Rh2AG670800

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0003826
Physical Location & Seq
Forward (+)
88446533 .. 88448285
1753 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG670800.1

Sequence Viewer

Length: 594 bp
ATGGCTTCAGAGTCTTCGACGGTGGCTAAAGACGAGAAGAATATTGTGTCCTCTTTTGTTGATTCGAGTAGTCAACGGCGGAAAACGAGTCTCTTGATGGCTCAAGCGGGTCTCAGAATCTTAGTTGTTGCCTTCACATTGGTCGCAATCTCTGTAATGGTCACCAACCGCCAGTCCGTCGTCATCTTTGGATTGAACTTCCAAGCACGATACTCCTACTCGTCTGCCTTGACGTTCTTGGTTGCTGCGGATTCTGTGTTGTGTTCCTTCTCAGCACTGTCGTTGGTCTTCATGTACATCTTAAGCCGTTCTGGGACAACTTCGCCTCTCAAGAAGTACTTCTTCCTCTTCTTGCTTGATACGGTGATGATGGTGTTAATAATAGCTGGATGTGCAGCTGCGACTGCAATAGGGTACTTGGGAAAGTATGGGGAAGAACACATGACTTGGCATGCAACTTGTGGATATGTGTCCAAGTTCTGCAACAGAATGTCCATCTCTCTTGCATTTTCATACTTGGCCTTCTTTGCCTGCTTGCTTCTCAATCTCATGTCGGCTCATGCTCTCATCTATCGCCCGATTATCAAGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0048037 GO:0051536 GO:0051540
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

197

Amino Acids

21.65

Weight (kDa)

9.4

Isoelectric Point (pI)

40.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 30 - 177 9.1e-37 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 79, 107, 169, 248
AfaI GTAC 3 cut(s) 296, 338, 416
AflII CTTAAG 1 cut(s) 301
AgsI TTSAA 1 cut(s) 196
AluBI AGCT 2 cut(s) 386, 398
AluI AGCT 2 cut(s) 386, 398
Alw26I GTCTC 2 cut(s) 95, 116
AoxI GGCC 1 cut(s) 519
ApeKI GCWGC 3 cut(s) 245, 395, 398
Asp700I GAANNNNTTC 1 cut(s) 338
AsuHPI GGTGA 2 cut(s) 154, 376
BbsI GAAGAC 2 cut(s) 6, 280
BbvI GCAGC 3 cut(s) 232, 385, 407
BccI CCATC 3 cut(s) 91, 364, 503
BceAI ACGGC 2 cut(s) 92, 291
BcoDI GTCTC 2 cut(s) 95, 116
BfrI CTTAAG 1 cut(s) 301
BisI GCNGC 3 cut(s) 246, 396, 399
BlsI GCNGC 3 cut(s) 247, 397, 400
BmcAI AGTACT 1 cut(s) 338
BpiI GAAGAC 2 cut(s) 6, 280
BpuEI CTTGAG 2 cut(s) 87, 314
BsaI GGTCTC 1 cut(s) 116
Bse1I ACTGG 1 cut(s) 172
BseGI GGATG 1 cut(s) 395
BseMII CTCAG 2 cut(s) 127, 285
BseNI ACTGG 1 cut(s) 172
BseXI GCAGC 3 cut(s) 232, 385, 407
BsgI GTGCAG 1 cut(s) 414
BshFI GGCC 1 cut(s) 521
BslFI GGGAC 1 cut(s) 328
BsmAI GTCTC 2 cut(s) 95, 116
BsmFI GGGAC 1 cut(s) 328
BsnI GGCC 1 cut(s) 521
Bso31I GGTCTC 1 cut(s) 116
Bsp1407I TGTACA 1 cut(s) 294
BspACI CCGC 4 cut(s) 79, 107, 169, 248
BspANI GGCC 1 cut(s) 521
BspCNI CTCAG 2 cut(s) 126, 284
BspTI CTTAAG 1 cut(s) 301
BspTNI GGTCTC 1 cut(s) 116
BsrGI TGTACA 1 cut(s) 294
BsrI ACTGG 1 cut(s) 172
Bst4CI ACNGT 3 cut(s) 22, 279, 364
Bst6I CTCTTC 1 cut(s) 353
BstAFI CTTAAG 1 cut(s) 301
BstAUI TGTACA 1 cut(s) 294
BstC8I GCNNGC 3 cut(s) 453, 532, 536
BstDEI CTNAG 3 cut(s) 113, 121, 271
BstEII GGTNACC 1 cut(s) 160
BstF5I GGATG 1 cut(s) 395
BstMAI GTCTC 2 cut(s) 95, 116
BstMWI GCNNNNNNNGC 3 cut(s) 392, 404, 527
BstNSI RCATGY 1 cut(s) 455
BstPI GGTNACC 1 cut(s) 160
BstV1I GCAGC 3 cut(s) 232, 385, 407
BstV2I GAAGAC 2 cut(s) 6, 280
BsuRI GGCC 1 cut(s) 521
BtsCI GGATG 1 cut(s) 395
BtsIMutI CAGTG 1 cut(s) 275
Cac8I GCNNGC 3 cut(s) 453, 532, 536
Csp6I GTAC 3 cut(s) 295, 337, 415
CviAII CATG 5 cut(s) 292, 442, 452, 550, 560
CviJI RGCY 8 cut(s) 5, 26, 101, 306, 386, 398, 521, 557
CviKI_1 RGCY 8 cut(s) 5, 26, 101, 306, 386, 398, 521, 557
CviQI GTAC 3 cut(s) 295, 337, 415
DdeI CTNAG 3 cut(s) 113, 121, 271
Eam1104I CTCTTC 1 cut(s) 353
EarI CTCTTC 1 cut(s) 353
EciI GGCGGA 1 cut(s) 94
Eco31I GGTCTC 1 cut(s) 116
Eco91I GGTNACC 1 cut(s) 160
EcoO65I GGTNACC 1 cut(s) 160
FaeI CATG 5 cut(s) 295, 445, 455, 553, 563
FaiI YATR 8 cut(s) 293, 429, 443, 453, 468, 514, 551, 561
FalI AAGNNNNNCTT 4 cut(s) 323, 355, 326, 358
FaqI GGGAC 1 cut(s) 328
FatI CATG 5 cut(s) 291, 441, 451, 549, 559
FauI CCCGC 1 cut(s) 100
Fnu4HI GCNGC 3 cut(s) 246, 396, 399
FokI GGATG 1 cut(s) 402
Fsp4HI GCNGC 3 cut(s) 246, 396, 399
GluI GCNGC 3 cut(s) 246, 396, 399
HaeIII GGCC 1 cut(s) 521
Hin1II CATG 5 cut(s) 295, 445, 455, 553, 563
HincII GTYRAC 1 cut(s) 74
HindII GTYRAC 1 cut(s) 74
HinfI GANTC 5 cut(s) 11, 62, 88, 117, 251
HphI GGTGA 2 cut(s) 154, 376
Hpy166II GTNNAC 1 cut(s) 74
Hpy188I TCNGA 2 cut(s) 10, 116
Hpy188III TCNNGA 3 cut(s) 94, 331, 586
Hpy8I GTNNAC 1 cut(s) 74
Hpy99I CGWCG 2 cut(s) 22, 182
HpyAV CCTTC 3 cut(s) 142, 277, 532
HpyCH4III ACNGT 3 cut(s) 22, 279, 364
HpyCH4IV ACGT 1 cut(s) 233
HpyCH4V TGCA 5 cut(s) 395, 407, 455, 483, 506
HpyF10VI GCNNNNNNNGC 3 cut(s) 392, 404, 527
HpyF3I CTNAG 3 cut(s) 113, 121, 271
HpySE526I ACGT 1 cut(s) 233
Hsp92II CATG 5 cut(s) 295, 445, 455, 553, 563
LpnPI CCDG 4 cut(s) 185, 297, 372, 544
Lsp1109I GCAGC 3 cut(s) 232, 385, 407
MaeII ACGT 1 cut(s) 233
MaeIII GTNAC 1 cut(s) 160
MboII GAAGA 6 cut(s) 6, 49, 280, 334, 340, 446
MlyI GAGTC 2 cut(s) 20, 97
MnlI CCTC 3 cut(s) 61, 336, 356
MroXI GAANNNNTTC 1 cut(s) 338
MseI TTAA 2 cut(s) 302, 377
MspA1I CMGCKG 1 cut(s) 398
MspCI CTTAAG 1 cut(s) 301
MwoI GCNNNNNNNGC 3 cut(s) 392, 404, 527
NlaIII CATG 5 cut(s) 295, 445, 455, 553, 563
NmuCI GTSAC 1 cut(s) 160
NspI RCATGY 1 cut(s) 455
PaeI GCATGC 1 cut(s) 455
PdmI GAANNNNTTC 1 cut(s) 338
PfeI GAWTC 3 cut(s) 62, 117, 251
PkrI GCNGC 3 cut(s) 247, 397, 400
PleI GAGTC 2 cut(s) 19, 96
PpsI GAGTC 2 cut(s) 19, 96
PspEI GGTNACC 1 cut(s) 160
PvuII CAGCTG 1 cut(s) 398
RsaI GTAC 3 cut(s) 296, 338, 416
RsaNI GTAC 3 cut(s) 295, 337, 415
SaqAI TTAA 2 cut(s) 302, 377
SatI GCNGC 3 cut(s) 246, 396, 399
ScaI AGTACT 1 cut(s) 338
SchI GAGTC 2 cut(s) 20, 97
SetI ASST 3 cut(s) 236, 388, 400
SmlI CTYRAG 3 cut(s) 102, 301, 329
SmoI CTYRAG 3 cut(s) 102, 301, 329
SphI GCATGC 1 cut(s) 455
SsiI CCGC 4 cut(s) 79, 107, 169, 248
SspI AATATT 1 cut(s) 43
TaaI ACNGT 3 cut(s) 22, 279, 364
TaiI ACGT 1 cut(s) 236
TaqI TCGA 2 cut(s) 17, 65
TatI WGTACW 2 cut(s) 294, 336
TfiI GAWTC 3 cut(s) 62, 117, 251
Tru1I TTAA 2 cut(s) 302, 377
Tru9I TTAA 2 cut(s) 302, 377
TscAI CASTG 1 cut(s) 282
TseFI GTSAC 1 cut(s) 160
TseI GCWGC 3 cut(s) 245, 395, 398
Tsp45I GTSAC 1 cut(s) 160
TspDTI ATGAA 2 cut(s) 280, 501
TspGWI ACGGA 1 cut(s) 166
TspRI CASTG 1 cut(s) 282
Vha464I CTTAAG 1 cut(s) 301
XceI RCATGY 1 cut(s) 455
XmnI GAANNNNTTC 1 cut(s) 338
ZrmI AGTACT 1 cut(s) 338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.