Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0002932

Physical Location & Seq

Genomic Coordinates Reverse (-)

56188499 .. 56194374

Gene Structure 5876 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G033130.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4455 bp

ATGGGATCCGCCGCAGGATTAGAACCCGACCCTCCCACCAAAGTGACTAAACCAACATCTTCTTCGTCTTCCTCTGGAATGACAAAGAAGAAGGGCAAAAAAGCTTCTCAAAAGGAAGCACCTACTGGCCGTAGAGGTAGGAAGCGAAATTTGCCTCAGAGCGATGAAGAGCATGCCCAGTCTCGCAAAATGCCCAAGCGTGCTGCTGCATGTAAAGACTTTAAGGAGAAGTCTGTCCATATTACTGATGAGTCTGATCTCATCGCAAGCAAGGAGGAACAGATTGTGCCAGAAGAAGGTCTTGCTGTACGCTTGACTTGTGGCAAGGACCCAGATAATGAGAAGCCAAGCAGAAGACTGACTCAATTTATCTTGCATAGTGAAGATGGTACTGCAAGACCACTTGAGATGGTGGAAGCTGGTCTGTTTATAACTGGCACTATATTGCCTCTTCGTGAAAGTACTGAGAAGGACAAGGACAAGGACAGCAGTATTAAATGTGAAGGTTTTGGGCGAATCGATGCTTGGAAGATCTCTGGTTACGATGAGGGCTCTCCAATGATATGGCTTTCAACTAAAGTTGCCGATTATTGTTGTGTTAAACCAGCAATTGGCTACAAGAAGCACTATGATCTCTTCTTTGAGAAAGCGCGTGCTTGCATAGAGGTGTACCAGAAACTTTCAAAATGTAAATCTGGCTGTAGTCTTGAAGAATTACTTGCTGGAGTTGCTCGATCATTGAGCGGAAGCAAATTCTTTTCTGGCAGTGCATCTATCAAAGAATTTGTCCTATCTCACGGTGAGTTTATCTATAAACGATTGATAGGTTTGGAACAGTCATTGAAGAAGAATGATCAGATATTGGCACAGCTGCCTGTTCTCACTGCCCTCAGAGATGAGTCTATAAACTATGGATATTATGAGCAACCAGAACCAGTAGCTTCAAGTGCTCCCTTAAAGATTGCTTCAGATATCAGAGTTGGAGGGAATGAACTGAGAACAACCGGTTCATCCATAGTTGAAGCTGAAGAAGATGAAGATGCAAAGTTAGCTAGACTGCTGCAAGAAGAAGAATGCTTTAAGTCTATGAAACAAAGAAAACGTCAGGGTGCTACCTCCACGTCGAGTAAATTCTACATCAAAATTAATGAAGATGAAATTGCTAATGATTATCCTGAACCTAAATATTATGACAATTCCATTGAAGAGACGGATGAGTTTATTGTTGTTGACAGTGAGGCTGGTGATCTTCCCACACATGTGCTTGATAATTGGTGTCTGTACAACTTAGACTCGAGACTGATTTCGTTGGAGCTTCTTCCAATGACAGATTGCCCGGATATTGATGTTACAATTTTTGGGTCAGGGGTTATGAGTGAAGATGATGGAAGCTGTGGCTTTAATCTCGATACGGACAATGCTTATGGCATCCCAATTTATTTGAGTGCCATAAAGGAATGGGTGATAGAATGGGGAGCATCAATGATCTCAGTATCAATTCGAACAGAGCTGGCCTGGTACAGACTTGGCAAGCCATCGCAGCAGTATGCTTTGTGGTATGAACCAATACTGAAAACAGCAAGGGTTGGGAGAAGTATAATCACTATGCTCAAGGCTGAAAGTCGAGTAGCACGGCTTTCTTTTGCAGATGTCATTAAACGATTGTCAGAGTTCCCAAAGAACCATGGTGGTTACATTTCTCTGATCCAGCATCTGTTGAGAGATATGTGGTTGAAGGCAATGCAGGCTACAACAACAAAGCTTATCAACAGAATTTGGGGGGACTACTATTCAAATTACTCTCCAGTAGATTCAAGCCCAGGAGCTACTTGTGGAAAGGTAGAGGAGAATGAAGTTATAGAAGAGGTCCAAGAGGACATAGAAGAGTATGATGTAGATGAGAATTCATCAGTAATGGAACAAGCCCAAAGGTCTCCTGCAGTTTCAAGAAAGCCTAAAGCATGCTTCAGCAGCAGTGAGATTTTGTGGGGTGAGGAGCCTGTGGGAAAAACCTGTTCTGGTGAAGCTCTCTACAAGCATGCCGTTCTTCGTGGAGATGAGATCTCTGTGGGTGGTGCTGTTTTTGTGGAAATTGATGTATCAAAAGATCCTGTTATCTATCTTGTTGAGTACATGTTTGAGACGTTAGATGGAAAGAAGATGTTCCATGGGAGAATGATGCTGAGAGGATCTGAGACTGTTCTTGGAAACACTGCTAATGAGAGAGAAGTGTTTTTAACTGATGAGTGCGTGAACTTGGCATTGGAGGATGTGGAACAGACTGTTGTTGTGGGTATCCAATCAATGCCATGGGGGCATCAGTATAGGAAGAAGAATGCCGATACTAATAGAATTGATAGAGCAAGAGCAGAAGAGAGGAAGAAGCAAGGATTGCCAACAGAATATTACTGTAAAAGCCGGTATTGCCCTGAGAAAGGTGCTATCTTTAGTCTTTCTCATCATACTATCGGTCTGGGTTCTGGTTTTTGCCACTCTTGCAAAGTTAATGAAGATGAAAAGGCCATGGAAGTCTTTAAAGTGAATTCATCAAAGACTGGTTTTGTATACAGTGGAGCTGAGTATTTCATTCATGATTATGTCTATGTAAGCCCCTATCATTTTTCTGTGGAAAGGACTGGGACTGAGCTTTTCAAGGCTGGGAGGAATCTGGGATTGAAAGCTTATGTTGTGTGCCAAGTGCTGGACATAATTACTGCAAAGGAATATAAACAACCTGATATGAAGTCTACCCAGGTTAAAGTTCGAAGGTTTTTCAGACCAGAGGACATTTCAGTAGAGAAGGCATACTGCTCTGATGTCAGAGAGGTCTACTACAGTGAAGAAACACACATTATCCCTGCTGATAGCTTAGAAGGAAAATGTGAAGTTAGAAAGAGGAGTGATCTTCCAGAATGTGATGCTCCTGCAATGTTTGAGCATATTTTCTTTTGTGAGCATTTATATGATCCTTCTAAAGGGTCTCTTAAGCAGTTACCGGTTCACATCAAGGTGAGGCACTCAGCAGTTGGTGGTGATGCTGAATCTAGAAAGAAAAAGGGCAAATGCAAAGAAGGAGAAGATGTTCCAGAAGTTGAGAAACAGAGAGATGATTCTGAACACAAACGCCTAGCCACTTTGGATATATTTGCTGGATGTGGTGGCCTGTCAGAAGGTTTGCATCAGGCTGGTGTTTCAGTAACGAAGTGGGCAATCGAGTATGAAGAGCCGGCTGGACAGGCTTTCCAACTCAACCATCCTGAGTCCAAGGTGTTCATTAATAATTGCAATGTCATCTTGAAGGCCGTCATGGACAAATGTGGAGATATGGATGATTGCATCTCCACTACTGAAGCCAATGATTTAGCCTCGTCACTTGATGAGAAGGTGAAAGACAATTTGCCCTTGCCAGGGCAAGTAGACTTTATTAATGGAGGCCCTCCATGCCAGGGATTTTCTGGTATGAATAGATTCAATACCAGCACTTGGAGTAAAGTCCAGTGTGAAATGATATTGGCATTCTTATCCTTTGCTGATTACTTCCGGCCAAAATACTTCCTCCTGGAGAATGTGAGGAACTTTGTGGCTTTTAATAAAGGACAGACATTCCGTCTGACTGTGGCTTCACTTCTTGAGATGGGTTATCAGGTGAGGTTTGGTGTTCTGGAAGCTGGAGCTTATGGGGTTTCTCAATCCCGAAAGCGTGCATTTATCTGGGCAGCCGCACCAGAAGAGGTTCTTCCAGATTGGCCAGAGCCAATGCATGTTTTTGGTGTGCCAGAGTTGAAGATCAATTTATCCGGGAACTCCTATTATGCTGCTGTTCGAAGTACTGCAACCGGTGCCCCATTCCGTGCCATAACAGTAAGAGACACAATTGGCGATCTTCCTGCAGTAGGCAATGGAGCTTCCAAGATCAATTTGGAATATGAAAGCGACCCTGTCTCGTGGTTCCAGAAGAGAATCAGAGGGAACATGGCTGTTTTGACTGATCACATTTCGAAAGAAATGAACGAGCTGAACCTCATCCGATGTAAGAAGATTCCCAAGAGGCCAGGTGCAGATTGGCAGGACCTTCCTGAGGAAAAGGTGACACTCTCTACTGGTCAAATAGTGGACTTGATACCATGGTGCTTGCCCAACACAGCGAAACGCCACAACCAGTGGAAGGGCCTGTTTGGAAGGTTGGACTGGGAAGGCAACTTCCCAACCTCCATCACAGATCCTCAGCCAATGGGTAAGGTGGGAATGTGCTTTCATCCTGACCAGGACAGAATTCTGACAGTTAGGGAATGTGCTCGATCTCAAGGCTTTCCTGACAGCTACCAGTTTTACGGCAACATTCTTCACAAGCATAGGCAGATTGGAAATGCTGTTCCTCCGACTCTGGCTTATGCTCTGGGCAGAAAACTCAAGGAAGCAGTGGATAGCAAGAAGAAGTCTTCTTCACAAGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000278 GO:0000281 GO:0000910 GO:0003006 GO:0003674 GO:0003824 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006349 GO:0006725 GO:0006807 GO:0007049 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008356 GO:0009292 GO:0009294 GO:0009790 GO:0009791 GO:0009793 GO:0009909 GO:0009910 GO:0009987 GO:0010069 GO:0010070 GO:0010154 GO:0010216 GO:0010424 GO:0010468 GO:0016740 GO:0016741 GO:0019222 GO:0022402 GO:0022414 GO:0032259 GO:0032501 GO:0032502 GO:0032776 GO:0034641 GO:0040029 GO:0043170 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044728 GO:0044764 GO:0046483 GO:0048316 GO:0048519 GO:0048580 GO:0048581 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0051093 GO:0051239 GO:0051241 GO:0051301 GO:0051704 GO:0060255 GO:0061458 GO:0061640 GO:0065007 GO:0071514 GO:0071704 GO:0090304 GO:1901360 GO:1903047 GO:2000026 GO:2000241 GO:2000242

KEGG Pathways

Metabolic & Signaling

ko00270 ko01100 ko05206 map00270 map01100 map05206

Pfam Domains

Protein Families

BAH DNA_methylase DNMT1-RFD

Protein Analysis

1484

Amino Acids

166.31

Weight (kDa)

5.83

Isoelectric Point (pI)

46.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DNMT1-RFD	PF12047	113 - 245	3.4e-31	Cytosine specific DNA methyltransferase replication foci domain
DNMT1-RFD	PF12047	416 - 557	7.3e-32	Cytosine specific DNA methyltransferase replication foci domain
BAH	PF01426	687 - 814	6.9e-17	BAH domain
BAH	PF01426	861 - 999	6.6e-25	BAH domain
DNA_methylase	PF00145	1044 - 1471	1.4e-47	C-5 cytosine-specific DNA methylase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002932)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G08990 AT4G14140 AT4G14140 AT4G14140 AT5G49160 AT5G49160
fragaria_vesca	FvH4_1g10300 FvH4_1g10300
malus_domestica	MD02G1115100.v1.1 MD15G1231100.v1.1
prunus_persica	Prupe.7G060100_v2.0.a1 Prupe.7G183100_v2.0.a1 Prupe.7G183100_v2.0.a1
pyrus_communis	pycom02g09000 pycom02g09010 pycom15g20590 pycom15g20600
rosa_chinensis	RchiOBHm_Chr2g0097141 RchiOBHm_Chr5g0051581
rosa_laevigata	RLG00000016706 RLG00000034873
rosa_multiflora	Rmu_co8502141.1_g000001 Rmu_sc0001182.1_g000006 Rmu_ssc0000089.1_g000039
rosa_roxburghii	Rroxscaffold_1G00027090 Rroxscaffold_2G00144940
rosa_rugosa	Rorug02G0064600 Rorug05G0279000
rosa_samantha	Rh2AG111700 Rh2BG113700 Rh2CG116000 Rh2DG115000 Rh5AG350100 Rh5BG361900 Rh5CG386800 Rh5DG376800
rosa_wichuraiana	Rw2G008690 Rw5G033130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	431
AccB1I	GGYRCC	1 cut(s)	3839
AccB7I	CCANNNNNTGG	3 cut(s)	611, 2701, 3483
AccBSI	CCGCTC	1 cut(s)	744
AccI	GTMKAC	4 cut(s)	2565, 2747, 2829, 3417
AccII	CGCG	1 cut(s)	652
AciI	CCGC	4 cut(s)	9, 12, 744, 3720
AclWI	GGATC	6 cut(s)	13, 1699, 2102, 2197, 2960, 4214
AcoI	YGGCCR	3 cut(s)	127, 3542, 3746
AcsI	RAATTY	8 cut(s)	148, 752, 782, 1130, 1773, 1903, 2542, 4272
AcuI	CTGAAG	4 cut(s)	951, 1047, 1951, 3369
AfaI	GTAC	8 cut(s)	309, 391, 463, 671, 1283, 1520, 2132, 3829
AflII	CTTAAG	1 cut(s)	2984
AflIII	ACRYGT	2 cut(s)	1258, 2133
AgeI	ACCGGT	3 cut(s)	1004, 2995, 3836
AhdI	GACNNNNNGTC	1 cut(s)	3606
AjiI	CACGTC	1 cut(s)	1122
AjnI	CCWGG	8 cut(s)	1514, 1819, 2751, 3406, 3444, 3558, 4051, 4263
AjuI	GAANNNNNNNTTGG	6 cut(s)	46, 78, 508, 540, 2246, 2278
AleI	CACNNNNGTG	2 cut(s)	41, 1259
AloI	GAACNNNNNNTCC	4 cut(s)	3066, 3098, 4356, 4388
Alw21I	GWGCWC	2 cut(s)	952, 4297
Alw26I	GTCTC	9 cut(s)	186, 1202, 1291, 1938, 2135, 2189, 2985, 3861, 3946
AlwI	GGATC	6 cut(s)	13, 1699, 2102, 2197, 2960, 4214
AlwNI	CAGNNNCTG	1 cut(s)	1714
Ama87I	CYCGRG	1 cut(s)	1294
ApeKI	GCWGC	8 cut(s)	203, 206, 871, 1060, 1540, 1971, 3716, 3815
ApoI	RAATTY	8 cut(s)	148, 752, 782, 1130, 1773, 1903, 2542, 4272
ArsI	GACNNNNNNTTYG	4 cut(s)	771, 803, 1087, 1119
AseI	ATTAAT	3 cut(s)	1146, 3276, 3426
AsiGI	ACCGGT	3 cut(s)	1004, 2995, 3836
Asp700I	GAANNNNTTC	4 cut(s)	2162, 3081, 3467, 3732
AspLEI	GCGC	1 cut(s)	652
AspS9I	GGNCC	5 cut(s)	328, 1867, 3434, 4069, 4168
AsuC2I	CCSGG	2 cut(s)	1337, 3799
AsuII	TTCGAA	4 cut(s)	1501, 2764, 3823, 3998
AvaI	CYCGRG	1 cut(s)	1294
AvaII	GGWCC	3 cut(s)	328, 1867, 4069
AxyI	CCTNAGG	1 cut(s)	4077
BaeGI	GKGCMC	1 cut(s)	3844
BaeI	ACNNNNGTAYC	2 cut(s)	4112, 4145
BalI	TGGCCA	1 cut(s)	3748
BamHI	GGATCC	1 cut(s)	5
BanI	GGYRCC	1 cut(s)	3839
BanII	GRGCYC	1 cut(s)	554
BauI	CACGAG	1 cut(s)	3943
BbsI	GAAGAC	3 cut(s)	60, 361, 4431
Bbv12I	GWGCWC	2 cut(s)	952, 4297
BbvCI	CCTCAGC	1 cut(s)	4224
BbvI	GCAGC	8 cut(s)	190, 193, 858, 1047, 1552, 1983, 3728, 3802
BccI	CCATC	8 cut(s)	380, 403, 1379, 1543, 2144, 3261, 3628, 4220
BceAI	ACGGC	5 cut(s)	114, 1649, 2027, 3287, 4348
BcgI	CGANNNNNNTGC	2 cut(s)	3869, 3903
BciT130I	CCWGG	8 cut(s)	1516, 1821, 2753, 3408, 3446, 3560, 4053, 4265
BciVI	GTATCC	1 cut(s)	2306
BclI	TGATCA	2 cut(s)	853, 3988
BcnI	CCSGG	2 cut(s)	1337, 3799
BcoDI	GTCTC	9 cut(s)	186, 1202, 1291, 1938, 2135, 2189, 2985, 3861, 3946
BfaI	CTAG	3 cut(s)	1053, 3045, 3128
BfmI	CTRYAG	4 cut(s)	700, 1938, 2833, 3888
BfrI	CTTAAG	1 cut(s)	2984
BfuI	GTATCC	1 cut(s)	2306
BglI	GCCNNNNNGGC	1 cut(s)	2314
BglII	AGATCT	2 cut(s)	531, 2061
BmcAI	AGTACT	2 cut(s)	463, 3829
Bme18I	GGWCC	3 cut(s)	328, 1867, 4069
BmeRI	GACNNNNNGTC	1 cut(s)	3606
BmeT110I	CYCGRG	1 cut(s)	1294
BmgBI	CACGTC	1 cut(s)	1122
BmgT120I	GGNCC	5 cut(s)	328, 1867, 3434, 4069, 4168
BmiI	GGNNCC	5 cut(s)	7, 330, 1998, 3841, 3950
BmrI	ACTGGG	3 cut(s)	172, 2646, 4198
BmuI	ACTGGG	3 cut(s)	172, 2646, 4198
BpiI	GAAGAC	3 cut(s)	60, 361, 4431
BpmI	CTGGAG	4 cut(s)	744, 1788, 3581, 3690
Bpu10I	CCTNAGC	1 cut(s)	4224
Bpu14I	TTCGAA	4 cut(s)	1501, 2764, 3823, 3998
BpuEI	CTTGAG	5 cut(s)	425, 1595, 3650, 4287, 4394
BpuMI	CCSGG	2 cut(s)	1337, 3799
Bsa29I	ATCGAT	1 cut(s)	519
BsaI	GGTCTC	2 cut(s)	1938, 2985
BsaWI	WCCGGW	3 cut(s)	1004, 2995, 3836
BsaXI	ACNNNNNCTCC	2 cut(s)	3066, 3096
Bse118I	RCCGGY	5 cut(s)	1004, 2418, 2995, 3226, 3836
Bse21I	CCTNAGG	1 cut(s)	4077
Bse3DI	GCAATG	4 cut(s)	1746, 2934, 3292, 3904
BseBI	CCWGG	8 cut(s)	1516, 1821, 2753, 3408, 3446, 3560, 4053, 4265
BseCI	ATCGAT	1 cut(s)	519
BseGI	GGATG	9 cut(s)	1010, 1219, 1428, 2275, 3158, 3253, 3334, 4023, 4255
BseMI	GCAATG	4 cut(s)	1746, 2934, 3292, 3904
BseRI	GAGGAG	3 cut(s)	1859, 2009, 2911
BseSI	GKGCMC	1 cut(s)	3844
BseXI	GCAGC	8 cut(s)	190, 193, 858, 1047, 1552, 1983, 3728, 3802
BseYI	CCCAGC	1 cut(s)	2657
BsgI	GTGCAG	1 cut(s)	4077
Bsh1236I	CGCG	1 cut(s)	652
BshNI	GGYRCC	1 cut(s)	3839
BshTI	ACCGGT	3 cut(s)	1004, 2995, 3836
BshVI	ATCGAT	1 cut(s)	519
BsiHKAI	GWGCWC	2 cut(s)	952, 4297
BsiHKCI	CYCGRG	1 cut(s)	1294
BsiSI	CCGG	8 cut(s)	1005, 1337, 2419, 2996, 3227, 3541, 3798, 3837
BslFI	GGGAC	2 cut(s)	1796, 2653
BsmAI	GTCTC	9 cut(s)	186, 1202, 1291, 1938, 2135, 2189, 2985, 3861, 3946
BsmBI	CGTCTC	2 cut(s)	1202, 2135
BsmFI	GGGAC	2 cut(s)	1796, 2653
BsmI	GAATGC	3 cut(s)	1079, 2341, 3515
Bso31I	GGTCTC	2 cut(s)	1938, 2985
BsoBI	CYCGRG	1 cut(s)	1294
Bsp119I	TTCGAA	4 cut(s)	1501, 2764, 3823, 3998
Bsp1286I	GDGCHC	4 cut(s)	554, 952, 3844, 4297
Bsp1407I	TGTACA	1 cut(s)	1281
Bsp19I	CCATGG	5 cut(s)	1684, 2167, 2309, 2523, 4124
BspACI	CCGC	4 cut(s)	9, 12, 744, 3720
BspDI	ATCGAT	1 cut(s)	519
BspFNI	CGCG	1 cut(s)	652
BspHI	TCATGA	1 cut(s)	2590
BspLI	GGNNCC	5 cut(s)	7, 330, 1998, 3841, 3950
BspMAI	CTGCAG	2 cut(s)	1942, 3892
BspPI	GGATC	6 cut(s)	13, 1699, 2102, 2197, 2960, 4214
BspQI	GCTCTTC	2 cut(s)	162, 3216
BspT104I	TTCGAA	4 cut(s)	1501, 2764, 3823, 3998
BspT107I	GGYRCC	1 cut(s)	3839
BspTI	CTTAAG	1 cut(s)	2984
BspTNI	GGTCTC	2 cut(s)	1938, 2985
BsrBI	CCGCTC	1 cut(s)	744
BsrDI	GCAATG	4 cut(s)	1746, 2934, 3292, 3904
BsrFI	RCCGGY	5 cut(s)	1004, 2418, 2995, 3226, 3836
BsrGI	TGTACA	1 cut(s)	1281
BssAI	RCCGGY	5 cut(s)	1004, 2418, 2995, 3226, 3836
BssNAI	GTATAC	1 cut(s)	2566
BssSI	CACGAG	1 cut(s)	3943
BssT1I	CCWWGG	6 cut(s)	1684, 2167, 2309, 2523, 3264, 4124
Bst1107I	GTATAC	1 cut(s)	2566
Bst2BI	CACGAG	1 cut(s)	3943
Bst2UI	CCWGG	8 cut(s)	1516, 1821, 2753, 3408, 3446, 3560, 4053, 4265
BstAFI	CTTAAG	1 cut(s)	2984
BstAPI	GCANNNNNTGC	2 cut(s)	2392, 3839
BstAUI	TGTACA	1 cut(s)	1281
BstBI	TTCGAA	4 cut(s)	1501, 2764, 3823, 3998
BstDSI	CCRYGG	5 cut(s)	1684, 2167, 2309, 2523, 4124
BstENI	CCTNNNNNAGG	4 cut(s)	2433, 2973, 3891, 4076
BstF5I	GGATG	9 cut(s)	1010, 1219, 1428, 2275, 3158, 3253, 3334, 4023, 4255
BstFNI	CGCG	1 cut(s)	652
BstHHI	GCGC	1 cut(s)	652
BstMAI	GTCTC	9 cut(s)	186, 1202, 1291, 1938, 2135, 2189, 2985, 3861, 3946
BstNI	CCWGG	8 cut(s)	1516, 1821, 2753, 3408, 3446, 3560, 4053, 4265
BstNSI	RCATGY	7 cut(s)	176, 213, 1262, 1965, 2042, 2137, 3764
BstSFI	CTRYAG	4 cut(s)	700, 1938, 2833, 3888
BstSLI	GKGCMC	1 cut(s)	3844
BstUI	CGCG	1 cut(s)	652
BstV1I	GCAGC	8 cut(s)	190, 193, 858, 1047, 1552, 1983, 3728, 3802
BstV2I	GAAGAC	3 cut(s)	60, 361, 4431
BstX2I	RGATCY	6 cut(s)	5, 531, 2061, 2107, 2189, 4219
BstXI	CCANNNNNNTGG	1 cut(s)	564
BstYI	RGATCY	6 cut(s)	5, 531, 2061, 2107, 2189, 4219
BstZ17I	GTATAC	1 cut(s)	2566
Bsu15I	ATCGAT	1 cut(s)	519
Bsu36I	CCTNAGG	1 cut(s)	4077
BsuI	GTATCC	1 cut(s)	2306
BsuTUI	ATCGAT	1 cut(s)	519
BtgI	CCRYGG	5 cut(s)	1684, 2167, 2309, 2523, 4124
BtgZI	GCGATG	3 cut(s)	177, 247, 1521
BtrI	CACGTC	1 cut(s)	1122
BtsCI	GGATG	9 cut(s)	1010, 1219, 1428, 2275, 3158, 3253, 3334, 4023, 4255
BtsI	GCAGTG	5 cut(s)	772, 882, 1981, 2211, 4425
CaiI	CAGNNNCTG	1 cut(s)	1714
CciI	TCATGA	1 cut(s)	2590
CfoI	GCGC	1 cut(s)	652
Cfr10I	RCCGGY	5 cut(s)	1004, 2418, 2995, 3226, 3836
Cfr13I	GGNCC	5 cut(s)	328, 1867, 3434, 4069, 4168
ClaI	ATCGAT	1 cut(s)	519
Csp6I	GTAC	8 cut(s)	308, 390, 462, 670, 1282, 1519, 2131, 3828
CspAI	ACCGGT	3 cut(s)	1004, 2995, 3836
CviQI	GTAC	8 cut(s)	308, 390, 462, 670, 1282, 1519, 2131, 3828
DraI	TTTAAA	1 cut(s)	2536
DriI	GACNNNNNGTC	1 cut(s)	3606
EaeI	YGGCCR	3 cut(s)	127, 3542, 3746
Eam1105I	GACNNNNNGTC	1 cut(s)	3606
Eco130I	CCWWGG	6 cut(s)	1684, 2167, 2309, 2523, 3264, 4124
Eco24I	GRGCYC	1 cut(s)	554
Eco31I	GGTCTC	2 cut(s)	1938, 2985
Eco32I	GATATC	1 cut(s)	973
Eco47I	GGWCC	3 cut(s)	328, 1867, 4069
Eco57I	CTGAAG	4 cut(s)	951, 1047, 1951, 3369
Eco81I	CCTNAGG	1 cut(s)	4077
Eco88I	CYCGRG	1 cut(s)	1294
EcoNI	CCTNNNNNAGG	4 cut(s)	2433, 2973, 3891, 4076
EcoO109I	RGGNCCY	4 cut(s)	328, 3434, 4069, 4168
EcoRI	GAATTC	3 cut(s)	1903, 2542, 4272
EcoRII	CCWGG	8 cut(s)	1514, 1819, 2751, 3406, 3444, 3558, 4051, 4263
EcoRV	GATATC	1 cut(s)	973
EcoT14I	CCWWGG	6 cut(s)	1684, 2167, 2309, 2523, 3264, 4124
EcoT22I	ATGCAT	1 cut(s)	3762
EcoT38I	GRGCYC	1 cut(s)	554
ErhI	CCWWGG	6 cut(s)	1684, 2167, 2309, 2523, 3264, 4124
Esp3I	CGTCTC	2 cut(s)	1202, 2135
FalI	AAGNNNNNCTT	8 cut(s)	285, 317, 702, 734, 2967, 2999, 3720, 3752
FaqI	GGGAC	2 cut(s)	1796, 2653
FbaI	TGATCA	2 cut(s)	853, 3988
FblI	GTMKAC	4 cut(s)	2565, 2747, 2829, 3417
FokI	GGATG	9 cut(s)	997, 1226, 1415, 2282, 3165, 3240, 3341, 4010, 4242
FriOI	GRGCYC	1 cut(s)	554
FspBI	CTAG	3 cut(s)	1053, 3045, 3128
GlaI	GCGC	1 cut(s)	651
GsaI	CCCAGC	1 cut(s)	2661
GsuI	CTGGAG	4 cut(s)	744, 1788, 3581, 3690
HapII	CCGG	8 cut(s)	1005, 1337, 2419, 2996, 3227, 3541, 3798, 3837
HhaI	GCGC	1 cut(s)	652
Hin6I	GCGC	1 cut(s)	650
HinP1I	GCGC	1 cut(s)	650
HincII	GTYRAC	1 cut(s)	1231
HindII	GTYRAC	1 cut(s)	1231
HindIII	AAGCTT	3 cut(s)	102, 1760, 2679
HpaII	CCGG	8 cut(s)	1005, 1337, 2419, 2996, 3227, 3541, 3798, 3837
Hpy166II	GTNNAC	9 cut(s)	670, 1231, 2254, 2566, 2748, 2830, 3001, 3418, 4114
Hpy8I	GTNNAC	9 cut(s)	670, 1231, 2254, 2566, 2748, 2830, 3001, 3418, 4114
Hpy99I	CGWCG	1 cut(s)	1126
HpyCH4IV	ACGT	3 cut(s)	1102, 1121, 2144
HpySE526I	ACGT	3 cut(s)	1102, 1121, 2144
HspAI	GCGC	1 cut(s)	650
KroI	GCCGGC	1 cut(s)	3226
KroNI	GCCGGC	1 cut(s)	3228
Ksp22I	TGATCA	2 cut(s)	853, 3988
LguI	GCTCTTC	2 cut(s)	162, 3216
LmnI	GCTCC	9 cut(s)	955, 1312, 1475, 1823, 1996, 2573, 2926, 3671, 3902
Lsp1109I	GCAGC	8 cut(s)	190, 193, 858, 1047, 1552, 1983, 3728, 3802
MaeI	CTAG	3 cut(s)	1053, 3045, 3128
MaeII	ACGT	3 cut(s)	1102, 1121, 2144
MaeIII	GTNAC	8 cut(s)	43, 539, 1348, 1691, 2991, 3196, 3369, 4087
MbiI	CCGCTC	1 cut(s)	744
MfeI	CAATTG	2 cut(s)	609, 3873
MflI	RGATCY	6 cut(s)	5, 531, 2061, 2107, 2189, 4219
MhlI	GDGCHC	4 cut(s)	554, 952, 3844, 4297
MlsI	TGGCCA	1 cut(s)	3748
MluNI	TGGCCA	1 cut(s)	3748
MlyI	GAGTC	6 cut(s)	260, 355, 908, 1286, 3269, 4375
MmeI	TCCRAC	5 cut(s)	961, 1290, 3268, 4164, 4403
Mox20I	TGGCCA	1 cut(s)	3748
Mph1103I	ATGCAT	1 cut(s)	3762
MroNI	GCCGGC	1 cut(s)	3226
MroXI	GAANNNNTTC	4 cut(s)	2162, 3081, 3467, 3732
MscI	TGGCCA	1 cut(s)	3748
MslI	CAYNNNNRTG	6 cut(s)	41, 665, 1259, 2595, 2961, 3008
Msp20I	TGGCCA	1 cut(s)	3748
MspA1I	CMGCKG	1 cut(s)	871
MspCI	CTTAAG	1 cut(s)	2984
MspI	CCGG	8 cut(s)	1005, 1337, 2419, 2996, 3227, 3541, 3798, 3837
MunI	CAATTG	2 cut(s)	609, 3873
Mva1269I	GAATGC	3 cut(s)	1079, 2341, 3515
MvaI	CCWGG	8 cut(s)	1516, 1821, 2753, 3408, 3446, 3560, 4053, 4265
MvnI	CGCG	1 cut(s)	652
NaeI	GCCGGC	1 cut(s)	3228
NciI	CCSGG	2 cut(s)	1337, 3799
NcoI	CCATGG	5 cut(s)	1684, 2167, 2309, 2523, 4124
NgoMIV	GCCGGC	1 cut(s)	3226
NlaIV	GGNNCC	5 cut(s)	7, 330, 1998, 3841, 3950
NmuCI	GTSAC	3 cut(s)	43, 3369, 4087
NsiI	ATGCAT	1 cut(s)	3762
NspI	RCATGY	7 cut(s)	176, 213, 1262, 1965, 2042, 2137, 3764
NspV	TTCGAA	4 cut(s)	1501, 2764, 3823, 3998
OliI	CACNNNNGTG	2 cut(s)	41, 1259
PaeI	GCATGC	3 cut(s)	176, 1965, 2042
PaeR7I	CTCGAG	1 cut(s)	1294
PagI	TCATGA	1 cut(s)	2590
PciI	ACATGT	2 cut(s)	1258, 2133
PciSI	GCTCTTC	2 cut(s)	162, 3216
PctI	GAATGC	3 cut(s)	1079, 2341, 3515
PdiI	GCCGGC	1 cut(s)	3228
PdmI	GAANNNNTTC	4 cut(s)	2162, 3081, 3467, 3732
PfeI	GAWTC	8 cut(s)	516, 1811, 2665, 3041, 3110, 3468, 3960, 4039
PflFI	GACNNNGTC	1 cut(s)	3938
PflMI	CCANNNNNTGG	3 cut(s)	611, 2701, 3483
PfoI	TCCNGGA	2 cut(s)	3558, 3797
PinAI	ACCGGT	3 cut(s)	1004, 2995, 3836
PleI	GAGTC	6 cut(s)	259, 355, 907, 1286, 3268, 4375
PpsI	GAGTC	6 cut(s)	259, 355, 907, 1286, 3268, 4375
PpuMI	RGGWCCY	2 cut(s)	328, 4069
PscI	ACATGT	2 cut(s)	1258, 2133
PshBI	ATTAAT	3 cut(s)	1146, 3276, 3426
PsiI	TTATAA	1 cut(s)	431
Psp5II	RGGWCCY	2 cut(s)	328, 4069
Psp6I	CCWGG	8 cut(s)	1514, 1819, 2751, 3406, 3444, 3558, 4051, 4263
PspFI	CCCAGC	1 cut(s)	2657
PspGI	CCWGG	8 cut(s)	1514, 1819, 2751, 3406, 3444, 3558, 4051, 4263
PspN4I	GGNNCC	5 cut(s)	7, 330, 1998, 3841, 3950
PspPI	GGNCC	5 cut(s)	328, 1867, 3434, 4069, 4168
PspPPI	RGGWCCY	2 cut(s)	328, 4069
PstI	CTGCAG	2 cut(s)	1942, 3892
PstNI	CAGNNNCTG	1 cut(s)	1714
PsuI	RGATCY	6 cut(s)	5, 531, 2061, 2107, 2189, 4219
PsyI	GACNNNGTC	1 cut(s)	3938
PvuII	CAGCTG	1 cut(s)	871
RsaI	GTAC	8 cut(s)	309, 391, 463, 671, 1283, 1520, 2132, 3829
RsaNI	GTAC	8 cut(s)	308, 390, 462, 670, 1282, 1519, 2131, 3828
RseI	CAYNNNNRTG	6 cut(s)	41, 665, 1259, 2595, 2961, 3008
SapI	GCTCTTC	2 cut(s)	162, 3216
Sau96I	GGNCC	5 cut(s)	328, 1867, 3434, 4069, 4168
ScaI	AGTACT	2 cut(s)	463, 3829
SchI	GAGTC	6 cut(s)	260, 355, 908, 1286, 3269, 4375
SduI	GDGCHC	4 cut(s)	554, 952, 3844, 4297
SfcI	CTRYAG	4 cut(s)	700, 1938, 2833, 3888
Sfr274I	CTCGAG	1 cut(s)	1294
SfuI	TTCGAA	4 cut(s)	1501, 2764, 3823, 3998
SinI	GGWCC	3 cut(s)	328, 1867, 4069
SlaI	CTCGAG	1 cut(s)	1294
SmiMI	CAYNNNNRTG	6 cut(s)	41, 665, 1259, 2595, 2961, 3008
SmlI	CTYRAG	7 cut(s)	404, 1294, 1610, 2984, 3629, 4302, 4409
SmoI	CTYRAG	7 cut(s)	404, 1294, 1610, 2984, 3629, 4302, 4409
SphI	GCATGC	3 cut(s)	176, 1965, 2042
SsiI	CCGC	4 cut(s)	9, 12, 744, 3720
SspI	AATATT	2 cut(s)	1187, 2405
SspMI	CTAG	3 cut(s)	1053, 3045, 3128
StyI	CCWWGG	6 cut(s)	1684, 2167, 2309, 2523, 3264, 4124
TaiI	ACGT	3 cut(s)	1105, 1124, 2147
TaqII	GACCGA	1 cut(s)	2459
TatI	WGTACW	4 cut(s)	461, 1281, 2130, 3827
TauI	GCSGC	2 cut(s)	14, 3722
TfiI	GAWTC	8 cut(s)	516, 1811, 2665, 3041, 3110, 3468, 3960, 4039
TseFI	GTSAC	3 cut(s)	43, 3369, 4087
TseI	GCWGC	8 cut(s)	203, 206, 871, 1060, 1540, 1971, 3716, 3815
Tsp45I	GTSAC	3 cut(s)	43, 3369, 4087
TspGWI	ACGGA	4 cut(s)	1226, 1427, 3596, 3839
Tth111I	GACNNNGTC	1 cut(s)	3938
Van91I	CCANNNNNTGG	3 cut(s)	611, 2701, 3483
Vha464I	CTTAAG	1 cut(s)	2984
VpaK11BI	GGWCC	3 cut(s)	328, 1867, 4069
VspI	ATTAAT	3 cut(s)	1146, 3276, 3426
XagI	CCTNNNNNAGG	4 cut(s)	2433, 2973, 3891, 4076
XapI	RAATTY	8 cut(s)	148, 752, 782, 1130, 1773, 1903, 2542, 4272
XbaI	TCTAGA	1 cut(s)	3044
XceI	RCATGY	7 cut(s)	176, 213, 1262, 1965, 2042, 2137, 3764
XcmI	CCANNNNNNNNNTGG	3 cut(s)	3452, 3916, 4059
XhoI	CTCGAG	1 cut(s)	1294
XmiI	GTMKAC	4 cut(s)	2565, 2747, 2829, 3417
XmnI	GAANNNNTTC	4 cut(s)	2162, 3081, 3467, 3732
XspI	CTAG	3 cut(s)	1053, 3045, 3128
ZrmI	AGTACT	2 cut(s)	463, 3829
Zsp2I	ATGCAT	1 cut(s)	3762

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.