FvH4_5g09090

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0003826
Physical Location & Seq
Forward (+)
5248543 .. 5249668
1126 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g09090.t1

Sequence Viewer

Length: 549 bp
ATGGCAACCATAGAAGCCAAGCTTCAGCCAAACCCTCCTGTGATGAAAAGCCAAAAGCTTTTTCTTGGTGTTCAAATTTGGTTGAGGACTTTTGCCATGGCCACTACATTAGCTGCAACTTGGATCACAATCACAAGCAAACAGTCCACTGAGATTGTTGGCATAACCTTTGATGCTAGATATAGCTACTCCTCTGCCTTCAAGTTCTTGGCTTTTGCAAATGCTGTTGTGAGTGCTTGTTGTTTGGTGTCCTTGCTCCTTGTTTTCCTTCTCCATCTTCAAGGCTCAAATCCCAATCACTACTTCTTCTTGTTCCTCCATGATTTGTTCATTATGTGCTTGGTTCTTGCTGGATGCGCGGCTGCAACTGCGATAGGGTTTGTGGGGCGGTATGGGAACAGCCACACTGGTTGGAGTCCAATTTGTGATCACTTTGATAAGTTCTGTCACAGAGTGTCAATATCTGTGATTCTCTCCTACTTATCTCTCATAATTTTGCTGATGCTTACTGTTGGCTCAGCAATCAAGTCTAGACAGATTCGGGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0048037 GO:0051536 GO:0051540
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

183

Amino Acids

20.12

Weight (kDa)

9.3

Isoelectric Point (pI)

40.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 23 - 164 1e-35 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 359
AciI CCGC 2 cut(s) 359, 388
AclWI GGATC 1 cut(s) 131
AcoI YGGCCR 1 cut(s) 99
AcsI RAATTY 1 cut(s) 75
AcuI CTGAAG 1 cut(s) 8
AdeI CACNNNGTG 1 cut(s) 454
AgsI TTSAA 3 cut(s) 74, 202, 281
AjuI GAANNNNNNNTTGG 4 cut(s) 45, 77, 287, 319
AluBI AGCT 4 cut(s) 22, 58, 113, 186
AluI AGCT 4 cut(s) 22, 58, 113, 186
AlwI GGATC 1 cut(s) 131
AoxI GGCC 1 cut(s) 99
ApeKI GCWGC 2 cut(s) 113, 362
ApoI RAATTY 1 cut(s) 75
AspLEI GCGC 1 cut(s) 359
BalI TGGCCA 1 cut(s) 101
BbvI GCAGC 2 cut(s) 100, 349
BccI CCATC 1 cut(s) 282
BclI TGATCA 1 cut(s) 427
BfaI CTAG 2 cut(s) 177, 531
BisI GCNGC 3 cut(s) 114, 360, 363
BlpI GCTNAGC 1 cut(s) 517
BlsI GCNGC 3 cut(s) 115, 361, 364
BmsI GCATC 3 cut(s) 163, 344, 492
Bpu1102I GCTNAGC 1 cut(s) 517
BsaBI GATNNNNATC 1 cut(s) 128
BsaJI CCNNGG 1 cut(s) 96
Bse1I ACTGG 1 cut(s) 412
Bse8I GATNNNNATC 1 cut(s) 128
BseDI CCNNGG 1 cut(s) 96
BseGI GGATG 1 cut(s) 359
BseJI GATNNNNATC 1 cut(s) 128
BseMII CTCAG 2 cut(s) 141, 531
BseNI ACTGG 1 cut(s) 412
BseRI GAGGAG 1 cut(s) 181
BseXI GCAGC 2 cut(s) 100, 349
Bsh1236I CGCG 1 cut(s) 359
BshFI GGCC 1 cut(s) 101
BsnI GGCC 1 cut(s) 101
Bsp143I GATC 2 cut(s) 123, 427
Bsp1720I GCTNAGC 1 cut(s) 517
Bsp19I CCATGG 1 cut(s) 96
BspACI CCGC 2 cut(s) 359, 388
BspANI GGCC 1 cut(s) 101
BspCNI CTCAG 2 cut(s) 142, 530
BspFNI CGCG 1 cut(s) 359
BspPI GGATC 1 cut(s) 131
BsrI ACTGG 1 cut(s) 412
BssECI CCNNGG 1 cut(s) 96
BssMI GATC 2 cut(s) 123, 427
BssT1I CCWWGG 1 cut(s) 96
Bst4CI ACNGT 2 cut(s) 144, 511
BstDEI CTNAG 2 cut(s) 150, 517
BstDSI CCRYGG 1 cut(s) 96
BstF5I GGATG 1 cut(s) 359
BstFNI CGCG 1 cut(s) 359
BstHHI GCGC 1 cut(s) 359
BstKTI GATC 2 cut(s) 126, 430
BstMBI GATC 2 cut(s) 123, 427
BstMWI GCNNNNNNNGC 2 cut(s) 356, 368
BstUI CGCG 1 cut(s) 359
BstV1I GCAGC 2 cut(s) 100, 349
BsuRI GGCC 1 cut(s) 101
BtgI CCRYGG 1 cut(s) 96
BtsCI GGATG 1 cut(s) 359
BtsIMutI CAGTG 2 cut(s) 147, 405
CfoI GCGC 1 cut(s) 359
CspCI CAANNNNNGTGG 4 cut(s) 136, 171, 392, 427
CviAII CATG 2 cut(s) 97, 320
DdeI CTNAG 2 cut(s) 150, 517
DpnI GATC 2 cut(s) 125, 429
DpnII GATC 2 cut(s) 123, 427
DraIII CACNNNGTG 1 cut(s) 454
EaeI YGGCCR 1 cut(s) 99
Eco130I CCWWGG 1 cut(s) 96
Eco57I CTGAAG 1 cut(s) 8
EcoT14I CCWWGG 1 cut(s) 96
ErhI CCWWGG 1 cut(s) 96
FaeI CATG 2 cut(s) 100, 323
FaiI YATR 8 cut(s) 11, 98, 164, 183, 321, 335, 393, 491
FalI AAGNNNNNCTT 1 cut(s) 38
FatI CATG 2 cut(s) 96, 319
FbaI TGATCA 1 cut(s) 427
Fnu4HI GCNGC 3 cut(s) 114, 360, 363
FokI GGATG 1 cut(s) 366
Fsp4HI GCNGC 3 cut(s) 114, 360, 363
FspBI CTAG 2 cut(s) 177, 531
GlaI GCGC 1 cut(s) 358
GluI GCNGC 3 cut(s) 114, 360, 363
HaeIII GGCC 1 cut(s) 101
HhaI GCGC 1 cut(s) 359
Hin1II CATG 2 cut(s) 100, 323
Hin6I GCGC 1 cut(s) 357
HinP1I GCGC 1 cut(s) 357
HindIII AAGCTT 2 cut(s) 20, 56
HinfI GANTC 3 cut(s) 415, 469, 538
Hpy166II GTNNAC 1 cut(s) 147
Hpy188III TCNNGA 1 cut(s) 531
Hpy8I GTNNAC 1 cut(s) 147
HpyAV CCTTC 2 cut(s) 208, 278
HpyCH4III ACNGT 2 cut(s) 144, 511
HpyCH4V TGCA 3 cut(s) 116, 218, 365
HpyF10VI GCNNNNNNNGC 2 cut(s) 356, 368
HpyF3I CTNAG 2 cut(s) 150, 517
Hsp92II CATG 2 cut(s) 100, 323
HspAI GCGC 1 cut(s) 357
Ksp22I TGATCA 1 cut(s) 427
Kzo9I GATC 2 cut(s) 123, 427
LmnI GCTCC 1 cut(s) 261
LpnPI CCDG 3 cut(s) 51, 336, 393
Lsp1109I GCAGC 2 cut(s) 100, 349
LweI GCATC 3 cut(s) 163, 344, 492
MaeI CTAG 2 cut(s) 177, 531
MaeIII GTNAC 1 cut(s) 446
MalI GATC 2 cut(s) 125, 429
MboI GATC 2 cut(s) 123, 427
MboII GAAGA 2 cut(s) 269, 298
MlsI TGGCCA 1 cut(s) 101
MluCI AATT 3 cut(s) 75, 420, 492
MluNI TGGCCA 1 cut(s) 101
MlyI GAGTC 1 cut(s) 424
MmeI TCCRAC 1 cut(s) 392
MnlI CCTC 4 cut(s) 45, 78, 202, 326
Mox20I TGGCCA 1 cut(s) 101
MscI TGGCCA 1 cut(s) 101
Msp20I TGGCCA 1 cut(s) 101
MvnI CGCG 1 cut(s) 359
MwoI GCNNNNNNNGC 2 cut(s) 356, 368
NcoI CCATGG 1 cut(s) 96
NdeII GATC 2 cut(s) 123, 427
NlaIII CATG 2 cut(s) 100, 323
NmuCI GTSAC 1 cut(s) 446
PfeI GAWTC 2 cut(s) 469, 538
PkrI GCNGC 3 cut(s) 115, 361, 364
PleI GAGTC 1 cut(s) 423
PpsI GAGTC 1 cut(s) 423
SatI GCNGC 3 cut(s) 114, 360, 363
Sau3AI GATC 2 cut(s) 123, 427
SchI GAGTC 1 cut(s) 424
SetI ASST 5 cut(s) 24, 60, 115, 170, 188
SfaNI GCATC 3 cut(s) 163, 344, 492
Sse9I AATT 3 cut(s) 75, 420, 492
SsiI CCGC 2 cut(s) 359, 388
SspMI CTAG 2 cut(s) 177, 531
StyI CCWWGG 1 cut(s) 96
TaaI ACNGT 2 cut(s) 144, 511
TasI AATT 3 cut(s) 75, 420, 492
TauI GCSGC 1 cut(s) 362
TfiI GAWTC 2 cut(s) 469, 538
TscAI CASTG 2 cut(s) 154, 412
TseFI GTSAC 1 cut(s) 446
TseI GCWGC 2 cut(s) 113, 362
Tsp45I GTSAC 1 cut(s) 446
TspDTI ATGAA 2 cut(s) 59, 319
TspRI CASTG 2 cut(s) 154, 412
XapI RAATTY 1 cut(s) 75
XbaI TCTAGA 1 cut(s) 530
XspI CTAG 2 cut(s) 177, 531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.