Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0002932

Physical Location & Seq

Genomic Coordinates Forward (+)

9352647 .. 9358321

Gene Structure 5675 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1115100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4713 bp

ATGGGTTCCGCCGCGGCAGCTGAGGCAGCGGAAGCAGCGGCGGTCTTGGAGACCAAAGGTGCCAATGGGGTTAAGCCACCGTCCTCGGCTTCCTCAGGAACGACGAAGAAGAAGAGCAAACCAGCTTCCCAAAAGGCCGCACCTGCCGGCTCTAAAACTAAGAAGCGAGATTTGCCTCAGAACACTGAAGAGCCTTCCAGGTCTCGGAAAATGCCCAAGCGGGCTGCTGCATGTGCAGACTTTAAGGAGAAGTCTGTTCATATTTCTGAAAAGTCTAATCTTATTGAAACCAAGAAGGATCGAGTAGTGGATCAAGAAACTGATGCTATACTCCTGACTTGTGGCGAGGACCAAGATGCGGCGCGCCCAAACAGAAGGCTGACTGATTTTATTGTGCATGATGAAAGTGGGTCACCCCAGCCCGTTGAGATGTTGGAAGTTGCTGACATGTTTATATCTGGTACTATATTGCCTCTGAATGAAAGTTCTGACAAGGACAAGCAAAGAGGTGTTAGATGTGAAGGTTTTGGGCGAATAGAATCTTGGGACATCTCTGGTTATGAAGAGGGCTCTCCTGTGATATGGCTCTCAACTGATGTTGCTGATTATGATTGTCGTAAACCTGCCAGCACCTATAGGAAGTACCATGATCAATTCTTTGAGAAAGCGCGTGCTTGCATAGAGGTTTACAAAAAGCTGTCAAAATCCAAATCTGACCCCACTCTTGATGAATTACTTGCTGGCATTGCTCGATCAATGAGTGGAAGCAAATTCTTTTCTGGTACTGCAGCTATCAAAGAATTTGTTGTATCTCAAGGCGAGTTTATCTATGATCAACTGATAGGTTTGGAGGAAGCATCAAAGAAGAATGATCAGCCATTTGCAGAGCTTCCTGTTCTTGTTGCCCTTAGAGATGAGAGCAGAAACTGTGGAGGTTTTGTCCAATCAAAACCAGCAAGTTCAAGTGGTACCCTAAAGATTGGTTCAGAAGATAGAGATGGAGAGACTGTTCTGAATGCATCCGGTTCATCCATAGTTGAAGCTGAGGAAAATGATGATGTAAAATTGGCAAGACTTTTGCAAGAAGAGGAGTACTGGAAGTCAATGAAACAGAAAAAACGTCAGGGTTCTGCCTCTTTGTCGAGCAAATACTATATCAAAATCAATGAAGATGAAATTGCCAACGATTATCCTCTACCTGCTTATTACAAGACTTCCATTGAAGAAACTGACGAGTTCATAGTTTTTGACAATGACTATGATATCTTAAATGCTGATGACCTTCCTCGAAGCATGCTTCATAATTGGAGTCTGTACAATTCAGACTCAAGATTGATTTCGTTGGAACTTCTTCCGATGAAACCTTGCACAGAAATTGATGTTACCATTTTCGGGTCTGGGGTTATGACTGCAGATGATGGAAGCGGCTTTTCTCTTGATTCTGATGGTTCTTCAAGTGGTCCCGGAGCACAGGATGCTGATGGAATGCCGATTTACTTGAGTGCAATAAAGGAGTGGATGATTGAATTGGGAGCATCAATGGTCTCAATATCAATCCGAACAGATTTGGCCTGGTACAGACTTGGACAGCCATCTAAGCAGTATGCTCTGTGGTATGAACCAATTCTGAAAACAGCAAAGGTCGGTAGAAGCATAATCACTTTGCTGAAAGAGCAAAGTCGAGTAGCACGACTTTCTTTTGCAGATGTTATTAAGAGATTGTCAGGGTTTCCAAAGGACCACTGTGCTTACATTTCTTCCGATCCAGCATTTGTTGAGAGATATGTGGTTGTCCATGGACAGATAATACTGCAACTGTTTTCAGAATTTCCTGATGCGCAGATTAAAAAGTGTCCATTTGTGGTTGGTCTTTCAAACAAAATGGAGGAGAGGCACCATACTAAATGGTTGGTAAAGAAGAAGAAGCTTGTGGAGAAGAGTGGATCAAACTTGAACCCAAGGGCATCCATGGGACCTGTGGTTTCCAAGAAGAAGGCAATGCGGGCTACAACAACAAAGCTGATCAACAGAATCTGGGGGGAGTACTATTTAAATAATTCTCCAGAAGATTCGAATGAAGAGGAAACTAATGGTGGAAAGAAAGAGGAGGAGGAAGTTGAAGAAGAGGAGGGAAAAGAGGATGTAGAAGAGGATGAAGATGATGAGAAAGATAATCCAACGGAACAAGCCCAGAAGCGTAGTTCAATTTCAAGACAAACTAAATCATGCTCCAACAACAAGGAGGTTCTGTGGGATGGGGAGTCTGTGGGAACAACGTGTTCTGGTGAAGCTCTTTATAAGTGTGCCAGTCTTCATGGAGATGAAATTTCTGTTGGTGGTGCAGTCTTGGTAGAACTTGATGGATCAGATGAACTTCCTGCCATTTATTTTGTTGAGTATATGTATGAAACAAGAAATGGAAGCAAAATGTTCCATGGGAGATTGATGGAGCGAGGGTCTCAGACTGTTCTTGGCAACACTGCCAATGAGAGGGAGGTATTTTTGACAAATGAGTGCACAAACTTGGCATTAAAGGATGTTAAAGAGACAGCAGTTGTGGATATCAAATTAATGCCGTGGGGGCATCAATACAGGAAGGAGAATGCAGAAGCTAGCCGAAGAGATAGAGAAAGGGCAGAAGATAGGAAGAAGAAGGGATTGCCAACTGAATATTACTGTAAAAGCTTGTATTGCCCTGAGGAAGGTGCTTTCTTTAGCCTTTCCCGTGATACCATGGGTCTGGGTTCTGGTGCCTGCCACTCTTGCAAAGTGAACGAAGCTGAAGAGGCCAAGGAGGTTTTTAAAGTGAATTCATCAAAGACTGGTTTTGTATACAGGGGAGCCGAGTACTCAGTTCACGATTATGTCTATGTAAGTCCCCATTTGTTTTCTACGGAAAGGATGGAAACTGAAACTTTCAAGGCTGGTAGAAATTTGGGACTGAAAGCTTATGTTGTGTGCCAAGTGCTGGAGATAATTGGTACGAAGGAATCGAAACGACCTGGACCAGTATCTACCCAGGTTAAAGTTAGAAGGTTTTTCAGACCAGAGGACATTTCGGTTGAGAAGGCATACGGTTGTGATATTAGAGAGGTCTACTACAGTGAAGAAACACACATTGTGACGGTTGATGATATAGAAGGAAAATGTGAAGTCAGAAAGAAGAGTGATCTTCCAGTATGCAATGCTCCTGTCACTTTCGAGCATACCTTCTTCTGTGAGTATCTGTACGATCCTTCTAATGGGTCTATCAAGCAGTTGCCAGCGACCATCAAACTGAGGTACTCAACAGTAGGTGGTGATGTGGAATCTAGGAAGAGGAAGGGGAAAGGCAAAGAAGGAGATGTTTCAGAAGTTGAGAAACAGAGAGCTGATTCTGTGCAGAAACGTCTAGCCACATTAGATATATTTGCCGGGTGTGGTGGCTTGTCTGAAGGGTTGCGTCAGGCTGGCATTTCATTAACCAAGTGGGCAATTGAGTACGAAGAGCCTGCTGGTGATGCTTTCAAACTGAACCATCCCGAGTCACTGGTGTTTATCAATAACTGCAATGTGATCTTAAGGGCTGTAATGGAGAAATGCGGGGATACAGATGATTGCATCTCGACTTCCGAAGCTGCTGATTTGGCTAAATCACTTGATGAGAAGGTTAAGAATGATCTGCCACTGCCGGGGCAAGTGGATTTCATCAATGGAGGACCTCCGTGTCAGGGTTTCTCTGGAATGAATAGGTTCAACCAAAGCACTTGGAGTAAAGTTCAGTGTGAAATGATTTTGGCATTTTTATCCTTTGCCGATTACTTCCGGCCAAAGTATTTCCTCTTGGAGAATGTGAGGAACTTTGTGTCTTTCAACAAAGGACAGACATTCCGTCTGACTGTGGCTTCACTTCTTGAGATGGGTTACCAGGTGAGGTTCGGTATTCTAGAAGCTGGAGCTTATGGAGTTTCTCAGTCACGAAAGCGAGCATTCATATGGGCAGCTGCGCCAGATGAGAATCTCCCAGAGTGGCCAGAGCCAATGCATGTCTTTGGTGTACCGGAGTTGAAGATCTCATTGTCAGGGAACTCATATTACTCTGCGGTTAGAAGTACTGCAGGTGGGGCTCCTTTCCGTTCCATAACTGTCAGAGACACAATTGGAGATCTCCCAGCTGTAGGGAATGGAGCATCCAAGGTCAATCTGGAGTACGAAAGCGACCCCATATCATGGTTCCAGAAGAAAATCCGAGGGGAGATGGCTGTTCTGACTGAGCACATTTCGAAAGAAATGAACGAGCTGAATCTCATTCGATGCCAGAGGATTCCAAAGCGGCCAGGCGCTGATTGGCAATGTCTTCCAGACGAAAAGGTGAAGCTCTCCACTGGACAGATAGTTGACTTGATCCCATGGTGCCTTCCCAACACAGCCAAGCGCCACAATCAGTGGAAGGGCCTGTTCGGAAGGCTTGACTGGGAAGGCAACTTCCCAACCTCCATCACAGATCCTCAACCAATGGGGAAGGTGGGAATGTGCTTCCACCCTGACCAGGACAGGATTCTGACAGTTCGGGAATGCGCTCGGTCTCAAGGATTCAGGGATAGCTACCAGTTTTCGGGCAACATTCTTCACAAGCACAGGCAGATTGGAAATGCAGTTCCTCCTACTTTGGCCTATGCGTTGGGACGAAAACTCAAGGAAGCAGTGAACAGCAAGAACAAGGAACAGGAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000278 GO:0000281 GO:0000910 GO:0003006 GO:0003674 GO:0003824 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006349 GO:0006725 GO:0006807 GO:0007049 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008356 GO:0009292 GO:0009294 GO:0009790 GO:0009791 GO:0009793 GO:0009909 GO:0009910 GO:0009987 GO:0010069 GO:0010070 GO:0010154 GO:0010216 GO:0010424 GO:0010468 GO:0016740 GO:0016741 GO:0019222 GO:0022402 GO:0022414 GO:0032259 GO:0032501 GO:0032502 GO:0032776 GO:0034641 GO:0040029 GO:0043170 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044728 GO:0044764 GO:0046483 GO:0048316 GO:0048519 GO:0048580 GO:0048581 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0051093 GO:0051239 GO:0051241 GO:0051301 GO:0051704 GO:0060255 GO:0061458 GO:0061640 GO:0065007 GO:0071514 GO:0071704 GO:0090304 GO:1901360 GO:1903047 GO:2000026 GO:2000241 GO:2000242

KEGG Pathways

Metabolic & Signaling

ko00270 ko01100 ko05206 map00270 map01100 map05206

Pfam Domains

Protein Families

BAH DNA_methylase DNMT1-RFD

Protein Analysis

1571

Amino Acids

175.1

Weight (kDa)

5.66

Isoelectric Point (pI)

44.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DNMT1-RFD	PF12047	121 - 251	2.4e-37	Cytosine specific DNA methyltransferase replication foci domain
DNMT1-RFD	PF12047	426 - 576	6.6e-35	Cytosine specific DNA methyltransferase replication foci domain
BAH	PF01426	775 - 907	6.6e-18	BAH domain
BAH	PF01426	949 - 1088	8.7e-25	BAH domain
DNA_methylase	PF00145	1220 - 1559	1.1e-40	C-5 cytosine-specific DNA methylase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002932)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G08990 AT4G14140 AT4G14140 AT4G14140 AT5G49160 AT5G49160
fragaria_vesca	FvH4_1g10300 FvH4_1g10300
malus_domestica	MD02G1115100.v1.1 MD15G1231100.v1.1
prunus_persica	Prupe.7G060100_v2.0.a1 Prupe.7G183100_v2.0.a1 Prupe.7G183100_v2.0.a1
pyrus_communis	pycom02g09000 pycom02g09010 pycom15g20590 pycom15g20600
rosa_chinensis	RchiOBHm_Chr2g0097141 RchiOBHm_Chr5g0051581
rosa_laevigata	RLG00000016706 RLG00000034873
rosa_multiflora	Rmu_co8502141.1_g000001 Rmu_sc0001182.1_g000006 Rmu_ssc0000089.1_g000039
rosa_roxburghii	Rroxscaffold_1G00027090 Rroxscaffold_2G00144940
rosa_rugosa	Rorug02G0064600 Rorug05G0279000
rosa_samantha	Rh2AG111700 Rh2BG113700 Rh2CG116000 Rh2DG115000 Rh5AG350100 Rh5BG361900 Rh5CG386800 Rh5DG376800
rosa_wichuraiana	Rw2G008690 Rw5G033130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2298
AarI	CACCTGC	2 cut(s)	151, 4088
AasI	GACNNNNNNGTC	1 cut(s)	3705
Acc16I	TGCGCA	1 cut(s)	1839
Acc36I	ACCTGC	4 cut(s)	151, 631, 1207, 4088
Acc65I	GGTACC	1 cut(s)	968
AccB1I	GGYRCC	5 cut(s)	59, 968, 1893, 2750, 4392
AccB7I	CCANNNNNTGG	2 cut(s)	2968, 4209
AccI	GTMKAC	2 cut(s)	2832, 3096
AccII	CGCG	3 cut(s)	14, 364, 670
AclWI	GGATC	8 cut(s)	306, 318, 1757, 1951, 2371, 3227, 4378, 4478
AcoI	YGGCCR	3 cut(s)	3806, 4010, 4313
AcsI	RAATTY	6 cut(s)	770, 800, 1826, 2325, 2809, 2932
AcuI	CTGAAG	3 cut(s)	207, 2802, 3453
AdeI	CACNNNGTG	1 cut(s)	3121
AflII	CTTAAG	1 cut(s)	3559
AflIII	ACRYGT	2 cut(s)	447, 2276
AhdI	GACNNNNNGTC	1 cut(s)	3870
AjnI	CCWGG	7 cut(s)	197, 1571, 3001, 3018, 3906, 4315, 4527
AjuI	GAANNNNNNNTTGG	4 cut(s)	526, 558, 2316, 2348
AloI	GAACNNNNNNTCC	4 cut(s)	993, 1025, 4620, 4652
Alw21I	GWGCWC	3 cut(s)	1471, 2519, 4257
Alw26I	GTCTC	8 cut(s)	44, 207, 998, 1549, 2463, 2540, 4125, 4569
Alw44I	GTGCAC	1 cut(s)	2515
AlwI	GGATC	8 cut(s)	306, 318, 1757, 1951, 2371, 3227, 4378, 4478
AlwNI	CAGNNNCTG	3 cut(s)	927, 2034, 4322
Ama87I	CYCGRG	1 cut(s)	3521
AoxI	GGCC	8 cut(s)	135, 1569, 2787, 3806, 4010, 4313, 4432, 4650
ApaLI	GTGCAC	1 cut(s)	2515
ApeKI	GCWGC	9 cut(s)	17, 26, 35, 224, 227, 788, 3617, 3980, 3983
ApoI	RAATTY	6 cut(s)	770, 800, 1826, 2325, 2809, 2932
AscI	GGCGCGCC	1 cut(s)	362
AseI	ATTAAT	1 cut(s)	2570
Asp700I	GAANNNNTTC	3 cut(s)	1350, 1623, 3731
Asp718I	GGTACC	1 cut(s)	968
AspLEI	GCGC	8 cut(s)	364, 366, 670, 1840, 3988, 4322, 4416, 4559
AspS9I	GGNCC	7 cut(s)	349, 1460, 1738, 1973, 3005, 3698, 4432
AsuC2I	CCSGG	3 cut(s)	1464, 3415, 3672
AsuHPI	GGTGA	6 cut(s)	405, 2297, 3311, 3509, 3922, 4363
AsuII	TTCGAA	2 cut(s)	2072, 4262
AsuNHI	GCTAGC	1 cut(s)	2612
AvaI	CYCGRG	1 cut(s)	3521
AvaII	GGWCC	6 cut(s)	349, 1460, 1738, 1973, 3005, 3698
AxyI	CCTNAGG	2 cut(s)	94, 2697
BaeGI	GKGCMC	1 cut(s)	2519
BalI	TGGCCA	1 cut(s)	4012
BanI	GGYRCC	5 cut(s)	59, 968, 1893, 2750, 4392
BanII	GRGCYC	2 cut(s)	572, 4108
BbsI	GAAGAC	2 cut(s)	2303, 4328
Bbv12I	GWGCWC	3 cut(s)	1471, 2519, 4257
BbvCI	CCTCAGC	2 cut(s)	21, 1044
BbvI	GCAGC	9 cut(s)	29, 38, 47, 211, 214, 800, 3604, 3970, 3992
BceAI	ACGGC	1 cut(s)	2560
BcgI	CGANNNNNNTGC	2 cut(s)	3473, 3507
BciT130I	CCWGG	7 cut(s)	199, 1573, 3003, 3020, 3908, 4317, 4529
BciVI	GTATCC	1 cut(s)	3580
BclI	TGATCA	4 cut(s)	649, 832, 871, 2022
BcnI	CCSGG	3 cut(s)	1464, 3415, 3672
BcoDI	GTCTC	8 cut(s)	44, 207, 998, 1549, 2463, 2540, 4125, 4569
BfaI	CTAG	4 cut(s)	2613, 3312, 3392, 3926
BfmI	CTRYAG	6 cut(s)	634, 786, 1410, 3100, 4095, 4155
BfoI	RGCGCY	2 cut(s)	4323, 4417
BfrI	CTTAAG	1 cut(s)	3559
BfuAI	ACCTGC	4 cut(s)	151, 631, 1207, 4088
BfuI	GTATCC	1 cut(s)	3580
BglI	GCCNNNNNGGC	1 cut(s)	2581
BglII	AGATCT	2 cut(s)	4050, 4144
BmcAI	AGTACT	4 cut(s)	1094, 2045, 2849, 4093
Bme18I	GGWCC	6 cut(s)	349, 1460, 1738, 1973, 3005, 3698
BmeRI	GACNNNNNGTC	1 cut(s)	3870
BmeT110I	CYCGRG	1 cut(s)	3521
BmgT120I	GGNCC	7 cut(s)	349, 1460, 1738, 1973, 3005, 3698, 4432
BmrI	ACTGGG	1 cut(s)	4462
BmtI	GCTAGC	1 cut(s)	2616
BmuI	ACTGGG	1 cut(s)	4462
BpiI	GAAGAC	2 cut(s)	2303, 4328
BpmI	CTGGAG	4 cut(s)	2046, 2990, 3954, 4205
Bpu10I	CCTNAGC	2 cut(s)	21, 1044
Bpu14I	TTCGAA	2 cut(s)	2072, 4262
BpuEI	CTTGAG	6 cut(s)	798, 1312, 1519, 3914, 4551, 4658
BpuMI	CCSGG	3 cut(s)	1464, 3415, 3672
BsaBI	GATNNNNATC	1 cut(s)	3996
BsaI	GGTCTC	5 cut(s)	44, 207, 1549, 2463, 4569
BsaWI	WCCGGW	2 cut(s)	1022, 4039
BsaXI	ACNNNNNCTCC	8 cut(s)	924, 954, 993, 1023, 2234, 2264, 2441, 2471
Bse118I	RCCGGY	1 cut(s)	146
Bse1I	ACTGG	9 cut(s)	1100, 2307, 2827, 3008, 3176, 3534, 4369, 4457, 4588
Bse21I	CCTNAGG	2 cut(s)	94, 2697
Bse3DI	GCAATG	5 cut(s)	744, 2004, 3190, 3556, 4337
Bse8I	GATNNNNATC	1 cut(s)	3996
BseBI	CCWGG	7 cut(s)	199, 1573, 3003, 3020, 3908, 4317, 4529
BseJI	GATNNNNATC	1 cut(s)	3996
BseMI	GCAATG	5 cut(s)	744, 2004, 3190, 3556, 4337
BseNI	ACTGG	9 cut(s)	1100, 2307, 2827, 3008, 3176, 3534, 4369, 4457, 4588
BsePI	GCGCGC	1 cut(s)	362
BseRI	GAGGAG	5 cut(s)	1103, 1901, 2120, 2123, 2141
BseSI	GKGCMC	1 cut(s)	2519
BseXI	GCAGC	9 cut(s)	29, 38, 47, 211, 214, 800, 3604, 3970, 3992
BseYI	CCCAGC	2 cut(s)	417, 4150
BsgI	GTGCAG	3 cut(s)	255, 2361, 3401
Bsh1236I	CGCG	3 cut(s)	14, 364, 670
BshFI	GGCC	8 cut(s)	137, 1571, 2789, 3808, 4012, 4315, 4434, 4652
BshNI	GGYRCC	5 cut(s)	59, 968, 1893, 2750, 4392
BsiHKAI	GWGCWC	3 cut(s)	1471, 2519, 4257
BsiHKCI	CYCGRG	1 cut(s)	3521
BsiSI	CCGG	7 cut(s)	147, 1023, 1464, 3414, 3671, 3805, 4040
BslFI	GGGAC	6 cut(s)	560, 1446, 1986, 2862, 2952, 4677
BsmAI	GTCTC	8 cut(s)	44, 207, 998, 1549, 2463, 2540, 4125, 4569
BsmFI	GGGAC	6 cut(s)	560, 1446, 1986, 2862, 2952, 4677
BsmI	GAATGC	5 cut(s)	1021, 1491, 2608, 3968, 4559
BsnI	GGCC	8 cut(s)	137, 1571, 2789, 3808, 4012, 4315, 4434, 4652
Bso31I	GGTCTC	5 cut(s)	44, 207, 1549, 2463, 4569
BsoBI	CYCGRG	1 cut(s)	3521
Bsp119I	TTCGAA	2 cut(s)	2072, 4262
Bsp1286I	GDGCHC	5 cut(s)	572, 1471, 2519, 4108, 4257
Bsp1407I	TGTACA	1 cut(s)	1314
Bsp19I	CCATGG	5 cut(s)	1795, 1968, 2434, 2733, 4388
BspANI	GGCC	8 cut(s)	137, 1571, 2789, 3808, 4012, 4315, 4434, 4652
BspFNI	CGCG	3 cut(s)	14, 364, 670
BspMAI	CTGCAG	3 cut(s)	790, 1414, 4099
BspMI	ACCTGC	4 cut(s)	151, 631, 1207, 4088
BspOI	GCTAGC	1 cut(s)	2616
BspPI	GGATC	8 cut(s)	306, 318, 1757, 1951, 2371, 3227, 4378, 4478
BspQI	GCTCTTC	3 cut(s)	107, 183, 3480
BspT104I	TTCGAA	2 cut(s)	2072, 4262
BspT107I	GGYRCC	5 cut(s)	59, 968, 1893, 2750, 4392
BspTI	CTTAAG	1 cut(s)	3559
BspTNI	GGTCTC	5 cut(s)	44, 207, 1549, 2463, 4569
BsrDI	GCAATG	5 cut(s)	744, 2004, 3190, 3556, 4337
BsrFI	RCCGGY	1 cut(s)	146
BsrGI	TGTACA	1 cut(s)	1314
BsrI	ACTGG	9 cut(s)	1100, 2307, 2827, 3008, 3176, 3534, 4369, 4457, 4588
BssAI	RCCGGY	1 cut(s)	146
BssHII	GCGCGC	1 cut(s)	362
BssNAI	GTATAC	1 cut(s)	2833
BssT1I	CCWWGG	8 cut(s)	1795, 1958, 1968, 2434, 2733, 2790, 4173, 4388
Bst1107I	GTATAC	1 cut(s)	2833
Bst2UI	CCWGG	7 cut(s)	199, 1573, 3003, 3020, 3908, 4317, 4529
BstAFI	CTTAAG	1 cut(s)	3559
BstAPI	GCANNNNNTGC	1 cut(s)	1475
BstAUI	TGTACA	1 cut(s)	1314
BstBI	TTCGAA	2 cut(s)	2072, 4262
BstDSI	CCRYGG	7 cut(s)	12, 1795, 1968, 2434, 2576, 2733, 4388
BstEII	GGTNACC	2 cut(s)	411, 3902
BstENI	CCTNNNNNAGG	1 cut(s)	2700
BstFNI	CGCG	3 cut(s)	14, 364, 670
BstH2I	RGCGCY	2 cut(s)	4323, 4417
BstHHI	GCGC	8 cut(s)	364, 366, 670, 1840, 3988, 4322, 4416, 4559
BstMAI	GTCTC	8 cut(s)	44, 207, 998, 1549, 2463, 2540, 4125, 4569
BstNI	CCWGG	7 cut(s)	199, 1573, 3003, 3020, 3908, 4317, 4529
BstNSI	RCATGY	4 cut(s)	234, 451, 1297, 4028
BstPI	GGTNACC	2 cut(s)	411, 3902
BstSFI	CTRYAG	6 cut(s)	634, 786, 1410, 3100, 4095, 4155
BstSLI	GKGCMC	1 cut(s)	2519
BstUI	CGCG	3 cut(s)	14, 364, 670
BstV1I	GCAGC	9 cut(s)	29, 38, 47, 211, 214, 800, 3604, 3970, 3992
BstV2I	GAAGAC	2 cut(s)	2303, 4328
BstX2I	RGATCY	3 cut(s)	4050, 4144, 4483
BstXI	CCANNNNNNTGG	1 cut(s)	2740
BstYI	RGATCY	3 cut(s)	4050, 4144, 4483
BstZ17I	GTATAC	1 cut(s)	2833
Bsu36I	CCTNAGG	2 cut(s)	94, 2697
BsuI	GTATCC	1 cut(s)	3580
BsuRI	GGCC	8 cut(s)	137, 1571, 2789, 3808, 4012, 4315, 4434, 4652
BtgI	CCRYGG	7 cut(s)	12, 1795, 1968, 2434, 2576, 2733, 4388
BtsI	GCAGTG	3 cut(s)	2478, 3665, 4689
BveI	ACCTGC	4 cut(s)	151, 631, 1207, 4088
CaiI	CAGNNNCTG	3 cut(s)	927, 2034, 4322
CfoI	GCGC	8 cut(s)	364, 366, 670, 1840, 3988, 4322, 4416, 4559
Cfr10I	RCCGGY	1 cut(s)	146
Cfr13I	GGNCC	7 cut(s)	349, 1460, 1738, 1973, 3005, 3698, 4432
Cfr42I	CCGCGG	1 cut(s)	15
CseI	GACGC	1 cut(s)	3431
CsiI	ACCWGGT	1 cut(s)	3906
DraI	TTTAAA	2 cut(s)	2052, 2803
DraIII	CACNNNGTG	1 cut(s)	3121
DrdI	GACNNNNNNGTC	1 cut(s)	3705
DriI	GACNNNNNGTC	1 cut(s)	3870
DseDI	GACNNNNNNGTC	1 cut(s)	3705
EaeI	YGGCCR	3 cut(s)	3806, 4010, 4313
Eam1105I	GACNNNNNGTC	1 cut(s)	3870
Eco130I	CCWWGG	8 cut(s)	1795, 1958, 1968, 2434, 2733, 2790, 4173, 4388
Eco24I	GRGCYC	2 cut(s)	572, 4108
Eco31I	GGTCTC	5 cut(s)	44, 207, 1549, 2463, 4569
Eco32I	GATATC	2 cut(s)	1264, 2563
Eco47I	GGWCC	6 cut(s)	349, 1460, 1738, 1973, 3005, 3698
Eco57I	CTGAAG	3 cut(s)	207, 2802, 3453
Eco81I	CCTNAGG	2 cut(s)	94, 2697
Eco88I	CYCGRG	1 cut(s)	3521
Eco91I	GGTNACC	2 cut(s)	411, 3902
EcoNI	CCTNNNNNAGG	1 cut(s)	2700
EcoO109I	RGGNCCY	3 cut(s)	1973, 3698, 4432
EcoO65I	GGTNACC	2 cut(s)	411, 3902
EcoRI	GAATTC	1 cut(s)	2809
EcoRII	CCWGG	7 cut(s)	197, 1571, 3001, 3018, 3906, 4315, 4527
EcoRV	GATATC	2 cut(s)	1264, 2563
EcoT14I	CCWWGG	8 cut(s)	1795, 1958, 1968, 2434, 2733, 2790, 4173, 4388
EcoT22I	ATGCAT	2 cut(s)	1021, 4026
EcoT38I	GRGCYC	2 cut(s)	572, 4108
ErhI	CCWWGG	8 cut(s)	1795, 1958, 1968, 2434, 2733, 2790, 4173, 4388
FaqI	GGGAC	6 cut(s)	560, 1446, 1986, 2862, 2952, 4677
FauI	CCCGC	3 cut(s)	213, 1995, 3575
FauNDI	CATATG	1 cut(s)	3974
FbaI	TGATCA	4 cut(s)	649, 832, 871, 2022
FblI	GTMKAC	2 cut(s)	2832, 3096
FriOI	GRGCYC	2 cut(s)	572, 4108
FspBI	CTAG	4 cut(s)	2613, 3312, 3392, 3926
FspI	TGCGCA	1 cut(s)	1839
GlaI	GCGC	8 cut(s)	363, 365, 669, 1839, 3987, 4321, 4415, 4558
GsaI	CCCAGC	2 cut(s)	421, 4154
GsuI	CTGGAG	4 cut(s)	2046, 2990, 3954, 4205
HaeII	RGCGCY	2 cut(s)	4323, 4417
HaeIII	GGCC	8 cut(s)	137, 1571, 2789, 3808, 4012, 4315, 4434, 4652
HapII	CCGG	7 cut(s)	147, 1023, 1464, 3414, 3671, 3805, 4040
HgaI	GACGC	1 cut(s)	3431
HhaI	GCGC	8 cut(s)	364, 366, 670, 1840, 3988, 4322, 4416, 4559
Hin6I	GCGC	8 cut(s)	362, 364, 668, 1838, 3986, 4320, 4414, 4557
HinP1I	GCGC	8 cut(s)	362, 364, 668, 1838, 3986, 4320, 4414, 4557
HincII	GTYRAC	1 cut(s)	4378
HindII	GTYRAC	1 cut(s)	4378
HindIII	AAGCTT	3 cut(s)	1925, 2683, 2946
HpaII	CCGG	7 cut(s)	147, 1023, 1464, 3414, 3671, 3805, 4040
HphI	GGTGA	6 cut(s)	405, 2297, 3311, 3509, 3922, 4363
Hpy99I	CGWCG	1 cut(s)	106
HpyCH4IV	ACGT	3 cut(s)	1120, 2276, 3388
HpySE526I	ACGT	3 cut(s)	1120, 2276, 3388
HspAI	GCGC	8 cut(s)	362, 364, 668, 1838, 3986, 4320, 4414, 4557
KpnI	GGTACC	1 cut(s)	972
KroI	GCCGGC	1 cut(s)	146
KroNI	GCCGGC	1 cut(s)	148
Ksp22I	TGATCA	4 cut(s)	649, 832, 871, 2022
KspI	CCGCGG	1 cut(s)	15
LguI	GCTCTTC	3 cut(s)	107, 183, 3480
LmnI	GCTCC	9 cut(s)	1466, 1532, 2234, 2449, 2840, 3193, 3935, 4111, 4166
Lsp1109I	GCAGC	9 cut(s)	29, 38, 47, 211, 214, 800, 3604, 3970, 3992
MabI	ACCWGGT	1 cut(s)	3906
MaeI	CTAG	4 cut(s)	2613, 3312, 3392, 3926
MaeII	ACGT	3 cut(s)	1120, 2276, 3388
MaeIII	GTNAC	7 cut(s)	411, 1381, 3121, 3193, 3525, 3902, 3954
MfeI	CAATTG	2 cut(s)	3474, 4137
MflI	RGATCY	3 cut(s)	4050, 4144, 4483
MhlI	GDGCHC	5 cut(s)	572, 1471, 2519, 4108, 4257
MlsI	TGGCCA	1 cut(s)	4012
MluNI	TGGCCA	1 cut(s)	4012
MlyI	GAGTC	4 cut(s)	1318, 1319, 2270, 3533
MmeI	TCCRAC	4 cut(s)	414, 1323, 2201, 2256
Mox20I	TGGCCA	1 cut(s)	4012
Mph1103I	ATGCAT	2 cut(s)	1021, 4026
MroNI	GCCGGC	1 cut(s)	146
MroXI	GAANNNNTTC	3 cut(s)	1350, 1623, 3731
MscI	TGGCCA	1 cut(s)	4012
MslI	CAYNNNNRTG	3 cut(s)	2319, 2862, 3973
Msp20I	TGGCCA	1 cut(s)	4012
MspA1I	CMGCKG	6 cut(s)	14, 20, 29, 38, 3983, 4154
MspCI	CTTAAG	1 cut(s)	3559
MspI	CCGG	7 cut(s)	147, 1023, 1464, 3414, 3671, 3805, 4040
MunI	CAATTG	2 cut(s)	3474, 4137
Mva1269I	GAATGC	5 cut(s)	1021, 1491, 2608, 3968, 4559
MvaI	CCWGG	7 cut(s)	199, 1573, 3003, 3020, 3908, 4317, 4529
MvnI	CGCG	3 cut(s)	14, 364, 670
NaeI	GCCGGC	1 cut(s)	148
NciI	CCSGG	3 cut(s)	1464, 3415, 3672
NcoI	CCATGG	5 cut(s)	1795, 1968, 2434, 2733, 4388
NdeI	CATATG	1 cut(s)	3974
NgoMIV	GCCGGC	1 cut(s)	146
NheI	GCTAGC	1 cut(s)	2612
NmeAIII	GCCGAG	2 cut(s)	65, 2869
NmuCI	GTSAC	5 cut(s)	411, 3121, 3193, 3525, 3954
NsbI	TGCGCA	1 cut(s)	1839
NsiI	ATGCAT	2 cut(s)	1021, 4026
NspI	RCATGY	4 cut(s)	234, 451, 1297, 4028
NspV	TTCGAA	2 cut(s)	2072, 4262
PaeI	GCATGC	1 cut(s)	1297
PalAI	GGCGCGCC	1 cut(s)	362
PaqCI	CACCTGC	2 cut(s)	151, 4088
PauI	GCGCGC	1 cut(s)	362
PciI	ACATGT	1 cut(s)	447
PciSI	GCTCTTC	3 cut(s)	107, 183, 3480
PcsI	WCGNNNNNNNCGW	2 cut(s)	1687, 2990
PctI	GAATGC	5 cut(s)	1021, 1491, 2608, 3968, 4559
PdiI	GCCGGC	1 cut(s)	148
PdmI	GAANNNNTTC	3 cut(s)	1350, 1623, 3731
PflMI	CCANNNNNTGG	2 cut(s)	2968, 4209
PfoI	TCCNGGA	1 cut(s)	1462
PleI	GAGTC	4 cut(s)	1317, 1319, 2269, 3532
PpsI	GAGTC	4 cut(s)	1317, 1319, 2269, 3532
PpuMI	RGGWCCY	2 cut(s)	1973, 3698
PscI	ACATGT	1 cut(s)	447
PshBI	ATTAAT	1 cut(s)	2570
PsiI	TTATAA	1 cut(s)	2298
Psp5II	RGGWCCY	2 cut(s)	1973, 3698
Psp6I	CCWGG	7 cut(s)	197, 1571, 3001, 3018, 3906, 4315, 4527
PspEI	GGTNACC	2 cut(s)	411, 3902
PspFI	CCCAGC	2 cut(s)	417, 4150
PspGI	CCWGG	7 cut(s)	197, 1571, 3001, 3018, 3906, 4315, 4527
PspPI	GGNCC	7 cut(s)	349, 1460, 1738, 1973, 3005, 3698, 4432
PspPPI	RGGWCCY	2 cut(s)	1973, 3698
PsrI	GAACNNNNNNTAC	2 cut(s)	4058, 4090
PstI	CTGCAG	3 cut(s)	790, 1414, 4099
PstNI	CAGNNNCTG	3 cut(s)	927, 2034, 4322
PsuI	RGATCY	3 cut(s)	4050, 4144, 4483
PteI	GCGCGC	1 cut(s)	362
PvuII	CAGCTG	3 cut(s)	20, 3983, 4154
RseI	CAYNNNNRTG	3 cut(s)	2319, 2862, 3973
SacII	CCGCGG	1 cut(s)	15
SapI	GCTCTTC	3 cut(s)	107, 183, 3480
Sau96I	GGNCC	7 cut(s)	349, 1460, 1738, 1973, 3005, 3698, 4432
ScaI	AGTACT	4 cut(s)	1094, 2045, 2849, 4093
SchI	GAGTC	4 cut(s)	1318, 1319, 2270, 3533
SduI	GDGCHC	5 cut(s)	572, 1471, 2519, 4108, 4257
SexAI	ACCWGGT	1 cut(s)	3906
SfcI	CTRYAG	6 cut(s)	634, 786, 1410, 3100, 4095, 4155
Sfr303I	CCGCGG	1 cut(s)	15
SfuI	TTCGAA	2 cut(s)	2072, 4262
SgrBI	CCGCGG	1 cut(s)	15
SgsI	GGCGCGCC	1 cut(s)	362
SinI	GGWCC	6 cut(s)	349, 1460, 1738, 1973, 3005, 3698
SmiI	ATTTAAAT	1 cut(s)	2052
SmiMI	CAYNNNNRTG	3 cut(s)	2319, 2862, 3973
SmlI	CTYRAG	7 cut(s)	813, 1327, 1498, 3559, 3893, 4566, 4673
SmoI	CTYRAG	7 cut(s)	813, 1327, 1498, 3559, 3893, 4566, 4673
SphI	GCATGC	1 cut(s)	1297
SspI	AATATT	1 cut(s)	2672
SspMI	CTAG	4 cut(s)	2613, 3312, 3392, 3926
StyI	CCWWGG	8 cut(s)	1795, 1958, 1968, 2434, 2733, 2790, 4173, 4388
SwaI	ATTTAAAT	1 cut(s)	2052
TaiI	ACGT	3 cut(s)	1123, 2279, 3391
TaqII	GACCGA	1 cut(s)	4551
TatI	WGTACW	5 cut(s)	1092, 1314, 2043, 2847, 4091
TauI	GCSGC	7 cut(s)	14, 17, 41, 140, 362, 1428, 4315
TseFI	GTSAC	5 cut(s)	411, 3121, 3193, 3525, 3954
TseI	GCWGC	9 cut(s)	17, 26, 35, 224, 227, 788, 3617, 3980, 3983
Tsp45I	GTSAC	5 cut(s)	411, 3121, 3193, 3525, 3954
TspGWI	ACGGA	5 cut(s)	2195, 2909, 3693, 3860, 4103
Van91I	CCANNNNNTGG	2 cut(s)	2968, 4209
Vha464I	CTTAAG	1 cut(s)	3559
VneI	GTGCAC	1 cut(s)	2515
VpaK11BI	GGWCC	6 cut(s)	349, 1460, 1738, 1973, 3005, 3698
VspI	ATTAAT	1 cut(s)	2570
XagI	CCTNNNNNAGG	1 cut(s)	2700
XapI	RAATTY	6 cut(s)	770, 800, 1826, 2325, 2809, 2932
XbaI	TCTAGA	1 cut(s)	3925
XceI	RCATGY	4 cut(s)	234, 451, 1297, 4028
XcmI	CCANNNNNNNNNTGG	3 cut(s)	1975, 4180, 4323
XmiI	GTMKAC	2 cut(s)	2832, 3096
XmnI	GAANNNNTTC	3 cut(s)	1350, 1623, 3731
XspI	CTAG	4 cut(s)	2613, 3312, 3392, 3926
ZrmI	AGTACT	4 cut(s)	1094, 2045, 2849, 4093
Zsp2I	ATGCAT	2 cut(s)	1021, 4026

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.