTTTCTGTGGAAAGGATTGGGACTGAGCTTTTCAAGGCTGGGAGGAATCTGGGATTGAAAGCTTATGTTGTGTGCCAAGTGCTGGAGATAATTACTGCAAAGGAATATAAACAACCTGATATGAAGTCTACCCAGGTTAAAGTTCGAAGGTTTTTCAGACCAGAGGACATTTCAGTAGAGAAGGCATACTGCTCTGATGTCAGAGAGGTCTACTACAGTGAAGAAACACACATTATCCCTGCTGATAGCATAGAAGGAAAATGTGAAGTTAGAAAGAGGAGTGATCTTCCAGAATGTGATGCTCCTGCAATGTTTGAGCATATTTTCTTCTGTGAGCATTTATATGATCCTTCTAAAGGGTCTCTTAAGCAGTTACCAGTTCACATCAAGGTGAGGCACTCAGCAGTTGGTGGTGATGCTGAATCTAGAAAGAAAAAGGGCAAATGCAAAGAAGGAGAAGATGTTCCAGAAGTTGAGAAACAGAGAGATGATTCTGAACACAAACGCCTAGCCACTTTGGATATATTTGCTGGATGTGGTGGCCTGTCAGAAGGTTTGCATCAGGCTGGTGTTTCAGTAACGAAGTGGGCAATCGAGTATGAAGAGCCGGCTGGACAGGCTTTCCAACTCAACCATTCTGAGTCCAAGGTGTTCATTAATAATTGCAATGTCATCTTGAAGGCCGTCATGGACAAATGTGGAGATATGGATGATTGCATCTCCACTACTGAAGCCAATGATTTAGCCTCATCACTTGATGAGAAGGTGAAAGACAATTTGCCCTTGCCAGGGCAAGTAGACTTTATTAATGGAGGCCCTCCATGCCAGGGATTTTCTGGTATGAATAGATTCAATACCAGCACTTGGAGTAAAGTCCAGTGTGAAATGATATTGGCATTCTTATCCTTTGCTGATTACTTCCGGCCAAAATACTTCCTCCTGGAGAATGTGAGGAACTTTGTGGCTTTTAATAAAGGACAGACATTCCGTCTGACTGTGGCTTCACTTCTTGAGATGGGTTATCAGGTGAGGTTTGGTGTTCTGGAAGCTGGAGCTTATGGGGTTTCTCAATCCCGAAAGCGTGCATTTATCTGGGCAGCCGCACCAGAAGAGGTTCTTCCAGATTGGCCAGAGCCAATGCATGTTTTTGGTGTGCCAGAGTTGAAGATCAATTTATCCGGGAACTCCTATTATGCTGCTGTTCGAAGTACTGCAACCGGTGCCCCATTCCGTGCCATAACAGTAAGAGACACAATTGGCGATCTTCCAGCTGTAGGCAATGGAGCTTCCAAGATCAATTTGGAATATGAAAGCGACCCTGTCTCGTGGTTTCAGAAGAGAATCAGAGGGAACATGGCTGTTTTGACTGATCACATTTCAAAAGAAATGAACGAGCTGAACCTCATCCGATGTACGAAGATTCCCAAGAGGCCAGGTGCAGATTGGCAGGACCTTCCTGAGGAAAAGGTGACGCTCTCTACTGGTCAAATAGTGGACTTGATACCATGGTGCTTGCCCAACACAGCTAAACGCCACAACCAGTGGAAGGGCCTGTTTGGAAGGTTAGACTGGGAAGGCAACTTCCCAACCTCCATCACAGATCCTCAGCCAATGGGTAAGGTGGGAATGTGCTTTCATCCTGACCAGGACAGAATTCTGACAGTTAGGGAATGTGCACGATCTCAAGGCTTTCCTGACAGCTACCAGTTTTATGGCAACATTCTTCACAAGCATAGGCAGATTGGAAATGCTGTTCCTCCGACTCTGGCTTATGCTCTGGGCAGAAAACTCAAGGAAGCAGTGGATAGCAAGAAGAACTCTTCTTCACAAGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000278 GO:0000281 GO:0000910 GO:0003006 GO:0003674 GO:0003824 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006349 GO:0006725 GO:0006807 GO:0007049 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008356 GO:0009292 GO:0009294 GO:0009790 GO:0009791 GO:0009793 GO:0009909 GO:0009910 GO:0009987 GO:0010069 GO:0010070 GO:0010154 GO:0010216 GO:0010424 GO:0010468 GO:0016740 GO:0016741 GO:0019222 GO:0022402 GO:0022414 GO:0032259 GO:0032501 GO:0032502 GO:0032776 GO:0034641 GO:0040029 GO:0043170 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044728 GO:0044764 GO:0046483 GO:0048316 GO:0048519 GO:0048580 GO:0048581 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0051093 GO:0051239 GO:0051241 GO:0051301 GO:0051704 GO:0060255 GO:0061458 GO:0061640 GO:0065007 GO:0071514 GO:0071704 GO:0090304 GO:1901360 GO:1903047 GO:2000026 GO:2000241 GO:2000242

KEGG Pathways

Metabolic & Signaling

ko00270 ko01100 ko05206 map00270 map01100 map05206

Pfam Domains

Protein Families

BAH DNA_methylase DNMT1-RFD

Protein Analysis

1566

Amino Acids

174.96

Weight (kDa)

5.92

Isoelectric Point (pI)

46.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DNMT1-RFD	PF12047	113 - 245	3.7e-31	Cytosine specific DNA methyltransferase replication foci domain
DNMT1-RFD	PF12047	416 - 572	3.8e-31	Cytosine specific DNA methyltransferase replication foci domain
BAH	PF01426	769 - 900	3.3e-19	BAH domain
BAH	PF01426	943 - 1081	4.4e-26	BAH domain
DNA_methylase	PF00145	1126 - 1553	2e-47	C-5 cytosine-specific DNA methylase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002932)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G08990 AT4G14140 AT4G14140 AT4G14140 AT5G49160 AT5G49160
fragaria_vesca	FvH4_1g10300 FvH4_1g10300
malus_domestica	MD02G1115100.v1.1 MD15G1231100.v1.1
prunus_persica	Prupe.7G060100_v2.0.a1 Prupe.7G183100_v2.0.a1 Prupe.7G183100_v2.0.a1
pyrus_communis	pycom02g09000 pycom02g09010 pycom15g20590 pycom15g20600
rosa_chinensis	RchiOBHm_Chr2g0097141 RchiOBHm_Chr5g0051581
rosa_laevigata	RLG00000016706 RLG00000034873
rosa_multiflora	Rmu_co8502141.1_g000001 Rmu_sc0001182.1_g000006 Rmu_ssc0000089.1_g000039
rosa_roxburghii	Rroxscaffold_1G00027090 Rroxscaffold_2G00144940
rosa_rugosa	Rorug02G0064600 Rorug05G0279000
rosa_samantha	Rh2AG111700 Rh2BG113700 Rh2CG116000 Rh2DG115000 Rh5AG350100 Rh5BG361900 Rh5CG386800 Rh5DG376800
rosa_wichuraiana	Rw2G008690 Rw5G033130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	431
AatII	GACGTC	1 cut(s)	2393
AccB1I	GGYRCC	3 cut(s)	1447, 1881, 4085
AccB7I	CCANNNNNTGG	3 cut(s)	611, 2947, 3729
AccBSI	CCGCTC	1 cut(s)	744
AccI	GTMKAC	4 cut(s)	2811, 2993, 3075, 3663
AccII	CGCG	2 cut(s)	199, 652
AciI	CCGC	4 cut(s)	9, 12, 744, 3966
AclWI	GGATC	7 cut(s)	13, 1745, 1939, 2348, 2443, 3206, 4460
AcoI	YGGCCR	3 cut(s)	127, 3788, 3992
AcuI	CTGAAG	4 cut(s)	951, 1047, 2197, 3615
AcyI	GRCGYC	1 cut(s)	2390
AflII	CTTAAG	1 cut(s)	3230
AflIII	ACRYGT	3 cut(s)	197, 1258, 2379
AgeI	ACCGGT	2 cut(s)	1004, 4082
AhdI	GACNNNNNGTC	1 cut(s)	3852
AjiI	CACGTC	1 cut(s)	1122
AjnI	CCWGG	8 cut(s)	1559, 2065, 2997, 3652, 3690, 3804, 4297, 4509
AjuI	GAANNNNNNNTTGG	6 cut(s)	46, 78, 508, 540, 2492, 2524
AleI	CACNNNNGTG	2 cut(s)	41, 1259
AloI	GAACNNNNNNTCC	4 cut(s)	3312, 3344, 4602, 4634
Alw21I	GWGCWC	1 cut(s)	4543
Alw26I	GTCTC	9 cut(s)	186, 1202, 1291, 1857, 2381, 2435, 3231, 4107, 4192
Alw44I	GTGCAC	1 cut(s)	4539
AlwI	GGATC	7 cut(s)	13, 1745, 1939, 2348, 2443, 3206, 4460
AlwNI	CAGNNNCTG	1 cut(s)	1760
Ama87I	CYCGRG	1 cut(s)	1294
ApaLI	GTGCAC	1 cut(s)	4539
ApeKI	GCWGC	7 cut(s)	203, 206, 1060, 1801, 2217, 3962, 4061
ArsI	GACNNNNNNTTYG	4 cut(s)	771, 803, 1087, 1119
AseI	ATTAAT	3 cut(s)	1146, 3522, 3672
AsiGI	ACCGGT	2 cut(s)	1004, 4082
Asp700I	GAANNNNTTC	4 cut(s)	2408, 3327, 3713, 3978
AspLEI	GCGC	1 cut(s)	652
AspS9I	GGNCC	7 cut(s)	328, 1726, 1961, 2113, 3680, 4315, 4414
AsuC2I	CCSGG	1 cut(s)	4045
AsuII	TTCGAA	3 cut(s)	1546, 3010, 4069
AvaI	CYCGRG	1 cut(s)	1294
AvaII	GGWCC	5 cut(s)	328, 1726, 1961, 2113, 4315
AxyI	CCTNAGG	1 cut(s)	4323
BaeGI	GKGCMC	2 cut(s)	4090, 4543
BaeI	ACNNNNGTAYC	2 cut(s)	4358, 4391
BalI	TGGCCA	1 cut(s)	3994
BamHI	GGATCC	1 cut(s)	5
BanI	GGYRCC	3 cut(s)	1447, 1881, 4085
BanII	GRGCYC	1 cut(s)	554
BarI	GAAGNNNNNNTAC	2 cut(s)	1411, 1443
BauI	CACGAG	1 cut(s)	4189
BbsI	GAAGAC	6 cut(s)	60, 174, 361, 1419, 1850, 2172
Bbv12I	GWGCWC	1 cut(s)	4543
BbvCI	CCTCAGC	1 cut(s)	4470
BbvI	GCAGC	7 cut(s)	190, 193, 1047, 1813, 2229, 3974, 4048
BccI	CCATC	8 cut(s)	380, 403, 1379, 1588, 1847, 2390, 3874, 4466
BceAI	ACGGC	4 cut(s)	114, 1694, 2273, 3533
BcgI	CGANNNNNNTGC	2 cut(s)	1780, 1814
BciT130I	CCWGG	8 cut(s)	1561, 2067, 2999, 3654, 3692, 3806, 4299, 4511
BciVI	GTATCC	2 cut(s)	1430, 2552
BclI	TGATCA	2 cut(s)	853, 4234
BcnI	CCSGG	1 cut(s)	4045
BcoDI	GTCTC	9 cut(s)	186, 1202, 1291, 1857, 2381, 2435, 3231, 4107, 4192
BfaI	CTAG	3 cut(s)	1053, 3291, 3374
BfmI	CTRYAG	4 cut(s)	700, 2184, 3079, 4137
BfrI	CTTAAG	1 cut(s)	3230
BfuI	GTATCC	2 cut(s)	1430, 2552
BglI	GCCNNNNNGGC	1 cut(s)	2560
BglII	AGATCT	2 cut(s)	531, 2307
BisI	GCNGC	9 cut(s)	12, 204, 207, 1061, 1802, 2218, 3963, 3966, 4062
BlsI	GCNGC	9 cut(s)	13, 205, 208, 1062, 1803, 2219, 3964, 3967, 4063
BmcAI	AGTACT	2 cut(s)	463, 4075
Bme1390I	CCNGG	9 cut(s)	1561, 2067, 2999, 3654, 3692, 3806, 4045, 4299, 4511
Bme18I	GGWCC	5 cut(s)	328, 1726, 1961, 2113, 4315
BmeRI	GACNNNNNGTC	1 cut(s)	3852
BmeT110I	CYCGRG	1 cut(s)	1294
BmgBI	CACGTC	1 cut(s)	1122
BmgT120I	GGNCC	7 cut(s)	328, 1726, 1961, 2113, 3680, 4315, 4414
BmiI	GGNNCC	7 cut(s)	7, 330, 1449, 1883, 1962, 2244, 4087
BmrFI	CCNGG	9 cut(s)	1561, 2067, 2999, 3654, 3692, 3806, 4045, 4299, 4511
BmrI	ACTGGG	2 cut(s)	172, 4444
BmuI	ACTGGG	2 cut(s)	172, 4444
BpiI	GAAGAC	6 cut(s)	60, 174, 361, 1419, 1850, 2172
BpmI	CTGGAG	5 cut(s)	744, 2034, 2969, 3827, 3936
Bpu10I	CCTNAGC	1 cut(s)	4470
Bpu14I	TTCGAA	3 cut(s)	1546, 3010, 4069
BpuEI	CTTGAG	5 cut(s)	425, 1640, 3896, 4533, 4640
BpuMI	CCSGG	1 cut(s)	4045
Bsa29I	ATCGAT	1 cut(s)	519
BsaHI	GRCGYC	1 cut(s)	2390
BsaI	GGTCTC	1 cut(s)	3231
BsaWI	WCCGGW	2 cut(s)	1004, 4082
BsaXI	ACNNNNNCTCC	2 cut(s)	3312, 3342
Bse118I	RCCGGY	4 cut(s)	1004, 2664, 3472, 4082
Bse21I	CCTNAGG	1 cut(s)	4323
Bse3DI	GCAATG	4 cut(s)	1992, 3180, 3538, 4150
BseBI	CCWGG	8 cut(s)	1561, 2067, 2999, 3654, 3692, 3806, 4299, 4511
BseCI	ATCGAT	1 cut(s)	519
BseGI	GGATG	8 cut(s)	1010, 1219, 1473, 2521, 3404, 3580, 4269, 4501
BseMI	GCAATG	4 cut(s)	1992, 3180, 3538, 4150
BseRI	GAGGAG	3 cut(s)	2105, 2255, 3157
BseSI	GKGCMC	2 cut(s)	4090, 4543
BseXI	GCAGC	7 cut(s)	190, 193, 1047, 1813, 2229, 3974, 4048
BseYI	CCCAGC	1 cut(s)	2903
BsgI	GTGCAG	1 cut(s)	4323
Bsh1236I	CGCG	2 cut(s)	199, 652
BshNI	GGYRCC	3 cut(s)	1447, 1881, 4085
BshTI	ACCGGT	2 cut(s)	1004, 4082
BshVI	ATCGAT	1 cut(s)	519
BsiHKAI	GWGCWC	1 cut(s)	4543
BsiHKCI	CYCGRG	1 cut(s)	1294
BsiSI	CCGG	6 cut(s)	1005, 2665, 3473, 3787, 4044, 4083
BslFI	GGGAC	3 cut(s)	1974, 2042, 2899
BsmAI	GTCTC	9 cut(s)	186, 1202, 1291, 1857, 2381, 2435, 3231, 4107, 4192
BsmBI	CGTCTC	2 cut(s)	1202, 2381
BsmFI	GGGAC	3 cut(s)	1974, 2042, 2899
BsmI	GAATGC	3 cut(s)	1079, 2587, 3761
Bso31I	GGTCTC	1 cut(s)	3231
BsoBI	CYCGRG	1 cut(s)	1294
Bsp119I	TTCGAA	3 cut(s)	1546, 3010, 4069
Bsp1286I	GDGCHC	3 cut(s)	554, 4090, 4543
Bsp1407I	TGTACA	2 cut(s)	1281, 1780
Bsp19I	CCATGG	6 cut(s)	910, 1014, 1729, 2413, 2555, 4370
BspACI	CCGC	4 cut(s)	9, 12, 744, 3966
BspDI	ATCGAT	1 cut(s)	519
BspFNI	CGCG	2 cut(s)	199, 652
BspHI	TCATGA	1 cut(s)	2836
BspLI	GGNNCC	7 cut(s)	7, 330, 1449, 1883, 1962, 2244, 4087
BspMAI	CTGCAG	1 cut(s)	2188
BspPI	GGATC	7 cut(s)	13, 1745, 1939, 2348, 2443, 3206, 4460
BspQI	GCTCTTC	1 cut(s)	3462
BspT104I	TTCGAA	3 cut(s)	1546, 3010, 4069
BspT107I	GGYRCC	3 cut(s)	1447, 1881, 4085
BspTI	CTTAAG	1 cut(s)	3230
BspTNI	GGTCTC	1 cut(s)	3231
BsrBI	CCGCTC	1 cut(s)	744
BsrDI	GCAATG	4 cut(s)	1992, 3180, 3538, 4150
BsrFI	RCCGGY	4 cut(s)	1004, 2664, 3472, 4082
BsrGI	TGTACA	2 cut(s)	1281, 1780
BssAI	RCCGGY	4 cut(s)	1004, 2664, 3472, 4082
BssNAI	GTATAC	1 cut(s)	2812
BssNI	GRCGYC	1 cut(s)	2390
BssSI	CACGAG	1 cut(s)	4189
BssT1I	CCWWGG	9 cut(s)	910, 1014, 1729, 1946, 2413, 2555, 2769, 3510, 4370
Bst1107I	GTATAC	1 cut(s)	2812
Bst2BI	CACGAG	1 cut(s)	4189
Bst2UI	CCWGG	8 cut(s)	1561, 2067, 2999, 3654, 3692, 3806, 4299, 4511
BstACI	GRCGYC	1 cut(s)	2390
BstAFI	CTTAAG	1 cut(s)	3230
BstAPI	GCANNNNNTGC	2 cut(s)	1463, 4085
BstAUI	TGTACA	2 cut(s)	1281, 1780
BstBI	TTCGAA	3 cut(s)	1546, 3010, 4069
BstDSI	CCRYGG	7 cut(s)	130, 910, 1014, 1729, 2413, 2555, 4370
BstENI	CCTNNNNNAGG	3 cut(s)	2679, 3219, 4322
BstF5I	GGATG	8 cut(s)	1010, 1219, 1473, 2521, 3404, 3580, 4269, 4501
BstFNI	CGCG	2 cut(s)	199, 652
BstHHI	GCGC	1 cut(s)	652
BstMAI	GTCTC	9 cut(s)	186, 1202, 1291, 1857, 2381, 2435, 3231, 4107, 4192
BstNI	CCWGG	8 cut(s)	1561, 2067, 2999, 3654, 3692, 3806, 4299, 4511
BstNSI	RCATGY	6 cut(s)	213, 1262, 2211, 2288, 2383, 4010
BstSCI	CCNGG	9 cut(s)	1559, 2065, 2997, 3652, 3690, 3804, 4043, 4297, 4509
BstSFI	CTRYAG	4 cut(s)	700, 2184, 3079, 4137
BstSLI	GKGCMC	2 cut(s)	4090, 4543
BstUI	CGCG	2 cut(s)	199, 652
BstV1I	GCAGC	7 cut(s)	190, 193, 1047, 1813, 2229, 3974, 4048
BstV2I	GAAGAC	6 cut(s)	60, 174, 361, 1419, 1850, 2172
BstX2I	RGATCY	6 cut(s)	5, 531, 2307, 2353, 2435, 4465
BstXI	CCANNNNNNTGG	2 cut(s)	564, 4577
BstYI	RGATCY	6 cut(s)	5, 531, 2307, 2353, 2435, 4465
BstZ17I	GTATAC	1 cut(s)	2812
Bsu15I	ATCGAT	1 cut(s)	519
Bsu36I	CCTNAGG	1 cut(s)	4323
BsuI	GTATCC	2 cut(s)	1430, 2552
BsuTUI	ATCGAT	1 cut(s)	519
BtgI	CCRYGG	7 cut(s)	130, 910, 1014, 1729, 2413, 2555, 4370
BtgZI	GCGATG	2 cut(s)	177, 247
BtrI	CACGTC	1 cut(s)	1122
BtsCI	GGATG	8 cut(s)	1010, 1219, 1473, 2521, 3404, 3580, 4269, 4501
BtsI	GCAGTG	5 cut(s)	772, 882, 2227, 2457, 4671
CaiI	CAGNNNCTG	1 cut(s)	1760
CciI	TCATGA	1 cut(s)	2836
CfoI	GCGC	1 cut(s)	652
Cfr10I	RCCGGY	4 cut(s)	1004, 2664, 3472, 4082
Cfr13I	GGNCC	7 cut(s)	328, 1726, 1961, 2113, 3680, 4315, 4414
ClaI	ATCGAT	1 cut(s)	519
CseI	GACGC	1 cut(s)	4345
CspAI	ACCGGT	2 cut(s)	1004, 4082
DraI	TTTAAA	1 cut(s)	2782
DriI	GACNNNNNGTC	1 cut(s)	3852
EaeI	YGGCCR	3 cut(s)	127, 3788, 3992
Eam1105I	GACNNNNNGTC	1 cut(s)	3852
Eco130I	CCWWGG	9 cut(s)	910, 1014, 1729, 1946, 2413, 2555, 2769, 3510, 4370
Eco24I	GRGCYC	1 cut(s)	554
Eco31I	GGTCTC	1 cut(s)	3231
Eco32I	GATATC	1 cut(s)	973
Eco47I	GGWCC	5 cut(s)	328, 1726, 1961, 2113, 4315
Eco57I	CTGAAG	4 cut(s)	951, 1047, 2197, 3615
Eco81I	CCTNAGG	1 cut(s)	4323
Eco88I	CYCGRG	1 cut(s)	1294
EcoNI	CCTNNNNNAGG	3 cut(s)	2679, 3219, 4322
EcoO109I	RGGNCCY	5 cut(s)	328, 1961, 3680, 4315, 4414
EcoRI	GAATTC	3 cut(s)	2149, 2788, 4518
EcoRII	CCWGG	8 cut(s)	1559, 2065, 2997, 3652, 3690, 3804, 4297, 4509
EcoRV	GATATC	1 cut(s)	973
EcoT14I	CCWWGG	9 cut(s)	910, 1014, 1729, 1946, 2413, 2555, 2769, 3510, 4370
EcoT22I	ATGCAT	1 cut(s)	4008
EcoT38I	GRGCYC	1 cut(s)	554
ErhI	CCWWGG	9 cut(s)	910, 1014, 1729, 1946, 2413, 2555, 2769, 3510, 4370
Esp3I	CGTCTC	2 cut(s)	1202, 2381
FaqI	GGGAC	3 cut(s)	1974, 2042, 2899
FbaI	TGATCA	2 cut(s)	853, 4234
FblI	GTMKAC	4 cut(s)	2811, 2993, 3075, 3663
Fnu4HI	GCNGC	9 cut(s)	12, 204, 207, 1061, 1802, 2218, 3963, 3966, 4062
FokI	GGATG	8 cut(s)	997, 1226, 1460, 2528, 3411, 3587, 4256, 4488
FriOI	GRGCYC	1 cut(s)	554
Fsp4HI	GCNGC	9 cut(s)	12, 204, 207, 1061, 1802, 2218, 3963, 3966, 4062
FspBI	CTAG	3 cut(s)	1053, 3291, 3374
GlaI	GCGC	1 cut(s)	651
GluI	GCNGC	9 cut(s)	12, 204, 207, 1061, 1802, 2218, 3963, 3966, 4062
GsaI	CCCAGC	1 cut(s)	2907
GsuI	CTGGAG	5 cut(s)	744, 2034, 2969, 3827, 3936
HapII	CCGG	6 cut(s)	1005, 2665, 3473, 3787, 4044, 4083
HgaI	GACGC	1 cut(s)	4345
HhaI	GCGC	1 cut(s)	652
Hin1I	GRCGYC	1 cut(s)	2390
Hin6I	GCGC	1 cut(s)	650
HinP1I	GCGC	1 cut(s)	650
HincII	GTYRAC	1 cut(s)	1231
HindII	GTYRAC	1 cut(s)	1231
HindIII	AAGCTT	3 cut(s)	102, 2006, 2925
HpaII	CCGG	6 cut(s)	1005, 2665, 3473, 3787, 4044, 4083
Hpy99I	CGWCG	2 cut(s)	1126, 1671
HpyCH4IV	ACGT	4 cut(s)	1102, 1121, 1666, 2390
HpySE526I	ACGT	4 cut(s)	1102, 1121, 1666, 2390
Hsp92I	GRCGYC	1 cut(s)	2390
HspAI	GCGC	1 cut(s)	650
KroI	GCCGGC	1 cut(s)	3472
KroNI	GCCGGC	1 cut(s)	3474
Ksp22I	TGATCA	2 cut(s)	853, 4234
LguI	GCTCTTC	1 cut(s)	3462
Lsp1109I	GCAGC	7 cut(s)	190, 193, 1047, 1813, 2229, 3974, 4048
MaeI	CTAG	3 cut(s)	1053, 3291, 3374
MaeII	ACGT	4 cut(s)	1102, 1121, 1666, 2390
MaeIII	GTNAC	7 cut(s)	43, 539, 1348, 1736, 3237, 3442, 4333
MbiI	CCGCTC	1 cut(s)	744
MfeI	CAATTG	2 cut(s)	609, 4119
MflI	RGATCY	6 cut(s)	5, 531, 2307, 2353, 2435, 4465
MhlI	GDGCHC	3 cut(s)	554, 4090, 4543
MlsI	TGGCCA	1 cut(s)	3994
MluI	ACGCGT	1 cut(s)	197
MluNI	TGGCCA	1 cut(s)	3994
MlyI	GAGTC	6 cut(s)	260, 355, 908, 1286, 3515, 4621
MmeI	TCCRAC	4 cut(s)	961, 1290, 3514, 4649
Mox20I	TGGCCA	1 cut(s)	3994
Mph1103I	ATGCAT	1 cut(s)	4008
MroNI	GCCGGC	1 cut(s)	3472
MroXI	GAANNNNTTC	4 cut(s)	2408, 3327, 3713, 3978
MscI	TGGCCA	1 cut(s)	3994
MslI	CAYNNNNRTG	6 cut(s)	41, 665, 1259, 2841, 3207, 3254
Msp20I	TGGCCA	1 cut(s)	3994
MspA1I	CMGCKG	2 cut(s)	1804, 4136
MspCI	CTTAAG	1 cut(s)	3230
MspI	CCGG	6 cut(s)	1005, 2665, 3473, 3787, 4044, 4083
MspR9I	CCNGG	9 cut(s)	1561, 2067, 2999, 3654, 3692, 3806, 4045, 4299, 4511
MunI	CAATTG	2 cut(s)	609, 4119
Mva1269I	GAATGC	3 cut(s)	1079, 2587, 3761
MvaI	CCWGG	8 cut(s)	1561, 2067, 2999, 3654, 3692, 3806, 4299, 4511
MvnI	CGCG	2 cut(s)	199, 652
NaeI	GCCGGC	1 cut(s)	3474
NciI	CCSGG	1 cut(s)	4045
NcoI	CCATGG	6 cut(s)	910, 1014, 1729, 2413, 2555, 4370
NgoMIV	GCCGGC	1 cut(s)	3472
NlaIV	GGNNCC	7 cut(s)	7, 330, 1449, 1883, 1962, 2244, 4087
NmuCI	GTSAC	2 cut(s)	43, 4333
NsiI	ATGCAT	1 cut(s)	4008
NspI	RCATGY	6 cut(s)	213, 1262, 2211, 2288, 2383, 4010
NspV	TTCGAA	3 cut(s)	1546, 3010, 4069
OliI	CACNNNNGTG	2 cut(s)	41, 1259
PaeI	GCATGC	2 cut(s)	2211, 2288
PaeR7I	CTCGAG	1 cut(s)	1294
PagI	TCATGA	1 cut(s)	2836
PciI	ACATGT	2 cut(s)	1258, 2379
PciSI	GCTCTTC	1 cut(s)	3462
PctI	GAATGC	3 cut(s)	1079, 2587, 3761
PdiI	GCCGGC	1 cut(s)	3474
PdmI	GAANNNNTTC	4 cut(s)	2408, 3327, 3713, 3978
PfeI	GAWTC	8 cut(s)	516, 2057, 2911, 3287, 3356, 3714, 4206, 4285
PflFI	GACNNNGTC	1 cut(s)	4184
PflMI	CCANNNNNTGG	3 cut(s)	611, 2947, 3729
PfoI	TCCNGGA	2 cut(s)	3804, 4043
PinAI	ACCGGT	2 cut(s)	1004, 4082
PkrI	GCNGC	9 cut(s)	13, 205, 208, 1062, 1803, 2219, 3964, 3967, 4063
PleI	GAGTC	6 cut(s)	259, 355, 907, 1286, 3514, 4621
PpsI	GAGTC	6 cut(s)	259, 355, 907, 1286, 3514, 4621
PpuMI	RGGWCCY	3 cut(s)	328, 1961, 4315
PscI	ACATGT	2 cut(s)	1258, 2379
PshBI	ATTAAT	3 cut(s)	1146, 3522, 3672
PsiI	TTATAA	1 cut(s)	431
Psp5II	RGGWCCY	3 cut(s)	328, 1961, 4315
Psp6I	CCWGG	8 cut(s)	1559, 2065, 2997, 3652, 3690, 3804, 4297, 4509
PspFI	CCCAGC	1 cut(s)	2903
PspGI	CCWGG	8 cut(s)	1559, 2065, 2997, 3652, 3690, 3804, 4297, 4509
PspN4I	GGNNCC	7 cut(s)	7, 330, 1449, 1883, 1962, 2244, 4087
PspPI	GGNCC	7 cut(s)	328, 1726, 1961, 2113, 3680, 4315, 4414
PspPPI	RGGWCCY	3 cut(s)	328, 1961, 4315
PstI	CTGCAG	1 cut(s)	2188
PstNI	CAGNNNCTG	1 cut(s)	1760
PsuI	RGATCY	6 cut(s)	5, 531, 2307, 2353, 2435, 4465
PsyI	GACNNNGTC	1 cut(s)	4184
PvuII	CAGCTG	2 cut(s)	1804, 4136
RseI	CAYNNNNRTG	6 cut(s)	41, 665, 1259, 2841, 3207, 3254
SapI	GCTCTTC	1 cut(s)	3462
SatI	GCNGC	9 cut(s)	12, 204, 207, 1061, 1802, 2218, 3963, 3966, 4062
Sau96I	GGNCC	7 cut(s)	328, 1726, 1961, 2113, 3680, 4315, 4414
ScaI	AGTACT	2 cut(s)	463, 4075
SchI	GAGTC	6 cut(s)	260, 355, 908, 1286, 3515, 4621
ScrFI	CCNGG	9 cut(s)	1561, 2067, 2999, 3654, 3692, 3806, 4045, 4299, 4511
SduI	GDGCHC	3 cut(s)	554, 4090, 4543
SfcI	CTRYAG	4 cut(s)	700, 2184, 3079, 4137
Sfr274I	CTCGAG	1 cut(s)	1294
SfuI	TTCGAA	3 cut(s)	1546, 3010, 4069
SinI	GGWCC	5 cut(s)	328, 1726, 1961, 2113, 4315
SlaI	CTCGAG	1 cut(s)	1294
SmiMI	CAYNNNNRTG	6 cut(s)	41, 665, 1259, 2841, 3207, 3254
SmlI	CTYRAG	7 cut(s)	404, 1294, 1655, 3230, 3875, 4548, 4655
SmoI	CTYRAG	7 cut(s)	404, 1294, 1655, 3230, 3875, 4548, 4655
SphI	GCATGC	2 cut(s)	2211, 2288
SsiI	CCGC	4 cut(s)	9, 12, 744, 3966
SspI	AATATT	3 cut(s)	1187, 1797, 2651
SspMI	CTAG	3 cut(s)	1053, 3291, 3374
StyD4I	CCNGG	9 cut(s)	1559, 2065, 2997, 3652, 3690, 3804, 4043, 4297, 4509
StyI	CCWWGG	9 cut(s)	910, 1014, 1729, 1946, 2413, 2555, 2769, 3510, 4370
TaiI	ACGT	4 cut(s)	1105, 1124, 1669, 2393
TaqII	GACCGA	1 cut(s)	2705
TatI	WGTACW	5 cut(s)	461, 1281, 1780, 2376, 4073
TauI	GCSGC	2 cut(s)	14, 3968
TfiI	GAWTC	8 cut(s)	516, 2057, 2911, 3287, 3356, 3714, 4206, 4285
TseFI	GTSAC	2 cut(s)	43, 4333
TseI	GCWGC	7 cut(s)	203, 206, 1060, 1801, 2217, 3962, 4061
Tsp45I	GTSAC	2 cut(s)	43, 4333
TspGWI	ACGGA	3 cut(s)	1226, 3842, 4085
Tth111I	GACNNNGTC	1 cut(s)	4184
Van91I	CCANNNNNTGG	3 cut(s)	611, 2947, 3729
Vha464I	CTTAAG	1 cut(s)	3230
VneI	GTGCAC	1 cut(s)	4539
VpaK11BI	GGWCC	5 cut(s)	328, 1726, 1961, 2113, 4315
VspI	ATTAAT	3 cut(s)	1146, 3522, 3672
XagI	CCTNNNNNAGG	3 cut(s)	2679, 3219, 4322
XbaI	TCTAGA	1 cut(s)	3290
XceI	RCATGY	6 cut(s)	213, 1262, 2211, 2288, 2383, 4010
XcmI	CCANNNNNNNNNTGG	3 cut(s)	3698, 4162, 4305
XhoI	CTCGAG	1 cut(s)	1294
XmiI	GTMKAC	4 cut(s)	2811, 2993, 3075, 3663
XmnI	GAANNNNTTC	4 cut(s)	2408, 3327, 3713, 3978
XspI	CTAG	3 cut(s)	1053, 3291, 3374
ZraI	GACGTC	1 cut(s)	2391
ZrmI	AGTACT	2 cut(s)	463, 4075
Zsp2I	ATGCAT	1 cut(s)	4008

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.