Rw1G035110

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0005396
Physical Location & Seq
Forward (+)
62949230 .. 62949814
585 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G035110.1

Sequence Viewer

Length: 573 bp
ATGGGAACTCCCACATTTCTCTCCACTCTTCTCCTATTCATCCTCTTCATCAACACGACCACGGCTACTTCTTCGAGCCAAACCTACAAAACCTACGTCAAAACAGCCTGCAACTCCACCCCGTACCCAGAAATTTGCTACAAGGCTCTCTCCTCCTACGCCAGATCCGTCAAATCAAACCCTCAGAAGCTCTGCAACTATGCCCTCTCCGTAACCCTAAAAGCCGCGAAAAAAGCCTCTTCCACGGTGTCGAAGCTGTCCAATAAGAAAGGATTGACGAAAGCAGAGGCCGGGGTAGTGGAGGATTGCATAGAGAACATTGAGGATTCCGTCGATGAGCTCAAACAGGCGGTGACTGCCATGAAAAATTTGGGAGATGCGGATGTCCAACTTCATTTGGATGACATAAAGACTTGGGTTAGCGCTTCGCTGACGGATGAGGACACCTGTACGGATGGATTAGATGAGCAGAAGGTAAGTGCGGCGGTGAAGAACACCATTAATAAGAGCATTCTCACTGTTGCTAGATTGGCTAGCAATGCTTTATCTCTCATCAACAACCTCAGTTACTAG

Protein Analysis

190

Amino Acids

20.49

Weight (kDa)

7.55

Isoelectric Point (pI)

36.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 32 - 184 1.1e-37 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 227
AciI CCGC 5 cut(s) 225, 350, 380, 482, 485
AclWI GGATC 1 cut(s) 159
AcsI RAATTY 2 cut(s) 132, 367
AfaI GTAC 2 cut(s) 125, 451
AfeI AGCGCT 1 cut(s) 424
AluBI AGCT 3 cut(s) 190, 256, 340
AluI AGCT 3 cut(s) 190, 256, 340
Alw21I GWGCWC 1 cut(s) 342
AlwI GGATC 1 cut(s) 159
Aor51HI AGCGCT 1 cut(s) 424
AoxI GGCC 1 cut(s) 288
ApoI RAATTY 2 cut(s) 132, 367
ArsI GACNNNNNNTTYG 2 cut(s) 81, 113
AseI ATTAAT 1 cut(s) 501
AspLEI GCGC 1 cut(s) 425
AsuC2I CCSGG 1 cut(s) 292
AsuHPI GGTGA 2 cut(s) 364, 499
AsuNHI GCTAGC 1 cut(s) 533
BanII GRGCYC 1 cut(s) 342
Bbv12I GWGCWC 1 cut(s) 342
BccI CCATC 1 cut(s) 449
BceAI ACGGC 1 cut(s) 78
BcnI CCSGG 1 cut(s) 292
BfaI CTAG 3 cut(s) 525, 534, 571
BfoI RGCGCY 1 cut(s) 426
BisI GCNGC 2 cut(s) 225, 483
BlsI GCNGC 2 cut(s) 226, 484
Bme1390I CCNGG 1 cut(s) 292
BmrFI CCNGG 1 cut(s) 292
BmsI GCATC 1 cut(s) 367
BmtI GCTAGC 1 cut(s) 537
BpuMI CCSGG 1 cut(s) 292
BsaJI CCNNGG 3 cut(s) 60, 243, 291
Bse3DI GCAATG 1 cut(s) 544
BseDI CCNNGG 3 cut(s) 60, 243, 291
BseGI GGATG 5 cut(s) 39, 388, 406, 442, 460
BseMI GCAATG 1 cut(s) 544
BseMII CTCAG 1 cut(s) 197
BseRI GAGGAG 1 cut(s) 142
Bsh1236I CGCG 1 cut(s) 227
BshFI GGCC 1 cut(s) 290
BsiHKAI GWGCWC 1 cut(s) 342
BsiSI CCGG 1 cut(s) 291
BsmI GAATGC 1 cut(s) 510
BsnI GGCC 1 cut(s) 290
Bsp1286I GDGCHC 1 cut(s) 342
Bsp143I GATC 1 cut(s) 164
BspACI CCGC 5 cut(s) 225, 350, 380, 482, 485
BspANI GGCC 1 cut(s) 290
BspCNI CTCAG 1 cut(s) 196
BspFNI CGCG 1 cut(s) 227
BspOI GCTAGC 1 cut(s) 537
BspPI GGATC 1 cut(s) 159
BsrDI GCAATG 1 cut(s) 544
BssECI CCNNGG 3 cut(s) 60, 243, 291
BssMI GATC 1 cut(s) 164
Bst4CI ACNGT 2 cut(s) 247, 520
Bst6I CTCTTC 3 cut(s) 33, 50, 244
BstC8I GCNNGC 2 cut(s) 109, 535
BstDEI CTNAG 2 cut(s) 183, 563
BstDSI CCRYGG 2 cut(s) 60, 243
BstF5I GGATG 5 cut(s) 39, 388, 406, 442, 460
BstFNI CGCG 1 cut(s) 227
BstH2I RGCGCY 1 cut(s) 426
BstHHI GCGC 1 cut(s) 425
BstKTI GATC 1 cut(s) 167
BstMBI GATC 1 cut(s) 164
BstMWI GCNNNNNNNGC 4 cut(s) 233, 356, 530, 539
BstSCI CCNGG 1 cut(s) 290
BstUI CGCG 1 cut(s) 227
BstX2I RGATCY 1 cut(s) 164
BstYI RGATCY 1 cut(s) 164
BsuRI GGCC 1 cut(s) 290
BtgI CCRYGG 2 cut(s) 60, 243
BtsCI GGATG 5 cut(s) 39, 388, 406, 442, 460
BtsIMutI CAGTG 1 cut(s) 516
Cac8I GCNNGC 2 cut(s) 109, 535
CfoI GCGC 1 cut(s) 425
Csp6I GTAC 2 cut(s) 124, 450
CviAII CATG 1 cut(s) 361
CviQI GTAC 2 cut(s) 124, 450
DdeI CTNAG 2 cut(s) 183, 563
DpnI GATC 1 cut(s) 166
DpnII GATC 1 cut(s) 164
Eam1104I CTCTTC 3 cut(s) 33, 50, 244
EarI CTCTTC 3 cut(s) 33, 50, 244
Ecl136II GAGCTC 1 cut(s) 340
Eco24I GRGCYC 1 cut(s) 342
Eco47III AGCGCT 1 cut(s) 424
Eco53kI GAGCTC 1 cut(s) 340
EcoICRI GAGCTC 1 cut(s) 340
EcoT38I GRGCYC 1 cut(s) 342
FaeI CATG 1 cut(s) 364
FaiI YATR 4 cut(s) 201, 311, 362, 407
FatI CATG 1 cut(s) 360
Fnu4HI GCNGC 2 cut(s) 225, 483
FokI GGATG 5 cut(s) 26, 395, 413, 449, 467
FriOI GRGCYC 1 cut(s) 342
Fsp4HI GCNGC 2 cut(s) 225, 483
FspBI CTAG 3 cut(s) 525, 534, 571
GlaI GCGC 1 cut(s) 424
GluI GCNGC 2 cut(s) 225, 483
HaeII RGCGCY 1 cut(s) 426
HaeIII GGCC 1 cut(s) 290
HapII CCGG 1 cut(s) 291
HhaI GCGC 1 cut(s) 425
Hin1II CATG 1 cut(s) 364
Hin6I GCGC 1 cut(s) 423
HinP1I GCGC 1 cut(s) 423
HinfI GANTC 1 cut(s) 326
HpaII CCGG 1 cut(s) 291
HphI GGTGA 2 cut(s) 364, 499
Hpy188I TCNGA 1 cut(s) 186
Hpy99I CGWCG 1 cut(s) 335
HpyAV CCTTC 1 cut(s) 466
HpyCH4III ACNGT 2 cut(s) 247, 520
HpyCH4IV ACGT 1 cut(s) 96
HpyCH4V TGCA 3 cut(s) 111, 195, 309
HpyF10VI GCNNNNNNNGC 4 cut(s) 233, 356, 530, 539
HpyF3I CTNAG 2 cut(s) 183, 563
HpySE526I ACGT 1 cut(s) 96
Hsp92II CATG 1 cut(s) 364
HspAI GCGC 1 cut(s) 423
Kzo9I GATC 1 cut(s) 164
LpnPI CCDG 6 cut(s) 121, 141, 175, 304, 332, 460
LweI GCATC 1 cut(s) 367
MaeI CTAG 3 cut(s) 525, 534, 571
MaeII ACGT 1 cut(s) 96
MaeIII GTNAC 3 cut(s) 211, 352, 566
MalI GATC 1 cut(s) 166
MboI GATC 1 cut(s) 164
MboII GAAGA 5 cut(s) 20, 37, 63, 231, 502
MflI RGATCY 1 cut(s) 164
MhlI GDGCHC 1 cut(s) 342
MluCI AATT 2 cut(s) 132, 367
MmeI TCCRAC 1 cut(s) 412
MseI TTAA 1 cut(s) 501
MslI CAYNNNNRTG 1 cut(s) 399
MspI CCGG 1 cut(s) 291
MspR9I CCNGG 1 cut(s) 292
Mva1269I GAATGC 1 cut(s) 510
MvnI CGCG 1 cut(s) 227
MwoI GCNNNNNNNGC 4 cut(s) 233, 356, 530, 539
NciI CCSGG 1 cut(s) 292
NdeII GATC 1 cut(s) 164
NheI GCTAGC 1 cut(s) 533
NlaIII CATG 1 cut(s) 364
NmuCI GTSAC 1 cut(s) 352
PcsI WCGNNNNNNNCGW 1 cut(s) 165
PctI GAATGC 1 cut(s) 510
PfeI GAWTC 1 cut(s) 326
PkrI GCNGC 2 cut(s) 226, 484
PshBI ATTAAT 1 cut(s) 501
Psp124BI GAGCTC 1 cut(s) 342
PsuI RGATCY 1 cut(s) 164
RsaI GTAC 2 cut(s) 125, 451
RsaNI GTAC 2 cut(s) 124, 450
RseI CAYNNNNRTG 1 cut(s) 399
SacI GAGCTC 1 cut(s) 342
SaqAI TTAA 1 cut(s) 501
SatI GCNGC 2 cut(s) 225, 483
Sau3AI GATC 1 cut(s) 164
ScrFI CCNGG 1 cut(s) 292
SduI GDGCHC 1 cut(s) 342
SetI ASST 9 cut(s) 86, 95, 99, 192, 258, 342, 449, 477, 564
SfaNI GCATC 1 cut(s) 367
SmiMI CAYNNNNRTG 1 cut(s) 399
Sse9I AATT 2 cut(s) 132, 367
SsiI CCGC 5 cut(s) 225, 350, 380, 482, 485
SspMI CTAG 3 cut(s) 525, 534, 571
SstI GAGCTC 1 cut(s) 342
StyD4I CCNGG 1 cut(s) 290
TaaI ACNGT 2 cut(s) 247, 520
TaiI ACGT 1 cut(s) 99
TaqI TCGA 3 cut(s) 74, 251, 333
TasI AATT 2 cut(s) 132, 367
TauI GCSGC 2 cut(s) 227, 485
TfiI GAWTC 1 cut(s) 326
Tru1I TTAA 1 cut(s) 501
Tru9I TTAA 1 cut(s) 501
TscAI CASTG 1 cut(s) 523
TseFI GTSAC 1 cut(s) 352
Tsp45I GTSAC 1 cut(s) 352
TspDTI ATGAA 4 cut(s) 28, 37, 377, 383
TspGWI ACGGA 5 cut(s) 157, 199, 319, 449, 467
TspRI CASTG 1 cut(s) 523
VspI ATTAAT 1 cut(s) 501
XapI RAATTY 2 cut(s) 132, 367
XcmI CCANNNNNNNNNTGG 1 cut(s) 367
XspI CTAG 3 cut(s) 525, 534, 571
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.