Rh7DG217500

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0001740
Physical Location & Seq
Reverse (-)
21051780 .. 21054714
2935 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG217500.1

Sequence Viewer

Length: 708 bp
ATGGCGGAGGGTCTGAAGCTACCTATCATAGACTTGTCCTCACCGGACCGCACCTCCTCCGCCAGATTAATCCGTCAGGCTTGCATGGAGTGTGGTTTCTTTTACTTGGTGAATCATGGTGTTGAGGAAGAGTTACTTGGCCGAGTGTTTGATCAGAGCAGTAAGTTTTTTTCACTTCCTTTGGAAGTTAAGTCCAAATTGGCGCGCAAACAAAACAGAGGCTACACAGCGCTCTTTGCTGAGAACCTTGACCCCACTGCCAGCTCCAAAGGTGATTCAAAAGAGAGTTTTTACATTGGCCCTTTAGAAGACGCAACTCAAAATAAGTTGAATCAATGGCCTTCAGAAGATATCCTTCCATCCTGGAGACCTACAATGGAATCCTACTATAAAAAAGTTCTTTCAACTGGGAAAAGATTATTATCTCTGATTGCCCTGGCTTTGAACCTGGATGAGGATTTTTTTGAGAAAGTGGGGGGTCTGAATAAACACACAGCACTTCTTCGCCTTTTGCACTACCCAGGTCAACTGGGATCATCTGATGAAGAAGTACTTGGTGCTTCTGCTCATTCAGATTATGGAACAGTCACACTCCTGGCATCCAATGGTGTCCCAGGACTTCAGGTGGCTTTCTTCTTAGACCCCAATGAAGACTCTCTGGTAGAATGCCTGGCAAGTTGTTGCAATGAATCATTGCCGCCAAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

235

Amino Acids

25.94

Weight (kDa)

5.09

Isoelectric Point (pI)

51.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 7 - 116 8.5e-32 non-haem dioxygenase in morphine synthesis N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 205
AciI CCGC 4 cut(s) 5, 49, 60, 698
AclWI GGATC 1 cut(s) 541
AcoI YGGCCR 1 cut(s) 139
AcuI CTGAAG 3 cut(s) 35, 327, 605
AfaI GTAC 1 cut(s) 552
AfeI AGCGCT 1 cut(s) 231
AfiI CCNNNNNNNGG 1 cut(s) 454
AgsI TTSAA 4 cut(s) 279, 331, 405, 445
AjnI CCWGG 7 cut(s) 362, 435, 447, 520, 594, 613, 669
AjuI GAANNNNNNNTTGG 4 cut(s) 120, 152, 537, 569
AluBI AGCT 2 cut(s) 19, 264
AluI AGCT 2 cut(s) 19, 264
Alw26I GTCTC 1 cut(s) 361
AlwI GGATC 1 cut(s) 541
Aor51HI AGCGCT 1 cut(s) 231
AoxI GGCC 3 cut(s) 139, 298, 338
AseI ATTAAT 1 cut(s) 68
AspLEI GCGC 3 cut(s) 205, 207, 232
AspS9I GGNCC 2 cut(s) 46, 299
AsuHPI GGTGA 3 cut(s) 33, 121, 284
AvaII GGWCC 1 cut(s) 46
BbsI GAAGAC 2 cut(s) 315, 657
BccI CCATC 1 cut(s) 367
BciT130I CCWGG 7 cut(s) 364, 437, 449, 522, 596, 615, 671
BclI TGATCA 1 cut(s) 151
BcoDI GTCTC 1 cut(s) 361
BfoI RGCGCY 1 cut(s) 233
BisI GCNGC 1 cut(s) 698
BlsI GCNGC 1 cut(s) 699
BmcAI AGTACT 1 cut(s) 552
Bme1390I CCNGG 7 cut(s) 364, 437, 449, 522, 596, 615, 671
Bme18I GGWCC 1 cut(s) 46
BmgT120I GGNCC 2 cut(s) 46, 299
BmrFI CCNGG 7 cut(s) 364, 437, 449, 522, 596, 615, 671
BmrI ACTGGG 2 cut(s) 417, 539
BmsI GCATC 1 cut(s) 608
BmuI ACTGGG 2 cut(s) 417, 539
BpiI GAAGAC 2 cut(s) 315, 657
BpmI CTGGAG 1 cut(s) 385
BsaBI GATNNNNATC 1 cut(s) 421
BsaI GGTCTC 1 cut(s) 361
BsaJI CCNNGG 4 cut(s) 435, 520, 613, 701
BsaWI WCCGGW 1 cut(s) 43
BsaXI ACNNNNNCTCC 2 cut(s) 38, 68
Bsc4I CCNNNNNNNGG 1 cut(s) 454
Bse1I ACTGG 2 cut(s) 412, 534
Bse3DI GCAATG 2 cut(s) 691, 692
Bse8I GATNNNNATC 1 cut(s) 421
BseBI CCWGG 7 cut(s) 364, 437, 449, 522, 596, 615, 671
BseDI CCNNGG 4 cut(s) 435, 520, 613, 701
BseGI GGATG 3 cut(s) 359, 457, 599
BseJI GATNNNNATC 1 cut(s) 421
BseLI CCNNNNNNNGG 1 cut(s) 454
BseMI GCAATG 2 cut(s) 691, 692
BseMII CTCAG 1 cut(s) 231
BseNI ACTGG 2 cut(s) 412, 534
BsePI GCGCGC 1 cut(s) 203
BseRI GAGGAG 1 cut(s) 46
Bsh1236I CGCG 1 cut(s) 205
BshFI GGCC 3 cut(s) 141, 300, 340
BsiSI CCGG 1 cut(s) 44
BslFI GGGAC 1 cut(s) 596
BslI CCNNNNNNNGG 1 cut(s) 454
BsmAI GTCTC 1 cut(s) 361
BsmFI GGGAC 1 cut(s) 596
BsmI GAATGC 1 cut(s) 671
BsnI GGCC 3 cut(s) 141, 300, 340
Bso31I GGTCTC 1 cut(s) 361
Bsp143I GATC 2 cut(s) 151, 533
BspACI CCGC 4 cut(s) 5, 49, 60, 698
BspANI GGCC 3 cut(s) 141, 300, 340
BspCNI CTCAG 1 cut(s) 232
BspFNI CGCG 1 cut(s) 205
BspPI GGATC 1 cut(s) 541
BspTNI GGTCTC 1 cut(s) 361
BsrDI GCAATG 2 cut(s) 691, 692
BsrI ACTGG 2 cut(s) 412, 534
BssECI CCNNGG 4 cut(s) 435, 520, 613, 701
BssHII GCGCGC 1 cut(s) 203
BssMI GATC 2 cut(s) 151, 533
BssT1I CCWWGG 1 cut(s) 701
Bst2UI CCWGG 7 cut(s) 364, 437, 449, 522, 596, 615, 671
Bst4CI ACNGT 1 cut(s) 586
Bst6I CTCTTC 1 cut(s) 123
BstC8I GCNNGC 3 cut(s) 82, 205, 262
BstDEI CTNAG 2 cut(s) 240, 637
BstENI CCTNNNNNAGG 1 cut(s) 452
BstF5I GGATG 3 cut(s) 359, 457, 599
BstFNI CGCG 1 cut(s) 205
BstH2I RGCGCY 1 cut(s) 233
BstHHI GCGC 3 cut(s) 205, 207, 232
BstKTI GATC 2 cut(s) 154, 536
BstMAI GTCTC 1 cut(s) 361
BstMBI GATC 2 cut(s) 151, 533
BstMWI GCNNNNNNNGC 1 cut(s) 236
BstNI CCWGG 7 cut(s) 364, 437, 449, 522, 596, 615, 671
BstSCI CCNGG 7 cut(s) 362, 435, 447, 520, 594, 613, 669
BstUI CGCG 1 cut(s) 205
BstV2I GAAGAC 2 cut(s) 315, 657
BsuRI GGCC 3 cut(s) 141, 300, 340
BtsCI GGATG 3 cut(s) 359, 457, 599
BtsI GCAGTG 1 cut(s) 255
BtsIMutI CAGTG 1 cut(s) 255
Cac8I GCNNGC 3 cut(s) 82, 205, 262
CfoI GCGC 3 cut(s) 205, 207, 232
Cfr13I GGNCC 2 cut(s) 46, 299
CpoI CGGWCCG 1 cut(s) 46
CseI GACGC 1 cut(s) 320
Csp6I GTAC 1 cut(s) 551
CspI CGGWCCG 1 cut(s) 46
CviAII CATG 2 cut(s) 85, 116
CviJI RGCY 9 cut(s) 19, 80, 141, 222, 264, 300, 340, 440, 629
CviKI_1 RGCY 9 cut(s) 19, 80, 141, 222, 264, 300, 340, 440, 629
CviQI GTAC 1 cut(s) 551
DdeI CTNAG 2 cut(s) 240, 637
DpnI GATC 2 cut(s) 153, 535
DpnII GATC 2 cut(s) 151, 533
EaeI YGGCCR 1 cut(s) 139
Eam1104I CTCTTC 1 cut(s) 123
EarI CTCTTC 1 cut(s) 123
EciI GGCGGA 2 cut(s) 20, 49
Eco130I CCWWGG 1 cut(s) 701
Eco31I GGTCTC 1 cut(s) 361
Eco32I GATATC 1 cut(s) 352
Eco47I GGWCC 1 cut(s) 46
Eco47III AGCGCT 1 cut(s) 231
Eco57I CTGAAG 3 cut(s) 35, 327, 605
EcoNI CCTNNNNNAGG 1 cut(s) 452
EcoRII CCWGG 7 cut(s) 362, 435, 447, 520, 594, 613, 669
EcoRV GATATC 1 cut(s) 352
EcoT14I CCWWGG 1 cut(s) 701
ErhI CCWWGG 1 cut(s) 701
FaeI CATG 2 cut(s) 88, 119
FaiI YATR 5 cut(s) 29, 86, 117, 390, 579
FalI AAGNNNNNCTT 6 cut(s) 120, 152, 339, 371, 537, 569
FaqI GGGAC 1 cut(s) 596
FatI CATG 2 cut(s) 84, 115
FbaI TGATCA 1 cut(s) 151
Fnu4HI GCNGC 1 cut(s) 698
FokI GGATG 3 cut(s) 346, 464, 586
Fsp4HI GCNGC 1 cut(s) 698
GlaI GCGC 3 cut(s) 204, 206, 231
GluI GCNGC 1 cut(s) 698
GsuI CTGGAG 1 cut(s) 385
HaeII RGCGCY 1 cut(s) 233
HaeIII GGCC 3 cut(s) 141, 300, 340
HapII CCGG 1 cut(s) 44
HgaI GACGC 1 cut(s) 320
HhaI GCGC 3 cut(s) 205, 207, 232
Hin1II CATG 2 cut(s) 88, 119
Hin6I GCGC 3 cut(s) 203, 205, 230
HinP1I GCGC 3 cut(s) 203, 205, 230
HincII GTYRAC 1 cut(s) 527
HindII GTYRAC 1 cut(s) 527
HinfI GANTC 6 cut(s) 112, 275, 331, 380, 653, 689
HpaII CCGG 1 cut(s) 44
HphI GGTGA 3 cut(s) 33, 121, 284
Hpy166II GTNNAC 1 cut(s) 527
Hpy188I TCNGA 7 cut(s) 15, 156, 346, 429, 483, 541, 574
Hpy8I GTNNAC 1 cut(s) 527
HpyAV CCTTC 2 cut(s) 351, 365
HpyCH4III ACNGT 1 cut(s) 586
HpyCH4V TGCA 3 cut(s) 84, 514, 684
HpyF10VI GCNNNNNNNGC 1 cut(s) 236
HpyF3I CTNAG 2 cut(s) 240, 637
Hsp92II CATG 2 cut(s) 88, 119
HspAI GCGC 3 cut(s) 203, 205, 230
Ksp22I TGATCA 1 cut(s) 151
Kzo9I GATC 2 cut(s) 151, 533
LmnI GCTCC 1 cut(s) 269
LweI GCATC 1 cut(s) 608
MaeIII GTNAC 2 cut(s) 132, 586
MalI GATC 2 cut(s) 153, 535
MboI GATC 2 cut(s) 151, 533
MboII GAAGA 7 cut(s) 140, 320, 359, 494, 557, 625, 662
MluCI AATT 1 cut(s) 197
MlyI GAGTC 1 cut(s) 647
MnlI CCTC 6 cut(s) 49, 64, 67, 118, 212, 448
MseI TTAA 2 cut(s) 68, 189
MspI CCGG 1 cut(s) 44
MspR9I CCNGG 7 cut(s) 364, 437, 449, 522, 596, 615, 671
Mva1269I GAATGC 1 cut(s) 671
MvaI CCWGG 7 cut(s) 364, 437, 449, 522, 596, 615, 671
MvnI CGCG 1 cut(s) 205
MwoI GCNNNNNNNGC 1 cut(s) 236
NdeII GATC 2 cut(s) 151, 533
NlaIII CATG 2 cut(s) 88, 119
NmeAIII GCCGAG 1 cut(s) 167
NmuCI GTSAC 1 cut(s) 586
PauI GCGCGC 1 cut(s) 203
PctI GAATGC 1 cut(s) 671
PfeI GAWTC 5 cut(s) 112, 275, 331, 380, 689
PfoI TCCNGGA 1 cut(s) 362
PkrI GCNGC 1 cut(s) 699
PleI GAGTC 1 cut(s) 647
PpsI GAGTC 1 cut(s) 647
PshBI ATTAAT 1 cut(s) 68
Psp6I CCWGG 7 cut(s) 362, 435, 447, 520, 594, 613, 669
PspGI CCWGG 7 cut(s) 362, 435, 447, 520, 594, 613, 669
PspPI GGNCC 2 cut(s) 46, 299
PteI GCGCGC 1 cut(s) 203
RsaI GTAC 1 cut(s) 552
RsaNI GTAC 1 cut(s) 551
Rsr2I CGGWCCG 1 cut(s) 46
RsrII CGGWCCG 1 cut(s) 46
SaqAI TTAA 2 cut(s) 68, 189
SatI GCNGC 1 cut(s) 698
Sau3AI GATC 2 cut(s) 151, 533
Sau96I GGNCC 2 cut(s) 46, 299
ScaI AGTACT 1 cut(s) 552
SchI GAGTC 1 cut(s) 647
ScrFI CCNGG 7 cut(s) 364, 437, 449, 522, 596, 615, 671
SfaNI GCATC 1 cut(s) 608
SinI GGWCC 1 cut(s) 46
Sse9I AATT 1 cut(s) 197
SsiI CCGC 4 cut(s) 5, 49, 60, 698
StyD4I CCNGG 7 cut(s) 362, 435, 447, 520, 594, 613, 669
StyI CCWWGG 1 cut(s) 701
TaaI ACNGT 1 cut(s) 586
TasI AATT 1 cut(s) 197
TatI WGTACW 1 cut(s) 550
TauI GCSGC 1 cut(s) 700
TfiI GAWTC 5 cut(s) 112, 275, 331, 380, 689
Tru1I TTAA 2 cut(s) 68, 189
Tru9I TTAA 2 cut(s) 68, 189
TscAI CASTG 1 cut(s) 262
TseFI GTSAC 1 cut(s) 586
Tsp45I GTSAC 1 cut(s) 586
TspDTI ATGAA 3 cut(s) 558, 663, 702
TspGWI ACGGA 1 cut(s) 62
TspRI CASTG 1 cut(s) 262
VpaK11BI GGWCC 1 cut(s) 46
VspI ATTAAT 1 cut(s) 68
XagI CCTNNNNNAGG 1 cut(s) 452
ZrmI AGTACT 1 cut(s) 552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.