MD04G1059600.v1.1
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000445
Physical Location & Seq
Forward (+)
7744056 .. 7744580
525 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1059600.v1.1.491

Sequence Viewer

Length: 525 bp
ATGGTCAAGATTGCAGATTTGGGTTCGGCTAAGGAGATTGATTCACCTCCTCCCTATACAGACTACGTCACTACTCGTCCGTATCGTGCTCCTGAGGTGTTGCTTCGGTCAGGCTTGTATGGTCCTAAGGTCGATATGTGGGCAATGGGTGCCATCATAGCGGAGGTGTTTTCATTCCTGCCTCTATTTCCCGGTGAAAGTGCTGATGATCAGATGTTCAAGATTTGCAGTGTTATAGGCAGTCCCACTTGGGAGTCATGGCCAGAGGGACAGCTTCTTGCCCAAAACTTGAACTATCAGTTTCCAAAAATTGGCGGGGTTGGTCTATCTGCGATGATTCCTTCTGCAAGCAGATCTGCCATCCAGCTGATTTCTTCCCTTTGTTCTTGGGACCCTTACGCGAGGCCTACTGCTGCTGAGGCACTACGGCATCCTTTCTTCGTTGGCAACTACAAGATTCCACGAGCCATCCCTTTGAGACAAAGCAATATCCTGCCTGCATCAAACTCTCTTATTTTCGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

175

Amino Acids

19.06

Weight (kDa)

6.57

Isoelectric Point (pI)

42.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 2 - 147 8.6e-27 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000445)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G19110 AT4G19110 AT4G19110 AT5G45430 AT5G45430
fragaria_vesca FvH4_2g16890 FvH4_5g19220 FvH4_5g19340 FvH4_5g19350 FvH4_5g19360 FvH4_5g23910 FvH4_7g27810
malus_domestica MD00G1001400.v1.1 MD04G1058900.v1.1 MD04G1059300.v1.1 MD04G1059500.v1.1 MD04G1059600.v1.1 MD04G1059800.v1.1 MD04G1061700.v1.1 MD04G1061800.v1.1 MD06G1014800.v1.1 MD06G1052900.v1.1 MD06G1055500.v1.1
prunus_persica Prupe.5G021300_v2.0.a1 Prupe.5G065900_v2.0.a1 Prupe.5G066500_v2.0.a1
pyrus_communis pycom06g04440
rosa_chinensis RchiOBHm_Chr1g0374681 RchiOBHm_Chr2g0120471 RchiOBHm_Chr2g0120711 RchiOBHm_Chr7g0203821 RchiOBHm_Chr7g0203861 RchiOBHm_Chr7g0208401 RchiOBHm_Chr7g0209011 RchiOBHm_Chr7g0213341
rosa_laevigata RLG00000001348 RLG00000002813 RLG00000003173 RLG00000003212 RLG00000003503 RLG00000022835 RLG00000026723 RLG00000035532
rosa_multiflora Rmu_co7960007.1_g000001 Rmu_co7963796.1_g000001 Rmu_co8519951.1_g000002 Rmu_sc0002038.1_g000009 Rmu_sc0004947.1_g000015 Rmu_sc0007490.1_g000005 Rmu_sc0011180.1_g000003 Rmu_sc0015273.1_g000013 Rmu_ssc0000190.1_g000014 Rmu_ssc0000372.1_g000031
rosa_roxburghii Rroxscaffold_3G00246080 Rroxscaffold_3G00249860 Rroxscaffold_3G00249870 Rroxscaffold_3G00253500 Rroxscaffold_4G00283340
rosa_rugosa Rorug01G0384300 Rorug07G0079000 Rorug07G0079000 Rorug07G0079000 Rorug07G0108300 Rorug07G0141200
rosa_samantha Rh1AG395700 Rh1BG359900 Rh1DG006600 Rh1DG390900 Rh2AG287100 Rh3BG335800 Rh3CG321100 Rh5DG116800 Rh7AG273000 Rh7BG207600 Rh7BG207800 Rh7BG237700 Rh7BG268400 Rh7CG221100 Rh7CG221300 Rh7CG254000 Rh7CG257900 Rh7CG292200 Rh7DG215700 Rh7DG215900 Rh7DG244500 Rh7DG249800 Rh7DG280700
rosa_wichuraiana Rw0G010370 Rw1G035090 Rw2G023020 Rw7G018200 Rw7G020570 Rw7G023690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 149
AccB7I CCANNNNNTGG 1 cut(s) 311
AccII CGCG 1 cut(s) 401
AciI CCGC 2 cut(s) 161, 315
AcoI YGGCCR 1 cut(s) 260
AfiI CCNNNNNNNGG 1 cut(s) 311
AgsI TTSAA 2 cut(s) 220, 292
AluBI AGCT 2 cut(s) 274, 367
AluI AGCT 2 cut(s) 274, 367
Alw21I GWGCWC 1 cut(s) 91
Alw26I GTCTC 1 cut(s) 472
AoxI GGCC 2 cut(s) 260, 404
ApeKI GCWGC 1 cut(s) 413
AspS9I GGNCC 2 cut(s) 122, 391
AsuC2I CCSGG 1 cut(s) 192
AsuHPI GGTGA 2 cut(s) 36, 206
AvaII GGWCC 2 cut(s) 122, 391
AxyI CCTNAGG 2 cut(s) 93, 126
BalI TGGCCA 1 cut(s) 262
BanI GGYRCC 1 cut(s) 149
BauI CACGAG 1 cut(s) 462
Bbv12I GWGCWC 1 cut(s) 91
BbvCI CCTCAGC 1 cut(s) 417
BbvI GCAGC 1 cut(s) 400
BccI CCATC 3 cut(s) 161, 368, 476
BceAI ACGGC 1 cut(s) 443
BclI TGATCA 1 cut(s) 208
BcnI CCSGG 1 cut(s) 192
BcoDI GTCTC 1 cut(s) 472
BfaI CTAG 1 cut(s) 523
BglII AGATCT 1 cut(s) 353
BisI GCNGC 1 cut(s) 414
BlsI GCNGC 1 cut(s) 415
Bme1390I CCNGG 1 cut(s) 192
Bme18I GGWCC 2 cut(s) 122, 391
BmgT120I GGNCC 2 cut(s) 122, 391
BmiI GGNNCC 3 cut(s) 151, 392, 393
BmrFI CCNGG 1 cut(s) 192
BmsI GCATC 2 cut(s) 439, 509
Bpu10I CCTNAGC 2 cut(s) 30, 417
BpuMI CCSGG 1 cut(s) 192
Bsc4I CCNNNNNNNGG 1 cut(s) 311
Bse21I CCTNAGG 2 cut(s) 93, 126
Bse3DI GCAATG 1 cut(s) 150
BseGI GGATG 3 cut(s) 360, 430, 468
BseLI CCNNNNNNNGG 1 cut(s) 311
BseMI GCAATG 1 cut(s) 150
BseMII CTCAG 2 cut(s) 84, 408
BseRI GAGGAG 1 cut(s) 39
BseXI GCAGC 1 cut(s) 400
Bsh1236I CGCG 1 cut(s) 401
BshFI GGCC 2 cut(s) 262, 406
BshNI GGYRCC 1 cut(s) 149
BsiHKAI GWGCWC 1 cut(s) 91
BsiSI CCGG 1 cut(s) 192
BslFI GGGAC 3 cut(s) 228, 282, 404
BslI CCNNNNNNNGG 1 cut(s) 311
BsmAI GTCTC 1 cut(s) 472
BsmFI GGGAC 3 cut(s) 228, 282, 404
BsnI GGCC 2 cut(s) 262, 406
Bsp1286I GDGCHC 1 cut(s) 91
Bsp143I GATC 2 cut(s) 208, 353
BspACI CCGC 2 cut(s) 161, 315
BspANI GGCC 2 cut(s) 262, 406
BspCNI CTCAG 2 cut(s) 85, 409
BspFNI CGCG 1 cut(s) 401
BspLI GGNNCC 3 cut(s) 151, 392, 393
BspT107I GGYRCC 1 cut(s) 149
BsrDI GCAATG 1 cut(s) 150
BssMI GATC 2 cut(s) 208, 353
BssSI CACGAG 1 cut(s) 462
Bst2BI CACGAG 1 cut(s) 462
BstAPI GCANNNNNTGC 1 cut(s) 149
BstC8I GCNNGC 2 cut(s) 349, 498
BstDEI CTNAG 4 cut(s) 30, 93, 126, 417
BstF5I GGATG 3 cut(s) 360, 430, 468
BstFNI CGCG 1 cut(s) 401
BstKTI GATC 2 cut(s) 211, 356
BstMAI GTCTC 1 cut(s) 472
BstMBI GATC 2 cut(s) 208, 353
BstMWI GCNNNNNNNGC 3 cut(s) 149, 158, 419
BstSCI CCNGG 1 cut(s) 190
BstUI CGCG 1 cut(s) 401
BstV1I GCAGC 1 cut(s) 400
BstX2I RGATCY 1 cut(s) 353
BstYI RGATCY 1 cut(s) 353
Bsu36I CCTNAGG 2 cut(s) 93, 126
BsuRI GGCC 2 cut(s) 262, 406
BtgZI GCGATG 1 cut(s) 347
BtsCI GGATG 3 cut(s) 360, 430, 468
BtsI GCAGTG 1 cut(s) 235
BtsIMutI CAGTG 1 cut(s) 235
Cac8I GCNNGC 2 cut(s) 349, 498
Cfr13I GGNCC 2 cut(s) 122, 391
CviAII CATG 1 cut(s) 258
CviJI RGCY 7 cut(s) 29, 114, 262, 274, 367, 406, 467
CviKI_1 RGCY 7 cut(s) 29, 114, 262, 274, 367, 406, 467
DdeI CTNAG 4 cut(s) 30, 93, 126, 417
DpnI GATC 2 cut(s) 210, 355
DpnII GATC 2 cut(s) 208, 353
EaeI YGGCCR 1 cut(s) 260
Eco147I AGGCCT 1 cut(s) 406
Eco47I GGWCC 2 cut(s) 122, 391
Eco81I CCTNAGG 2 cut(s) 93, 126
EcoO109I RGGNCCY 1 cut(s) 391
FaeI CATG 1 cut(s) 261
FaiI YATR 6 cut(s) 57, 120, 137, 158, 236, 259
FaqI GGGAC 3 cut(s) 228, 282, 404
FatI CATG 1 cut(s) 257
FauI CCCGC 1 cut(s) 308
FbaI TGATCA 1 cut(s) 208
Fnu4HI GCNGC 1 cut(s) 414
FokI GGATG 3 cut(s) 347, 417, 455
Fsp4HI GCNGC 1 cut(s) 414
FspBI CTAG 1 cut(s) 523
GluI GCNGC 1 cut(s) 414
HaeIII GGCC 2 cut(s) 262, 406
HapII CCGG 1 cut(s) 192
Hin1II CATG 1 cut(s) 261
HinfI GANTC 4 cut(s) 41, 254, 337, 457
HpaII CCGG 1 cut(s) 192
HphI GGTGA 2 cut(s) 36, 206
Hpy188I TCNGA 1 cut(s) 213
Hpy188III TCNNGA 3 cut(s) 7, 92, 220
HpyAV CCTTC 1 cut(s) 351
HpyCH4IV ACGT 1 cut(s) 66
HpyCH4V TGCA 4 cut(s) 14, 228, 347, 500
HpyF10VI GCNNNNNNNGC 3 cut(s) 149, 158, 419
HpyF3I CTNAG 4 cut(s) 30, 93, 126, 417
HpySE526I ACGT 1 cut(s) 66
Hsp92II CATG 1 cut(s) 261
KflI GGGWCCC 1 cut(s) 391
Ksp22I TGATCA 1 cut(s) 208
Kzo9I GATC 2 cut(s) 208, 353
LmnI GCTCC 1 cut(s) 94
LpnPI CCDG 8 cut(s) 96, 105, 191, 205, 276, 377, 506, 510
Lsp1109I GCAGC 1 cut(s) 400
LweI GCATC 2 cut(s) 439, 509
MaeI CTAG 1 cut(s) 523
MaeII ACGT 1 cut(s) 66
MaeIII GTNAC 1 cut(s) 67
MalI GATC 2 cut(s) 210, 355
MboI GATC 2 cut(s) 208, 353
MboII GAAGA 2 cut(s) 366, 430
MflI RGATCY 1 cut(s) 353
MhlI GDGCHC 1 cut(s) 91
MlsI TGGCCA 1 cut(s) 262
MluCI AATT 1 cut(s) 309
MluNI TGGCCA 1 cut(s) 262
MlyI GAGTC 1 cut(s) 263
MnlI CCTC 8 cut(s) 57, 60, 88, 157, 192, 259, 396, 412
Mox20I TGGCCA 1 cut(s) 262
MscI TGGCCA 1 cut(s) 262
Msp20I TGGCCA 1 cut(s) 262
MspA1I CMGCKG 1 cut(s) 367
MspI CCGG 1 cut(s) 192
MspR9I CCNGG 1 cut(s) 192
MvnI CGCG 1 cut(s) 401
MwoI GCNNNNNNNGC 3 cut(s) 149, 158, 419
NciI CCSGG 1 cut(s) 192
NdeII GATC 2 cut(s) 208, 353
NlaIII CATG 1 cut(s) 261
NlaIV GGNNCC 3 cut(s) 151, 392, 393
NmuCI GTSAC 1 cut(s) 67
PceI AGGCCT 1 cut(s) 406
PcsI WCGNNNNNNNCGW 1 cut(s) 82
PfeI GAWTC 3 cut(s) 41, 337, 457
PflFI GACNNNGTC 1 cut(s) 65
PflMI CCANNNNNTGG 1 cut(s) 311
PkrI GCNGC 1 cut(s) 415
PleI GAGTC 1 cut(s) 262
PpsI GAGTC 1 cut(s) 262
PpuMI RGGWCCY 1 cut(s) 391
Psp5II RGGWCCY 1 cut(s) 391
PspN4I GGNNCC 3 cut(s) 151, 392, 393
PspPI GGNCC 2 cut(s) 122, 391
PspPPI RGGWCCY 1 cut(s) 391
PsuI RGATCY 1 cut(s) 353
PsyI GACNNNGTC 1 cut(s) 65
PvuII CAGCTG 1 cut(s) 367
SatI GCNGC 1 cut(s) 414
Sau3AI GATC 2 cut(s) 208, 353
Sau96I GGNCC 2 cut(s) 122, 391
SchI GAGTC 1 cut(s) 263
ScrFI CCNGG 1 cut(s) 192
SduI GDGCHC 1 cut(s) 91
SetI ASST 7 cut(s) 49, 69, 99, 132, 168, 276, 369
SfaNI GCATC 2 cut(s) 439, 509
SinI GGWCC 2 cut(s) 122, 391
Sse9I AATT 1 cut(s) 309
SseBI AGGCCT 1 cut(s) 406
SsiI CCGC 2 cut(s) 161, 315
SspMI CTAG 1 cut(s) 523
StuI AGGCCT 1 cut(s) 406
StyD4I CCNGG 1 cut(s) 190
TaiI ACGT 1 cut(s) 69
TaqI TCGA 1 cut(s) 132
TaqII GACCGA 1 cut(s) 96
TasI AATT 1 cut(s) 309
TfiI GAWTC 3 cut(s) 41, 337, 457
TscAI CASTG 1 cut(s) 235
TseFI GTSAC 1 cut(s) 67
TseI GCWGC 1 cut(s) 413
Tsp45I GTSAC 1 cut(s) 67
TspDTI ATGAA 1 cut(s) 162
TspGWI ACGGA 1 cut(s) 69
TspRI CASTG 1 cut(s) 235
Tth111I GACNNNGTC 1 cut(s) 65
Van91I CCANNNNNTGG 1 cut(s) 311
VpaK11BI GGWCC 2 cut(s) 122, 391
XspI CTAG 1 cut(s) 523
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.