Rroxscaffold_3G00253320

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001740
Physical Location & Seq
Forward (+)
47363618 .. 47367640
4023 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00253320.1

Sequence Viewer

Length: 774 bp
ATGGCTGAGGGTCTGAAGCTGCCTATCATAGACTTGTCCTCACCGGACCGCACCTCCTCCGCCAGTTTAATCCGTCAGGCTTGCATAGAGTGTGGTTTCTTTTACTTGGTGAATCATGGTGTTGAGGAAGAGTTACTTGGCCAAGTGTTTGATCAGAGCAGTAAGTTTTTTTCACTTCCTTTGGAGGTCAAGTCCAAATTGGCGCGCAAACAAAACAGAGGCTACACTGCGCTCTTTGCTGAGAACCTTGACCCCAGTGCCAGCTCCAAAGGTGATTCAAAAGAGAGTTTTTACATTGGTCCTTTTGAAGATGAGACTCAAAATAAGTTGAATCAATGGCCTCCAGAAGATATCCTTCCATCCTGGCGACCTACAATGGAATCCTACTATAAAAAAGTTCTTTCTGCTGGGAAAAGAGTACTATCCCTGATTGCCCTGGCTTTGAACCTGGATGAGGATTTTTTTGAGAAAGTGGGGGATCTAAATAAACACACAGCAGTTCTTCGCCTTTTGCACTATCCAGGTCAGCTGGGATCATCTGATGAAGAAGTACTTGGTGCTTCTGCTCATTCAGATTATGGGATGATCACACTCCTGGCATCCAATGGTGTCCCAGGACTTCAGGTGGTTTTCTTCTTAGAACCCAATGAAGAAACTCTGGTAGAATGCCTGGCAAGTTGTTGCAATGAATCATTGCCGCCAAGATTTCCTCCCATCCGCAGCGGGGACTACCTGAAAGAGCGGTTTAGACTTACTTATGGCTCATCTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

257

Amino Acids

28.63

Weight (kDa)

4.95

Isoelectric Point (pI)

55.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 7 - 116 1.5e-31 non-haem dioxygenase in morphine synthesis N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 742
AccII CGCG 1 cut(s) 205
AciI CCGC 6 cut(s) 49, 60, 698, 718, 723, 742
AclWI GGATC 2 cut(s) 486, 541
AcoI YGGCCR 1 cut(s) 139
AcuI CTGAAG 2 cut(s) 35, 605
AfaI GTAC 2 cut(s) 420, 552
AfiI CCNNNNNNNGG 2 cut(s) 454, 724
AgsI TTSAA 4 cut(s) 279, 308, 331, 445
AjnI CCWGG 7 cut(s) 362, 435, 447, 520, 594, 613, 669
AjuI GAANNNNNNNTTGG 4 cut(s) 120, 152, 537, 569
AluBI AGCT 3 cut(s) 19, 264, 529
AluI AGCT 3 cut(s) 19, 264, 529
Alw26I GTCTC 1 cut(s) 308
AlwI GGATC 2 cut(s) 486, 541
AoxI GGCC 2 cut(s) 139, 338
ApeKI GCWGC 2 cut(s) 19, 720
AspLEI GCGC 3 cut(s) 205, 207, 232
AspS9I GGNCC 2 cut(s) 46, 299
AsuHPI GGTGA 3 cut(s) 33, 121, 284
AvaII GGWCC 2 cut(s) 46, 299
BalI TGGCCA 1 cut(s) 141
BbvCI CCTCAGC 1 cut(s) 6
BbvI GCAGC 2 cut(s) 6, 732
BccI CCATC 2 cut(s) 367, 722
BciT130I CCWGG 7 cut(s) 364, 437, 449, 522, 596, 615, 671
BclI TGATCA 2 cut(s) 151, 585
BcoDI GTCTC 1 cut(s) 308
BisI GCNGC 3 cut(s) 20, 698, 721
BlsI GCNGC 3 cut(s) 21, 699, 722
BmcAI AGTACT 2 cut(s) 420, 552
Bme1390I CCNGG 7 cut(s) 364, 437, 449, 522, 596, 615, 671
Bme18I GGWCC 2 cut(s) 46, 299
BmgT120I GGNCC 2 cut(s) 46, 299
BmrFI CCNGG 7 cut(s) 364, 437, 449, 522, 596, 615, 671
BmrI ACTGGG 1 cut(s) 249
BmsI GCATC 1 cut(s) 608
BmuI ACTGGG 1 cut(s) 249
BpmI CTGGAG 1 cut(s) 327
Bpu10I CCTNAGC 1 cut(s) 6
BsaJI CCNNGG 2 cut(s) 435, 613
BsaWI WCCGGW 1 cut(s) 43
BsaXI ACNNNNNCTCC 4 cut(s) 38, 68, 176, 206
Bsc4I CCNNNNNNNGG 2 cut(s) 454, 724
Bse1I ACTGG 2 cut(s) 63, 255
Bse3DI GCAATG 2 cut(s) 691, 692
BseBI CCWGG 7 cut(s) 364, 437, 449, 522, 596, 615, 671
BseDI CCNNGG 2 cut(s) 435, 613
BseGI GGATG 5 cut(s) 359, 457, 588, 599, 714
BseLI CCNNNNNNNGG 2 cut(s) 454, 724
BseMI GCAATG 2 cut(s) 691, 692
BseMII CTCAG 1 cut(s) 231
BseNI ACTGG 2 cut(s) 63, 255
BsePI GCGCGC 1 cut(s) 203
BseRI GAGGAG 1 cut(s) 46
BseXI GCAGC 2 cut(s) 6, 732
BseYI CCCAGC 2 cut(s) 407, 529
Bsh1236I CGCG 1 cut(s) 205
BshFI GGCC 2 cut(s) 141, 340
BsiSI CCGG 1 cut(s) 44
BslFI GGGAC 2 cut(s) 596, 740
BslI CCNNNNNNNGG 2 cut(s) 454, 724
BsmAI GTCTC 1 cut(s) 308
BsmFI GGGAC 2 cut(s) 596, 740
BsmI GAATGC 1 cut(s) 671
BsnI GGCC 2 cut(s) 141, 340
Bsp143I GATC 4 cut(s) 151, 478, 533, 585
BspACI CCGC 6 cut(s) 49, 60, 698, 718, 723, 742
BspANI GGCC 2 cut(s) 141, 340
BspCNI CTCAG 1 cut(s) 232
BspFNI CGCG 1 cut(s) 205
BspPI GGATC 2 cut(s) 486, 541
BsrBI CCGCTC 1 cut(s) 742
BsrDI GCAATG 2 cut(s) 691, 692
BsrI ACTGG 2 cut(s) 63, 255
BssECI CCNNGG 2 cut(s) 435, 613
BssHII GCGCGC 1 cut(s) 203
BssMI GATC 4 cut(s) 151, 478, 533, 585
Bst2UI CCWGG 7 cut(s) 364, 437, 449, 522, 596, 615, 671
Bst6I CTCTTC 1 cut(s) 123
BstC8I GCNNGC 3 cut(s) 82, 205, 262
BstDEI CTNAG 3 cut(s) 6, 240, 637
BstENI CCTNNNNNAGG 1 cut(s) 452
BstF5I GGATG 5 cut(s) 359, 457, 588, 599, 714
BstFNI CGCG 1 cut(s) 205
BstHHI GCGC 3 cut(s) 205, 207, 232
BstKTI GATC 4 cut(s) 154, 481, 536, 588
BstMAI GTCTC 1 cut(s) 308
BstMBI GATC 4 cut(s) 151, 478, 533, 585
BstMWI GCNNNNNNNGC 1 cut(s) 236
BstNI CCWGG 7 cut(s) 364, 437, 449, 522, 596, 615, 671
BstSCI CCNGG 7 cut(s) 362, 435, 447, 520, 594, 613, 669
BstUI CGCG 1 cut(s) 205
BstV1I GCAGC 2 cut(s) 6, 732
BstX2I RGATCY 1 cut(s) 478
BstYI RGATCY 1 cut(s) 478
BsuRI GGCC 2 cut(s) 141, 340
BtsCI GGATG 5 cut(s) 359, 457, 588, 599, 714
BtsI GCAGTG 1 cut(s) 225
BtsIMutI CAGTG 2 cut(s) 225, 262
Cac8I GCNNGC 3 cut(s) 82, 205, 262
CfoI GCGC 3 cut(s) 205, 207, 232
Cfr13I GGNCC 2 cut(s) 46, 299
CpoI CGGWCCG 1 cut(s) 46
Csp6I GTAC 2 cut(s) 419, 551
CspI CGGWCCG 1 cut(s) 46
CviAII CATG 1 cut(s) 116
CviQI GTAC 2 cut(s) 419, 551
DdeI CTNAG 3 cut(s) 6, 240, 637
DpnI GATC 4 cut(s) 153, 480, 535, 587
DpnII GATC 4 cut(s) 151, 478, 533, 585
EaeI YGGCCR 1 cut(s) 139
Eam1104I CTCTTC 1 cut(s) 123
EarI CTCTTC 1 cut(s) 123
EciI GGCGGA 1 cut(s) 49
Eco32I GATATC 1 cut(s) 352
Eco47I GGWCC 2 cut(s) 46, 299
Eco57I CTGAAG 2 cut(s) 35, 605
EcoNI CCTNNNNNAGG 1 cut(s) 452
EcoRII CCWGG 7 cut(s) 362, 435, 447, 520, 594, 613, 669
EcoRV GATATC 1 cut(s) 352
FaeI CATG 1 cut(s) 119
FaiI YATR 6 cut(s) 29, 86, 117, 390, 579, 759
FalI AAGNNNNNCTT 6 cut(s) 120, 152, 339, 371, 537, 569
FaqI GGGAC 2 cut(s) 596, 740
FatI CATG 1 cut(s) 115
FauI CCCGC 1 cut(s) 716
FbaI TGATCA 2 cut(s) 151, 585
Fnu4HI GCNGC 3 cut(s) 20, 698, 721
FokI GGATG 5 cut(s) 346, 464, 586, 595, 701
Fsp4HI GCNGC 3 cut(s) 20, 698, 721
GlaI GCGC 3 cut(s) 204, 206, 231
GluI GCNGC 3 cut(s) 20, 698, 721
GsaI CCCAGC 2 cut(s) 411, 533
GsuI CTGGAG 1 cut(s) 327
HaeIII GGCC 2 cut(s) 141, 340
HapII CCGG 1 cut(s) 44
HhaI GCGC 3 cut(s) 205, 207, 232
Hin1II CATG 1 cut(s) 119
Hin6I GCGC 3 cut(s) 203, 205, 230
HinP1I GCGC 3 cut(s) 203, 205, 230
HinfI GANTC 6 cut(s) 112, 275, 316, 331, 380, 689
HpaII CCGG 1 cut(s) 44
HphI GGTGA 3 cut(s) 33, 121, 284
Hpy188I TCNGA 5 cut(s) 15, 156, 541, 574, 769
Hpy188III TCNNGA 1 cut(s) 344
HpyAV CCTTC 1 cut(s) 365
HpyCH4V TGCA 3 cut(s) 84, 514, 684
HpyF10VI GCNNNNNNNGC 1 cut(s) 236
HpyF3I CTNAG 3 cut(s) 6, 240, 637
Hsp92II CATG 1 cut(s) 119
HspAI GCGC 3 cut(s) 203, 205, 230
Ksp22I TGATCA 2 cut(s) 151, 585
Kzo9I GATC 4 cut(s) 151, 478, 533, 585
LmnI GCTCC 1 cut(s) 269
Lsp1109I GCAGC 2 cut(s) 6, 732
LweI GCATC 1 cut(s) 608
MaeIII GTNAC 1 cut(s) 132
MalI GATC 4 cut(s) 153, 480, 535, 587
MbiI CCGCTC 1 cut(s) 742
MboI GATC 4 cut(s) 151, 478, 533, 585
MboII GAAGA 7 cut(s) 140, 320, 359, 494, 557, 625, 662
MflI RGATCY 1 cut(s) 478
MlsI TGGCCA 1 cut(s) 141
MluCI AATT 1 cut(s) 197
MluNI TGGCCA 1 cut(s) 141
MlyI GAGTC 1 cut(s) 310
MnlI CCTC 9 cut(s) 49, 64, 67, 118, 178, 212, 351, 448, 720
Mox20I TGGCCA 1 cut(s) 141
MscI TGGCCA 1 cut(s) 141
MseI TTAA 1 cut(s) 68
MslI CAYNNNNRTG 1 cut(s) 769
Msp20I TGGCCA 1 cut(s) 141
MspA1I CMGCKG 2 cut(s) 529, 723
MspI CCGG 1 cut(s) 44
MspR9I CCNGG 7 cut(s) 364, 437, 449, 522, 596, 615, 671
Mva1269I GAATGC 1 cut(s) 671
MvaI CCWGG 7 cut(s) 364, 437, 449, 522, 596, 615, 671
MvnI CGCG 1 cut(s) 205
MwoI GCNNNNNNNGC 1 cut(s) 236
NdeII GATC 4 cut(s) 151, 478, 533, 585
NlaIII CATG 1 cut(s) 119
PauI GCGCGC 1 cut(s) 203
PctI GAATGC 1 cut(s) 671
PfeI GAWTC 5 cut(s) 112, 275, 331, 380, 689
PkrI GCNGC 3 cut(s) 21, 699, 722
PleI GAGTC 1 cut(s) 310
PpsI GAGTC 1 cut(s) 310
Psp6I CCWGG 7 cut(s) 362, 435, 447, 520, 594, 613, 669
PspFI CCCAGC 2 cut(s) 407, 529
PspGI CCWGG 7 cut(s) 362, 435, 447, 520, 594, 613, 669
PspPI GGNCC 2 cut(s) 46, 299
PsuI RGATCY 1 cut(s) 478
PteI GCGCGC 1 cut(s) 203
PvuII CAGCTG 1 cut(s) 529
RsaI GTAC 2 cut(s) 420, 552
RsaNI GTAC 2 cut(s) 419, 551
RseI CAYNNNNRTG 1 cut(s) 769
Rsr2I CGGWCCG 1 cut(s) 46
RsrII CGGWCCG 1 cut(s) 46
SaqAI TTAA 1 cut(s) 68
SatI GCNGC 3 cut(s) 20, 698, 721
Sau3AI GATC 4 cut(s) 151, 478, 533, 585
Sau96I GGNCC 2 cut(s) 46, 299
ScaI AGTACT 2 cut(s) 420, 552
SchI GAGTC 1 cut(s) 310
ScrFI CCNGG 7 cut(s) 364, 437, 449, 522, 596, 615, 671
SfaNI GCATC 1 cut(s) 608
SinI GGWCC 2 cut(s) 46, 299
SmiMI CAYNNNNRTG 1 cut(s) 769
Sse9I AATT 1 cut(s) 197
SsiI CCGC 6 cut(s) 49, 60, 698, 718, 723, 742
StyD4I CCNGG 7 cut(s) 362, 435, 447, 520, 594, 613, 669
TasI AATT 1 cut(s) 197
TatI WGTACW 2 cut(s) 418, 550
TauI GCSGC 1 cut(s) 700
TfiI GAWTC 5 cut(s) 112, 275, 331, 380, 689
Tru1I TTAA 1 cut(s) 68
Tru9I TTAA 1 cut(s) 68
TscAI CASTG 2 cut(s) 232, 262
TseI GCWGC 2 cut(s) 19, 720
TspDTI ATGAA 3 cut(s) 558, 663, 702
TspGWI ACGGA 1 cut(s) 62
TspRI CASTG 2 cut(s) 232, 262
VpaK11BI GGWCC 2 cut(s) 46, 299
XagI CCTNNNNNAGG 1 cut(s) 452
ZrmI AGTACT 2 cut(s) 420, 552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.