Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0002932

Physical Location & Seq

Genomic Coordinates Forward (+)

18915726 .. 18921878

Gene Structure 6153 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1231100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4710 bp

ATGGGTTCCGCCGCGGAAGCAGCGGCGGTCTTGGAGACCAAAGGTGCCAATGGGGCTAAGCCACCGGCTTCTCCTTCCTCAGGAATGACGAAGAAGAAGAAGAAGGGCAAACCAGCTTCCCAAAAGGCAGCACCTACTGGCTCCAAAACTAAGAAGCGAAATTTGCCTCAGAGCACTGAAGAGCCTTCCAAGTCTCGGAAAATGCCCAAGCGGGCTGCTGCATGCACAGACTTTAAAGAGAAGTCTGTTCACATTTCTGAAAAATCTAATCTTATTGAAAGCAAGAAGGATCGAGTAGTTGATCAAGAAACTGATGCTATAGTCCTGACTTCTGGCGAGGACCAAGATGCAGCACGCCCAAACAGACGGCTGACTGATTTTATTGTGCATGATGAAAGTGGGTTACCCCAGCCTGTTGAGATGTTGGAAGTTGCTGACATGTTTATATCTGGTACTATATTGCCTCTCAATGAAAGTTCTGACAAGGACAAGGAAAGAGGTGTTAGATGTGAAGGTTTTGGGCGAATAGAATCTTGGAACATCTCTGGTTATGAAGAGGGCTCTCCTGTGATATGGCTCTCAACTGATGTTGCTGATTATGATTGTCGTAAACCTGCCAGCTTCTATAGGAAATACCATGATCAATTCTTTGAGAAAGCGCGTGCTTGCATAGAGGTTTACAAAAAGCTGTCAAAATCCAAATCTGACACTACTCTTGATGAATTACTTGCTGCCATTGCTCGATCAATGAGTGGAACCAAATTCTTTTCCGGTACTGCAGCTATCAAAGAATTTGTTATATCTCAAGGTGAGTTTATCTATGATCAACTGGTAGGTTTGGAGGAAACATCAAAGAAGAATGATCTGCCGTTTACAGAGCTGCCTGTTCTTGCTGCCCTTAGAGATGAGAGCAGAAAGTGTGGAAGTTTTGTGCAATCAAAACCGGCAACTTCAAGTGGAACTCTAAAGATTGGTTCGGAAGTTATAGGCGGAGAGGATGGACTGATTTCAGCCGGTTCATCCATAGTTGAAGCTGAGGAAAATGAGGATGTAAAGTTGGCAAGACTTTTGCAAGAAGAAGAGTACTGGAAGTCAATGAAACAGAAAAAGCGTCAGGGTTCTGCCCCTGGGTCGAGCAAATACTACATCAAAATTAATGAAGATGAGATTGCCAATGATTATCCTCTACCTGCTTATTACAAGAATTCCATTGAAGAAACTGACGAGTTCATAGTTTTTGACAATGACTATGATATCTTAAATGTTGATGACCTTCCTCGAAGCATGCTTCATAATTGGTGTCTGTACAATTCAGACTCAAGATTGATTTCGTTGGAACTTCTTCCAATGAAACCTTGTGCAGACATTGATGTTACCATTTTTGGGTCGGGGGTTATGACTGGAGATGATGGAAGCGGCTTTTTTCTTGATTCTGATGGTTCTTCAAGTGGTCCAGGAGCAAAGGATGCTGATGGAATGCCGATTTACTTGAGTGCAATAAAGGAGTGGATGATTGAATTGGGAGCATCAATGATCTCAATATCAATCCGAACAGATTTGGCCTGGTATAGACTTGGACAGCCATCTAAGCAGTATGCTCTGTGGTATGAACCAATTCTAAAAACAGCAAAGGTTGGTAGAAGCATAATCACTTTGCTGAAAGAGCAAAGTCGAGTAGCACGGCTTTCTTTTGCAGAGGTCATTAAGAGATTGTCAGGGTTTCCAAAGGACCACTGTGCGTACATTTCTTCTGATCCGGCATTTGTTGAGAGATATGTGGTTGTCCATGGACAGATAATACTGCAACTGTTTTCAGAATTTCCTGATGCGCAGATTAAAAAGTGCCCATTTGTGGTTGGACTTACAAAGAAAATGGAGGAGAGACACCATACTAAGTGGTTGGTAAAGAAGAAGAAGGTTGTGGAGAAGAGTGGATCAAACTTGAACCCGAGGGCATCCATGGGACCTGTGGTTTCCAAGAAGAAGGCAATGCGGGCTACAACAACAAGGCTGATCAACAGAATCTGGGGGGAGTACTATTTAAATTACTCTCCAGAAGATTCGAATGAGGAGGAAGCTAATGGTGAAAAGAAAGAGGAGGAAGTTGAAGAAGAGGAGGATAAGGAGGACATAGAAGAGGATGATGATGAAGAGAACGATAATGCAGCAGTAACGGAACAAGCCCAGAAGCCTAGTTCAATTTCGAGACAAACTAAATCATGCTCCAACAACAAGGAGATTCTGTGGGATGGGGAGCCTGTAGGCACAACATGTTCTGGCGAAGCTCTTTATATGCGTGCCAGTCTTCGTGGAGATGAAATTTCTGTTGGTGGTGCAGTCTTGGTAGAACTTGATGGATCAGATGAACTTCCTGCCATTTATTATGTGGAGTATATGTATGAAACAAGAAATGGAAGCAAAATGTTTCATGGGAGATTGATGGAACGAGGATCTCAGACTGTTCTTGGCAACACTGCCAACGAGAGGGAGGTATTTTTGACAAATGAGTGCACAAACTTGGCATTAAAGGTTGTTAAAGAGACATCAGTTGTGGATATCAAATTATTGCCGTGGGGGCATCAGTACAGGAAGGAGAATGAGGAAGCTAGCCAAAAAGACAGAGAAAGGGCAGAAGATAGGAAGAAGAAGGGATTGCCAACTGAATATTACTGTAAAAGCTTGTATTGCCCCGGGGAAGGTGCATTCTTTAGCCTTTCCTGTGATACCATGGGTCTGGGTTCTGGTGCTTGCAACTCTTGCAAAGTGAACGAAGCTGAGGAGGCCAAGGAGATTTTTAAAGTGAATTCATCAAAGACTGGTTTTGTATACAGGGGAGCCGAGTACTCAGTTCATGATTATGTCTATTTAAGTCCCCATTTGTTTTCTACTGAAAGGATGGAAACTGAAACTTTCAAGGCTGGTAGGAATTTGGGTCTGAGAGCTTATGTTGTGTGTCAAGTGCTGGAGATAATTGGTACGAAGGAATCTAAACGACCTGGACCAGAATCTACCCAGGTTAAAGTGAGAAGATTTTTCAGACCAGAGGACATTTCGGTTGAGAAGGCATACTGTTCTGATATTAGAGAGGTCTACTACAGTGAAGAAACACACCTTGTGACTGTTGATGATATAGAAGGAAAATGTGAAGTCAGAAAGAAGAGTGATCTTCCAGTATGCAATGCTCCTGTCACTTTTGAGCATATCTTCTTCTGTGAGTATCTGTATGATCCTTCTAATGGGTCTATCAAGCAGTTGCCGGCGGCCACCAAACTGAGGTACTCAACAGGAGGAGGTGGTGCGGAATCTAGGAAGAGGAAGGGGAAAATCAAAGAAGGAGAAGATGTTTCAGAAGTTGAGAAACAGAGAGTTGATGCTGAGCAGAAACGTCTAGCCACATTAGATATATTTGCCGGGTGTGGTGGCTTGTCTGAAGGGCTGCGTCAGGCTGGCATTTCATTAACCAAGTGGGCAATTGAGTACGAAGAGCCTGCTGGTGATGCTTTCAAACTGAACCATCCCGAGTCACTGGTGTTTATCAATAACTGCAATGTGATCTTAAGGGCTGTAATGGAGAAATGTGGGGATACAGATGATTGCATCTCAACTTCTGAAGCTGCTGAATTGGCTAAAGCACTCGATGAGAAGGTTAAGAATGATCTGCCACTGCCATGGCAAGTGGATTTCATCAATGGAGGACCTCCATGCCAGGGTTTCTCTGGAATGAATAGGTTCAACCAAAGCACTTGGAGTAAAGTTCAGTGTGAAATGATTTTGGCATTCTTGTCCTTTGCTGACTACTTCCGGCCAAAGTATTTCCTCCTGGAGAATGTGAGGAACCTTGTGTCTTTCAACAAAGGACAGACTTTCCGTCTGACTCTGGCTTCCCTTCTTGAGATGGGTTACCAGGTGAGGTTTGGTATTCTGGAAGCTGGAGCTTATGGAGTTTCCCAGTCACGGAAGCGAGCATTCATATGGGCAGCTGCGCCCGATGAGATTCTCCCGGAGTGGCCAGAGCCAATGCATGTCTTTGGTGTACCAGAGTTGAAGATCAATTTGTCAGGGAACTCATATTATGCTGCTGTTCGAAGTACTGCAGGGGGAGCCCCTTTCCGTTCCATAACTGTCAGAGATACAATTGGAGATCTCCCAGCTGTAGGAAATGGAGCTTCTAAGGTCAATCTGGAGTACGAAGGTGACCCCATCTCATGGTTCCAGAAGAAAATGCGAGGGGAGATGACTGTTCTGACTGATCACATTTCGAAAGAAATGAATGAGCTGAATCTCATTCGCTGCCAGAGGATTCCAAAGCGGCCAGGTGCTGATTGGCAATGCCTTCCAGACGAAAAGGTGAAGCTCTCTACTGGACAGATTGTTGACTTAATCCCGTGGTGCCTGCCCAACACGGCCAAGCGCCACAATCAGTGGAAGGGCCTGTTCGGAAGGCTTGACTGGGAAGGCAACTTCCCAACCTCCATCACAGATCCCCAGCCAATGGGAAAGGTGGGAATGTGCTTCCACCCTGACCAGGACAGAATTCTGACAGTTCGGGAATGCGCTCGGTCTCAAGGATTCAGGGATAGCTACCAGTTTTCGGGCAACATTCTTCACAAGCACAGGCAGATTGGAAATGCAGTTCCTCCTACTTTGGCCTATGCGTTGGGACGAAAACTCAAGGAAGCAGTGGACAGCAAGAACAAGGCAGAGGAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000278 GO:0000281 GO:0000910 GO:0003006 GO:0003674 GO:0003824 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006349 GO:0006725 GO:0006807 GO:0007049 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008356 GO:0009292 GO:0009294 GO:0009790 GO:0009791 GO:0009793 GO:0009909 GO:0009910 GO:0009987 GO:0010069 GO:0010070 GO:0010154 GO:0010216 GO:0010424 GO:0010468 GO:0016740 GO:0016741 GO:0019222 GO:0022402 GO:0022414 GO:0032259 GO:0032501 GO:0032502 GO:0032776 GO:0034641 GO:0040029 GO:0043170 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044728 GO:0044764 GO:0046483 GO:0048316 GO:0048519 GO:0048580 GO:0048581 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0051093 GO:0051239 GO:0051241 GO:0051301 GO:0051704 GO:0060255 GO:0061458 GO:0061640 GO:0065007 GO:0071514 GO:0071704 GO:0090304 GO:1901360 GO:1903047 GO:2000026 GO:2000241 GO:2000242

KEGG Pathways

Metabolic & Signaling

ko00270 ko01100 ko05206 map00270 map01100 map05206

Pfam Domains

Protein Families

BAH DNA_methylase DNMT1-RFD

Protein Analysis

1570

Amino Acids

175.32

Weight (kDa)

5.65

Isoelectric Point (pI)

45.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DNMT1-RFD	PF12047	118 - 248	9.9e-37	Cytosine specific DNA methyltransferase replication foci domain
DNMT1-RFD	PF12047	423 - 573	6e-35	Cytosine specific DNA methyltransferase replication foci domain
BAH	PF01426	773 - 905	4e-15	BAH domain
BAH	PF01426	947 - 1086	6.5e-24	BAH domain
DNA_methylase	PF00145	1219 - 1558	9.6e-41	C-5 cytosine-specific DNA methylase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0002932)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G08990 AT4G14140 AT4G14140 AT4G14140 AT5G49160 AT5G49160
fragaria_vesca	FvH4_1g10300 FvH4_1g10300
malus_domestica	MD02G1115100.v1.1 MD15G1231100.v1.1
prunus_persica	Prupe.7G060100_v2.0.a1 Prupe.7G183100_v2.0.a1 Prupe.7G183100_v2.0.a1
pyrus_communis	pycom02g09000 pycom02g09010 pycom15g20590 pycom15g20600
rosa_chinensis	RchiOBHm_Chr2g0097141 RchiOBHm_Chr5g0051581
rosa_laevigata	RLG00000016706 RLG00000034873
rosa_multiflora	Rmu_co8502141.1_g000001 Rmu_sc0001182.1_g000006 Rmu_ssc0000089.1_g000039
rosa_roxburghii	Rroxscaffold_1G00027090 Rroxscaffold_2G00144940
rosa_rugosa	Rorug02G0064600 Rorug05G0279000
rosa_samantha	Rh2AG111700 Rh2BG113700 Rh2CG116000 Rh2DG115000 Rh5AG350100 Rh5BG361900 Rh5CG386800 Rh5DG376800
rosa_wichuraiana	Rw2G008690 Rw5G033130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1830
Acc36I	ACCTGC	2 cut(s)	622, 1198
AccB1I	GGYRCC	2 cut(s)	44, 4389
AccB7I	CCANNNNNTGG	2 cut(s)	4206, 4492
AccI	GTMKAC	2 cut(s)	2826, 3090
AccII	CGCG	2 cut(s)	14, 661
AclWI	GGATC	7 cut(s)	297, 1748, 1942, 2365, 2458, 3221, 4475
AcoI	YGGCCR	5 cut(s)	3261, 3803, 4007, 4310, 4404
AcsI	RAATTY	9 cut(s)	160, 761, 791, 1204, 1817, 2319, 2803, 2926, 4533
AcuI	CTGAAG	3 cut(s)	198, 3450, 3630
AdeI	CACNNNGTG	1 cut(s)	3115
AflII	CTTAAG	1 cut(s)	3556
AflIII	ACRYGT	2 cut(s)	438, 2270
AhdI	GACNNNNNGTC	1 cut(s)	3867
AjuI	GAANNNNNNNTTGG	4 cut(s)	517, 549, 2310, 2342
AloI	GAACNNNNNNTCC	2 cut(s)	4617, 4649
Alw21I	GWGCWC	2 cut(s)	176, 2513
Alw26I	GTCTC	6 cut(s)	29, 198, 1876, 2200, 2534, 4566
Alw44I	GTGCAC	1 cut(s)	2509
AlwI	GGATC	7 cut(s)	297, 1748, 1942, 2365, 2458, 3221, 4475
AlwNI	CAGNNNCTG	2 cut(s)	2025, 4319
Ama87I	CYCGRG	3 cut(s)	1948, 2690, 3518
AoxI	GGCC	9 cut(s)	1560, 2781, 3261, 3803, 4007, 4310, 4404, 4429, 4647
ApaLI	GTGCAC	1 cut(s)	2509
ApoI	RAATTY	9 cut(s)	160, 761, 791, 1204, 1817, 2319, 2803, 2926, 4533
AseI	ATTAAT	1 cut(s)	1155
Asp700I	GAANNNNTTC	3 cut(s)	1341, 1614, 3728
AspLEI	GCGC	5 cut(s)	661, 1831, 3985, 4413, 4556
AspS9I	GGNCC	7 cut(s)	340, 1451, 1729, 1964, 2999, 3695, 4429
AsuC2I	CCSGG	4 cut(s)	2691, 2692, 3412, 4001
AsuHPI	GGTGA	6 cut(s)	821, 2096, 3506, 3919, 4205, 4360
AsuII	TTCGAA	3 cut(s)	2063, 4084, 4259
AsuNHI	GCTAGC	1 cut(s)	2606
AvaI	CYCGRG	3 cut(s)	1948, 2690, 3518
AvaII	GGWCC	6 cut(s)	340, 1451, 1729, 1964, 2999, 3695
AxyI	CCTNAGG	1 cut(s)	79
BaeGI	GKGCMC	2 cut(s)	1847, 2513
BalI	TGGCCA	1 cut(s)	4009
BanI	GGYRCC	2 cut(s)	44, 4389
BanII	GRGCYC	2 cut(s)	563, 4105
BbsI	GAAGAC	1 cut(s)	2297
Bbv12I	GWGCWC	2 cut(s)	176, 2513
BbvCI	CCTCAGC	2 cut(s)	1035, 2775
BceAI	ACGGC	5 cut(s)	383, 853, 1697, 2554, 4419
BcgI	CGANNNNNNTGC	2 cut(s)	3470, 3504
BciVI	GTATCC	1 cut(s)	3577
BclI	TGATCA	5 cut(s)	301, 640, 823, 2013, 4249
BcnI	CCSGG	4 cut(s)	2691, 2692, 3412, 4001
BcoDI	GTCTC	6 cut(s)	29, 198, 1876, 2200, 2534, 4566
BfaI	CTAG	4 cut(s)	2193, 2607, 3306, 3389
BfmI	CTRYAG	7 cut(s)	318, 625, 777, 2259, 3094, 4092, 4152
BfoI	RGCGCY	1 cut(s)	4414
BfrI	CTTAAG	1 cut(s)	3556
BfuAI	ACCTGC	2 cut(s)	622, 1198
BfuI	GTATCC	1 cut(s)	3577
BglI	GCCNNNNNGGC	2 cut(s)	53, 2575
BglII	AGATCT	1 cut(s)	4141
BlpI	GCTNAGC	2 cut(s)	57, 3375
BmcAI	AGTACT	4 cut(s)	1085, 2036, 2843, 4090
Bme18I	GGWCC	6 cut(s)	340, 1451, 1729, 1964, 2999, 3695
BmeRI	GACNNNNNGTC	1 cut(s)	3867
BmeT110I	CYCGRG	3 cut(s)	1948, 2690, 3518
BmgT120I	GGNCC	7 cut(s)	340, 1451, 1729, 1964, 2999, 3695, 4429
BmrI	ACTGGG	2 cut(s)	3943, 4459
BmtI	GCTAGC	1 cut(s)	2610
BmuI	ACTGGG	2 cut(s)	3943, 4459
BpiI	GAAGAC	1 cut(s)	2297
BpmI	CTGGAG	6 cut(s)	1422, 2037, 2984, 3842, 3951, 4202
Bpu10I	CCTNAGC	2 cut(s)	1035, 2775
Bpu1102I	GCTNAGC	2 cut(s)	57, 3375
Bpu14I	TTCGAA	3 cut(s)	2063, 4084, 4259
BpuEI	CTTGAG	6 cut(s)	789, 1303, 1510, 3911, 4548, 4655
BpuMI	CCSGG	4 cut(s)	2691, 2692, 3412, 4001
BsaI	GGTCTC	2 cut(s)	29, 4566
BsaWI	WCCGGW	1 cut(s)	770
BsaXI	ACNNNNNCTCC	6 cut(s)	2228, 2258, 2381, 2411, 3327, 3357
Bse118I	RCCGGY	4 cut(s)	64, 943, 1013, 3256
Bse21I	CCTNAGG	1 cut(s)	79
Bse3DI	GCAATG	5 cut(s)	735, 1995, 3184, 3553, 4334
BseMI	GCAATG	5 cut(s)	735, 1995, 3184, 3553, 4334
BseMII	CTCAG	9 cut(s)	93, 182, 1026, 2468, 2766, 2859, 2927, 3263, 3366
BseRI	GAGGAG	6 cut(s)	1892, 2084, 2111, 2129, 2792, 3303
BseSI	GKGCMC	2 cut(s)	1847, 2513
BseYI	CCCAGC	3 cut(s)	408, 4147, 4485
BsgI	GTGCAG	2 cut(s)	1380, 2355
Bsh1236I	CGCG	2 cut(s)	14, 661
BshFI	GGCC	9 cut(s)	1562, 2783, 3263, 3805, 4009, 4312, 4406, 4431, 4649
BshNI	GGYRCC	2 cut(s)	44, 4389
BsiHKAI	GWGCWC	2 cut(s)	176, 2513
BsiHKCI	CYCGRG	3 cut(s)	1948, 2690, 3518
BslFI	GGGAC	3 cut(s)	1977, 2856, 4674
BsmAI	GTCTC	6 cut(s)	29, 198, 1876, 2200, 2534, 4566
BsmFI	GGGAC	3 cut(s)	1977, 2856, 4674
BsmI	GAATGC	5 cut(s)	1482, 2702, 3776, 3965, 4556
BsnI	GGCC	9 cut(s)	1562, 2783, 3263, 3805, 4009, 4312, 4406, 4431, 4649
Bso31I	GGTCTC	2 cut(s)	29, 4566
BsoBI	CYCGRG	3 cut(s)	1948, 2690, 3518
Bsp119I	TTCGAA	3 cut(s)	2063, 4084, 4259
Bsp1286I	GDGCHC	5 cut(s)	176, 563, 1847, 2513, 4105
Bsp1407I	TGTACA	1 cut(s)	1305
Bsp1720I	GCTNAGC	2 cut(s)	57, 3375
Bsp19I	CCATGG	4 cut(s)	1786, 1959, 2727, 3668
BspANI	GGCC	9 cut(s)	1562, 2783, 3263, 3805, 4009, 4312, 4406, 4431, 4649
BspCNI	CTCAG	9 cut(s)	92, 181, 1027, 2467, 2767, 2858, 2928, 3264, 3367
BspFNI	CGCG	2 cut(s)	14, 661
BspHI	TCATGA	1 cut(s)	2851
BspMAI	CTGCAG	2 cut(s)	781, 4096
BspMI	ACCTGC	2 cut(s)	622, 1198
BspOI	GCTAGC	1 cut(s)	2610
BspPI	GGATC	7 cut(s)	297, 1748, 1942, 2365, 2458, 3221, 4475
BspQI	GCTCTTC	2 cut(s)	174, 3477
BspT104I	TTCGAA	3 cut(s)	2063, 4084, 4259
BspT107I	GGYRCC	2 cut(s)	44, 4389
BspTI	CTTAAG	1 cut(s)	3556
BspTNI	GGTCTC	2 cut(s)	29, 4566
BsrDI	GCAATG	5 cut(s)	735, 1995, 3184, 3553, 4334
BsrFI	RCCGGY	4 cut(s)	64, 943, 1013, 3256
BsrGI	TGTACA	1 cut(s)	1305
BssAI	RCCGGY	4 cut(s)	64, 943, 1013, 3256
BssNAI	GTATAC	1 cut(s)	2827
BssT1I	CCWWGG	5 cut(s)	1786, 1959, 2727, 2784, 3668
Bst1107I	GTATAC	1 cut(s)	2827
BstAFI	CTTAAG	1 cut(s)	3556
BstAPI	GCANNNNNTGC	2 cut(s)	1466, 2757
BstAUI	TGTACA	1 cut(s)	1305
BstBI	TTCGAA	3 cut(s)	2063, 4084, 4259
BstDSI	CCRYGG	7 cut(s)	12, 1786, 1959, 2570, 2727, 3668, 4385
BstEII	GGTNACC	3 cut(s)	402, 3899, 4193
BstENI	CCTNNNNNAGG	1 cut(s)	78
BstFNI	CGCG	2 cut(s)	14, 661
BstH2I	RGCGCY	1 cut(s)	4414
BstHHI	GCGC	5 cut(s)	661, 1831, 3985, 4413, 4556
BstMAI	GTCTC	6 cut(s)	29, 198, 1876, 2200, 2534, 4566
BstNSI	RCATGY	5 cut(s)	225, 442, 1288, 2274, 4025
BstPI	GGTNACC	3 cut(s)	402, 3899, 4193
BstSFI	CTRYAG	7 cut(s)	318, 625, 777, 2259, 3094, 4092, 4152
BstSLI	GKGCMC	2 cut(s)	1847, 2513
BstUI	CGCG	2 cut(s)	14, 661
BstV2I	GAAGAC	1 cut(s)	2297
BstX2I	RGATCY	3 cut(s)	2450, 4141, 4480
BstXI	CCANNNNNNTGG	2 cut(s)	2734, 3669
BstYI	RGATCY	3 cut(s)	2450, 4141, 4480
BstZ17I	GTATAC	1 cut(s)	2827
Bsu36I	CCTNAGG	1 cut(s)	79
BsuI	GTATCC	1 cut(s)	3577
BsuRI	GGCC	9 cut(s)	1562, 2783, 3263, 3805, 4009, 4312, 4406, 4431, 4649
BtgI	CCRYGG	7 cut(s)	12, 1786, 1959, 2570, 2727, 3668, 4385
BtsI	GCAGTG	3 cut(s)	2472, 3662, 4686
BtsIMutI	CAGTG	9 cut(s)	174, 1732, 2472, 3103, 3524, 3662, 3764, 4427, 4686
BveI	ACCTGC	2 cut(s)	622, 1198
CaiI	CAGNNNCTG	2 cut(s)	2025, 4319
CciI	TCATGA	1 cut(s)	2851
CfoI	GCGC	5 cut(s)	661, 1831, 3985, 4413, 4556
Cfr10I	RCCGGY	4 cut(s)	64, 943, 1013, 3256
Cfr13I	GGNCC	7 cut(s)	340, 1451, 1729, 1964, 2999, 3695, 4429
Cfr42I	CCGCGG	1 cut(s)	15
Cfr9I	CCCGGG	1 cut(s)	2690
CseI	GACGC	2 cut(s)	1100, 3428
CsiI	ACCWGGT	1 cut(s)	3903
DraI	TTTAAA	3 cut(s)	235, 2043, 2797
DraIII	CACNNNGTG	1 cut(s)	3115
DriI	GACNNNNNGTC	1 cut(s)	3867
EaeI	YGGCCR	5 cut(s)	3261, 3803, 4007, 4310, 4404
Eam1105I	GACNNNNNGTC	1 cut(s)	3867
EciI	GGCGGA	1 cut(s)	1005
Eco130I	CCWWGG	5 cut(s)	1786, 1959, 2727, 2784, 3668
Eco24I	GRGCYC	2 cut(s)	563, 4105
Eco31I	GGTCTC	2 cut(s)	29, 4566
Eco32I	GATATC	2 cut(s)	1255, 2557
Eco47I	GGWCC	6 cut(s)	340, 1451, 1729, 1964, 2999, 3695
Eco57I	CTGAAG	3 cut(s)	198, 3450, 3630
Eco81I	CCTNAGG	1 cut(s)	79
Eco88I	CYCGRG	3 cut(s)	1948, 2690, 3518
Eco91I	GGTNACC	3 cut(s)	402, 3899, 4193
EcoNI	CCTNNNNNAGG	1 cut(s)	78
EcoO109I	RGGNCCY	3 cut(s)	1964, 3695, 4429
EcoO65I	GGTNACC	3 cut(s)	402, 3899, 4193
EcoRI	GAATTC	3 cut(s)	1204, 2803, 4533
EcoRV	GATATC	2 cut(s)	1255, 2557
EcoT14I	CCWWGG	5 cut(s)	1786, 1959, 2727, 2784, 3668
EcoT22I	ATGCAT	1 cut(s)	4023
EcoT38I	GRGCYC	2 cut(s)	563, 4105
ErhI	CCWWGG	5 cut(s)	1786, 1959, 2727, 2784, 3668
FaqI	GGGAC	3 cut(s)	1977, 2856, 4674
FauI	CCCGC	2 cut(s)	204, 1986
FauNDI	CATATG	1 cut(s)	3971
FbaI	TGATCA	5 cut(s)	301, 640, 823, 2013, 4249
FblI	GTMKAC	2 cut(s)	2826, 3090
FriOI	GRGCYC	2 cut(s)	563, 4105
FspBI	CTAG	4 cut(s)	2193, 2607, 3306, 3389
FspI	TGCGCA	1 cut(s)	1830
GlaI	GCGC	5 cut(s)	660, 1830, 3984, 4412, 4555
GsaI	CCCAGC	3 cut(s)	412, 4151, 4489
GsuI	CTGGAG	6 cut(s)	1422, 2037, 2984, 3842, 3951, 4202
HaeII	RGCGCY	1 cut(s)	4414
HaeIII	GGCC	9 cut(s)	1562, 2783, 3263, 3805, 4009, 4312, 4406, 4431, 4649
HgaI	GACGC	2 cut(s)	1100, 3428
HhaI	GCGC	5 cut(s)	661, 1831, 3985, 4413, 4556
Hin6I	GCGC	5 cut(s)	659, 1829, 3983, 4411, 4554
HinP1I	GCGC	5 cut(s)	659, 1829, 3983, 4411, 4554
HincII	GTYRAC	1 cut(s)	4375
HindII	GTYRAC	1 cut(s)	4375
HindIII	AAGCTT	1 cut(s)	2677
HphI	GGTGA	6 cut(s)	821, 2096, 3506, 3919, 4205, 4360
HpyCH4IV	ACGT	1 cut(s)	3385
HpySE526I	ACGT	1 cut(s)	3385
HspAI	GCGC	5 cut(s)	659, 1829, 3983, 4411, 4554
KroI	GCCGGC	1 cut(s)	3256
KroNI	GCCGGC	1 cut(s)	3258
Ksp22I	TGATCA	5 cut(s)	301, 640, 823, 2013, 4249
KspI	CCGCGG	1 cut(s)	15
LguI	GCTCTTC	2 cut(s)	174, 3477
MabI	ACCWGGT	1 cut(s)	3903
MaeI	CTAG	4 cut(s)	2193, 2607, 3306, 3389
MaeII	ACGT	1 cut(s)	3385
MaeIII	GTNAC	9 cut(s)	402, 1372, 2170, 3115, 3187, 3522, 3899, 3951, 4193
MfeI	CAATTG	2 cut(s)	3471, 4134
MflI	RGATCY	3 cut(s)	2450, 4141, 4480
MhlI	GDGCHC	5 cut(s)	176, 563, 1847, 2513, 4105
MlsI	TGGCCA	1 cut(s)	4009
MluNI	TGGCCA	1 cut(s)	4009
MlyI	GAGTC	3 cut(s)	1310, 3530, 3868
MmeI	TCCRAC	4 cut(s)	405, 1314, 1837, 2250
Mox20I	TGGCCA	1 cut(s)	4009
Mph1103I	ATGCAT	1 cut(s)	4023
MroNI	GCCGGC	1 cut(s)	3256
MroXI	GAANNNNTTC	3 cut(s)	1341, 1614, 3728
MscI	TGGCCA	1 cut(s)	4009
MslI	CAYNNNNRTG	3 cut(s)	2856, 3667, 3970
Msp20I	TGGCCA	1 cut(s)	4009
MspA1I	CMGCKG	4 cut(s)	14, 23, 3980, 4151
MspCI	CTTAAG	1 cut(s)	3556
MunI	CAATTG	2 cut(s)	3471, 4134
Mva1269I	GAATGC	5 cut(s)	1482, 2702, 3776, 3965, 4556
MvnI	CGCG	2 cut(s)	14, 661
NaeI	GCCGGC	1 cut(s)	3258
NciI	CCSGG	4 cut(s)	2691, 2692, 3412, 4001
NcoI	CCATGG	4 cut(s)	1786, 1959, 2727, 3668
NdeI	CATATG	1 cut(s)	3971
NgoMIV	GCCGGC	1 cut(s)	3256
NheI	GCTAGC	1 cut(s)	2606
NmeAIII	GCCGAG	1 cut(s)	2863
NmuCI	GTSAC	5 cut(s)	3115, 3187, 3522, 3951, 4193
NsbI	TGCGCA	1 cut(s)	1830
NsiI	ATGCAT	1 cut(s)	4023
NspI	RCATGY	5 cut(s)	225, 442, 1288, 2274, 4025
NspV	TTCGAA	3 cut(s)	2063, 4084, 4259
PaeI	GCATGC	2 cut(s)	225, 1288
PagI	TCATGA	1 cut(s)	2851
PasI	CCCWGGG	1 cut(s)	1127
PciI	ACATGT	2 cut(s)	438, 2270
PciSI	GCTCTTC	2 cut(s)	174, 3477
PctI	GAATGC	5 cut(s)	1482, 2702, 3776, 3965, 4556
PdiI	GCCGGC	1 cut(s)	3258
PdmI	GAANNNNTTC	3 cut(s)	1341, 1614, 3728
PflMI	CCANNNNNTGG	2 cut(s)	4206, 4492
PfoI	TCCNGGA	3 cut(s)	1453, 3819, 3999
PleI	GAGTC	3 cut(s)	1310, 3529, 3868
PpsI	GAGTC	3 cut(s)	1310, 3529, 3868
PpuMI	RGGWCCY	2 cut(s)	1964, 3695
PscI	ACATGT	2 cut(s)	438, 2270
PshBI	ATTAAT	1 cut(s)	1155
Psp5II	RGGWCCY	2 cut(s)	1964, 3695
PspEI	GGTNACC	3 cut(s)	402, 3899, 4193
PspFI	CCCAGC	3 cut(s)	408, 4147, 4485
PspPI	GGNCC	7 cut(s)	340, 1451, 1729, 1964, 2999, 3695, 4429
PspPPI	RGGWCCY	2 cut(s)	1964, 3695
PstI	CTGCAG	2 cut(s)	781, 4096
PstNI	CAGNNNCTG	2 cut(s)	2025, 4319
PsuI	RGATCY	3 cut(s)	2450, 4141, 4480
PvuII	CAGCTG	2 cut(s)	3980, 4151
RseI	CAYNNNNRTG	3 cut(s)	2856, 3667, 3970
SacII	CCGCGG	1 cut(s)	15
SapI	GCTCTTC	2 cut(s)	174, 3477
Sau96I	GGNCC	7 cut(s)	340, 1451, 1729, 1964, 2999, 3695, 4429
ScaI	AGTACT	4 cut(s)	1085, 2036, 2843, 4090
SchI	GAGTC	3 cut(s)	1310, 3530, 3868
SduI	GDGCHC	5 cut(s)	176, 563, 1847, 2513, 4105
SexAI	ACCWGGT	1 cut(s)	3903
SfcI	CTRYAG	7 cut(s)	318, 625, 777, 2259, 3094, 4092, 4152
Sfr303I	CCGCGG	1 cut(s)	15
SfuI	TTCGAA	3 cut(s)	2063, 4084, 4259
SgrBI	CCGCGG	1 cut(s)	15
SinI	GGWCC	6 cut(s)	340, 1451, 1729, 1964, 2999, 3695
SmaI	CCCGGG	1 cut(s)	2692
SmiI	ATTTAAAT	1 cut(s)	2043
SmiMI	CAYNNNNRTG	3 cut(s)	2856, 3667, 3970
SmlI	CTYRAG	7 cut(s)	804, 1318, 1489, 3556, 3890, 4563, 4670
SmoI	CTYRAG	7 cut(s)	804, 1318, 1489, 3556, 3890, 4563, 4670
SphI	GCATGC	2 cut(s)	225, 1288
SspI	AATATT	1 cut(s)	2666
SspMI	CTAG	4 cut(s)	2193, 2607, 3306, 3389
StyI	CCWWGG	5 cut(s)	1786, 1959, 2727, 2784, 3668
SwaI	ATTTAAAT	1 cut(s)	2043
TaiI	ACGT	1 cut(s)	3388
TaqII	GACCGA	1 cut(s)	4548
TatI	WGTACW	6 cut(s)	1083, 1305, 2034, 2583, 2841, 4088
TauI	GCSGC	5 cut(s)	14, 26, 1419, 3263, 4312
TscAI	CASTG	9 cut(s)	181, 1739, 2479, 3103, 3531, 3669, 3764, 4427, 4686
TseFI	GTSAC	5 cut(s)	3115, 3187, 3522, 3951, 4193
Tsp45I	GTSAC	5 cut(s)	3115, 3187, 3522, 3951, 4193
TspGWI	ACGGA	4 cut(s)	2189, 3857, 3970, 4100
TspMI	CCCGGG	1 cut(s)	2690
TspRI	CASTG	9 cut(s)	181, 1739, 2479, 3103, 3531, 3669, 3764, 4427, 4686
Van91I	CCANNNNNTGG	2 cut(s)	4206, 4492
Vha464I	CTTAAG	1 cut(s)	3556
VneI	GTGCAC	1 cut(s)	2509
VpaK11BI	GGWCC	6 cut(s)	340, 1451, 1729, 1964, 2999, 3695
VspI	ATTAAT	1 cut(s)	1155
XagI	CCTNNNNNAGG	1 cut(s)	78
XapI	RAATTY	9 cut(s)	160, 761, 791, 1204, 1817, 2319, 2803, 2926, 4533
XceI	RCATGY	5 cut(s)	225, 442, 1288, 2274, 4025
XcmI	CCANNNNNNNNNTGG	5 cut(s)	1966, 2383, 3713, 3911, 4320
XmaI	CCCGGG	1 cut(s)	2690
XmiI	GTMKAC	2 cut(s)	2826, 3090
XmnI	GAANNNNTTC	3 cut(s)	1341, 1614, 3728
XspI	CTAG	4 cut(s)	2193, 2607, 3306, 3389
ZrmI	AGTACT	4 cut(s)	1085, 2036, 2843, 4090
Zsp2I	ATGCAT	1 cut(s)	4023

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.