AT4G25250

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0005396
Physical Location & Seq
Forward (+)
12934562 .. 12935470
909 bp
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UTR
Exon/CDS
Intron
AT4G25250.1

Sequence Viewer

Length: 600 bp
ATGTTGCGTTTCGTGGTACTCTCTCTAACTCTCATGGTTTTCATCAACTCATCAAACTTTCCAAAGACAGCAGCCACCCCACCAGGCACGTACCAAAACCACACAACGTACGTAAAAACGGCATGCAACTCAACAACATACCCTACAATGTGCTACAACTGTCTGTCCTCCTATTCCTCCACCATCAAGTCCGACCCAATCAAACTATGCACAACATCGCTAAATCTCAACGTCAAATCCGCTAAAAACGCCACATTAGTCGTCTCTAACCTTCTCCAAAAGGCCAAAGCCGCGAAAAGCCATGAGGTCTCGATCCTTAAGGACTGCGTGGACGAGATGAAGGACACTATCGACGAGCTTAAGCAAGCGGTCGCCGAGATGAAGTATGTCAGAGGCGGAGGGAAAACTACGGAGGAGCATTTAAAGAACGTGAAGACGTGGGTGAGCTCGGCTTTGACCGACGAGGGCACGTGTACGGACGGATTCGAGGAAGGGAGAGTAAATGTGGAAACAAAGAAGAAGGTGAAGAAGGCTATTTCTGAGCTTTCAAAGACTACAAGCAATACTTTAGCCCTTCTTACTCATTATTTAAGTTATTGA

Protein Analysis

199

Amino Acids

21.9

Weight (kDa)

9.03

Isoelectric Point (pI)

25.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 37 - 192 2.4e-35 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 293
AciI CCGC 4 cut(s) 240, 291, 368, 396
AclWI GGATC 1 cut(s) 307
AcvI CACGTG 1 cut(s) 471
AfaI GTAC 4 cut(s) 18, 92, 110, 475
AflII CTTAAG 2 cut(s) 317, 359
AflIII ACRYGT 1 cut(s) 470
AgsI TTSAA 1 cut(s) 549
AjiI CACGTC 1 cut(s) 438
AjnI CCWGG 1 cut(s) 82
AluBI AGCT 3 cut(s) 358, 447, 544
AluI AGCT 3 cut(s) 358, 447, 544
Alw21I GWGCWC 1 cut(s) 449
Alw26I GTCTC 2 cut(s) 268, 313
AlwI GGATC 1 cut(s) 307
AoxI GGCC 1 cut(s) 282
ApeKI GCWGC 1 cut(s) 71
AsuHPI GGTGA 2 cut(s) 454, 535
BaeGI GKGCMC 1 cut(s) 470
BanII GRGCYC 1 cut(s) 449
BbrPI CACGTG 1 cut(s) 471
BbsI GAAGAC 1 cut(s) 440
Bbv12I GWGCWC 1 cut(s) 449
BbvI GCAGC 1 cut(s) 83
BccI CCATC 1 cut(s) 191
BceAI ACGGC 1 cut(s) 135
BciT130I CCWGG 1 cut(s) 84
BcoDI GTCTC 2 cut(s) 268, 313
BfrI CTTAAG 2 cut(s) 317, 359
BisI GCNGC 2 cut(s) 72, 291
BlsI GCNGC 2 cut(s) 73, 292
Bme1390I CCNGG 1 cut(s) 84
BmgBI CACGTC 1 cut(s) 438
BmrFI CCNGG 1 cut(s) 84
BpiI GAAGAC 1 cut(s) 440
BsaAI YACGTR 3 cut(s) 90, 112, 471
BsaI GGTCTC 1 cut(s) 313
BseBI CCWGG 1 cut(s) 84
BseMII CTCAG 1 cut(s) 531
BseRI GAGGAG 1 cut(s) 428
BseSI GKGCMC 1 cut(s) 470
BseXI GCAGC 1 cut(s) 83
Bsh1236I CGCG 1 cut(s) 293
Bsh1285I CGRYCG 1 cut(s) 372
BshFI GGCC 1 cut(s) 284
BsiEI CGRYCG 1 cut(s) 372
BsiHKAI GWGCWC 1 cut(s) 449
BsiWI CGTACG 1 cut(s) 108
BsmAI GTCTC 2 cut(s) 268, 313
BsmBI CGTCTC 1 cut(s) 268
BsnI GGCC 1 cut(s) 284
Bso31I GGTCTC 1 cut(s) 313
Bsp1286I GDGCHC 2 cut(s) 449, 470
Bsp143I GATC 1 cut(s) 312
BspACI CCGC 4 cut(s) 240, 291, 368, 396
BspANI GGCC 1 cut(s) 284
BspCNI CTCAG 1 cut(s) 532
BspFNI CGCG 1 cut(s) 293
BspPI GGATC 1 cut(s) 307
BspTI CTTAAG 2 cut(s) 317, 359
BspTNI GGTCTC 1 cut(s) 313
BssMI GATC 1 cut(s) 312
Bst2UI CCWGG 1 cut(s) 84
Bst4CI ACNGT 1 cut(s) 161
BstAFI CTTAAG 2 cut(s) 317, 359
BstBAI YACGTR 3 cut(s) 90, 112, 471
BstC8I GCNNGC 2 cut(s) 124, 366
BstDEI CTNAG 1 cut(s) 540
BstFNI CGCG 1 cut(s) 293
BstKTI GATC 1 cut(s) 315
BstMAI GTCTC 2 cut(s) 268, 313
BstMBI GATC 1 cut(s) 312
BstMCI CGRYCG 1 cut(s) 372
BstMWI GCNNNNNNNGC 2 cut(s) 248, 290
BstNI CCWGG 1 cut(s) 84
BstNSI RCATGY 1 cut(s) 126
BstSCI CCNGG 1 cut(s) 82
BstSLI GKGCMC 1 cut(s) 470
BstSNI TACGTA 1 cut(s) 112
BstUI CGCG 1 cut(s) 293
BstV1I GCAGC 1 cut(s) 83
BstV2I GAAGAC 1 cut(s) 440
BsuRI GGCC 1 cut(s) 284
BtgZI GCGATG 1 cut(s) 201
BtrI CACGTC 1 cut(s) 438
Cac8I GCNNGC 2 cut(s) 124, 366
Csp6I GTAC 4 cut(s) 17, 91, 109, 474
CviAII CATG 3 cut(s) 34, 123, 302
CviQI GTAC 4 cut(s) 17, 91, 109, 474
DdeI CTNAG 1 cut(s) 540
DpnI GATC 1 cut(s) 314
DpnII GATC 1 cut(s) 312
DraI TTTAAA 1 cut(s) 423
EciI GGCGGA 1 cut(s) 411
Ecl136II GAGCTC 1 cut(s) 447
Eco105I TACGTA 1 cut(s) 112
Eco24I GRGCYC 1 cut(s) 449
Eco31I GGTCTC 1 cut(s) 313
Eco53kI GAGCTC 1 cut(s) 447
Eco72I CACGTG 1 cut(s) 471
EcoICRI GAGCTC 1 cut(s) 447
EcoRII CCWGG 1 cut(s) 82
EcoT38I GRGCYC 1 cut(s) 449
Esp3I CGTCTC 1 cut(s) 268
FaeI CATG 3 cut(s) 37, 126, 305
FaiI YATR 6 cut(s) 35, 124, 139, 208, 303, 387
FalI AAGNNNNNCTT 2 cut(s) 550, 582
FatI CATG 3 cut(s) 33, 122, 301
Fnu4HI GCNGC 2 cut(s) 72, 291
FriOI GRGCYC 1 cut(s) 449
Fsp4HI GCNGC 2 cut(s) 72, 291
GluI GCNGC 2 cut(s) 72, 291
HaeIII GGCC 1 cut(s) 284
Hin1II CATG 3 cut(s) 37, 126, 305
HinfI GANTC 1 cut(s) 483
HphI GGTGA 2 cut(s) 454, 535
Hpy166II GTNNAC 2 cut(s) 331, 474
Hpy188I TCNGA 3 cut(s) 193, 392, 541
Hpy188III TCNNGA 1 cut(s) 310
Hpy8I GTNNAC 2 cut(s) 331, 474
Hpy99I CGWCG 2 cut(s) 356, 464
HpyAV CCTTC 6 cut(s) 281, 334, 485, 514, 523, 584
HpyCH4III ACNGT 1 cut(s) 161
HpyCH4IV ACGT 7 cut(s) 89, 107, 111, 231, 429, 437, 470
HpyCH4V TGCA 2 cut(s) 126, 210
HpyF10VI GCNNNNNNNGC 2 cut(s) 248, 290
HpyF3I CTNAG 1 cut(s) 540
HpySE526I ACGT 7 cut(s) 89, 107, 111, 231, 429, 437, 470
Hsp92II CATG 3 cut(s) 37, 126, 305
Kzo9I GATC 1 cut(s) 312
LmnI GCTCC 1 cut(s) 415
LpnPI CCDG 2 cut(s) 69, 96
Lsp1109I GCAGC 1 cut(s) 83
MaeII ACGT 7 cut(s) 89, 107, 111, 231, 429, 437, 470
MalI GATC 1 cut(s) 314
MboI GATC 1 cut(s) 312
MboII GAAGA 3 cut(s) 445, 529, 538
MhlI GDGCHC 2 cut(s) 449, 470
MmeI TCCRAC 1 cut(s) 216
MnlI CCTC 8 cut(s) 178, 187, 298, 386, 392, 406, 457, 481
MseI TTAA 4 cut(s) 318, 360, 422, 590
MspCI CTTAAG 2 cut(s) 317, 359
MspR9I CCNGG 1 cut(s) 84
MvaI CCWGG 1 cut(s) 84
MvnI CGCG 1 cut(s) 293
MwoI GCNNNNNNNGC 2 cut(s) 248, 290
NdeII GATC 1 cut(s) 312
NlaIII CATG 3 cut(s) 37, 126, 305
NmeAIII GCCGAG 2 cut(s) 400, 428
NspI RCATGY 1 cut(s) 126
PaeI GCATGC 1 cut(s) 126
PfeI GAWTC 1 cut(s) 483
Pfl23II CGTACG 1 cut(s) 108
PkrI GCNGC 2 cut(s) 73, 292
PmaCI CACGTG 1 cut(s) 471
PmlI CACGTG 1 cut(s) 471
Ppu21I YACGTR 3 cut(s) 90, 112, 471
Psp124BI GAGCTC 1 cut(s) 449
Psp6I CCWGG 1 cut(s) 82
PspCI CACGTG 1 cut(s) 471
PspGI CCWGG 1 cut(s) 82
PspLI CGTACG 1 cut(s) 108
RsaI GTAC 4 cut(s) 18, 92, 110, 475
RsaNI GTAC 4 cut(s) 17, 91, 109, 474
SacI GAGCTC 1 cut(s) 449
SaqAI TTAA 4 cut(s) 318, 360, 422, 590
SatI GCNGC 2 cut(s) 72, 291
Sau3AI GATC 1 cut(s) 312
ScrFI CCNGG 1 cut(s) 84
SduI GDGCHC 2 cut(s) 449, 470
SmlI CTYRAG 2 cut(s) 317, 359
SmoI CTYRAG 2 cut(s) 317, 359
SnaBI TACGTA 1 cut(s) 112
SphI GCATGC 1 cut(s) 126
SsiI CCGC 4 cut(s) 240, 291, 368, 396
SstI GAGCTC 1 cut(s) 449
StyD4I CCNGG 1 cut(s) 82
TaaI ACNGT 1 cut(s) 161
TaiI ACGT 7 cut(s) 92, 110, 114, 234, 432, 440, 473
TaqI TCGA 3 cut(s) 311, 351, 486
TaqII GACCGA 1 cut(s) 473
TauI GCSGC 1 cut(s) 293
TfiI GAWTC 1 cut(s) 483
Tru1I TTAA 4 cut(s) 318, 360, 422, 590
Tru9I TTAA 4 cut(s) 318, 360, 422, 590
TseI GCWGC 1 cut(s) 71
TspDTI ATGAA 3 cut(s) 31, 353, 395
TspGWI ACGGA 3 cut(s) 425, 491, 495
Vha464I CTTAAG 2 cut(s) 317, 359
XceI RCATGY 1 cut(s) 126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.