Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb1

Orthogroup ID

OG0002932

Physical Location & Seq

Genomic Coordinates Forward (+)

5615836 .. 5621628

Gene Structure 5793 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_1g10300.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4698 bp

ATGGGATCCATCGCGGCAGCCGATGATGCCGCCGCCGCCGCCGCAGCCACCGCACCTCTAAACGACGCCGAATCGGCTCCTTCTGCGCTTTCCTCAGGAACAACGAAGAAGAAGGGTAGAAAAGCTACCCAAAAGGAAGCGCCTGCTGGCGGTAGAGGTAGGAAGCGAAATGTGCCTCCAACTGGTGAAGAGCAATCCCAGTCCCGCAAAATGCCCAAGCGAGCTGCTGCGTGTAAAGACTTTAAGGAGAGGGCTGTTCGAGTTTCCGAAAAGTCTGCTTTCATCGAAAGCAAGGAGGAGCAGGTTGTGCCAGATGAAAGTCTTGCCGTAGTCATGACTTGTGGAAAGGATCCAGATGATGAGAAGCCGAACAGGAGATTGACTCAGTTTATCTTGCATAATGAAGATGGTATTGTACAGCCACTTGAGATGGTGCAACATGGTGATCTGTTTATTACTGGTACTCTATTGCCTCTTGATGCAAGTTCTGACAATGGCAAGGGCAAGGGAAAGGAAAAGGGTGTTATATGTGAAGGTTTTGGGCGGATAGAAGCTTGGGACATCTCTGGTTATGATGAGGGATATCCAATAATATGGCTTACAACTGATGTTGCTGATTATTGTTGTGTTAAGCCTGCAAGCAGCTACAAGAAACACTATGATAATTTCTGTGAGAAAGCACGTGCTTGCATAGAGGTCTACAAAGTACTGTCAAAATGCAACTCTGACTGCAGTCTTGATGAATTACTTGCTGGCATCGCTCGATCAATGAGTGGAAACAAATTCTTCTCTGGCAGTGCTTCTATCAAAGAATTTATCATTTCTCAAGGCGTGTTTATATATAATCAATTGGAAGGTTTGGAGGAGTCTTCTAAGAAGAATGATCGGGTATTGGCACAGCTGCCAGTTCTTGCAGCCCTTAGACAGGAGTGTATAATGCAGGGATACTATGTGACTTCAAACCCAGCAGCTTCAAGCGGTACATTAAAGATTGCTTCAGATGGAGGGAATAGTTCATCAAAAGTTGAAACCGAGGAAGACGAAGATGCCAAGTTGGCTAGACTTATTCAAGAAGAAGAATACTTTAAATCTATGAAGCAGAAAAAGCGTCAGGGTGCTGCCTCCATGTCAAAGAAATTTTACATCAAAATTAATGAAGATGAAATTGCTAATGATTATCCTGAACCTGCCTATTATAAGACTGCTATTGAAGAGACAGATGAATTTATAGTGGTTGAAAGTGAGGATGGGGATCTTCCCACGCACATGCTTCACAACTGGTGTCTATACAATTCAGACTCGAGACTGATTTCGCTGGAGCTTCTTCCAATGAAGGCTTGTGCTGATATTGATGTTACGATTTTTGGTTCTGGGGTTATGAGTGAAGATGATGGAAGCTGTGGATTTGATCTTGACAGTATCCAGTCGTCTTCAAGTGGTCCAGTAGTACCAGTAGCAGAGGATGCTTATGGCATGCCAGCTTACTTGAGTTCCATAAAGGAATGGGTGATAGAATGGGGAGCCTCAATGATTTCAGTATCAATTCGAACAGAACTGGCTTGGTACAGACTTGGCAAGCCATCAAAGCAGTATGCTAAGTGGTATGCACCAATACTGAAAACAGCAAGGGTTGGGAGAAGTATAATCACAATGCTCAAGGCAGAAAGTCGAGTAGCACGGCTCTCTTTTGCAGATGTCATTAAAAGATTGTCAGAGTTCCCTAACAGCGATGGTGGTTACATTTCTTCTGATCCAGCATCTGTTGAGAGATATGTGGTTGTACATGGACCGATATTACTTCAGCTGTTTACAGAATTTCCAGATGGGAAGATTAAAACTTGTCCATTTGTTCATGGTCTTAGAGACAAAATGGAGAAGAGGCACCATACTAAATGGGAAGTAAATAAGAAGATTTTGCAGAAGAGTGAATCAAATTTGAACCCGAGGGCATCAATGGGACCTGTGGCGTCCAAGAGGAAAGCAATGCAAGCTACGACAACAAAGCTGATTAACAGAATCTGGGGGGAGTACTATTCAAATTACTCTCCAGAAAATTCAAGCCCAGTGGAAACTTGTGAAAAGATAGAGGAGGATGAAGTTGATGAAGAGGTCCAGGAGGACTTAGAAGAGGATGATGCTGAAGAGAATTCATCAGTAGTGAAAGAAGCCCAAAGACCTTCTCCGATTTTAAGAAAAATTAAATCAAGCTCCAGTAGCAAGAAGGTTTTGTGGGATGAGGAGCCTGTGGGAAAAACATCTTCTGGTGAAGCTGTCTACAAGCATGCTGTTCTTCGTGGAGAAGAGATTTCTGTGGGTGGTGCTGTTATGGTGGAAGTTGATGGTTCAGATGAACTTCCTGTTATCTATTTTGTAGAATACATGTATGAATCATCAGATGGAGAAAAAATGCTCCATGGCAGAATGATGCAGCGAGGATCTGATACTGTTCTGGGAAACACTGCTAATGAGAGGGAGGTATTCTTGACAAATGAGTGCATAAACTTGGCACTGAAGGATGTGAAACAGACGGTTGTTGTGGGTATCAAGTCAATGCTGTGGGGGCATAAACATCGGAAGGAAAATGCTGATGCTGAGAGAATTGATAGAGCAAATGCAGAAGAGAGGAAGAAGAAAGGATTGCCGACTGAATACTACTGTAAAAGCTTGTATTTCCCGGAGAAAGGTGCTTTCCTTAGTCTTTCTCGTGATACTATTGGTCTGGGGTCTGGTTTTTGCTTCTCTTGCAAAGCTAATGAAGAAGAAAAGGCCAAAGAAGTCTTTGAAGTGAATTCATCAAAGACTGGTTTTATATACAGTGGAGTTGAGTACTCAGTTCATGATTATGTCTACGTAAGCCCCTATCATTTCTCTGTGCAAACAATTGAAACTGAACTTTTCAAGGCTGGACGGAACCTGGGGTTGAAACCTTTTGTTGTGTGCCAAGTGCTGGGGATAATTACAAAGGGATCTAAACAATCTGAAATGAAGTCTACCCAGGTTAAAGTTCGAAGGTTTTTCAGACCAGAGGACATTTCGGTTGAGAAAGCGTACTGCTCAGATGTCAGAGAAGTCTACTACAGTGAAGAACTACACATTTTGCCTGTAGATAGCATAGAAGGGAAATGTGAAGTCAGAAGGAGGAGTGATCTTCCAGAATGTAATGCTCCTGCATTGTTTCAGCATATATTCTTCTGTGAGCATTTATATGATCCTTCCAACGGGTCTCTTAAGCAGTTGCCGGCTAACATCAGGGTAAAGTACTCAACAGTAGGTGGTGATACTGAATCGAGAAAGAGAAAGGGCAAGTGTAAAGAGGGAGAAGATGTTCCTGAAGCTGAGCAACAGAGAGATGATTCTGAACACAAACGCCTAGCCACTTTGGATATATTTGCGGGATGTGGTGGCTTATCGGAGGGGTTGCATCAGGCTGGTGTGTCAATAACCAAGTGGGCAATCGAGTATGAAGAACCGGCTGGACAGGCTTTCCAACTCAACCATCCCGAGTCCAAGGTATTCATCAACAATTGTAACGTCATCTTGAAGGCCGTCATGGATAAGTGTGGAGATACGGATGATTGCATTTCCACTACTGATGCCAATGATTTAGCATCAGCACTAGATGAAAAGGAGAAGAGCGATTTGCCCTTGCCAGGACAGGTGGACTTTATAAATGGAGGCCCTCCATGCCAGGGTTTTTCTGGTATGAACAGATTTAACACCAGTACTTGGAGTAAAGTCCAGTGTGAAATGATACTGGCCTTCTTATCCTTTGCTGATTACTTCCGGCCAAAATACTTCCTCCTGGAGAATGTGAGAAACTTTGTGGCTTTCAATAAAGGACAGACATTCCGTCTGACTTTGGCTTCACTTCTGGAGATGGGTTATCAGGTGAGGTTTGGTATTCTGGAAGCCGGTGCTTATGGGGTTTCCCAGTCCCGAAAGCGAGCATTTATTTGGGCAGCTGCACCAGATGAGATTCTTCCCGAATGGCCAGAGCCAATGCATGTTTTTGGCGTGCCAGAGTTGAAGATCAACTTGTCCAGTAATTCATATTATGCTGCTGTTCGGAGTACTGCAAGCGGTGCCCCTTTCCGCCCCATAACAGTAAGAGACACAATTGGCGATCTCCCAGCTGTAGGGAATGGAGCATCCAAGGTCAATATGGAGTATGAAAGCGACCCTGTCTCATGGTTCCAGAAGAAAATCAGAGGGAACATGGCTGTTTTGACTGATCACATTTCAAAAGAAATGAACGAGCTGAACCTCATTCGGTGCAAGAGGATCCCCAAGAGGCCTGGTGCAGACTGGAAGGACCTTCCAGAGGAAAAAGTGAAGCTCTCTACTGGTCAGCTAGTCGACTTGATACCATGGTGCCTGCCCAACACCGCCAAACGCCACAATCAGTGGAAGGGCCTGTTCGGAAGGTTGGACTGGGAAGGCAACTTCCCAACCTCCATTACTGATCCTCAGCCGATGGGCAAGGTGGGAATGTGCTTCCATCCCGACCAGGACAGGATTATTACCGTTCGAGAATGTGCTCGTTCTCAAGGCTTCCCTGATAGCTACCAGTTTTATGGTAACACCCTCCACAAGCACAGGCAGATTGGAAATGCTGTCCCTCCAACTCTGGCCTACGCTCTCGGCCGAAAACTCAAGGAAGCAGTCAACAGCAAGAAGAGGTCTTCTTCACAAGAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000278 GO:0000281 GO:0000910 GO:0003006 GO:0003674 GO:0003824 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006349 GO:0006725 GO:0006807 GO:0007049 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008356 GO:0009292 GO:0009294 GO:0009790 GO:0009791 GO:0009793 GO:0009909 GO:0009910 GO:0009987 GO:0010069 GO:0010070 GO:0010154 GO:0010216 GO:0010424 GO:0010468 GO:0016740 GO:0016741 GO:0019222 GO:0022402 GO:0022414 GO:0032259 GO:0032501 GO:0032502 GO:0032776 GO:0034641 GO:0040029 GO:0043170 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044728 GO:0044764 GO:0046483 GO:0048316 GO:0048519 GO:0048580 GO:0048581 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0051093 GO:0051239 GO:0051241 GO:0051301 GO:0051704 GO:0060255 GO:0061458 GO:0061640 GO:0065007 GO:0071514 GO:0071704 GO:0090304 GO:1901360 GO:1903047 GO:2000026 GO:2000241 GO:2000242

KEGG Pathways

Metabolic & Signaling

ko00270 ko01100 ko05206 map00270 map01100 map05206

Pfam Domains

Protein Families

BAH DNA_methylase DNMT1-RFD

Protein Analysis

1566

Amino Acids

174.33

Weight (kDa)

5.79

Isoelectric Point (pI)

48.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DNMT1-RFD	PF12047	120 - 255	1.7e-33	Cytosine specific DNA methyltransferase replication foci domain
DNMT1-RFD	PF12047	418 - 572	1.1e-33	Cytosine specific DNA methyltransferase replication foci domain
BAH	PF01426	768 - 900	2.2e-20	BAH domain
BAH	PF01426	942 - 1056	1.8e-17	BAH domain
DNA_methylase	PF00145	1088 - 1515	6.6e-49	C-5 cytosine-specific DNA methylase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002932)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G08990 AT4G14140 AT4G14140 AT4G14140 AT5G49160 AT5G49160
fragaria_vesca	FvH4_1g10300 FvH4_1g10300
malus_domestica	MD02G1115100.v1.1 MD15G1231100.v1.1
prunus_persica	Prupe.7G060100_v2.0.a1 Prupe.7G183100_v2.0.a1 Prupe.7G183100_v2.0.a1
pyrus_communis	pycom02g09000 pycom02g09010 pycom15g20590 pycom15g20600
rosa_chinensis	RchiOBHm_Chr2g0097141 RchiOBHm_Chr5g0051581
rosa_laevigata	RLG00000016706 RLG00000034873
rosa_multiflora	Rmu_co8502141.1_g000001 Rmu_sc0001182.1_g000006 Rmu_ssc0000089.1_g000039
rosa_roxburghii	Rroxscaffold_1G00027090 Rroxscaffold_2G00144940
rosa_rugosa	Rorug02G0064600 Rorug05G0279000
rosa_samantha	Rh2AG111700 Rh2BG113700 Rh2CG116000 Rh2DG115000 Rh5AG350100 Rh5BG361900 Rh5CG386800 Rh5DG376800
rosa_wichuraiana	Rw2G008690 Rw5G033130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1197, 3668
Acc36I	ACCTGC	2 cut(s)	292, 1195
AccB1I	GGYRCC	3 cut(s)	1881, 4082, 4371
AccB7I	CCANNNNNTGG	2 cut(s)	2947, 3726
AccI	GTMKAC	6 cut(s)	699, 2274, 2847, 2990, 3072, 4356
AccII	CGCG	1 cut(s)	14
AcoI	YGGCCR	3 cut(s)	3785, 3989, 4642
AcsI	RAATTY	9 cut(s)	782, 812, 1136, 1223, 1814, 1933, 2053, 2146, 2788
AcuI	CTGAAG	5 cut(s)	981, 1784, 2160, 2531, 3351
AcvI	CACGTG	1 cut(s)	683
AcyI	GRCGYC	2 cut(s)	66, 1967
AflII	CTTAAG	1 cut(s)	3227
AflIII	ACRYGT	1 cut(s)	2379
AhdI	GACNNNNNGTC	1 cut(s)	3849
AjnI	CCWGG	8 cut(s)	2112, 2913, 2994, 3649, 3687, 3801, 4294, 4506
AloI	GAACNNNNNNTCC	2 cut(s)	3309, 3341
Alw21I	GWGCWC	1 cut(s)	4540
Alw26I	GTCTC	6 cut(s)	1208, 1297, 1857, 3228, 4104, 4189
AlwNI	CAGNNNCTG	2 cut(s)	1760, 2019
Ama87I	CYCGRG	3 cut(s)	1300, 1942, 3500
ApoI	RAATTY	9 cut(s)	782, 812, 1136, 1223, 1814, 1933, 2053, 2146, 2788
AseI	ATTAAT	1 cut(s)	1152
Asp700I	GAANNNNTTC	1 cut(s)	3324
AspLEI	GCGC	2 cut(s)	88, 142
AspS9I	GGNCC	7 cut(s)	1439, 1787, 1958, 2110, 3677, 4312, 4411
AsuC2I	CCSGG	1 cut(s)	2675
AsuHPI	GGTGA	6 cut(s)	197, 455, 1519, 2276, 3287, 3901
AsuII	TTCGAA	2 cut(s)	1546, 3007
AvaI	CYCGRG	3 cut(s)	1300, 1942, 3500
AvaII	GGWCC	5 cut(s)	1439, 1787, 1958, 2110, 4312
AxyI	CCTNAGG	1 cut(s)	94
BaeGI	GKGCMC	1 cut(s)	4087
BaeI	ACNNNNGTAYC	2 cut(s)	4355, 4388
BalI	TGGCCA	1 cut(s)	3991
BamHI	GGATCC	3 cut(s)	5, 349, 4281
BanI	GGYRCC	3 cut(s)	1881, 4082, 4371
BauI	CACGAG	1 cut(s)	2703
BbrPI	CACGTG	1 cut(s)	683
BbsI	GAAGAC	4 cut(s)	861, 1044, 1422, 4674
Bbv12I	GWGCWC	1 cut(s)	4540
BbvCI	CCTCAGC	1 cut(s)	4467
BceAI	ACGGC	3 cut(s)	311, 1694, 3530
BcgI	CGANNNNNNTGC	4 cut(s)	257, 291, 2552, 2586
BciT130I	CCWGG	8 cut(s)	2114, 2915, 2996, 3651, 3689, 3803, 4296, 4508
BciVI	GTATCC	2 cut(s)	938, 1430
BclI	TGATCA	1 cut(s)	4231
BcnI	CCSGG	1 cut(s)	2675
BcoDI	GTCTC	6 cut(s)	1208, 1297, 1857, 3228, 4104, 4189
BfaI	CTAG	4 cut(s)	1059, 3371, 3617, 4352
BfmI	CTRYAG	4 cut(s)	730, 3076, 3102, 4134
BfoI	RGCGCY	1 cut(s)	143
BfrI	CTTAAG	1 cut(s)	3227
BfuAI	ACCTGC	2 cut(s)	292, 1195
BfuI	GTATCC	2 cut(s)	938, 1430
BglI	GCCNNNNNGGC	2 cut(s)	74, 1055
BlpI	GCTNAGC	1 cut(s)	3336
BmcAI	AGTACT	6 cut(s)	708, 2030, 2828, 3260, 3724, 4072
Bme1390I	CCNGG	9 cut(s)	2114, 2675, 2915, 2996, 3651, 3689, 3803, 4296, 4508
Bme18I	GGWCC	5 cut(s)	1439, 1787, 1958, 2110, 4312
BmeRI	GACNNNNNGTC	1 cut(s)	3849
BmeT110I	CYCGRG	3 cut(s)	1300, 1942, 3500
BmgT120I	GGNCC	7 cut(s)	1439, 1787, 1958, 2110, 3677, 4312, 4411
BmrFI	CCNGG	9 cut(s)	2114, 2675, 2915, 2996, 3651, 3689, 3803, 4296, 4508
BmrI	ACTGGG	4 cut(s)	193, 2057, 3925, 4441
BmuI	ACTGGG	4 cut(s)	193, 2057, 3925, 4441
BoxI	GACNNNNGTC	1 cut(s)	732
BpiI	GAAGAC	4 cut(s)	861, 1044, 1422, 4674
BplI	GAGNNNNNCTC	2 cut(s)	367, 399
BpmI	CTGGAG	5 cut(s)	1337, 2031, 2194, 3824, 3893
Bpu10I	CCTNAGC	1 cut(s)	4467
Bpu1102I	GCTNAGC	1 cut(s)	3336
Bpu14I	TTCGAA	2 cut(s)	1546, 3007
BpuEI	CTTGAG	6 cut(s)	446, 810, 1507, 1640, 4530, 4637
BpuMI	CCSGG	1 cut(s)	2675
BsaAI	YACGTR	2 cut(s)	683, 2851
BsaBI	GATNNNNATC	1 cut(s)	1251
BsaHI	GRCGYC	2 cut(s)	66, 1967
BsaI	GGTCTC	1 cut(s)	3228
BsaJI	CCNNGG	9 cut(s)	1032, 1943, 2413, 2914, 2994, 3507, 3688, 4152, 4367
BsaXI	ACNNNNNCTCC	2 cut(s)	3309, 3339
Bse118I	RCCGGY	3 cut(s)	3238, 3469, 3911
Bse21I	CCTNAGG	1 cut(s)	94
Bse3DI	GCAATG	1 cut(s)	1989
Bse8I	GATNNNNATC	1 cut(s)	1251
BseBI	CCWGG	8 cut(s)	2114, 2915, 2996, 3651, 3689, 3803, 4296, 4508
BseDI	CCNNGG	9 cut(s)	1032, 1943, 2413, 2914, 2994, 3507, 3688, 4152, 4367
BseJI	GATNNNNATC	1 cut(s)	1251
BseMI	GCAATG	1 cut(s)	1989
BseMII	CTCAG	7 cut(s)	108, 398, 2583, 2844, 3069, 3327, 4481
BseRI	GAGGAG	5 cut(s)	311, 878, 2102, 2252, 3154
BseSI	GKGCMC	1 cut(s)	4087
BseX3I	CGGCCG	1 cut(s)	4642
BseYI	CCCAGC	3 cut(s)	964, 2947, 4129
BsgI	GTGCAG	2 cut(s)	3948, 4320
Bsh1236I	CGCG	1 cut(s)	14
Bsh1285I	CGRYCG	1 cut(s)	4645
BshNI	GGYRCC	3 cut(s)	1881, 4082, 4371
BsiEI	CGRYCG	1 cut(s)	4645
BsiHKAI	GWGCWC	1 cut(s)	4540
BsiHKCI	CYCGRG	3 cut(s)	1300, 1942, 3500
BsiSI	CCGG	5 cut(s)	2675, 3239, 3470, 3784, 3912
BslFI	GGGAC	5 cut(s)	187, 572, 1971, 3919, 4601
BsmAI	GTCTC	6 cut(s)	1208, 1297, 1857, 3228, 4104, 4189
BsmFI	GGGAC	5 cut(s)	187, 572, 1971, 3919, 4601
Bso31I	GGTCTC	1 cut(s)	3228
BsoBI	CYCGRG	3 cut(s)	1300, 1942, 3500
Bsp119I	TTCGAA	2 cut(s)	1546, 3007
Bsp1286I	GDGCHC	2 cut(s)	4087, 4540
Bsp1407I	TGTACA	2 cut(s)	415, 1780
Bsp1720I	GCTNAGC	1 cut(s)	3336
Bsp19I	CCATGG	2 cut(s)	2413, 4367
BspCNI	CTCAG	7 cut(s)	107, 397, 2584, 2843, 3068, 3328, 4480
BspFNI	CGCG	1 cut(s)	14
BspHI	TCATGA	2 cut(s)	333, 2836
BspMAI	CTGCAG	1 cut(s)	734
BspMI	ACCTGC	2 cut(s)	292, 1195
BspQI	GCTCTTC	2 cut(s)	183, 3626
BspT104I	TTCGAA	2 cut(s)	1546, 3007
BspT107I	GGYRCC	3 cut(s)	1881, 4082, 4371
BspTI	CTTAAG	1 cut(s)	3227
BspTNI	GGTCTC	1 cut(s)	3228
BsrDI	GCAATG	1 cut(s)	1989
BsrFI	RCCGGY	3 cut(s)	3238, 3469, 3911
BsrGI	TGTACA	2 cut(s)	415, 1780
BssAI	RCCGGY	3 cut(s)	3238, 3469, 3911
BssECI	CCNNGG	9 cut(s)	1032, 1943, 2413, 2914, 2994, 3507, 3688, 4152, 4367
BssNI	GRCGYC	2 cut(s)	66, 1967
BssSI	CACGAG	1 cut(s)	2703
BssT1I	CCWWGG	4 cut(s)	2413, 3507, 4152, 4367
Bst2BI	CACGAG	1 cut(s)	2703
Bst2UI	CCWGG	8 cut(s)	2114, 2915, 2996, 3651, 3689, 3803, 4296, 4508
BstACI	GRCGYC	2 cut(s)	66, 1967
BstAFI	CTTAAG	1 cut(s)	3227
BstAPI	GCANNNNNTGC	3 cut(s)	307, 1463, 4082
BstAUI	TGTACA	2 cut(s)	415, 1780
BstBAI	YACGTR	2 cut(s)	683, 2851
BstBI	TTCGAA	2 cut(s)	1546, 3007
BstDSI	CCRYGG	2 cut(s)	2413, 4367
BstENI	CCTNNNNNAGG	2 cut(s)	923, 4319
BstFNI	CGCG	1 cut(s)	14
BstH2I	RGCGCY	1 cut(s)	143
BstHHI	GCGC	2 cut(s)	88, 142
BstMAI	GTCTC	6 cut(s)	1208, 1297, 1857, 3228, 4104, 4189
BstMCI	CGRYCG	1 cut(s)	4645
BstNI	CCWGG	8 cut(s)	2114, 2915, 2996, 3651, 3689, 3803, 4296, 4508
BstNSI	RCATGY	5 cut(s)	1270, 1477, 2285, 2383, 4007
BstPAI	GACNNNNGTC	1 cut(s)	732
BstSCI	CCNGG	9 cut(s)	2112, 2673, 2913, 2994, 3649, 3687, 3801, 4294, 4506
BstSFI	CTRYAG	4 cut(s)	730, 3076, 3102, 4134
BstSLI	GKGCMC	1 cut(s)	4087
BstSNI	TACGTA	1 cut(s)	2851
BstUI	CGCG	1 cut(s)	14
BstV2I	GAAGAC	4 cut(s)	861, 1044, 1422, 4674
BstX2I	RGATCY	6 cut(s)	5, 349, 1252, 2435, 2966, 4281
BstXI	CCANNNNNNTGG	2 cut(s)	594, 4574
BstYI	RGATCY	6 cut(s)	5, 349, 1252, 2435, 2966, 4281
BstZI	CGGCCG	1 cut(s)	4642
Bsu36I	CCTNAGG	1 cut(s)	94
BsuI	GTATCC	2 cut(s)	938, 1430
BtgI	CCRYGG	2 cut(s)	2413, 4367
BtgZI	GCGATG	2 cut(s)	742, 1743
BtsI	GCAGTG	2 cut(s)	802, 2457
BtsIMutI	CAGTG	8 cut(s)	802, 2070, 2457, 2507, 2821, 3085, 3746, 4409
BveI	ACCTGC	2 cut(s)	292, 1195
CaiI	CAGNNNCTG	2 cut(s)	1760, 2019
CciI	TCATGA	2 cut(s)	333, 2836
CfoI	GCGC	2 cut(s)	88, 142
Cfr10I	RCCGGY	3 cut(s)	3238, 3469, 3911
Cfr13I	GGNCC	7 cut(s)	1439, 1787, 1958, 2110, 3677, 4312, 4411
CseI	GACGC	3 cut(s)	74, 1097, 1956
CspCI	CAANNNNNGTGG	2 cut(s)	2046, 2081
DraI	TTTAAA	1 cut(s)	1087
DriI	GACNNNNNGTC	1 cut(s)	3849
EaeI	YGGCCR	3 cut(s)	3785, 3989, 4642
EagI	CGGCCG	1 cut(s)	4642
Eam1105I	GACNNNNNGTC	1 cut(s)	3849
EciI	GGCGGA	2 cut(s)	559, 4082
EclXI	CGGCCG	1 cut(s)	4642
Eco105I	TACGTA	1 cut(s)	2851
Eco130I	CCWWGG	4 cut(s)	2413, 3507, 4152, 4367
Eco147I	AGGCCT	1 cut(s)	4294
Eco31I	GGTCTC	1 cut(s)	3228
Eco32I	GATATC	1 cut(s)	584
Eco47I	GGWCC	5 cut(s)	1439, 1787, 1958, 2110, 4312
Eco52I	CGGCCG	1 cut(s)	4642
Eco57I	CTGAAG	5 cut(s)	981, 1784, 2160, 2531, 3351
Eco72I	CACGTG	1 cut(s)	683
Eco81I	CCTNAGG	1 cut(s)	94
Eco88I	CYCGRG	3 cut(s)	1300, 1942, 3500
EcoNI	CCTNNNNNAGG	2 cut(s)	923, 4319
EcoO109I	RGGNCCY	4 cut(s)	1958, 3677, 4312, 4411
EcoRI	GAATTC	2 cut(s)	2146, 2788
EcoRII	CCWGG	8 cut(s)	2112, 2913, 2994, 3649, 3687, 3801, 4294, 4506
EcoRV	GATATC	1 cut(s)	584
EcoT14I	CCWWGG	4 cut(s)	2413, 3507, 4152, 4367
EcoT22I	ATGCAT	1 cut(s)	4005
ErhI	CCWWGG	4 cut(s)	2413, 3507, 4152, 4367
FalI	AAGNNNNNCTT	2 cut(s)	4019, 4051
FaqI	GGGAC	5 cut(s)	187, 572, 1971, 3919, 4601
FauI	CCCGC	2 cut(s)	212, 3385
FbaI	TGATCA	1 cut(s)	4231
FblI	GTMKAC	6 cut(s)	699, 2274, 2847, 2990, 3072, 4356
FspBI	CTAG	4 cut(s)	1059, 3371, 3617, 4352
GlaI	GCGC	2 cut(s)	87, 141
GsaI	CCCAGC	3 cut(s)	968, 2951, 4133
GsuI	CTGGAG	5 cut(s)	1337, 2031, 2194, 3824, 3893
HaeII	RGCGCY	1 cut(s)	143
HapII	CCGG	5 cut(s)	2675, 3239, 3470, 3784, 3912
HgaI	GACGC	3 cut(s)	74, 1097, 1956
HhaI	GCGC	2 cut(s)	88, 142
Hin1I	GRCGYC	2 cut(s)	66, 1967
Hin6I	GCGC	2 cut(s)	86, 140
HinP1I	GCGC	2 cut(s)	86, 140
HincII	GTYRAC	2 cut(s)	4357, 4666
HindII	GTYRAC	2 cut(s)	4357, 4666
HindIII	AAGCTT	2 cut(s)	552, 2662
HpaII	CCGG	5 cut(s)	2675, 3239, 3470, 3784, 3912
HphI	GGTGA	6 cut(s)	197, 455, 1519, 2276, 3287, 3901
Hpy166II	GTNNAC	9 cut(s)	700, 1809, 2275, 2848, 2991, 3073, 3661, 4357, 4666
Hpy8I	GTNNAC	9 cut(s)	700, 1809, 2275, 2848, 2991, 3073, 3661, 4357, 4666
Hpy99I	CGWCG	1 cut(s)	68
HpyCH4IV	ACGT	3 cut(s)	682, 2850, 3531
HpySE526I	ACGT	3 cut(s)	682, 2850, 3531
Hsp92I	GRCGYC	2 cut(s)	66, 1967
HspAI	GCGC	2 cut(s)	86, 140
KroI	GCCGGC	1 cut(s)	3238
KroNI	GCCGGC	1 cut(s)	3240
Ksp22I	TGATCA	1 cut(s)	4231
LguI	GCTCTTC	2 cut(s)	183, 3626
LmnI	GCTCC	9 cut(s)	82, 298, 1318, 1520, 2213, 2239, 2415, 3169, 4145
MaeI	CTAG	4 cut(s)	1059, 3371, 3617, 4352
MaeII	ACGT	3 cut(s)	682, 2850, 3531
MaeIII	GTNAC	5 cut(s)	952, 1354, 1736, 3527, 4577
MfeI	CAATTG	4 cut(s)	848, 2880, 3523, 4116
MflI	RGATCY	6 cut(s)	5, 349, 1252, 2435, 2966, 4281
MhlI	GDGCHC	2 cut(s)	4087, 4540
MlsI	TGGCCA	1 cut(s)	3991
MluNI	TGGCCA	1 cut(s)	3991
MlyI	GAGTC	4 cut(s)	376, 875, 1292, 3512
MmeI	TCCRAC	5 cut(s)	203, 3240, 3511, 4407, 4646
Mox20I	TGGCCA	1 cut(s)	3991
Mph1103I	ATGCAT	1 cut(s)	4005
MroNI	GCCGGC	1 cut(s)	3238
MroXI	GAANNNNTTC	1 cut(s)	3324
MscI	TGGCCA	1 cut(s)	3991
MslI	CAYNNNNRTG	3 cut(s)	1265, 2841, 3204
Msp20I	TGGCCA	1 cut(s)	3991
MspA1I	CMGCKG	4 cut(s)	901, 1804, 3962, 4133
MspCI	CTTAAG	1 cut(s)	3227
MspI	CCGG	5 cut(s)	2675, 3239, 3470, 3784, 3912
MspR9I	CCNGG	9 cut(s)	2114, 2675, 2915, 2996, 3651, 3689, 3803, 4296, 4508
MunI	CAATTG	4 cut(s)	848, 2880, 3523, 4116
MvaI	CCWGG	8 cut(s)	2114, 2915, 2996, 3651, 3689, 3803, 4296, 4508
MvnI	CGCG	1 cut(s)	14
NaeI	GCCGGC	1 cut(s)	3240
NciI	CCSGG	1 cut(s)	2675
NcoI	CCATGG	2 cut(s)	2413, 4367
NgoMIV	GCCGGC	1 cut(s)	3238
NmeAIII	GCCGAG	1 cut(s)	4620
NmuCI	GTSAC	1 cut(s)	952
NsiI	ATGCAT	1 cut(s)	4005
NspI	RCATGY	5 cut(s)	1270, 1477, 2285, 2383, 4007
NspV	TTCGAA	2 cut(s)	1546, 3007
PaeI	GCATGC	2 cut(s)	1477, 2285
PaeR7I	CTCGAG	1 cut(s)	1300
PagI	TCATGA	2 cut(s)	333, 2836
PceI	AGGCCT	1 cut(s)	4294
PciI	ACATGT	1 cut(s)	2379
PciSI	GCTCTTC	2 cut(s)	183, 3626
PcsI	WCGNNNNNNNCGW	1 cut(s)	18
PdiI	GCCGGC	1 cut(s)	3240
PdmI	GAANNNNTTC	1 cut(s)	3324
PfeI	GAWTC	7 cut(s)	71, 1928, 2016, 2387, 3284, 3353, 3976
PflFI	GACNNNGTC	1 cut(s)	4181
PflMI	CCANNNNNTGG	2 cut(s)	2947, 3726
PfoI	TCCNGGA	3 cut(s)	2112, 2673, 3801
PleI	GAGTC	4 cut(s)	376, 874, 1292, 3511
PmaCI	CACGTG	1 cut(s)	683
PmlI	CACGTG	1 cut(s)	683
PpsI	GAGTC	4 cut(s)	376, 874, 1292, 3511
Ppu21I	YACGTR	2 cut(s)	683, 2851
PpuMI	RGGWCCY	2 cut(s)	1958, 4312
PscI	ACATGT	1 cut(s)	2379
PshAI	GACNNNNGTC	1 cut(s)	732
PshBI	ATTAAT	1 cut(s)	1152
PsiI	TTATAA	2 cut(s)	1197, 3668
Psp5II	RGGWCCY	2 cut(s)	1958, 4312
Psp6I	CCWGG	8 cut(s)	2112, 2913, 2994, 3649, 3687, 3801, 4294, 4506
PspCI	CACGTG	1 cut(s)	683
PspFI	CCCAGC	3 cut(s)	964, 2947, 4129
PspGI	CCWGG	8 cut(s)	2112, 2913, 2994, 3649, 3687, 3801, 4294, 4506
PspPI	GGNCC	7 cut(s)	1439, 1787, 1958, 2110, 3677, 4312, 4411
PspPPI	RGGWCCY	2 cut(s)	1958, 4312
PstI	CTGCAG	1 cut(s)	734
PstNI	CAGNNNCTG	2 cut(s)	1760, 2019
PsuI	RGATCY	6 cut(s)	5, 349, 1252, 2435, 2966, 4281
PsyI	GACNNNGTC	1 cut(s)	4181
PvuII	CAGCTG	4 cut(s)	901, 1804, 3962, 4133
RseI	CAYNNNNRTG	3 cut(s)	1265, 2841, 3204
SalI	GTCGAC	1 cut(s)	4355
SapI	GCTCTTC	2 cut(s)	183, 3626
Sau96I	GGNCC	7 cut(s)	1439, 1787, 1958, 2110, 3677, 4312, 4411
ScaI	AGTACT	6 cut(s)	708, 2030, 2828, 3260, 3724, 4072
SchI	GAGTC	4 cut(s)	376, 875, 1292, 3512
ScrFI	CCNGG	9 cut(s)	2114, 2675, 2915, 2996, 3651, 3689, 3803, 4296, 4508
SduI	GDGCHC	2 cut(s)	4087, 4540
SfcI	CTRYAG	4 cut(s)	730, 3076, 3102, 4134
Sfr274I	CTCGAG	1 cut(s)	1300
SfuI	TTCGAA	2 cut(s)	1546, 3007
SinI	GGWCC	5 cut(s)	1439, 1787, 1958, 2110, 4312
SlaI	CTCGAG	1 cut(s)	1300
SmiMI	CAYNNNNRTG	3 cut(s)	1265, 2841, 3204
SmlI	CTYRAG	8 cut(s)	425, 825, 1300, 1486, 1655, 3227, 4545, 4652
SmoI	CTYRAG	8 cut(s)	425, 825, 1300, 1486, 1655, 3227, 4545, 4652
SnaBI	TACGTA	1 cut(s)	2851
SphI	GCATGC	2 cut(s)	1477, 2285
SseBI	AGGCCT	1 cut(s)	4294
SspMI	CTAG	4 cut(s)	1059, 3371, 3617, 4352
StuI	AGGCCT	1 cut(s)	4294
StyD4I	CCNGG	9 cut(s)	2112, 2673, 2913, 2994, 3649, 3687, 3801, 4294, 4506
StyI	CCWWGG	4 cut(s)	2413, 3507, 4152, 4367
TaiI	ACGT	3 cut(s)	685, 2853, 3534
TaqII	GACCGA	1 cut(s)	1804
TatI	WGTACW	8 cut(s)	415, 706, 1780, 2028, 2826, 3258, 3722, 4070
TauI	GCSGC	6 cut(s)	17, 32, 35, 38, 41, 44
TfiI	GAWTC	7 cut(s)	71, 1928, 2016, 2387, 3284, 3353, 3976
TscAI	CASTG	8 cut(s)	802, 2070, 2464, 2514, 2821, 3085, 3746, 4409
TseFI	GTSAC	1 cut(s)	952
Tsp45I	GTSAC	1 cut(s)	952
TspGWI	ACGGA	3 cut(s)	2923, 3584, 3839
TspRI	CASTG	8 cut(s)	802, 2070, 2464, 2514, 2821, 3085, 3746, 4409
Tth111I	GACNNNGTC	1 cut(s)	4181
Van91I	CCANNNNNTGG	2 cut(s)	2947, 3726
Vha464I	CTTAAG	1 cut(s)	3227
VpaK11BI	GGWCC	5 cut(s)	1439, 1787, 1958, 2110, 4312
VspI	ATTAAT	1 cut(s)	1152
XagI	CCTNNNNNAGG	2 cut(s)	923, 4319
XapI	RAATTY	9 cut(s)	782, 812, 1136, 1223, 1814, 1933, 2053, 2146, 2788
XceI	RCATGY	5 cut(s)	1270, 1477, 2285, 2383, 4007
XcmI	CCANNNNNNNNNTGG	2 cut(s)	3695, 4159
XhoI	CTCGAG	1 cut(s)	1300
XmiI	GTMKAC	6 cut(s)	699, 2274, 2847, 2990, 3072, 4356
XmnI	GAANNNNTTC	1 cut(s)	3324
XspI	CTAG	4 cut(s)	1059, 3371, 3617, 4352
ZrmI	AGTACT	6 cut(s)	708, 2030, 2828, 3260, 3724, 4072
Zsp2I	ATGCAT	1 cut(s)	4005

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.