FvH4_6g54070

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0003826
Physical Location & Seq
Forward (+)
39520800 .. 39522493
1694 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g54070.t1

Sequence Viewer

Length: 603 bp
ATGGCTACAGACTCGTCGTCGACGGTGGATAAAGTCGACAAGAACTTGTCCTCCCCTTCTCTCGATTCGACTAGTCTTCAACGGCGGAGGACGAGTTTCCTGATGGCTCAAGCTGGCATCAGAATCTTAGTTGTTGCCTTCACATTGATCGCAATCTCTGTCACGGTCACCAACCGCCAGTCCGTCGTCATCTTTGGAATGAACTTCCAAGCTCACTACACCTACTCGTCCGCCTTGAAGTTCTTGGTTGCTGCGGATGCCGTCTTGTGTTCCTTCTCAGCGGTGTCGTTGGTCTTTATCTACTCTTTAAGCCGTTCTGGGTCGACAAATTCACCTCTCAAGAAGTACTTCTTCCTCTTCTTGCTTGATACGGTGATGATGGTGTTGATAATAGCTGGATGTGCAGCGGCGACTGCAATAGGGTACGTGGGGAAGTACGGGGAAGAACACATGACTTGGCAGGCAACTTGTGGATATGTGTCCAAGTTCTGCAACAGAATGTCTATCTCTCTTGCATTTTCGTACTTGGCCTTCGTTGCCTCCTTGCTTCTCAATTTCATGTCTGCTCATGCTCTCATCTATCGCCCCATTATCAAGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0048037 GO:0051536 GO:0051540
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

201

Amino Acids

21.91

Weight (kDa)

9.56

Isoelectric Point (pI)

30.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 33 - 180 3.6e-35 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 20, 36, 323
AciI CCGC 6 cut(s) 85, 175, 231, 254, 281, 407
AcsI RAATTY 1 cut(s) 328
AfaI GTAC 4 cut(s) 347, 425, 437, 524
AgsI TTSAA 2 cut(s) 80, 238
AhdI GACNNNNNGTC 1 cut(s) 16
AhlI ACTAGT 1 cut(s) 71
AloI GAACNNNNNNTCC 2 cut(s) 35, 67
AluBI AGCT 3 cut(s) 113, 212, 395
AluI AGCT 3 cut(s) 113, 212, 395
AoxI GGCC 1 cut(s) 528
ApeKI GCWGC 2 cut(s) 251, 404
ApoI RAATTY 1 cut(s) 328
Asp700I GAANNNNTTC 1 cut(s) 347
AsuHPI GGTGA 3 cut(s) 160, 324, 385
BarI GAAGNNNNNNTAC 2 cut(s) 515, 547
BbsI GAAGAC 1 cut(s) 68
BbvI GCAGC 2 cut(s) 238, 416
BccI CCATC 2 cut(s) 97, 373
BceAI ACGGC 3 cut(s) 98, 245, 297
BcuI ACTAGT 1 cut(s) 71
BfaI CTAG 1 cut(s) 72
BfmI CTRYAG 1 cut(s) 6
BisI GCNGC 3 cut(s) 252, 405, 408
BlsI GCNGC 3 cut(s) 253, 406, 409
BmcAI AGTACT 1 cut(s) 347
BmeRI GACNNNNNGTC 1 cut(s) 16
BmsI GCATC 2 cut(s) 126, 247
BpiI GAAGAC 1 cut(s) 68
BpuEI CTTGAG 2 cut(s) 93, 323
BsaAI YACGTR 1 cut(s) 427
BsaBI GATNNNNATC 1 cut(s) 152
BsaXI ACNNNNNCTCC 4 cut(s) 35, 65, 79, 109
Bse1I ACTGG 1 cut(s) 178
Bse8I GATNNNNATC 1 cut(s) 152
BseGI GGATG 2 cut(s) 262, 404
BseJI GATNNNNATC 1 cut(s) 152
BseMII CTCAG 1 cut(s) 291
BseNI ACTGG 1 cut(s) 178
BseXI GCAGC 2 cut(s) 238, 416
BsgI GTGCAG 1 cut(s) 423
BshFI GGCC 1 cut(s) 530
BsnI GGCC 1 cut(s) 530
Bsp143I GATC 1 cut(s) 147
BspACI CCGC 6 cut(s) 85, 175, 231, 254, 281, 407
BspANI GGCC 1 cut(s) 530
BspCNI CTCAG 1 cut(s) 290
BsrI ACTGG 1 cut(s) 178
BssMI GATC 1 cut(s) 147
Bst4CI ACNGT 3 cut(s) 25, 166, 373
Bst6I CTCTTC 1 cut(s) 362
BstBAI YACGTR 1 cut(s) 427
BstC8I GCNNGC 2 cut(s) 115, 462
BstDEI CTNAG 2 cut(s) 127, 277
BstEII GGTNACC 1 cut(s) 166
BstF5I GGATG 2 cut(s) 262, 404
BstKTI GATC 1 cut(s) 150
BstMBI GATC 1 cut(s) 147
BstMWI GCNNNNNNNGC 4 cut(s) 257, 401, 413, 536
BstPI GGTNACC 1 cut(s) 166
BstSFI CTRYAG 1 cut(s) 6
BstV1I GCAGC 2 cut(s) 238, 416
BstV2I GAAGAC 1 cut(s) 68
BsuRI GGCC 1 cut(s) 530
BtsCI GGATG 2 cut(s) 262, 404
Cac8I GCNNGC 2 cut(s) 115, 462
Csp6I GTAC 4 cut(s) 346, 424, 436, 523
CviAII CATG 3 cut(s) 451, 559, 569
CviJI RGCY 7 cut(s) 5, 107, 113, 212, 312, 395, 530
CviKI_1 RGCY 7 cut(s) 5, 107, 113, 212, 312, 395, 530
CviQI GTAC 4 cut(s) 346, 424, 436, 523
DdeI CTNAG 2 cut(s) 127, 277
DpnI GATC 1 cut(s) 149
DpnII GATC 1 cut(s) 147
DriI GACNNNNNGTC 1 cut(s) 16
Eam1104I CTCTTC 1 cut(s) 362
Eam1105I GACNNNNNGTC 1 cut(s) 16
EarI CTCTTC 1 cut(s) 362
EciI GGCGGA 2 cut(s) 100, 220
Eco91I GGTNACC 1 cut(s) 166
EcoO65I GGTNACC 1 cut(s) 166
FaeI CATG 3 cut(s) 454, 562, 572
FaiI YATR 4 cut(s) 452, 477, 560, 570
FalI AAGNNNNNCTT 4 cut(s) 332, 364, 335, 367
FatI CATG 3 cut(s) 450, 558, 568
FblI GTMKAC 3 cut(s) 20, 36, 323
Fnu4HI GCNGC 3 cut(s) 252, 405, 408
FokI GGATG 2 cut(s) 269, 411
Fsp4HI GCNGC 3 cut(s) 252, 405, 408
FspBI CTAG 1 cut(s) 72
GluI GCNGC 3 cut(s) 252, 405, 408
HaeIII GGCC 1 cut(s) 530
Hin1II CATG 3 cut(s) 454, 562, 572
HincII GTYRAC 3 cut(s) 21, 37, 324
HindII GTYRAC 3 cut(s) 21, 37, 324
HinfI GANTC 3 cut(s) 11, 65, 123
HphI GGTGA 3 cut(s) 160, 324, 385
Hpy166II GTNNAC 3 cut(s) 21, 37, 324
Hpy188I TCNGA 1 cut(s) 122
Hpy188III TCNNGA 4 cut(s) 62, 100, 340, 595
Hpy8I GTNNAC 3 cut(s) 21, 37, 324
Hpy99I CGWCG 4 cut(s) 19, 22, 25, 188
HpyAV CCTTC 4 cut(s) 66, 148, 283, 541
HpyCH4III ACNGT 3 cut(s) 25, 166, 373
HpyCH4IV ACGT 1 cut(s) 426
HpyCH4V TGCA 4 cut(s) 404, 416, 492, 515
HpyF10VI GCNNNNNNNGC 4 cut(s) 257, 401, 413, 536
HpyF3I CTNAG 2 cut(s) 127, 277
HpySE526I ACGT 1 cut(s) 426
Hsp92II CATG 3 cut(s) 454, 562, 572
Kzo9I GATC 1 cut(s) 147
LpnPI CCDG 6 cut(s) 99, 113, 191, 303, 381, 446
Lsp1109I GCAGC 2 cut(s) 238, 416
LweI GCATC 2 cut(s) 126, 247
MaeI CTAG 1 cut(s) 72
MaeII ACGT 1 cut(s) 426
MaeIII GTNAC 2 cut(s) 160, 166
MalI GATC 1 cut(s) 149
MboI GATC 1 cut(s) 147
MboII GAAGA 4 cut(s) 68, 343, 349, 455
MluCI AATT 2 cut(s) 328, 553
MlyI GAGTC 1 cut(s) 5
MnlI CCTC 5 cut(s) 61, 81, 345, 365, 550
MroXI GAANNNNTTC 1 cut(s) 347
MseI TTAA 1 cut(s) 308
MspA1I CMGCKG 2 cut(s) 281, 407
MwoI GCNNNNNNNGC 4 cut(s) 257, 401, 413, 536
NdeII GATC 1 cut(s) 147
NlaIII CATG 3 cut(s) 454, 562, 572
NmuCI GTSAC 2 cut(s) 160, 166
PdmI GAANNNNTTC 1 cut(s) 347
PfeI GAWTC 2 cut(s) 65, 123
PkrI GCNGC 3 cut(s) 253, 406, 409
PleI GAGTC 1 cut(s) 5
PpsI GAGTC 1 cut(s) 5
Ppu21I YACGTR 1 cut(s) 427
PspEI GGTNACC 1 cut(s) 166
RsaI GTAC 4 cut(s) 347, 425, 437, 524
RsaNI GTAC 4 cut(s) 346, 424, 436, 523
SalI GTCGAC 3 cut(s) 19, 35, 322
SaqAI TTAA 1 cut(s) 308
SatI GCNGC 3 cut(s) 252, 405, 408
Sau3AI GATC 1 cut(s) 147
ScaI AGTACT 1 cut(s) 347
SchI GAGTC 1 cut(s) 5
SetI ASST 6 cut(s) 115, 214, 224, 337, 397, 429
SfaNI GCATC 2 cut(s) 126, 247
SfcI CTRYAG 1 cut(s) 6
SgrDI CGTCGACG 1 cut(s) 19
SmlI CTYRAG 2 cut(s) 108, 338
SmoI CTYRAG 2 cut(s) 108, 338
SpeI ACTAGT 1 cut(s) 71
Sse9I AATT 2 cut(s) 328, 553
SsiI CCGC 6 cut(s) 85, 175, 231, 254, 281, 407
SspMI CTAG 1 cut(s) 72
TaaI ACNGT 3 cut(s) 25, 166, 373
TaiI ACGT 1 cut(s) 429
TaqI TCGA 5 cut(s) 20, 36, 63, 68, 323
TasI AATT 2 cut(s) 328, 553
TatI WGTACW 1 cut(s) 345
TauI GCSGC 1 cut(s) 410
TfiI GAWTC 2 cut(s) 65, 123
Tru1I TTAA 1 cut(s) 308
Tru9I TTAA 1 cut(s) 308
TseFI GTSAC 2 cut(s) 160, 166
TseI GCWGC 2 cut(s) 251, 404
Tsp45I GTSAC 2 cut(s) 160, 166
TspDTI ATGAA 2 cut(s) 215, 547
TspGWI ACGGA 1 cut(s) 172
XapI RAATTY 1 cut(s) 328
XmiI GTMKAC 3 cut(s) 20, 36, 323
XmnI GAANNNNTTC 1 cut(s) 347
XspI CTAG 1 cut(s) 72
ZrmI AGTACT 1 cut(s) 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.