MD06G1052900.v1.1
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0000445
Physical Location & Seq
Forward (+)
7665814 .. 7666357
544 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1052900.v1.1.491

Sequence Viewer

Length: 477 bp
ATGTTTCATGGCCTGGAATATATGCACAGAAACGGCTATTTCCACCGTGATTTGAAGCCCGCTAACGTCTTAGTTAACGATGGTGCACGTGTGGTCAAGACTGCGATTCGCCCCCTCCCTACACAGACTACGTCACCACTCGCCCTTATCGAGCTCCTGAGAGCTGCCTTCAAGATTTGCAGCGTCATTGGCAGTCCAACTTGGGAGACATGGCCAGAGGGAGAGCTTCTTGCCCAAAACTTGAACTATGAGCTTCCACAACTTCACGGTGTTGATCTATCTGCGATGATTCCTTGGGCAAGCAGATCTGTCATCCAGCTGATTTCTTCACTTCGTTCTTGGGACCCTTCCGCAAGGCCTGCTGCTGCTGAAGCGCTCAAGCATCCCTTCTTCGTTGGCAACCACAAGATTCCACGCGCCATCCCTTTGAGACAACGCAATATTCTGCCTGCGTCGAAACCTCTTATTTTCATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

159

Amino Acids

17.74

Weight (kDa)

9.91

Isoelectric Point (pI)

43.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 2 - 28 4.1e-07 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000445)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G19110 AT4G19110 AT4G19110 AT5G45430 AT5G45430
fragaria_vesca FvH4_2g16890 FvH4_5g19220 FvH4_5g19340 FvH4_5g19350 FvH4_5g19360 FvH4_5g23910 FvH4_7g27810
malus_domestica MD00G1001400.v1.1 MD04G1058900.v1.1 MD04G1059300.v1.1 MD04G1059500.v1.1 MD04G1059600.v1.1 MD04G1059800.v1.1 MD04G1061700.v1.1 MD04G1061800.v1.1 MD06G1014800.v1.1 MD06G1052900.v1.1 MD06G1055500.v1.1
prunus_persica Prupe.5G021300_v2.0.a1 Prupe.5G065900_v2.0.a1 Prupe.5G066500_v2.0.a1
pyrus_communis pycom06g04440
rosa_chinensis RchiOBHm_Chr1g0374681 RchiOBHm_Chr2g0120471 RchiOBHm_Chr2g0120711 RchiOBHm_Chr7g0203821 RchiOBHm_Chr7g0203861 RchiOBHm_Chr7g0208401 RchiOBHm_Chr7g0209011 RchiOBHm_Chr7g0213341
rosa_laevigata RLG00000001348 RLG00000002813 RLG00000003173 RLG00000003212 RLG00000003503 RLG00000022835 RLG00000026723 RLG00000035532
rosa_multiflora Rmu_co7960007.1_g000001 Rmu_co7963796.1_g000001 Rmu_co8519951.1_g000002 Rmu_sc0002038.1_g000009 Rmu_sc0004947.1_g000015 Rmu_sc0007490.1_g000005 Rmu_sc0011180.1_g000003 Rmu_sc0015273.1_g000013 Rmu_ssc0000190.1_g000014 Rmu_ssc0000372.1_g000031
rosa_roxburghii Rroxscaffold_3G00246080 Rroxscaffold_3G00249860 Rroxscaffold_3G00249870 Rroxscaffold_3G00253500 Rroxscaffold_4G00283340
rosa_rugosa Rorug01G0384300 Rorug07G0079000 Rorug07G0079000 Rorug07G0079000 Rorug07G0108300 Rorug07G0141200
rosa_samantha Rh1AG395700 Rh1BG359900 Rh1DG006600 Rh1DG390900 Rh2AG287100 Rh3BG335800 Rh3CG321100 Rh5DG116800 Rh7AG273000 Rh7BG207600 Rh7BG207800 Rh7BG237700 Rh7BG268400 Rh7CG221100 Rh7CG221300 Rh7CG254000 Rh7CG257900 Rh7CG292200 Rh7DG215700 Rh7DG215900 Rh7DG244500 Rh7DG249800 Rh7DG280700
rosa_wichuraiana Rw0G010370 Rw1G035090 Rw2G023020 Rw7G018200 Rw7G020570 Rw7G023690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 417
AciI CCGC 2 cut(s) 60, 351
AcoI YGGCCR 1 cut(s) 212
AcuI CTGAAG 1 cut(s) 390
AcvI CACGTG 1 cut(s) 89
AfeI AGCGCT 1 cut(s) 375
AflIII ACRYGT 1 cut(s) 88
AgsI TTSAA 3 cut(s) 55, 172, 244
AjnI CCWGG 1 cut(s) 12
AluBI AGCT 5 cut(s) 154, 164, 226, 253, 319
AluI AGCT 5 cut(s) 154, 164, 226, 253, 319
Alw21I GWGCWC 2 cut(s) 88, 156
Alw26I GTCTC 2 cut(s) 200, 424
Alw44I GTGCAC 1 cut(s) 84
Aor51HI AGCGCT 1 cut(s) 375
AoxI GGCC 3 cut(s) 10, 212, 356
ApaLI GTGCAC 1 cut(s) 84
ApeKI GCWGC 4 cut(s) 164, 180, 362, 365
AspLEI GCGC 2 cut(s) 376, 419
AspS9I GGNCC 1 cut(s) 343
AsuHPI GGTGA 1 cut(s) 126
AvaII GGWCC 1 cut(s) 343
BaeGI GKGCMC 1 cut(s) 88
BalI TGGCCA 1 cut(s) 214
BanII GRGCYC 1 cut(s) 156
BbrPI CACGTG 1 cut(s) 89
Bbv12I GWGCWC 2 cut(s) 88, 156
BbvI GCAGC 4 cut(s) 151, 192, 349, 352
BccI CCATC 2 cut(s) 74, 428
BceAI ACGGC 1 cut(s) 49
BciT130I CCWGG 1 cut(s) 14
BcoDI GTCTC 2 cut(s) 200, 424
BfoI RGCGCY 1 cut(s) 377
BglII AGATCT 1 cut(s) 305
BisI GCNGC 4 cut(s) 165, 181, 363, 366
BlsI GCNGC 4 cut(s) 166, 182, 364, 367
Bme1390I CCNGG 1 cut(s) 14
Bme18I GGWCC 1 cut(s) 343
BmgT120I GGNCC 1 cut(s) 343
BmiI GGNNCC 2 cut(s) 344, 345
BmrFI CCNGG 1 cut(s) 14
BmsI GCATC 1 cut(s) 391
BpuEI CTTGAG 1 cut(s) 362
BsaAI YACGTR 1 cut(s) 89
BsaJI CCNNGG 1 cut(s) 293
BseBI CCWGG 1 cut(s) 14
BseDI CCNNGG 1 cut(s) 293
BseGI GGATG 3 cut(s) 312, 382, 420
BseMII CTCAG 1 cut(s) 149
BseSI GKGCMC 1 cut(s) 88
BseXI GCAGC 4 cut(s) 151, 192, 349, 352
Bsh1236I CGCG 1 cut(s) 417
BshFI GGCC 3 cut(s) 12, 214, 358
BsiHKAI GWGCWC 2 cut(s) 88, 156
BslFI GGGAC 1 cut(s) 356
BsmAI GTCTC 2 cut(s) 200, 424
BsmFI GGGAC 1 cut(s) 356
BsnI GGCC 3 cut(s) 12, 214, 358
Bsp1286I GDGCHC 2 cut(s) 88, 156
Bsp143I GATC 2 cut(s) 274, 305
BspACI CCGC 2 cut(s) 60, 351
BspANI GGCC 3 cut(s) 12, 214, 358
BspCNI CTCAG 1 cut(s) 150
BspFNI CGCG 1 cut(s) 417
BspLI GGNNCC 2 cut(s) 344, 345
BssECI CCNNGG 1 cut(s) 293
BssMI GATC 2 cut(s) 274, 305
BssT1I CCWWGG 1 cut(s) 293
Bst2UI CCWGG 1 cut(s) 14
Bst4CI ACNGT 2 cut(s) 47, 269
BstAPI GCANNNNNTGC 1 cut(s) 359
BstBAI YACGTR 1 cut(s) 89
BstC8I GCNNGC 4 cut(s) 60, 301, 360, 450
BstDEI CTNAG 2 cut(s) 70, 158
BstF5I GGATG 3 cut(s) 312, 382, 420
BstFNI CGCG 1 cut(s) 417
BstH2I RGCGCY 1 cut(s) 377
BstHHI GCGC 2 cut(s) 376, 419
BstKTI GATC 2 cut(s) 277, 308
BstMAI GTCTC 2 cut(s) 200, 424
BstMBI GATC 2 cut(s) 274, 305
BstMWI GCNNNNNNNGC 3 cut(s) 189, 359, 371
BstNI CCWGG 1 cut(s) 14
BstSCI CCNGG 1 cut(s) 12
BstSLI GKGCMC 1 cut(s) 88
BstUI CGCG 1 cut(s) 417
BstV1I GCAGC 4 cut(s) 151, 192, 349, 352
BstX2I RGATCY 1 cut(s) 305
BstYI RGATCY 1 cut(s) 305
BsuRI GGCC 3 cut(s) 12, 214, 358
BtgZI GCGATG 1 cut(s) 299
BtsCI GGATG 3 cut(s) 312, 382, 420
Cac8I GCNNGC 4 cut(s) 60, 301, 360, 450
CfoI GCGC 2 cut(s) 376, 419
Cfr13I GGNCC 1 cut(s) 343
CseI GACGC 2 cut(s) 172, 441
CviAII CATG 3 cut(s) 8, 210, 472
DdeI CTNAG 2 cut(s) 70, 158
DpnI GATC 2 cut(s) 276, 307
DpnII GATC 2 cut(s) 274, 305
EaeI YGGCCR 1 cut(s) 212
Ecl136II GAGCTC 1 cut(s) 154
Eco130I CCWWGG 1 cut(s) 293
Eco147I AGGCCT 1 cut(s) 358
Eco24I GRGCYC 1 cut(s) 156
Eco47I GGWCC 1 cut(s) 343
Eco47III AGCGCT 1 cut(s) 375
Eco53kI GAGCTC 1 cut(s) 154
Eco57I CTGAAG 1 cut(s) 390
Eco72I CACGTG 1 cut(s) 89
EcoICRI GAGCTC 1 cut(s) 154
EcoO109I RGGNCCY 1 cut(s) 343
EcoRII CCWGG 1 cut(s) 12
EcoT14I CCWWGG 1 cut(s) 293
EcoT38I GRGCYC 1 cut(s) 156
ErhI CCWWGG 1 cut(s) 293
FaeI CATG 3 cut(s) 11, 213, 475
FaiI YATR 6 cut(s) 9, 21, 23, 211, 249, 473
FalI AAGNNNNNCTT 2 cut(s) 371, 403
FaqI GGGAC 1 cut(s) 356
FatI CATG 3 cut(s) 7, 209, 471
FauI CCCGC 1 cut(s) 67
Fnu4HI GCNGC 4 cut(s) 165, 181, 363, 366
FokI GGATG 3 cut(s) 299, 369, 407
FriOI GRGCYC 1 cut(s) 156
Fsp4HI GCNGC 4 cut(s) 165, 181, 363, 366
GlaI GCGC 2 cut(s) 375, 418
GluI GCNGC 4 cut(s) 165, 181, 363, 366
HaeII RGCGCY 1 cut(s) 377
HaeIII GGCC 3 cut(s) 12, 214, 358
HgaI GACGC 2 cut(s) 172, 441
HhaI GCGC 2 cut(s) 376, 419
Hin1II CATG 3 cut(s) 11, 213, 475
Hin6I GCGC 2 cut(s) 374, 417
HinP1I GCGC 2 cut(s) 374, 417
HincII GTYRAC 1 cut(s) 76
HindII GTYRAC 1 cut(s) 76
HinfI GANTC 3 cut(s) 106, 289, 409
HpaI GTTAAC 1 cut(s) 76
HphI GGTGA 1 cut(s) 126
Hpy166II GTNNAC 2 cut(s) 76, 86
Hpy188III TCNNGA 3 cut(s) 97, 157, 172
Hpy8I GTNNAC 2 cut(s) 76, 86
Hpy99I CGWCG 1 cut(s) 457
HpyAV CCTTC 3 cut(s) 178, 357, 397
HpyCH4III ACNGT 2 cut(s) 47, 269
HpyCH4IV ACGT 3 cut(s) 66, 88, 131
HpyCH4V TGCA 3 cut(s) 25, 86, 180
HpyF10VI GCNNNNNNNGC 3 cut(s) 189, 359, 371
HpyF3I CTNAG 2 cut(s) 70, 158
HpySE526I ACGT 3 cut(s) 66, 88, 131
Hsp92II CATG 3 cut(s) 11, 213, 475
HspAI GCGC 2 cut(s) 374, 417
KflI GGGWCCC 1 cut(s) 343
KspAI GTTAAC 1 cut(s) 76
Kzo9I GATC 2 cut(s) 274, 305
LmnI GCTCC 1 cut(s) 159
LpnPI CCDG 6 cut(s) 26, 170, 228, 329, 372, 462
Lsp1109I GCAGC 4 cut(s) 151, 192, 349, 352
LweI GCATC 1 cut(s) 391
MaeII ACGT 3 cut(s) 66, 88, 131
MaeIII GTNAC 1 cut(s) 132
MalI GATC 2 cut(s) 276, 307
MboI GATC 2 cut(s) 274, 305
MboII GAAGA 2 cut(s) 318, 382
MflI RGATCY 1 cut(s) 305
MhlI GDGCHC 2 cut(s) 88, 156
MlsI TGGCCA 1 cut(s) 214
MluNI TGGCCA 1 cut(s) 214
MmeI TCCRAC 1 cut(s) 221
MnlI CCTC 3 cut(s) 125, 211, 471
Mox20I TGGCCA 1 cut(s) 214
MscI TGGCCA 1 cut(s) 214
MseI TTAA 1 cut(s) 75
Msp20I TGGCCA 1 cut(s) 214
MspA1I CMGCKG 1 cut(s) 319
MspR9I CCNGG 1 cut(s) 14
MvaI CCWGG 1 cut(s) 14
MvnI CGCG 1 cut(s) 417
MwoI GCNNNNNNNGC 3 cut(s) 189, 359, 371
NdeII GATC 2 cut(s) 274, 305
NlaIII CATG 3 cut(s) 11, 213, 475
NlaIV GGNNCC 2 cut(s) 344, 345
NmuCI GTSAC 1 cut(s) 132
PceI AGGCCT 1 cut(s) 358
PcsI WCGNNNNNNNCGW 1 cut(s) 147
PfeI GAWTC 3 cut(s) 106, 289, 409
PflFI GACNNNGTC 1 cut(s) 130
PkrI GCNGC 4 cut(s) 166, 182, 364, 367
PmaCI CACGTG 1 cut(s) 89
PmlI CACGTG 1 cut(s) 89
Ppu21I YACGTR 1 cut(s) 89
PpuMI RGGWCCY 1 cut(s) 343
Psp124BI GAGCTC 1 cut(s) 156
Psp5II RGGWCCY 1 cut(s) 343
Psp6I CCWGG 1 cut(s) 12
PspCI CACGTG 1 cut(s) 89
PspGI CCWGG 1 cut(s) 12
PspN4I GGNNCC 2 cut(s) 344, 345
PspPI GGNCC 1 cut(s) 343
PspPPI RGGWCCY 1 cut(s) 343
PsuI RGATCY 1 cut(s) 305
PsyI GACNNNGTC 1 cut(s) 130
PvuII CAGCTG 1 cut(s) 319
SacI GAGCTC 1 cut(s) 156
SaqAI TTAA 1 cut(s) 75
SatI GCNGC 4 cut(s) 165, 181, 363, 366
Sau3AI GATC 2 cut(s) 274, 305
Sau96I GGNCC 1 cut(s) 343
ScrFI CCNGG 1 cut(s) 14
SduI GDGCHC 2 cut(s) 88, 156
SetI ASST 9 cut(s) 69, 91, 134, 156, 166, 228, 255, 321, 463
SfaNI GCATC 1 cut(s) 391
SinI GGWCC 1 cut(s) 343
SmlI CTYRAG 1 cut(s) 377
SmoI CTYRAG 1 cut(s) 377
SseBI AGGCCT 1 cut(s) 358
SsiI CCGC 2 cut(s) 60, 351
SspI AATATT 1 cut(s) 442
SstI GAGCTC 1 cut(s) 156
StuI AGGCCT 1 cut(s) 358
StyD4I CCNGG 1 cut(s) 12
StyI CCWWGG 1 cut(s) 293
TaaI ACNGT 2 cut(s) 47, 269
TaiI ACGT 3 cut(s) 69, 91, 134
TaqI TCGA 2 cut(s) 150, 455
TfiI GAWTC 3 cut(s) 106, 289, 409
Tru1I TTAA 1 cut(s) 75
Tru9I TTAA 1 cut(s) 75
TseFI GTSAC 1 cut(s) 132
TseI GCWGC 4 cut(s) 164, 180, 362, 365
Tsp45I GTSAC 1 cut(s) 132
TspDTI ATGAA 1 cut(s) 460
Tth111I GACNNNGTC 1 cut(s) 130
VneI GTGCAC 1 cut(s) 84
VpaK11BI GGWCC 1 cut(s) 343
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.