MD04G1059500.v1.1
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000445
Physical Location & Seq
Forward (+)
7737594 .. 7738016
423 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1059500.v1.1.491

Sequence Viewer

Length: 423 bp
ATGGACAAGTTTTGGATTACTAAGCAACTAGGCGGTGGTTCTTTCGGAACGGTATTTGAGGCGCAGCACCAACCGACGGGTGAAGTCGTTGCAGTCAAGTATCTGAGGGAGACATTCAGTTCCTTTGAGCAGTGCCTAAACCTACCGGAAGTTCAGTCCCTTTCCAAGCTCAGGCACCCCAACATTGTCCAGTTGAAGGATGTCATCTTCGAAAACGATTCTGCATTCCTTGTCTTTGAGTATATGCAGAGCAGTCTGCTTGATCTTATGATGAAAAGGCGAACCAAATTCACCGAGGAAGAAGTTAGAAGCATCTGCTTTCAGATGTTTCAGGGCCTGGCTTATATGCACAGAAACGGCTATTTCCACCGTGATTTGAAGCCCGCTAATGTGTTGGTTAAGGATGGTGTGCGTGTGGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

141

Amino Acids

16.29

Weight (kDa)

7.8

Isoelectric Point (pI)

57.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 6 - 136 1.4e-26 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 7 - 134 3.2e-35 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000445)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G19110 AT4G19110 AT4G19110 AT5G45430 AT5G45430
fragaria_vesca FvH4_2g16890 FvH4_5g19220 FvH4_5g19340 FvH4_5g19350 FvH4_5g19360 FvH4_5g23910 FvH4_7g27810
malus_domestica MD00G1001400.v1.1 MD04G1058900.v1.1 MD04G1059300.v1.1 MD04G1059500.v1.1 MD04G1059600.v1.1 MD04G1059800.v1.1 MD04G1061700.v1.1 MD04G1061800.v1.1 MD06G1014800.v1.1 MD06G1052900.v1.1 MD06G1055500.v1.1
prunus_persica Prupe.5G021300_v2.0.a1 Prupe.5G065900_v2.0.a1 Prupe.5G066500_v2.0.a1
pyrus_communis pycom06g04440
rosa_chinensis RchiOBHm_Chr1g0374681 RchiOBHm_Chr2g0120471 RchiOBHm_Chr2g0120711 RchiOBHm_Chr7g0203821 RchiOBHm_Chr7g0203861 RchiOBHm_Chr7g0208401 RchiOBHm_Chr7g0209011 RchiOBHm_Chr7g0213341
rosa_laevigata RLG00000001348 RLG00000002813 RLG00000003173 RLG00000003212 RLG00000003503 RLG00000022835 RLG00000026723 RLG00000035532
rosa_multiflora Rmu_co7960007.1_g000001 Rmu_co7963796.1_g000001 Rmu_co8519951.1_g000002 Rmu_sc0002038.1_g000009 Rmu_sc0004947.1_g000015 Rmu_sc0007490.1_g000005 Rmu_sc0011180.1_g000003 Rmu_sc0015273.1_g000013 Rmu_ssc0000190.1_g000014 Rmu_ssc0000372.1_g000031
rosa_roxburghii Rroxscaffold_3G00246080 Rroxscaffold_3G00249860 Rroxscaffold_3G00249870 Rroxscaffold_3G00253500 Rroxscaffold_4G00283340
rosa_rugosa Rorug01G0384300 Rorug07G0079000 Rorug07G0079000 Rorug07G0079000 Rorug07G0108300 Rorug07G0141200
rosa_samantha Rh1AG395700 Rh1BG359900 Rh1DG006600 Rh1DG390900 Rh2AG287100 Rh3BG335800 Rh3CG321100 Rh5DG116800 Rh7AG273000 Rh7BG207600 Rh7BG207800 Rh7BG237700 Rh7BG268400 Rh7CG221100 Rh7CG221300 Rh7CG254000 Rh7CG257900 Rh7CG292200 Rh7DG215700 Rh7DG215900 Rh7DG244500 Rh7DG249800 Rh7DG280700
rosa_wichuraiana Rw0G010370 Rw1G035090 Rw2G023020 Rw7G018200 Rw7G020570 Rw7G023690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 174
AciI CCGC 2 cut(s) 33, 384
AcsI RAATTY 1 cut(s) 287
AfiI CCNNNNNNNGG 3 cut(s) 76, 171, 196
AgsI TTSAA 2 cut(s) 196, 379
AjnI CCWGG 1 cut(s) 336
AluBI AGCT 1 cut(s) 169
AluI AGCT 1 cut(s) 169
Alw26I GTCTC 1 cut(s) 104
AlwNI CAGNNNCTG 1 cut(s) 337
AoxI GGCC 1 cut(s) 334
ApeKI GCWGC 1 cut(s) 64
ApoI RAATTY 1 cut(s) 287
AspLEI GCGC 1 cut(s) 64
AspS9I GGNCC 1 cut(s) 334
AsuHPI GGTGA 2 cut(s) 92, 283
AsuII TTCGAA 1 cut(s) 210
BanI GGYRCC 1 cut(s) 174
BbvI GCAGC 1 cut(s) 76
BccI CCATC 1 cut(s) 398
BceAI ACGGC 1 cut(s) 373
BciT130I CCWGG 1 cut(s) 338
BcoDI GTCTC 1 cut(s) 104
BfaI CTAG 1 cut(s) 29
BisI GCNGC 1 cut(s) 65
BlsI GCNGC 1 cut(s) 66
Bme1390I CCNGG 1 cut(s) 338
BmgT120I GGNCC 1 cut(s) 334
BmiI GGNNCC 1 cut(s) 176
BmrFI CCNGG 1 cut(s) 338
BmsI GCATC 1 cut(s) 321
Bpu10I CCTNAGC 1 cut(s) 170
Bpu14I TTCGAA 1 cut(s) 210
BsaJI CCNNGG 1 cut(s) 294
BsaWI WCCGGW 1 cut(s) 145
Bsc4I CCNNNNNNNGG 3 cut(s) 76, 171, 196
Bse1I ACTGG 1 cut(s) 190
BseBI CCWGG 1 cut(s) 338
BseDI CCNNGG 1 cut(s) 294
BseGI GGATG 2 cut(s) 205, 409
BseLI CCNNNNNNNGG 3 cut(s) 76, 171, 196
BseMII CTCAG 2 cut(s) 95, 184
BseNI ACTGG 1 cut(s) 190
BseXI GCAGC 1 cut(s) 76
BshFI GGCC 1 cut(s) 336
BshNI GGYRCC 1 cut(s) 174
BsiSI CCGG 1 cut(s) 146
BslFI GGGAC 1 cut(s) 142
BslI CCNNNNNNNGG 3 cut(s) 76, 171, 196
BsmAI GTCTC 1 cut(s) 104
BsmFI GGGAC 1 cut(s) 142
BsmI GAATGC 1 cut(s) 224
BsnI GGCC 1 cut(s) 336
Bsp119I TTCGAA 1 cut(s) 210
Bsp143I GATC 1 cut(s) 262
BspACI CCGC 2 cut(s) 33, 384
BspANI GGCC 1 cut(s) 336
BspCNI CTCAG 2 cut(s) 96, 183
BspLI GGNNCC 1 cut(s) 176
BspT104I TTCGAA 1 cut(s) 210
BspT107I GGYRCC 1 cut(s) 174
BsrI ACTGG 1 cut(s) 190
BssECI CCNNGG 1 cut(s) 294
BssMI GATC 1 cut(s) 262
Bst2UI CCWGG 1 cut(s) 338
Bst4CI ACNGT 2 cut(s) 52, 371
BstBI TTCGAA 1 cut(s) 210
BstC8I GCNNGC 1 cut(s) 384
BstDEI CTNAG 3 cut(s) 21, 104, 170
BstF5I GGATG 2 cut(s) 205, 409
BstHHI GCGC 1 cut(s) 64
BstKTI GATC 1 cut(s) 265
BstMAI GTCTC 1 cut(s) 104
BstMBI GATC 1 cut(s) 262
BstNI CCWGG 1 cut(s) 338
BstSCI CCNGG 1 cut(s) 336
BstV1I GCAGC 1 cut(s) 76
BsuRI GGCC 1 cut(s) 336
BtsCI GGATG 2 cut(s) 205, 409
BtsI GCAGTG 1 cut(s) 137
BtsIMutI CAGTG 1 cut(s) 137
Cac8I GCNNGC 1 cut(s) 384
CaiI CAGNNNCTG 1 cut(s) 337
CfoI GCGC 1 cut(s) 64
Cfr13I GGNCC 1 cut(s) 334
CviJI RGCY 5 cut(s) 169, 336, 341, 360, 382
CviKI_1 RGCY 5 cut(s) 169, 336, 341, 360, 382
DdeI CTNAG 3 cut(s) 21, 104, 170
DpnI GATC 1 cut(s) 264
DpnII GATC 1 cut(s) 262
EcoO109I RGGNCCY 1 cut(s) 334
EcoRII CCWGG 1 cut(s) 336
FaiI YATR 5 cut(s) 243, 245, 269, 345, 347
FaqI GGGAC 1 cut(s) 142
FauI CCCGC 1 cut(s) 391
Fnu4HI GCNGC 1 cut(s) 65
FokI GGATG 2 cut(s) 212, 416
Fsp4HI GCNGC 1 cut(s) 65
FspBI CTAG 1 cut(s) 29
GlaI GCGC 1 cut(s) 63
GluI GCNGC 1 cut(s) 65
HaeIII GGCC 1 cut(s) 336
HapII CCGG 1 cut(s) 146
HhaI GCGC 1 cut(s) 64
Hin6I GCGC 1 cut(s) 62
HinP1I GCGC 1 cut(s) 62
HinfI GANTC 1 cut(s) 218
HpaII CCGG 1 cut(s) 146
HphI GGTGA 2 cut(s) 92, 283
Hpy188I TCNGA 3 cut(s) 47, 105, 324
Hpy99I CGWCG 1 cut(s) 79
HpyAV CCTTC 1 cut(s) 190
HpyCH4III ACNGT 2 cut(s) 52, 371
HpyCH4V TGCA 4 cut(s) 92, 224, 247, 349
HpyF3I CTNAG 3 cut(s) 21, 104, 170
HspAI GCGC 1 cut(s) 62
Kzo9I GATC 1 cut(s) 262
LpnPI CCDG 6 cut(s) 157, 159, 203, 317, 323, 350
Lsp1109I GCAGC 1 cut(s) 76
LweI GCATC 1 cut(s) 321
MaeI CTAG 1 cut(s) 29
MalI GATC 1 cut(s) 264
MboI GATC 1 cut(s) 262
MboII GAAGA 2 cut(s) 199, 311
MluCI AATT 1 cut(s) 287
MnlI CCTC 3 cut(s) 52, 99, 289
MseI TTAA 1 cut(s) 399
MspI CCGG 1 cut(s) 146
MspR9I CCNGG 1 cut(s) 338
Mva1269I GAATGC 1 cut(s) 224
MvaI CCWGG 1 cut(s) 338
NdeII GATC 1 cut(s) 262
NlaIV GGNNCC 1 cut(s) 176
NspV TTCGAA 1 cut(s) 210
PctI GAATGC 1 cut(s) 224
PfeI GAWTC 1 cut(s) 218
PkrI GCNGC 1 cut(s) 66
Psp6I CCWGG 1 cut(s) 336
PspGI CCWGG 1 cut(s) 336
PspN4I GGNNCC 1 cut(s) 176
PspPI GGNCC 1 cut(s) 334
PstNI CAGNNNCTG 1 cut(s) 337
SaqAI TTAA 1 cut(s) 399
SatI GCNGC 1 cut(s) 65
Sau3AI GATC 1 cut(s) 262
Sau96I GGNCC 1 cut(s) 334
ScrFI CCNGG 1 cut(s) 338
SetI ASST 2 cut(s) 144, 171
SfaNI GCATC 1 cut(s) 321
SfuI TTCGAA 1 cut(s) 210
Sse9I AATT 1 cut(s) 287
SsiI CCGC 2 cut(s) 33, 384
SspMI CTAG 1 cut(s) 29
StyD4I CCNGG 1 cut(s) 336
TaaI ACNGT 2 cut(s) 52, 371
TaqI TCGA 1 cut(s) 210
TasI AATT 1 cut(s) 287
TfiI GAWTC 1 cut(s) 218
Tru1I TTAA 1 cut(s) 399
Tru9I TTAA 1 cut(s) 399
TscAI CASTG 1 cut(s) 137
TseI GCWGC 1 cut(s) 64
TspDTI ATGAA 1 cut(s) 287
TspRI CASTG 1 cut(s) 137
XapI RAATTY 1 cut(s) 287
XspI CTAG 1 cut(s) 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.