MD04G1060800.v1.1

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0001740
Physical Location & Seq
Reverse (-)
8013339 .. 8017031
3693 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1060800.v1.1.491

Sequence Viewer

Length: 714 bp
ATGGGCTTCAGAAGGAAATATACAGATATCCTTCCATCTTGGAGGCCTGCAGTGGAATCGTACTATAGAAAAGTCTGCTGGAAAAAGATTAATCTCTCCGATTGCGCCCTGGCTTTAAACCTGGATGAGGACTTTGTCGAGAAAGTGGGGGCAATGAATAAACCAAGGGTTTTTTTCTTCTACTTTTGTGATCTGGGATCAACGGATGAAGAAATTTATGGTGCTTCTGCTCATTCAGATTATGGCCTGGTCACACTCCTTGCATCCAATGGAGTTCCTGGACTTCAGGCATGCTTTCTGTTTGTTGGATTTTCTCAGCATCGAACACCTAGTGGTCCCCTTTCAAGCTCAATTATGATATTCTTTTCTGTGACTTCTTGTGAACACTTGGCAGGTTTGGCATTCATTGTTAACATTGGGGACATGATGGAGAGGTGGACTAATTATTTGGTCAGGTCAACACTGCATAGAGTGATGCCAGCAGGACAAGAACGCTACTCCGTGGCTCTCTTCATGAGTCCCAGTGAAGATTGTGTGGTGGAATGTCTGAAAAGTTGTTGCAGTGAAGAATCACCGCCAAGATTTCCTCCCGTCCGCAGCGGGGACTACCTGGAAGAGCGTTTTCGGCTTACACATGCAACTCAAAAGGTCTGTCTTTTGCAGTTGCAGTACATGGTTATGATTTTATTGTTTGGTGATAAACGGCTTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

238

Amino Acids

26.94

Weight (kDa)

6.52

Isoelectric Point (pI)

60.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 67 - 174 2e-11 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 383
AciI CCGC 3 cut(s) 575, 595, 600
AclWI GGATC 1 cut(s) 205
AcsI RAATTY 1 cut(s) 213
AcuI CTGAAG 1 cut(s) 269
AdeI CACNNNGTG 1 cut(s) 332
AfaI GTAC 2 cut(s) 62, 671
AfiI CCNNNNNNNGG 2 cut(s) 127, 601
AgsI TTSAA 1 cut(s) 345
AjnI CCWGG 5 cut(s) 108, 120, 246, 277, 609
AluBI AGCT 1 cut(s) 348
AluI AGCT 1 cut(s) 348
AlwI GGATC 1 cut(s) 205
AoxI GGCC 2 cut(s) 44, 244
ApeKI GCWGC 1 cut(s) 597
ApoI RAATTY 1 cut(s) 213
ArsI GACNNNNNNTTYG 4 cut(s) 430, 462, 637, 669
AseI ATTAAT 1 cut(s) 90
AspLEI GCGC 1 cut(s) 107
AspS9I GGNCC 1 cut(s) 335
AsuHPI GGTGA 2 cut(s) 564, 707
AvaII GGWCC 1 cut(s) 335
BarI GAAGNNNNNNTAC 1 cut(s) 36
BbvI GCAGC 1 cut(s) 609
BccI CCATC 2 cut(s) 43, 421
BcgI CGANNNNNNTGC 2 cut(s) 39, 73
BciT130I CCWGG 5 cut(s) 110, 122, 248, 279, 611
BfaI CTAG 1 cut(s) 330
BfmI CTRYAG 2 cut(s) 48, 64
BfuAI ACCTGC 1 cut(s) 383
BisI GCNGC 1 cut(s) 598
BlsI GCNGC 1 cut(s) 599
Bme1390I CCNGG 5 cut(s) 110, 122, 248, 279, 611
Bme18I GGWCC 1 cut(s) 335
BmgT120I GGNCC 1 cut(s) 335
BmiI GGNNCC 1 cut(s) 337
BmrFI CCNGG 5 cut(s) 110, 122, 248, 279, 611
BmrI ACTGGG 1 cut(s) 516
BmsI GCATC 3 cut(s) 272, 328, 465
BmuI ACTGGG 1 cut(s) 516
BsaJI CCNNGG 3 cut(s) 108, 164, 501
Bsc4I CCNNNNNNNGG 2 cut(s) 127, 601
Bse1I ACTGG 1 cut(s) 522
Bse3DI GCAATG 1 cut(s) 159
BseBI CCWGG 5 cut(s) 110, 122, 248, 279, 611
BseDI CCNNGG 3 cut(s) 108, 164, 501
BseGI GGATG 3 cut(s) 130, 211, 263
BseLI CCNNNNNNNGG 2 cut(s) 127, 601
BseMI GCAATG 1 cut(s) 159
BseMII CTCAG 1 cut(s) 329
BseNI ACTGG 1 cut(s) 522
BseXI GCAGC 1 cut(s) 609
BshFI GGCC 2 cut(s) 46, 246
BslFI GGGAC 4 cut(s) 321, 434, 504, 617
BslI CCNNNNNNNGG 2 cut(s) 127, 601
BsmFI GGGAC 4 cut(s) 321, 434, 504, 617
BsmI GAATGC 1 cut(s) 401
BsnI GGCC 2 cut(s) 46, 246
Bsp143I GATC 2 cut(s) 190, 197
BspACI CCGC 3 cut(s) 575, 595, 600
BspANI GGCC 2 cut(s) 46, 246
BspCNI CTCAG 1 cut(s) 328
BspHI TCATGA 1 cut(s) 513
BspLI GGNNCC 1 cut(s) 337
BspMAI CTGCAG 1 cut(s) 52
BspMI ACCTGC 1 cut(s) 383
BspPI GGATC 1 cut(s) 205
BspQI GCTCTTC 1 cut(s) 609
BsrDI GCAATG 1 cut(s) 159
BsrI ACTGG 1 cut(s) 522
BssECI CCNNGG 3 cut(s) 108, 164, 501
BssMI GATC 2 cut(s) 190, 197
BssT1I CCWWGG 1 cut(s) 164
Bst2UI CCWGG 5 cut(s) 110, 122, 248, 279, 611
Bst6I CTCTTC 2 cut(s) 515, 609
BstC8I GCNNGC 3 cut(s) 48, 292, 480
BstDEI CTNAG 1 cut(s) 315
BstDSI CCRYGG 1 cut(s) 501
BstENI CCTNNNNNAGG 1 cut(s) 125
BstF5I GGATG 3 cut(s) 130, 211, 263
BstHHI GCGC 1 cut(s) 107
BstKTI GATC 2 cut(s) 193, 200
BstMBI GATC 2 cut(s) 190, 197
BstMWI GCNNNNNNNGC 2 cut(s) 398, 625
BstNI CCWGG 5 cut(s) 110, 122, 248, 279, 611
BstNSI RCATGY 2 cut(s) 294, 638
BstSCI CCNGG 5 cut(s) 108, 120, 246, 277, 609
BstSFI CTRYAG 2 cut(s) 48, 64
BstV1I GCAGC 1 cut(s) 609
BsuRI GGCC 2 cut(s) 46, 246
BtgI CCRYGG 1 cut(s) 501
BtsCI GGATG 3 cut(s) 130, 211, 263
BtsI GCAGTG 3 cut(s) 57, 461, 568
BtsIMutI CAGTG 4 cut(s) 57, 461, 529, 568
BveI ACCTGC 1 cut(s) 383
Cac8I GCNNGC 3 cut(s) 48, 292, 480
CciI TCATGA 1 cut(s) 513
CfoI GCGC 1 cut(s) 107
Cfr13I GGNCC 1 cut(s) 335
Csp6I GTAC 2 cut(s) 61, 670
CviAII CATG 5 cut(s) 291, 424, 514, 635, 673
CviJI RGCY 8 cut(s) 6, 46, 113, 246, 348, 506, 628, 706
CviKI_1 RGCY 8 cut(s) 6, 46, 113, 246, 348, 506, 628, 706
CviQI GTAC 2 cut(s) 61, 670
DdeI CTNAG 1 cut(s) 315
DpnI GATC 2 cut(s) 192, 199
DpnII GATC 2 cut(s) 190, 197
DraI TTTAAA 1 cut(s) 117
DraIII CACNNNGTG 1 cut(s) 332
Eam1104I CTCTTC 2 cut(s) 515, 609
EarI CTCTTC 2 cut(s) 515, 609
Eco130I CCWWGG 1 cut(s) 164
Eco147I AGGCCT 1 cut(s) 46
Eco32I GATATC 1 cut(s) 28
Eco47I GGWCC 1 cut(s) 335
Eco57I CTGAAG 1 cut(s) 269
EcoNI CCTNNNNNAGG 1 cut(s) 125
EcoRII CCWGG 5 cut(s) 108, 120, 246, 277, 609
EcoRV GATATC 1 cut(s) 28
EcoT14I CCWWGG 1 cut(s) 164
ErhI CCWWGG 1 cut(s) 164
FaeI CATG 5 cut(s) 294, 427, 517, 638, 676
FaqI GGGAC 4 cut(s) 321, 434, 504, 617
FatI CATG 5 cut(s) 290, 423, 513, 634, 672
FauI CCCGC 1 cut(s) 593
Fnu4HI GCNGC 1 cut(s) 598
FokI GGATG 3 cut(s) 137, 218, 250
Fsp4HI GCNGC 1 cut(s) 598
FspBI CTAG 1 cut(s) 330
GlaI GCGC 1 cut(s) 106
GluI GCNGC 1 cut(s) 598
HaeIII GGCC 2 cut(s) 46, 246
HhaI GCGC 1 cut(s) 107
Hin1II CATG 5 cut(s) 294, 427, 517, 638, 676
Hin6I GCGC 1 cut(s) 105
HinP1I GCGC 1 cut(s) 105
HincII GTYRAC 2 cut(s) 412, 459
HindII GTYRAC 2 cut(s) 412, 459
HinfI GANTC 3 cut(s) 56, 517, 569
HpaI GTTAAC 1 cut(s) 412
HphI GGTGA 2 cut(s) 564, 707
Hpy166II GTNNAC 4 cut(s) 383, 412, 438, 459
Hpy188I TCNGA 4 cut(s) 11, 100, 238, 549
Hpy188III TCNNGA 2 cut(s) 139, 514
Hpy8I GTNNAC 4 cut(s) 383, 412, 438, 459
HpyAV CCTTC 2 cut(s) 6, 41
HpyCH4V TGCA 7 cut(s) 50, 263, 466, 561, 638, 661, 667
HpyF10VI GCNNNNNNNGC 2 cut(s) 398, 625
HpyF3I CTNAG 1 cut(s) 315
Hsp92II CATG 5 cut(s) 294, 427, 517, 638, 676
HspAI GCGC 1 cut(s) 105
KspAI GTTAAC 1 cut(s) 412
Kzo9I GATC 2 cut(s) 190, 197
LguI GCTCTTC 1 cut(s) 609
Lsp1109I GCAGC 1 cut(s) 609
LweI GCATC 3 cut(s) 272, 328, 465
MaeI CTAG 1 cut(s) 330
MaeIII GTNAC 2 cut(s) 250, 370
MalI GATC 2 cut(s) 192, 199
MboI GATC 2 cut(s) 190, 197
MboII GAAGA 6 cut(s) 169, 221, 502, 539, 578, 626
MluCI AATT 3 cut(s) 213, 351, 442
MlyI GAGTC 1 cut(s) 526
MmeI TCCRAC 1 cut(s) 286
MnlI CCTC 4 cut(s) 36, 121, 426, 597
MseI TTAA 3 cut(s) 90, 116, 411
MslI CAYNNNNRTG 1 cut(s) 677
MspA1I CMGCKG 1 cut(s) 600
MspR9I CCNGG 5 cut(s) 110, 122, 248, 279, 611
Mva1269I GAATGC 1 cut(s) 401
MvaI CCWGG 5 cut(s) 110, 122, 248, 279, 611
MwoI GCNNNNNNNGC 2 cut(s) 398, 625
NdeII GATC 2 cut(s) 190, 197
NlaIII CATG 5 cut(s) 294, 427, 517, 638, 676
NlaIV GGNNCC 1 cut(s) 337
NmuCI GTSAC 2 cut(s) 250, 370
NspI RCATGY 2 cut(s) 294, 638
PaeI GCATGC 1 cut(s) 294
PagI TCATGA 1 cut(s) 513
PceI AGGCCT 1 cut(s) 46
PciSI GCTCTTC 1 cut(s) 609
PctI GAATGC 1 cut(s) 401
PfeI GAWTC 2 cut(s) 56, 569
PflFI GACNNNGTC 1 cut(s) 134
PfoI TCCNGGA 1 cut(s) 277
PkrI GCNGC 1 cut(s) 599
PleI GAGTC 1 cut(s) 525
PpsI GAGTC 1 cut(s) 525
PshBI ATTAAT 1 cut(s) 90
Psp6I CCWGG 5 cut(s) 108, 120, 246, 277, 609
PspGI CCWGG 5 cut(s) 108, 120, 246, 277, 609
PspN4I GGNNCC 1 cut(s) 337
PspPI GGNCC 1 cut(s) 335
PstI CTGCAG 1 cut(s) 52
PsyI GACNNNGTC 1 cut(s) 134
RsaI GTAC 2 cut(s) 62, 671
RsaNI GTAC 2 cut(s) 61, 670
RseI CAYNNNNRTG 1 cut(s) 677
SapI GCTCTTC 1 cut(s) 609
SaqAI TTAA 3 cut(s) 90, 116, 411
SatI GCNGC 1 cut(s) 598
Sau3AI GATC 2 cut(s) 190, 197
Sau96I GGNCC 1 cut(s) 335
SchI GAGTC 1 cut(s) 526
ScrFI CCNGG 5 cut(s) 110, 122, 248, 279, 611
SetI ASST 8 cut(s) 123, 331, 350, 397, 437, 458, 612, 651
SfaNI GCATC 3 cut(s) 272, 328, 465
SfcI CTRYAG 2 cut(s) 48, 64
SinI GGWCC 1 cut(s) 335
SmiMI CAYNNNNRTG 1 cut(s) 677
SphI GCATGC 1 cut(s) 294
Sse9I AATT 3 cut(s) 213, 351, 442
SseBI AGGCCT 1 cut(s) 46
SsiI CCGC 3 cut(s) 575, 595, 600
SspMI CTAG 1 cut(s) 330
StuI AGGCCT 1 cut(s) 46
StyD4I CCNGG 5 cut(s) 108, 120, 246, 277, 609
StyI CCWWGG 1 cut(s) 164
TaqI TCGA 2 cut(s) 138, 322
TasI AATT 3 cut(s) 213, 351, 442
TatI WGTACW 1 cut(s) 669
TfiI GAWTC 2 cut(s) 56, 569
Tru1I TTAA 3 cut(s) 90, 116, 411
Tru9I TTAA 3 cut(s) 90, 116, 411
TscAI CASTG 4 cut(s) 57, 468, 529, 568
TseFI GTSAC 2 cut(s) 250, 370
TseI GCWGC 1 cut(s) 597
Tsp45I GTSAC 2 cut(s) 250, 370
TspDTI ATGAA 4 cut(s) 170, 222, 394, 502
TspGWI ACGGA 2 cut(s) 218, 490
TspRI CASTG 4 cut(s) 57, 468, 529, 568
Tth111I GACNNNGTC 1 cut(s) 134
VpaK11BI GGWCC 1 cut(s) 335
VspI ATTAAT 1 cut(s) 90
XagI CCTNNNNNAGG 1 cut(s) 125
XapI RAATTY 1 cut(s) 213
XceI RCATGY 2 cut(s) 294, 638
XspI CTAG 1 cut(s) 330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.