MD04G1059300.v1.1
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000445
Physical Location & Seq
Forward (+)
7728511 .. 7729449
939 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1059300.v1.1.491

Sequence Viewer

Length: 939 bp
ATGGACAAGTTTTGGATTACTAAGCAACTAGGCGGTGGTTCTTTCGGAACGGTATTTGAGGCGCAGCACCGACCGACGGGTGAAGTCGTTGCAGTCAAGTATCTGAGGGAGACATTCAGTTCCTTTGAGCAGTGCTTAAGCCTACCGGAAGTTCAGTCCCTTTCCAAGCTCAGGCACCCCAACATTGTCCAGTTGAAGGATGTCATCTTCGAAAACGATTCTGCATTCCTTGTCTTTGAGTATATGCAGAGCAGTCTGCTTGATCTTATGATGAAAAGGCGAACCAAATTCACCGAGGAAGAAGTTAGAAGCATCTGCTTTCAGAAGTTTCAGGGCCTGGCTTATATGCACAGAAACGGCTATTTCCACCGTGATTTGAAGCCCGCTAATGTGTTGGTTAAGGATGGTGCGCGTGTGGTCAAGATTGCAGATTTGGGTTCGGCTAAGGAGATTGATTCACCTCCTCCCTATACAGACTACGTCACTACTCGTCCGTATCGTGCTCCTGAGGTGTTTCTTCGGTCAGGCTTGTATGGTCCTAAGGTCGATATGTGGGCAATGGTTGCCATCATAGCGGAGCTGTTTTCATTCTGGCCTCTATTTCCCGGTGAAAGTGCTGATGATCAGATGTTCAAGATTTGCAGTGTTATAGGCAGTCCCACTTGGGAGTCATGGCCAGAGGGACAGCTTCTTGCCCAAAACTTGAACTATCAGTTTCCAAAAATTGGCGGGGTTGGTCTATCTGCGATGATTCCTTCTGCAAGCAGATCTGCCATCCAGCTGATTTCTTCCCTTTGTTCTTGGGACCCTTCCGCGAGGCCTACTGCTGCTGAGGCACTACGGCATCCTTTCTTCGTTGGCAACTACAAGATTCCACGAGCCATCCCTTTGAGACAAAGCAATATCCTGCCTGCATCAAACTCTCTTATTTTCGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

313

Amino Acids

35.16

Weight (kDa)

8.57

Isoelectric Point (pI)

48.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 6 - 197 5.4e-33 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 7 - 285 9.2e-65 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000445)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G19110 AT4G19110 AT4G19110 AT5G45430 AT5G45430
fragaria_vesca FvH4_2g16890 FvH4_5g19220 FvH4_5g19340 FvH4_5g19350 FvH4_5g19360 FvH4_5g23910 FvH4_7g27810
malus_domestica MD00G1001400.v1.1 MD04G1058900.v1.1 MD04G1059300.v1.1 MD04G1059500.v1.1 MD04G1059600.v1.1 MD04G1059800.v1.1 MD04G1061700.v1.1 MD04G1061800.v1.1 MD06G1014800.v1.1 MD06G1052900.v1.1 MD06G1055500.v1.1
prunus_persica Prupe.5G021300_v2.0.a1 Prupe.5G065900_v2.0.a1 Prupe.5G066500_v2.0.a1
pyrus_communis pycom06g04440
rosa_chinensis RchiOBHm_Chr1g0374681 RchiOBHm_Chr2g0120471 RchiOBHm_Chr2g0120711 RchiOBHm_Chr7g0203821 RchiOBHm_Chr7g0203861 RchiOBHm_Chr7g0208401 RchiOBHm_Chr7g0209011 RchiOBHm_Chr7g0213341
rosa_laevigata RLG00000001348 RLG00000002813 RLG00000003173 RLG00000003212 RLG00000003503 RLG00000022835 RLG00000026723 RLG00000035532
rosa_multiflora Rmu_co7960007.1_g000001 Rmu_co7963796.1_g000001 Rmu_co8519951.1_g000002 Rmu_sc0002038.1_g000009 Rmu_sc0004947.1_g000015 Rmu_sc0007490.1_g000005 Rmu_sc0011180.1_g000003 Rmu_sc0015273.1_g000013 Rmu_ssc0000190.1_g000014 Rmu_ssc0000372.1_g000031
rosa_roxburghii Rroxscaffold_3G00246080 Rroxscaffold_3G00249860 Rroxscaffold_3G00249870 Rroxscaffold_3G00253500 Rroxscaffold_4G00283340
rosa_rugosa Rorug01G0384300 Rorug07G0079000 Rorug07G0079000 Rorug07G0079000 Rorug07G0108300 Rorug07G0141200
rosa_samantha Rh1AG395700 Rh1BG359900 Rh1DG006600 Rh1DG390900 Rh2AG287100 Rh3BG335800 Rh3CG321100 Rh5DG116800 Rh7AG273000 Rh7BG207600 Rh7BG207800 Rh7BG237700 Rh7BG268400 Rh7CG221100 Rh7CG221300 Rh7CG254000 Rh7CG257900 Rh7CG292200 Rh7DG215700 Rh7DG215900 Rh7DG244500 Rh7DG249800 Rh7DG280700
rosa_wichuraiana Rw0G010370 Rw1G035090 Rw2G023020 Rw7G018200 Rw7G020570 Rw7G023690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 174
AccB7I CCANNNNNTGG 1 cut(s) 725
AccII CGCG 2 cut(s) 412, 815
AciI CCGC 5 cut(s) 33, 384, 575, 729, 813
AcoI YGGCCR 1 cut(s) 674
AcsI RAATTY 1 cut(s) 287
AfiI CCNNNNNNNGG 4 cut(s) 76, 171, 196, 725
AflII CTTAAG 1 cut(s) 136
AgsI TTSAA 4 cut(s) 196, 379, 634, 706
AjnI CCWGG 1 cut(s) 336
AluBI AGCT 4 cut(s) 169, 580, 688, 781
AluI AGCT 4 cut(s) 169, 580, 688, 781
Alw21I GWGCWC 1 cut(s) 505
Alw26I GTCTC 2 cut(s) 104, 886
AlwNI CAGNNNCTG 1 cut(s) 337
AoxI GGCC 4 cut(s) 334, 593, 674, 818
ApeKI GCWGC 2 cut(s) 64, 827
ApoI RAATTY 1 cut(s) 287
AspLEI GCGC 2 cut(s) 64, 412
AspS9I GGNCC 3 cut(s) 334, 536, 805
AsuC2I CCSGG 1 cut(s) 606
AsuHPI GGTGA 4 cut(s) 92, 283, 450, 620
AsuII TTCGAA 1 cut(s) 210
AvaII GGWCC 2 cut(s) 536, 805
AxyI CCTNAGG 2 cut(s) 507, 540
BalI TGGCCA 1 cut(s) 676
BanI GGYRCC 1 cut(s) 174
BauI CACGAG 1 cut(s) 876
Bbv12I GWGCWC 1 cut(s) 505
BbvCI CCTCAGC 1 cut(s) 831
BbvI GCAGC 2 cut(s) 76, 814
BccI CCATC 4 cut(s) 398, 575, 782, 890
BceAI ACGGC 2 cut(s) 373, 857
BciT130I CCWGG 1 cut(s) 338
BclI TGATCA 1 cut(s) 622
BcnI CCSGG 1 cut(s) 606
BcoDI GTCTC 2 cut(s) 104, 886
BfaI CTAG 2 cut(s) 29, 937
BfrI CTTAAG 1 cut(s) 136
BglII AGATCT 1 cut(s) 767
BisI GCNGC 2 cut(s) 65, 828
BlsI GCNGC 2 cut(s) 66, 829
Bme1390I CCNGG 2 cut(s) 338, 606
Bme18I GGWCC 2 cut(s) 536, 805
BmgT120I GGNCC 3 cut(s) 334, 536, 805
BmiI GGNNCC 3 cut(s) 176, 806, 807
BmrFI CCNGG 2 cut(s) 338, 606
BmsI GCATC 3 cut(s) 321, 853, 923
Bpu10I CCTNAGC 3 cut(s) 170, 444, 831
Bpu14I TTCGAA 1 cut(s) 210
BpuMI CCSGG 1 cut(s) 606
BsaJI CCNNGG 1 cut(s) 294
BsaWI WCCGGW 1 cut(s) 145
Bsc4I CCNNNNNNNGG 4 cut(s) 76, 171, 196, 725
Bse1I ACTGG 1 cut(s) 190
Bse21I CCTNAGG 2 cut(s) 507, 540
Bse3DI GCAATG 1 cut(s) 564
BseBI CCWGG 1 cut(s) 338
BseDI CCNNGG 1 cut(s) 294
BseGI GGATG 5 cut(s) 205, 409, 774, 844, 882
BseLI CCNNNNNNNGG 4 cut(s) 76, 171, 196, 725
BseMI GCAATG 1 cut(s) 564
BseMII CTCAG 4 cut(s) 95, 184, 498, 822
BseNI ACTGG 1 cut(s) 190
BseRI GAGGAG 1 cut(s) 453
BseXI GCAGC 2 cut(s) 76, 814
Bsh1236I CGCG 2 cut(s) 412, 815
Bsh1285I CGRYCG 1 cut(s) 74
BshFI GGCC 4 cut(s) 336, 595, 676, 820
BshNI GGYRCC 1 cut(s) 174
BsiEI CGRYCG 1 cut(s) 74
BsiHKAI GWGCWC 1 cut(s) 505
BsiSI CCGG 2 cut(s) 146, 606
BslFI GGGAC 4 cut(s) 142, 642, 696, 818
BslI CCNNNNNNNGG 4 cut(s) 76, 171, 196, 725
BsmAI GTCTC 2 cut(s) 104, 886
BsmFI GGGAC 4 cut(s) 142, 642, 696, 818
BsmI GAATGC 1 cut(s) 224
BsnI GGCC 4 cut(s) 336, 595, 676, 820
Bsp119I TTCGAA 1 cut(s) 210
Bsp1286I GDGCHC 1 cut(s) 505
Bsp143I GATC 3 cut(s) 262, 622, 767
BspACI CCGC 5 cut(s) 33, 384, 575, 729, 813
BspANI GGCC 4 cut(s) 336, 595, 676, 820
BspCNI CTCAG 4 cut(s) 96, 183, 499, 823
BspFNI CGCG 2 cut(s) 412, 815
BspLI GGNNCC 3 cut(s) 176, 806, 807
BspT104I TTCGAA 1 cut(s) 210
BspT107I GGYRCC 1 cut(s) 174
BspTI CTTAAG 1 cut(s) 136
BsrDI GCAATG 1 cut(s) 564
BsrI ACTGG 1 cut(s) 190
BssECI CCNNGG 1 cut(s) 294
BssMI GATC 3 cut(s) 262, 622, 767
BssSI CACGAG 1 cut(s) 876
Bst2BI CACGAG 1 cut(s) 876
Bst2UI CCWGG 1 cut(s) 338
Bst4CI ACNGT 2 cut(s) 52, 371
BstAFI CTTAAG 1 cut(s) 136
BstAPI GCANNNNNTGC 1 cut(s) 563
BstBI TTCGAA 1 cut(s) 210
BstC8I GCNNGC 3 cut(s) 384, 763, 912
BstDEI CTNAG 7 cut(s) 21, 104, 170, 444, 507, 540, 831
BstF5I GGATG 5 cut(s) 205, 409, 774, 844, 882
BstFNI CGCG 2 cut(s) 412, 815
BstHHI GCGC 2 cut(s) 64, 412
BstKTI GATC 3 cut(s) 265, 625, 770
BstMAI GTCTC 2 cut(s) 104, 886
BstMBI GATC 3 cut(s) 262, 622, 767
BstMCI CGRYCG 1 cut(s) 74
BstMWI GCNNNNNNNGC 3 cut(s) 563, 572, 833
BstNI CCWGG 1 cut(s) 338
BstSCI CCNGG 2 cut(s) 336, 604
BstUI CGCG 2 cut(s) 412, 815
BstV1I GCAGC 2 cut(s) 76, 814
BstX2I RGATCY 1 cut(s) 767
BstYI RGATCY 1 cut(s) 767
Bsu36I CCTNAGG 2 cut(s) 507, 540
BsuRI GGCC 4 cut(s) 336, 595, 676, 820
BtgZI GCGATG 1 cut(s) 761
BtsCI GGATG 5 cut(s) 205, 409, 774, 844, 882
BtsI GCAGTG 2 cut(s) 137, 649
BtsIMutI CAGTG 2 cut(s) 137, 649
Cac8I GCNNGC 3 cut(s) 384, 763, 912
CaiI CAGNNNCTG 1 cut(s) 337
CfoI GCGC 2 cut(s) 64, 412
Cfr13I GGNCC 3 cut(s) 334, 536, 805
CviAII CATG 1 cut(s) 672
DdeI CTNAG 7 cut(s) 21, 104, 170, 444, 507, 540, 831
DpnI GATC 3 cut(s) 264, 624, 769
DpnII GATC 3 cut(s) 262, 622, 767
EaeI YGGCCR 1 cut(s) 674
Eco147I AGGCCT 1 cut(s) 820
Eco47I GGWCC 2 cut(s) 536, 805
Eco81I CCTNAGG 2 cut(s) 507, 540
EcoO109I RGGNCCY 2 cut(s) 334, 805
EcoRII CCWGG 1 cut(s) 336
FaeI CATG 1 cut(s) 675
FaqI GGGAC 4 cut(s) 142, 642, 696, 818
FatI CATG 1 cut(s) 671
FauI CCCGC 2 cut(s) 391, 722
FbaI TGATCA 1 cut(s) 622
Fnu4HI GCNGC 2 cut(s) 65, 828
FokI GGATG 5 cut(s) 212, 416, 761, 831, 869
Fsp4HI GCNGC 2 cut(s) 65, 828
FspBI CTAG 2 cut(s) 29, 937
GlaI GCGC 2 cut(s) 63, 411
GluI GCNGC 2 cut(s) 65, 828
HaeIII GGCC 4 cut(s) 336, 595, 676, 820
HapII CCGG 2 cut(s) 146, 606
HhaI GCGC 2 cut(s) 64, 412
Hin1II CATG 1 cut(s) 675
Hin6I GCGC 2 cut(s) 62, 410
HinP1I GCGC 2 cut(s) 62, 410
HinfI GANTC 5 cut(s) 218, 455, 668, 751, 871
HpaII CCGG 2 cut(s) 146, 606
HphI GGTGA 4 cut(s) 92, 283, 450, 620
Hpy188I TCNGA 4 cut(s) 47, 105, 324, 627
Hpy188III TCNNGA 3 cut(s) 421, 506, 634
Hpy99I CGWCG 1 cut(s) 79
HpyAV CCTTC 3 cut(s) 190, 765, 819
HpyCH4III ACNGT 2 cut(s) 52, 371
HpyCH4IV ACGT 1 cut(s) 480
HpyCH4V TGCA 8 cut(s) 92, 224, 247, 349, 428, 642, 761, 914
HpyF10VI GCNNNNNNNGC 3 cut(s) 563, 572, 833
HpyF3I CTNAG 7 cut(s) 21, 104, 170, 444, 507, 540, 831
HpySE526I ACGT 1 cut(s) 480
Hsp92II CATG 1 cut(s) 675
HspAI GCGC 2 cut(s) 62, 410
KflI GGGWCCC 1 cut(s) 805
Ksp22I TGATCA 1 cut(s) 622
Kzo9I GATC 3 cut(s) 262, 622, 767
LmnI GCTCC 2 cut(s) 508, 577
Lsp1109I GCAGC 2 cut(s) 76, 814
LweI GCATC 3 cut(s) 321, 853, 923
MaeI CTAG 2 cut(s) 29, 937
MaeII ACGT 1 cut(s) 480
MaeIII GTNAC 1 cut(s) 481
MalI GATC 3 cut(s) 264, 624, 769
MboI GATC 3 cut(s) 262, 622, 767
MboII GAAGA 5 cut(s) 199, 311, 509, 780, 844
MflI RGATCY 1 cut(s) 767
MhlI GDGCHC 1 cut(s) 505
MlsI TGGCCA 1 cut(s) 676
MluCI AATT 2 cut(s) 287, 723
MluNI TGGCCA 1 cut(s) 676
MlyI GAGTC 1 cut(s) 677
Mox20I TGGCCA 1 cut(s) 676
MscI TGGCCA 1 cut(s) 676
MseI TTAA 2 cut(s) 137, 399
Msp20I TGGCCA 1 cut(s) 676
MspA1I CMGCKG 1 cut(s) 781
MspCI CTTAAG 1 cut(s) 136
MspI CCGG 2 cut(s) 146, 606
MspR9I CCNGG 2 cut(s) 338, 606
Mva1269I GAATGC 1 cut(s) 224
MvaI CCWGG 1 cut(s) 338
MvnI CGCG 2 cut(s) 412, 815
MwoI GCNNNNNNNGC 3 cut(s) 563, 572, 833
NciI CCSGG 1 cut(s) 606
NdeII GATC 3 cut(s) 262, 622, 767
NlaIII CATG 1 cut(s) 675
NlaIV GGNNCC 3 cut(s) 176, 806, 807
NmuCI GTSAC 1 cut(s) 481
NspV TTCGAA 1 cut(s) 210
PceI AGGCCT 1 cut(s) 820
PcsI WCGNNNNNNNCGW 1 cut(s) 496
PctI GAATGC 1 cut(s) 224
PfeI GAWTC 4 cut(s) 218, 455, 751, 871
PflFI GACNNNGTC 1 cut(s) 479
PflMI CCANNNNNTGG 1 cut(s) 725
PkrI GCNGC 2 cut(s) 66, 829
PleI GAGTC 1 cut(s) 676
PpsI GAGTC 1 cut(s) 676
PpuMI RGGWCCY 1 cut(s) 805
Psp5II RGGWCCY 1 cut(s) 805
Psp6I CCWGG 1 cut(s) 336
PspGI CCWGG 1 cut(s) 336
PspN4I GGNNCC 3 cut(s) 176, 806, 807
PspPI GGNCC 3 cut(s) 334, 536, 805
PspPPI RGGWCCY 1 cut(s) 805
PstNI CAGNNNCTG 1 cut(s) 337
PsuI RGATCY 1 cut(s) 767
PsyI GACNNNGTC 1 cut(s) 479
PvuII CAGCTG 1 cut(s) 781
SaqAI TTAA 2 cut(s) 137, 399
SatI GCNGC 2 cut(s) 65, 828
Sau3AI GATC 3 cut(s) 262, 622, 767
Sau96I GGNCC 3 cut(s) 334, 536, 805
SchI GAGTC 1 cut(s) 677
ScrFI CCNGG 2 cut(s) 338, 606
SduI GDGCHC 1 cut(s) 505
SetI ASST 8 cut(s) 171, 463, 483, 513, 546, 582, 690, 783
SfaNI GCATC 3 cut(s) 321, 853, 923
SfuI TTCGAA 1 cut(s) 210
SinI GGWCC 2 cut(s) 536, 805
SmlI CTYRAG 1 cut(s) 136
SmoI CTYRAG 1 cut(s) 136
Sse9I AATT 2 cut(s) 287, 723
SseBI AGGCCT 1 cut(s) 820
SsiI CCGC 5 cut(s) 33, 384, 575, 729, 813
SspMI CTAG 2 cut(s) 29, 937
StuI AGGCCT 1 cut(s) 820
StyD4I CCNGG 2 cut(s) 336, 604
TaaI ACNGT 2 cut(s) 52, 371
TaiI ACGT 1 cut(s) 483
TaqI TCGA 2 cut(s) 210, 546
TaqII GACCGA 2 cut(s) 88, 510
TasI AATT 2 cut(s) 287, 723
TfiI GAWTC 4 cut(s) 218, 455, 751, 871
Tru1I TTAA 2 cut(s) 137, 399
Tru9I TTAA 2 cut(s) 137, 399
TscAI CASTG 2 cut(s) 137, 649
TseFI GTSAC 1 cut(s) 481
TseI GCWGC 2 cut(s) 64, 827
Tsp45I GTSAC 1 cut(s) 481
TspDTI ATGAA 2 cut(s) 287, 576
TspGWI ACGGA 1 cut(s) 483
TspRI CASTG 2 cut(s) 137, 649
Tth111I GACNNNGTC 1 cut(s) 479
Van91I CCANNNNNTGG 1 cut(s) 725
Vha464I CTTAAG 1 cut(s) 136
VpaK11BI GGWCC 2 cut(s) 536, 805
XapI RAATTY 1 cut(s) 287
XspI CTAG 2 cut(s) 29, 937
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.