Rh1DG391200

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0005396
Physical Location & Seq
Forward (+)
60954252 .. 60954911
660 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG391200.1

Sequence Viewer

Length: 660 bp
ATGCCTATAAATTGGTGTTGGCCTCTCACACAGTCACCCATCAATCCGAGAAAAACAAAAATGGGAGCTCAAACTCTCATATCTCATGTCACTCTTCTCCCATTTATCATTATCATCATCATGATAAACATGCACACAACCATGGCAGCCTCCTCTTCAACCCCAGAACTTCACAACTCCTATCTCCTAAGAGCTTGCAATTCAACCACATACCCACAAATTTGCTACAAGTCCCTCTTCTCCTACGCCTCCACAATCAACTCAAACCCTCAGAAGCTCTGCAGCTACGCCCTCTCTGTCGCCCTTAAAGCCGCCCGCAACGCCTCCTCCACCGTGACGAAGTTGTCCAAGAGGAATGGATTGACACACTCAGAGGCTTTGGTCGTCAAGGATTGCATAGAGAATATCAGGGACTCCATTAACGAGCTCAAGCAGTCCGTGAGTGCCATGAGCAACTTGGGAGGTGCCAATATGGAATACCAGTTGGGTGACATAAAGACTTGGGTCAGCGCTGCCCTAACAGACGATGATACTTGTATGGAAGGGTTTGATGAGGTGAAGGTGAGCTTGGATGTGAAGAACGCCATCAGTAAGAGCATCATCAATATTGCTAGATTGGCAAGTAATGCCCTGTCTCTCATCAACAGCCTCAATTATTAG

Protein Analysis

219

Amino Acids

24.01

Weight (kDa)

8.71

Isoelectric Point (pI)

44.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 64 - 213 1.1e-34 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 343
AccB1I GGYRCC 1 cut(s) 464
AciI CCGC 2 cut(s) 312, 316
AcsI RAATTY 1 cut(s) 219
AfeI AGCGCT 1 cut(s) 511
AgsI TTSAA 2 cut(s) 159, 204
AjuI GAANNNNNNNTTGG 2 cut(s) 551, 583
AluBI AGCT 6 cut(s) 68, 194, 277, 285, 427, 567
AluI AGCT 6 cut(s) 68, 194, 277, 285, 427, 567
Alw21I GWGCWC 2 cut(s) 70, 429
Alw26I GTCTC 1 cut(s) 639
Aor51HI AGCGCT 1 cut(s) 511
AoxI GGCC 1 cut(s) 20
ApeKI GCWGC 3 cut(s) 146, 282, 512
ApoI RAATTY 1 cut(s) 219
AspLEI GCGC 1 cut(s) 512
AsuHPI GGTGA 4 cut(s) 27, 500, 568, 574
BanI GGYRCC 1 cut(s) 464
BanII GRGCYC 2 cut(s) 70, 429
Bbv12I GWGCWC 2 cut(s) 70, 429
BbvI GCAGC 3 cut(s) 158, 294, 499
BccI CCATC 2 cut(s) 47, 593
BcoDI GTCTC 1 cut(s) 639
BfaI CTAG 1 cut(s) 612
BfmI CTRYAG 1 cut(s) 280
BfoI RGCGCY 1 cut(s) 513
BisI GCNGC 4 cut(s) 147, 283, 312, 513
BlsI GCNGC 4 cut(s) 148, 284, 313, 514
BmiI GGNNCC 1 cut(s) 466
BmsI GCATC 1 cut(s) 606
BoxI GACNNNNGTC 1 cut(s) 503
BpuEI CTTGAG 1 cut(s) 413
BsaJI CCNNGG 1 cut(s) 141
BsaXI ACNNNNNCTCC 2 cut(s) 311, 341
Bse1I ACTGG 1 cut(s) 481
BseDI CCNNGG 1 cut(s) 141
BseGI GGATG 1 cut(s) 577
BseMII CTCAG 2 cut(s) 284, 384
BseNI ACTGG 1 cut(s) 481
BseRI GAGGAG 2 cut(s) 142, 316
BseXI GCAGC 3 cut(s) 158, 294, 499
BshFI GGCC 1 cut(s) 22
BshNI GGYRCC 1 cut(s) 464
BsiHKAI GWGCWC 2 cut(s) 70, 429
BslFI GGGAC 2 cut(s) 217, 425
BsmAI GTCTC 1 cut(s) 639
BsmFI GGGAC 2 cut(s) 217, 425
BsnI GGCC 1 cut(s) 22
Bsp1286I GDGCHC 2 cut(s) 70, 429
Bsp19I CCATGG 1 cut(s) 141
BspACI CCGC 2 cut(s) 312, 316
BspANI GGCC 1 cut(s) 22
BspCNI CTCAG 2 cut(s) 283, 383
BspHI TCATGA 1 cut(s) 120
BspLI GGNNCC 1 cut(s) 466
BspMAI CTGCAG 1 cut(s) 284
BspT107I GGYRCC 1 cut(s) 464
BsrI ACTGG 1 cut(s) 481
BssECI CCNNGG 1 cut(s) 141
BssT1I CCWWGG 1 cut(s) 141
Bst4CI ACNGT 2 cut(s) 33, 334
Bst6I CTCTTC 3 cut(s) 99, 160, 242
BstAPI GCANNNNNTGC 1 cut(s) 626
BstC8I GCNNGC 2 cut(s) 196, 316
BstDEI CTNAG 3 cut(s) 188, 270, 370
BstDSI CCRYGG 1 cut(s) 141
BstF5I GGATG 1 cut(s) 577
BstH2I RGCGCY 1 cut(s) 513
BstHHI GCGC 1 cut(s) 512
BstMAI GTCTC 1 cut(s) 639
BstMWI GCNNNNNNNGC 4 cut(s) 308, 320, 617, 626
BstNSI RCATGY 1 cut(s) 133
BstPAI GACNNNNGTC 1 cut(s) 503
BstSFI CTRYAG 1 cut(s) 280
BstV1I GCAGC 3 cut(s) 158, 294, 499
BsuRI GGCC 1 cut(s) 22
BtgI CCRYGG 1 cut(s) 141
BtsCI GGATG 1 cut(s) 577
Cac8I GCNNGC 2 cut(s) 196, 316
CciI TCATGA 1 cut(s) 120
CfoI GCGC 1 cut(s) 512
CviAII CATG 5 cut(s) 86, 121, 130, 142, 448
DdeI CTNAG 3 cut(s) 188, 270, 370
DrdI GACNNNNNNGTC 1 cut(s) 343
DseDI GACNNNNNNGTC 1 cut(s) 343
Eam1104I CTCTTC 3 cut(s) 99, 160, 242
EarI CTCTTC 3 cut(s) 99, 160, 242
Ecl136II GAGCTC 2 cut(s) 68, 427
Eco130I CCWWGG 1 cut(s) 141
Eco24I GRGCYC 2 cut(s) 70, 429
Eco47III AGCGCT 1 cut(s) 511
Eco53kI GAGCTC 2 cut(s) 68, 427
EcoICRI GAGCTC 2 cut(s) 68, 427
EcoT14I CCWWGG 1 cut(s) 141
EcoT38I GRGCYC 2 cut(s) 70, 429
ErhI CCWWGG 1 cut(s) 141
FaeI CATG 5 cut(s) 89, 124, 133, 145, 451
FalI AAGNNNNNCTT 4 cut(s) 221, 253, 551, 583
FaqI GGGAC 2 cut(s) 217, 425
FatI CATG 5 cut(s) 85, 120, 129, 141, 447
FauI CCCGC 1 cut(s) 323
Fnu4HI GCNGC 4 cut(s) 147, 283, 312, 513
FokI GGATG 1 cut(s) 584
FriOI GRGCYC 2 cut(s) 70, 429
Fsp4HI GCNGC 4 cut(s) 147, 283, 312, 513
FspBI CTAG 1 cut(s) 612
GlaI GCGC 1 cut(s) 511
GluI GCNGC 4 cut(s) 147, 283, 312, 513
HaeII RGCGCY 1 cut(s) 513
HaeIII GGCC 1 cut(s) 22
HhaI GCGC 1 cut(s) 512
Hin1II CATG 5 cut(s) 89, 124, 133, 145, 451
Hin6I GCGC 1 cut(s) 510
HinP1I GCGC 1 cut(s) 510
HinfI GANTC 1 cut(s) 413
HphI GGTGA 4 cut(s) 27, 500, 568, 574
Hpy188I TCNGA 3 cut(s) 48, 273, 373
Hpy188III TCNNGA 1 cut(s) 121
HpyAV CCTTC 2 cut(s) 536, 553
HpyCH4III ACNGT 2 cut(s) 33, 334
HpyCH4V TGCA 4 cut(s) 133, 198, 282, 396
HpyF10VI GCNNNNNNNGC 4 cut(s) 308, 320, 617, 626
HpyF3I CTNAG 3 cut(s) 188, 270, 370
Hsp92II CATG 5 cut(s) 89, 124, 133, 145, 451
HspAI GCGC 1 cut(s) 510
LmnI GCTCC 1 cut(s) 65
LpnPI CCDG 4 cut(s) 177, 394, 494, 644
Lsp1109I GCAGC 3 cut(s) 158, 294, 499
LweI GCATC 1 cut(s) 606
MaeI CTAG 1 cut(s) 612
MaeIII GTNAC 4 cut(s) 33, 88, 334, 488
MboII GAAGA 4 cut(s) 86, 147, 229, 589
MhlI GDGCHC 2 cut(s) 70, 429
MluCI AATT 4 cut(s) 10, 199, 219, 652
MlyI GAGTC 1 cut(s) 407
MseI TTAA 2 cut(s) 306, 420
MslI CAYNNNNRTG 2 cut(s) 119, 140
MwoI GCNNNNNNNGC 4 cut(s) 308, 320, 617, 626
NcoI CCATGG 1 cut(s) 141
NlaIII CATG 5 cut(s) 89, 124, 133, 145, 451
NlaIV GGNNCC 1 cut(s) 466
NmuCI GTSAC 4 cut(s) 33, 88, 334, 488
NspI RCATGY 1 cut(s) 133
PagI TCATGA 1 cut(s) 120
PkrI GCNGC 4 cut(s) 148, 284, 313, 514
PleI GAGTC 1 cut(s) 407
PpsI GAGTC 1 cut(s) 407
PshAI GACNNNNGTC 1 cut(s) 503
Psp124BI GAGCTC 2 cut(s) 70, 429
PspN4I GGNNCC 1 cut(s) 466
PstI CTGCAG 1 cut(s) 284
RseI CAYNNNNRTG 2 cut(s) 119, 140
SacI GAGCTC 2 cut(s) 70, 429
SaqAI TTAA 2 cut(s) 306, 420
SatI GCNGC 4 cut(s) 147, 283, 312, 513
SchI GAGTC 1 cut(s) 407
SduI GDGCHC 2 cut(s) 70, 429
SetI ASST 9 cut(s) 70, 196, 279, 287, 429, 466, 558, 564, 569
SfaNI GCATC 1 cut(s) 606
SfcI CTRYAG 1 cut(s) 280
SmiMI CAYNNNNRTG 2 cut(s) 119, 140
SmlI CTYRAG 1 cut(s) 428
SmoI CTYRAG 1 cut(s) 428
Sse9I AATT 4 cut(s) 10, 199, 219, 652
SsiI CCGC 2 cut(s) 312, 316
SspI AATATT 1 cut(s) 607
SspMI CTAG 1 cut(s) 612
SstI GAGCTC 2 cut(s) 70, 429
StyI CCWWGG 1 cut(s) 141
TaaI ACNGT 2 cut(s) 33, 334
TasI AATT 4 cut(s) 10, 199, 219, 652
TauI GCSGC 1 cut(s) 314
Tru1I TTAA 2 cut(s) 306, 420
Tru9I TTAA 2 cut(s) 306, 420
TseFI GTSAC 4 cut(s) 33, 88, 334, 488
TseI GCWGC 3 cut(s) 146, 282, 512
Tsp45I GTSAC 4 cut(s) 33, 88, 334, 488
TspGWI ACGGA 1 cut(s) 427
XapI RAATTY 1 cut(s) 219
XceI RCATGY 1 cut(s) 133
XcmI CCANNNNNNNNNTGG 1 cut(s) 454
XspI CTAG 1 cut(s) 612
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.