FvH4_7g27830

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0005396
Physical Location & Seq
Forward (+)
20727658 .. 20728761
1104 bp
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UTR
Exon/CDS
Intron
FvH4_7g27830.t1

Sequence Viewer

Length: 576 bp
ATGGGAGCTCCCACATTTCTCTTTGCTCTTCTTCTATTCATCCTCTTCATCAACACAACCACGGCGACTTCTTCTTCGAGCCGTACCTACAAAACGTACGTCAAAACAGCCTGCAACTCCACCACGTACCCACAAATCTGCTACAAATCCCTCTCCTCTTACGCCACATCCATCAAATCAAACCCTCAGAAGCTCTGCAGCTACGCCCTCTCCGTAACCCTAAAAGCAGCGAAAACCTCGGCTTCCACGGTGTCGAAGCTGGCCAAGAAGAAAGGAATAACGAAAGCGGAGGCCGGTGTGGTGGGGGCTTGCATTGAGACCATCAAGGACTCCGTCGACGAGCTCAAACAGTCGGTGACTGCCATGAAAAACTTGGGAGGTGCTGATATGCAATATCAATTGGATAACATAAAGACTTGGGTTAGTGCTTCGCTGACGGATGAGGATACCTGTACGGATGGATTCGATGAGCAGAAGGTAAGTACGGCGGTGAAGAACACCATTAGAAAGAGCATTCTCACTGTTGCTAGATTGGCTAGCAATGCCTTGTCTCTCATCGACAACCTCAATTACTAG

Protein Analysis

192

Amino Acids

20.56

Weight (kDa)

9.22

Isoelectric Point (pI)

30.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 33 - 185 1.2e-35 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 336
AciI CCGC 2 cut(s) 287, 488
AcoI YGGCCR 1 cut(s) 261
AfaI GTAC 5 cut(s) 85, 98, 128, 454, 484
AluBI AGCT 5 cut(s) 8, 193, 201, 259, 343
AluI AGCT 5 cut(s) 8, 193, 201, 259, 343
Alw21I GWGCWC 2 cut(s) 10, 345
Alw26I GTCTC 2 cut(s) 311, 555
AoxI GGCC 2 cut(s) 261, 291
ApeKI GCWGC 2 cut(s) 198, 227
ArsI GACNNNNNNTTYG 4 cut(s) 58, 84, 90, 116
AsuHPI GGTGA 2 cut(s) 367, 502
AsuNHI GCTAGC 1 cut(s) 536
BalI TGGCCA 1 cut(s) 263
BanII GRGCYC 2 cut(s) 10, 345
Bbv12I GWGCWC 2 cut(s) 10, 345
BbvI GCAGC 2 cut(s) 210, 239
BccI CCATC 3 cut(s) 179, 329, 452
BceAI ACGGC 3 cut(s) 66, 78, 501
BciVI GTATCC 1 cut(s) 439
BcoDI GTCTC 2 cut(s) 311, 555
BfaI CTAG 3 cut(s) 528, 537, 574
BfmI CTRYAG 1 cut(s) 196
BfuI GTATCC 1 cut(s) 439
BisI GCNGC 2 cut(s) 199, 228
BlsI GCNGC 2 cut(s) 200, 229
BmtI GCTAGC 1 cut(s) 540
BsaAI YACGTR 1 cut(s) 126
BsaI GGTCTC 1 cut(s) 311
BsaJI CCNNGG 3 cut(s) 60, 237, 246
BsaXI ACNNNNNCTCC 2 cut(s) 194, 224
Bse118I RCCGGY 1 cut(s) 293
Bse3DI GCAATG 1 cut(s) 547
BseDI CCNNGG 3 cut(s) 60, 237, 246
BseGI GGATG 4 cut(s) 39, 167, 445, 463
BseMI GCAATG 1 cut(s) 547
BseMII CTCAG 1 cut(s) 200
BseRI GAGGAG 1 cut(s) 145
BseXI GCAGC 2 cut(s) 210, 239
BshFI GGCC 2 cut(s) 263, 293
BsiHKAI GWGCWC 2 cut(s) 10, 345
BsiSI CCGG 1 cut(s) 294
BsiWI CGTACG 1 cut(s) 96
BsmAI GTCTC 2 cut(s) 311, 555
BsmI GAATGC 1 cut(s) 513
BsnI GGCC 2 cut(s) 263, 293
Bso31I GGTCTC 1 cut(s) 311
Bsp1286I GDGCHC 2 cut(s) 10, 345
BspACI CCGC 2 cut(s) 287, 488
BspANI GGCC 2 cut(s) 263, 293
BspCNI CTCAG 1 cut(s) 199
BspMAI CTGCAG 1 cut(s) 200
BspOI GCTAGC 1 cut(s) 540
BspQI GCTCTTC 1 cut(s) 33
BspTNI GGTCTC 1 cut(s) 311
BsrDI GCAATG 1 cut(s) 547
BsrFI RCCGGY 1 cut(s) 293
BssAI RCCGGY 1 cut(s) 293
BssECI CCNNGG 3 cut(s) 60, 237, 246
Bst4CI ACNGT 3 cut(s) 250, 351, 523
Bst6I CTCTTC 2 cut(s) 33, 50
BstBAI YACGTR 1 cut(s) 126
BstC8I GCNNGC 4 cut(s) 112, 261, 310, 538
BstDEI CTNAG 1 cut(s) 186
BstDSI CCRYGG 2 cut(s) 60, 246
BstF5I GGATG 4 cut(s) 39, 167, 445, 463
BstMAI GTCTC 2 cut(s) 311, 555
BstMWI GCNNNNNNNGC 2 cut(s) 533, 542
BstSFI CTRYAG 1 cut(s) 196
BstV1I GCAGC 2 cut(s) 210, 239
BsuI GTATCC 1 cut(s) 439
BsuRI GGCC 2 cut(s) 263, 293
BtgI CCRYGG 2 cut(s) 60, 246
BtsCI GGATG 4 cut(s) 39, 167, 445, 463
BtsIMutI CAGTG 1 cut(s) 519
Cac8I GCNNGC 4 cut(s) 112, 261, 310, 538
Cfr10I RCCGGY 1 cut(s) 293
Csp6I GTAC 5 cut(s) 84, 97, 127, 453, 483
CviAII CATG 1 cut(s) 364
CviQI GTAC 5 cut(s) 84, 97, 127, 453, 483
DdeI CTNAG 1 cut(s) 186
EaeI YGGCCR 1 cut(s) 261
Eam1104I CTCTTC 2 cut(s) 33, 50
EarI CTCTTC 2 cut(s) 33, 50
Ecl136II GAGCTC 2 cut(s) 8, 343
Eco24I GRGCYC 2 cut(s) 10, 345
Eco31I GGTCTC 1 cut(s) 311
Eco53kI GAGCTC 2 cut(s) 8, 343
EcoICRI GAGCTC 2 cut(s) 8, 343
EcoT38I GRGCYC 2 cut(s) 10, 345
FaeI CATG 1 cut(s) 367
FaiI YATR 3 cut(s) 365, 389, 410
FatI CATG 1 cut(s) 363
FblI GTMKAC 1 cut(s) 336
Fnu4HI GCNGC 2 cut(s) 199, 228
FokI GGATG 4 cut(s) 26, 154, 452, 470
FriOI GRGCYC 2 cut(s) 10, 345
Fsp4HI GCNGC 2 cut(s) 199, 228
FspBI CTAG 3 cut(s) 528, 537, 574
GluI GCNGC 2 cut(s) 199, 228
HaeIII GGCC 2 cut(s) 263, 293
HapII CCGG 1 cut(s) 294
Hin1II CATG 1 cut(s) 367
HincII GTYRAC 1 cut(s) 337
HindII GTYRAC 1 cut(s) 337
HinfI GANTC 2 cut(s) 329, 462
HpaII CCGG 1 cut(s) 294
HphI GGTGA 2 cut(s) 367, 502
Hpy166II GTNNAC 1 cut(s) 337
Hpy188I TCNGA 1 cut(s) 189
Hpy8I GTNNAC 1 cut(s) 337
Hpy99I CGWCG 2 cut(s) 338, 341
HpyAV CCTTC 1 cut(s) 469
HpyCH4III ACNGT 3 cut(s) 250, 351, 523
HpyCH4IV ACGT 3 cut(s) 95, 99, 125
HpyCH4V TGCA 4 cut(s) 114, 198, 312, 391
HpyF10VI GCNNNNNNNGC 2 cut(s) 533, 542
HpyF3I CTNAG 1 cut(s) 186
HpySE526I ACGT 3 cut(s) 95, 99, 125
Hsp92II CATG 1 cut(s) 367
LguI GCTCTTC 1 cut(s) 33
LmnI GCTCC 2 cut(s) 5, 13
LpnPI CCDG 4 cut(s) 124, 245, 307, 463
Lsp1109I GCAGC 2 cut(s) 210, 239
MaeI CTAG 3 cut(s) 528, 537, 574
MaeII ACGT 3 cut(s) 95, 99, 125
MaeIII GTNAC 2 cut(s) 214, 355
MboII GAAGA 7 cut(s) 20, 23, 37, 63, 66, 280, 505
MfeI CAATTG 1 cut(s) 398
MhlI GDGCHC 2 cut(s) 10, 345
MlsI TGGCCA 1 cut(s) 263
MluCI AATT 2 cut(s) 398, 568
MluNI TGGCCA 1 cut(s) 263
MlyI GAGTC 1 cut(s) 323
Mox20I TGGCCA 1 cut(s) 263
MscI TGGCCA 1 cut(s) 263
Msp20I TGGCCA 1 cut(s) 263
MspI CCGG 1 cut(s) 294
MunI CAATTG 1 cut(s) 398
Mva1269I GAATGC 1 cut(s) 513
MwoI GCNNNNNNNGC 2 cut(s) 533, 542
NheI GCTAGC 1 cut(s) 536
NlaIII CATG 1 cut(s) 367
NmeAIII GCCGAG 1 cut(s) 218
NmuCI GTSAC 1 cut(s) 355
PciSI GCTCTTC 1 cut(s) 33
PcsI WCGNNNNNNNCGW 1 cut(s) 210
PctI GAATGC 1 cut(s) 513
PfeI GAWTC 1 cut(s) 462
Pfl23II CGTACG 1 cut(s) 96
PflFI GACNNNGTC 1 cut(s) 332
PkrI GCNGC 2 cut(s) 200, 229
PleI GAGTC 1 cut(s) 323
PpsI GAGTC 1 cut(s) 323
Ppu21I YACGTR 1 cut(s) 126
Psp124BI GAGCTC 2 cut(s) 10, 345
PspLI CGTACG 1 cut(s) 96
PstI CTGCAG 1 cut(s) 200
PsyI GACNNNGTC 1 cut(s) 332
RsaI GTAC 5 cut(s) 85, 98, 128, 454, 484
RsaNI GTAC 5 cut(s) 84, 97, 127, 453, 483
SacI GAGCTC 2 cut(s) 10, 345
SalI GTCGAC 1 cut(s) 335
SapI GCTCTTC 1 cut(s) 33
SatI GCNGC 2 cut(s) 199, 228
SchI GAGTC 1 cut(s) 323
SduI GDGCHC 2 cut(s) 10, 345
SfcI CTRYAG 1 cut(s) 196
SgrDI CGTCGACG 1 cut(s) 335
Sse9I AATT 2 cut(s) 398, 568
SsiI CCGC 2 cut(s) 287, 488
SspMI CTAG 3 cut(s) 528, 537, 574
SstI GAGCTC 2 cut(s) 10, 345
TaaI ACNGT 3 cut(s) 250, 351, 523
TaiI ACGT 3 cut(s) 98, 102, 128
TaqI TCGA 5 cut(s) 77, 254, 336, 465, 558
TasI AATT 2 cut(s) 398, 568
TfiI GAWTC 1 cut(s) 462
TscAI CASTG 1 cut(s) 526
TseFI GTSAC 1 cut(s) 355
TseI GCWGC 2 cut(s) 198, 227
Tsp45I GTSAC 1 cut(s) 355
TspDTI ATGAA 3 cut(s) 28, 37, 380
TspGWI ACGGA 4 cut(s) 202, 322, 452, 470
TspRI CASTG 1 cut(s) 526
Tth111I GACNNNGTC 1 cut(s) 332
XcmI CCANNNNNNNNNTGG 1 cut(s) 370
XmiI GTMKAC 1 cut(s) 336
XspI CTAG 3 cut(s) 528, 537, 574
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.