AT4G16770

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0001740
Physical Location & Seq
Reverse (-)
9433955 .. 9437206
3252 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G16770.1

Sequence Viewer

Length: 777 bp
ATGAGAGAAATCCAAATGAAATATAATAGGGATAAAAAGAACAAAATGGGAACAGCTCTGAAACTTCCGATCATCGATCTTTCTTCGCCGGAGAAGCTCTCTACTTCCCGATTGATTCGTCAGGCTTGCTTGGATCATGGATTCTTCTATCTTACGAACCATGGCGTTTCGGAAGAGTTGATGGAAGGAGTGTTAATAGAGAGCAAGAAACTATTCTCCCTTCCTCTTGATGAGAAGATGGTGATGGCTCGTCATGGTTTTCGTGGTTACTCACCGTTATACGACGAGAAACTTGAATCATCTTCCACCTCCATAGGTGATTCCAAGGAGATGTTCACTTTTGGATCTTCAGAAGGAGTTTTGGGTCAACTTTACCCTAACAAGTGGCCTCTTGAAGAGCTTTTGCCGCTTTGGAGGCCAACCATGGAATGCTACTATAAAAATGTCATGGATGTTGGTAAGAAATTGTTTGGCCTAGTGGCTTTGGCATTGAATTTAGAGGAAAACTACTTTGAACAAGTGGGAGCTTTCAATGATCAAGCAGCGGTTGTTCGTCTCTTACGTTATTCAGGAGAATCAAATTCATCTGGAGAAGAAACATGTGGTGCCTCTGCTCATTCAGATTTTGGAATGATAACTCTTCTTGCAACTGATGGAGTTGCAGGGCTTCAAGTTTGTAGAGATAAAGATAAAGAACCAAAAGTTTGGGAAGATGTCGCTGGTATTAAAGGGGACTTTTGTTGTCAACATCGGCGACCTTATGGAAAGATGGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

258

Amino Acids

29.08

Weight (kDa)

5.67

Isoelectric Point (pI)

44.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 22 - 130 3.1e-27 non-haem dioxygenase in morphine synthesis N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 605
AciI CCGC 2 cut(s) 407, 545
AclWI GGATC 2 cut(s) 141, 352
AcsI RAATTY 2 cut(s) 493, 580
AcuI CTGAAG 1 cut(s) 333
AflIII ACRYGT 1 cut(s) 599
AgsI TTSAA 6 cut(s) 296, 395, 493, 515, 532, 671
AjuI GAANNNNNNNTTGG 2 cut(s) 317, 349
AluBI AGCT 4 cut(s) 56, 97, 400, 527
AluI AGCT 4 cut(s) 56, 97, 400, 527
Alw26I GTCTC 1 cut(s) 560
AlwI GGATC 2 cut(s) 141, 352
AoxI GGCC 3 cut(s) 386, 416, 472
ApeKI GCWGC 1 cut(s) 542
ApoI RAATTY 2 cut(s) 493, 580
Asp700I GAANNNNTTC 1 cut(s) 212
AsuHPI GGTGA 3 cut(s) 253, 264, 329
BanI GGYRCC 1 cut(s) 605
BbvI GCAGC 1 cut(s) 554
BccI CCATC 5 cut(s) 175, 232, 238, 647, 763
BclI TGATCA 1 cut(s) 535
BcoDI GTCTC 1 cut(s) 560
BfaI CTAG 1 cut(s) 476
BisI GCNGC 2 cut(s) 407, 543
BlsI GCNGC 2 cut(s) 408, 544
BmiI GGNNCC 1 cut(s) 607
BplI GAGNNNNNCTC 2 cut(s) 83, 115
BpmI CTGGAG 1 cut(s) 609
Bsa29I ATCGAT 1 cut(s) 75
BsaJI CCNNGG 3 cut(s) 160, 324, 423
BseCI ATCGAT 1 cut(s) 75
BseDI CCNNGG 3 cut(s) 160, 324, 423
BseGI GGATG 1 cut(s) 457
BseXI GCAGC 1 cut(s) 554
BshFI GGCC 3 cut(s) 388, 418, 474
BshNI GGYRCC 1 cut(s) 605
BshVI ATCGAT 1 cut(s) 75
BsiSI CCGG 1 cut(s) 89
BslFI GGGAC 1 cut(s) 746
BsmAI GTCTC 1 cut(s) 560
BsmBI CGTCTC 1 cut(s) 560
BsmFI GGGAC 1 cut(s) 746
BsmI GAATGC 1 cut(s) 434
BsnI GGCC 3 cut(s) 388, 418, 474
Bsp143I GATC 5 cut(s) 69, 76, 133, 344, 535
Bsp19I CCATGG 2 cut(s) 160, 423
BspACI CCGC 2 cut(s) 407, 545
BspANI GGCC 3 cut(s) 388, 418, 474
BspDI ATCGAT 1 cut(s) 75
BspLI GGNNCC 1 cut(s) 607
BspPI GGATC 2 cut(s) 141, 352
BspQI GCTCTTC 1 cut(s) 390
BspT107I GGYRCC 1 cut(s) 605
BssECI CCNNGG 3 cut(s) 160, 324, 423
BssMI GATC 5 cut(s) 69, 76, 133, 344, 535
BssT1I CCWWGG 3 cut(s) 160, 324, 423
Bst4CI ACNGT 1 cut(s) 276
Bst6I CTCTTC 3 cut(s) 168, 390, 645
BstC8I GCNNGC 1 cut(s) 127
BstDSI CCRYGG 2 cut(s) 160, 423
BstF5I GGATG 1 cut(s) 457
BstKTI GATC 5 cut(s) 72, 79, 136, 347, 538
BstMAI GTCTC 1 cut(s) 560
BstMBI GATC 5 cut(s) 69, 76, 133, 344, 535
BstMWI GCNNNNNNNGC 3 cut(s) 94, 406, 415
BstNSI RCATGY 1 cut(s) 603
BstV1I GCAGC 1 cut(s) 554
BstX2I RGATCY 1 cut(s) 344
BstXI CCANNNNNNTGG 1 cut(s) 705
BstYI RGATCY 1 cut(s) 344
Bsu15I ATCGAT 1 cut(s) 75
BsuRI GGCC 3 cut(s) 388, 418, 474
BsuTUI ATCGAT 1 cut(s) 75
BtgI CCRYGG 2 cut(s) 160, 423
BtsCI GGATG 1 cut(s) 457
Cac8I GCNNGC 1 cut(s) 127
ClaI ATCGAT 1 cut(s) 75
CviAII CATG 6 cut(s) 137, 161, 254, 424, 448, 600
DpnI GATC 5 cut(s) 71, 78, 135, 346, 537
DpnII GATC 5 cut(s) 69, 76, 133, 344, 535
Eam1104I CTCTTC 3 cut(s) 168, 390, 645
EarI CTCTTC 3 cut(s) 168, 390, 645
Eco130I CCWWGG 3 cut(s) 160, 324, 423
Eco57I CTGAAG 1 cut(s) 333
EcoT14I CCWWGG 3 cut(s) 160, 324, 423
ErhI CCWWGG 3 cut(s) 160, 324, 423
Esp3I CGTCTC 1 cut(s) 560
FaeI CATG 6 cut(s) 140, 164, 257, 427, 451, 603
FaqI GGGAC 1 cut(s) 746
FatI CATG 6 cut(s) 136, 160, 253, 423, 447, 599
FbaI TGATCA 1 cut(s) 535
Fnu4HI GCNGC 2 cut(s) 407, 543
FokI GGATG 1 cut(s) 464
Fsp4HI GCNGC 2 cut(s) 407, 543
FspBI CTAG 1 cut(s) 476
GluI GCNGC 2 cut(s) 407, 543
GsuI CTGGAG 1 cut(s) 609
HaeIII GGCC 3 cut(s) 388, 418, 474
HapII CCGG 1 cut(s) 89
Hin1II CATG 6 cut(s) 140, 164, 257, 427, 451, 603
HincII GTYRAC 2 cut(s) 368, 746
HindII GTYRAC 2 cut(s) 368, 746
HinfI GANTC 5 cut(s) 115, 141, 296, 320, 575
HpaII CCGG 1 cut(s) 89
HphI GGTGA 3 cut(s) 253, 264, 329
Hpy166II GTNNAC 3 cut(s) 336, 368, 746
Hpy188I TCNGA 5 cut(s) 60, 69, 172, 352, 622
Hpy188III TCNNGA 5 cut(s) 108, 227, 392, 570, 588
Hpy8I GTNNAC 3 cut(s) 336, 368, 746
Hpy99I CGWCG 1 cut(s) 287
HpyAV CCTTC 3 cut(s) 179, 230, 347
HpyCH4III ACNGT 1 cut(s) 276
HpyCH4IV ACGT 1 cut(s) 562
HpyCH4V TGCA 2 cut(s) 647, 662
HpyF10VI GCNNNNNNNGC 3 cut(s) 94, 406, 415
HpySE526I ACGT 1 cut(s) 562
Hsp92II CATG 6 cut(s) 140, 164, 257, 427, 451, 603
Ksp22I TGATCA 1 cut(s) 535
Kzo9I GATC 5 cut(s) 69, 76, 133, 344, 535
LguI GCTCTTC 1 cut(s) 390
LmnI GCTCC 1 cut(s) 524
LpnPI CCDG 6 cut(s) 102, 107, 555, 573, 648, 705
Lsp1109I GCAGC 1 cut(s) 554
MaeI CTAG 1 cut(s) 476
MaeII ACGT 1 cut(s) 562
MaeIII GTNAC 1 cut(s) 266
MalI GATC 5 cut(s) 71, 78, 135, 346, 537
MboI GATC 5 cut(s) 69, 76, 133, 344, 535
MflI RGATCY 1 cut(s) 344
MluCI AATT 3 cut(s) 464, 493, 580
MnlI CCTC 6 cut(s) 234, 319, 399, 408, 493, 619
MroXI GAANNNNTTC 1 cut(s) 212
MseI TTAA 2 cut(s) 194, 726
MspA1I CMGCKG 1 cut(s) 545
MspI CCGG 1 cut(s) 89
Mva1269I GAATGC 1 cut(s) 434
MwoI GCNNNNNNNGC 3 cut(s) 94, 406, 415
NcoI CCATGG 2 cut(s) 160, 423
NdeII GATC 5 cut(s) 69, 76, 133, 344, 535
NlaIII CATG 6 cut(s) 140, 164, 257, 427, 451, 603
NlaIV GGNNCC 1 cut(s) 607
NspI RCATGY 1 cut(s) 603
PciI ACATGT 1 cut(s) 599
PciSI GCTCTTC 1 cut(s) 390
PcsI WCGNNNNNNNCGW 1 cut(s) 559
PctI GAATGC 1 cut(s) 434
PdmI GAANNNNTTC 1 cut(s) 212
PfeI GAWTC 5 cut(s) 115, 141, 296, 320, 575
PkrI GCNGC 2 cut(s) 408, 544
PscI ACATGT 1 cut(s) 599
PspN4I GGNNCC 1 cut(s) 607
PsuI RGATCY 1 cut(s) 344
SapI GCTCTTC 1 cut(s) 390
SaqAI TTAA 2 cut(s) 194, 726
SatI GCNGC 2 cut(s) 407, 543
Sau3AI GATC 5 cut(s) 69, 76, 133, 344, 535
SetI ASST 8 cut(s) 58, 99, 311, 319, 402, 529, 565, 760
Sse9I AATT 3 cut(s) 464, 493, 580
SsiI CCGC 2 cut(s) 407, 545
SspMI CTAG 1 cut(s) 476
StyI CCWWGG 3 cut(s) 160, 324, 423
TaaI ACNGT 1 cut(s) 276
TaiI ACGT 1 cut(s) 565
TaqI TCGA 1 cut(s) 75
TasI AATT 3 cut(s) 464, 493, 580
TauI GCSGC 1 cut(s) 409
TfiI GAWTC 5 cut(s) 115, 141, 296, 320, 575
Tru1I TTAA 2 cut(s) 194, 726
Tru9I TTAA 2 cut(s) 194, 726
TseI GCWGC 1 cut(s) 542
TspDTI ATGAA 2 cut(s) 32, 573
XapI RAATTY 2 cut(s) 493, 580
XceI RCATGY 1 cut(s) 603
XmnI GAANNNNTTC 1 cut(s) 212
XspI CTAG 1 cut(s) 476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.