Rh7BG209300

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0001740
Physical Location & Seq
Reverse (-)
17652720 .. 17656749
4030 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG209300.1

Sequence Viewer

Length: 768 bp
ATGGCGCAGGGTCTGAAGCTGCCTATCATAGACTTGTCCTCACCGGACCGCACCTCCTCCGCCAGTTTAATCCGTCAGGCTTGCATAGAGTGTGGTTTCTTTTACTTGGTGAATCATGGTGTTGAGGAAGAGTTACTTGGCCGAGTGTTTGATCAGAGCAGTAAGTTTTTTTCACTTCCTTTGGAAGTTAAATCCAAATTGGCGCGCAAACAAAACAGAGGCTACACAGCGCTCTTTGCTGAGAACCTTGACCCCACTGCCAGCTCCAAAGGTGATTCAAAAGAGAGTTTTTACATTGGCCCTTTAGAAGACGCAACTCAAAATAAGTTGAATCAATGGCCTTCAGAAGATATCCTTCCATCCTGGAGACCTACAATGGAATCCTACTATAAAAAAGTTCTTTCAGCTGGGAAAAGATTATTATCTCTGATTGCCCTGGCTTTGAACCTGGATGAGGATTTTTTTGAGAAAGTGGGGGGTCTGAATAAACACACAGCACTTCTTCGCCTTTTGCACTACCCAGGTCAACTGGGATCATCTGATGAAGAAGTACTTGGTGCTTCTGCTCATTCAGATTATGGAACAGTCACACTCCTGGCATCCAATGGTGTCCCAGGACTTCAGGTGGCTCTCTTCTTAGACCCCAATGAAGACTCTCTGGTAGAATGCCTGGCAAGTTGTTGCAATGAATCATTGCCGCCAAGATTTCCTCCCATCCGCAGCGGGGACTACCTGAAAGAGCGGTTTAGGCTTACTTATGGCTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

255

Amino Acids

28.17

Weight (kDa)

5.34

Isoelectric Point (pI)

52.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 7 - 116 1.6e-31 non-haem dioxygenase in morphine synthesis N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 742
AccII CGCG 1 cut(s) 205
AciI CCGC 6 cut(s) 49, 60, 698, 718, 723, 742
AclWI GGATC 1 cut(s) 541
AcoI YGGCCR 1 cut(s) 139
AcuI CTGAAG 3 cut(s) 35, 327, 605
AfaI GTAC 1 cut(s) 552
AfeI AGCGCT 1 cut(s) 231
AfiI CCNNNNNNNGG 2 cut(s) 454, 724
AgsI TTSAA 3 cut(s) 279, 331, 445
AjnI CCWGG 7 cut(s) 362, 435, 447, 520, 594, 613, 669
AjuI GAANNNNNNNTTGG 4 cut(s) 120, 152, 537, 569
AluBI AGCT 3 cut(s) 19, 264, 407
AluI AGCT 3 cut(s) 19, 264, 407
Alw26I GTCTC 1 cut(s) 361
AlwI GGATC 1 cut(s) 541
AlwNI CAGNNNCTG 1 cut(s) 13
Aor51HI AGCGCT 1 cut(s) 231
AoxI GGCC 3 cut(s) 139, 298, 338
ApeKI GCWGC 2 cut(s) 19, 720
AspLEI GCGC 4 cut(s) 7, 205, 207, 232
AspS9I GGNCC 2 cut(s) 46, 299
AsuHPI GGTGA 3 cut(s) 33, 121, 284
AvaII GGWCC 1 cut(s) 46
BbsI GAAGAC 2 cut(s) 315, 657
BbvI GCAGC 2 cut(s) 6, 732
BccI CCATC 2 cut(s) 367, 722
BciT130I CCWGG 7 cut(s) 364, 437, 449, 522, 596, 615, 671
BclI TGATCA 1 cut(s) 151
BcoDI GTCTC 1 cut(s) 361
BfoI RGCGCY 1 cut(s) 233
BisI GCNGC 3 cut(s) 20, 698, 721
BlsI GCNGC 3 cut(s) 21, 699, 722
BmcAI AGTACT 1 cut(s) 552
Bme1390I CCNGG 7 cut(s) 364, 437, 449, 522, 596, 615, 671
Bme18I GGWCC 1 cut(s) 46
BmgT120I GGNCC 2 cut(s) 46, 299
BmrFI CCNGG 7 cut(s) 364, 437, 449, 522, 596, 615, 671
BmrI ACTGGG 1 cut(s) 539
BmsI GCATC 1 cut(s) 608
BmuI ACTGGG 1 cut(s) 539
BpiI GAAGAC 2 cut(s) 315, 657
BpmI CTGGAG 1 cut(s) 385
BsaBI GATNNNNATC 1 cut(s) 421
BsaI GGTCTC 1 cut(s) 361
BsaJI CCNNGG 3 cut(s) 435, 520, 613
BsaWI WCCGGW 1 cut(s) 43
BsaXI ACNNNNNCTCC 2 cut(s) 38, 68
Bsc4I CCNNNNNNNGG 2 cut(s) 454, 724
Bse1I ACTGG 2 cut(s) 63, 534
Bse3DI GCAATG 2 cut(s) 691, 692
Bse8I GATNNNNATC 1 cut(s) 421
BseBI CCWGG 7 cut(s) 364, 437, 449, 522, 596, 615, 671
BseDI CCNNGG 3 cut(s) 435, 520, 613
BseGI GGATG 4 cut(s) 359, 457, 599, 714
BseJI GATNNNNATC 1 cut(s) 421
BseLI CCNNNNNNNGG 2 cut(s) 454, 724
BseMI GCAATG 2 cut(s) 691, 692
BseMII CTCAG 1 cut(s) 231
BseNI ACTGG 2 cut(s) 63, 534
BsePI GCGCGC 1 cut(s) 203
BseRI GAGGAG 1 cut(s) 46
BseXI GCAGC 2 cut(s) 6, 732
BseYI CCCAGC 1 cut(s) 407
Bsh1236I CGCG 1 cut(s) 205
BshFI GGCC 3 cut(s) 141, 300, 340
BsiSI CCGG 1 cut(s) 44
BslFI GGGAC 2 cut(s) 596, 740
BslI CCNNNNNNNGG 2 cut(s) 454, 724
BsmAI GTCTC 1 cut(s) 361
BsmFI GGGAC 2 cut(s) 596, 740
BsmI GAATGC 1 cut(s) 671
BsnI GGCC 3 cut(s) 141, 300, 340
Bso31I GGTCTC 1 cut(s) 361
Bsp143I GATC 2 cut(s) 151, 533
BspACI CCGC 6 cut(s) 49, 60, 698, 718, 723, 742
BspANI GGCC 3 cut(s) 141, 300, 340
BspCNI CTCAG 1 cut(s) 232
BspFNI CGCG 1 cut(s) 205
BspPI GGATC 1 cut(s) 541
BspTNI GGTCTC 1 cut(s) 361
BsrBI CCGCTC 1 cut(s) 742
BsrDI GCAATG 2 cut(s) 691, 692
BsrI ACTGG 2 cut(s) 63, 534
BssECI CCNNGG 3 cut(s) 435, 520, 613
BssHII GCGCGC 1 cut(s) 203
BssMI GATC 2 cut(s) 151, 533
Bst2UI CCWGG 7 cut(s) 364, 437, 449, 522, 596, 615, 671
Bst4CI ACNGT 1 cut(s) 586
Bst6I CTCTTC 2 cut(s) 123, 638
BstC8I GCNNGC 3 cut(s) 82, 205, 262
BstDEI CTNAG 2 cut(s) 240, 637
BstENI CCTNNNNNAGG 1 cut(s) 452
BstF5I GGATG 4 cut(s) 359, 457, 599, 714
BstFNI CGCG 1 cut(s) 205
BstH2I RGCGCY 1 cut(s) 233
BstHHI GCGC 4 cut(s) 7, 205, 207, 232
BstKTI GATC 2 cut(s) 154, 536
BstMAI GTCTC 1 cut(s) 361
BstMBI GATC 2 cut(s) 151, 533
BstMWI GCNNNNNNNGC 2 cut(s) 236, 748
BstNI CCWGG 7 cut(s) 364, 437, 449, 522, 596, 615, 671
BstSCI CCNGG 7 cut(s) 362, 435, 447, 520, 594, 613, 669
BstUI CGCG 1 cut(s) 205
BstV1I GCAGC 2 cut(s) 6, 732
BstV2I GAAGAC 2 cut(s) 315, 657
BsuRI GGCC 3 cut(s) 141, 300, 340
BtsCI GGATG 4 cut(s) 359, 457, 599, 714
BtsI GCAGTG 1 cut(s) 255
BtsIMutI CAGTG 1 cut(s) 255
Cac8I GCNNGC 3 cut(s) 82, 205, 262
CaiI CAGNNNCTG 1 cut(s) 13
CfoI GCGC 4 cut(s) 7, 205, 207, 232
Cfr13I GGNCC 2 cut(s) 46, 299
CpoI CGGWCCG 1 cut(s) 46
CseI GACGC 1 cut(s) 320
Csp6I GTAC 1 cut(s) 551
CspI CGGWCCG 1 cut(s) 46
CviAII CATG 1 cut(s) 116
CviQI GTAC 1 cut(s) 551
DdeI CTNAG 2 cut(s) 240, 637
DpnI GATC 2 cut(s) 153, 535
DpnII GATC 2 cut(s) 151, 533
EaeI YGGCCR 1 cut(s) 139
Eam1104I CTCTTC 2 cut(s) 123, 638
EarI CTCTTC 2 cut(s) 123, 638
EciI GGCGGA 1 cut(s) 49
Eco31I GGTCTC 1 cut(s) 361
Eco32I GATATC 1 cut(s) 352
Eco47I GGWCC 1 cut(s) 46
Eco47III AGCGCT 1 cut(s) 231
Eco57I CTGAAG 3 cut(s) 35, 327, 605
EcoNI CCTNNNNNAGG 1 cut(s) 452
EcoRII CCWGG 7 cut(s) 362, 435, 447, 520, 594, 613, 669
EcoRV GATATC 1 cut(s) 352
FaeI CATG 1 cut(s) 119
FaiI YATR 7 cut(s) 29, 86, 117, 390, 579, 759, 766
FalI AAGNNNNNCTT 6 cut(s) 120, 152, 339, 371, 537, 569
FaqI GGGAC 2 cut(s) 596, 740
FatI CATG 1 cut(s) 115
FauI CCCGC 1 cut(s) 716
FbaI TGATCA 1 cut(s) 151
Fnu4HI GCNGC 3 cut(s) 20, 698, 721
FokI GGATG 4 cut(s) 346, 464, 586, 701
Fsp4HI GCNGC 3 cut(s) 20, 698, 721
GlaI GCGC 4 cut(s) 6, 204, 206, 231
GluI GCNGC 3 cut(s) 20, 698, 721
GsaI CCCAGC 1 cut(s) 411
GsuI CTGGAG 1 cut(s) 385
HaeII RGCGCY 1 cut(s) 233
HaeIII GGCC 3 cut(s) 141, 300, 340
HapII CCGG 1 cut(s) 44
HgaI GACGC 1 cut(s) 320
HhaI GCGC 4 cut(s) 7, 205, 207, 232
Hin1II CATG 1 cut(s) 119
Hin6I GCGC 4 cut(s) 5, 203, 205, 230
HinP1I GCGC 4 cut(s) 5, 203, 205, 230
HincII GTYRAC 1 cut(s) 527
HindII GTYRAC 1 cut(s) 527
HinfI GANTC 6 cut(s) 112, 275, 331, 380, 653, 689
HpaII CCGG 1 cut(s) 44
HphI GGTGA 3 cut(s) 33, 121, 284
Hpy166II GTNNAC 1 cut(s) 527
Hpy188I TCNGA 7 cut(s) 15, 156, 346, 429, 483, 541, 574
Hpy8I GTNNAC 1 cut(s) 527
HpyAV CCTTC 2 cut(s) 351, 365
HpyCH4III ACNGT 1 cut(s) 586
HpyCH4V TGCA 3 cut(s) 84, 514, 684
HpyF10VI GCNNNNNNNGC 2 cut(s) 236, 748
HpyF3I CTNAG 2 cut(s) 240, 637
Hsp92II CATG 1 cut(s) 119
HspAI GCGC 4 cut(s) 5, 203, 205, 230
Ksp22I TGATCA 1 cut(s) 151
Kzo9I GATC 2 cut(s) 151, 533
LmnI GCTCC 1 cut(s) 269
Lsp1109I GCAGC 2 cut(s) 6, 732
LweI GCATC 1 cut(s) 608
MaeIII GTNAC 2 cut(s) 132, 586
MalI GATC 2 cut(s) 153, 535
MbiI CCGCTC 1 cut(s) 742
MboI GATC 2 cut(s) 151, 533
MboII GAAGA 7 cut(s) 140, 320, 359, 494, 557, 625, 662
MluCI AATT 1 cut(s) 197
MlyI GAGTC 1 cut(s) 647
MnlI CCTC 7 cut(s) 49, 64, 67, 118, 212, 448, 720
MseI TTAA 2 cut(s) 68, 189
MspA1I CMGCKG 2 cut(s) 407, 723
MspI CCGG 1 cut(s) 44
MspR9I CCNGG 7 cut(s) 364, 437, 449, 522, 596, 615, 671
Mva1269I GAATGC 1 cut(s) 671
MvaI CCWGG 7 cut(s) 364, 437, 449, 522, 596, 615, 671
MvnI CGCG 1 cut(s) 205
MwoI GCNNNNNNNGC 2 cut(s) 236, 748
NdeII GATC 2 cut(s) 151, 533
NlaIII CATG 1 cut(s) 119
NmeAIII GCCGAG 1 cut(s) 167
NmuCI GTSAC 1 cut(s) 586
PauI GCGCGC 1 cut(s) 203
PctI GAATGC 1 cut(s) 671
PfeI GAWTC 5 cut(s) 112, 275, 331, 380, 689
PfoI TCCNGGA 1 cut(s) 362
PkrI GCNGC 3 cut(s) 21, 699, 722
PleI GAGTC 1 cut(s) 647
PpsI GAGTC 1 cut(s) 647
Psp6I CCWGG 7 cut(s) 362, 435, 447, 520, 594, 613, 669
PspFI CCCAGC 1 cut(s) 407
PspGI CCWGG 7 cut(s) 362, 435, 447, 520, 594, 613, 669
PspPI GGNCC 2 cut(s) 46, 299
PstNI CAGNNNCTG 1 cut(s) 13
PteI GCGCGC 1 cut(s) 203
PvuII CAGCTG 1 cut(s) 407
RsaI GTAC 1 cut(s) 552
RsaNI GTAC 1 cut(s) 551
Rsr2I CGGWCCG 1 cut(s) 46
RsrII CGGWCCG 1 cut(s) 46
SaqAI TTAA 2 cut(s) 68, 189
SatI GCNGC 3 cut(s) 20, 698, 721
Sau3AI GATC 2 cut(s) 151, 533
Sau96I GGNCC 2 cut(s) 46, 299
ScaI AGTACT 1 cut(s) 552
SchI GAGTC 1 cut(s) 647
ScrFI CCNGG 7 cut(s) 364, 437, 449, 522, 596, 615, 671
SfaNI GCATC 1 cut(s) 608
SinI GGWCC 1 cut(s) 46
Sse9I AATT 1 cut(s) 197
SsiI CCGC 6 cut(s) 49, 60, 698, 718, 723, 742
StyD4I CCNGG 7 cut(s) 362, 435, 447, 520, 594, 613, 669
TaaI ACNGT 1 cut(s) 586
TasI AATT 1 cut(s) 197
TatI WGTACW 1 cut(s) 550
TauI GCSGC 1 cut(s) 700
TfiI GAWTC 5 cut(s) 112, 275, 331, 380, 689
Tru1I TTAA 2 cut(s) 68, 189
Tru9I TTAA 2 cut(s) 68, 189
TscAI CASTG 1 cut(s) 262
TseFI GTSAC 1 cut(s) 586
TseI GCWGC 2 cut(s) 19, 720
Tsp45I GTSAC 1 cut(s) 586
TspDTI ATGAA 3 cut(s) 558, 663, 702
TspGWI ACGGA 1 cut(s) 62
TspRI CASTG 1 cut(s) 262
VpaK11BI GGWCC 1 cut(s) 46
XagI CCTNNNNNAGG 1 cut(s) 452
ZrmI AGTACT 1 cut(s) 552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.