Rroxscaffold_3G00253340

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001740
Physical Location & Seq
Forward (+)
47370909 .. 47376335
5427 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00253340.1

Sequence Viewer

Length: 1053 bp
ATGGCGGAGGGTCTGAAGCTGCCTATCATAGACTTGTCCTCACCGGACCGCACCTCCTCCGCCAGTTTAATTCGTCAGGCTTGCATAGAGTGTGGTTTCTTTTACTTGGTGAATCATGGTGTTGAGGAAGAGTTACTTGGCCGAGTGTTTGATCAGAGCAGTAAGTTTTTTTCACTTCCTTTGGAAGTTAAGTCCAAATTGGGGCGCAAACAAAACAGAGGCTACACAGCGCTCTTTGCTGAGAACCTTGACCCCACTGCCAGCTCCAAAGGTGATTCAAAAGAGAGTTTTTACATTGGCCCTTTAGAAGACGCAACTCAAAATAAGTTGAATCAATGGCCTTCAGAAGATATCCTTCCATCCTGGAGACCTACGATGGAATCCTACTATAAAAAAGTTCTTTCCTCTGGGAAAAGATTATTATCTCTGATTGCCCTGGCTTTGAACCTGGATGAGGATTTTTTTGAGAAAGTGGGGGGTCTGAATAAACACACAGCACTTCTTCGTCTTTTGCACTACCCAGGCCAACTGGGATCATCTGATGAAGAAGTACTTGGTGCTTCTGCTCATTCAGATTATGGAACAGTCACACTCCTGGCATCCAATGGTGTCCCAGGACTTCAGGTGGCTTTGTTCTTAGACCCCAATGAAGACGCTATGGTAGAATGCCTGGCAAGTTGTTGCAATGAATCATTGCCGCCAAGCCGTGACGGTGCCGCGGGACTTGCCGGACGGAGGCCAGGGGTTGATCTCGACCTTCTTCTTGCTGGTGGAGTCGTCGGCTATCAATTTGCGGTCGTTGCCCGAAGCTTGAAGTTGCGGGTGAGGTCGCTGCCCGGTACCTTCAACCTTGATCTCCTTCGACTTCGATCATTGCCTAGAAGCGATGCGCGGGATGCCGCTGGCCTCCGTCCGGCAAGATTCGAGCCACCGTCGGCGCACGAACTCGGCTGCAGCGTCGCCATCTGCACTTTAGCGACGGTGCGGACATTTCCTCCCATCCGCAGCGGGGACTACCTGAAAGAGCGGTTTAGGCTTACATATGGCTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

350

Amino Acids

38.13

Weight (kDa)

6.26

Isoelectric Point (pI)

54.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 7 - 116 1.4e-30 non-haem dioxygenase in morphine synthesis N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 839
AccB1I GGYRCC 2 cut(s) 713, 839
AccBSI CCGCTC 1 cut(s) 1027
AccII CGCG 2 cut(s) 719, 892
AclWI GGATC 1 cut(s) 541
AcoI YGGCCR 1 cut(s) 139
AcuI CTGAAG 3 cut(s) 35, 327, 605
AfaI GTAC 2 cut(s) 552, 841
AfeI AGCGCT 1 cut(s) 231
AfiI CCNNNNNNNGG 5 cut(s) 201, 454, 735, 913, 1009
AgsI TTSAA 5 cut(s) 279, 331, 445, 814, 847
AjnI CCWGG 8 cut(s) 362, 435, 447, 520, 594, 613, 669, 739
AjuI GAANNNNNNNTTGG 4 cut(s) 120, 152, 537, 569
AluBI AGCT 3 cut(s) 19, 264, 810
AluI AGCT 3 cut(s) 19, 264, 810
Alw26I GTCTC 1 cut(s) 361
AlwI GGATC 1 cut(s) 541
Aor51HI AGCGCT 1 cut(s) 231
AoxI GGCC 6 cut(s) 139, 298, 338, 523, 737, 904
ApeKI GCWGC 5 cut(s) 19, 832, 951, 954, 1005
Asp718I GGTACC 1 cut(s) 839
AspLEI GCGC 4 cut(s) 207, 232, 892, 940
AspS9I GGNCC 2 cut(s) 46, 299
AsuC2I CCSGG 1 cut(s) 837
AsuHPI GGTGA 4 cut(s) 33, 121, 284, 835
AvaII GGWCC 1 cut(s) 46
BanI GGYRCC 2 cut(s) 713, 839
BbsI GAAGAC 2 cut(s) 315, 657
BbvI GCAGC 5 cut(s) 6, 819, 938, 966, 1017
BccI CCATC 4 cut(s) 367, 370, 971, 1007
BceAI ACGGC 1 cut(s) 690
BciT130I CCWGG 8 cut(s) 364, 437, 449, 522, 596, 615, 671, 741
BclI TGATCA 1 cut(s) 151
BcnI CCSGG 1 cut(s) 837
BcoDI GTCTC 1 cut(s) 361
BfaI CTAG 1 cut(s) 879
BfmI CTRYAG 1 cut(s) 952
BfoI RGCGCY 1 cut(s) 233
BisI GCNGC 8 cut(s) 20, 698, 717, 833, 900, 952, 955, 1006
BlsI GCNGC 8 cut(s) 21, 699, 718, 834, 901, 953, 956, 1007
BmcAI AGTACT 1 cut(s) 552
Bme1390I CCNGG 9 cut(s) 364, 437, 449, 522, 596, 615, 671, 741, 837
Bme18I GGWCC 1 cut(s) 46
BmgT120I GGNCC 2 cut(s) 46, 299
BmiI GGNNCC 2 cut(s) 715, 841
BmrFI CCNGG 9 cut(s) 364, 437, 449, 522, 596, 615, 671, 741, 837
BmrI ACTGGG 1 cut(s) 539
BmsI GCATC 3 cut(s) 608, 877, 886
BmuI ACTGGG 1 cut(s) 539
BpiI GAAGAC 2 cut(s) 315, 657
BpmI CTGGAG 1 cut(s) 385
BpuMI CCSGG 1 cut(s) 837
BsaBI GATNNNNATC 1 cut(s) 421
BsaI GGTCTC 1 cut(s) 361
BsaJI CCNNGG 5 cut(s) 435, 520, 613, 717, 740
BsaWI WCCGGW 1 cut(s) 43
BsaXI ACNNNNNCTCC 4 cut(s) 38, 68, 979, 1009
Bsc4I CCNNNNNNNGG 5 cut(s) 201, 454, 735, 913, 1009
Bse1I ACTGG 2 cut(s) 63, 534
Bse3DI GCAATG 3 cut(s) 691, 692, 872
Bse8I GATNNNNATC 1 cut(s) 421
BseBI CCWGG 8 cut(s) 364, 437, 449, 522, 596, 615, 671, 741
BseDI CCNNGG 5 cut(s) 435, 520, 613, 717, 740
BseGI GGATG 5 cut(s) 359, 457, 599, 901, 999
BseJI GATNNNNATC 1 cut(s) 421
BseLI CCNNNNNNNGG 5 cut(s) 201, 454, 735, 913, 1009
BseMI GCAATG 3 cut(s) 691, 692, 872
BseMII CTCAG 1 cut(s) 231
BseNI ACTGG 2 cut(s) 63, 534
BseRI GAGGAG 1 cut(s) 46
BseXI GCAGC 5 cut(s) 6, 819, 938, 966, 1017
BsgI GTGCAG 1 cut(s) 952
Bsh1236I CGCG 2 cut(s) 719, 892
Bsh1285I CGRYCG 1 cut(s) 798
BshFI GGCC 6 cut(s) 141, 300, 340, 525, 739, 906
BshNI GGYRCC 2 cut(s) 713, 839
BsiEI CGRYCG 1 cut(s) 798
BsiSI CCGG 4 cut(s) 44, 729, 837, 914
BslFI GGGAC 3 cut(s) 596, 735, 1025
BslI CCNNNNNNNGG 5 cut(s) 201, 454, 735, 913, 1009
BsmAI GTCTC 1 cut(s) 361
BsmFI GGGAC 3 cut(s) 596, 735, 1025
BsmI GAATGC 1 cut(s) 671
BsnI GGCC 6 cut(s) 141, 300, 340, 525, 739, 906
Bso31I GGTCTC 1 cut(s) 361
Bsp143I GATC 5 cut(s) 151, 533, 748, 853, 869
BspANI GGCC 6 cut(s) 141, 300, 340, 525, 739, 906
BspCNI CTCAG 1 cut(s) 232
BspFNI CGCG 2 cut(s) 719, 892
BspLI GGNNCC 2 cut(s) 715, 841
BspMAI CTGCAG 1 cut(s) 956
BspPI GGATC 1 cut(s) 541
BspT107I GGYRCC 2 cut(s) 713, 839
BspTNI GGTCTC 1 cut(s) 361
BsrBI CCGCTC 1 cut(s) 1027
BsrDI GCAATG 3 cut(s) 691, 692, 872
BsrI ACTGG 2 cut(s) 63, 534
BssECI CCNNGG 5 cut(s) 435, 520, 613, 717, 740
BssMI GATC 5 cut(s) 151, 533, 748, 853, 869
Bst2UI CCWGG 8 cut(s) 364, 437, 449, 522, 596, 615, 671, 741
Bst4CI ACNGT 4 cut(s) 586, 713, 933, 982
Bst6I CTCTTC 1 cut(s) 123
BstC8I GCNNGC 3 cut(s) 82, 262, 904
BstDEI CTNAG 2 cut(s) 240, 637
BstDSI CCRYGG 1 cut(s) 717
BstENI CCTNNNNNAGG 1 cut(s) 452
BstF5I GGATG 5 cut(s) 359, 457, 599, 901, 999
BstFNI CGCG 2 cut(s) 719, 892
BstH2I RGCGCY 1 cut(s) 233
BstHHI GCGC 4 cut(s) 207, 232, 892, 940
BstKTI GATC 5 cut(s) 154, 536, 751, 856, 872
BstMAI GTCTC 1 cut(s) 361
BstMBI GATC 5 cut(s) 151, 533, 748, 853, 869
BstMCI CGRYCG 1 cut(s) 798
BstMWI GCNNNNNNNGC 5 cut(s) 236, 725, 800, 896, 1033
BstNI CCWGG 8 cut(s) 364, 437, 449, 522, 596, 615, 671, 741
BstSCI CCNGG 9 cut(s) 362, 435, 447, 520, 594, 613, 669, 739, 835
BstSFI CTRYAG 1 cut(s) 952
BstUI CGCG 2 cut(s) 719, 892
BstV1I GCAGC 5 cut(s) 6, 819, 938, 966, 1017
BstV2I GAAGAC 2 cut(s) 315, 657
BsuRI GGCC 6 cut(s) 141, 300, 340, 525, 739, 906
BtgI CCRYGG 1 cut(s) 717
BtgZI GCGATG 1 cut(s) 900
BtsCI GGATG 5 cut(s) 359, 457, 599, 901, 999
BtsI GCAGTG 1 cut(s) 255
BtsIMutI CAGTG 1 cut(s) 255
Cac8I GCNNGC 3 cut(s) 82, 262, 904
CfoI GCGC 4 cut(s) 207, 232, 892, 940
Cfr13I GGNCC 2 cut(s) 46, 299
Cfr42I CCGCGG 1 cut(s) 720
CpoI CGGWCCG 1 cut(s) 46
CseI GACGC 3 cut(s) 320, 662, 946
Csp6I GTAC 2 cut(s) 551, 840
CspI CGGWCCG 1 cut(s) 46
CviAII CATG 1 cut(s) 116
CviQI GTAC 2 cut(s) 551, 840
DdeI CTNAG 2 cut(s) 240, 637
DpnI GATC 5 cut(s) 153, 535, 750, 855, 871
DpnII GATC 5 cut(s) 151, 533, 748, 853, 869
EaeI YGGCCR 1 cut(s) 139
Eam1104I CTCTTC 1 cut(s) 123
EarI CTCTTC 1 cut(s) 123
EciI GGCGGA 2 cut(s) 20, 49
Eco31I GGTCTC 1 cut(s) 361
Eco32I GATATC 1 cut(s) 352
Eco47I GGWCC 1 cut(s) 46
Eco47III AGCGCT 1 cut(s) 231
Eco57I CTGAAG 3 cut(s) 35, 327, 605
EcoNI CCTNNNNNAGG 1 cut(s) 452
EcoRII CCWGG 8 cut(s) 362, 435, 447, 520, 594, 613, 669, 739
EcoRV GATATC 1 cut(s) 352
FaeI CATG 1 cut(s) 119
FaiI YATR 9 cut(s) 29, 86, 117, 390, 579, 659, 1042, 1044, 1051
FalI AAGNNNNNCTT 6 cut(s) 120, 152, 339, 371, 537, 569
FaqI GGGAC 3 cut(s) 596, 735, 1025
FatI CATG 1 cut(s) 115
FauI CCCGC 4 cut(s) 712, 813, 885, 1001
FauNDI CATATG 1 cut(s) 1042
FbaI TGATCA 1 cut(s) 151
Fnu4HI GCNGC 8 cut(s) 20, 698, 717, 833, 900, 952, 955, 1006
FokI GGATG 5 cut(s) 346, 464, 586, 908, 986
Fsp4HI GCNGC 8 cut(s) 20, 698, 717, 833, 900, 952, 955, 1006
FspBI CTAG 1 cut(s) 879
GlaI GCGC 4 cut(s) 206, 231, 891, 939
GluI GCNGC 8 cut(s) 20, 698, 717, 833, 900, 952, 955, 1006
GsuI CTGGAG 1 cut(s) 385
HaeII RGCGCY 1 cut(s) 233
HaeIII GGCC 6 cut(s) 141, 300, 340, 525, 739, 906
HapII CCGG 4 cut(s) 44, 729, 837, 914
HgaI GACGC 3 cut(s) 320, 662, 946
HhaI GCGC 4 cut(s) 207, 232, 892, 940
Hin1II CATG 1 cut(s) 119
Hin6I GCGC 4 cut(s) 205, 230, 890, 938
HinP1I GCGC 4 cut(s) 205, 230, 890, 938
HindIII AAGCTT 1 cut(s) 808
HinfI GANTC 7 cut(s) 112, 275, 331, 380, 689, 774, 921
HpaII CCGG 4 cut(s) 44, 729, 837, 914
HphI GGTGA 4 cut(s) 33, 121, 284, 835
Hpy188I TCNGA 7 cut(s) 15, 156, 346, 429, 483, 541, 574
Hpy188III TCNNGA 1 cut(s) 752
Hpy99I CGWCG 4 cut(s) 782, 937, 962, 982
HpyAV CCTTC 5 cut(s) 351, 365, 767, 853, 869
HpyCH4III ACNGT 4 cut(s) 586, 713, 933, 982
HpyCH4V TGCA 5 cut(s) 84, 514, 684, 954, 969
HpyF10VI GCNNNNNNNGC 5 cut(s) 236, 725, 800, 896, 1033
HpyF3I CTNAG 2 cut(s) 240, 637
Hsp92II CATG 1 cut(s) 119
HspAI GCGC 4 cut(s) 205, 230, 890, 938
KpnI GGTACC 1 cut(s) 843
Ksp22I TGATCA 1 cut(s) 151
KspI CCGCGG 1 cut(s) 720
Kzo9I GATC 5 cut(s) 151, 533, 748, 853, 869
LmnI GCTCC 1 cut(s) 269
Lsp1109I GCAGC 5 cut(s) 6, 819, 938, 966, 1017
LweI GCATC 3 cut(s) 608, 877, 886
MaeI CTAG 1 cut(s) 879
MaeIII GTNAC 3 cut(s) 132, 586, 707
MalI GATC 5 cut(s) 153, 535, 750, 855, 871
MbiI CCGCTC 1 cut(s) 1027
MboI GATC 5 cut(s) 151, 533, 748, 853, 869
MboII GAAGA 7 cut(s) 140, 320, 359, 494, 557, 662, 752
MluCI AATT 3 cut(s) 69, 197, 788
MlyI GAGTC 1 cut(s) 783
MseI TTAA 2 cut(s) 68, 189
MspA1I CMGCKG 3 cut(s) 719, 902, 1008
MspI CCGG 4 cut(s) 44, 729, 837, 914
MspR9I CCNGG 9 cut(s) 364, 437, 449, 522, 596, 615, 671, 741, 837
Mva1269I GAATGC 1 cut(s) 671
MvaI CCWGG 8 cut(s) 364, 437, 449, 522, 596, 615, 671, 741
MvnI CGCG 2 cut(s) 719, 892
MwoI GCNNNNNNNGC 5 cut(s) 236, 725, 800, 896, 1033
NciI CCSGG 1 cut(s) 837
NdeI CATATG 1 cut(s) 1042
NdeII GATC 5 cut(s) 151, 533, 748, 853, 869
NlaIII CATG 1 cut(s) 119
NlaIV GGNNCC 2 cut(s) 715, 841
NmeAIII GCCGAG 2 cut(s) 167, 927
NmuCI GTSAC 2 cut(s) 586, 707
PcsI WCGNNNNNNNCGW 1 cut(s) 954
PctI GAATGC 1 cut(s) 671
PfeI GAWTC 6 cut(s) 112, 275, 331, 380, 689, 921
PfoI TCCNGGA 1 cut(s) 362
PkrI GCNGC 8 cut(s) 21, 699, 718, 834, 901, 953, 956, 1007
PleI GAGTC 1 cut(s) 782
PpsI GAGTC 1 cut(s) 782
Psp6I CCWGG 8 cut(s) 362, 435, 447, 520, 594, 613, 669, 739
PspGI CCWGG 8 cut(s) 362, 435, 447, 520, 594, 613, 669, 739
PspN4I GGNNCC 2 cut(s) 715, 841
PspPI GGNCC 2 cut(s) 46, 299
PstI CTGCAG 1 cut(s) 956
RsaI GTAC 2 cut(s) 552, 841
RsaNI GTAC 2 cut(s) 551, 840
Rsr2I CGGWCCG 1 cut(s) 46
RsrII CGGWCCG 1 cut(s) 46
SacII CCGCGG 1 cut(s) 720
SaqAI TTAA 2 cut(s) 68, 189
SatI GCNGC 8 cut(s) 20, 698, 717, 833, 900, 952, 955, 1006
Sau3AI GATC 5 cut(s) 151, 533, 748, 853, 869
Sau96I GGNCC 2 cut(s) 46, 299
ScaI AGTACT 1 cut(s) 552
SchI GAGTC 1 cut(s) 783
ScrFI CCNGG 9 cut(s) 364, 437, 449, 522, 596, 615, 671, 741, 837
SfaNI GCATC 3 cut(s) 608, 877, 886
SfcI CTRYAG 1 cut(s) 952
Sfr303I CCGCGG 1 cut(s) 720
SgrBI CCGCGG 1 cut(s) 720
SinI GGWCC 1 cut(s) 46
Sse9I AATT 3 cut(s) 69, 197, 788
SspMI CTAG 1 cut(s) 879
StyD4I CCNGG 9 cut(s) 362, 435, 447, 520, 594, 613, 669, 739, 835
TaaI ACNGT 4 cut(s) 586, 713, 933, 982
TaqI TCGA 4 cut(s) 753, 862, 868, 924
TasI AATT 3 cut(s) 69, 197, 788
TatI WGTACW 1 cut(s) 550
TauI GCSGC 3 cut(s) 700, 719, 902
TfiI GAWTC 6 cut(s) 112, 275, 331, 380, 689, 921
Tru1I TTAA 2 cut(s) 68, 189
Tru9I TTAA 2 cut(s) 68, 189
TscAI CASTG 1 cut(s) 262
TseFI GTSAC 2 cut(s) 586, 707
TseI GCWGC 5 cut(s) 19, 832, 951, 954, 1005
Tsp45I GTSAC 2 cut(s) 586, 707
TspDTI ATGAA 3 cut(s) 558, 663, 702
TspGWI ACGGA 2 cut(s) 748, 899
TspRI CASTG 1 cut(s) 262
VpaK11BI GGWCC 1 cut(s) 46
XagI CCTNNNNNAGG 1 cut(s) 452
XspI CTAG 1 cut(s) 879
ZrmI AGTACT 1 cut(s) 552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.