Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0001740

Physical Location & Seq

Genomic Coordinates Reverse (-)

7995325 .. 7998512

Gene Structure 3188 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1060700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 948 bp

ATGACGACGGAGGCGGTGAACCTGCCGATCATTGACTTGTCCTCATCAGATTCCATCTCCTCTGCCAATTCAATCCGTCAGGCATGCATAGAATATGGTTTCTTCTACCTGGTGAATCATGGCGTCGAGGAAGAGCTGTTTGCAGAGGTGTTCGATCGGAGCCGCAACTTCTTTTCGCTTCCTCTGGAAGATAAGTTGAAATTGACTCGCAAGCACCACAGAGGCTACACCCCACTATTTATGGAGAATCTCGACTTCGCTTCCAGCTCCAAAGGTGACTCGAAAGAGAGTTTTTATGTTGGCTCTTTAGATGACCCAACTCTGATTAATTTGAACCAATGGCCTCCAGAAGATATCCTGCCATCTTGGAGGCCTGCAATGGAATTGTACTATAGAAAAGTCCTGTCTGCTGGAAAAAGATTAATCTCTCTGATTGCCCTGGCTTTAAACCTGGATGAGGACTTTTTCGAGAAAGTGGGGGCACTGAATAAACCAAGGGGTCTTCTTCGACTTTTGCACTATCCAGGTGAGCTGGGATCAACTGACGAAGAAATATATGGTGCTTCTGCTCATTCAGATTATGGCATGGTCACTCTCCTTGCATCCAATGGAGTTCCTGGACTTCAGGCATGCTTTTTGTTTGTTTGTAGGGACAAATCTAAGCAACCTCGAATCTGGGAAGATGTTCTTCACATAGATGGGGCATTCATTGTCAACATTGGGGACATGATGGAGAGGTGGACTAATTGTTTGTTCAGGTCAACACTGCATAGAGTGATGCCAGCAGGACAAGAACGCTACTCCGTGGCTTTCTTCATGGATCCCAATGAAGATTGTGTGGTGGAATGTCTGAAAAGTTGTTGCAGTGAAGAATCACCGGCAAGATTTCCTCCCGTCCGCAGCGGGGACTACCTGGAAGAGCGTTTTCGGCTTACGTATGCAACTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

2OG-FeII_Oxy DIOX_N

Protein Analysis

316

Amino Acids

35.79

Weight (kDa)

5.06

Isoelectric Point (pI)

54.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DIOX_N	PF14226	8 - 117	7.3e-30	non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy	PF03171	168 - 275	8.9e-19	2OG-Fe(II) oxygenase superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0001740)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G16765 AT4G16765 AT4G16765 AT4G16765 AT4G16765 AT4G16765 AT4G16770
fragaria_vesca	FvH4_5g19520 FvH4_5g19520 FvH4_5g19520 FvH4_5g19520 FvH4_5g19520 FvH4_5g19520 FvH4_5g19520 FvH4_5g19520 FvH4_5g19520 FvH4_5g19520 FvH4_5g19520 FvH4_5g19520 FvH4_5g19520
malus_domestica	MD00G1169400.v1.1 MD00G1169500.v1.1 MD04G1060700.v1.1 MD04G1060800.v1.1 MD11G1277300.v1.1
prunus_persica	Prupe.5G064700_v2.0.a1
pyrus_communis	pycom04g05460 pycom04g05470
rosa_chinensis	RchiOBHm_Chr7g0204121 RchiOBHm_Chr7g0204131
rosa_laevigata	RLG00000003486 RLG00000003488
rosa_multiflora	Rmu_sc0001311.1_g000016 Rmu_sc0001311.1_g000018
rosa_roxburghii	Rroxscaffold_3G00253320 Rroxscaffold_3G00253340
rosa_rugosa	Rorug07G0080800 Rorug07G0080900 Rorug07G0081000
rosa_samantha	Rh7AG210800 Rh7BG209300 Rh7BG209500 Rh7CG223200 Rh7CG223400 Rh7DG217500 Rh7DG217700
rosa_wichuraiana	Rw7G018340 Rw7G018350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	30
AciI	CCGC	4 cut(s)	14, 163, 898, 903
AclWI	GGATC	3 cut(s)	544, 815, 828
AcuI	CTGAAG	1 cut(s)	608
AcyI	GRCGYC	1 cut(s)	123
AfaI	GTAC	1 cut(s)	389
AfiI	CCNNNNNNNGG	2 cut(s)	457, 904
AgsI	TTSAA	3 cut(s)	72, 199, 334
AjnI	CCWGG	6 cut(s)	108, 438, 450, 523, 616, 912
AluBI	AGCT	3 cut(s)	136, 267, 532
AluI	AGCT	3 cut(s)	136, 267, 532
AlwI	GGATC	3 cut(s)	544, 815, 828
AoxI	GGCC	2 cut(s)	341, 371
ApeKI	GCWGC	1 cut(s)	900
ArsI	GACNNNNNNTTYG	2 cut(s)	733, 765
AseI	ATTAAT	2 cut(s)	327, 422
Asp700I	GAANNNNTTC	1 cut(s)	684
AsuHPI	GGTGA	5 cut(s)	28, 124, 287, 539, 867
BaeGI	GKGCMC	1 cut(s)	484
BamHI	GGATCC	1 cut(s)	820
BbsI	GAAGAC	1 cut(s)	494
BbvI	GCAGC	1 cut(s)	912
BccI	CCATC	4 cut(s)	62, 370, 692, 724
BciT130I	CCWGG	6 cut(s)	110, 440, 452, 525, 618, 914
BfmI	CTRYAG	1 cut(s)	391
BfuAI	ACCTGC	1 cut(s)	30
BisI	GCNGC	2 cut(s)	163, 901
BlsI	GCNGC	2 cut(s)	164, 902
Bme1390I	CCNGG	6 cut(s)	110, 440, 452, 525, 618, 914
BmiI	GGNNCC	2 cut(s)	161, 822
BmrFI	CCNGG	6 cut(s)	110, 440, 452, 525, 618, 914
BmsI	GCATC	2 cut(s)	611, 768
BpiI	GAAGAC	1 cut(s)	494
BpmI	CTGGAG	1 cut(s)	330
BsaAI	YACGTR	1 cut(s)	936
BsaHI	GRCGYC	1 cut(s)	123
BsaJI	CCNNGG	3 cut(s)	438, 494, 804
Bsc4I	CCNNNNNNNGG	2 cut(s)	457, 904
Bse118I	RCCGGY	1 cut(s)	877
Bse3DI	GCAATG	1 cut(s)	384
BseBI	CCWGG	6 cut(s)	110, 440, 452, 525, 618, 914
BseDI	CCNNGG	3 cut(s)	438, 494, 804
BseGI	GGATG	2 cut(s)	460, 602
BseLI	CCNNNNNNNGG	2 cut(s)	457, 904
BseMI	GCAATG	1 cut(s)	384
BseRI	GAGGAG	1 cut(s)	49
BseSI	GKGCMC	1 cut(s)	484
BseXI	GCAGC	1 cut(s)	912
BseYI	CCCAGC	1 cut(s)	532
Bsh1285I	CGRYCG	1 cut(s)	157
BshFI	GGCC	2 cut(s)	343, 373
BsiEI	CGRYCG	1 cut(s)	157
BsiSI	CCGG	1 cut(s)	878
BslFI	GGGAC	3 cut(s)	665, 737, 920
BslI	CCNNNNNNNGG	2 cut(s)	457, 904
BsmFI	GGGAC	3 cut(s)	665, 737, 920
BsmI	GAATGC	1 cut(s)	704
BsnI	GGCC	2 cut(s)	343, 373
Bsp1286I	GDGCHC	1 cut(s)	484
Bsp143I	GATC	4 cut(s)	27, 154, 536, 820
BspACI	CCGC	4 cut(s)	14, 163, 898, 903
BspANI	GGCC	2 cut(s)	343, 373
BspLI	GGNNCC	2 cut(s)	161, 822
BspMI	ACCTGC	1 cut(s)	30
BspPI	GGATC	3 cut(s)	544, 815, 828
BspQI	GCTCTTC	2 cut(s)	126, 912
BsrDI	GCAATG	1 cut(s)	384
BsrFI	RCCGGY	1 cut(s)	877
BssAI	RCCGGY	1 cut(s)	877
BssECI	CCNNGG	3 cut(s)	438, 494, 804
BssMI	GATC	4 cut(s)	27, 154, 536, 820
BssNI	GRCGYC	1 cut(s)	123
BssT1I	CCWWGG	1 cut(s)	494
Bst2UI	CCWGG	6 cut(s)	110, 440, 452, 525, 618, 914
Bst6I	CTCTTC	2 cut(s)	126, 912
BstACI	GRCGYC	1 cut(s)	123
BstBAI	YACGTR	1 cut(s)	936
BstC8I	GCNNGC	5 cut(s)	85, 212, 375, 631, 783
BstDEI	CTNAG	1 cut(s)	660
BstDSI	CCRYGG	1 cut(s)	804
BstENI	CCTNNNNNAGG	1 cut(s)	455
BstF5I	GGATG	2 cut(s)	460, 602
BstKTI	GATC	4 cut(s)	30, 157, 539, 823
BstMBI	GATC	4 cut(s)	27, 154, 536, 820
BstMCI	CGRYCG	1 cut(s)	157
BstMWI	GCNNNNNNNGC	1 cut(s)	928
BstNI	CCWGG	6 cut(s)	110, 440, 452, 525, 618, 914
BstNSI	RCATGY	2 cut(s)	87, 633
BstSCI	CCNGG	6 cut(s)	108, 438, 450, 523, 616, 912
BstSFI	CTRYAG	1 cut(s)	391
BstSLI	GKGCMC	1 cut(s)	484
BstSNI	TACGTA	1 cut(s)	936
BstV1I	GCAGC	1 cut(s)	912
BstV2I	GAAGAC	1 cut(s)	494
BstX2I	RGATCY	1 cut(s)	820
BstYI	RGATCY	1 cut(s)	820
BsuRI	GGCC	2 cut(s)	343, 373
BtgI	CCRYGG	1 cut(s)	804
BtsCI	GGATG	2 cut(s)	460, 602
BtsI	GCAGTG	2 cut(s)	764, 871
BtsIMutI	CAGTG	3 cut(s)	482, 764, 871
BveI	ACCTGC	1 cut(s)	30
Cac8I	GCNNGC	5 cut(s)	85, 212, 375, 631, 783
Cfr10I	RCCGGY	1 cut(s)	877
CseI	GACGC	1 cut(s)	112
CsiI	ACCWGGT	1 cut(s)	108
Csp6I	GTAC	1 cut(s)	388
CviAII	CATG	6 cut(s)	84, 119, 586, 630, 727, 817
CviQI	GTAC	1 cut(s)	388
DdeI	CTNAG	1 cut(s)	660
DpnI	GATC	4 cut(s)	29, 156, 538, 822
DpnII	GATC	4 cut(s)	27, 154, 536, 820
DraI	TTTAAA	1 cut(s)	447
Eam1104I	CTCTTC	2 cut(s)	126, 912
EarI	CTCTTC	2 cut(s)	126, 912
Eco105I	TACGTA	1 cut(s)	936
Eco130I	CCWWGG	1 cut(s)	494
Eco147I	AGGCCT	1 cut(s)	373
Eco32I	GATATC	1 cut(s)	355
Eco57I	CTGAAG	1 cut(s)	608
EcoNI	CCTNNNNNAGG	1 cut(s)	455
EcoRII	CCWGG	6 cut(s)	108, 438, 450, 523, 616, 912
EcoRV	GATATC	1 cut(s)	355
EcoT14I	CCWWGG	1 cut(s)	494
EcoT22I	ATGCAT	1 cut(s)	89
ErhI	CCWWGG	1 cut(s)	494
FaeI	CATG	6 cut(s)	87, 122, 589, 633, 730, 820
FalI	AAGNNNNNCTT	2 cut(s)	672, 704
FaqI	GGGAC	3 cut(s)	665, 737, 920
FatI	CATG	6 cut(s)	83, 118, 585, 629, 726, 816
FauI	CCCGC	1 cut(s)	896
Fnu4HI	GCNGC	2 cut(s)	163, 901
FokI	GGATG	2 cut(s)	467, 589
Fsp4HI	GCNGC	2 cut(s)	163, 901
GluI	GCNGC	2 cut(s)	163, 901
GsaI	CCCAGC	1 cut(s)	536
GsuI	CTGGAG	1 cut(s)	330
HaeIII	GGCC	2 cut(s)	343, 373
HapII	CCGG	1 cut(s)	878
HgaI	GACGC	1 cut(s)	112
Hin1I	GRCGYC	1 cut(s)	123
Hin1II	CATG	6 cut(s)	87, 122, 589, 633, 730, 820
HincII	GTYRAC	2 cut(s)	715, 762
HindII	GTYRAC	2 cut(s)	715, 762
HinfI	GANTC	7 cut(s)	50, 115, 205, 247, 278, 672, 872
HpaII	CCGG	1 cut(s)	878
HphI	GGTGA	5 cut(s)	28, 124, 287, 539, 867
Hpy166II	GTNNAC	4 cut(s)	19, 715, 741, 762
Hpy188I	TCNGA	6 cut(s)	49, 159, 324, 432, 577, 852
Hpy188III	TCNNGA	4 cut(s)	185, 251, 347, 469
Hpy8I	GTNNAC	4 cut(s)	19, 715, 741, 762
Hpy99I	CGWCG	2 cut(s)	10, 128
HpyCH4IV	ACGT	1 cut(s)	935
HpyCH4V	TGCA	8 cut(s)	87, 143, 377, 517, 602, 769, 864, 941
HpyF10VI	GCNNNNNNNGC	1 cut(s)	928
HpyF3I	CTNAG	1 cut(s)	660
HpySE526I	ACGT	1 cut(s)	935
Hsp92I	GRCGYC	1 cut(s)	123
Hsp92II	CATG	6 cut(s)	87, 122, 589, 633, 730, 820
Kzo9I	GATC	4 cut(s)	27, 154, 536, 820
LguI	GCTCTTC	2 cut(s)	126, 912
LmnI	GCTCC	2 cut(s)	159, 272
Lsp1109I	GCAGC	1 cut(s)	912
LweI	GCATC	2 cut(s)	611, 768
MabI	ACCWGGT	1 cut(s)	108
MaeII	ACGT	1 cut(s)	935
MaeIII	GTNAC	2 cut(s)	275, 589
MalI	GATC	4 cut(s)	29, 156, 538, 822
MboI	GATC	4 cut(s)	27, 154, 536, 820
MflI	RGATCY	1 cut(s)	820
MhlI	GDGCHC	1 cut(s)	484
MluCI	AATT	5 cut(s)	67, 200, 328, 383, 745
MlyI	GAGTC	2 cut(s)	199, 272
Mph1103I	ATGCAT	1 cut(s)	89
MroXI	GAANNNNTTC	1 cut(s)	684
MseI	TTAA	4 cut(s)	327, 422, 446, 946
MslI	CAYNNNNRTG	1 cut(s)	696
MspA1I	CMGCKG	1 cut(s)	903
MspI	CCGG	1 cut(s)	878
MspR9I	CCNGG	6 cut(s)	110, 440, 452, 525, 618, 914
Mva1269I	GAATGC	1 cut(s)	704
MvaI	CCWGG	6 cut(s)	110, 440, 452, 525, 618, 914
MwoI	GCNNNNNNNGC	1 cut(s)	928
NdeII	GATC	4 cut(s)	27, 154, 536, 820
NlaIII	CATG	6 cut(s)	87, 122, 589, 633, 730, 820
NlaIV	GGNNCC	2 cut(s)	161, 822
NmuCI	GTSAC	2 cut(s)	275, 589
NsiI	ATGCAT	1 cut(s)	89
NspI	RCATGY	2 cut(s)	87, 633
PaeI	GCATGC	2 cut(s)	87, 633
PceI	AGGCCT	1 cut(s)	373
PciSI	GCTCTTC	2 cut(s)	126, 912
PctI	GAATGC	1 cut(s)	704
PdmI	GAANNNNTTC	1 cut(s)	684
PfeI	GAWTC	5 cut(s)	50, 115, 247, 672, 872
PfoI	TCCNGGA	1 cut(s)	616
PkrI	GCNGC	2 cut(s)	164, 902
Ple19I	CGATCG	1 cut(s)	157
PleI	GAGTC	2 cut(s)	199, 272
PpsI	GAGTC	2 cut(s)	199, 272
Ppu21I	YACGTR	1 cut(s)	936
PshBI	ATTAAT	2 cut(s)	327, 422
Psp6I	CCWGG	6 cut(s)	108, 438, 450, 523, 616, 912
PspFI	CCCAGC	1 cut(s)	532
PspGI	CCWGG	6 cut(s)	108, 438, 450, 523, 616, 912
PspN4I	GGNNCC	2 cut(s)	161, 822
PsuI	RGATCY	1 cut(s)	820
PvuI	CGATCG	1 cut(s)	157
RsaI	GTAC	1 cut(s)	389
RsaNI	GTAC	1 cut(s)	388
RseI	CAYNNNNRTG	1 cut(s)	696
SapI	GCTCTTC	2 cut(s)	126, 912
SaqAI	TTAA	4 cut(s)	327, 422, 446, 946
SatI	GCNGC	2 cut(s)	163, 901
Sau3AI	GATC	4 cut(s)	27, 154, 536, 820
SchI	GAGTC	2 cut(s)	199, 272
ScrFI	CCNGG	6 cut(s)	110, 440, 452, 525, 618, 914
SduI	GDGCHC	1 cut(s)	484
SexAI	ACCWGGT	1 cut(s)	108
SfaNI	GCATC	2 cut(s)	611, 768
SfcI	CTRYAG	1 cut(s)	391
SmiMI	CAYNNNNRTG	1 cut(s)	696
SnaBI	TACGTA	1 cut(s)	936
SphI	GCATGC	2 cut(s)	87, 633
Sse9I	AATT	5 cut(s)	67, 200, 328, 383, 745
SseBI	AGGCCT	1 cut(s)	373
SsiI	CCGC	4 cut(s)	14, 163, 898, 903
StuI	AGGCCT	1 cut(s)	373
StyD4I	CCNGG	6 cut(s)	108, 438, 450, 523, 616, 912
StyI	CCWWGG	1 cut(s)	494
TaiI	ACGT	1 cut(s)	938
TaqI	TCGA	7 cut(s)	126, 153, 252, 281, 468, 508, 670
TasI	AATT	5 cut(s)	67, 200, 328, 383, 745
TatI	WGTACW	1 cut(s)	387
TauI	GCSGC	1 cut(s)	165
TfiI	GAWTC	5 cut(s)	50, 115, 247, 672, 872
Tru1I	TTAA	4 cut(s)	327, 422, 446, 946
Tru9I	TTAA	4 cut(s)	327, 422, 446, 946
TscAI	CASTG	3 cut(s)	489, 771, 871
TseFI	GTSAC	2 cut(s)	275, 589
TseI	GCWGC	1 cut(s)	900
Tsp45I	GTSAC	2 cut(s)	275, 589
TspDTI	ATGAA	3 cut(s)	697, 805, 843
TspGWI	ACGGA	3 cut(s)	23, 65, 793
TspRI	CASTG	3 cut(s)	489, 771, 871
VspI	ATTAAT	2 cut(s)	327, 422
XagI	CCTNNNNNAGG	1 cut(s)	455
XceI	RCATGY	2 cut(s)	87, 633
XmnI	GAANNNNTTC	1 cut(s)	684
Zsp2I	ATGCAT	1 cut(s)	89

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.