MD07G1255600.v1.1

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0005396
Physical Location & Seq
Forward (+)
32274076 .. 32274291
216 bp
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UTR
Exon/CDS
Intron
MD07G1255600.v1.1.491

Sequence Viewer

Length: 216 bp
ATGAAGAATTTGGGGGTCAAGGGTGGGGACGTGCAAGCGCAATTAGATGATATAAAGACTTGGGTCAGTGCTGTCCTCACAGACGACGCTACTTGCACTGATGAATTCGATGATGTGAAGGTAAGCACGGCGATTAAGACCGCAATCAAGAACAGCATTGTGCCTGCTGCTAGGTTAGCTAGCAATGCTCTGTCGCTCATCGACAAACTCAGCTAG

Protein Analysis

72

Amino Acids

7.49

Weight (kDa)

4.72

Isoelectric Point (pI)

9.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 8 - 66 2.8e-12 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 141
AcsI RAATTY 2 cut(s) 7, 104
AjiI CACGTC 1 cut(s) 31
AluBI AGCT 2 cut(s) 179, 213
AluI AGCT 2 cut(s) 179, 213
ApeKI GCWGC 1 cut(s) 167
ApoI RAATTY 2 cut(s) 7, 104
AspLEI GCGC 1 cut(s) 40
AsuNHI GCTAGC 1 cut(s) 179
BbvI GCAGC 1 cut(s) 154
BceAI ACGGC 1 cut(s) 144
BfaI CTAG 3 cut(s) 171, 180, 214
BisI GCNGC 1 cut(s) 168
BlsI GCNGC 1 cut(s) 169
BmgBI CACGTC 1 cut(s) 31
BmtI GCTAGC 1 cut(s) 183
BoxI GACNNNNGTC 1 cut(s) 62
Bse3DI GCAATG 1 cut(s) 190
BseMI GCAATG 1 cut(s) 190
BseXI GCAGC 1 cut(s) 154
BslFI GGGAC 1 cut(s) 41
BsmFI GGGAC 1 cut(s) 41
BspACI CCGC 1 cut(s) 141
BspOI GCTAGC 1 cut(s) 183
BsrDI GCAATG 1 cut(s) 190
BstC8I GCNNGC 3 cut(s) 36, 165, 181
BstDEI CTNAG 1 cut(s) 209
BstHHI GCGC 1 cut(s) 40
BstMWI GCNNNNNNNGC 2 cut(s) 176, 185
BstPAI GACNNNNGTC 1 cut(s) 62
BstV1I GCAGC 1 cut(s) 154
BtrI CACGTC 1 cut(s) 31
BtsIMutI CAGTG 2 cut(s) 73, 96
Cac8I GCNNGC 3 cut(s) 36, 165, 181
CfoI GCGC 1 cut(s) 40
CseI GACGC 1 cut(s) 95
CviJI RGCY 2 cut(s) 179, 213
CviKI_1 RGCY 2 cut(s) 179, 213
DdeI CTNAG 1 cut(s) 209
EcoRI GAATTC 1 cut(s) 104
FaiI YATR 1 cut(s) 53
FaqI GGGAC 1 cut(s) 41
Fnu4HI GCNGC 1 cut(s) 168
Fsp4HI GCNGC 1 cut(s) 168
FspBI CTAG 3 cut(s) 171, 180, 214
GlaI GCGC 1 cut(s) 39
GluI GCNGC 1 cut(s) 168
HgaI GACGC 1 cut(s) 95
HhaI GCGC 1 cut(s) 40
Hin6I GCGC 1 cut(s) 38
HinP1I GCGC 1 cut(s) 38
Hpy188III TCNNGA 1 cut(s) 148
Hpy99I CGWCG 1 cut(s) 89
HpyAV CCTTC 1 cut(s) 112
HpyCH4IV ACGT 1 cut(s) 30
HpyCH4V TGCA 2 cut(s) 34, 96
HpyF10VI GCNNNNNNNGC 2 cut(s) 176, 185
HpyF3I CTNAG 1 cut(s) 209
HpySE526I ACGT 1 cut(s) 30
HspAI GCGC 1 cut(s) 38
LpnPI CCDG 1 cut(s) 177
Lsp1109I GCAGC 1 cut(s) 154
MaeI CTAG 3 cut(s) 171, 180, 214
MaeII ACGT 1 cut(s) 30
MboII GAAGA 1 cut(s) 16
MluCI AATT 3 cut(s) 7, 41, 104
MnlI CCTC 1 cut(s) 86
MseI TTAA 1 cut(s) 135
MwoI GCNNNNNNNGC 2 cut(s) 176, 185
NheI GCTAGC 1 cut(s) 179
PkrI GCNGC 1 cut(s) 169
PshAI GACNNNNGTC 1 cut(s) 62
SaqAI TTAA 1 cut(s) 135
SatI GCNGC 1 cut(s) 168
SetI ASST 5 cut(s) 33, 123, 176, 181, 215
Sse9I AATT 3 cut(s) 7, 41, 104
SsiI CCGC 1 cut(s) 141
SspMI CTAG 3 cut(s) 171, 180, 214
TaiI ACGT 1 cut(s) 33
TaqI TCGA 2 cut(s) 108, 201
TasI AATT 3 cut(s) 7, 41, 104
Tru1I TTAA 1 cut(s) 135
Tru9I TTAA 1 cut(s) 135
TscAI CASTG 2 cut(s) 73, 103
TseI GCWGC 1 cut(s) 167
TspDTI ATGAA 2 cut(s) 17, 117
TspRI CASTG 2 cut(s) 73, 103
XapI RAATTY 2 cut(s) 7, 104
XspI CTAG 3 cut(s) 171, 180, 214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.