pycom06g04440
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0000445
Physical Location & Seq
Forward (+)
5693291 .. 5694236
946 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g04440.2

Sequence Viewer

Length: 735 bp
ATGGGAGAGATCAGTTCCTTTGAGCAGTGTCTAACACTACCGGAAGTTCAGTCCCTTTCCAAGCTCAAGCATCCCAGCATTGTCCAGTTGAAGAGTATCACGTCACCACTCGCCCTTATCGAGCTCCTGAGGTATTGCTTCGGTCAGGCTTCTAGGGCCCAAAGGTTGATATGTGGGCAAGGGCGCCATCATGGCAGAGCTGCTTTCAAGATTTGCAGCGTCATTGGCAGTCCAACTTGGGAGACATGGCCAGAGGGAGAGCTTCTTGCCCAAAACTTGAACTATGAGTTTCCACAACTTCGCGGTGTTGGTCTATCGGCGATGATTCCTTCGGCAAGCAGATCCGCCATCCAGCTGATTTCTTCATTTCGTTCTTGGGACCCTTCCGCAAGGCCCGCTGCTGCTGAAGCGCTCAAGCCAAGCATCCCTTCTTCGTTGGCAACCACAGGATTCCACGCACCATCCCTTCCAGACAACGCAATGCATCGAAATCTCTTATTTTCGTGTAGATTTGACACGACTAATGTTGTAGTTAATTGCTTTCAATTCATTAGCCTTCAAAAGCTACAAATTTCATTTAACGAGTCCCGTTGTTTTGTTTGGTTCAAATTTCATTATTCCAAACATATGCCAAATCAAATATCGACGACAAATTACTTGCAGGCTAATTTGAGAGAAAAGAAACTCAAAAACATAACACGAGGCTTTAATTTACAACTCAATCCGTACACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

245

Amino Acids

27.43

Weight (kDa)

9.58

Isoelectric Point (pI)

45.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000445)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G19110 AT4G19110 AT4G19110 AT5G45430 AT5G45430
fragaria_vesca FvH4_2g16890 FvH4_5g19220 FvH4_5g19340 FvH4_5g19350 FvH4_5g19360 FvH4_5g23910 FvH4_7g27810
malus_domestica MD00G1001400.v1.1 MD04G1058900.v1.1 MD04G1059300.v1.1 MD04G1059500.v1.1 MD04G1059600.v1.1 MD04G1059800.v1.1 MD04G1061700.v1.1 MD04G1061800.v1.1 MD06G1014800.v1.1 MD06G1052900.v1.1 MD06G1055500.v1.1
prunus_persica Prupe.5G021300_v2.0.a1 Prupe.5G065900_v2.0.a1 Prupe.5G066500_v2.0.a1
pyrus_communis pycom06g04440
rosa_chinensis RchiOBHm_Chr1g0374681 RchiOBHm_Chr2g0120471 RchiOBHm_Chr2g0120711 RchiOBHm_Chr7g0203821 RchiOBHm_Chr7g0203861 RchiOBHm_Chr7g0208401 RchiOBHm_Chr7g0209011 RchiOBHm_Chr7g0213341
rosa_laevigata RLG00000001348 RLG00000002813 RLG00000003173 RLG00000003212 RLG00000003503 RLG00000022835 RLG00000026723 RLG00000035532
rosa_multiflora Rmu_co7960007.1_g000001 Rmu_co7963796.1_g000001 Rmu_co8519951.1_g000002 Rmu_sc0002038.1_g000009 Rmu_sc0004947.1_g000015 Rmu_sc0007490.1_g000005 Rmu_sc0011180.1_g000003 Rmu_sc0015273.1_g000013 Rmu_ssc0000190.1_g000014 Rmu_ssc0000372.1_g000031
rosa_roxburghii Rroxscaffold_3G00246080 Rroxscaffold_3G00249860 Rroxscaffold_3G00249870 Rroxscaffold_3G00253500 Rroxscaffold_4G00283340
rosa_rugosa Rorug01G0384300 Rorug07G0079000 Rorug07G0079000 Rorug07G0079000 Rorug07G0108300 Rorug07G0141200
rosa_samantha Rh1AG395700 Rh1BG359900 Rh1DG006600 Rh1DG390900 Rh2AG287100 Rh3BG335800 Rh3CG321100 Rh5DG116800 Rh7AG273000 Rh7BG207600 Rh7BG207800 Rh7BG237700 Rh7BG268400 Rh7CG221100 Rh7CG221300 Rh7CG254000 Rh7CG257900 Rh7CG292200 Rh7DG215700 Rh7DG215900 Rh7DG244500 Rh7DG249800 Rh7DG280700
rosa_wichuraiana Rw0G010370 Rw1G035090 Rw2G023020 Rw7G018200 Rw7G020570 Rw7G023690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 183
AccII CGCG 1 cut(s) 303
AciI CCGC 4 cut(s) 303, 345, 387, 396
AclWI GGATC 1 cut(s) 336
AcoI YGGCCR 1 cut(s) 248
AcsI RAATTY 2 cut(s) 570, 608
AcuI CTGAAG 1 cut(s) 426
AcyI GRCGYC 1 cut(s) 184
AfaI GTAC 1 cut(s) 728
AfeI AGCGCT 1 cut(s) 411
AgsI TTSAA 6 cut(s) 91, 208, 280, 545, 560, 607
AjiI CACGTC 1 cut(s) 102
AjuI GAANNNNNNNTTGG 2 cut(s) 412, 444
AluBI AGCT 6 cut(s) 64, 124, 200, 262, 355, 565
AluI AGCT 6 cut(s) 64, 124, 200, 262, 355, 565
Alw21I GWGCWC 1 cut(s) 126
Alw26I GTCTC 1 cut(s) 236
AlwI GGATC 1 cut(s) 336
Aor51HI AGCGCT 1 cut(s) 411
AoxI GGCC 3 cut(s) 156, 248, 392
ApaI GGGCCC 1 cut(s) 160
ApeKI GCWGC 4 cut(s) 200, 216, 398, 401
ApoI RAATTY 2 cut(s) 570, 608
AspLEI GCGC 2 cut(s) 186, 412
AspS9I GGNCC 4 cut(s) 156, 157, 379, 393
AsuHPI GGTGA 1 cut(s) 96
AvaII GGWCC 1 cut(s) 379
AxyI CCTNAGG 1 cut(s) 128
BaeGI GKGCMC 1 cut(s) 160
BalI TGGCCA 1 cut(s) 250
BanI GGYRCC 1 cut(s) 183
BanII GRGCYC 2 cut(s) 126, 160
BauI CACGAG 1 cut(s) 699
Bbv12I GWGCWC 1 cut(s) 126
BbvI GCAGC 4 cut(s) 187, 228, 385, 388
BccI CCATC 3 cut(s) 195, 356, 469
BcoDI GTCTC 1 cut(s) 236
BfaI CTAG 1 cut(s) 153
BfoI RGCGCY 2 cut(s) 187, 413
BglI GCCNNNNNGGC 1 cut(s) 192
BisI GCNGC 4 cut(s) 201, 217, 399, 402
BlsI GCNGC 4 cut(s) 202, 218, 400, 403
Bme18I GGWCC 1 cut(s) 379
BmgBI CACGTC 1 cut(s) 102
BmgT120I GGNCC 4 cut(s) 156, 157, 379, 393
BmiI GGNNCC 4 cut(s) 158, 185, 380, 381
BmsI GCATC 3 cut(s) 79, 432, 493
BpuEI CTTGAG 2 cut(s) 50, 398
BsaHI GRCGYC 1 cut(s) 184
BsaWI WCCGGW 1 cut(s) 40
Bse1I ACTGG 1 cut(s) 85
Bse21I CCTNAGG 1 cut(s) 128
Bse3DI GCAATG 1 cut(s) 486
BseGI GGATG 4 cut(s) 70, 348, 423, 461
BseMI GCAATG 1 cut(s) 486
BseMII CTCAG 1 cut(s) 119
BseNI ACTGG 1 cut(s) 85
BseSI GKGCMC 1 cut(s) 160
BseXI GCAGC 4 cut(s) 187, 228, 385, 388
BseYI CCCAGC 1 cut(s) 74
Bsh1236I CGCG 1 cut(s) 303
BshFI GGCC 3 cut(s) 158, 250, 394
BshNI GGYRCC 1 cut(s) 183
BsiHKAI GWGCWC 1 cut(s) 126
BsiSI CCGG 1 cut(s) 41
BslFI GGGAC 3 cut(s) 37, 392, 571
BsmAI GTCTC 1 cut(s) 236
BsmFI GGGAC 3 cut(s) 37, 392, 571
BsnI GGCC 3 cut(s) 158, 250, 394
Bsp120I GGGCCC 1 cut(s) 156
Bsp1286I GDGCHC 2 cut(s) 126, 160
Bsp143I GATC 2 cut(s) 9, 341
BspACI CCGC 4 cut(s) 303, 345, 387, 396
BspANI GGCC 3 cut(s) 158, 250, 394
BspCNI CTCAG 1 cut(s) 120
BspFNI CGCG 1 cut(s) 303
BspLI GGNNCC 4 cut(s) 158, 185, 380, 381
BspPI GGATC 1 cut(s) 336
BspT107I GGYRCC 1 cut(s) 183
BsrDI GCAATG 1 cut(s) 486
BsrI ACTGG 1 cut(s) 85
BssMI GATC 2 cut(s) 9, 341
BssNI GRCGYC 1 cut(s) 184
BssSI CACGAG 1 cut(s) 699
Bst2BI CACGAG 1 cut(s) 699
Bst6I CTCTTC 1 cut(s) 86
BstACI GRCGYC 1 cut(s) 184
BstC8I GCNNGC 3 cut(s) 337, 396, 663
BstDEI CTNAG 1 cut(s) 128
BstF5I GGATG 4 cut(s) 70, 348, 423, 461
BstFNI CGCG 1 cut(s) 303
BstH2I RGCGCY 2 cut(s) 187, 413
BstHHI GCGC 2 cut(s) 186, 412
BstKTI GATC 2 cut(s) 12, 344
BstMAI GTCTC 1 cut(s) 236
BstMBI GATC 2 cut(s) 9, 341
BstMWI GCNNNNNNNGC 5 cut(s) 155, 192, 225, 395, 407
BstSLI GKGCMC 1 cut(s) 160
BstUI CGCG 1 cut(s) 303
BstV1I GCAGC 4 cut(s) 187, 228, 385, 388
BstX2I RGATCY 1 cut(s) 341
BstYI RGATCY 1 cut(s) 341
Bsu36I CCTNAGG 1 cut(s) 128
BsuRI GGCC 3 cut(s) 158, 250, 394
BtgZI GCGATG 1 cut(s) 335
BtrI CACGTC 1 cut(s) 102
BtsCI GGATG 4 cut(s) 70, 348, 423, 461
BtsI GCAGTG 1 cut(s) 32
BtsIMutI CAGTG 1 cut(s) 32
Cac8I GCNNGC 3 cut(s) 337, 396, 663
CfoI GCGC 2 cut(s) 186, 412
Cfr13I GGNCC 4 cut(s) 156, 157, 379, 393
CseI GACGC 1 cut(s) 208
Csp6I GTAC 1 cut(s) 727
CviAII CATG 3 cut(s) 191, 246, 732
CviQI GTAC 1 cut(s) 727
DdeI CTNAG 1 cut(s) 128
DinI GGCGCC 1 cut(s) 185
DpnI GATC 2 cut(s) 11, 343
DpnII GATC 2 cut(s) 9, 341
EaeI YGGCCR 1 cut(s) 248
Eam1104I CTCTTC 1 cut(s) 86
EarI CTCTTC 1 cut(s) 86
EciI GGCGGA 1 cut(s) 334
Ecl136II GAGCTC 1 cut(s) 124
Eco24I GRGCYC 2 cut(s) 126, 160
Eco47I GGWCC 1 cut(s) 379
Eco47III AGCGCT 1 cut(s) 411
Eco53kI GAGCTC 1 cut(s) 124
Eco57I CTGAAG 1 cut(s) 426
Eco81I CCTNAGG 1 cut(s) 128
EcoICRI GAGCTC 1 cut(s) 124
EcoO109I RGGNCCY 2 cut(s) 156, 379
EcoT22I ATGCAT 1 cut(s) 486
EcoT38I GRGCYC 2 cut(s) 126, 160
EgeI GGCGCC 1 cut(s) 185
EheI GGCGCC 1 cut(s) 185
FaeI CATG 3 cut(s) 194, 249, 735
FaiI YATR 8 cut(s) 172, 192, 247, 285, 627, 629, 695, 733
FalI AAGNNNNNCTT 2 cut(s) 412, 444
FaqI GGGAC 3 cut(s) 37, 392, 571
FatI CATG 3 cut(s) 190, 245, 731
FauI CCCGC 1 cut(s) 403
FauNDI CATATG 1 cut(s) 627
Fnu4HI GCNGC 4 cut(s) 201, 217, 399, 402
FokI GGATG 4 cut(s) 57, 335, 410, 448
FriOI GRGCYC 2 cut(s) 126, 160
Fsp4HI GCNGC 4 cut(s) 201, 217, 399, 402
FspBI CTAG 1 cut(s) 153
GlaI GCGC 2 cut(s) 185, 411
GluI GCNGC 4 cut(s) 201, 217, 399, 402
GsaI CCCAGC 1 cut(s) 78
HaeII RGCGCY 2 cut(s) 187, 413
HaeIII GGCC 3 cut(s) 158, 250, 394
HapII CCGG 1 cut(s) 41
HgaI GACGC 1 cut(s) 208
HhaI GCGC 2 cut(s) 186, 412
Hin1I GRCGYC 1 cut(s) 184
Hin1II CATG 3 cut(s) 194, 249, 735
Hin6I GCGC 2 cut(s) 184, 410
HinP1I GCGC 2 cut(s) 184, 410
HinfI GANTC 3 cut(s) 325, 450, 584
HpaII CCGG 1 cut(s) 41
HphI GGTGA 1 cut(s) 96
Hpy166II GTNNAC 1 cut(s) 729
Hpy188III TCNNGA 3 cut(s) 127, 208, 470
Hpy8I GTNNAC 1 cut(s) 729
Hpy99I CGWCG 1 cut(s) 649
HpyAV CCTTC 5 cut(s) 339, 393, 438, 476, 566
HpyCH4IV ACGT 1 cut(s) 101
HpyCH4V TGCA 3 cut(s) 216, 484, 661
HpyF10VI GCNNNNNNNGC 5 cut(s) 155, 192, 225, 395, 407
HpyF3I CTNAG 1 cut(s) 128
HpySE526I ACGT 1 cut(s) 101
Hsp92I GRCGYC 1 cut(s) 184
Hsp92II CATG 3 cut(s) 194, 249, 735
HspAI GCGC 2 cut(s) 184, 410
KasI GGCGCC 1 cut(s) 183
KflI GGGWCCC 1 cut(s) 379
Kzo9I GATC 2 cut(s) 9, 341
LmnI GCTCC 1 cut(s) 129
Lsp1109I GCAGC 4 cut(s) 187, 228, 385, 388
LweI GCATC 3 cut(s) 79, 432, 493
MaeI CTAG 1 cut(s) 153
MaeII ACGT 1 cut(s) 101
MaeIII GTNAC 1 cut(s) 102
MalI GATC 2 cut(s) 11, 343
MboI GATC 2 cut(s) 9, 341
MboII GAAGA 3 cut(s) 103, 354, 423
MflI RGATCY 1 cut(s) 341
MhlI GDGCHC 2 cut(s) 126, 160
MlsI TGGCCA 1 cut(s) 250
MluCI AATT 7 cut(s) 535, 545, 570, 608, 652, 667, 709
MluNI TGGCCA 1 cut(s) 250
Mly113I GGCGCC 1 cut(s) 184
MlyI GAGTC 1 cut(s) 593
MmeI TCCRAC 1 cut(s) 257
MnlI CCTC 3 cut(s) 123, 247, 695
Mox20I TGGCCA 1 cut(s) 250
Mph1103I ATGCAT 1 cut(s) 486
MscI TGGCCA 1 cut(s) 250
MseI TTAA 3 cut(s) 534, 579, 708
Msp20I TGGCCA 1 cut(s) 250
MspA1I CMGCKG 2 cut(s) 355, 398
MspI CCGG 1 cut(s) 41
MvnI CGCG 1 cut(s) 303
MwoI GCNNNNNNNGC 5 cut(s) 155, 192, 225, 395, 407
NarI GGCGCC 1 cut(s) 184
NdeI CATATG 1 cut(s) 627
NdeII GATC 2 cut(s) 9, 341
NlaIII CATG 3 cut(s) 194, 249, 735
NlaIV GGNNCC 4 cut(s) 158, 185, 380, 381
NmuCI GTSAC 1 cut(s) 102
NsiI ATGCAT 1 cut(s) 486
PcsI WCGNNNNNNNCGW 1 cut(s) 117
PfeI GAWTC 2 cut(s) 325, 450
PkrI GCNGC 4 cut(s) 202, 218, 400, 403
PleI GAGTC 1 cut(s) 592
PluTI GGCGCC 1 cut(s) 187
PpsI GAGTC 1 cut(s) 592
PpuMI RGGWCCY 1 cut(s) 379
Psp124BI GAGCTC 1 cut(s) 126
Psp5II RGGWCCY 1 cut(s) 379
PspFI CCCAGC 1 cut(s) 74
PspN4I GGNNCC 4 cut(s) 158, 185, 380, 381
PspOMI GGGCCC 1 cut(s) 156
PspPI GGNCC 4 cut(s) 156, 157, 379, 393
PspPPI RGGWCCY 1 cut(s) 379
PsuI RGATCY 1 cut(s) 341
PvuII CAGCTG 1 cut(s) 355
RsaI GTAC 1 cut(s) 728
RsaNI GTAC 1 cut(s) 727
SacI GAGCTC 1 cut(s) 126
SaqAI TTAA 3 cut(s) 534, 579, 708
SatI GCNGC 4 cut(s) 201, 217, 399, 402
Sau3AI GATC 2 cut(s) 9, 341
Sau96I GGNCC 4 cut(s) 156, 157, 379, 393
SchI GAGTC 1 cut(s) 593
SduI GDGCHC 2 cut(s) 126, 160
SetI ASST 9 cut(s) 66, 104, 126, 134, 167, 202, 264, 357, 567
SfaNI GCATC 3 cut(s) 79, 432, 493
SfoI GGCGCC 1 cut(s) 185
SinI GGWCC 1 cut(s) 379
SmlI CTYRAG 2 cut(s) 65, 413
SmoI CTYRAG 2 cut(s) 65, 413
Sse9I AATT 7 cut(s) 535, 545, 570, 608, 652, 667, 709
SsiI CCGC 4 cut(s) 303, 345, 387, 396
SspDI GGCGCC 1 cut(s) 183
SspMI CTAG 1 cut(s) 153
SstI GAGCTC 1 cut(s) 126
TaiI ACGT 1 cut(s) 104
TaqI TCGA 3 cut(s) 120, 487, 644
TaqII GACCGA 1 cut(s) 131
TasI AATT 7 cut(s) 535, 545, 570, 608, 652, 667, 709
TfiI GAWTC 2 cut(s) 325, 450
Tru1I TTAA 3 cut(s) 534, 579, 708
Tru9I TTAA 3 cut(s) 534, 579, 708
TscAI CASTG 1 cut(s) 32
TseFI GTSAC 1 cut(s) 102
TseI GCWGC 4 cut(s) 200, 216, 398, 401
Tsp45I GTSAC 1 cut(s) 102
TspDTI ATGAA 4 cut(s) 354, 538, 564, 602
TspGWI ACGGA 1 cut(s) 714
TspRI CASTG 1 cut(s) 32
VpaK11BI GGWCC 1 cut(s) 379
XapI RAATTY 2 cut(s) 570, 608
XspI CTAG 1 cut(s) 153
Zsp2I ATGCAT 1 cut(s) 486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.