Rroxscaffold_2G00076640

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0003826
Physical Location & Seq
Reverse (-)
391272 .. 392648
1377 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00076640.1

Sequence Viewer

Length: 591 bp
ATGGCTTCAGAGTCTTCGACGGTGGCTAAAGACGAGAAGAACTTGTCCTCTTTTGTTGATTCGAGCAGTCAACGGCGGAAAACGAGTTTCTTGATGGCTCAAGCGGGTCTCAGAATCTTAGTTGTTGCCTTCACATTGGTTGCAATCTCTGTAATGGTCACCAACCGCCAGTCCGTCGTCATCTTTGGATTCAACTTCCAAGCACGATACTCCTACTCGTCTGCCTTCAAGTTCTTGGTTGCTGCGGATGCTGTGTTGTGTTCCTTCTCAGCACTGTCGTTGGTCTTCATCTACATCTTAAGCCGTTCTGGGACAACTTCGCCTCTCAAGAAGTACTTCCTCCTCTTCTTGCTTGATACGGTGATGATGGTGTTAATAATAGCTGGATGTGCAGCTGCGACTGCAATAGGGTACTTGGGAAAGTATGGGGAAGAACACATGACTTGGCATGCAACTTGTGGATATGTGTCCAAGTTCTGCAACAGAATGTCCATCTCTCTTGCATTTTCATACTTGTCCTTCTTTGCCAGCTTGCTTCTCAATCTCATGTCGGCTCATGCTCTCATCTATCGCCCTATTATCAAGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0048037 GO:0051536 GO:0051540
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

196

Amino Acids

21.62

Weight (kDa)

9.56

Isoelectric Point (pI)

31.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 30 - 176 2.5e-37 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 76, 104, 166, 245
AfaI GTAC 2 cut(s) 335, 413
AflII CTTAAG 1 cut(s) 298
AgsI TTSAA 2 cut(s) 193, 229
AluBI AGCT 3 cut(s) 383, 395, 531
AluI AGCT 3 cut(s) 383, 395, 531
Alw26I GTCTC 1 cut(s) 113
ApeKI GCWGC 3 cut(s) 242, 392, 395
Asp700I GAANNNNTTC 1 cut(s) 335
AsuHPI GGTGA 2 cut(s) 151, 373
BbsI GAAGAC 2 cut(s) 6, 277
BbvI GCAGC 3 cut(s) 229, 382, 404
BccI CCATC 3 cut(s) 88, 361, 500
BceAI ACGGC 2 cut(s) 89, 288
BcoDI GTCTC 1 cut(s) 113
BfrI CTTAAG 1 cut(s) 298
BisI GCNGC 3 cut(s) 243, 393, 396
BlsI GCNGC 3 cut(s) 244, 394, 397
BmcAI AGTACT 1 cut(s) 335
BmsI GCATC 1 cut(s) 238
BpiI GAAGAC 2 cut(s) 6, 277
BpuEI CTTGAG 2 cut(s) 84, 311
BsaI GGTCTC 1 cut(s) 113
Bse1I ACTGG 1 cut(s) 169
BseGI GGATG 2 cut(s) 253, 392
BseMII CTCAG 2 cut(s) 124, 282
BseNI ACTGG 1 cut(s) 169
BseRI GAGGAG 1 cut(s) 332
BseXI GCAGC 3 cut(s) 229, 382, 404
BsgI GTGCAG 1 cut(s) 411
BslFI GGGAC 1 cut(s) 325
BsmAI GTCTC 1 cut(s) 113
BsmFI GGGAC 1 cut(s) 325
Bso31I GGTCTC 1 cut(s) 113
BspACI CCGC 4 cut(s) 76, 104, 166, 245
BspCNI CTCAG 2 cut(s) 123, 281
BspTI CTTAAG 1 cut(s) 298
BspTNI GGTCTC 1 cut(s) 113
BsrI ACTGG 1 cut(s) 169
Bst4CI ACNGT 3 cut(s) 22, 276, 361
Bst6I CTCTTC 1 cut(s) 350
BstAFI CTTAAG 1 cut(s) 298
BstC8I GCNNGC 3 cut(s) 450, 529, 533
BstDEI CTNAG 3 cut(s) 110, 118, 268
BstEII GGTNACC 1 cut(s) 157
BstF5I GGATG 2 cut(s) 253, 392
BstMAI GTCTC 1 cut(s) 113
BstMWI GCNNNNNNNGC 3 cut(s) 248, 389, 401
BstNSI RCATGY 1 cut(s) 452
BstPI GGTNACC 1 cut(s) 157
BstV1I GCAGC 3 cut(s) 229, 382, 404
BstV2I GAAGAC 2 cut(s) 6, 277
BtsCI GGATG 2 cut(s) 253, 392
BtsIMutI CAGTG 1 cut(s) 272
Cac8I GCNNGC 3 cut(s) 450, 529, 533
Csp6I GTAC 2 cut(s) 334, 412
CviAII CATG 4 cut(s) 439, 449, 547, 557
CviJI RGCY 8 cut(s) 5, 26, 98, 303, 383, 395, 531, 554
CviKI_1 RGCY 8 cut(s) 5, 26, 98, 303, 383, 395, 531, 554
CviQI GTAC 2 cut(s) 334, 412
DdeI CTNAG 3 cut(s) 110, 118, 268
Eam1104I CTCTTC 1 cut(s) 350
EarI CTCTTC 1 cut(s) 350
EciI GGCGGA 1 cut(s) 91
Eco31I GGTCTC 1 cut(s) 113
Eco91I GGTNACC 1 cut(s) 157
EcoO65I GGTNACC 1 cut(s) 157
FaeI CATG 4 cut(s) 442, 452, 550, 560
FaiI YATR 7 cut(s) 426, 440, 450, 465, 511, 548, 558
FalI AAGNNNNNCTT 2 cut(s) 320, 352
FaqI GGGAC 1 cut(s) 325
FatI CATG 4 cut(s) 438, 448, 546, 556
FauI CCCGC 1 cut(s) 97
Fnu4HI GCNGC 3 cut(s) 243, 393, 396
FokI GGATG 2 cut(s) 260, 399
Fsp4HI GCNGC 3 cut(s) 243, 393, 396
GluI GCNGC 3 cut(s) 243, 393, 396
Hin1II CATG 4 cut(s) 442, 452, 550, 560
HincII GTYRAC 1 cut(s) 71
HindII GTYRAC 1 cut(s) 71
HinfI GANTC 4 cut(s) 11, 59, 114, 189
HphI GGTGA 2 cut(s) 151, 373
Hpy166II GTNNAC 1 cut(s) 71
Hpy188I TCNGA 2 cut(s) 10, 113
Hpy188III TCNNGA 3 cut(s) 91, 328, 583
Hpy8I GTNNAC 1 cut(s) 71
Hpy99I CGWCG 2 cut(s) 22, 179
HpyAV CCTTC 4 cut(s) 139, 235, 274, 529
HpyCH4III ACNGT 3 cut(s) 22, 276, 361
HpyCH4V TGCA 6 cut(s) 143, 392, 404, 452, 480, 503
HpyF10VI GCNNNNNNNGC 3 cut(s) 248, 389, 401
HpyF3I CTNAG 3 cut(s) 110, 118, 268
Hsp92II CATG 4 cut(s) 442, 452, 550, 560
LpnPI CCDG 4 cut(s) 182, 294, 369, 541
Lsp1109I GCAGC 3 cut(s) 229, 382, 404
LweI GCATC 1 cut(s) 238
MaeIII GTNAC 1 cut(s) 157
MboII GAAGA 5 cut(s) 6, 49, 277, 337, 443
MlyI GAGTC 1 cut(s) 20
MnlI CCTC 4 cut(s) 58, 333, 350, 353
MroXI GAANNNNTTC 1 cut(s) 335
MseI TTAA 2 cut(s) 299, 374
MspA1I CMGCKG 1 cut(s) 395
MspCI CTTAAG 1 cut(s) 298
MwoI GCNNNNNNNGC 3 cut(s) 248, 389, 401
NlaIII CATG 4 cut(s) 442, 452, 550, 560
NmuCI GTSAC 1 cut(s) 157
NspI RCATGY 1 cut(s) 452
PaeI GCATGC 1 cut(s) 452
PdmI GAANNNNTTC 1 cut(s) 335
PfeI GAWTC 3 cut(s) 59, 114, 189
PkrI GCNGC 3 cut(s) 244, 394, 397
PleI GAGTC 1 cut(s) 19
PpsI GAGTC 1 cut(s) 19
PspEI GGTNACC 1 cut(s) 157
PvuII CAGCTG 1 cut(s) 395
RsaI GTAC 2 cut(s) 335, 413
RsaNI GTAC 2 cut(s) 334, 412
SaqAI TTAA 2 cut(s) 299, 374
SatI GCNGC 3 cut(s) 243, 393, 396
ScaI AGTACT 1 cut(s) 335
SchI GAGTC 1 cut(s) 20
SetI ASST 3 cut(s) 385, 397, 533
SfaNI GCATC 1 cut(s) 238
SmlI CTYRAG 3 cut(s) 99, 298, 326
SmoI CTYRAG 3 cut(s) 99, 298, 326
SphI GCATGC 1 cut(s) 452
SsiI CCGC 4 cut(s) 76, 104, 166, 245
TaaI ACNGT 3 cut(s) 22, 276, 361
TaqI TCGA 2 cut(s) 17, 62
TatI WGTACW 1 cut(s) 333
TfiI GAWTC 3 cut(s) 59, 114, 189
Tru1I TTAA 2 cut(s) 299, 374
Tru9I TTAA 2 cut(s) 299, 374
TscAI CASTG 1 cut(s) 279
TseFI GTSAC 1 cut(s) 157
TseI GCWGC 3 cut(s) 242, 392, 395
Tsp45I GTSAC 1 cut(s) 157
TspDTI ATGAA 2 cut(s) 277, 498
TspGWI ACGGA 1 cut(s) 163
TspRI CASTG 1 cut(s) 279
Vha464I CTTAAG 1 cut(s) 298
XceI RCATGY 1 cut(s) 452
XmnI GAANNNNTTC 1 cut(s) 335
ZrmI AGTACT 1 cut(s) 335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.