AT4G25040

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0003826
Physical Location & Seq
Forward (+)
12868169 .. 12869495
1327 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G25040.1

Sequence Viewer

Length: 513 bp
ATGATGGGAGACAATGAAGGAAGAAGAACTCCGTTATTGAATCTTGGGGTTCAAGTATCGATGAGAGTGTTAACCATCGGAGCTGCAATGGCCTCGATGTGGGTCATGATCACTAACCGTGAAGTCGCATCTGTTTATGGCATCGCTTTTGAGGCTAAATACAGCTACTCCTCTGCCTTTAGGTATTTGGTGTACGCACAGATAGCTGTCTGCGCCGCGACATTGTTTACGCTTGTATGGGCTTGTTTGGCAGTCCGCAGAAGAGGACTTGTTTTTGCACTCTTCTTCTTTGATTTGCTTACGACTTTGACGGCGATATCAGCTTTCTCTGCTGCCTTCGCGGAAGGTTACGTAGGGAAGTACGGTAACAAACAGGCCGGTTGGCTTCCGATCTGCGGTTATGTGCATGGTTATTGCAGTCGTGTCACTATCTCCCTCGCTATGTCCTTCGCCTCTTTTATCCTCTTGTTCATCCTTACGGTCTTAACTGCCTCCGCTGCTCGTCATTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0048037 GO:0051536 GO:0051540
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

170

Amino Acids

18.67

Weight (kDa)

9.47

Isoelectric Point (pI)

31.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 17 - 155 4.3e-35 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 218, 341
AciI CCGC 5 cut(s) 216, 256, 341, 396, 495
AfaI GTAC 2 cut(s) 194, 362
AfiI CCNNNNNNNGG 2 cut(s) 99, 395
AgsI TTSAA 2 cut(s) 40, 53
AluBI AGCT 4 cut(s) 83, 165, 206, 323
AluI AGCT 4 cut(s) 83, 165, 206, 323
Alw26I GTCTC 1 cut(s) 3
AoxI GGCC 2 cut(s) 90, 375
ApeKI GCWGC 3 cut(s) 83, 332, 497
AspLEI GCGC 1 cut(s) 215
BbvI GCAGC 3 cut(s) 70, 319, 484
BccI CCATC 1 cut(s) 83
BceAI ACGGC 1 cut(s) 327
BcgI CGANNNNNNTGC 2 cut(s) 75, 109
BclI TGATCA 1 cut(s) 108
BcoDI GTCTC 1 cut(s) 3
BisI GCNGC 4 cut(s) 84, 216, 333, 498
BlsI GCNGC 4 cut(s) 85, 217, 334, 499
BmsI GCATC 2 cut(s) 137, 150
Bsa29I ATCGAT 1 cut(s) 59
BsaAI YACGTR 1 cut(s) 352
BsaXI ACNNNNNCTCC 2 cut(s) 152, 182
Bsc4I CCNNNNNNNGG 2 cut(s) 99, 395
Bse118I RCCGGY 1 cut(s) 377
Bse3DI GCAATG 1 cut(s) 93
BseCI ATCGAT 1 cut(s) 59
BseGI GGATG 1 cut(s) 471
BseLI CCNNNNNNNGG 2 cut(s) 99, 395
BseMI GCAATG 1 cut(s) 93
BseRI GAGGAG 1 cut(s) 160
BseXI GCAGC 3 cut(s) 70, 319, 484
Bsh1236I CGCG 2 cut(s) 218, 341
BshFI GGCC 2 cut(s) 92, 377
BshVI ATCGAT 1 cut(s) 59
BsiSI CCGG 1 cut(s) 378
BslI CCNNNNNNNGG 2 cut(s) 99, 395
BsmAI GTCTC 1 cut(s) 3
BsnI GGCC 2 cut(s) 92, 377
Bsp143I GATC 2 cut(s) 108, 390
BspACI CCGC 5 cut(s) 216, 256, 341, 396, 495
BspANI GGCC 2 cut(s) 92, 377
BspDI ATCGAT 1 cut(s) 59
BspFNI CGCG 2 cut(s) 218, 341
BspHI TCATGA 1 cut(s) 105
BsrDI GCAATG 1 cut(s) 93
BsrFI RCCGGY 1 cut(s) 377
BssAI RCCGGY 1 cut(s) 377
BssMI GATC 2 cut(s) 108, 390
Bst4CI ACNGT 3 cut(s) 119, 365, 481
Bst6I CTCTTC 2 cut(s) 256, 287
BstBAI YACGTR 1 cut(s) 352
BstF5I GGATG 1 cut(s) 471
BstFNI CGCG 2 cut(s) 218, 341
BstHHI GCGC 1 cut(s) 215
BstKTI GATC 2 cut(s) 111, 393
BstMAI GTCTC 1 cut(s) 3
BstMBI GATC 2 cut(s) 108, 390
BstMWI GCNNNNNNNGC 9 cut(s) 89, 152, 203, 212, 248, 320, 329, 338, 497
BstSNI TACGTA 1 cut(s) 352
BstUI CGCG 2 cut(s) 218, 341
BstV1I GCAGC 3 cut(s) 70, 319, 484
Bsu15I ATCGAT 1 cut(s) 59
BsuRI GGCC 2 cut(s) 92, 377
BsuTUI ATCGAT 1 cut(s) 59
BtgZI GCGATG 1 cut(s) 127
BtsCI GGATG 1 cut(s) 471
CciI TCATGA 1 cut(s) 105
CfoI GCGC 1 cut(s) 215
Cfr10I RCCGGY 1 cut(s) 377
ClaI ATCGAT 1 cut(s) 59
Csp6I GTAC 2 cut(s) 193, 361
CviAII CATG 2 cut(s) 106, 407
CviJI RGCY 9 cut(s) 83, 92, 155, 165, 206, 242, 323, 377, 385
CviKI_1 RGCY 9 cut(s) 83, 92, 155, 165, 206, 242, 323, 377, 385
CviQI GTAC 2 cut(s) 193, 361
DpnI GATC 2 cut(s) 110, 392
DpnII GATC 2 cut(s) 108, 390
Eam1104I CTCTTC 2 cut(s) 256, 287
EarI CTCTTC 2 cut(s) 256, 287
Eco105I TACGTA 1 cut(s) 352
Eco32I GATATC 1 cut(s) 318
EcoRV GATATC 1 cut(s) 318
FaeI CATG 2 cut(s) 109, 410
FaiI YATR 6 cut(s) 107, 138, 238, 402, 408, 443
FatI CATG 2 cut(s) 105, 406
FbaI TGATCA 1 cut(s) 108
Fnu4HI GCNGC 4 cut(s) 84, 216, 333, 498
FokI GGATG 1 cut(s) 458
Fsp4HI GCNGC 4 cut(s) 84, 216, 333, 498
GlaI GCGC 1 cut(s) 214
GluI GCNGC 4 cut(s) 84, 216, 333, 498
HaeIII GGCC 2 cut(s) 92, 377
HapII CCGG 1 cut(s) 378
HhaI GCGC 1 cut(s) 215
Hin1II CATG 2 cut(s) 109, 410
Hin6I GCGC 1 cut(s) 213
HinP1I GCGC 1 cut(s) 213
HincII GTYRAC 1 cut(s) 72
HindII GTYRAC 1 cut(s) 72
HinfI GANTC 1 cut(s) 40
HpaI GTTAAC 1 cut(s) 72
HpaII CCGG 1 cut(s) 378
Hpy166II GTNNAC 3 cut(s) 72, 193, 228
Hpy188I TCNGA 2 cut(s) 80, 390
Hpy188III TCNNGA 1 cut(s) 106
Hpy8I GTNNAC 3 cut(s) 72, 193, 228
HpyAV CCTTC 4 cut(s) 11, 338, 346, 457
HpyCH4III ACNGT 3 cut(s) 119, 365, 481
HpyCH4IV ACGT 1 cut(s) 351
HpyCH4V TGCA 4 cut(s) 86, 278, 406, 417
HpyF10VI GCNNNNNNNGC 9 cut(s) 89, 152, 203, 212, 248, 320, 329, 338, 497
HpySE526I ACGT 1 cut(s) 351
Hsp92II CATG 2 cut(s) 109, 410
HspAI GCGC 1 cut(s) 213
Ksp22I TGATCA 1 cut(s) 108
KspAI GTTAAC 1 cut(s) 72
Kzo9I GATC 2 cut(s) 108, 390
LmnI GCTCC 1 cut(s) 80
LpnPI CCDG 2 cut(s) 359, 391
Lsp1109I GCAGC 3 cut(s) 70, 319, 484
LweI GCATC 2 cut(s) 137, 150
MaeII ACGT 1 cut(s) 351
MaeIII GTNAC 3 cut(s) 347, 365, 424
MalI GATC 2 cut(s) 110, 392
MboI GATC 2 cut(s) 108, 390
MboII GAAGA 5 cut(s) 33, 36, 273, 274, 277
MnlI CCTC 8 cut(s) 103, 145, 181, 257, 446, 463, 473, 502
MseI TTAA 2 cut(s) 71, 485
MspA1I CMGCKG 1 cut(s) 497
MspI CCGG 1 cut(s) 378
MvnI CGCG 2 cut(s) 218, 341
MwoI GCNNNNNNNGC 9 cut(s) 89, 152, 203, 212, 248, 320, 329, 338, 497
NdeII GATC 2 cut(s) 108, 390
NlaIII CATG 2 cut(s) 109, 410
NmuCI GTSAC 1 cut(s) 424
PagI TCATGA 1 cut(s) 105
PfeI GAWTC 1 cut(s) 40
PkrI GCNGC 4 cut(s) 85, 217, 334, 499
Ppu21I YACGTR 1 cut(s) 352
RsaI GTAC 2 cut(s) 194, 362
RsaNI GTAC 2 cut(s) 193, 361
SaqAI TTAA 2 cut(s) 71, 485
SatI GCNGC 4 cut(s) 84, 216, 333, 498
Sau3AI GATC 2 cut(s) 108, 390
SetI ASST 7 cut(s) 85, 167, 185, 208, 325, 349, 354
SfaNI GCATC 2 cut(s) 137, 150
SnaBI TACGTA 1 cut(s) 352
SsiI CCGC 5 cut(s) 216, 256, 341, 396, 495
TaaI ACNGT 3 cut(s) 119, 365, 481
TaiI ACGT 1 cut(s) 354
TaqI TCGA 2 cut(s) 59, 95
TauI GCSGC 1 cut(s) 218
TfiI GAWTC 1 cut(s) 40
Tru1I TTAA 2 cut(s) 71, 485
Tru9I TTAA 2 cut(s) 71, 485
TseFI GTSAC 1 cut(s) 424
TseI GCWGC 3 cut(s) 83, 332, 497
Tsp45I GTSAC 1 cut(s) 424
TspDTI ATGAA 2 cut(s) 30, 460
TspGWI ACGGA 1 cut(s) 21
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.