Rroxscaffold_4G00283300

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0005396
Physical Location & Seq
Reverse (-)
5253569 .. 5254334
766 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00283300.1

Sequence Viewer

Length: 573 bp
ATGGGAGCTCCCACATTTCTCTCCACTCTTCTCCTATTCATCCTCTTCATCACGACCACGGCTACTTCTTCTTCGAGCCAAACCTACAAAACCTACGTCAAAACAGCCTGCAACTCTACCACGTACCCAGAAATCTGCTACAAGACTCTCTCCTCCTACGCCACATCCGTCAAATCAAACCCTCAGAAGCTCTGCAACTATGCCCTCTCCGTAACCCTAAAAGCCGCGAAAAAAGCCTCTTCCACGGTGTCGAAGCTGTCCAAGAAGAAAGGATTGACCAAAGCAGAGGCCGGGGTGGTGGAGGATTGCATAGAGAACATGGAGGACTCCGTCGACGAGCTCAAACAGGCGGTGACTGCCATGAAAAATTTGGGAGATGCGGATGTCCAACTTCATTTGGATGACATAAAGACTTGGGTTAGTGCTTCGCTGACGGATGAGGACACCTGTACGGATGGATTCGATGAGCAGAAGGTAAGTGCGGCGGTGAAGAACACCATTAATAAGAGCATTCTCACTGTTGCTAGATTGGCTAGCAATGCTTTATCTCTCATCAACAACCTCAGTTACTAG

Protein Analysis

190

Amino Acids

20.48

Weight (kDa)

7.54

Isoelectric Point (pI)

31.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 32 - 184 9.2e-39 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 333
AccII CGCG 1 cut(s) 227
AciI CCGC 5 cut(s) 225, 350, 380, 482, 485
AcsI RAATTY 1 cut(s) 367
AfaI GTAC 2 cut(s) 125, 451
AluBI AGCT 4 cut(s) 8, 190, 256, 340
AluI AGCT 4 cut(s) 8, 190, 256, 340
Alw21I GWGCWC 2 cut(s) 10, 342
AoxI GGCC 1 cut(s) 288
ApoI RAATTY 1 cut(s) 367
ArsI GACNNNNNNTTYG 2 cut(s) 81, 113
AseI ATTAAT 1 cut(s) 501
AsuC2I CCSGG 1 cut(s) 292
AsuHPI GGTGA 2 cut(s) 364, 499
AsuNHI GCTAGC 1 cut(s) 533
BanII GRGCYC 2 cut(s) 10, 342
Bbv12I GWGCWC 2 cut(s) 10, 342
BccI CCATC 1 cut(s) 449
BceAI ACGGC 1 cut(s) 75
BcnI CCSGG 1 cut(s) 292
BfaI CTAG 3 cut(s) 525, 534, 571
BisI GCNGC 2 cut(s) 225, 483
BlsI GCNGC 2 cut(s) 226, 484
Bme1390I CCNGG 1 cut(s) 292
BmrFI CCNGG 1 cut(s) 292
BmsI GCATC 1 cut(s) 367
BmtI GCTAGC 1 cut(s) 537
BpuMI CCSGG 1 cut(s) 292
BsaAI YACGTR 1 cut(s) 123
BsaJI CCNNGG 3 cut(s) 57, 243, 291
Bse3DI GCAATG 1 cut(s) 544
BseDI CCNNGG 3 cut(s) 57, 243, 291
BseGI GGATG 6 cut(s) 39, 164, 388, 406, 442, 460
BseMI GCAATG 1 cut(s) 544
BseMII CTCAG 1 cut(s) 197
BseRI GAGGAG 1 cut(s) 142
Bsh1236I CGCG 1 cut(s) 227
BshFI GGCC 1 cut(s) 290
BsiHKAI GWGCWC 2 cut(s) 10, 342
BsiSI CCGG 1 cut(s) 291
BsmI GAATGC 1 cut(s) 510
BsnI GGCC 1 cut(s) 290
Bsp1286I GDGCHC 2 cut(s) 10, 342
BspACI CCGC 5 cut(s) 225, 350, 380, 482, 485
BspANI GGCC 1 cut(s) 290
BspCNI CTCAG 1 cut(s) 196
BspFNI CGCG 1 cut(s) 227
BspOI GCTAGC 1 cut(s) 537
BsrDI GCAATG 1 cut(s) 544
BssECI CCNNGG 3 cut(s) 57, 243, 291
Bst4CI ACNGT 2 cut(s) 247, 520
Bst6I CTCTTC 3 cut(s) 33, 50, 244
BstBAI YACGTR 1 cut(s) 123
BstC8I GCNNGC 2 cut(s) 109, 535
BstDEI CTNAG 2 cut(s) 183, 563
BstDSI CCRYGG 2 cut(s) 57, 243
BstF5I GGATG 6 cut(s) 39, 164, 388, 406, 442, 460
BstFNI CGCG 1 cut(s) 227
BstMWI GCNNNNNNNGC 4 cut(s) 233, 356, 530, 539
BstSCI CCNGG 1 cut(s) 290
BstUI CGCG 1 cut(s) 227
BsuRI GGCC 1 cut(s) 290
BtgI CCRYGG 2 cut(s) 57, 243
BtsCI GGATG 6 cut(s) 39, 164, 388, 406, 442, 460
BtsIMutI CAGTG 1 cut(s) 516
Cac8I GCNNGC 2 cut(s) 109, 535
Csp6I GTAC 2 cut(s) 124, 450
CviAII CATG 2 cut(s) 319, 361
CviQI GTAC 2 cut(s) 124, 450
DdeI CTNAG 2 cut(s) 183, 563
Eam1104I CTCTTC 3 cut(s) 33, 50, 244
EarI CTCTTC 3 cut(s) 33, 50, 244
Ecl136II GAGCTC 2 cut(s) 8, 340
Eco24I GRGCYC 2 cut(s) 10, 342
Eco53kI GAGCTC 2 cut(s) 8, 340
EcoICRI GAGCTC 2 cut(s) 8, 340
EcoT38I GRGCYC 2 cut(s) 10, 342
FaeI CATG 2 cut(s) 322, 364
FaiI YATR 5 cut(s) 201, 311, 320, 362, 407
FatI CATG 2 cut(s) 318, 360
FblI GTMKAC 1 cut(s) 333
Fnu4HI GCNGC 2 cut(s) 225, 483
FokI GGATG 6 cut(s) 26, 151, 395, 413, 449, 467
FriOI GRGCYC 2 cut(s) 10, 342
Fsp4HI GCNGC 2 cut(s) 225, 483
FspBI CTAG 3 cut(s) 525, 534, 571
GluI GCNGC 2 cut(s) 225, 483
HaeIII GGCC 1 cut(s) 290
HapII CCGG 1 cut(s) 291
Hin1II CATG 2 cut(s) 322, 364
HincII GTYRAC 1 cut(s) 334
HindII GTYRAC 1 cut(s) 334
HinfI GANTC 3 cut(s) 145, 326, 459
HpaII CCGG 1 cut(s) 291
HphI GGTGA 2 cut(s) 364, 499
Hpy166II GTNNAC 1 cut(s) 334
Hpy188I TCNGA 1 cut(s) 186
Hpy188III TCNNGA 1 cut(s) 52
Hpy8I GTNNAC 1 cut(s) 334
Hpy99I CGWCG 2 cut(s) 335, 338
HpyAV CCTTC 1 cut(s) 466
HpyCH4III ACNGT 2 cut(s) 247, 520
HpyCH4IV ACGT 2 cut(s) 96, 122
HpyCH4V TGCA 3 cut(s) 111, 195, 309
HpyF10VI GCNNNNNNNGC 4 cut(s) 233, 356, 530, 539
HpyF3I CTNAG 2 cut(s) 183, 563
HpySE526I ACGT 2 cut(s) 96, 122
Hsp92II CATG 2 cut(s) 322, 364
LmnI GCTCC 2 cut(s) 5, 13
LpnPI CCDG 5 cut(s) 121, 141, 304, 332, 460
LweI GCATC 1 cut(s) 367
MaeI CTAG 3 cut(s) 525, 534, 571
MaeII ACGT 2 cut(s) 96, 122
MaeIII GTNAC 3 cut(s) 211, 352, 566
MboII GAAGA 7 cut(s) 20, 37, 60, 63, 231, 277, 502
MhlI GDGCHC 2 cut(s) 10, 342
MluCI AATT 1 cut(s) 367
MlyI GAGTC 2 cut(s) 139, 320
MmeI TCCRAC 1 cut(s) 412
MseI TTAA 1 cut(s) 501
MslI CAYNNNNRTG 1 cut(s) 399
MspI CCGG 1 cut(s) 291
MspR9I CCNGG 1 cut(s) 292
Mva1269I GAATGC 1 cut(s) 510
MvnI CGCG 1 cut(s) 227
MwoI GCNNNNNNNGC 4 cut(s) 233, 356, 530, 539
NciI CCSGG 1 cut(s) 292
NheI GCTAGC 1 cut(s) 533
NlaIII CATG 2 cut(s) 322, 364
NmuCI GTSAC 1 cut(s) 352
PcsI WCGNNNNNNNCGW 1 cut(s) 165
PctI GAATGC 1 cut(s) 510
PfeI GAWTC 1 cut(s) 459
PflFI GACNNNGTC 1 cut(s) 329
PkrI GCNGC 2 cut(s) 226, 484
PleI GAGTC 2 cut(s) 139, 320
PpsI GAGTC 2 cut(s) 139, 320
Ppu21I YACGTR 1 cut(s) 123
PshBI ATTAAT 1 cut(s) 501
Psp124BI GAGCTC 2 cut(s) 10, 342
PsyI GACNNNGTC 1 cut(s) 329
RsaI GTAC 2 cut(s) 125, 451
RsaNI GTAC 2 cut(s) 124, 450
RseI CAYNNNNRTG 1 cut(s) 399
SacI GAGCTC 2 cut(s) 10, 342
SalI GTCGAC 1 cut(s) 332
SaqAI TTAA 1 cut(s) 501
SatI GCNGC 2 cut(s) 225, 483
SchI GAGTC 2 cut(s) 139, 320
ScrFI CCNGG 1 cut(s) 292
SduI GDGCHC 2 cut(s) 10, 342
SfaNI GCATC 1 cut(s) 367
SgrDI CGTCGACG 1 cut(s) 332
SmiMI CAYNNNNRTG 1 cut(s) 399
Sse9I AATT 1 cut(s) 367
SsiI CCGC 5 cut(s) 225, 350, 380, 482, 485
SspMI CTAG 3 cut(s) 525, 534, 571
SstI GAGCTC 2 cut(s) 10, 342
StyD4I CCNGG 1 cut(s) 290
TaaI ACNGT 2 cut(s) 247, 520
TaiI ACGT 2 cut(s) 99, 125
TaqI TCGA 4 cut(s) 74, 251, 333, 462
TasI AATT 1 cut(s) 367
TauI GCSGC 2 cut(s) 227, 485
TfiI GAWTC 1 cut(s) 459
Tru1I TTAA 1 cut(s) 501
Tru9I TTAA 1 cut(s) 501
TscAI CASTG 1 cut(s) 523
TseFI GTSAC 1 cut(s) 352
Tsp45I GTSAC 1 cut(s) 352
TspDTI ATGAA 4 cut(s) 28, 37, 377, 383
TspGWI ACGGA 5 cut(s) 157, 199, 319, 449, 467
TspRI CASTG 1 cut(s) 523
Tth111I GACNNNGTC 1 cut(s) 329
VspI ATTAAT 1 cut(s) 501
XapI RAATTY 1 cut(s) 367
XcmI CCANNNNNNNNNTGG 1 cut(s) 367
XmiI GTMKAC 1 cut(s) 333
XspI CTAG 3 cut(s) 525, 534, 571
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.