MD04G1061700.v1.1
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000445
Physical Location & Seq
Reverse (-)
8152588 .. 8153553
966 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1061700.v1.1.491

Sequence Viewer

Length: 966 bp
ATGGAAAAGTTCGAGATGATTAAGAAACTAGGTGATGGTGCTTACGGGACGGTGTTTGCTGCGCTCCACAAACAGACGGGTGAACTCGTTGCAATCAAGAAACTGAACCAAAGGTGCAGTTCGTTGCAGCAGTGTCTTAGCCTACCAGAAGTTCAGTCCCTTTCCAAACTCAACCATCCCAACATTGTCCAGTTGAAGGAAGTCATCGCCCAATACAGTTCTGCTTACTTTGTGTTTGAGTATATGCAGAGCAGTTTGAATGATCTTATTGAGACAAAAAGGCAAACCGGATTCACAGAGGACGAAATTAGAAACATCTGCTATCAGATGTTTCAGGGCCTTGCCTCCATGCACAAAACTGGCTATTTTCACCGAGATATGAAGCCGGAGAACGTGTTGGTTAAGGAGGGTGCTGTCAAGATTGGGGATTTAGGTTCTGCTAAGGAGATTGATTCACCTCCTCCTTATACAGACTACGTCACCACTCTCTGGTATCGAGCTCCTGAGGTAATGCTTCGGTCAGACCGCTATTGTCCTAAGGTCGATATGTGGGCAATGGGTGCTATCATGGCAGAGCTTTTCTCGCTCCAGCCTCTATTTCCTGGTCGAAGTGATGCTGATCAAATATTCAAGATTTGCAGCGTGATTGGTAGGCCAACTTTGGGGTCTTGGCGCAACGGGCTGCTTCTTGCACGCCAGATGAACTATCAGTTTCCACAAACTGACGGGTTTGGTCTATCAGCGATGATTCCTACCGCAAGTGAATCAGCCATCAAGCTGATTTCTTCCCTTTGTTCTTGGGACCCTTCAGCGAGGCCTACTGCTGCTGATGCACTCAAGCATCCCTTCTTCGTTGGCAACTACCACGTGCCATCCCTTGGAGACAAAATAATATCCTGCCTGCGTCGAATTCTATTATTTCCGTGTAGCTGTGACACGAGTGATTTGGTAGTTAATTGCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

322

Amino Acids

36.03

Weight (kDa)

7.52

Isoelectric Point (pI)

44.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 4 - 284 4.4e-70 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 5 - 196 1.5e-32 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000445)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G19110 AT4G19110 AT4G19110 AT5G45430 AT5G45430
fragaria_vesca FvH4_2g16890 FvH4_5g19220 FvH4_5g19340 FvH4_5g19350 FvH4_5g19360 FvH4_5g23910 FvH4_7g27810
malus_domestica MD00G1001400.v1.1 MD04G1058900.v1.1 MD04G1059300.v1.1 MD04G1059500.v1.1 MD04G1059600.v1.1 MD04G1059800.v1.1 MD04G1061700.v1.1 MD04G1061800.v1.1 MD06G1014800.v1.1 MD06G1052900.v1.1 MD06G1055500.v1.1
prunus_persica Prupe.5G021300_v2.0.a1 Prupe.5G065900_v2.0.a1 Prupe.5G066500_v2.0.a1
pyrus_communis pycom06g04440
rosa_chinensis RchiOBHm_Chr1g0374681 RchiOBHm_Chr2g0120471 RchiOBHm_Chr2g0120711 RchiOBHm_Chr7g0203821 RchiOBHm_Chr7g0203861 RchiOBHm_Chr7g0208401 RchiOBHm_Chr7g0209011 RchiOBHm_Chr7g0213341
rosa_laevigata RLG00000001348 RLG00000002813 RLG00000003173 RLG00000003212 RLG00000003503 RLG00000022835 RLG00000026723 RLG00000035532
rosa_multiflora Rmu_co7960007.1_g000001 Rmu_co7963796.1_g000001 Rmu_co8519951.1_g000002 Rmu_sc0002038.1_g000009 Rmu_sc0004947.1_g000015 Rmu_sc0007490.1_g000005 Rmu_sc0011180.1_g000003 Rmu_sc0015273.1_g000013 Rmu_ssc0000190.1_g000014 Rmu_ssc0000372.1_g000031
rosa_roxburghii Rroxscaffold_3G00246080 Rroxscaffold_3G00249860 Rroxscaffold_3G00249870 Rroxscaffold_3G00253500 Rroxscaffold_4G00283340
rosa_rugosa Rorug01G0384300 Rorug07G0079000 Rorug07G0079000 Rorug07G0079000 Rorug07G0108300 Rorug07G0141200
rosa_samantha Rh1AG395700 Rh1BG359900 Rh1DG006600 Rh1DG390900 Rh2AG287100 Rh3BG335800 Rh3CG321100 Rh5DG116800 Rh7AG273000 Rh7BG207600 Rh7BG207800 Rh7BG237700 Rh7BG268400 Rh7CG221100 Rh7CG221300 Rh7CG254000 Rh7CG257900 Rh7CG292200 Rh7DG215700 Rh7DG215900 Rh7DG244500 Rh7DG249800 Rh7DG280700
rosa_wichuraiana Rw0G010370 Rw1G035090 Rw2G023020 Rw7G018200 Rw7G020570 Rw7G023690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 489, 878
AciI CCGC 2 cut(s) 526, 756
AcsI RAATTY 1 cut(s) 909
AcuI CTGAAG 1 cut(s) 792
AcvI CACGTG 1 cut(s) 868
AfiI CCNNNNNNNGG 4 cut(s) 196, 489, 662, 878
AflIII ACRYGT 1 cut(s) 393
AgsI TTSAA 3 cut(s) 196, 259, 631
AjnI CCWGG 1 cut(s) 601
AluBI AGCT 4 cut(s) 500, 577, 778, 930
AluI AGCT 4 cut(s) 500, 577, 778, 930
Alw21I GWGCWC 1 cut(s) 502
Alw26I GTCTC 2 cut(s) 266, 876
AoxI GGCC 3 cut(s) 337, 653, 815
ApeKI GCWGC 5 cut(s) 59, 127, 639, 682, 824
ApoI RAATTY 1 cut(s) 909
AspLEI GCGC 2 cut(s) 64, 675
AspS9I GGNCC 2 cut(s) 337, 802
AsuHPI GGTGA 5 cut(s) 44, 92, 362, 447, 472
AvaII GGWCC 1 cut(s) 802
AxyI CCTNAGG 2 cut(s) 504, 537
BanII GRGCYC 1 cut(s) 502
BauI CACGAG 1 cut(s) 937
BbrPI CACGTG 1 cut(s) 868
Bbv12I GWGCWC 1 cut(s) 502
BbvI GCAGC 5 cut(s) 46, 139, 651, 669, 811
BccI CCATC 4 cut(s) 29, 183, 779, 880
BciT130I CCWGG 1 cut(s) 603
BclI TGATCA 1 cut(s) 619
BcoDI GTCTC 2 cut(s) 266, 876
BfaI CTAG 1 cut(s) 29
BisI GCNGC 5 cut(s) 60, 128, 640, 683, 825
BlsI GCNGC 5 cut(s) 61, 129, 641, 684, 826
Bme1390I CCNGG 1 cut(s) 603
Bme18I GGWCC 1 cut(s) 802
BmgT120I GGNCC 2 cut(s) 337, 802
BmiI GGNNCC 2 cut(s) 803, 804
BmrFI CCNGG 1 cut(s) 603
BmsI GCATC 3 cut(s) 604, 820, 850
BplI GAGNNNNNCTC 2 cut(s) 566, 598
BpmI CTGGAG 1 cut(s) 572
Bpu10I CCTNAGC 1 cut(s) 441
BpuEI CTTGAG 1 cut(s) 821
BsaAI YACGTR 1 cut(s) 868
BsaBI GATNNNNATC 1 cut(s) 618
BsaJI CCNNGG 1 cut(s) 877
BsaWI WCCGGW 1 cut(s) 287
BsaXI ACNNNNNCTCC 2 cut(s) 380, 410
Bsc4I CCNNNNNNNGG 4 cut(s) 196, 489, 662, 878
Bse1I ACTGG 2 cut(s) 190, 364
Bse21I CCTNAGG 2 cut(s) 504, 537
Bse3DI GCAATG 1 cut(s) 561
Bse8I GATNNNNATC 1 cut(s) 618
BseBI CCWGG 1 cut(s) 603
BseDI CCNNGG 1 cut(s) 877
BseGI GGATG 3 cut(s) 175, 841, 872
BseJI GATNNNNATC 1 cut(s) 618
BseLI CCNNNNNNNGG 4 cut(s) 196, 489, 662, 878
BseMI GCAATG 1 cut(s) 561
BseMII CTCAG 1 cut(s) 495
BseNI ACTGG 2 cut(s) 190, 364
BseRI GAGGAG 1 cut(s) 450
BseXI GCAGC 5 cut(s) 46, 139, 651, 669, 811
BsgI GTGCAG 1 cut(s) 136
BshFI GGCC 3 cut(s) 339, 655, 817
BsiHKAI GWGCWC 1 cut(s) 502
BsiSI CCGG 2 cut(s) 288, 386
BslFI GGGAC 3 cut(s) 61, 142, 815
BslI CCNNNNNNNGG 4 cut(s) 196, 489, 662, 878
BsmAI GTCTC 2 cut(s) 266, 876
BsmFI GGGAC 3 cut(s) 61, 142, 815
BsnI GGCC 3 cut(s) 339, 655, 817
Bsp1286I GDGCHC 1 cut(s) 502
Bsp143I GATC 2 cut(s) 262, 619
BspACI CCGC 2 cut(s) 526, 756
BspANI GGCC 3 cut(s) 339, 655, 817
BspCNI CTCAG 1 cut(s) 496
BspLI GGNNCC 2 cut(s) 803, 804
BsrDI GCAATG 1 cut(s) 561
BsrI ACTGG 2 cut(s) 190, 364
BssECI CCNNGG 1 cut(s) 877
BssMI GATC 2 cut(s) 262, 619
BssSI CACGAG 1 cut(s) 937
BssT1I CCWWGG 1 cut(s) 877
Bst2BI CACGAG 1 cut(s) 937
Bst2UI CCWGG 1 cut(s) 603
Bst4CI ACNGT 2 cut(s) 52, 218
BstAPI GCANNNNNTGC 1 cut(s) 560
BstBAI YACGTR 1 cut(s) 868
BstC8I GCNNGC 2 cut(s) 694, 902
BstDEI CTNAG 4 cut(s) 137, 441, 504, 537
BstF5I GGATG 3 cut(s) 175, 841, 872
BstHHI GCGC 2 cut(s) 64, 675
BstKTI GATC 2 cut(s) 265, 622
BstMAI GTCTC 2 cut(s) 266, 876
BstMBI GATC 2 cut(s) 262, 619
BstMWI GCNNNNNNNGC 5 cut(s) 560, 569, 583, 679, 830
BstNI CCWGG 1 cut(s) 603
BstSCI CCNGG 1 cut(s) 601
BstV1I GCAGC 5 cut(s) 46, 139, 651, 669, 811
Bsu36I CCTNAGG 2 cut(s) 504, 537
BsuRI GGCC 3 cut(s) 339, 655, 817
BtgZI GCGATG 2 cut(s) 190, 758
BtsCI GGATG 3 cut(s) 175, 841, 872
BtsI GCAGTG 1 cut(s) 137
BtsIMutI CAGTG 1 cut(s) 137
Cac8I GCNNGC 2 cut(s) 694, 902
CfoI GCGC 2 cut(s) 64, 675
Cfr13I GGNCC 2 cut(s) 337, 802
CseI GACGC 1 cut(s) 893
CviAII CATG 2 cut(s) 349, 568
DdeI CTNAG 4 cut(s) 137, 441, 504, 537
DpnI GATC 2 cut(s) 264, 621
DpnII GATC 2 cut(s) 262, 619
Ecl136II GAGCTC 1 cut(s) 500
Eco130I CCWWGG 1 cut(s) 877
Eco147I AGGCCT 1 cut(s) 817
Eco24I GRGCYC 1 cut(s) 502
Eco47I GGWCC 1 cut(s) 802
Eco53kI GAGCTC 1 cut(s) 500
Eco57I CTGAAG 1 cut(s) 792
Eco72I CACGTG 1 cut(s) 868
Eco81I CCTNAGG 2 cut(s) 504, 537
EcoICRI GAGCTC 1 cut(s) 500
EcoO109I RGGNCCY 2 cut(s) 337, 802
EcoRI GAATTC 1 cut(s) 909
EcoRII CCWGG 1 cut(s) 601
EcoT14I CCWWGG 1 cut(s) 877
EcoT38I GRGCYC 1 cut(s) 502
ErhI CCWWGG 1 cut(s) 877
FaeI CATG 2 cut(s) 352, 571
FaiI YATR 7 cut(s) 243, 245, 350, 380, 468, 548, 569
FalI AAGNNNNNCTT 2 cut(s) 830, 862
FaqI GGGAC 3 cut(s) 61, 142, 815
FatI CATG 2 cut(s) 348, 567
FbaI TGATCA 1 cut(s) 619
Fnu4HI GCNGC 5 cut(s) 60, 128, 640, 683, 825
FokI GGATG 3 cut(s) 162, 828, 859
FriOI GRGCYC 1 cut(s) 502
Fsp4HI GCNGC 5 cut(s) 60, 128, 640, 683, 825
FspBI CTAG 1 cut(s) 29
GlaI GCGC 2 cut(s) 63, 674
GluI GCNGC 5 cut(s) 60, 128, 640, 683, 825
GsuI CTGGAG 1 cut(s) 572
HaeIII GGCC 3 cut(s) 339, 655, 817
HapII CCGG 2 cut(s) 288, 386
HgaI GACGC 1 cut(s) 893
HhaI GCGC 2 cut(s) 64, 675
Hin1II CATG 2 cut(s) 352, 571
Hin6I GCGC 2 cut(s) 62, 673
HinP1I GCGC 2 cut(s) 62, 673
HinfI GANTC 4 cut(s) 291, 452, 748, 764
HpaII CCGG 2 cut(s) 288, 386
HphI GGTGA 5 cut(s) 44, 92, 362, 447, 472
Hpy166II GTNNAC 1 cut(s) 83
Hpy188I TCNGA 2 cut(s) 327, 523
Hpy188III TCNNGA 5 cut(s) 13, 97, 418, 503, 631
Hpy8I GTNNAC 1 cut(s) 83
Hpy99I CGWCG 1 cut(s) 909
HpyAV CCTTC 3 cut(s) 190, 816, 856
HpyCH4III ACNGT 2 cut(s) 52, 218
HpyCH4IV ACGT 3 cut(s) 393, 477, 867
HpyCH4V TGCA 8 cut(s) 92, 117, 127, 247, 352, 639, 692, 833
HpyF10VI GCNNNNNNNGC 5 cut(s) 560, 569, 583, 679, 830
HpyF3I CTNAG 4 cut(s) 137, 441, 504, 537
HpySE526I ACGT 3 cut(s) 393, 477, 867
Hsp92II CATG 2 cut(s) 352, 571
HspAI GCGC 2 cut(s) 62, 673
KflI GGGWCCC 1 cut(s) 802
Ksp22I TGATCA 1 cut(s) 619
Kzo9I GATC 2 cut(s) 262, 619
LmnI GCTCC 3 cut(s) 69, 505, 591
Lsp1109I GCAGC 5 cut(s) 46, 139, 651, 669, 811
LweI GCATC 3 cut(s) 604, 820, 850
MaeI CTAG 1 cut(s) 29
MaeII ACGT 3 cut(s) 393, 477, 867
MaeIII GTNAC 2 cut(s) 478, 932
MalI GATC 2 cut(s) 264, 621
MboI GATC 2 cut(s) 262, 619
MboII GAAGA 2 cut(s) 777, 841
MhlI GDGCHC 1 cut(s) 502
MluCI AATT 3 cut(s) 306, 909, 955
MnlI CCTC 8 cut(s) 292, 355, 400, 468, 471, 499, 603, 807
MseI TTAA 3 cut(s) 21, 402, 954
MspI CCGG 2 cut(s) 288, 386
MspR9I CCNGG 1 cut(s) 603
MvaI CCWGG 1 cut(s) 603
MwoI GCNNNNNNNGC 5 cut(s) 560, 569, 583, 679, 830
NdeII GATC 2 cut(s) 262, 619
NlaIII CATG 2 cut(s) 352, 571
NlaIV GGNNCC 2 cut(s) 803, 804
NmuCI GTSAC 2 cut(s) 478, 932
PceI AGGCCT 1 cut(s) 817
PfeI GAWTC 4 cut(s) 291, 452, 748, 764
PflFI GACNNNGTC 1 cut(s) 476
PflMI CCANNNNNTGG 2 cut(s) 489, 878
PkrI GCNGC 5 cut(s) 61, 129, 641, 684, 826
PmaCI CACGTG 1 cut(s) 868
PmlI CACGTG 1 cut(s) 868
Ppu21I YACGTR 1 cut(s) 868
PpuMI RGGWCCY 1 cut(s) 802
Psp124BI GAGCTC 1 cut(s) 502
Psp5II RGGWCCY 1 cut(s) 802
Psp6I CCWGG 1 cut(s) 601
PspCI CACGTG 1 cut(s) 868
PspGI CCWGG 1 cut(s) 601
PspN4I GGNNCC 2 cut(s) 803, 804
PspPI GGNCC 2 cut(s) 337, 802
PspPPI RGGWCCY 1 cut(s) 802
PsyI GACNNNGTC 1 cut(s) 476
SacI GAGCTC 1 cut(s) 502
SaqAI TTAA 3 cut(s) 21, 402, 954
SatI GCNGC 5 cut(s) 60, 128, 640, 683, 825
Sau3AI GATC 2 cut(s) 262, 619
Sau96I GGNCC 2 cut(s) 337, 802
ScrFI CCNGG 1 cut(s) 603
SduI GDGCHC 1 cut(s) 502
SfaNI GCATC 3 cut(s) 604, 820, 850
SinI GGWCC 1 cut(s) 802
SmlI CTYRAG 1 cut(s) 836
SmoI CTYRAG 1 cut(s) 836
Sse9I AATT 3 cut(s) 306, 909, 955
SseBI AGGCCT 1 cut(s) 817
SsiI CCGC 2 cut(s) 526, 756
SspI AATATT 1 cut(s) 627
SspMI CTAG 1 cut(s) 29
SstI GAGCTC 1 cut(s) 502
StuI AGGCCT 1 cut(s) 817
StyD4I CCNGG 1 cut(s) 601
StyI CCWWGG 1 cut(s) 877
TaaI ACNGT 2 cut(s) 52, 218
TaiI ACGT 3 cut(s) 396, 480, 870
TaqI TCGA 5 cut(s) 12, 496, 543, 607, 907
TaqII GACCGA 1 cut(s) 507
TasI AATT 3 cut(s) 306, 909, 955
TfiI GAWTC 4 cut(s) 291, 452, 748, 764
Tru1I TTAA 3 cut(s) 21, 402, 954
Tru9I TTAA 3 cut(s) 21, 402, 954
TscAI CASTG 1 cut(s) 137
TseFI GTSAC 2 cut(s) 478, 932
TseI GCWGC 5 cut(s) 59, 127, 639, 682, 824
Tsp45I GTSAC 2 cut(s) 478, 932
TspDTI ATGAA 2 cut(s) 395, 716
TspGWI ACGGA 1 cut(s) 912
TspRI CASTG 1 cut(s) 137
Tth111I GACNNNGTC 1 cut(s) 476
Van91I CCANNNNNTGG 2 cut(s) 489, 878
VpaK11BI GGWCC 1 cut(s) 802
XapI RAATTY 1 cut(s) 909
XspI CTAG 1 cut(s) 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.