RLG00000004632

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0003826
Physical Location & Seq
Forward (+)
60970611 .. 60971400
790 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004632

Sequence Viewer

Length: 552 bp
ATGGCAACCATTGAAGCCAAGGCGTTTCAGCCAAACCCACCTGTGAGAACCCAAAAGCTTTTTCTTGGTGCCCAAATTTGCTTGAGGACTGTTGCCATGGCTACTACATTAGCTGCAATATGGATCATGGTCACAAGCAAACAGTCCACAGAGATTGTTGGCATAGCCTTTGATGCTAGATATAGCTACTCATCTGCCTTCAAGTTCTTGGCTTTTGCAAATGCTGTTGTCAGTGCCTGTTGTTTAGTGTCCTTGCTCCTTGTTTTCCTTGTCCATCTCCAAGGCTCAAATCCCAATCACCACTTCTTCTTGTTCCTCCATGATTTGTTCATCATGTGCTTGGTGCTTGCTGGATGCGCGGCTGCAACGGCGATAGGGTTCGTGGCGCGGTATGGGAACAGCCACACCGGTTGGACTCCGATTTGTGATCACTTTGGGAAGTTCTGTCACAGAGTGTCAATATCTGTGATTCTGTCCTACTTGTCCCTCATAATTTTGCTGATGCTTACTGTCAGCTCAGCAATCAAGTCTAGACAGATTCCGGTAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0048037 GO:0051536 GO:0051540
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

184

Amino Acids

19.97

Weight (kDa)

9.25

Isoelectric Point (pI)

32.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 23 - 164 2.8e-37 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 68
AccII CGCG 2 cut(s) 359, 388
AciI CCGC 2 cut(s) 359, 388
AclWI GGATC 1 cut(s) 131
AcsI RAATTY 1 cut(s) 75
AdeI CACNNNGTG 1 cut(s) 454
AgeI ACCGGT 1 cut(s) 407
AgsI TTSAA 2 cut(s) 14, 202
AjuI GAANNNNNNNTTGG 4 cut(s) 45, 77, 287, 319
AluBI AGCT 4 cut(s) 58, 113, 186, 516
AluI AGCT 4 cut(s) 58, 113, 186, 516
AlwI GGATC 1 cut(s) 131
AlwNI CAGNNNCTG 1 cut(s) 237
ApeKI GCWGC 2 cut(s) 113, 362
ApoI RAATTY 1 cut(s) 75
AsiGI ACCGGT 1 cut(s) 407
AspLEI GCGC 2 cut(s) 359, 388
AsuHPI GGTGA 1 cut(s) 290
BaeGI GKGCMC 1 cut(s) 73
BanI GGYRCC 1 cut(s) 68
BbvI GCAGC 2 cut(s) 100, 349
BccI CCATC 1 cut(s) 282
BceAI ACGGC 1 cut(s) 384
BclI TGATCA 1 cut(s) 427
BfaI CTAG 2 cut(s) 177, 531
BisI GCNGC 3 cut(s) 114, 360, 363
BlpI GCTNAGC 1 cut(s) 517
BlsI GCNGC 3 cut(s) 115, 361, 364
BmiI GGNNCC 1 cut(s) 70
BmsI GCATC 3 cut(s) 163, 344, 492
Bpu1102I GCTNAGC 1 cut(s) 517
BpuEI CTTGAG 1 cut(s) 103
BsaJI CCNNGG 3 cut(s) 18, 96, 280
BsaWI WCCGGW 2 cut(s) 407, 541
Bse118I RCCGGY 1 cut(s) 407
BseDI CCNNGG 3 cut(s) 18, 96, 280
BseGI GGATG 1 cut(s) 359
BseMII CTCAG 1 cut(s) 531
BseSI GKGCMC 1 cut(s) 73
BseXI GCAGC 2 cut(s) 100, 349
Bsh1236I CGCG 2 cut(s) 359, 388
BshNI GGYRCC 1 cut(s) 68
BshTI ACCGGT 1 cut(s) 407
BsiSI CCGG 2 cut(s) 408, 542
BslFI GGGAC 1 cut(s) 469
BsmFI GGGAC 1 cut(s) 469
Bsp1286I GDGCHC 1 cut(s) 73
Bsp143I GATC 2 cut(s) 123, 427
Bsp1720I GCTNAGC 1 cut(s) 517
Bsp19I CCATGG 1 cut(s) 96
BspACI CCGC 2 cut(s) 359, 388
BspCNI CTCAG 1 cut(s) 530
BspFNI CGCG 2 cut(s) 359, 388
BspLI GGNNCC 1 cut(s) 70
BspPI GGATC 1 cut(s) 131
BspT107I GGYRCC 1 cut(s) 68
BsrFI RCCGGY 1 cut(s) 407
BssAI RCCGGY 1 cut(s) 407
BssECI CCNNGG 3 cut(s) 18, 96, 280
BssMI GATC 2 cut(s) 123, 427
BssT1I CCWWGG 3 cut(s) 18, 96, 280
Bst4CI ACNGT 3 cut(s) 91, 144, 511
BstC8I GCNNGC 1 cut(s) 348
BstDEI CTNAG 1 cut(s) 517
BstDSI CCRYGG 1 cut(s) 96
BstF5I GGATG 1 cut(s) 359
BstFNI CGCG 2 cut(s) 359, 388
BstHHI GCGC 2 cut(s) 359, 388
BstKTI GATC 2 cut(s) 126, 430
BstMBI GATC 2 cut(s) 123, 427
BstMWI GCNNNNNNNGC 3 cut(s) 173, 356, 368
BstSLI GKGCMC 1 cut(s) 73
BstUI CGCG 2 cut(s) 359, 388
BstV1I GCAGC 2 cut(s) 100, 349
BtgI CCRYGG 1 cut(s) 96
BtsCI GGATG 1 cut(s) 359
BtsIMutI CAGTG 1 cut(s) 238
Cac8I GCNNGC 1 cut(s) 348
CaiI CAGNNNCTG 1 cut(s) 237
CfoI GCGC 2 cut(s) 359, 388
Cfr10I RCCGGY 1 cut(s) 407
CspAI ACCGGT 1 cut(s) 407
CspCI CAANNNNNGTGG 4 cut(s) 136, 171, 392, 427
CviAII CATG 4 cut(s) 97, 127, 320, 334
DdeI CTNAG 1 cut(s) 517
DpnI GATC 2 cut(s) 125, 429
DpnII GATC 2 cut(s) 123, 427
DraIII CACNNNGTG 1 cut(s) 454
Eco130I CCWWGG 3 cut(s) 18, 96, 280
EcoT14I CCWWGG 3 cut(s) 18, 96, 280
ErhI CCWWGG 3 cut(s) 18, 96, 280
FaeI CATG 4 cut(s) 100, 130, 323, 337
FaiI YATR 9 cut(s) 98, 121, 128, 164, 183, 321, 335, 393, 491
FaqI GGGAC 1 cut(s) 469
FatI CATG 4 cut(s) 96, 126, 319, 333
FbaI TGATCA 1 cut(s) 427
Fnu4HI GCNGC 3 cut(s) 114, 360, 363
FokI GGATG 1 cut(s) 366
Fsp4HI GCNGC 3 cut(s) 114, 360, 363
FspBI CTAG 2 cut(s) 177, 531
GlaI GCGC 2 cut(s) 358, 387
GluI GCNGC 3 cut(s) 114, 360, 363
HapII CCGG 2 cut(s) 408, 542
HhaI GCGC 2 cut(s) 359, 388
Hin1II CATG 4 cut(s) 100, 130, 323, 337
Hin6I GCGC 2 cut(s) 357, 386
HinP1I GCGC 2 cut(s) 357, 386
HindIII AAGCTT 1 cut(s) 56
HinfI GANTC 3 cut(s) 415, 469, 538
HpaII CCGG 2 cut(s) 408, 542
HphI GGTGA 1 cut(s) 290
Hpy166II GTNNAC 1 cut(s) 147
Hpy188I TCNGA 1 cut(s) 420
Hpy188III TCNNGA 1 cut(s) 531
Hpy8I GTNNAC 1 cut(s) 147
HpyAV CCTTC 1 cut(s) 208
HpyCH4III ACNGT 3 cut(s) 91, 144, 511
HpyCH4V TGCA 3 cut(s) 116, 218, 365
HpyF10VI GCNNNNNNNGC 3 cut(s) 173, 356, 368
HpyF3I CTNAG 1 cut(s) 517
Hsp92II CATG 4 cut(s) 100, 130, 323, 337
HspAI GCGC 2 cut(s) 357, 386
Ksp22I TGATCA 1 cut(s) 427
Kzo9I GATC 2 cut(s) 123, 427
LmnI GCTCC 1 cut(s) 261
LpnPI CCDG 4 cut(s) 54, 250, 336, 421
Lsp1109I GCAGC 2 cut(s) 100, 349
LweI GCATC 3 cut(s) 163, 344, 492
MaeI CTAG 2 cut(s) 177, 531
MaeIII GTNAC 2 cut(s) 130, 446
MalI GATC 2 cut(s) 125, 429
MboI GATC 2 cut(s) 123, 427
MboII GAAGA 1 cut(s) 298
MhlI GDGCHC 1 cut(s) 73
MluCI AATT 2 cut(s) 75, 492
MlyI GAGTC 1 cut(s) 409
MmeI TCCRAC 1 cut(s) 392
MnlI CCTC 3 cut(s) 78, 326, 497
MspI CCGG 2 cut(s) 408, 542
MvnI CGCG 2 cut(s) 359, 388
MwoI GCNNNNNNNGC 3 cut(s) 173, 356, 368
NcoI CCATGG 1 cut(s) 96
NdeII GATC 2 cut(s) 123, 427
NlaIII CATG 4 cut(s) 100, 130, 323, 337
NlaIV GGNNCC 1 cut(s) 70
NmuCI GTSAC 2 cut(s) 130, 446
PfeI GAWTC 2 cut(s) 469, 538
PinAI ACCGGT 1 cut(s) 407
PkrI GCNGC 3 cut(s) 115, 361, 364
PleI GAGTC 1 cut(s) 409
PpsI GAGTC 1 cut(s) 409
PspN4I GGNNCC 1 cut(s) 70
PstNI CAGNNNCTG 1 cut(s) 237
SatI GCNGC 3 cut(s) 114, 360, 363
Sau3AI GATC 2 cut(s) 123, 427
SchI GAGTC 1 cut(s) 409
SduI GDGCHC 1 cut(s) 73
SetI ASST 5 cut(s) 43, 60, 115, 188, 518
SfaNI GCATC 3 cut(s) 163, 344, 492
SmlI CTYRAG 1 cut(s) 82
SmoI CTYRAG 1 cut(s) 82
Sse9I AATT 2 cut(s) 75, 492
SsiI CCGC 2 cut(s) 359, 388
SspMI CTAG 2 cut(s) 177, 531
StyI CCWWGG 3 cut(s) 18, 96, 280
TaaI ACNGT 3 cut(s) 91, 144, 511
TasI AATT 2 cut(s) 75, 492
TauI GCSGC 1 cut(s) 362
TfiI GAWTC 2 cut(s) 469, 538
TscAI CASTG 1 cut(s) 238
TseFI GTSAC 2 cut(s) 130, 446
TseI GCWGC 2 cut(s) 113, 362
Tsp45I GTSAC 2 cut(s) 130, 446
TspDTI ATGAA 1 cut(s) 319
TspRI CASTG 1 cut(s) 238
XapI RAATTY 1 cut(s) 75
XbaI TCTAGA 1 cut(s) 530
XspI CTAG 2 cut(s) 177, 531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.