Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0002932

Physical Location & Seq

Genomic Coordinates Forward (+)

9295057 .. 9300452

Gene Structure 5396 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000016706

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4704 bp

ATGGGATCCGCCGCGGCCGCAGAAGCCGCCGCTGTTTCGAAGACCACCGCACCCAAAAACGACCCCAAATCGACCTCTTCTTCGTCTTCCTCAGGAATGACAAAAAAGAAGGGTAAAAAAGCTTCCCAAAAGGAAGCGCCTACCAGTGGTAGAGGTAAGAAGCGAAATTCGCCTCCGAGCGGTGAAGAGCAATCCCAGTCCCGCAAAATGCCCAAGCGTGCTGCTGCATGTAAAGACTTTAAGGAGAGGTCTGTTCGTCTTTCTGAAAAGTCTGATTTCATCGCAAGCAAGGAGGAACAGATTGTGTCAGATGAAAGTCTTGCCGTAGGCTTGACTTGTGGAAAGGATCCAGATGATGACAAGCCAAACAGAAGACTGACTCAGTTTATCTTGCATAATGAAGATGGTATTGCACAGCCACTTGAGATGGTGCAACATGGTGATCTGTTTATTACTGGCACTCTATTGCCTCTTGATGAAAGTTCTGTCAAGGGGAAGGTCAAGGACAGGGGTATCAAATGTGAAGGTTTTGGCCGAATAGAAGCATGGGACATCTCTGGTTATGATGAGGGACATCCAATAATATGGCTTACAACTGATGTTGCTGATTATTGTTGTGTTAAACCTGCAAGCAGCTACAAGAAACACTATGATAATTTCTGTGAGAAGGCGCGTGCTTGCATAGAGGTCTACAAAGTACTGTCAAAATGCAACTCTGACTGCAGTCTTGATGAATTACTTGCTGGCATTGCTCGATCAATGAGTGGAAACAAATTCTATGATGGCAGTGCTTCTATCAAAGAATTTATTCTATCTCAAGGTGTGTTTATCTATAATCAATTGGAAGGTTTGGAGGAGTCATCAAATAAGAATGATCGAATATTGGCACAGCTGCCTGTTCTCGCTGCCCTCAGAGAGGAGTGTATAAAGCATGGATATTATGTGCCCTCAAAACCAGCAGCTTCAAGTGGTACATTAAAGATTGCTTCAGATATCGGAGATGGACGGAATGGTTCACCCATAGTTGAAGCTGAGGAAGATGAAGATGCCAAGTTGGCTAGACTATTTCAAGAAGAAGAGTACTTCAAGTCTATGAAACAAAGAAAACGTCAGGTTGCTGCCTCCACGTCAAAGAAATTCTACATCAAAATTAATGAAGATGAAATTGCTAATGATTATCCTTTACCTGCCTATTATAAGACTGCTATTGACGAGACAGATGAATTTATAGTGGTCGACGGTGAGGATGGTGATCTTCCCACACACATGCTTCACAACTGGTGTCTATACAATTCAGACTCAAGACTGATTTCACTGGAGCTTCTTCCAATGAAGGCTTGTGCTGATATTGATGTTACAATTTTTGGTTCTGGGGTTATGAGTGAAGATGATGGAAGCAGTGGGTTTGATCTTGATGCTGACAGTATCCAGTCTTCTTCAAGTGGTTCAATAGCACCAGTAGCACAGGATGCTTATGGCATGCCAGTTTACTTGAGTTCCATAAAGGAATGGGTGATAGAATGGGGAGCTTCAATGATTTCAGTATCAATTCGAACAGAACTGGCCTGGTACAGACTTGGCAAGCCATCAAAGCAGTATGCTTTGTGGTATGAACCAATACTGAAAACAGCAAGGGTTGGGAGAAGTATAATCACAATGCTTAAGGCAGAAAGTCGGGTAGCACGGCTCTCTTTTGCAGATGTCATTAAAAGATTGTCAGAGTTACCTAAGAATGATGGTGGTTACATTTCTTCTGATCCAGCGTCTGTTGAGAGATATGTGGTCGTACATGGACCTATAATATTTCAGCTGTTTACAGAATTTCCAGATGTGAAGATTAAAAATTGTCCATTTGTTCATGGTCTTCGAGACAAAATGGAGAAGAGGCACCATACTAAATGGGTAGTAAATAAGAAGATTTTACAGAAGAGTGAATCAAATTTGAACCCAAGGGCATCAATGGGACCTGTGGTATCCAAGAAGAAGGCAATGCAGGCTACAACAACGAAGCTGATTAACAGGATCTGGGGGGACTACTATTCAAATTACTCCCCTGAAGATTCAAGCCCAGGAGCAACTTGTGAAAAGATAGAAGAGGAAGAAGTTGAAGAAGAGGCTCAAGAGGACATAGAAGAGGATGATGCAGAAGAGAATTCATCAGTAATGGAAAAAGCCCAAAGGCCTTCTCCAATTTTAAGAAAAATTAAATCATGCTCCAGCAGCAAGAAGATTTTGTGGGGTGAGAAGCCTGTGGGAGAAACATGTTCTGGTGAAGCTCTCTACAAGCATGCTGTTCTTCGTGGAGAAGAGATTTCTGTGGGTGGGGCTGTTTTGGTGGAAGTTGATGGTTCAGATGAACTTCCTGTTATCTATTTTGTAGAATACATGTATGAATCATCAGATGGAGAAAAAATGTTCCATGGCAGAATGATGCAGCGAGGATCTGATACTGTTCTTGGAAACACTGCTAATGAGAGGGAGCTATTCTTGACAAATGAGTGCATAAACTTGGCATTAAAGGATGTGAAACAGACAGTTGTTGTGGGTATCAAGTCAATGCCATGGGGGCATCAGTATAGGAAGGAGGATGCTGATGCTAATAGAATTGATAGAGCAAGAGCAGAAGAGAGGAAGAAGAAAGGATTGCCGACTGAATATTACTGTAGAAGCTTGTATTGCCCTGAGCAAGGTGCTTTCTTTAGTCTTTCTCGTCATACTATTGGTCTGGGCTCTGGTTTCTGCCACTCTTGCAAAGTCAATGAAGATGAGAAGGCCAAAGAAGTCTTCGAAGTGAATTCATCAAAGACTGGTTTTATATACAGTGGAGATGAGTACTCAGTTCATGATTATGTCTATGTAAGCCCCTATCAGTTTTCTGTGCAAACGATTGAAACTGAACTTTTCAAGGCTGGACGGAATCTGGGGTTGAAACCTTTTGTTGTGTGCCAAGTGCTGGGGATAATTACTGCAAAGGGATCTAAACAATCAGAAATGAAGTCTACCCAGGTTAAAGTGCGAAGATTTTTCAGACCAGAGGACATTTCGGTTGATAAAGCATACTGCTGTGATATTAGAGAGGTCTACTACAGTGAAGAACTACATATTTTGCCTGTAGATAGCATAGAAGGAAAATGTGAAGTCAGAAAGAGGAGTGATCTTCCAGAATGCAATGCTCCTGCATTGTTTCAGCACATATTCTTCTGTGAGCATCTATATGATCCTTCTAACGGGTCTATTAAGCAGTTGCCGGCTAACATCAGGGTAAAGTACTCAACAGTAGGTGGTGATCCTGAATCTAGAAAGAGAAAGGGCAAGTGTAAAGAAGGAGAAGATGTTCCAGAAGGTGAGAAACAGAGGGATGATTCTGGCCACAAACTCCTAGCCACTTTGGACATATTTGCTGGATGTGGTGGCTTATCGGAGGGGTTGCATAAGGCTGGTGTCTCAGTAACCAAGTGGGCAATCGAGTATGAAGAGCCGGCTGGACAGGCTTTCCAACTCAACCATCCCGAGTCCAAGGTGTTCATCAATAATTGCAATGTCATCTTGAAGGCCGTCATGGACAAATGTGGAGATATGGATGATTGCATCTCAACTGCTGATGCCAATGATTTAGCCGCATCACTAGATGAGAAGGTGAAAAATGATTTGCCCTTGCCAGGGCAAGTAGACTTTATTAATGGAGGCCCTCCATGCCAGGGTTTTTCTGGTATGAACAGATTCAACACCAGCACGTGGAGTAAAGTCCAGTGTGAAATGATATTGGCATTCTTATCCTTCGCTGATTACTTCCGGCCAAAATACTTCCTCCTGGAGAATGTGAGGAACTTTGTGTCTTTTAATAAAGGACAGACATTCCGTCTGACTGTGGCTTCACTTCTTGAGATGGGTTATCAGGTGAGGTTTGGTATTCTGGAAGCTGGAGCTTATGGGGTTTCCCAGTCCCGAAAGCGAGCATTTATTTGGGCAGCTGCACCAGATGAGATTCTTCCCGAATGGCCAGAGCCAATGCATGTTTTTGGTGTGCCGGAGTTGAAGATCAATTTATCTGGTAATTCATATTATGCTGCTGTTCGGAGTACTGCAAGTGGAGCCCCTTTCCGACCCATAACAGTAAGAGACACAATCGGCGATCTCCCAGCTGTAGGCAATGGAGCATCCAAGGTCAATCTGGAGTATGAAAGTGATCCTGTCTCGTGGTTCCAGAAGAAGATCAGAGGGAACATGGCTGTTTTGACTGATCACATTTCAAAAGAAATGAATGAGTTGAACCTCATTCGGTGCAAGAGGATTCCCAAGAGGCCTGGTGCAGACTGGAAGGACCTTCCAGAGGAAAAAGTGACGCTCTCTACTGGTCAAGTAGTTGACTTGATACCTTGGTGCCTGCCCAACACCGCCAAACGCCACAATCAGTGGAAGGGCCTGTTCGGAAGGTTGGACTGGGAAGGCAACTTCCCAACCTCCGTCACTGATCCTCAGCCAATGGGCAAGGTGGGAATGTGCTTCCATCCCGACCAGGATAGGATTATAACAGTTAGAGAATGCGCTCGTTCTCAAGGCTTTCCCGATAGCTACCAGTTTTATGGCAACGCTCTCCACAAGCACAGGCAGATTGGAAACGCTGTCCCTCCAACTCTGGCTTATGCTCTCGGCCGAAAACTCAAGGAAGCAGTCGACAGCAAGAGCTCTTCTTCACAAGAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000278 GO:0000281 GO:0000910 GO:0003006 GO:0003674 GO:0003824 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006349 GO:0006725 GO:0006807 GO:0007049 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008356 GO:0009292 GO:0009294 GO:0009790 GO:0009791 GO:0009793 GO:0009909 GO:0009910 GO:0009987 GO:0010069 GO:0010070 GO:0010154 GO:0010216 GO:0010424 GO:0010468 GO:0016740 GO:0016741 GO:0019222 GO:0022402 GO:0022414 GO:0032259 GO:0032501 GO:0032502 GO:0032776 GO:0034641 GO:0040029 GO:0043170 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044728 GO:0044764 GO:0046483 GO:0048316 GO:0048519 GO:0048580 GO:0048581 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0051093 GO:0051239 GO:0051241 GO:0051301 GO:0051704 GO:0060255 GO:0061458 GO:0061640 GO:0065007 GO:0071514 GO:0071704 GO:0090304 GO:1901360 GO:1903047 GO:2000026 GO:2000241 GO:2000242

KEGG Pathways

Metabolic & Signaling

ko00270 ko01100 ko05206 map00270 map01100 map05206

Pfam Domains

Protein Families

BAH DNA_methylase DNMT1-RFD

Protein Analysis

1568

Amino Acids

174.5

Weight (kDa)

5.95

Isoelectric Point (pI)

46.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DNMT1-RFD	PF12047	119 - 252	1.3e-33	Cytosine specific DNA methyltransferase replication foci domain
DNMT1-RFD	PF12047	418 - 574	7.4e-34	Cytosine specific DNA methyltransferase replication foci domain
BAH	PF01426	770 - 902	8.3e-21	BAH domain
BAH	PF01426	944 - 1083	5.5e-24	BAH domain
DNA_methylase	PF00145	1216 - 1555	6.5e-42	C-5 cytosine-specific DNA methylase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002932)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G08990 AT4G14140 AT4G14140 AT4G14140 AT5G49160 AT5G49160
fragaria_vesca	FvH4_1g10300 FvH4_1g10300
malus_domestica	MD02G1115100.v1.1 MD15G1231100.v1.1
prunus_persica	Prupe.7G060100_v2.0.a1 Prupe.7G183100_v2.0.a1 Prupe.7G183100_v2.0.a1
pyrus_communis	pycom02g09000 pycom02g09010 pycom15g20590 pycom15g20600
rosa_chinensis	RchiOBHm_Chr2g0097141 RchiOBHm_Chr5g0051581
rosa_laevigata	RLG00000016706 RLG00000034873
rosa_multiflora	Rmu_co8502141.1_g000001 Rmu_sc0001182.1_g000006 Rmu_ssc0000089.1_g000039
rosa_roxburghii	Rroxscaffold_1G00027090 Rroxscaffold_2G00144940
rosa_rugosa	Rorug02G0064600 Rorug05G0279000
rosa_samantha	Rh2AG111700 Rh2BG113700 Rh2CG116000 Rh2DG115000 Rh5AG350100 Rh5BG361900 Rh5CG386800 Rh5DG376800
rosa_wichuraiana	Rw2G008690 Rw5G033130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1197, 4529
Acc36I	ACCTGC	2 cut(s)	634, 1195
AccB1I	GGYRCC	2 cut(s)	1887, 4380
AccB7I	CCANNNNNTGG	2 cut(s)	2953, 3735
AccBSI	CCGCTC	1 cut(s)	180
AccI	GTMKAC	6 cut(s)	690, 1236, 2999, 3081, 3669, 4674
AccII	CGCG	2 cut(s)	14, 673
AcoI	YGGCCR	6 cut(s)	15, 532, 3367, 3794, 3998, 4651
AcsI	RAATTY	9 cut(s)	166, 773, 803, 1136, 1223, 1820, 1939, 2152, 2794
AcuI	CTGAAG	2 cut(s)	972, 2076
AcvI	CACGTG	1 cut(s)	3735
AfaI	GTAC	8 cut(s)	699, 973, 1082, 1571, 1788, 2834, 3269, 4081
AflII	CTTAAG	1 cut(s)	1661
AflIII	ACRYGT	2 cut(s)	2261, 2385
AhdI	GACNNNNNGTC	1 cut(s)	3858
AjiI	CACGTC	1 cut(s)	1128
AjnI	CCWGG	8 cut(s)	1565, 2068, 3003, 3658, 3696, 3810, 4303, 4515
AjuI	GAANNNNNNNTTGG	2 cut(s)	2768, 2800
AloI	GAACNNNNNNTCC	2 cut(s)	3318, 3350
Alw21I	GWGCWC	1 cut(s)	4688
Alw26I	GTCTC	5 cut(s)	1208, 1863, 3448, 4113, 4198
AlwNI	CAGNNNCTG	2 cut(s)	1766, 2025
Ama87I	CYCGRG	1 cut(s)	3509
ApoI	RAATTY	9 cut(s)	166, 773, 803, 1136, 1223, 1820, 1939, 2152, 2794
ArsI	GACNNNNNNTTYG	4 cut(s)	64, 96, 1093, 1125
AseI	ATTAAT	2 cut(s)	1152, 3678
Asp700I	GAANNNNTTC	3 cut(s)	807, 3333, 3719
AspLEI	GCGC	3 cut(s)	139, 673, 4547
AspS9I	GGNCC	5 cut(s)	1793, 1964, 3686, 4321, 4420
AsuII	TTCGAA	3 cut(s)	38, 1552, 2787
AvaI	CYCGRG	1 cut(s)	3509
AvaII	GGWCC	3 cut(s)	1793, 1964, 4321
AxyI	CCTNAGG	1 cut(s)	91
BaeGI	GKGCMC	1 cut(s)	948
BaeI	ACNNNNGTAYC	4 cut(s)	496, 529, 4364, 4397
BalI	TGGCCA	2 cut(s)	3369, 4000
BamHI	GGATCC	2 cut(s)	5, 346
BanI	GGYRCC	2 cut(s)	1887, 4380
BanII	GRGCYC	3 cut(s)	2732, 4096, 4688
BarI	GAAGNNNNNNTAC	4 cut(s)	106, 138, 1417, 1449
BauI	CACGAG	1 cut(s)	4195
BbrPI	CACGTG	1 cut(s)	3735
BbsI	GAAGAC	6 cut(s)	47, 78, 379, 1425, 1856, 2776
Bbv12I	GWGCWC	1 cut(s)	4688
BbvCI	CCTCAGC	2 cut(s)	1032, 4476
BceAI	ACGGC	3 cut(s)	308, 1700, 3539
BciT130I	CCWGG	8 cut(s)	1567, 2070, 3005, 3660, 3698, 3812, 4305, 4517
BciVI	GTATCC	2 cut(s)	1436, 1984
BclI	TGATCA	1 cut(s)	4240
BcoDI	GTCTC	5 cut(s)	1208, 1863, 3448, 4113, 4198
BfaI	CTAG	4 cut(s)	1059, 3297, 3380, 3626
BfmI	CTRYAG	5 cut(s)	721, 2662, 3085, 3111, 4143
BfoI	RGCGCY	1 cut(s)	140
BfrI	CTTAAG	1 cut(s)	1661
BfuAI	ACCTGC	2 cut(s)	634, 1195
BfuI	GTATCC	2 cut(s)	1436, 1984
BglI	GCCNNNNNGGC	2 cut(s)	1055, 2566
BmcAI	AGTACT	5 cut(s)	699, 1082, 2834, 3269, 4081
Bme1390I	CCNGG	8 cut(s)	1567, 2070, 3005, 3660, 3698, 3812, 4305, 4517
Bme18I	GGWCC	3 cut(s)	1793, 1964, 4321
BmeRI	GACNNNNNGTC	1 cut(s)	3858
BmeT110I	CYCGRG	1 cut(s)	3509
BmgBI	CACGTC	1 cut(s)	1128
BmgT120I	GGNCC	5 cut(s)	1793, 1964, 3686, 4321, 4420
BmiI	GGNNCC	7 cut(s)	7, 348, 1889, 1965, 4093, 4202, 4382
BmrFI	CCNGG	8 cut(s)	1567, 2070, 3005, 3660, 3698, 3812, 4305, 4517
BmrI	ACTGGG	3 cut(s)	190, 3934, 4450
BmuI	ACTGGG	3 cut(s)	190, 3934, 4450
BoxI	GACNNNNGTC	1 cut(s)	723
BpiI	GAAGAC	6 cut(s)	47, 78, 379, 1425, 1856, 2776
BpmI	CTGGAG	5 cut(s)	1337, 2200, 3833, 3942, 4193
Bpu10I	CCTNAGC	3 cut(s)	1032, 2682, 4476
Bpu14I	TTCGAA	3 cut(s)	38, 1552, 2787
BpuEI	CTTGAG	8 cut(s)	443, 801, 1285, 1513, 2103, 3902, 4539, 4646
BsaAI	YACGTR	1 cut(s)	3735
BsaBI	GATNNNNATC	1 cut(s)	1251
BsaXI	ACNNNNNCTCC	2 cut(s)	3318, 3348
Bse118I	RCCGGY	2 cut(s)	3247, 3478
Bse21I	CCTNAGG	1 cut(s)	91
Bse3DI	GCAATG	5 cut(s)	747, 1995, 3175, 3544, 4156
Bse8I	GATNNNNATC	1 cut(s)	1251
BseBI	CCWGG	8 cut(s)	1567, 2070, 3005, 3660, 3698, 3812, 4305, 4517
BseJI	GATNNNNATC	1 cut(s)	1251
BseMI	GCAATG	5 cut(s)	747, 1995, 3175, 3544, 4156
BseMII	CTCAG	8 cut(s)	105, 395, 925, 1023, 2673, 2850, 3459, 4490
BseRI	GAGGAG	3 cut(s)	869, 932, 3163
BseSI	GKGCMC	1 cut(s)	948
BseX3I	CGGCCG	2 cut(s)	15, 4651
BseYI	CCCAGC	2 cut(s)	2953, 4138
BsgI	GTGCAG	2 cut(s)	3957, 4329
Bsh1236I	CGCG	2 cut(s)	14, 673
Bsh1285I	CGRYCG	2 cut(s)	18, 4654
BshNI	GGYRCC	2 cut(s)	1887, 4380
BsiEI	CGRYCG	2 cut(s)	18, 4654
BsiHKAI	GWGCWC	1 cut(s)	4688
BsiHKCI	CYCGRG	1 cut(s)	3509
BsiSI	CCGG	4 cut(s)	3248, 3479, 3793, 4028
BslFI	GGGAC	7 cut(s)	184, 563, 585, 1977, 2045, 3928, 4610
BsmAI	GTCTC	5 cut(s)	1208, 1863, 3448, 4113, 4198
BsmFI	GGGAC	7 cut(s)	184, 563, 585, 1977, 2045, 3928, 4610
BsmI	GAATGC	3 cut(s)	3170, 3767, 4547
BsoBI	CYCGRG	1 cut(s)	3509
Bsp119I	TTCGAA	3 cut(s)	38, 1552, 2787
Bsp1286I	GDGCHC	4 cut(s)	948, 2732, 4096, 4688
Bsp19I	CCATGG	2 cut(s)	2419, 2561
BspCNI	CTCAG	8 cut(s)	104, 394, 924, 1024, 2674, 2849, 3458, 4489
BspFNI	CGCG	2 cut(s)	14, 673
BspHI	TCATGA	1 cut(s)	2842
BspLI	GGNNCC	7 cut(s)	7, 348, 1889, 1965, 4093, 4202, 4382
BspMAI	CTGCAG	1 cut(s)	725
BspMI	ACCTGC	2 cut(s)	634, 1195
BspQI	GCTCTTC	3 cut(s)	180, 3468, 4693
BspT104I	TTCGAA	3 cut(s)	38, 1552, 2787
BspT107I	GGYRCC	2 cut(s)	1887, 4380
BspTI	CTTAAG	1 cut(s)	1661
BsrBI	CCGCTC	1 cut(s)	180
BsrDI	GCAATG	5 cut(s)	747, 1995, 3175, 3544, 4156
BsrFI	RCCGGY	2 cut(s)	3247, 3478
BssAI	RCCGGY	2 cut(s)	3247, 3478
BssSI	CACGAG	1 cut(s)	4195
BssT1I	CCWWGG	6 cut(s)	1949, 2419, 2561, 3516, 4161, 4376
Bst2BI	CACGAG	1 cut(s)	4195
Bst2UI	CCWGG	8 cut(s)	1567, 2070, 3005, 3660, 3698, 3812, 4305, 4517
BstAFI	CTTAAG	1 cut(s)	1661
BstAPI	GCANNNNNTGC	1 cut(s)	1469
BstBAI	YACGTR	1 cut(s)	3735
BstBI	TTCGAA	3 cut(s)	38, 1552, 2787
BstDEI	CTNAG	9 cut(s)	91, 381, 911, 1032, 1728, 2682, 2836, 3445, 4476
BstDSI	CCRYGG	3 cut(s)	12, 2419, 2561
BstENI	CCTNNNNNAGG	3 cut(s)	914, 2685, 4328
BstFNI	CGCG	2 cut(s)	14, 673
BstH2I	RGCGCY	1 cut(s)	140
BstHHI	GCGC	3 cut(s)	139, 673, 4547
BstMAI	GTCTC	5 cut(s)	1208, 1863, 3448, 4113, 4198
BstMCI	CGRYCG	2 cut(s)	18, 4654
BstNI	CCWGG	8 cut(s)	1567, 2070, 3005, 3660, 3698, 3812, 4305, 4517
BstNSI	RCATGY	7 cut(s)	231, 1270, 1483, 2265, 2291, 2389, 4016
BstPAI	GACNNNNGTC	1 cut(s)	723
BstSCI	CCNGG	8 cut(s)	1565, 2068, 3003, 3658, 3696, 3810, 4303, 4515
BstSFI	CTRYAG	5 cut(s)	721, 2662, 3085, 3111, 4143
BstSLI	GKGCMC	1 cut(s)	948
BstUI	CGCG	2 cut(s)	14, 673
BstV2I	GAAGAC	6 cut(s)	47, 78, 379, 1425, 1856, 2776
BstX2I	RGATCY	5 cut(s)	5, 346, 2022, 2441, 2975
BstXI	CCANNNNNNTGG	2 cut(s)	585, 4583
BstYI	RGATCY	5 cut(s)	5, 346, 2022, 2441, 2975
BstZI	CGGCCG	2 cut(s)	15, 4651
Bsu36I	CCTNAGG	1 cut(s)	91
BsuI	GTATCC	2 cut(s)	1436, 1984
BtgI	CCRYGG	3 cut(s)	12, 2419, 2561
BtgZI	GCGATG	1 cut(s)	265
BtrI	CACGTC	1 cut(s)	1128
BtsI	GCAGTG	3 cut(s)	793, 1405, 2463
BveI	ACCTGC	2 cut(s)	634, 1195
CaiI	CAGNNNCTG	2 cut(s)	1766, 2025
CciI	TCATGA	1 cut(s)	2842
CciNI	GCGGCCGC	1 cut(s)	15
CfoI	GCGC	3 cut(s)	139, 673, 4547
Cfr10I	RCCGGY	2 cut(s)	3247, 3478
Cfr13I	GGNCC	5 cut(s)	1793, 1964, 3686, 4321, 4420
Cfr42I	CCGCGG	1 cut(s)	15
CseI	GACGC	2 cut(s)	1752, 4351
Csp6I	GTAC	8 cut(s)	698, 972, 1081, 1570, 1787, 2833, 3268, 4080
CviQI	GTAC	8 cut(s)	698, 972, 1081, 1570, 1787, 2833, 3268, 4080
DdeI	CTNAG	9 cut(s)	91, 381, 911, 1032, 1728, 2682, 2836, 3445, 4476
DriI	GACNNNNNGTC	1 cut(s)	3858
EaeI	YGGCCR	6 cut(s)	15, 532, 3367, 3794, 3998, 4651
EagI	CGGCCG	2 cut(s)	15, 4651
Eam1105I	GACNNNNNGTC	1 cut(s)	3858
Ecl136II	GAGCTC	1 cut(s)	4686
EclXI	CGGCCG	2 cut(s)	15, 4651
Eco130I	CCWWGG	6 cut(s)	1949, 2419, 2561, 3516, 4161, 4376
Eco147I	AGGCCT	2 cut(s)	2182, 4303
Eco24I	GRGCYC	3 cut(s)	2732, 4096, 4688
Eco32I	GATATC	1 cut(s)	994
Eco47I	GGWCC	3 cut(s)	1793, 1964, 4321
Eco52I	CGGCCG	2 cut(s)	15, 4651
Eco53kI	GAGCTC	1 cut(s)	4686
Eco57I	CTGAAG	2 cut(s)	972, 2076
Eco72I	CACGTG	1 cut(s)	3735
Eco81I	CCTNAGG	1 cut(s)	91
Eco88I	CYCGRG	1 cut(s)	3509
EcoICRI	GAGCTC	1 cut(s)	4686
EcoNI	CCTNNNNNAGG	3 cut(s)	914, 2685, 4328
EcoO109I	RGGNCCY	4 cut(s)	1964, 3686, 4321, 4420
EcoRI	GAATTC	2 cut(s)	2152, 2794
EcoRII	CCWGG	8 cut(s)	1565, 2068, 3003, 3658, 3696, 3810, 4303, 4515
EcoRV	GATATC	1 cut(s)	994
EcoT14I	CCWWGG	6 cut(s)	1949, 2419, 2561, 3516, 4161, 4376
EcoT22I	ATGCAT	1 cut(s)	4014
EcoT38I	GRGCYC	3 cut(s)	2732, 4096, 4688
ErhI	CCWWGG	6 cut(s)	1949, 2419, 2561, 3516, 4161, 4376
FaqI	GGGAC	7 cut(s)	184, 563, 585, 1977, 2045, 3928, 4610
FauI	CCCGC	1 cut(s)	209
FbaI	TGATCA	1 cut(s)	4240
FblI	GTMKAC	6 cut(s)	690, 1236, 2999, 3081, 3669, 4674
FriOI	GRGCYC	3 cut(s)	2732, 4096, 4688
FspBI	CTAG	4 cut(s)	1059, 3297, 3380, 3626
GlaI	GCGC	3 cut(s)	138, 672, 4546
GsaI	CCCAGC	2 cut(s)	2957, 4142
GsuI	CTGGAG	5 cut(s)	1337, 2200, 3833, 3942, 4193
HaeII	RGCGCY	1 cut(s)	140
HapII	CCGG	4 cut(s)	3248, 3479, 3793, 4028
HgaI	GACGC	2 cut(s)	1752, 4351
HhaI	GCGC	3 cut(s)	139, 673, 4547
Hin6I	GCGC	3 cut(s)	137, 671, 4545
HinP1I	GCGC	3 cut(s)	137, 671, 4545
HincII	GTYRAC	3 cut(s)	1237, 4366, 4675
HindII	GTYRAC	3 cut(s)	1237, 4366, 4675
HindIII	AAGCTT	2 cut(s)	120, 2668
HpaII	CCGG	4 cut(s)	3248, 3479, 3793, 4028
Hpy99I	CGWCG	1 cut(s)	1241
HpyCH4IV	ACGT	3 cut(s)	1108, 1127, 3734
HpyF3I	CTNAG	9 cut(s)	91, 381, 911, 1032, 1728, 2682, 2836, 3445, 4476
HpySE526I	ACGT	3 cut(s)	1108, 1127, 3734
HspAI	GCGC	3 cut(s)	137, 671, 4545
KroI	GCCGGC	2 cut(s)	3247, 3478
KroNI	GCCGGC	2 cut(s)	3249, 3480
Ksp22I	TGATCA	1 cut(s)	4240
KspI	CCGCGG	1 cut(s)	15
LguI	GCTCTTC	3 cut(s)	180, 3468, 4693
LmnI	GCTCC	9 cut(s)	1318, 1526, 2072, 2219, 2479, 3178, 3923, 4091, 4154
MaeI	CTAG	4 cut(s)	1059, 3297, 3380, 3626
MaeII	ACGT	3 cut(s)	1108, 1127, 3734
MaeIII	GTNAC	6 cut(s)	1354, 1722, 1742, 3448, 4339, 4465
MbiI	CCGCTC	1 cut(s)	180
MfeI	CAATTG	1 cut(s)	839
MflI	RGATCY	5 cut(s)	5, 346, 2022, 2441, 2975
MhlI	GDGCHC	4 cut(s)	948, 2732, 4096, 4688
MlsI	TGGCCA	2 cut(s)	3369, 4000
MluNI	TGGCCA	2 cut(s)	3369, 4000
MlyI	GAGTC	4 cut(s)	373, 866, 1292, 3521
MmeI	TCCRAC	4 cut(s)	3520, 4126, 4416, 4655
Mox20I	TGGCCA	2 cut(s)	3369, 4000
Mph1103I	ATGCAT	1 cut(s)	4014
MroNI	GCCGGC	2 cut(s)	3247, 3478
MroXI	GAANNNNTTC	3 cut(s)	807, 3333, 3719
MscI	TGGCCA	2 cut(s)	3369, 4000
MslI	CAYNNNNRTG	3 cut(s)	1265, 2847, 3213
Msp20I	TGGCCA	2 cut(s)	3369, 4000
MspA1I	CMGCKG	6 cut(s)	14, 32, 892, 1810, 3971, 4142
MspCI	CTTAAG	1 cut(s)	1661
MspI	CCGG	4 cut(s)	3248, 3479, 3793, 4028
MspR9I	CCNGG	8 cut(s)	1567, 2070, 3005, 3660, 3698, 3812, 4305, 4517
MunI	CAATTG	1 cut(s)	839
Mva1269I	GAATGC	3 cut(s)	3170, 3767, 4547
MvaI	CCWGG	8 cut(s)	1567, 2070, 3005, 3660, 3698, 3812, 4305, 4517
MvnI	CGCG	2 cut(s)	14, 673
NaeI	GCCGGC	2 cut(s)	3249, 3480
NcoI	CCATGG	2 cut(s)	2419, 2561
NgoMIV	GCCGGC	2 cut(s)	3247, 3478
NlaIV	GGNNCC	7 cut(s)	7, 348, 1889, 1965, 4093, 4202, 4382
NmeAIII	GCCGAG	1 cut(s)	4629
NmuCI	GTSAC	2 cut(s)	4339, 4465
NotI	GCGGCCGC	1 cut(s)	15
NsiI	ATGCAT	1 cut(s)	4014
NspI	RCATGY	7 cut(s)	231, 1270, 1483, 2265, 2291, 2389, 4016
NspV	TTCGAA	3 cut(s)	38, 1552, 2787
PaeI	GCATGC	2 cut(s)	1483, 2291
PagI	TCATGA	1 cut(s)	2842
PceI	AGGCCT	2 cut(s)	2182, 4303
PciI	ACATGT	2 cut(s)	2261, 2385
PciSI	GCTCTTC	3 cut(s)	180, 3468, 4693
PctI	GAATGC	3 cut(s)	3170, 3767, 4547
PdiI	GCCGGC	2 cut(s)	3249, 3480
PdmI	GAANNNNTTC	3 cut(s)	807, 3333, 3719
PfeI	GAWTC	9 cut(s)	1934, 2060, 2393, 2917, 3293, 3362, 3720, 3985, 4291
PflMI	CCANNNNNTGG	2 cut(s)	2953, 3735
PfoI	TCCNGGA	1 cut(s)	3810
PleI	GAGTC	4 cut(s)	373, 865, 1292, 3520
PmaCI	CACGTG	1 cut(s)	3735
PmlI	CACGTG	1 cut(s)	3735
PpsI	GAGTC	4 cut(s)	373, 865, 1292, 3520
Ppu21I	YACGTR	1 cut(s)	3735
PpuMI	RGGWCCY	2 cut(s)	1964, 4321
PscI	ACATGT	2 cut(s)	2261, 2385
PshAI	GACNNNNGTC	1 cut(s)	723
PshBI	ATTAAT	2 cut(s)	1152, 3678
PsiI	TTATAA	2 cut(s)	1197, 4529
Psp124BI	GAGCTC	1 cut(s)	4688
Psp5II	RGGWCCY	2 cut(s)	1964, 4321
Psp6I	CCWGG	8 cut(s)	1565, 2068, 3003, 3658, 3696, 3810, 4303, 4515
PspCI	CACGTG	1 cut(s)	3735
PspFI	CCCAGC	2 cut(s)	2953, 4138
PspGI	CCWGG	8 cut(s)	1565, 2068, 3003, 3658, 3696, 3810, 4303, 4515
PspN4I	GGNNCC	7 cut(s)	7, 348, 1889, 1965, 4093, 4202, 4382
PspPI	GGNCC	5 cut(s)	1793, 1964, 3686, 4321, 4420
PspPPI	RGGWCCY	2 cut(s)	1964, 4321
PstI	CTGCAG	1 cut(s)	725
PstNI	CAGNNNCTG	2 cut(s)	1766, 2025
PsuI	RGATCY	5 cut(s)	5, 346, 2022, 2441, 2975
PvuII	CAGCTG	4 cut(s)	892, 1810, 3971, 4142
RsaI	GTAC	8 cut(s)	699, 973, 1082, 1571, 1788, 2834, 3269, 4081
RsaNI	GTAC	8 cut(s)	698, 972, 1081, 1570, 1787, 2833, 3268, 4080
RseI	CAYNNNNRTG	3 cut(s)	1265, 2847, 3213
SacI	GAGCTC	1 cut(s)	4688
SacII	CCGCGG	1 cut(s)	15
SalI	GTCGAC	2 cut(s)	1235, 4673
SapI	GCTCTTC	3 cut(s)	180, 3468, 4693
Sau96I	GGNCC	5 cut(s)	1793, 1964, 3686, 4321, 4420
ScaI	AGTACT	5 cut(s)	699, 1082, 2834, 3269, 4081
SchI	GAGTC	4 cut(s)	373, 866, 1292, 3521
ScrFI	CCNGG	8 cut(s)	1567, 2070, 3005, 3660, 3698, 3812, 4305, 4517
SduI	GDGCHC	4 cut(s)	948, 2732, 4096, 4688
SfcI	CTRYAG	5 cut(s)	721, 2662, 3085, 3111, 4143
Sfr303I	CCGCGG	1 cut(s)	15
SfuI	TTCGAA	3 cut(s)	38, 1552, 2787
SgrBI	CCGCGG	1 cut(s)	15
SinI	GGWCC	3 cut(s)	1793, 1964, 4321
SmiMI	CAYNNNNRTG	3 cut(s)	1265, 2847, 3213
SmlI	CTYRAG	9 cut(s)	422, 816, 1300, 1492, 1661, 2118, 3881, 4554, 4661
SmoI	CTYRAG	9 cut(s)	422, 816, 1300, 1492, 1661, 2118, 3881, 4554, 4661
SphI	GCATGC	2 cut(s)	1483, 2291
SseBI	AGGCCT	2 cut(s)	2182, 4303
SspI	AATATT	3 cut(s)	882, 1803, 2657
SspMI	CTAG	4 cut(s)	1059, 3297, 3380, 3626
SstI	GAGCTC	1 cut(s)	4688
StuI	AGGCCT	2 cut(s)	2182, 4303
StyD4I	CCNGG	8 cut(s)	1565, 2068, 3003, 3658, 3696, 3810, 4303, 4515
StyI	CCWWGG	6 cut(s)	1949, 2419, 2561, 3516, 4161, 4376
TaiI	ACGT	3 cut(s)	1111, 1130, 3737
TatI	WGTACW	5 cut(s)	697, 1080, 2832, 3267, 4079
TauI	GCSGC	6 cut(s)	14, 17, 20, 29, 32, 3620
TfiI	GAWTC	9 cut(s)	1934, 2060, 2393, 2917, 3293, 3362, 3720, 3985, 4291
TseFI	GTSAC	2 cut(s)	4339, 4465
Tsp45I	GTSAC	2 cut(s)	4339, 4465
TspGWI	ACGGA	4 cut(s)	1021, 2929, 3848, 4453
Van91I	CCANNNNNTGG	2 cut(s)	2953, 3735
Vha464I	CTTAAG	1 cut(s)	1661
VpaK11BI	GGWCC	3 cut(s)	1793, 1964, 4321
VspI	ATTAAT	2 cut(s)	1152, 3678
XagI	CCTNNNNNAGG	3 cut(s)	914, 2685, 4328
XapI	RAATTY	9 cut(s)	166, 773, 803, 1136, 1223, 1820, 1939, 2152, 2794
XbaI	TCTAGA	1 cut(s)	3296
XceI	RCATGY	7 cut(s)	231, 1270, 1483, 2265, 2291, 2389, 4016
XcmI	CCANNNNNNNNNTGG	2 cut(s)	3704, 4168
XmiI	GTMKAC	6 cut(s)	690, 1236, 2999, 3081, 3669, 4674
XmnI	GAANNNNTTC	3 cut(s)	807, 3333, 3719
XspI	CTAG	4 cut(s)	1059, 3297, 3380, 3626
ZrmI	AGTACT	5 cut(s)	699, 1082, 2834, 3269, 4081
Zsp2I	ATGCAT	1 cut(s)	4014

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.