MD00G1105100.v1.1

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0005396
Physical Location & Seq
Reverse (-)
21993386 .. 21994012
627 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1105100.v1.1.491

Sequence Viewer

Length: 627 bp
ATGGGTGCTCAAACTTTCACAACCCCAACTTCAAATTCACACCATGTTCTCACATTTCTTCTCCCAATTATTCTACTCATCATCATGACAAACATGCAAACAACCGTGGCAACCTCCTCTTCCTCTCAAACCTACAAAACCTACGTCAAAACCGCCTGCAATTCAACCACATACCCACTCGTTTGCTACCAATCCCTTTCCTCGTACGCTTCCAAAGTCAAATCTAACCCTCGAAAGCTTTGCATCTATGCCCTCTCCGTGACCCTACAAGCATCGAAAAACGCCTCTTCTGTCGTGTCAAAACTGTCTAAGAGGGCAGGACTAACCCCAACGGAGAAAGGGATCATTAACGATTGCTTAGAAAGTATTAAGGAATCCATCGACGAGCTTAAAGATTCGTTGAGTTCAATGAAAAATTTGGGGGTCAAGGGTGCTGATATGCAAGCTCAGTTAGATGATATTAAGACTTGGGTCAGTGCCGTCATCACAGACGACGTTACTTGCACTGATGGATTCGATGGTCTGAAGGTAAGCACGGCGGTTAAGACCCCCATCAACAATAGCATTGTGTATGTTGCTAGGTTAGCTAGCAATGCTCTGTCGCTCATCGACAGCCTCATCTACTAG

Protein Analysis

209

Amino Acids

22.41

Weight (kDa)

8.74

Isoelectric Point (pI)

26.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 48 - 202 2.8e-36 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 153, 539
AclWI GGATC 1 cut(s) 350
AcsI RAATTY 2 cut(s) 34, 415
AcuI CTGAAG 1 cut(s) 545
AfaI GTAC 1 cut(s) 206
AgsI TTSAA 3 cut(s) 33, 165, 408
AjuI GAANNNNNNNTTGG 2 cut(s) 19, 51
AluBI AGCT 4 cut(s) 238, 388, 446, 587
AluI AGCT 4 cut(s) 238, 388, 446, 587
Alw21I GWGCWC 1 cut(s) 10
AlwI GGATC 1 cut(s) 350
ApoI RAATTY 2 cut(s) 34, 415
ArsI GACNNNNNNTTYG 2 cut(s) 129, 161
AsuNHI GCTAGC 1 cut(s) 587
Bbv12I GWGCWC 1 cut(s) 10
BccI CCATC 4 cut(s) 386, 503, 512, 560
BceAI ACGGC 2 cut(s) 464, 552
BcgI CGANNNNNNTGC 2 cut(s) 222, 256
BfaI CTAG 3 cut(s) 579, 588, 625
BmsI GCATC 2 cut(s) 252, 281
BmtI GCTAGC 1 cut(s) 591
BoxI GACNNNNGTC 1 cut(s) 470
BsaJI CCNNGG 1 cut(s) 105
Bse3DI GCAATG 1 cut(s) 598
BseDI CCNNGG 1 cut(s) 105
BseMI GCAATG 1 cut(s) 598
BseMII CTCAG 1 cut(s) 461
BseRI GAGGAG 1 cut(s) 106
BsiHKAI GWGCWC 1 cut(s) 10
BsiWI CGTACG 1 cut(s) 204
Bsp1286I GDGCHC 1 cut(s) 10
Bsp143I GATC 1 cut(s) 342
BspACI CCGC 2 cut(s) 153, 539
BspCNI CTCAG 1 cut(s) 460
BspHI TCATGA 1 cut(s) 84
BspOI GCTAGC 1 cut(s) 591
BspPI GGATC 1 cut(s) 350
BsrDI GCAATG 1 cut(s) 598
BssECI CCNNGG 1 cut(s) 105
BssMI GATC 1 cut(s) 342
Bst4CI ACNGT 2 cut(s) 106, 306
Bst6I CTCTTC 2 cut(s) 124, 292
BstC8I GCNNGC 3 cut(s) 157, 444, 589
BstDEI CTNAG 3 cut(s) 309, 358, 447
BstDSI CCRYGG 1 cut(s) 105
BstKTI GATC 1 cut(s) 345
BstMBI GATC 1 cut(s) 342
BstMWI GCNNNNNNNGC 2 cut(s) 584, 593
BstNSI RCATGY 1 cut(s) 97
BstPAI GACNNNNGTC 1 cut(s) 470
BtgI CCRYGG 1 cut(s) 105
BtsIMutI CAGTG 2 cut(s) 481, 504
Cac8I GCNNGC 3 cut(s) 157, 444, 589
CciI TCATGA 1 cut(s) 84
Csp6I GTAC 1 cut(s) 205
CviAII CATG 3 cut(s) 44, 85, 94
CviJI RGCY 5 cut(s) 238, 388, 446, 587, 615
CviKI_1 RGCY 5 cut(s) 238, 388, 446, 587, 615
CviQI GTAC 1 cut(s) 205
DdeI CTNAG 3 cut(s) 309, 358, 447
DpnI GATC 1 cut(s) 344
DpnII GATC 1 cut(s) 342
Eam1104I CTCTTC 2 cut(s) 124, 292
EarI CTCTTC 2 cut(s) 124, 292
Eco57I CTGAAG 1 cut(s) 545
FaeI CATG 3 cut(s) 47, 88, 97
FaiI YATR 7 cut(s) 45, 86, 95, 172, 249, 440, 573
FatI CATG 3 cut(s) 43, 84, 93
FspBI CTAG 3 cut(s) 579, 588, 625
Hin1II CATG 3 cut(s) 47, 88, 97
HindIII AAGCTT 1 cut(s) 236
HinfI GANTC 3 cut(s) 374, 395, 513
Hpy188I TCNGA 1 cut(s) 525
Hpy188III TCNNGA 1 cut(s) 85
Hpy99I CGWCG 2 cut(s) 386, 497
HpyAV CCTTC 1 cut(s) 520
HpyCH4III ACNGT 2 cut(s) 106, 306
HpyCH4IV ACGT 2 cut(s) 144, 495
HpyCH4V TGCA 5 cut(s) 97, 159, 243, 442, 504
HpyF10VI GCNNNNNNNGC 2 cut(s) 584, 593
HpyF3I CTNAG 3 cut(s) 309, 358, 447
HpySE526I ACGT 2 cut(s) 144, 495
Hsp92II CATG 3 cut(s) 47, 88, 97
Kzo9I GATC 1 cut(s) 342
LpnPI CCDG 2 cut(s) 169, 303
LweI GCATC 2 cut(s) 252, 281
MaeI CTAG 3 cut(s) 579, 588, 625
MaeII ACGT 2 cut(s) 144, 495
MaeIII GTNAC 2 cut(s) 259, 496
MalI GATC 1 cut(s) 344
MboI GATC 1 cut(s) 342
MboII GAAGA 3 cut(s) 50, 111, 279
MhlI GDGCHC 1 cut(s) 10
MluCI AATT 4 cut(s) 34, 66, 160, 415
MnlI CCTC 9 cut(s) 124, 127, 133, 211, 240, 263, 295, 306, 626
MseI TTAA 5 cut(s) 348, 369, 390, 462, 543
MslI CAYNNNNRTG 1 cut(s) 83
MwoI GCNNNNNNNGC 2 cut(s) 584, 593
NdeII GATC 1 cut(s) 342
NheI GCTAGC 1 cut(s) 587
NlaIII CATG 3 cut(s) 47, 88, 97
NmuCI GTSAC 1 cut(s) 259
NspI RCATGY 1 cut(s) 97
PagI TCATGA 1 cut(s) 84
PfeI GAWTC 3 cut(s) 374, 395, 513
Pfl23II CGTACG 1 cut(s) 204
PshAI GACNNNNGTC 1 cut(s) 470
PspLI CGTACG 1 cut(s) 204
RsaI GTAC 1 cut(s) 206
RsaNI GTAC 1 cut(s) 205
RseI CAYNNNNRTG 1 cut(s) 83
SaqAI TTAA 5 cut(s) 348, 369, 390, 462, 543
Sau3AI GATC 1 cut(s) 342
SduI GDGCHC 1 cut(s) 10
SfaNI GCATC 2 cut(s) 252, 281
SmiMI CAYNNNNRTG 1 cut(s) 83
Sse9I AATT 4 cut(s) 34, 66, 160, 415
SsiI CCGC 2 cut(s) 153, 539
SspMI CTAG 3 cut(s) 579, 588, 625
TaaI ACNGT 2 cut(s) 106, 306
TaiI ACGT 2 cut(s) 147, 498
TaqI TCGA 5 cut(s) 232, 275, 381, 516, 609
TasI AATT 4 cut(s) 34, 66, 160, 415
TfiI GAWTC 3 cut(s) 374, 395, 513
Tru1I TTAA 5 cut(s) 348, 369, 390, 462, 543
Tru9I TTAA 5 cut(s) 348, 369, 390, 462, 543
TscAI CASTG 2 cut(s) 481, 511
TseFI GTSAC 1 cut(s) 259
Tsp45I GTSAC 1 cut(s) 259
TspDTI ATGAA 1 cut(s) 425
TspGWI ACGGA 2 cut(s) 247, 347
TspRI CASTG 2 cut(s) 481, 511
XapI RAATTY 2 cut(s) 34, 415
XceI RCATGY 1 cut(s) 97
XspI CTAG 3 cut(s) 579, 588, 625
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.