AT4G16765

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0001740
Physical Location & Seq
Reverse (-)
9429629 .. 9432779
3151 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G16765.1

Sequence Viewer

Length: 744 bp
ATGGCTTTACTCCGCCGTGATTTGCTCGGTTATACTCCGTTGTATGCTGAGAAACTTGACCCGTCCTTGAGCTCCATAGGTGATTCCAAGGAAAGCTTCTATTTTGGCTCTTTGGAAGGTGTTCTAGCTCAACGTTACCCTAACCAATGGCCTTCTGAAGGTATCTTGCCATCATGGAGGCAAACCATGGAAACTTACTACAAGAATGTCCTGTCCGTGGGTAGAAAATTGCTTGGCTTGATTGCCTTAGCATTGGATTTAGATGAGGACTTCTTCGAAAAAGTTGGAGCCTTGAATGATCCTACAGCAGTTGTTCGCCTCTTACGATATCCAGGTGAAGTGATTTCGTCAGATGTTGAAACGTATGGTGCCTCAGCTCACTCAGATTATGGAATGGTCACTCTTCTTTTGACTGATGGAGTTCCAGGACTTCAGGTTTGTAGAGACAAATCGAAACAACCACACATTTGGGAAGATGTCCCTGGAATTAAAGGGGCGTTTATAGTCAACATTGGTGATATGATGGAGAGATGGACCAATGGATTGTTTCGGTCTACATTGCACAGAGTGATGCCGGTGGGAAAAGAACGTTACTCGGTGGTGTTCTTCTTAGATCCCAATCCAGATTGTAATGTGAAATGCTTGGAGAGTTGTTGCAGCGAAACTTGTCCCCCAAGATTCCCGCCTATTCTCGCCGGCGACTATATCAAGGAGCGTTTCAGGCTAACATACGCCACTTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

247

Amino Acids

27.75

Weight (kDa)

5.33

Isoelectric Point (pI)

38.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 109 - 206 8.5e-24 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 368
AccI GTMKAC 1 cut(s) 554
AciI CCGC 2 cut(s) 13, 683
AclI AACGTT 2 cut(s) 133, 589
AclWI GGATC 2 cut(s) 293, 608
AcuI CTGAAG 2 cut(s) 177, 416
AdeI CACNNNGTG 1 cut(s) 568
AfiI CCNNNNNNNGG 2 cut(s) 158, 217
AgsI TTSAA 2 cut(s) 295, 359
AjnI CCWGG 3 cut(s) 331, 424, 481
AjuI GAANNNNNNNTTGG 2 cut(s) 80, 112
AluBI AGCT 4 cut(s) 72, 96, 128, 377
AluI AGCT 4 cut(s) 72, 96, 128, 377
Alw21I GWGCWC 1 cut(s) 74
Alw26I GTCTC 1 cut(s) 438
AlwI GGATC 2 cut(s) 293, 608
AoxI GGCC 1 cut(s) 149
ApeKI GCWGC 1 cut(s) 657
ArsI GACNNNNNNTTYG 2 cut(s) 420, 452
Asp700I GAANNNNTTC 1 cut(s) 120
AspS9I GGNCC 1 cut(s) 534
AsuHPI GGTGA 3 cut(s) 92, 347, 527
AsuII TTCGAA 1 cut(s) 276
AvaII GGWCC 1 cut(s) 534
BanI GGYRCC 1 cut(s) 368
BanII GRGCYC 1 cut(s) 74
Bbv12I GWGCWC 1 cut(s) 74
BbvCI CCTCAGC 1 cut(s) 373
BbvI GCAGC 1 cut(s) 669
BccI CCATC 4 cut(s) 178, 410, 517, 525
BciT130I CCWGG 3 cut(s) 333, 426, 483
BcoDI GTCTC 1 cut(s) 438
BfaI CTAG 2 cut(s) 125, 742
BfmI CTRYAG 1 cut(s) 303
BisI GCNGC 1 cut(s) 658
BlsI GCNGC 1 cut(s) 659
Bme1390I CCNGG 3 cut(s) 333, 426, 483
Bme18I GGWCC 1 cut(s) 534
BmgT120I GGNCC 1 cut(s) 534
BmiI GGNNCC 2 cut(s) 289, 370
BmrFI CCNGG 3 cut(s) 333, 426, 483
BmsI GCATC 1 cut(s) 561
Bpu10I CCTNAGC 2 cut(s) 247, 373
Bpu14I TTCGAA 1 cut(s) 276
BpuEI CTTGAG 1 cut(s) 88
BsaBI GATNNNNATC 1 cut(s) 618
BsaJI CCNNGG 4 cut(s) 87, 186, 216, 481
Bsc4I CCNNNNNNNGG 2 cut(s) 158, 217
Bse118I RCCGGY 2 cut(s) 574, 695
Bse3DI GCAATG 1 cut(s) 557
Bse8I GATNNNNATC 1 cut(s) 618
BseBI CCWGG 3 cut(s) 333, 426, 483
BseDI CCNNGG 4 cut(s) 87, 186, 216, 481
BseJI GATNNNNATC 1 cut(s) 618
BseLI CCNNNNNNNGG 2 cut(s) 158, 217
BseMI GCAATG 1 cut(s) 557
BseMII CTCAG 3 cut(s) 39, 387, 396
BseXI GCAGC 1 cut(s) 669
BshFI GGCC 1 cut(s) 151
BshNI GGYRCC 1 cut(s) 368
BsiHKAI GWGCWC 1 cut(s) 74
BsiSI CCGG 2 cut(s) 575, 696
BslFI GGGAC 2 cut(s) 464, 654
BslI CCNNNNNNNGG 2 cut(s) 158, 217
BsmAI GTCTC 1 cut(s) 438
BsmFI GGGAC 2 cut(s) 464, 654
BsnI GGCC 1 cut(s) 151
Bsp119I TTCGAA 1 cut(s) 276
Bsp1286I GDGCHC 1 cut(s) 74
Bsp143I GATC 2 cut(s) 298, 613
Bsp19I CCATGG 1 cut(s) 186
BspACI CCGC 2 cut(s) 13, 683
BspANI GGCC 1 cut(s) 151
BspCNI CTCAG 3 cut(s) 40, 386, 395
BspLI GGNNCC 2 cut(s) 289, 370
BspPI GGATC 2 cut(s) 293, 608
BspT104I TTCGAA 1 cut(s) 276
BspT107I GGYRCC 1 cut(s) 368
BsrDI GCAATG 1 cut(s) 557
BsrFI RCCGGY 2 cut(s) 574, 695
BssAI RCCGGY 2 cut(s) 574, 695
BssECI CCNNGG 4 cut(s) 87, 186, 216, 481
BssMI GATC 2 cut(s) 298, 613
BssT1I CCWWGG 2 cut(s) 87, 186
Bst2UI CCWGG 3 cut(s) 333, 426, 483
Bst6I CTCTTC 1 cut(s) 408
BstBI TTCGAA 1 cut(s) 276
BstC8I GCNNGC 1 cut(s) 697
BstDEI CTNAG 5 cut(s) 48, 247, 373, 382, 610
BstDSI CCRYGG 2 cut(s) 186, 216
BstENI CCTNNNNNAGG 1 cut(s) 156
BstKTI GATC 2 cut(s) 301, 616
BstMAI GTCTC 1 cut(s) 438
BstMBI GATC 2 cut(s) 298, 613
BstMWI GCNNNNNNNGC 1 cut(s) 721
BstNI CCWGG 3 cut(s) 333, 426, 483
BstSCI CCNGG 3 cut(s) 331, 424, 481
BstSFI CTRYAG 1 cut(s) 303
BstV1I GCAGC 1 cut(s) 669
BstX2I RGATCY 1 cut(s) 613
BstXI CCANNNNNNTGG 1 cut(s) 468
BstYI RGATCY 1 cut(s) 613
BsuRI GGCC 1 cut(s) 151
BtgI CCRYGG 2 cut(s) 186, 216
Cac8I GCNNGC 1 cut(s) 697
Cfr10I RCCGGY 2 cut(s) 574, 695
Cfr13I GGNCC 1 cut(s) 534
CviAII CATG 2 cut(s) 174, 187
DdeI CTNAG 5 cut(s) 48, 247, 373, 382, 610
DpnI GATC 2 cut(s) 300, 615
DpnII GATC 2 cut(s) 298, 613
DraIII CACNNNGTG 1 cut(s) 568
Eam1104I CTCTTC 1 cut(s) 408
EarI CTCTTC 1 cut(s) 408
Ecl136II GAGCTC 1 cut(s) 72
Eco130I CCWWGG 2 cut(s) 87, 186
Eco24I GRGCYC 1 cut(s) 74
Eco32I GATATC 1 cut(s) 329
Eco47I GGWCC 1 cut(s) 534
Eco53kI GAGCTC 1 cut(s) 72
Eco57I CTGAAG 2 cut(s) 177, 416
EcoICRI GAGCTC 1 cut(s) 72
EcoNI CCTNNNNNAGG 1 cut(s) 156
EcoRII CCWGG 3 cut(s) 331, 424, 481
EcoRV GATATC 1 cut(s) 329
EcoT14I CCWWGG 2 cut(s) 87, 186
EcoT38I GRGCYC 1 cut(s) 74
ErhI CCWWGG 2 cut(s) 87, 186
FaeI CATG 2 cut(s) 177, 190
FalI AAGNNNNNCTT 2 cut(s) 80, 112
FaqI GGGAC 2 cut(s) 464, 654
FatI CATG 2 cut(s) 173, 186
FauI CCCGC 1 cut(s) 690
FblI GTMKAC 1 cut(s) 554
Fnu4HI GCNGC 1 cut(s) 658
FriOI GRGCYC 1 cut(s) 74
Fsp4HI GCNGC 1 cut(s) 658
FspBI CTAG 2 cut(s) 125, 742
GluI GCNGC 1 cut(s) 658
HaeIII GGCC 1 cut(s) 151
HapII CCGG 2 cut(s) 575, 696
Hin1II CATG 2 cut(s) 177, 190
HincII GTYRAC 1 cut(s) 508
HindII GTYRAC 1 cut(s) 508
HindIII AAGCTT 1 cut(s) 94
HinfI GANTC 2 cut(s) 83, 678
HpaII CCGG 2 cut(s) 575, 696
HphI GGTGA 3 cut(s) 92, 347, 527
Hpy166II GTNNAC 2 cut(s) 508, 555
Hpy188I TCNGA 3 cut(s) 157, 352, 385
Hpy188III TCNNGA 1 cut(s) 623
Hpy8I GTNNAC 2 cut(s) 508, 555
HpyAV CCTTC 3 cut(s) 110, 152, 162
HpyCH4IV ACGT 3 cut(s) 133, 362, 589
HpyCH4V TGCA 2 cut(s) 562, 657
HpyF10VI GCNNNNNNNGC 1 cut(s) 721
HpyF3I CTNAG 5 cut(s) 48, 247, 373, 382, 610
HpySE526I ACGT 3 cut(s) 133, 362, 589
Hsp92II CATG 2 cut(s) 177, 190
KroI GCCGGC 1 cut(s) 695
KroNI GCCGGC 1 cut(s) 697
Kzo9I GATC 2 cut(s) 298, 613
LmnI GCTCC 3 cut(s) 77, 287, 712
Lsp1109I GCAGC 1 cut(s) 669
LweI GCATC 1 cut(s) 561
MaeI CTAG 2 cut(s) 125, 742
MaeII ACGT 3 cut(s) 133, 362, 589
MaeIII GTNAC 3 cut(s) 134, 397, 590
MalI GATC 2 cut(s) 300, 615
MboI GATC 2 cut(s) 298, 613
MboII GAAGA 4 cut(s) 265, 395, 485, 598
MflI RGATCY 1 cut(s) 613
MhlI GDGCHC 1 cut(s) 74
MluCI AATT 2 cut(s) 227, 486
MmeI TCCRAC 1 cut(s) 265
MnlI CCTC 4 cut(s) 171, 259, 329, 382
MreI CGCCGGCG 1 cut(s) 695
MroNI GCCGGC 1 cut(s) 695
MroXI GAANNNNTTC 1 cut(s) 120
MseI TTAA 1 cut(s) 489
MspI CCGG 2 cut(s) 575, 696
MspR9I CCNGG 3 cut(s) 333, 426, 483
MvaI CCWGG 3 cut(s) 333, 426, 483
MwoI GCNNNNNNNGC 1 cut(s) 721
NaeI GCCGGC 1 cut(s) 697
NcoI CCATGG 1 cut(s) 186
NdeII GATC 2 cut(s) 298, 613
NgoMIV GCCGGC 1 cut(s) 695
NlaIII CATG 2 cut(s) 177, 190
NlaIV GGNNCC 2 cut(s) 289, 370
NmuCI GTSAC 1 cut(s) 397
NspV TTCGAA 1 cut(s) 276
PcsI WCGNNNNNNNCGW 1 cut(s) 322
PdiI GCCGGC 1 cut(s) 697
PdmI GAANNNNTTC 1 cut(s) 120
PfeI GAWTC 2 cut(s) 83, 678
PfoI TCCNGGA 1 cut(s) 424
PkrI GCNGC 1 cut(s) 659
Psp124BI GAGCTC 1 cut(s) 74
Psp1406I AACGTT 2 cut(s) 133, 589
Psp6I CCWGG 3 cut(s) 331, 424, 481
PspGI CCWGG 3 cut(s) 331, 424, 481
PspN4I GGNNCC 2 cut(s) 289, 370
PspPI GGNCC 1 cut(s) 534
PsuI RGATCY 1 cut(s) 613
SacI GAGCTC 1 cut(s) 74
SaqAI TTAA 1 cut(s) 489
SatI GCNGC 1 cut(s) 658
Sau3AI GATC 2 cut(s) 298, 613
Sau96I GGNCC 1 cut(s) 534
ScrFI CCNGG 3 cut(s) 333, 426, 483
SduI GDGCHC 1 cut(s) 74
SfaNI GCATC 1 cut(s) 561
SfcI CTRYAG 1 cut(s) 303
SfuI TTCGAA 1 cut(s) 276
SgrAI CRCCGGYG 1 cut(s) 695
SinI GGWCC 1 cut(s) 534
SmlI CTYRAG 1 cut(s) 67
SmoI CTYRAG 1 cut(s) 67
Sse9I AATT 2 cut(s) 227, 486
SsiI CCGC 2 cut(s) 13, 683
SspMI CTAG 2 cut(s) 125, 742
SstI GAGCTC 1 cut(s) 74
StyD4I CCNGG 3 cut(s) 331, 424, 481
StyI CCWWGG 2 cut(s) 87, 186
TaiI ACGT 3 cut(s) 136, 365, 592
TaqI TCGA 2 cut(s) 276, 452
TaqII GACCGA 1 cut(s) 540
TasI AATT 2 cut(s) 227, 486
TfiI GAWTC 2 cut(s) 83, 678
Tru1I TTAA 1 cut(s) 489
Tru9I TTAA 1 cut(s) 489
TseFI GTSAC 1 cut(s) 397
TseI GCWGC 1 cut(s) 657
Tsp45I GTSAC 1 cut(s) 397
TspGWI ACGGA 2 cut(s) 27, 205
VpaK11BI GGWCC 1 cut(s) 534
XagI CCTNNNNNAGG 1 cut(s) 156
XmiI GTMKAC 1 cut(s) 554
XmnI GAANNNNTTC 1 cut(s) 120
XspI CTAG 2 cut(s) 125, 742
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.