MD06G1133900.v1.1

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0003826
Physical Location & Seq
Reverse (-)
27817598 .. 27819015
1418 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1133900.v1.1.491

Sequence Viewer

Length: 546 bp
ATGGCCAGCACAGACTCCAAGTTCCAGCAAAACCCACCTCTGAAAACCCAAAAAATTTTTCTTGGAGTCCAAATCTTCATGAGAACTGTCACAATGACTGCTACATTGGCTGCAACTTGGGTGATGGTCACCAACAAGCAAAGCAGTCAGATTATTGGCATAGAATTTGATGCTCGATATTATTATTCACCTACCTTCAAGTTCTTTGCTTATGCAAATGCTGTTGTGAGTGCCTTCTGCTTGCTCTCCTTGTTCCTCCTTTTCCTTATCCATCGCCATGGTTCGAATCCTACCAACTACTTCATCTTGTTCCTACACGATTTGGTAATGCTGTGTTTGCTGATGGCTGGATGCTCAGCTGCAACTGCAATAGGGTACGTGGGGCAATATGGGAATAGCCACTCGGGCTGGGCGCCAATTTGTGATCACTTTGGGAAGTTCTGCAAAAGAGGGACTGATTCTGTGGTTCTCTCTTACTTGTCTCTCGTTTTCTTGCTCATGCTCACCATCAGCTCTGCAACCAAGTCGAGAAAGATTCAGGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0048037 GO:0051536 GO:0051540
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

182

Amino Acids

20.2

Weight (kDa)

9.4

Isoelectric Point (pI)

28.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 23 - 164 1.6e-38 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 412
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 54, 164
AcyI GRCGYC 1 cut(s) 413
AfaI GTAC 1 cut(s) 377
AgsI TTSAA 1 cut(s) 199
AjuI GAANNNNNNNTTGG 2 cut(s) 42, 74
AluBI AGCT 2 cut(s) 359, 513
AluI AGCT 2 cut(s) 359, 513
Alw26I GTCTC 1 cut(s) 486
Ama87I CYCGRG 1 cut(s) 403
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 2 cut(s) 110, 359
ApoI RAATTY 2 cut(s) 54, 164
AspLEI GCGC 1 cut(s) 415
AsuHPI GGTGA 4 cut(s) 121, 133, 180, 496
AsuII TTCGAA 1 cut(s) 284
AvaI CYCGRG 1 cut(s) 403
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 412
BbvI GCAGC 2 cut(s) 97, 346
BccI CCATC 4 cut(s) 118, 279, 337, 515
BcgI CGANNNNNNTGC 2 cut(s) 507, 541
BclI TGATCA 1 cut(s) 424
BcoDI GTCTC 1 cut(s) 486
BfoI RGCGCY 1 cut(s) 416
BglI GCCNNNNNGGC 1 cut(s) 405
BisI GCNGC 2 cut(s) 111, 360
BlpI GCTNAGC 1 cut(s) 355
BlsI GCNGC 2 cut(s) 112, 361
BmeT110I CYCGRG 1 cut(s) 403
BmiI GGNNCC 1 cut(s) 414
BmsI GCATC 2 cut(s) 160, 341
Bpu1102I GCTNAGC 1 cut(s) 355
Bpu14I TTCGAA 1 cut(s) 284
BsaAI YACGTR 1 cut(s) 379
BsaHI GRCGYC 1 cut(s) 413
BsaJI CCNNGG 1 cut(s) 277
BseDI CCNNGG 1 cut(s) 277
BseGI GGATG 1 cut(s) 356
BseMII CTCAG 1 cut(s) 369
BseXI GCAGC 2 cut(s) 97, 346
BseYI CCCAGC 1 cut(s) 408
BshFI GGCC 1 cut(s) 5
BshNI GGYRCC 1 cut(s) 412
BsiHKCI CYCGRG 1 cut(s) 403
BslFI GGGAC 1 cut(s) 466
BsmAI GTCTC 1 cut(s) 486
BsmFI GGGAC 1 cut(s) 466
BsnI GGCC 1 cut(s) 5
BsoBI CYCGRG 1 cut(s) 403
Bsp119I TTCGAA 1 cut(s) 284
Bsp143I GATC 1 cut(s) 424
Bsp1720I GCTNAGC 1 cut(s) 355
Bsp19I CCATGG 1 cut(s) 277
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 368
BspHI TCATGA 1 cut(s) 78
BspLI GGNNCC 1 cut(s) 414
BspT104I TTCGAA 1 cut(s) 284
BspT107I GGYRCC 1 cut(s) 412
BssECI CCNNGG 1 cut(s) 277
BssMI GATC 1 cut(s) 424
BssNI GRCGYC 1 cut(s) 413
BssT1I CCWWGG 1 cut(s) 277
Bst4CI ACNGT 1 cut(s) 88
BstACI GRCGYC 1 cut(s) 413
BstBAI YACGTR 1 cut(s) 379
BstBI TTCGAA 1 cut(s) 284
BstC8I GCNNGC 2 cut(s) 7, 242
BstDEI CTNAG 1 cut(s) 355
BstDSI CCRYGG 1 cut(s) 277
BstEII GGTNACC 1 cut(s) 127
BstF5I GGATG 1 cut(s) 356
BstH2I RGCGCY 1 cut(s) 416
BstHHI GCGC 1 cut(s) 415
BstKTI GATC 1 cut(s) 427
BstMAI GTCTC 1 cut(s) 486
BstMBI GATC 1 cut(s) 424
BstMWI GCNNNNNNNGC 4 cut(s) 107, 337, 365, 405
BstPI GGTNACC 1 cut(s) 127
BstV1I GCAGC 2 cut(s) 97, 346
BstXI CCANNNNNNTGG 1 cut(s) 278
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 277
BtgZI GCGATG 1 cut(s) 257
BtsCI GGATG 1 cut(s) 356
Cac8I GCNNGC 2 cut(s) 7, 242
CciI TCATGA 1 cut(s) 78
CfoI GCGC 1 cut(s) 415
Csp6I GTAC 1 cut(s) 376
CviAII CATG 3 cut(s) 79, 278, 499
CviJI RGCY 7 cut(s) 5, 110, 347, 359, 399, 408, 513
CviKI_1 RGCY 7 cut(s) 5, 110, 347, 359, 399, 408, 513
CviQI GTAC 1 cut(s) 376
DdeI CTNAG 1 cut(s) 355
DinI GGCGCC 1 cut(s) 414
DpnI GATC 1 cut(s) 426
DpnII GATC 1 cut(s) 424
EaeI YGGCCR 1 cut(s) 3
Eco130I CCWWGG 1 cut(s) 277
Eco88I CYCGRG 1 cut(s) 403
Eco91I GGTNACC 1 cut(s) 127
EcoO65I GGTNACC 1 cut(s) 127
EcoT14I CCWWGG 1 cut(s) 277
EgeI GGCGCC 1 cut(s) 414
EheI GGCGCC 1 cut(s) 414
ErhI CCWWGG 1 cut(s) 277
FaeI CATG 3 cut(s) 82, 281, 502
FaiI YATR 6 cut(s) 80, 161, 213, 279, 390, 500
FaqI GGGAC 1 cut(s) 466
FatI CATG 3 cut(s) 78, 277, 498
FbaI TGATCA 1 cut(s) 424
Fnu4HI GCNGC 2 cut(s) 111, 360
FokI GGATG 1 cut(s) 363
Fsp4HI GCNGC 2 cut(s) 111, 360
GlaI GCGC 1 cut(s) 414
GluI GCNGC 2 cut(s) 111, 360
GsaI CCCAGC 1 cut(s) 412
HaeII RGCGCY 1 cut(s) 416
HaeIII GGCC 1 cut(s) 5
HhaI GCGC 1 cut(s) 415
Hin1I GRCGYC 1 cut(s) 413
Hin1II CATG 3 cut(s) 82, 281, 502
Hin6I GCGC 1 cut(s) 413
HinP1I GCGC 1 cut(s) 413
HinfI GANTC 5 cut(s) 14, 66, 286, 458, 535
HphI GGTGA 4 cut(s) 121, 133, 180, 496
Hpy188I TCNGA 2 cut(s) 42, 150
Hpy188III TCNNGA 2 cut(s) 79, 528
HpyAV CCTTC 2 cut(s) 205, 244
HpyCH4III ACNGT 1 cut(s) 88
HpyCH4IV ACGT 1 cut(s) 378
HpyCH4V TGCA 6 cut(s) 113, 215, 362, 368, 444, 518
HpyF10VI GCNNNNNNNGC 4 cut(s) 107, 337, 365, 405
HpyF3I CTNAG 1 cut(s) 355
HpySE526I ACGT 1 cut(s) 378
Hsp92I GRCGYC 1 cut(s) 413
Hsp92II CATG 3 cut(s) 82, 281, 502
HspAI GCGC 1 cut(s) 413
KasI GGCGCC 1 cut(s) 412
Ksp22I TGATCA 1 cut(s) 424
Kzo9I GATC 1 cut(s) 424
LpnPI CCDG 5 cut(s) 19, 38, 333, 394, 524
Lsp1109I GCAGC 2 cut(s) 97, 346
LweI GCATC 2 cut(s) 160, 341
MaeII ACGT 1 cut(s) 378
MaeIII GTNAC 2 cut(s) 88, 127
MalI GATC 1 cut(s) 426
MboI GATC 1 cut(s) 424
MboII GAAGA 1 cut(s) 67
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 54, 164, 417
MluNI TGGCCA 1 cut(s) 5
Mly113I GGCGCC 1 cut(s) 413
MlyI GAGTC 2 cut(s) 8, 75
MnlI CCTC 3 cut(s) 48, 266, 443
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MslI CAYNNNNRTG 1 cut(s) 276
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 359
MwoI GCNNNNNNNGC 4 cut(s) 107, 337, 365, 405
NarI GGCGCC 1 cut(s) 413
NcoI CCATGG 1 cut(s) 277
NdeII GATC 1 cut(s) 424
NlaIII CATG 3 cut(s) 82, 281, 502
NlaIV GGNNCC 1 cut(s) 414
NmuCI GTSAC 2 cut(s) 88, 127
NspV TTCGAA 1 cut(s) 284
PagI TCATGA 1 cut(s) 78
PfeI GAWTC 3 cut(s) 286, 458, 535
PkrI GCNGC 2 cut(s) 112, 361
PleI GAGTC 2 cut(s) 8, 74
PluTI GGCGCC 1 cut(s) 416
PpsI GAGTC 2 cut(s) 8, 74
Ppu21I YACGTR 1 cut(s) 379
PspEI GGTNACC 1 cut(s) 127
PspFI CCCAGC 1 cut(s) 408
PspN4I GGNNCC 1 cut(s) 414
PvuII CAGCTG 1 cut(s) 359
RsaI GTAC 1 cut(s) 377
RsaNI GTAC 1 cut(s) 376
RseI CAYNNNNRTG 1 cut(s) 276
SatI GCNGC 2 cut(s) 111, 360
Sau3AI GATC 1 cut(s) 424
SchI GAGTC 2 cut(s) 8, 75
SetI ASST 7 cut(s) 40, 193, 197, 361, 381, 515, 543
SfaNI GCATC 2 cut(s) 160, 341
SfoI GGCGCC 1 cut(s) 414
SfuI TTCGAA 1 cut(s) 284
SmiMI CAYNNNNRTG 1 cut(s) 276
Sse9I AATT 3 cut(s) 54, 164, 417
SspDI GGCGCC 1 cut(s) 412
StyI CCWWGG 1 cut(s) 277
TaaI ACNGT 1 cut(s) 88
TaiI ACGT 1 cut(s) 381
TaqI TCGA 3 cut(s) 175, 284, 527
TasI AATT 3 cut(s) 54, 164, 417
TfiI GAWTC 3 cut(s) 286, 458, 535
TseFI GTSAC 2 cut(s) 88, 127
TseI GCWGC 2 cut(s) 110, 359
Tsp45I GTSAC 2 cut(s) 88, 127
TspDTI ATGAA 2 cut(s) 67, 292
XapI RAATTY 2 cut(s) 54, 164
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.