Belongs to the protein kinase superfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1159 |
| Acc36I | ACCTGC | 1 cut(s) | 1159 |
| Acc65I | GGTACC | 1 cut(s) | 384 |
| AccB1I | GGYRCC | 1 cut(s) | 384 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 812 |
| AccI | GTMKAC | 1 cut(s) | 374 |
| AccII | CGCG | 1 cut(s) | 1253 |
| AciI | CCGC | 3 cut(s) | 1117, 1139, 1253 |
| AclWI | GGATC | 1 cut(s) | 1213 |
| AcoI | YGGCCR | 1 cut(s) | 462 |
| AcsI | RAATTY | 1 cut(s) | 525 |
| AcuI | CTGAAG | 2 cut(s) | 392, 417 |
| AcvI | CACGTG | 1 cut(s) | 1067 |
| AdeI | CACNNNGTG | 1 cut(s) | 1070 |
| AfaI | GTAC | 4 cut(s) | 59, 359, 386, 402 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 812 |
| AflII | CTTAAG | 1 cut(s) | 272 |
| AflIII | ACRYGT | 1 cut(s) | 1194 |
| AgsI | TTSAA | 4 cut(s) | 242, 344, 988, 1214 |
| AjnI | CCWGG | 3 cut(s) | 957, 1021, 1225 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 541, 573 |
| AleI | CACNNNNGTG | 1 cut(s) | 929 |
| Alw21I | GWGCWC | 2 cut(s) | 394, 995 |
| Alw26I | GTCTC | 2 cut(s) | 859, 1178 |
| AlwI | GGATC | 1 cut(s) | 1213 |
| Ama87I | CYCGRG | 1 cut(s) | 333 |
| AoxI | GGCC | 5 cut(s) | 462, 707, 723, 1016, 1205 |
| ApeKI | GCWGC | 5 cut(s) | 26, 716, 835, 934, 1141 |
| ApoI | RAATTY | 1 cut(s) | 525 |
| AseI | ATTAAT | 1 cut(s) | 114 |
| Asp718I | GGTACC | 1 cut(s) | 384 |
| AspLEI | GCGC | 1 cut(s) | 939 |
| AspS9I | GGNCC | 3 cut(s) | 694, 724, 1017 |
| AsuC2I | CCSGG | 1 cut(s) | 495 |
| AsuHPI | GGTGA | 2 cut(s) | 92, 140 |
| AvaI | CYCGRG | 1 cut(s) | 333 |
| AvaII | GGWCC | 1 cut(s) | 694 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 368, 401 |
| BanI | GGYRCC | 1 cut(s) | 384 |
| BanII | GRGCYC | 3 cut(s) | 394, 995, 1171 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 129, 161 |
| BbrPI | CACGTG | 1 cut(s) | 1067 |
| BbsI | GAAGAC | 2 cut(s) | 134, 470 |
| Bbv12I | GWGCWC | 2 cut(s) | 394, 995 |
| BbvI | GCAGC | 5 cut(s) | 13, 703, 847, 946, 1128 |
| BccI | CCATC | 5 cut(s) | 77, 442, 563, 652, 775 |
| BciT130I | CCWGG | 3 cut(s) | 959, 1023, 1227 |
| BclI | TGATCA | 1 cut(s) | 655 |
| BcnI | CCSGG | 1 cut(s) | 495 |
| BcoDI | GTCTC | 2 cut(s) | 859, 1178 |
| BfaI | CTAG | 4 cut(s) | 651, 795, 846, 885 |
| BfmI | CTRYAG | 2 cut(s) | 717, 918 |
| BfrI | CTTAAG | 1 cut(s) | 272 |
| BfuAI | ACCTGC | 1 cut(s) | 1159 |
| BisI | GCNGC | 5 cut(s) | 27, 717, 836, 935, 1142 |
| BlsI | GCNGC | 5 cut(s) | 28, 718, 837, 936, 1143 |
| BmcAI | AGTACT | 1 cut(s) | 402 |
| Bme1390I | CCNGG | 4 cut(s) | 495, 959, 1023, 1227 |
| Bme18I | GGWCC | 1 cut(s) | 694 |
| BmeT110I | CYCGRG | 1 cut(s) | 333 |
| BmgT120I | GGNCC | 3 cut(s) | 694, 724, 1017 |
| BmiI | GGNNCC | 7 cut(s) | 386, 695, 696, 1019, 1062, 1114, 1168 |
| BmrFI | CCNGG | 4 cut(s) | 495, 959, 1023, 1227 |
| BmrI | ACTGGG | 1 cut(s) | 1165 |
| BmsI | GCATC | 3 cut(s) | 952, 1069, 1091 |
| BmuI | ACTGGG | 1 cut(s) | 1165 |
| BoxI | GACNNNNGTC | 1 cut(s) | 324 |
| BpiI | GAAGAC | 2 cut(s) | 134, 470 |
| BpmI | CTGGAG | 2 cut(s) | 713, 1185 |
| BpuEI | CTTGAG | 1 cut(s) | 182 |
| BpuMI | CCSGG | 1 cut(s) | 495 |
| BsaAI | YACGTR | 1 cut(s) | 1067 |
| BsaI | GGTCTC | 1 cut(s) | 859 |
| BsaJI | CCNNGG | 1 cut(s) | 1021 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 812 |
| Bse1I | ACTGG | 4 cut(s) | 130, 870, 1171, 1192 |
| Bse3DI | GCAATG | 1 cut(s) | 161 |
| BseBI | CCWGG | 3 cut(s) | 959, 1023, 1227 |
| BseDI | CCNNGG | 1 cut(s) | 1021 |
| BseGI | GGATG | 3 cut(s) | 178, 767, 967 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 812 |
| BseMI | GCAATG | 1 cut(s) | 161 |
| BseMII | CTCAG | 2 cut(s) | 1077, 1216 |
| BseNI | ACTGG | 4 cut(s) | 130, 870, 1171, 1192 |
| BseXI | GCAGC | 5 cut(s) | 13, 703, 847, 946, 1128 |
| BsgI | GTGCAG | 1 cut(s) | 953 |
| Bsh1236I | CGCG | 1 cut(s) | 1253 |
| BshFI | GGCC | 5 cut(s) | 464, 709, 725, 1018, 1207 |
| BshNI | GGYRCC | 1 cut(s) | 384 |
| BsiHKAI | GWGCWC | 2 cut(s) | 394, 995 |
| BsiHKCI | CYCGRG | 1 cut(s) | 333 |
| BsiSI | CCGG | 1 cut(s) | 494 |
| BslFI | GGGAC | 3 cut(s) | 707, 762, 874 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 812 |
| BsmAI | GTCTC | 2 cut(s) | 859, 1178 |
| BsmFI | GGGAC | 3 cut(s) | 707, 762, 874 |
| BsnI | GGCC | 5 cut(s) | 464, 709, 725, 1018, 1207 |
| Bso31I | GGTCTC | 1 cut(s) | 859 |
| BsoBI | CYCGRG | 1 cut(s) | 333 |
| Bsp1286I | GDGCHC | 3 cut(s) | 394, 995, 1171 |
| Bsp143I | GATC | 4 cut(s) | 655, 677, 1218, 1232 |
| BspACI | CCGC | 3 cut(s) | 1117, 1139, 1253 |
| BspANI | GGCC | 5 cut(s) | 464, 709, 725, 1018, 1207 |
| BspCNI | CTCAG | 2 cut(s) | 1078, 1215 |
| BspFNI | CGCG | 1 cut(s) | 1253 |
| BspLI | GGNNCC | 7 cut(s) | 386, 695, 696, 1019, 1062, 1114, 1168 |
| BspMAI | CTGCAG | 1 cut(s) | 721 |
| BspMI | ACCTGC | 1 cut(s) | 1159 |
| BspPI | GGATC | 1 cut(s) | 1213 |
| BspQI | GCTCTTC | 1 cut(s) | 983 |
| BspT107I | GGYRCC | 1 cut(s) | 384 |
| BspTI | CTTAAG | 1 cut(s) | 272 |
| BspTNI | GGTCTC | 1 cut(s) | 859 |
| BsrDI | GCAATG | 1 cut(s) | 161 |
| BsrI | ACTGG | 4 cut(s) | 130, 870, 1171, 1192 |
| BssECI | CCNNGG | 1 cut(s) | 1021 |
| BssMI | GATC | 4 cut(s) | 655, 677, 1218, 1232 |
| Bst2UI | CCWGG | 3 cut(s) | 959, 1023, 1227 |
| Bst4CI | ACNGT | 3 cut(s) | 100, 357, 612 |
| Bst6I | CTCTTC | 1 cut(s) | 983 |
| BstAFI | CTTAAG | 1 cut(s) | 272 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1141 |
| BstBAI | YACGTR | 1 cut(s) | 1067 |
| BstC8I | GCNNGC | 3 cut(s) | 108, 912, 1121 |
| BstDEI | CTNAG | 2 cut(s) | 1086, 1202 |
| BstEII | GGTNACC | 1 cut(s) | 292 |
| BstF5I | GGATG | 3 cut(s) | 178, 767, 967 |
| BstFNI | CGCG | 1 cut(s) | 1253 |
| BstHHI | GCGC | 1 cut(s) | 939 |
| BstKTI | GATC | 4 cut(s) | 658, 680, 1221, 1235 |
| BstMAI | GTCTC | 2 cut(s) | 859, 1178 |
| BstMBI | GATC | 4 cut(s) | 655, 677, 1218, 1232 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 452, 461, 722, 907, 1141, 1160 |
| BstNI | CCWGG | 3 cut(s) | 959, 1023, 1227 |
| BstNSI | RCATGY | 1 cut(s) | 914 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 324 |
| BstPI | GGTNACC | 1 cut(s) | 292 |
| BstSCI | CCNGG | 4 cut(s) | 493, 957, 1021, 1225 |
| BstSFI | CTRYAG | 2 cut(s) | 717, 918 |
| BstUI | CGCG | 1 cut(s) | 1253 |
| BstV1I | GCAGC | 5 cut(s) | 13, 703, 847, 946, 1128 |
| BstV2I | GAAGAC | 2 cut(s) | 134, 470 |
| BstX2I | RGATCY | 1 cut(s) | 1218 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1178 |
| BstYI | RGATCY | 1 cut(s) | 1218 |
| BsuRI | GGCC | 5 cut(s) | 464, 709, 725, 1018, 1207 |
| BtgZI | GCGATG | 1 cut(s) | 461 |
| BtsCI | GGATG | 3 cut(s) | 178, 767, 967 |
| BtsI | GCAGTG | 1 cut(s) | 538 |
| BtsIMutI | CAGTG | 5 cut(s) | 8, 360, 538, 877, 1178 |
| BveI | ACCTGC | 1 cut(s) | 1159 |
| Cac8I | GCNNGC | 3 cut(s) | 108, 912, 1121 |
| CfoI | GCGC | 1 cut(s) | 939 |
| Cfr13I | GGNCC | 3 cut(s) | 694, 724, 1017 |
| CseI | GACGC | 1 cut(s) | 1133 |
| Csp6I | GTAC | 4 cut(s) | 58, 358, 385, 401 |
| CviAII | CATG | 7 cut(s) | 218, 460, 561, 659, 911, 1102, 1156 |
| CviQI | GTAC | 4 cut(s) | 58, 358, 385, 401 |
| DdeI | CTNAG | 2 cut(s) | 1086, 1202 |
| DpnI | GATC | 4 cut(s) | 657, 679, 1220, 1234 |
| DpnII | GATC | 4 cut(s) | 655, 677, 1218, 1232 |
| DraIII | CACNNNGTG | 1 cut(s) | 1070 |
| EaeI | YGGCCR | 1 cut(s) | 462 |
| Eam1104I | CTCTTC | 1 cut(s) | 983 |
| EarI | CTCTTC | 1 cut(s) | 983 |
| Ecl136II | GAGCTC | 2 cut(s) | 392, 993 |
| Eco147I | AGGCCT | 1 cut(s) | 1207 |
| Eco24I | GRGCYC | 3 cut(s) | 394, 995, 1171 |
| Eco31I | GGTCTC | 1 cut(s) | 859 |
| Eco32I | GATATC | 1 cut(s) | 592 |
| Eco47I | GGWCC | 1 cut(s) | 694 |
| Eco53kI | GAGCTC | 2 cut(s) | 392, 993 |
| Eco57I | CTGAAG | 2 cut(s) | 392, 417 |
| Eco72I | CACGTG | 1 cut(s) | 1067 |
| Eco88I | CYCGRG | 1 cut(s) | 333 |
| Eco91I | GGTNACC | 1 cut(s) | 292 |
| EcoICRI | GAGCTC | 2 cut(s) | 392, 993 |
| EcoO109I | RGGNCCY | 2 cut(s) | 694, 724 |
| EcoO65I | GGTNACC | 1 cut(s) | 292 |
| EcoRII | CCWGG | 3 cut(s) | 957, 1021, 1225 |
| EcoRV | GATATC | 1 cut(s) | 592 |
| EcoT22I | ATGCAT | 1 cut(s) | 1084 |
| EcoT38I | GRGCYC | 3 cut(s) | 394, 995, 1171 |
| FaeI | CATG | 7 cut(s) | 221, 463, 564, 662, 914, 1105, 1159 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 13, 45, 390, 422 |
| FaqI | GGGAC | 3 cut(s) | 707, 762, 874 |
| FatI | CATG | 7 cut(s) | 217, 459, 560, 658, 910, 1101, 1155 |
| FauI | CCCGC | 2 cut(s) | 1124, 1246 |
| FbaI | TGATCA | 1 cut(s) | 655 |
| FblI | GTMKAC | 1 cut(s) | 374 |
| Fnu4HI | GCNGC | 5 cut(s) | 27, 717, 836, 935, 1142 |
| FokI | GGATG | 3 cut(s) | 165, 754, 974 |
| FriOI | GRGCYC | 3 cut(s) | 394, 995, 1171 |
| Fsp4HI | GCNGC | 5 cut(s) | 27, 717, 836, 935, 1142 |
| FspBI | CTAG | 4 cut(s) | 651, 795, 846, 885 |
| GlaI | GCGC | 1 cut(s) | 938 |
| GluI | GCNGC | 5 cut(s) | 27, 717, 836, 935, 1142 |
| GsuI | CTGGAG | 2 cut(s) | 713, 1185 |
| HaeIII | GGCC | 5 cut(s) | 464, 709, 725, 1018, 1207 |
| HapII | CCGG | 1 cut(s) | 494 |
| HgaI | GACGC | 1 cut(s) | 1133 |
| HhaI | GCGC | 1 cut(s) | 939 |
| Hin1II | CATG | 7 cut(s) | 221, 463, 564, 662, 914, 1105, 1159 |
| Hin6I | GCGC | 1 cut(s) | 937 |
| HinP1I | GCGC | 1 cut(s) | 937 |
| HincII | GTYRAC | 1 cut(s) | 375 |
| HindII | GTYRAC | 1 cut(s) | 375 |
| HinfI | GANTC | 4 cut(s) | 175, 344, 557, 640 |
| HpaII | CCGG | 1 cut(s) | 494 |
| HphI | GGTGA | 2 cut(s) | 92, 140 |
| Hpy166II | GTNNAC | 4 cut(s) | 360, 375, 1048, 1148 |
| Hpy188I | TCNGA | 5 cut(s) | 212, 639, 1078, 1087, 1245 |
| Hpy188III | TCNNGA | 7 cut(s) | 42, 149, 266, 335, 395, 745, 1214 |
| Hpy8I | GTNNAC | 4 cut(s) | 360, 375, 1048, 1148 |
| HpyAV | CCTTC | 3 cut(s) | 708, 782, 906 |
| HpyCH4III | ACNGT | 3 cut(s) | 100, 357, 612 |
| HpyCH4IV | ACGT | 2 cut(s) | 1066, 1194 |
| HpyCH4V | TGCA | 9 cut(s) | 455, 531, 584, 662, 719, 875, 910, 934, 1082 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 452, 461, 722, 907, 1141, 1160 |
| HpyF3I | CTNAG | 2 cut(s) | 1086, 1202 |
| HpySE526I | ACGT | 2 cut(s) | 1066, 1194 |
| Hsp92II | CATG | 7 cut(s) | 221, 463, 564, 662, 914, 1105, 1159 |
| HspAI | GCGC | 1 cut(s) | 937 |
| KflI | GGGWCCC | 1 cut(s) | 694 |
| KpnI | GGTACC | 1 cut(s) | 388 |
| Ksp22I | TGATCA | 1 cut(s) | 655 |
| Kzo9I | GATC | 4 cut(s) | 655, 677, 1218, 1232 |
| LguI | GCTCTTC | 1 cut(s) | 983 |
| LmnI | GCTCC | 4 cut(s) | 397, 824, 832, 1166 |
| Lsp1109I | GCAGC | 5 cut(s) | 13, 703, 847, 946, 1128 |
| LweI | GCATC | 3 cut(s) | 952, 1069, 1091 |
| MaeI | CTAG | 4 cut(s) | 651, 795, 846, 885 |
| MaeII | ACGT | 2 cut(s) | 1066, 1194 |
| MaeIII | GTNAC | 2 cut(s) | 266, 292 |
| MalI | GATC | 4 cut(s) | 657, 679, 1220, 1234 |
| MboI | GATC | 4 cut(s) | 655, 677, 1218, 1232 |
| MboII | GAAGA | 8 cut(s) | 30, 57, 134, 137, 470, 624, 733, 1000 |
| MflI | RGATCY | 1 cut(s) | 1218 |
| MhlI | GDGCHC | 3 cut(s) | 394, 995, 1171 |
| MluCI | AATT | 7 cut(s) | 312, 339, 525, 602, 663, 941, 1107 |
| MmeI | TCCRAC | 2 cut(s) | 810, 1246 |
| MnlI | CCTC | 7 cut(s) | 48, 495, 680, 699, 715, 737, 1051 |
| Mph1103I | ATGCAT | 1 cut(s) | 1084 |
| MseI | TTAA | 5 cut(s) | 65, 114, 156, 273, 305 |
| MslI | CAYNNNNRTG | 1 cut(s) | 929 |
| MspA1I | CMGCKG | 2 cut(s) | 716, 1141 |
| MspCI | CTTAAG | 1 cut(s) | 272 |
| MspI | CCGG | 1 cut(s) | 494 |
| MspR9I | CCNGG | 4 cut(s) | 495, 959, 1023, 1227 |
| MvaI | CCWGG | 3 cut(s) | 959, 1023, 1227 |
| MvnI | CGCG | 1 cut(s) | 1253 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 452, 461, 722, 907, 1141, 1160 |
| NciI | CCSGG | 1 cut(s) | 495 |
| NdeII | GATC | 4 cut(s) | 655, 677, 1218, 1232 |
| NlaIII | CATG | 7 cut(s) | 221, 463, 564, 662, 914, 1105, 1159 |
| NlaIV | GGNNCC | 7 cut(s) | 386, 695, 696, 1019, 1062, 1114, 1168 |
| NmeAIII | GCCGAG | 1 cut(s) | 490 |
| NmuCI | GTSAC | 1 cut(s) | 266 |
| NsiI | ATGCAT | 1 cut(s) | 1084 |
| NspI | RCATGY | 1 cut(s) | 914 |
| OliI | CACNNNNGTG | 1 cut(s) | 929 |
| PaeI | GCATGC | 1 cut(s) | 914 |
| PaqCI | CACCTGC | 1 cut(s) | 1159 |
| PceI | AGGCCT | 1 cut(s) | 1207 |
| PciSI | GCTCTTC | 1 cut(s) | 983 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 385, 1063 |
| PfeI | GAWTC | 4 cut(s) | 175, 344, 557, 640 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 812 |
| PkrI | GCNGC | 5 cut(s) | 28, 718, 837, 936, 1143 |
| PmaCI | CACGTG | 1 cut(s) | 1067 |
| PmlI | CACGTG | 1 cut(s) | 1067 |
| Ppu21I | YACGTR | 1 cut(s) | 1067 |
| PpuMI | RGGWCCY | 1 cut(s) | 694 |
| PshAI | GACNNNNGTC | 1 cut(s) | 324 |
| PshBI | ATTAAT | 1 cut(s) | 114 |
| Psp124BI | GAGCTC | 2 cut(s) | 394, 995 |
| Psp5II | RGGWCCY | 1 cut(s) | 694 |
| Psp6I | CCWGG | 3 cut(s) | 957, 1021, 1225 |
| PspCI | CACGTG | 1 cut(s) | 1067 |
| PspEI | GGTNACC | 1 cut(s) | 292 |
| PspGI | CCWGG | 3 cut(s) | 957, 1021, 1225 |
| PspN4I | GGNNCC | 7 cut(s) | 386, 695, 696, 1019, 1062, 1114, 1168 |
| PspPI | GGNCC | 3 cut(s) | 694, 724, 1017 |
| PspPPI | RGGWCCY | 1 cut(s) | 694 |
| PstI | CTGCAG | 1 cut(s) | 721 |
| PsuI | RGATCY | 1 cut(s) | 1218 |
| PvuII | CAGCTG | 1 cut(s) | 716 |
| RsaI | GTAC | 4 cut(s) | 59, 359, 386, 402 |
| RsaNI | GTAC | 4 cut(s) | 58, 358, 385, 401 |
| RseI | CAYNNNNRTG | 1 cut(s) | 929 |
| SacI | GAGCTC | 2 cut(s) | 394, 995 |
| SalI | GTCGAC | 1 cut(s) | 373 |
| SapI | GCTCTTC | 1 cut(s) | 983 |
| SaqAI | TTAA | 5 cut(s) | 65, 114, 156, 273, 305 |
| SatI | GCNGC | 5 cut(s) | 27, 717, 836, 935, 1142 |
| Sau3AI | GATC | 4 cut(s) | 655, 677, 1218, 1232 |
| Sau96I | GGNCC | 3 cut(s) | 694, 724, 1017 |
| ScaI | AGTACT | 1 cut(s) | 402 |
| ScrFI | CCNGG | 4 cut(s) | 495, 959, 1023, 1227 |
| SduI | GDGCHC | 3 cut(s) | 394, 995, 1171 |
| SfaNI | GCATC | 3 cut(s) | 952, 1069, 1091 |
| SfcI | CTRYAG | 2 cut(s) | 717, 918 |
| SinI | GGWCC | 1 cut(s) | 694 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 929 |
| SmlI | CTYRAG | 2 cut(s) | 197, 272 |
| SmoI | CTYRAG | 2 cut(s) | 197, 272 |
| SphI | GCATGC | 1 cut(s) | 914 |
| Sse9I | AATT | 7 cut(s) | 312, 339, 525, 602, 663, 941, 1107 |
| SseBI | AGGCCT | 1 cut(s) | 1207 |
| SsiI | CCGC | 3 cut(s) | 1117, 1139, 1253 |
| SspI | AATATT | 1 cut(s) | 187 |
| SspMI | CTAG | 4 cut(s) | 651, 795, 846, 885 |
| SstI | GAGCTC | 2 cut(s) | 394, 995 |
| StuI | AGGCCT | 1 cut(s) | 1207 |
| StyD4I | CCNGG | 4 cut(s) | 493, 957, 1021, 1225 |
| TaaI | ACNGT | 3 cut(s) | 100, 357, 612 |
| TaiI | ACGT | 2 cut(s) | 1069, 1197 |
| TaqI | TCGA | 3 cut(s) | 148, 374, 1097 |
| TasI | AATT | 7 cut(s) | 312, 339, 525, 602, 663, 941, 1107 |
| TatI | WGTACW | 1 cut(s) | 400 |
| TfiI | GAWTC | 4 cut(s) | 175, 344, 557, 640 |
| Tru1I | TTAA | 5 cut(s) | 65, 114, 156, 273, 305 |
| Tru9I | TTAA | 5 cut(s) | 65, 114, 156, 273, 305 |
| TscAI | CASTG | 5 cut(s) | 15, 367, 538, 877, 1178 |
| TseFI | GTSAC | 1 cut(s) | 266 |
| TseI | GCWGC | 5 cut(s) | 26, 716, 835, 934, 1141 |
| Tsp45I | GTSAC | 1 cut(s) | 266 |
| TspDTI | ATGAA | 6 cut(s) | 188, 251, 549, 614, 1118, 1172 |
| TspRI | CASTG | 5 cut(s) | 15, 367, 538, 877, 1178 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 812 |
| Vha464I | CTTAAG | 1 cut(s) | 272 |
| VpaK11BI | GGWCC | 1 cut(s) | 694 |
| VspI | ATTAAT | 1 cut(s) | 114 |
| XapI | RAATTY | 1 cut(s) | 525 |
| XceI | RCATGY | 1 cut(s) | 914 |
| XmiI | GTMKAC | 1 cut(s) | 374 |
| XspI | CTAG | 4 cut(s) | 651, 795, 846, 885 |
| ZrmI | AGTACT | 1 cut(s) | 402 |
| Zsp2I | ATGCAT | 1 cut(s) | 1084 |