Belongs to the protein kinase superfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 44 |
| AccB1I | GGYRCC | 1 cut(s) | 190 |
| AciI | CCGC | 2 cut(s) | 16, 289 |
| AclWI | GGATC | 1 cut(s) | 204 |
| AcsI | RAATTY | 1 cut(s) | 556 |
| AcuI | CTGAAG | 3 cut(s) | 248, 561, 755 |
| AcyI | GRCGYC | 1 cut(s) | 41 |
| AdeI | CACNNNGTG | 1 cut(s) | 132 |
| AfaI | GTAC | 3 cut(s) | 50, 86, 113 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 142, 375, 376 |
| AflII | CTTAAG | 1 cut(s) | 506 |
| AgsI | TTSAA | 3 cut(s) | 109, 700, 816 |
| AjnI | CCWGG | 3 cut(s) | 44, 312, 497 |
| AleI | CACNNNNGTG | 1 cut(s) | 756 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 438, 470 |
| AluBI | AGCT | 6 cut(s) | 162, 494, 553, 593, 656, 803 |
| AluI | AGCT | 6 cut(s) | 162, 494, 553, 593, 656, 803 |
| Alw21I | GWGCWC | 1 cut(s) | 763 |
| Alw26I | GTCTC | 1 cut(s) | 251 |
| AlwI | GGATC | 1 cut(s) | 204 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 226 |
| AoxI | GGCC | 1 cut(s) | 324 |
| ApeKI | GCWGC | 4 cut(s) | 73, 286, 485, 494 |
| ApoI | RAATTY | 1 cut(s) | 556 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 721, 753 |
| AspLEI | GCGC | 2 cut(s) | 505, 797 |
| AspS9I | GGNCC | 1 cut(s) | 324 |
| AsuHPI | GGTGA | 2 cut(s) | 118, 455 |
| BanI | GGYRCC | 1 cut(s) | 190 |
| BanII | GRGCYC | 1 cut(s) | 36 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 104, 136 |
| BauI | CACGAG | 2 cut(s) | 35, 248 |
| Bbv12I | GWGCWC | 1 cut(s) | 763 |
| BbvI | GCAGC | 4 cut(s) | 60, 273, 481, 497 |
| BccI | CCATC | 3 cut(s) | 181, 465, 850 |
| BceAI | ACGGC | 4 cut(s) | 159, 252, 319, 332 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 188, 222 |
| BciT130I | CCWGG | 3 cut(s) | 46, 314, 499 |
| BcoDI | GTCTC | 1 cut(s) | 251 |
| BfaI | CTAG | 2 cut(s) | 653, 779 |
| BfmI | CTRYAG | 2 cut(s) | 318, 798 |
| BfoI | RGCGCY | 2 cut(s) | 506, 798 |
| BfrI | CTTAAG | 1 cut(s) | 506 |
| BisI | GCNGC | 5 cut(s) | 16, 74, 287, 486, 495 |
| BlpI | GCTNAGC | 2 cut(s) | 92, 158 |
| BlsI | GCNGC | 5 cut(s) | 17, 75, 288, 487, 496 |
| BmcAI | AGTACT | 1 cut(s) | 86 |
| Bme1390I | CCNGG | 3 cut(s) | 46, 314, 499 |
| BmgT120I | GGNCC | 1 cut(s) | 324 |
| BmiI | GGNNCC | 3 cut(s) | 192, 325, 585 |
| BmrFI | CCNGG | 3 cut(s) | 46, 314, 499 |
| BmsI | GCATC | 1 cut(s) | 186 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 92, 158 |
| BsaHI | GRCGYC | 1 cut(s) | 41 |
| BsaJI | CCNNGG | 1 cut(s) | 498 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 142, 375, 376 |
| Bse118I | RCCGGY | 1 cut(s) | 298 |
| Bse1I | ACTGG | 3 cut(s) | 435, 756, 853 |
| Bse3DI | GCAATG | 1 cut(s) | 429 |
| BseBI | CCWGG | 3 cut(s) | 46, 314, 499 |
| BseDI | CCNNGG | 1 cut(s) | 498 |
| BseGI | GGATG | 2 cut(s) | 53, 186 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 142, 375, 376 |
| BseMI | GCAATG | 1 cut(s) | 429 |
| BseMII | CTCAG | 1 cut(s) | 172 |
| BseNI | ACTGG | 3 cut(s) | 435, 756, 853 |
| BseXI | GCAGC | 4 cut(s) | 60, 273, 481, 497 |
| Bsh1285I | CGRYCG | 1 cut(s) | 30 |
| BshFI | GGCC | 1 cut(s) | 326 |
| BshNI | GGYRCC | 1 cut(s) | 190 |
| BsiEI | CGRYCG | 1 cut(s) | 30 |
| BsiHKAI | GWGCWC | 1 cut(s) | 763 |
| BsiSI | CCGG | 1 cut(s) | 299 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 142, 375, 376 |
| BsmAI | GTCTC | 1 cut(s) | 251 |
| BsnI | GGCC | 1 cut(s) | 326 |
| Bsp1286I | GDGCHC | 2 cut(s) | 36, 763 |
| Bsp143I | GATC | 4 cut(s) | 27, 117, 209, 231 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 92, 158 |
| BspACI | CCGC | 2 cut(s) | 16, 289 |
| BspANI | GGCC | 1 cut(s) | 326 |
| BspCNI | CTCAG | 1 cut(s) | 171 |
| BspLI | GGNNCC | 3 cut(s) | 192, 325, 585 |
| BspPI | GGATC | 1 cut(s) | 204 |
| BspQI | GCTCTTC | 1 cut(s) | 5 |
| BspT107I | GGYRCC | 1 cut(s) | 190 |
| BspTI | CTTAAG | 1 cut(s) | 506 |
| BsrDI | GCAATG | 1 cut(s) | 429 |
| BsrFI | RCCGGY | 1 cut(s) | 298 |
| BsrI | ACTGG | 3 cut(s) | 435, 756, 853 |
| BssAI | RCCGGY | 1 cut(s) | 298 |
| BssECI | CCNNGG | 1 cut(s) | 498 |
| BssMI | GATC | 4 cut(s) | 27, 117, 209, 231 |
| BssNI | GRCGYC | 1 cut(s) | 41 |
| BssSI | CACGAG | 2 cut(s) | 35, 248 |
| Bst2BI | CACGAG | 2 cut(s) | 35, 248 |
| Bst2UI | CCWGG | 3 cut(s) | 46, 314, 499 |
| Bst4CI | ACNGT | 2 cut(s) | 874, 896 |
| Bst6I | CTCTTC | 2 cut(s) | 5, 774 |
| BstACI | GRCGYC | 1 cut(s) | 41 |
| BstAFI | CTTAAG | 1 cut(s) | 506 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 494 |
| BstDEI | CTNAG | 2 cut(s) | 92, 158 |
| BstF5I | GGATG | 2 cut(s) | 53, 186 |
| BstH2I | RGCGCY | 2 cut(s) | 506, 798 |
| BstHHI | GCGC | 2 cut(s) | 505, 797 |
| BstKTI | GATC | 4 cut(s) | 30, 120, 212, 234 |
| BstMAI | GTCTC | 1 cut(s) | 251 |
| BstMBI | GATC | 4 cut(s) | 27, 117, 209, 231 |
| BstMCI | CGRYCG | 1 cut(s) | 30 |
| BstMWI | GCNNNNNNNGC | 9 cut(s) | 102, 295, 323, 353, 491, 494, 500, 590, 794 |
| BstNI | CCWGG | 3 cut(s) | 46, 314, 499 |
| BstSCI | CCNGG | 3 cut(s) | 44, 312, 497 |
| BstSFI | CTRYAG | 2 cut(s) | 318, 798 |
| BstV1I | GCAGC | 4 cut(s) | 60, 273, 481, 497 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 464, 739 |
| BsuRI | GGCC | 1 cut(s) | 326 |
| BtgZI | GCGATG | 1 cut(s) | 151 |
| BtsCI | GGATG | 2 cut(s) | 53, 186 |
| BtsI | GCAGTG | 1 cut(s) | 342 |
| BtsIMutI | CAGTG | 5 cut(s) | 342, 442, 519, 763, 901 |
| CaiI | CAGNNNCTG | 1 cut(s) | 226 |
| CfoI | GCGC | 2 cut(s) | 505, 797 |
| Cfr10I | RCCGGY | 1 cut(s) | 298 |
| Cfr13I | GGNCC | 1 cut(s) | 324 |
| CseI | GACGC | 1 cut(s) | 409 |
| Csp6I | GTAC | 3 cut(s) | 49, 85, 112 |
| CviAII | CATG | 3 cut(s) | 130, 139, 625 |
| CviQI | GTAC | 3 cut(s) | 49, 85, 112 |
| DdeI | CTNAG | 2 cut(s) | 92, 158 |
| DpnI | GATC | 4 cut(s) | 29, 119, 211, 233 |
| DpnII | GATC | 4 cut(s) | 27, 117, 209, 231 |
| DraIII | CACNNNGTG | 1 cut(s) | 132 |
| Eam1104I | CTCTTC | 2 cut(s) | 5, 774 |
| EarI | CTCTTC | 2 cut(s) | 5, 774 |
| Eco24I | GRGCYC | 1 cut(s) | 36 |
| Eco57I | CTGAAG | 3 cut(s) | 248, 561, 755 |
| EcoRI | GAATTC | 1 cut(s) | 556 |
| EcoRII | CCWGG | 3 cut(s) | 44, 312, 497 |
| EcoT38I | GRGCYC | 1 cut(s) | 36 |
| FaeI | CATG | 3 cut(s) | 133, 142, 628 |
| FalI | AAGNNNNNCTT | 1 cut(s) | 34 |
| FatI | CATG | 3 cut(s) | 129, 138, 624 |
| Fnu4HI | GCNGC | 5 cut(s) | 16, 74, 287, 486, 495 |
| FokI | GGATG | 2 cut(s) | 40, 193 |
| FriOI | GRGCYC | 1 cut(s) | 36 |
| Fsp4HI | GCNGC | 5 cut(s) | 16, 74, 287, 486, 495 |
| FspBI | CTAG | 2 cut(s) | 653, 779 |
| GlaI | GCGC | 2 cut(s) | 504, 796 |
| GluI | GCNGC | 5 cut(s) | 16, 74, 287, 486, 495 |
| HaeII | RGCGCY | 2 cut(s) | 506, 798 |
| HaeIII | GGCC | 1 cut(s) | 326 |
| HapII | CCGG | 1 cut(s) | 299 |
| HgaI | GACGC | 1 cut(s) | 409 |
| HhaI | GCGC | 2 cut(s) | 505, 797 |
| Hin1I | GRCGYC | 1 cut(s) | 41 |
| Hin1II | CATG | 3 cut(s) | 133, 142, 628 |
| Hin6I | GCGC | 2 cut(s) | 503, 795 |
| HinP1I | GCGC | 2 cut(s) | 503, 795 |
| HinfI | GANTC | 2 cut(s) | 244, 766 |
| HpaII | CCGG | 1 cut(s) | 299 |
| HphI | GGTGA | 2 cut(s) | 118, 455 |
| Hpy166II | GTNNAC | 2 cut(s) | 24, 446 |
| Hpy188I | TCNGA | 5 cut(s) | 228, 541, 588, 729, 765 |
| Hpy188III | TCNNGA | 5 cut(s) | 25, 37, 676, 745, 820 |
| Hpy8I | GTNNAC | 2 cut(s) | 24, 446 |
| HpyAV | CCTTC | 1 cut(s) | 536 |
| HpyCH4III | ACNGT | 2 cut(s) | 874, 896 |
| HpyCH4IV | ACGT | 1 cut(s) | 41 |
| HpyCH4V | TGCA | 4 cut(s) | 199, 614, 662, 788 |
| HpyF10VI | GCNNNNNNNGC | 9 cut(s) | 102, 295, 323, 353, 491, 494, 500, 590, 794 |
| HpyF3I | CTNAG | 2 cut(s) | 92, 158 |
| HpySE526I | ACGT | 1 cut(s) | 41 |
| Hsp92I | GRCGYC | 1 cut(s) | 41 |
| Hsp92II | CATG | 3 cut(s) | 133, 142, 628 |
| HspAI | GCGC | 2 cut(s) | 503, 795 |
| Kzo9I | GATC | 4 cut(s) | 27, 117, 209, 231 |
| LguI | GCTCTTC | 1 cut(s) | 5 |
| LmnI | GCTCC | 1 cut(s) | 589 |
| Lsp1109I | GCAGC | 4 cut(s) | 60, 273, 481, 497 |
| LweI | GCATC | 1 cut(s) | 186 |
| MaeI | CTAG | 2 cut(s) | 653, 779 |
| MaeII | ACGT | 1 cut(s) | 41 |
| MaeIII | GTNAC | 4 cut(s) | 37, 415, 742, 844 |
| MalI | GATC | 4 cut(s) | 29, 119, 211, 233 |
| MboI | GATC | 4 cut(s) | 27, 117, 209, 231 |
| MboII | GAAGA | 9 cut(s) | 22, 112, 196, 241, 331, 528, 641, 761, 916 |
| MfeI | CAATTG | 1 cut(s) | 171 |
| MhlI | GDGCHC | 2 cut(s) | 36, 763 |
| MluCI | AATT | 5 cut(s) | 171, 474, 556, 700, 885 |
| MlyI | GAGTC | 2 cut(s) | 253, 760 |
| MmeI | TCCRAC | 1 cut(s) | 81 |
| MnlI | CCTC | 3 cut(s) | 134, 373, 578 |
| MseI | TTAA | 3 cut(s) | 309, 473, 507 |
| MslI | CAYNNNNRTG | 1 cut(s) | 756 |
| MspA1I | CMGCKG | 1 cut(s) | 494 |
| MspCI | CTTAAG | 1 cut(s) | 506 |
| MspI | CCGG | 1 cut(s) | 299 |
| MspR9I | CCNGG | 3 cut(s) | 46, 314, 499 |
| MunI | CAATTG | 1 cut(s) | 171 |
| MvaI | CCWGG | 3 cut(s) | 46, 314, 499 |
| MwoI | GCNNNNNNNGC | 9 cut(s) | 102, 295, 323, 353, 491, 494, 500, 590, 794 |
| NdeII | GATC | 4 cut(s) | 27, 117, 209, 231 |
| NlaIII | CATG | 3 cut(s) | 133, 142, 628 |
| NlaIV | GGNNCC | 3 cut(s) | 192, 325, 585 |
| NmuCI | GTSAC | 3 cut(s) | 37, 742, 844 |
| OliI | CACNNNNGTG | 1 cut(s) | 756 |
| PciSI | GCTCTTC | 1 cut(s) | 5 |
| PkrI | GCNGC | 5 cut(s) | 17, 75, 288, 487, 496 |
| Ple19I | CGATCG | 1 cut(s) | 30 |
| PleI | GAGTC | 2 cut(s) | 252, 760 |
| PpsI | GAGTC | 2 cut(s) | 252, 760 |
| Psp6I | CCWGG | 3 cut(s) | 44, 312, 497 |
| PspGI | CCWGG | 3 cut(s) | 44, 312, 497 |
| PspN4I | GGNNCC | 3 cut(s) | 192, 325, 585 |
| PspPI | GGNCC | 1 cut(s) | 324 |
| PstNI | CAGNNNCTG | 1 cut(s) | 226 |
| PvuI | CGATCG | 1 cut(s) | 30 |
| PvuII | CAGCTG | 1 cut(s) | 494 |
| RsaI | GTAC | 3 cut(s) | 50, 86, 113 |
| RsaNI | GTAC | 3 cut(s) | 49, 85, 112 |
| RseI | CAYNNNNRTG | 1 cut(s) | 756 |
| SapI | GCTCTTC | 1 cut(s) | 5 |
| SaqAI | TTAA | 3 cut(s) | 309, 473, 507 |
| SatI | GCNGC | 5 cut(s) | 16, 74, 287, 486, 495 |
| Sau3AI | GATC | 4 cut(s) | 27, 117, 209, 231 |
| Sau96I | GGNCC | 1 cut(s) | 324 |
| ScaI | AGTACT | 1 cut(s) | 86 |
| SchI | GAGTC | 2 cut(s) | 253, 760 |
| ScrFI | CCNGG | 3 cut(s) | 46, 314, 499 |
| SduI | GDGCHC | 2 cut(s) | 36, 763 |
| SfaNI | GCATC | 1 cut(s) | 186 |
| SfcI | CTRYAG | 2 cut(s) | 318, 798 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 756 |
| SmlI | CTYRAG | 1 cut(s) | 506 |
| SmoI | CTYRAG | 1 cut(s) | 506 |
| Sse9I | AATT | 5 cut(s) | 171, 474, 556, 700, 885 |
| SsiI | CCGC | 2 cut(s) | 16, 289 |
| SspMI | CTAG | 2 cut(s) | 653, 779 |
| StyD4I | CCNGG | 3 cut(s) | 44, 312, 497 |
| TaaI | ACNGT | 2 cut(s) | 874, 896 |
| TaiI | ACGT | 1 cut(s) | 44 |
| TaqI | TCGA | 2 cut(s) | 208, 560 |
| TasI | AATT | 5 cut(s) | 171, 474, 556, 700, 885 |
| TatI | WGTACW | 1 cut(s) | 84 |
| TauI | GCSGC | 1 cut(s) | 18 |
| Tru1I | TTAA | 3 cut(s) | 309, 473, 507 |
| Tru9I | TTAA | 3 cut(s) | 309, 473, 507 |
| TscAI | CASTG | 5 cut(s) | 349, 442, 519, 763, 901 |
| TseFI | GTSAC | 3 cut(s) | 37, 742, 844 |
| TseI | GCWGC | 4 cut(s) | 73, 286, 485, 494 |
| Tsp45I | GTSAC | 3 cut(s) | 37, 742, 844 |
| TspDTI | ATGAA | 3 cut(s) | 17, 197, 578 |
| TspRI | CASTG | 5 cut(s) | 349, 442, 519, 763, 901 |
| Vha464I | CTTAAG | 1 cut(s) | 506 |
| XapI | RAATTY | 1 cut(s) | 556 |
| XspI | CTAG | 2 cut(s) | 653, 779 |
| ZraI | GACGTC | 1 cut(s) | 42 |
| ZrmI | AGTACT | 1 cut(s) | 86 |