Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0002932

Physical Location & Seq

Genomic Coordinates Forward (+)

34101388 .. 34107772

Gene Structure 6385 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00027090.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4692 bp

ATGGGATCCGCCGCAGGATTAGAACCCGACCCTCCCACCAACGAGACTAAACCGACATCTTCTTCGTCTTTCTCTGGAATGACAAAGAAGAAGGGTAAAAAAGCTTCCCAAAAGGAAGCACCTACTGGCCGTAGAAGTAGGAAGCGAAATCTGCCTCAGAGTGGTGAAGAGCCTGCCCAGTCTCGCAAAATGCCCAAGCGTGCTGCTGCATGTATAGACTTTAAGGAGAAGTCTGTCCATATTACTGATGAGTCTGATCTCATCGCAAGCAAGGAGGAACAGATTGTGCCAGAAGAAGGTCTTGCTGTACGCTTGACTTGTGGCAAGGACCCAGATAATGAGAAGCCAAGCAGAAGACTGACTCAATTTATCTTGCATAGTGAAGATGGTACTGCAAGACCACTTGAGATGGTGGAAGCGGGCCTGTTTATTACTGGCACTATATTGCCTCTTCGTGAAAGTACTGAGAAGGACAAGGACAGCAGTATTAAATGTGAAGGTTTTGGGCGAATAGATGCTTGGAAGATCTCTGGTTACGATGAGGGCTCTCCAATAATATGGCTTTCAACTAAAGTTGCCGATTATTGTTGTGTTAAATCAGCAATTGGCTACAAGAAGCACTATGATCTCTTCTTTGAGAAAGCGCGTGCTTGCATAGAGGTGTACCAGAAACTTTCAAAATGTAAATCTGGCTGTAGTCTTGAAGAATTACTTGCTGGAGTTGCTCGATCATTGAGCGGAAGCAAATTCTTTTCTGGCAGTGCATCTATCAAAGAATTTGCCCTATCTCACGGTGAGTTTATCTATAAACGATTGATAGGTTTGGAACAGTCATTGAAGAAGAATGATCAGATATTGGCACAGCTGCCTGTTCTCACTGCCCTCAGAGATGAGTCTATAAACCATGGATATTATGAGCAATCAGAACCAGTAGCTTCAAGTGCTACCTTAAAGATTGCTTCAGATATCAGAGTTGGAGGGAATGAACTGAGAACAACCGGTTCATCCATAGTTGAAGCTGAGGAAGATGAAGACACAAAGTTAGCTAGATTGCTGCAAGAAGAAGAATGCTTTAAGTCTATGAAACAAAGAAAACGTCAGGGTGCTACCTCCACGTCGAGTAAATTCTACATCAAAATTAATGAAGATGAAATTGCTAATGATTATCCTGAACCTAAATATTATGACAATTCCATTGAAGAGACGGATGAGTTTATTGTTGTTGACAGTGAGGCTGGTGATCTTCCCACACATGTGCTTGATAATTGGTGTCTGTACAACTTAGACTCGAGACTGATTTCGTTGGAGCTTCTTCCAATGACAGATTGCCCAGATATTGATGTTACAATTTTTGGGTCAGGGGTTATGAGTGAAGATGATGGAAGCTGTGGCTTTAATCTCGATGCTGACAGTATCCAGTCTTCTTCAAGTGGTTCAGGGGCACCAGTAGCAGAAGATGCTTATGGCATCCCAATTTATTTGAGTGCCATAAAGGAATGGGTGATAGAATGGGGAGCATCAATGATCTCAGTATCAATTCGAACAGAGCTGGCCTGGTACAGACTTGGCAAGCCATCACAGCAGTATGCTTTGTGGTATGAACCAATACTGAAAACAGCAAGGGTTGGGAGAAGTATAATCACTATGCTCAAGGCTGAACGTCGAGTAGCACGGCTTTCTTTTGCAGATGTCATTAAACGATTGTCAGAGTTCCCAAAGAACCATGGTGGTTACATTTCTTCTGATCCAGCATCTGTTGAGAGATATGTGGTTGTACATGGAGCGATAATATTGCAGCTGTTTTCACAGTTTCCAGATGTGAAGATTAAAAAATGTTCATTTCTTGATGGTCTTCGAGACAAAATGGAGAAGAGGCACCATACTAAATGGGAAGTAAAGAGCAAGAAAATTTTGCAGAAGAGTGAATCAAATTTGAACCCACGGGCATCAATGGGACCTGTGGTTTCCAAGAGGAAGGCAATGCAGGCTACAACAACAAAGCTTATCAACAGAATTTGGGGGGACTACTATTCAAATTACTCTCCAGTAGATTCAAGCCCAGGAGCTACTTGTGGAAAGGTAGAGGAGAATGAAGTTATAGAAGAGGTCCAAGAGGACATAGAAGAGTATGATGTAGATGAGAATTCATCAGTAATGGAACAACCCCAAAGCTCTTCTGCAGTTTCAAGAAAGCCTAAAGCATGCTTCAGCAGCAGTGAGATTTTGTGGGGTGACGAGCCTGTGGGAAAAACCTGTTCTGGTGAAGCTCTCTACAAGCATGCCGTTCTTTGTGGAGATGAGATCTCTGTGGGTGGTGCTGTTTTTGTGGATATTGATGTATCAAAAGATCCTGTTATATATCTAGTTGAGTACATGTTTGAGACGTTAGATGGAAAGAAGATGTTCCATGGGAGAATGATGCAGAGAGGATCTGAGACTGTTCTTGGAAACACTGCTAATGAGAGAGAAGTGTTTTTAACTGATGAGTGCGTGAACTTGGCATTGGAGGATGTGGAACAGACTGTTGTTGTGGGTATCCAATCAATGCCATGGGGGCATCAGTATAGGAAGAAGAATGCCGATACTAATAGAATTGATAGAGCAAGAGCAGAAGAGAGGAAGAAGAAAGGATTGCCAACAGAATATTACTGTAAAAGCCGGTATTGCCCTGAGAAAGGTGCTTTCTTTAGTCTTTCTCATCATACTATCGGTCTGGGTTCTGGTTTTTGCCACTCTTGCAAAGTTAATGAAGATGAAAAGGCCAAGGAAGTCTTTAAAGTGAATTCATCAAAGACTGGTTTTGTACACAGTGGAGCTGAGTATTTCATTCATGATTATGTCTATGTAAGCCCCTATCATTTTTCTGTGGAAAGGAATGGGACTGAGCTTTTCAAGGCTGGGAGGAATCTGGGATTGAAAGCTTATGTTGTGTGCCAAGTGCTGGAAATAATTACTGCAAAGGAATATAAACAACCTGATATGAAGTCTACCCAGGTTAAAGTTCGAAGGTTTTTCAGACCAGAGGACATTTCAGTAGAGAAGGCATACTGCTCTGATGTCAGAGAGGTCTACTACAGTGAAGAAACACACATTATCCCTGCTGATAGCTTAGAAGGAAAATGTGAAGTTAGAAAGAGGAGTGATCTTCCAGAATGTGATGCTCCTGCAATGTTTGAGCATATTTTCTTCTGTGAGCATTTATATGATCCTTCTAAAGGGTCTCTTAAGCAGTTACCAGTTCACATCAAGGTGAGGCACTCAGCAGTTGGTGGTGATGCTGAATCTAGAAAGAAAAAGGGCAAGTGCAAAGAAGGAGAAGATGTTCCAGAAGTTGAGAAACAGAGAGATGATTCGGAACACAAACGCCTAGCCACTTTGGATATATTTGCTGGATGTGGTGGCCTGTCAGAAGGTTTGCATCAGGCTGGTGTTTCAGTAACGAAGTGGGCAATCGAGTATGAAGAGCCGGCTGGACAGGCTTTCCAACTCAACCATCCTGAGTCCAAGGTGTTCATTAATAATTGCAATGTCATCTTGAAAGCCGTCATGGACAAATGTGGAGATATGGATGATTGCATCTCCACTACTGAAGCCAATGATTTAGCCTCATCACTTGATGAGAAGGAGAAAGACAATTTGCCCTTGCCAGGGCAAGTGGACTTTATTAATGGAGGCCCTCCATGCCAGGGTTTTTCTGGTATGAATAGATTCAATACCAGCACTTGGAGTAAAGTTCAGTGTGAAATGATACTGGCATTCTTATCCTTTGCTGATTACTTCCGCCCAAAATACTTCCTCCTGGAGAATGTGAGAAACTTTGTGGCTTTCAATAAAGGACAGACATTCCGTCTGACAGTGGCTTCACTTCTTGAGATGGGTTATCAGGTGAGGTTTGGTGTTCTAGAAGCTGGAGCTTATGGGGTTTCTCAATCCCGAAAGCGTGCATTTATCTGGGCAGCCGCACCAGAAGAGGTTCTTCCAGATTGGCCAGAGCCAATGCATGTTTTTGGTGTGCCAGAGTTGAAGATCAATTTATCCGGGAACTCCTATTATGCTGCTGTTCGAAGTACTGCAACGGGTGCCCCATTCCGTGCCATCACAGTAAGAGACACAATTGGCGATCTTCCAGCTGTAGGAAATGGAGCTTCCAAGATCAATTTGGAATATGAAACCGACCCTGTCTCGTGGTTCCAGAAGAGAATCAGAGGGAACATGGCTGTTTTGACTGATCACATTTCGAAAGAAATGAACGAGCTGAACCTCATCCGATGCAAGAAGATTCCCAAGAGGCCAGGTGCAGATTGGCAGGACCTTCCTGAGGAAAAGGTGACGCTCTCTACTGGTCAAATAGTGGACTTGATACCATGGTGCTTGCCCAACACAGCGAAACGCCACAATCAGTGGAAGGGCCTGTTTGGAAGGTTGGACTGGGAAGGCAACTTCCCAACCTCCATCACAGATCCTCAGCCAATGGGTAAGGTGGGAATGTGCTTTCATCCTGACCAGGACAGAATTCTGACAGTTAGGGAATGTGCTCGATCTCAAGGCTTTCCTGACAGCTACCAGTTTTATGGCAACATTCTTCACAAGCATAGGCAGATTGGAAATGCTGTTCCTCCGACTCTGGCTTATGCTCTGGGCAGAAAACTCAAGGAAGCAGTGGATAGCAAGAAGTCTTCTTCACAAGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000278 GO:0000281 GO:0000910 GO:0003006 GO:0003674 GO:0003824 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006349 GO:0006725 GO:0006807 GO:0007049 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008356 GO:0009292 GO:0009294 GO:0009790 GO:0009791 GO:0009793 GO:0009909 GO:0009910 GO:0009987 GO:0010069 GO:0010070 GO:0010154 GO:0010216 GO:0010424 GO:0010468 GO:0016740 GO:0016741 GO:0019222 GO:0022402 GO:0022414 GO:0032259 GO:0032501 GO:0032502 GO:0032776 GO:0034641 GO:0040029 GO:0043170 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044728 GO:0044764 GO:0046483 GO:0048316 GO:0048519 GO:0048580 GO:0048581 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0051093 GO:0051239 GO:0051241 GO:0051301 GO:0051704 GO:0060255 GO:0061458 GO:0061640 GO:0065007 GO:0071514 GO:0071704 GO:0090304 GO:1901360 GO:1903047 GO:2000026 GO:2000241 GO:2000242

KEGG Pathways

Metabolic & Signaling

ko00270 ko01100 ko05206 map00270 map01100 map05206

Pfam Domains

Protein Families

BAH DNA_methylase DNMT1-RFD

Protein Analysis

1563

Amino Acids

174.72

Weight (kDa)

5.99

Isoelectric Point (pI)

47.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DNMT1-RFD	PF12047	113 - 243	6.3e-30	Cytosine specific DNA methyltransferase replication foci domain
DNMT1-RFD	PF12047	414 - 570	3.4e-31	Cytosine specific DNA methyltransferase replication foci domain
BAH	PF01426	767 - 898	4.9e-18	BAH domain
BAH	PF01426	941 - 1079	2.7e-25	BAH domain
DNA_methylase	PF00145	1124 - 1551	1.2e-47	C-5 cytosine-specific DNA methylase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002932)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G08990 AT4G14140 AT4G14140 AT4G14140 AT5G49160 AT5G49160
fragaria_vesca	FvH4_1g10300 FvH4_1g10300
malus_domestica	MD02G1115100.v1.1 MD15G1231100.v1.1
prunus_persica	Prupe.7G060100_v2.0.a1 Prupe.7G183100_v2.0.a1 Prupe.7G183100_v2.0.a1
pyrus_communis	pycom02g09000 pycom02g09010 pycom15g20590 pycom15g20600
rosa_chinensis	RchiOBHm_Chr2g0097141 RchiOBHm_Chr5g0051581
rosa_laevigata	RLG00000016706 RLG00000034873
rosa_multiflora	Rmu_co8502141.1_g000001 Rmu_sc0001182.1_g000006 Rmu_ssc0000089.1_g000039
rosa_roxburghii	Rroxscaffold_1G00027090 Rroxscaffold_2G00144940
rosa_rugosa	Rorug02G0064600 Rorug05G0279000
rosa_samantha	Rh2AG111700 Rh2BG113700 Rh2CG116000 Rh2DG115000 Rh5AG350100 Rh5BG361900 Rh5CG386800 Rh5DG376800
rosa_wichuraiana	Rw2G008690 Rw5G033130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	3 cut(s)	1441, 1875, 4079
AccB7I	CCANNNNNTGG	2 cut(s)	2941, 3723
AccBSI	CCGCTC	1 cut(s)	738
AccI	GTMKAC	2 cut(s)	2987, 3069
AccII	CGCG	1 cut(s)	646
AciI	CCGC	6 cut(s)	9, 12, 419, 738, 3781, 3960
AclWI	GGATC	6 cut(s)	13, 1739, 2342, 2437, 3200, 4454
AcoI	YGGCCR	2 cut(s)	127, 3986
AcsI	RAATTY	9 cut(s)	746, 776, 1124, 1908, 1930, 2013, 2143, 2782, 4512
AcuI	CTGAAG	3 cut(s)	945, 2191, 3609
AflII	CTTAAG	1 cut(s)	3224
AflIII	ACRYGT	2 cut(s)	1252, 2373
AgeI	ACCGGT	1 cut(s)	998
AhdI	GACNNNNNGTC	1 cut(s)	3846
AjiI	CACGTC	1 cut(s)	1116
AjnI	CCWGG	8 cut(s)	1553, 2059, 2991, 3646, 3684, 3798, 4291, 4503
AjuI	GAANNNNNNNTTGG	4 cut(s)	502, 534, 2486, 2518
AleI	CACNNNNGTG	1 cut(s)	1253
AloI	GAACNNNNNNTCC	4 cut(s)	3306, 3338, 4596, 4628
Alw21I	GWGCWC	1 cut(s)	4537
AlwI	GGATC	6 cut(s)	13, 1739, 2342, 2437, 3200, 4454
AlwNI	CAGNNNCTG	1 cut(s)	1754
Ama87I	CYCGRG	1 cut(s)	1288
AoxI	GGCC	9 cut(s)	127, 421, 1551, 2760, 3400, 3673, 3986, 4289, 4408
ApeKI	GCWGC	8 cut(s)	203, 206, 865, 1054, 1795, 2211, 3956, 4055
ApoI	RAATTY	9 cut(s)	746, 776, 1124, 1908, 1930, 2013, 2143, 2782, 4512
ArsI	GACNNNNNNTTYG	4 cut(s)	46, 78, 1081, 1113
AseI	ATTAAT	3 cut(s)	1140, 3516, 3666
AsiGI	ACCGGT	1 cut(s)	998
Asp700I	GAANNNNTTC	4 cut(s)	2402, 3321, 3707, 3972
AspLEI	GCGC	1 cut(s)	646
AspS9I	GGNCC	7 cut(s)	328, 421, 1955, 2107, 3674, 4309, 4408
AsuC2I	CCSGG	1 cut(s)	4039
AsuII	TTCGAA	4 cut(s)	1540, 3004, 4063, 4238
AvaI	CYCGRG	1 cut(s)	1288
AvaII	GGWCC	4 cut(s)	328, 1955, 2107, 4309
AxyI	CCTNAGG	1 cut(s)	4317
BaeGI	GKGCMC	2 cut(s)	1444, 4084
BaeI	ACNNNNGTAYC	2 cut(s)	4352, 4385
BalI	TGGCCA	1 cut(s)	3988
BamHI	GGATCC	1 cut(s)	5
BanI	GGYRCC	3 cut(s)	1441, 1875, 4079
BanII	GRGCYC	1 cut(s)	548
BarI	GAAGNNNNNNTAC	4 cut(s)	88, 120, 1405, 1437
BauI	CACGAG	1 cut(s)	4183
BbsI	GAAGAC	5 cut(s)	361, 1038, 1413, 1844, 4668
Bbv12I	GWGCWC	1 cut(s)	4537
BbvCI	CCTCAGC	2 cut(s)	1020, 4464
BbvI	GCAGC	8 cut(s)	190, 193, 852, 1041, 1807, 2223, 3968, 4042
BceAI	ACGGC	4 cut(s)	114, 1688, 2267, 3527
BcgI	CGANNNNNNTGC	2 cut(s)	1774, 1808
BciT130I	CCWGG	8 cut(s)	1555, 2061, 2993, 3648, 3686, 3800, 4293, 4505
BciVI	GTATCC	2 cut(s)	1424, 2546
BclI	TGATCA	2 cut(s)	847, 4228
BcnI	CCSGG	1 cut(s)	4039
BfaI	CTAG	5 cut(s)	1047, 2363, 3285, 3368, 3902
BfmI	CTRYAG	4 cut(s)	694, 2178, 3073, 4131
BfrI	CTTAAG	1 cut(s)	3224
BfuI	GTATCC	2 cut(s)	1424, 2546
BglI	GCCNNNNNGGC	1 cut(s)	2554
BglII	AGATCT	2 cut(s)	525, 2301
BmcAI	AGTACT	2 cut(s)	463, 4069
Bme1390I	CCNGG	9 cut(s)	1555, 2061, 2993, 3648, 3686, 3800, 4039, 4293, 4505
Bme18I	GGWCC	4 cut(s)	328, 1955, 2107, 4309
BmeRI	GACNNNNNGTC	1 cut(s)	3846
BmeT110I	CYCGRG	1 cut(s)	1288
BmgBI	CACGTC	1 cut(s)	1116
BmgT120I	GGNCC	7 cut(s)	328, 421, 1955, 2107, 3674, 4309, 4408
BmiI	GGNNCC	7 cut(s)	7, 330, 1443, 1877, 1956, 4081, 4190
BmrFI	CCNGG	9 cut(s)	1555, 2061, 2993, 3648, 3686, 3800, 4039, 4293, 4505
BmrI	ACTGGG	2 cut(s)	172, 4438
BmuI	ACTGGG	2 cut(s)	172, 4438
BpiI	GAAGAC	5 cut(s)	361, 1038, 1413, 1844, 4668
BpmI	CTGGAG	4 cut(s)	738, 2028, 3821, 3930
Bpu10I	CCTNAGC	2 cut(s)	1020, 4464
Bpu14I	TTCGAA	4 cut(s)	1540, 3004, 4063, 4238
BpuEI	CTTGAG	5 cut(s)	425, 1634, 3890, 4527, 4634
BpuMI	CCSGG	1 cut(s)	4039
BsaI	GGTCTC	1 cut(s)	3225
BsaWI	WCCGGW	1 cut(s)	998
BsaXI	ACNNNNNCTCC	2 cut(s)	3306, 3336
Bse118I	RCCGGY	3 cut(s)	998, 2658, 3466
Bse21I	CCTNAGG	1 cut(s)	4317
Bse3DI	GCAATG	3 cut(s)	1986, 3174, 3532
BseBI	CCWGG	8 cut(s)	1555, 2061, 2993, 3648, 3686, 3800, 4293, 4505
BseGI	GGATG	9 cut(s)	1004, 1213, 1467, 2515, 3398, 3493, 3574, 4263, 4495
BseMI	GCAATG	3 cut(s)	1986, 3174, 3532
BseRI	GAGGAG	2 cut(s)	2099, 3151
BseSI	GKGCMC	2 cut(s)	1444, 4084
BseXI	GCAGC	8 cut(s)	190, 193, 852, 1041, 1807, 2223, 3968, 4042
BseYI	CCCAGC	1 cut(s)	2897
BsgI	GTGCAG	1 cut(s)	4317
Bsh1236I	CGCG	1 cut(s)	646
BshFI	GGCC	9 cut(s)	129, 423, 1553, 2762, 3402, 3675, 3988, 4291, 4410
BshNI	GGYRCC	3 cut(s)	1441, 1875, 4079
BshTI	ACCGGT	1 cut(s)	998
BsiHKAI	GWGCWC	1 cut(s)	4537
BsiHKCI	CYCGRG	1 cut(s)	1288
BsiSI	CCGG	4 cut(s)	999, 2659, 3467, 4038
BslFI	GGGAC	3 cut(s)	1968, 2036, 2893
BsmBI	CGTCTC	2 cut(s)	1196, 2375
BsmFI	GGGAC	3 cut(s)	1968, 2036, 2893
BsmI	GAATGC	3 cut(s)	1073, 2581, 3755
BsnI	GGCC	9 cut(s)	129, 423, 1553, 2762, 3402, 3675, 3988, 4291, 4410
Bso31I	GGTCTC	1 cut(s)	3225
BsoBI	CYCGRG	1 cut(s)	1288
Bsp119I	TTCGAA	4 cut(s)	1540, 3004, 4063, 4238
Bsp1286I	GDGCHC	4 cut(s)	548, 1444, 4084, 4537
Bsp1407I	TGTACA	3 cut(s)	1275, 1774, 2803
Bsp19I	CCATGG	5 cut(s)	904, 1723, 2407, 2549, 4364
BspACI	CCGC	6 cut(s)	9, 12, 419, 738, 3781, 3960
BspANI	GGCC	9 cut(s)	129, 423, 1553, 2762, 3402, 3675, 3988, 4291, 4410
BspFNI	CGCG	1 cut(s)	646
BspHI	TCATGA	1 cut(s)	2830
BspLI	GGNNCC	7 cut(s)	7, 330, 1443, 1877, 1956, 4081, 4190
BspMAI	CTGCAG	1 cut(s)	2182
BspPI	GGATC	6 cut(s)	13, 1739, 2342, 2437, 3200, 4454
BspQI	GCTCTTC	3 cut(s)	162, 2179, 3456
BspT104I	TTCGAA	4 cut(s)	1540, 3004, 4063, 4238
BspT107I	GGYRCC	3 cut(s)	1441, 1875, 4079
BspTI	CTTAAG	1 cut(s)	3224
BspTNI	GGTCTC	1 cut(s)	3225
BsrBI	CCGCTC	1 cut(s)	738
BsrDI	GCAATG	3 cut(s)	1986, 3174, 3532
BsrFI	RCCGGY	3 cut(s)	998, 2658, 3466
BsrGI	TGTACA	3 cut(s)	1275, 1774, 2803
BssAI	RCCGGY	3 cut(s)	998, 2658, 3466
BssSI	CACGAG	1 cut(s)	4183
BssT1I	CCWWGG	7 cut(s)	904, 1723, 2407, 2549, 2763, 3504, 4364
Bst2BI	CACGAG	1 cut(s)	4183
Bst2UI	CCWGG	8 cut(s)	1555, 2061, 2993, 3648, 3686, 3800, 4293, 4505
BstAFI	CTTAAG	1 cut(s)	3224
BstAPI	GCANNNNNTGC	2 cut(s)	1457, 4079
BstAUI	TGTACA	3 cut(s)	1275, 1774, 2803
BstBI	TTCGAA	4 cut(s)	1540, 3004, 4063, 4238
BstDSI	CCRYGG	6 cut(s)	904, 1723, 1940, 2407, 2549, 4364
BstENI	CCTNNNNNAGG	3 cut(s)	2673, 3213, 4316
BstF5I	GGATG	9 cut(s)	1004, 1213, 1467, 2515, 3398, 3493, 3574, 4263, 4495
BstFNI	CGCG	1 cut(s)	646
BstHHI	GCGC	1 cut(s)	646
BstNI	CCWGG	8 cut(s)	1555, 2061, 2993, 3648, 3686, 3800, 4293, 4505
BstNSI	RCATGY	6 cut(s)	213, 1256, 2205, 2282, 2377, 4004
BstSCI	CCNGG	9 cut(s)	1553, 2059, 2991, 3646, 3684, 3798, 4037, 4291, 4503
BstSFI	CTRYAG	4 cut(s)	694, 2178, 3073, 4131
BstSLI	GKGCMC	2 cut(s)	1444, 4084
BstUI	CGCG	1 cut(s)	646
BstV1I	GCAGC	8 cut(s)	190, 193, 852, 1041, 1807, 2223, 3968, 4042
BstV2I	GAAGAC	5 cut(s)	361, 1038, 1413, 1844, 4668
BstX2I	RGATCY	6 cut(s)	5, 525, 2301, 2347, 2429, 4459
BstXI	CCANNNNNNTGG	2 cut(s)	558, 4571
BstYI	RGATCY	6 cut(s)	5, 525, 2301, 2347, 2429, 4459
Bsu36I	CCTNAGG	1 cut(s)	4317
BsuI	GTATCC	2 cut(s)	1424, 2546
BsuRI	GGCC	9 cut(s)	129, 423, 1553, 2762, 3402, 3675, 3988, 4291, 4410
BtgI	CCRYGG	6 cut(s)	904, 1723, 1940, 2407, 2549, 4364
BtgZI	GCGATG	1 cut(s)	247
BtrI	CACGTC	1 cut(s)	1116
BtsCI	GGATG	9 cut(s)	1004, 1213, 1467, 2515, 3398, 3493, 3574, 4263, 4495
BtsI	GCAGTG	5 cut(s)	766, 876, 2221, 2451, 4665
CaiI	CAGNNNCTG	1 cut(s)	1754
CciI	TCATGA	1 cut(s)	2830
CfoI	GCGC	1 cut(s)	646
Cfr10I	RCCGGY	3 cut(s)	998, 2658, 3466
Cfr13I	GGNCC	7 cut(s)	328, 421, 1955, 2107, 3674, 4309, 4408
CseI	GACGC	1 cut(s)	4339
CspAI	ACCGGT	1 cut(s)	998
DraI	TTTAAA	1 cut(s)	2776
DriI	GACNNNNNGTC	1 cut(s)	3846
EaeI	YGGCCR	2 cut(s)	127, 3986
Eam1105I	GACNNNNNGTC	1 cut(s)	3846
EciI	GGCGGA	1 cut(s)	3770
Eco130I	CCWWGG	7 cut(s)	904, 1723, 2407, 2549, 2763, 3504, 4364
Eco24I	GRGCYC	1 cut(s)	548
Eco31I	GGTCTC	1 cut(s)	3225
Eco32I	GATATC	1 cut(s)	967
Eco47I	GGWCC	4 cut(s)	328, 1955, 2107, 4309
Eco57I	CTGAAG	3 cut(s)	945, 2191, 3609
Eco81I	CCTNAGG	1 cut(s)	4317
Eco88I	CYCGRG	1 cut(s)	1288
EcoNI	CCTNNNNNAGG	3 cut(s)	2673, 3213, 4316
EcoO109I	RGGNCCY	5 cut(s)	328, 1955, 3674, 4309, 4408
EcoRI	GAATTC	3 cut(s)	2143, 2782, 4512
EcoRII	CCWGG	8 cut(s)	1553, 2059, 2991, 3646, 3684, 3798, 4291, 4503
EcoRV	GATATC	1 cut(s)	967
EcoT14I	CCWWGG	7 cut(s)	904, 1723, 2407, 2549, 2763, 3504, 4364
EcoT22I	ATGCAT	1 cut(s)	4002
EcoT38I	GRGCYC	1 cut(s)	548
ErhI	CCWWGG	7 cut(s)	904, 1723, 2407, 2549, 2763, 3504, 4364
Esp3I	CGTCTC	2 cut(s)	1196, 2375
FaqI	GGGAC	3 cut(s)	1968, 2036, 2893
FauI	CCCGC	1 cut(s)	412
FbaI	TGATCA	2 cut(s)	847, 4228
FblI	GTMKAC	2 cut(s)	2987, 3069
FokI	GGATG	9 cut(s)	991, 1220, 1454, 2522, 3405, 3480, 3581, 4250, 4482
FriOI	GRGCYC	1 cut(s)	548
FspBI	CTAG	5 cut(s)	1047, 2363, 3285, 3368, 3902
GlaI	GCGC	1 cut(s)	645
GsaI	CCCAGC	1 cut(s)	2901
GsuI	CTGGAG	4 cut(s)	738, 2028, 3821, 3930
HaeIII	GGCC	9 cut(s)	129, 423, 1553, 2762, 3402, 3675, 3988, 4291, 4410
HapII	CCGG	4 cut(s)	999, 2659, 3467, 4038
HgaI	GACGC	1 cut(s)	4339
HhaI	GCGC	1 cut(s)	646
Hin6I	GCGC	1 cut(s)	644
HinP1I	GCGC	1 cut(s)	644
HincII	GTYRAC	1 cut(s)	1225
HindII	GTYRAC	1 cut(s)	1225
HindIII	AAGCTT	3 cut(s)	102, 2000, 2919
HpaII	CCGG	4 cut(s)	999, 2659, 3467, 4038
Hpy166II	GTNNAC	9 cut(s)	664, 1225, 2494, 2806, 2988, 3070, 3241, 3658, 4354
Hpy8I	GTNNAC	9 cut(s)	664, 1225, 2494, 2806, 2988, 3070, 3241, 3658, 4354
Hpy99I	CGWCG	2 cut(s)	1120, 1665
HpyCH4IV	ACGT	4 cut(s)	1096, 1115, 1660, 2384
HpySE526I	ACGT	4 cut(s)	1096, 1115, 1660, 2384
HspAI	GCGC	1 cut(s)	644
KroI	GCCGGC	1 cut(s)	3466
KroNI	GCCGGC	1 cut(s)	3468
Ksp22I	TGATCA	2 cut(s)	847, 4228
LguI	GCTCTTC	3 cut(s)	162, 2179, 3456
LmnI	GCTCC	8 cut(s)	1306, 1514, 1781, 2063, 2813, 3166, 3911, 4142
Lsp1109I	GCAGC	8 cut(s)	190, 193, 852, 1041, 1807, 2223, 3968, 4042
MaeI	CTAG	5 cut(s)	1047, 2363, 3285, 3368, 3902
MaeII	ACGT	4 cut(s)	1096, 1115, 1660, 2384
MaeIII	GTNAC	7 cut(s)	533, 1342, 1730, 2231, 3231, 3436, 4327
MbiI	CCGCTC	1 cut(s)	738
MfeI	CAATTG	2 cut(s)	603, 4113
MflI	RGATCY	6 cut(s)	5, 525, 2301, 2347, 2429, 4459
MhlI	GDGCHC	4 cut(s)	548, 1444, 4084, 4537
MlsI	TGGCCA	1 cut(s)	3988
MluNI	TGGCCA	1 cut(s)	3988
MlyI	GAGTC	6 cut(s)	260, 355, 902, 1280, 3509, 4615
MmeI	TCCRAC	5 cut(s)	955, 1284, 3508, 4404, 4643
Mox20I	TGGCCA	1 cut(s)	3988
Mph1103I	ATGCAT	1 cut(s)	4002
MroNI	GCCGGC	1 cut(s)	3466
MroXI	GAANNNNTTC	4 cut(s)	2402, 3321, 3707, 3972
MscI	TGGCCA	1 cut(s)	3988
MslI	CAYNNNNRTG	5 cut(s)	659, 1253, 2835, 3201, 3248
Msp20I	TGGCCA	1 cut(s)	3988
MspA1I	CMGCKG	3 cut(s)	865, 1798, 4130
MspCI	CTTAAG	1 cut(s)	3224
MspI	CCGG	4 cut(s)	999, 2659, 3467, 4038
MspR9I	CCNGG	9 cut(s)	1555, 2061, 2993, 3648, 3686, 3800, 4039, 4293, 4505
MunI	CAATTG	2 cut(s)	603, 4113
Mva1269I	GAATGC	3 cut(s)	1073, 2581, 3755
MvaI	CCWGG	8 cut(s)	1555, 2061, 2993, 3648, 3686, 3800, 4293, 4505
MvnI	CGCG	1 cut(s)	646
NaeI	GCCGGC	1 cut(s)	3468
NciI	CCSGG	1 cut(s)	4039
NcoI	CCATGG	5 cut(s)	904, 1723, 2407, 2549, 4364
NgoMIV	GCCGGC	1 cut(s)	3466
NlaIV	GGNNCC	7 cut(s)	7, 330, 1443, 1877, 1956, 4081, 4190
NmuCI	GTSAC	2 cut(s)	2231, 4327
NsiI	ATGCAT	1 cut(s)	4002
NspI	RCATGY	6 cut(s)	213, 1256, 2205, 2282, 2377, 4004
NspV	TTCGAA	4 cut(s)	1540, 3004, 4063, 4238
OliI	CACNNNNGTG	1 cut(s)	1253
PaeI	GCATGC	2 cut(s)	2205, 2282
PaeR7I	CTCGAG	1 cut(s)	1288
PagI	TCATGA	1 cut(s)	2830
PciI	ACATGT	2 cut(s)	1252, 2373
PciSI	GCTCTTC	3 cut(s)	162, 2179, 3456
PctI	GAATGC	3 cut(s)	1073, 2581, 3755
PdiI	GCCGGC	1 cut(s)	3468
PdmI	GAANNNNTTC	4 cut(s)	2402, 3321, 3707, 3972
PfeI	GAWTC	8 cut(s)	1925, 2051, 2905, 3281, 3350, 3708, 4200, 4279
PflFI	GACNNNGTC	1 cut(s)	4178
PflMI	CCANNNNNTGG	2 cut(s)	2941, 3723
PfoI	TCCNGGA	2 cut(s)	3798, 4037
PinAI	ACCGGT	1 cut(s)	998
PleI	GAGTC	6 cut(s)	259, 355, 901, 1280, 3508, 4615
PpsI	GAGTC	6 cut(s)	259, 355, 901, 1280, 3508, 4615
PpuMI	RGGWCCY	3 cut(s)	328, 1955, 4309
PscI	ACATGT	2 cut(s)	1252, 2373
PshBI	ATTAAT	3 cut(s)	1140, 3516, 3666
Psp5II	RGGWCCY	3 cut(s)	328, 1955, 4309
Psp6I	CCWGG	8 cut(s)	1553, 2059, 2991, 3646, 3684, 3798, 4291, 4503
PspFI	CCCAGC	1 cut(s)	2897
PspGI	CCWGG	8 cut(s)	1553, 2059, 2991, 3646, 3684, 3798, 4291, 4503
PspN4I	GGNNCC	7 cut(s)	7, 330, 1443, 1877, 1956, 4081, 4190
PspPI	GGNCC	7 cut(s)	328, 421, 1955, 2107, 3674, 4309, 4408
PspPPI	RGGWCCY	3 cut(s)	328, 1955, 4309
PstI	CTGCAG	1 cut(s)	2182
PstNI	CAGNNNCTG	1 cut(s)	1754
PsuI	RGATCY	6 cut(s)	5, 525, 2301, 2347, 2429, 4459
PsyI	GACNNNGTC	1 cut(s)	4178
PvuII	CAGCTG	3 cut(s)	865, 1798, 4130
RseI	CAYNNNNRTG	5 cut(s)	659, 1253, 2835, 3201, 3248
SapI	GCTCTTC	3 cut(s)	162, 2179, 3456
Sau96I	GGNCC	7 cut(s)	328, 421, 1955, 2107, 3674, 4309, 4408
ScaI	AGTACT	2 cut(s)	463, 4069
SchI	GAGTC	6 cut(s)	260, 355, 902, 1280, 3509, 4615
ScrFI	CCNGG	9 cut(s)	1555, 2061, 2993, 3648, 3686, 3800, 4039, 4293, 4505
SduI	GDGCHC	4 cut(s)	548, 1444, 4084, 4537
SfcI	CTRYAG	4 cut(s)	694, 2178, 3073, 4131
Sfr274I	CTCGAG	1 cut(s)	1288
SfuI	TTCGAA	4 cut(s)	1540, 3004, 4063, 4238
SinI	GGWCC	4 cut(s)	328, 1955, 2107, 4309
SlaI	CTCGAG	1 cut(s)	1288
SmiMI	CAYNNNNRTG	5 cut(s)	659, 1253, 2835, 3201, 3248
SmlI	CTYRAG	7 cut(s)	404, 1288, 1649, 3224, 3869, 4542, 4649
SmoI	CTYRAG	7 cut(s)	404, 1288, 1649, 3224, 3869, 4542, 4649
SphI	GCATGC	2 cut(s)	2205, 2282
SsiI	CCGC	6 cut(s)	9, 12, 419, 738, 3781, 3960
SspI	AATATT	3 cut(s)	1181, 1791, 2645
SspMI	CTAG	5 cut(s)	1047, 2363, 3285, 3368, 3902
StyD4I	CCNGG	9 cut(s)	1553, 2059, 2991, 3646, 3684, 3798, 4037, 4291, 4503
StyI	CCWWGG	7 cut(s)	904, 1723, 2407, 2549, 2763, 3504, 4364
TaiI	ACGT	4 cut(s)	1099, 1118, 1663, 2387
TaqII	GACCGA	1 cut(s)	2699
TatI	WGTACW	6 cut(s)	461, 1275, 1774, 2370, 2803, 4067
TauI	GCSGC	2 cut(s)	14, 3962
TfiI	GAWTC	8 cut(s)	1925, 2051, 2905, 3281, 3350, 3708, 4200, 4279
TseFI	GTSAC	2 cut(s)	2231, 4327
TseI	GCWGC	8 cut(s)	203, 206, 865, 1054, 1795, 2211, 3956, 4055
Tsp45I	GTSAC	2 cut(s)	2231, 4327
TspGWI	ACGGA	3 cut(s)	1220, 3836, 4079
Tth111I	GACNNNGTC	1 cut(s)	4178
Van91I	CCANNNNNTGG	2 cut(s)	2941, 3723
Vha464I	CTTAAG	1 cut(s)	3224
VpaK11BI	GGWCC	4 cut(s)	328, 1955, 2107, 4309
VspI	ATTAAT	3 cut(s)	1140, 3516, 3666
XagI	CCTNNNNNAGG	3 cut(s)	2673, 3213, 4316
XapI	RAATTY	9 cut(s)	746, 776, 1124, 1908, 1930, 2013, 2143, 2782, 4512
XbaI	TCTAGA	2 cut(s)	3284, 3901
XceI	RCATGY	6 cut(s)	213, 1256, 2205, 2282, 2377, 4004
XcmI	CCANNNNNNNNNTGG	3 cut(s)	3692, 4156, 4299
XhoI	CTCGAG	1 cut(s)	1288
XmiI	GTMKAC	2 cut(s)	2987, 3069
XmnI	GAANNNNTTC	4 cut(s)	2402, 3321, 3707, 3972
XspI	CTAG	5 cut(s)	1047, 2363, 3285, 3368, 3902
ZrmI	AGTACT	2 cut(s)	463, 4069
Zsp2I	ATGCAT	1 cut(s)	4002

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.