MD14G1150200.v1.1

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0003826
Physical Location & Seq
Reverse (-)
24350230 .. 24351960
1731 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1150200.v1.1.491

Sequence Viewer

Length: 540 bp
ATGACAAGCACAGAATCCAAGTTCCAGCAAAACCCACCTCTGAAAACCCAAAAGATTTTTCTTGGAGTCCAAATCTTCATGAGGACTGTCACAGTGGCTGCTACATTGGCTGCAACTTGGTTGATGGTCACCAACAAGCAGACCAGTCAGATTATTGGCATAGAATTTGATGCTCGTTATTATTATTCACCTACCTTCAAGTTCTTTGCTTATGCAAATGCTGTTGTGAGTGCCTTCTGCTTGCTCTCCTTGTTCCTCGTTTTTCTTATCCACCGCAATGGCTCGAATCCCGCCAACTACTTCATCTTGTTCCTGCACGATTTGGTGATGCTGTGTTTGCTGATGGCTGGATGCTCGGCTGCAACTGCAATAGGGTACGTGGGGCAATATGGGAATAGCCACTCGGGCTGGGCACCAATTTGTGATCACTTTGGGAAGTTCTGCAAAAGAGGGACCGATTCTGTGGTTCTCTCCTACTTGTCTGTCATTTTCTTGCTCACGCTCACCATCAGCTCTGCAACCAAGTCTGGAAAGATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0048037 GO:0051536 GO:0051540
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

180

Amino Acids

19.79

Weight (kDa)

9.28

Isoelectric Point (pI)

27.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 23 - 164 2.3e-39 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 412
AciI CCGC 2 cut(s) 274, 291
AcsI RAATTY 1 cut(s) 164
AfaI GTAC 1 cut(s) 377
AgsI TTSAA 1 cut(s) 199
AjuI GAANNNNNNNTTGG 2 cut(s) 42, 74
AluBI AGCT 1 cut(s) 513
AluI AGCT 1 cut(s) 513
AlwNI CAGNNNCTG 1 cut(s) 98
Ama87I CYCGRG 1 cut(s) 403
ApeKI GCWGC 3 cut(s) 98, 110, 359
ApoI RAATTY 1 cut(s) 164
AspS9I GGNCC 1 cut(s) 453
AsuHPI GGTGA 4 cut(s) 121, 180, 337, 496
AvaI CYCGRG 1 cut(s) 403
AvaII GGWCC 1 cut(s) 453
BaeGI GKGCMC 1 cut(s) 415
BanI GGYRCC 1 cut(s) 412
BbvI GCAGC 3 cut(s) 85, 97, 346
BccI CCATC 3 cut(s) 118, 337, 515
BclI TGATCA 1 cut(s) 424
BglI GCCNNNNNGGC 1 cut(s) 405
BisI GCNGC 3 cut(s) 99, 111, 360
BlsI GCNGC 3 cut(s) 100, 112, 361
Bme18I GGWCC 1 cut(s) 453
BmeT110I CYCGRG 1 cut(s) 403
BmgT120I GGNCC 1 cut(s) 453
BmiI GGNNCC 2 cut(s) 414, 454
BmsI GCATC 3 cut(s) 160, 318, 341
BsaAI YACGTR 1 cut(s) 379
Bse1I ACTGG 1 cut(s) 144
Bse3DI GCAATG 1 cut(s) 283
BseGI GGATG 1 cut(s) 356
BseMI GCAATG 1 cut(s) 283
BseNI ACTGG 1 cut(s) 144
BseSI GKGCMC 1 cut(s) 415
BseXI GCAGC 3 cut(s) 85, 97, 346
BseYI CCCAGC 1 cut(s) 408
BsgI GTGCAG 1 cut(s) 299
BshNI GGYRCC 1 cut(s) 412
BsiHKCI CYCGRG 1 cut(s) 403
BslFI GGGAC 1 cut(s) 466
BsmFI GGGAC 1 cut(s) 466
BsoBI CYCGRG 1 cut(s) 403
Bsp1286I GDGCHC 1 cut(s) 415
Bsp143I GATC 1 cut(s) 424
BspACI CCGC 2 cut(s) 274, 291
BspHI TCATGA 1 cut(s) 78
BspLI GGNNCC 2 cut(s) 414, 454
BspT107I GGYRCC 1 cut(s) 412
BsrDI GCAATG 1 cut(s) 283
BsrI ACTGG 1 cut(s) 144
BssMI GATC 1 cut(s) 424
Bst4CI ACNGT 2 cut(s) 88, 94
BstBAI YACGTR 1 cut(s) 379
BstC8I GCNNGC 1 cut(s) 242
BstEII GGTNACC 1 cut(s) 127
BstF5I GGATG 1 cut(s) 356
BstKTI GATC 1 cut(s) 427
BstMBI GATC 1 cut(s) 424
BstMWI GCNNNNNNNGC 4 cut(s) 107, 337, 365, 405
BstPI GGTNACC 1 cut(s) 127
BstSLI GKGCMC 1 cut(s) 415
BstV1I GCAGC 3 cut(s) 85, 97, 346
BstXI CCANNNNNNTGG 1 cut(s) 278
BtsCI GGATG 1 cut(s) 356
BtsIMutI CAGTG 1 cut(s) 99
Cac8I GCNNGC 1 cut(s) 242
CaiI CAGNNNCTG 1 cut(s) 98
CciI TCATGA 1 cut(s) 78
Cfr13I GGNCC 1 cut(s) 453
Csp6I GTAC 1 cut(s) 376
CviAII CATG 1 cut(s) 79
CviJI RGCY 8 cut(s) 98, 110, 282, 347, 359, 399, 408, 513
CviKI_1 RGCY 8 cut(s) 98, 110, 282, 347, 359, 399, 408, 513
CviQI GTAC 1 cut(s) 376
DpnI GATC 1 cut(s) 426
DpnII GATC 1 cut(s) 424
Eco47I GGWCC 1 cut(s) 453
Eco88I CYCGRG 1 cut(s) 403
Eco91I GGTNACC 1 cut(s) 127
EcoO65I GGTNACC 1 cut(s) 127
FaeI CATG 1 cut(s) 82
FaiI YATR 4 cut(s) 80, 161, 213, 390
FaqI GGGAC 1 cut(s) 466
FatI CATG 1 cut(s) 78
FauI CCCGC 1 cut(s) 298
FbaI TGATCA 1 cut(s) 424
Fnu4HI GCNGC 3 cut(s) 99, 111, 360
FokI GGATG 1 cut(s) 363
Fsp4HI GCNGC 3 cut(s) 99, 111, 360
GluI GCNGC 3 cut(s) 99, 111, 360
GsaI CCCAGC 1 cut(s) 412
Hin1II CATG 1 cut(s) 82
HinfI GANTC 4 cut(s) 14, 66, 286, 458
HphI GGTGA 4 cut(s) 121, 180, 337, 496
Hpy188I TCNGA 2 cut(s) 42, 150
Hpy188III TCNNGA 2 cut(s) 79, 528
HpyAV CCTTC 2 cut(s) 205, 244
HpyCH4III ACNGT 2 cut(s) 88, 94
HpyCH4IV ACGT 1 cut(s) 378
HpyCH4V TGCA 7 cut(s) 113, 215, 316, 362, 368, 444, 518
HpyF10VI GCNNNNNNNGC 4 cut(s) 107, 337, 365, 405
HpySE526I ACGT 1 cut(s) 378
Hsp92II CATG 1 cut(s) 82
Ksp22I TGATCA 1 cut(s) 424
Kzo9I GATC 1 cut(s) 424
LpnPI CCDG 6 cut(s) 38, 157, 326, 333, 394, 513
Lsp1109I GCAGC 3 cut(s) 85, 97, 346
LweI GCATC 3 cut(s) 160, 318, 341
MaeII ACGT 1 cut(s) 378
MaeIII GTNAC 2 cut(s) 88, 127
MalI GATC 1 cut(s) 426
MboI GATC 1 cut(s) 424
MboII GAAGA 1 cut(s) 67
MhlI GDGCHC 1 cut(s) 415
MluCI AATT 2 cut(s) 164, 417
MlyI GAGTC 1 cut(s) 75
MnlI CCTC 4 cut(s) 48, 75, 266, 443
MslI CAYNNNNRTG 1 cut(s) 276
MwoI GCNNNNNNNGC 4 cut(s) 107, 337, 365, 405
NdeII GATC 1 cut(s) 424
NlaIII CATG 1 cut(s) 82
NlaIV GGNNCC 2 cut(s) 414, 454
NmeAIII GCCGAG 1 cut(s) 335
NmuCI GTSAC 2 cut(s) 88, 127
PagI TCATGA 1 cut(s) 78
PfeI GAWTC 3 cut(s) 14, 286, 458
PkrI GCNGC 3 cut(s) 100, 112, 361
PleI GAGTC 1 cut(s) 74
PpsI GAGTC 1 cut(s) 74
Ppu21I YACGTR 1 cut(s) 379
PspEI GGTNACC 1 cut(s) 127
PspFI CCCAGC 1 cut(s) 408
PspN4I GGNNCC 2 cut(s) 414, 454
PspPI GGNCC 1 cut(s) 453
PstNI CAGNNNCTG 1 cut(s) 98
RsaI GTAC 1 cut(s) 377
RsaNI GTAC 1 cut(s) 376
RseI CAYNNNNRTG 1 cut(s) 276
SatI GCNGC 3 cut(s) 99, 111, 360
Sau3AI GATC 1 cut(s) 424
Sau96I GGNCC 1 cut(s) 453
SchI GAGTC 1 cut(s) 75
SduI GDGCHC 1 cut(s) 415
SetI ASST 5 cut(s) 40, 193, 197, 381, 515
SfaNI GCATC 3 cut(s) 160, 318, 341
SinI GGWCC 1 cut(s) 453
SmiMI CAYNNNNRTG 1 cut(s) 276
Sse9I AATT 2 cut(s) 164, 417
SsiI CCGC 2 cut(s) 274, 291
TaaI ACNGT 2 cut(s) 88, 94
TaiI ACGT 1 cut(s) 381
TaqI TCGA 1 cut(s) 284
TaqII GACCGA 1 cut(s) 470
TasI AATT 2 cut(s) 164, 417
TfiI GAWTC 3 cut(s) 14, 286, 458
TscAI CASTG 1 cut(s) 99
TseFI GTSAC 2 cut(s) 88, 127
TseI GCWGC 3 cut(s) 98, 110, 359
Tsp45I GTSAC 2 cut(s) 88, 127
TspDTI ATGAA 2 cut(s) 67, 292
TspRI CASTG 1 cut(s) 99
VpaK11BI GGWCC 1 cut(s) 453
XapI RAATTY 1 cut(s) 164
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.