Rh1CG373600

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0005396
Physical Location & Seq
Forward (+)
64289579 .. 64290975
1397 bp
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UTR
Exon/CDS
Intron
Rh1CG373600.1

Sequence Viewer

Length: 660 bp
ATGCCTATAAATTGGTGTTGGCCTCTCACACAGTCACCCATCAATCCGAGAAAAACAAAAATGGGAGCTCAAACTCTCATATCTCATGTCACTCTTCTCCCATTTATCATTATCATCATCATGATAAACATGCACACAACCATGGCAGCCTCCTCTTCAACCCCAGAACTTCACAACTCCTATCTCCTAAGAGCTTGCAATTCAACCACATACCCACAAATTTGCTACAAGTCCCTCTTCTCCTACGCCTCCACAATCAACTCAAACCCTCAGAAGCTCTGCAGCTACGCCCTCTCTGTCGCCCTTAAAGCCGCCCGCAACGCCTCCTCCACCGTGACGAAGTTGTCCAAGAGGAATGGATTGACACACTCAGAGGCTTTGGTCGTCAAGGATTGCATAGAGAATATCAGGGACTCCATTAACGAGCTCAAGCAGTCCGTGAGTGCCATGAGCAACTTGGGAGGTGCCAATATGGAATACCAGTTGGGTGACATAAAGACTTGGGTCAGCGCTGCCCTAACAGACGATGATACTTGTATGGAAGGGTTTGATGAGGTGAAGGTGAGCTTGGATGTGAAGAACGCCATCAGTAAGAGCATCATCAATATTGCTAGATTGGCAAGTAATGCCCTGTCTCTCATCAACAGCCTCAATTATTAG

Protein Analysis

219

Amino Acids

24.01

Weight (kDa)

8.71

Isoelectric Point (pI)

44.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 64 - 213 1.1e-34 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 343
AccB1I GGYRCC 1 cut(s) 464
AciI CCGC 2 cut(s) 312, 316
AcsI RAATTY 1 cut(s) 219
AfeI AGCGCT 1 cut(s) 511
AgsI TTSAA 2 cut(s) 159, 204
AjuI GAANNNNNNNTTGG 2 cut(s) 551, 583
AluBI AGCT 6 cut(s) 68, 194, 277, 285, 427, 567
AluI AGCT 6 cut(s) 68, 194, 277, 285, 427, 567
Alw21I GWGCWC 2 cut(s) 70, 429
Alw26I GTCTC 1 cut(s) 639
Aor51HI AGCGCT 1 cut(s) 511
AoxI GGCC 1 cut(s) 20
ApeKI GCWGC 3 cut(s) 146, 282, 512
ApoI RAATTY 1 cut(s) 219
AspLEI GCGC 1 cut(s) 512
AsuHPI GGTGA 4 cut(s) 27, 500, 568, 574
BanI GGYRCC 1 cut(s) 464
BanII GRGCYC 2 cut(s) 70, 429
Bbv12I GWGCWC 2 cut(s) 70, 429
BbvI GCAGC 3 cut(s) 158, 294, 499
BccI CCATC 2 cut(s) 47, 593
BcoDI GTCTC 1 cut(s) 639
BfaI CTAG 1 cut(s) 612
BfmI CTRYAG 1 cut(s) 280
BfoI RGCGCY 1 cut(s) 513
BisI GCNGC 4 cut(s) 147, 283, 312, 513
BlsI GCNGC 4 cut(s) 148, 284, 313, 514
BmiI GGNNCC 1 cut(s) 466
BmsI GCATC 1 cut(s) 606
BoxI GACNNNNGTC 1 cut(s) 503
BpuEI CTTGAG 1 cut(s) 413
BsaJI CCNNGG 1 cut(s) 141
BsaXI ACNNNNNCTCC 2 cut(s) 311, 341
Bse1I ACTGG 1 cut(s) 481
BseDI CCNNGG 1 cut(s) 141
BseGI GGATG 1 cut(s) 577
BseMII CTCAG 2 cut(s) 284, 384
BseNI ACTGG 1 cut(s) 481
BseRI GAGGAG 2 cut(s) 142, 316
BseXI GCAGC 3 cut(s) 158, 294, 499
BshFI GGCC 1 cut(s) 22
BshNI GGYRCC 1 cut(s) 464
BsiHKAI GWGCWC 2 cut(s) 70, 429
BslFI GGGAC 2 cut(s) 217, 425
BsmAI GTCTC 1 cut(s) 639
BsmFI GGGAC 2 cut(s) 217, 425
BsnI GGCC 1 cut(s) 22
Bsp1286I GDGCHC 2 cut(s) 70, 429
Bsp19I CCATGG 1 cut(s) 141
BspACI CCGC 2 cut(s) 312, 316
BspANI GGCC 1 cut(s) 22
BspCNI CTCAG 2 cut(s) 283, 383
BspHI TCATGA 1 cut(s) 120
BspLI GGNNCC 1 cut(s) 466
BspMAI CTGCAG 1 cut(s) 284
BspT107I GGYRCC 1 cut(s) 464
BsrI ACTGG 1 cut(s) 481
BssECI CCNNGG 1 cut(s) 141
BssT1I CCWWGG 1 cut(s) 141
Bst4CI ACNGT 2 cut(s) 33, 334
Bst6I CTCTTC 3 cut(s) 99, 160, 242
BstAPI GCANNNNNTGC 1 cut(s) 626
BstC8I GCNNGC 2 cut(s) 196, 316
BstDEI CTNAG 3 cut(s) 188, 270, 370
BstDSI CCRYGG 1 cut(s) 141
BstF5I GGATG 1 cut(s) 577
BstH2I RGCGCY 1 cut(s) 513
BstHHI GCGC 1 cut(s) 512
BstMAI GTCTC 1 cut(s) 639
BstMWI GCNNNNNNNGC 4 cut(s) 308, 320, 617, 626
BstNSI RCATGY 1 cut(s) 133
BstPAI GACNNNNGTC 1 cut(s) 503
BstSFI CTRYAG 1 cut(s) 280
BstV1I GCAGC 3 cut(s) 158, 294, 499
BsuRI GGCC 1 cut(s) 22
BtgI CCRYGG 1 cut(s) 141
BtsCI GGATG 1 cut(s) 577
Cac8I GCNNGC 2 cut(s) 196, 316
CciI TCATGA 1 cut(s) 120
CfoI GCGC 1 cut(s) 512
CviAII CATG 5 cut(s) 86, 121, 130, 142, 448
DdeI CTNAG 3 cut(s) 188, 270, 370
DrdI GACNNNNNNGTC 1 cut(s) 343
DseDI GACNNNNNNGTC 1 cut(s) 343
Eam1104I CTCTTC 3 cut(s) 99, 160, 242
EarI CTCTTC 3 cut(s) 99, 160, 242
Ecl136II GAGCTC 2 cut(s) 68, 427
Eco130I CCWWGG 1 cut(s) 141
Eco24I GRGCYC 2 cut(s) 70, 429
Eco47III AGCGCT 1 cut(s) 511
Eco53kI GAGCTC 2 cut(s) 68, 427
EcoICRI GAGCTC 2 cut(s) 68, 427
EcoT14I CCWWGG 1 cut(s) 141
EcoT38I GRGCYC 2 cut(s) 70, 429
ErhI CCWWGG 1 cut(s) 141
FaeI CATG 5 cut(s) 89, 124, 133, 145, 451
FalI AAGNNNNNCTT 4 cut(s) 221, 253, 551, 583
FaqI GGGAC 2 cut(s) 217, 425
FatI CATG 5 cut(s) 85, 120, 129, 141, 447
FauI CCCGC 1 cut(s) 323
Fnu4HI GCNGC 4 cut(s) 147, 283, 312, 513
FokI GGATG 1 cut(s) 584
FriOI GRGCYC 2 cut(s) 70, 429
Fsp4HI GCNGC 4 cut(s) 147, 283, 312, 513
FspBI CTAG 1 cut(s) 612
GlaI GCGC 1 cut(s) 511
GluI GCNGC 4 cut(s) 147, 283, 312, 513
HaeII RGCGCY 1 cut(s) 513
HaeIII GGCC 1 cut(s) 22
HhaI GCGC 1 cut(s) 512
Hin1II CATG 5 cut(s) 89, 124, 133, 145, 451
Hin6I GCGC 1 cut(s) 510
HinP1I GCGC 1 cut(s) 510
HinfI GANTC 1 cut(s) 413
HphI GGTGA 4 cut(s) 27, 500, 568, 574
Hpy188I TCNGA 3 cut(s) 48, 273, 373
Hpy188III TCNNGA 1 cut(s) 121
HpyAV CCTTC 2 cut(s) 536, 553
HpyCH4III ACNGT 2 cut(s) 33, 334
HpyCH4V TGCA 4 cut(s) 133, 198, 282, 396
HpyF10VI GCNNNNNNNGC 4 cut(s) 308, 320, 617, 626
HpyF3I CTNAG 3 cut(s) 188, 270, 370
Hsp92II CATG 5 cut(s) 89, 124, 133, 145, 451
HspAI GCGC 1 cut(s) 510
LmnI GCTCC 1 cut(s) 65
LpnPI CCDG 4 cut(s) 177, 394, 494, 644
Lsp1109I GCAGC 3 cut(s) 158, 294, 499
LweI GCATC 1 cut(s) 606
MaeI CTAG 1 cut(s) 612
MaeIII GTNAC 4 cut(s) 33, 88, 334, 488
MboII GAAGA 4 cut(s) 86, 147, 229, 589
MhlI GDGCHC 2 cut(s) 70, 429
MluCI AATT 4 cut(s) 10, 199, 219, 652
MlyI GAGTC 1 cut(s) 407
MseI TTAA 2 cut(s) 306, 420
MslI CAYNNNNRTG 2 cut(s) 119, 140
MwoI GCNNNNNNNGC 4 cut(s) 308, 320, 617, 626
NcoI CCATGG 1 cut(s) 141
NlaIII CATG 5 cut(s) 89, 124, 133, 145, 451
NlaIV GGNNCC 1 cut(s) 466
NmuCI GTSAC 4 cut(s) 33, 88, 334, 488
NspI RCATGY 1 cut(s) 133
PagI TCATGA 1 cut(s) 120
PkrI GCNGC 4 cut(s) 148, 284, 313, 514
PleI GAGTC 1 cut(s) 407
PpsI GAGTC 1 cut(s) 407
PshAI GACNNNNGTC 1 cut(s) 503
Psp124BI GAGCTC 2 cut(s) 70, 429
PspN4I GGNNCC 1 cut(s) 466
PstI CTGCAG 1 cut(s) 284
RseI CAYNNNNRTG 2 cut(s) 119, 140
SacI GAGCTC 2 cut(s) 70, 429
SaqAI TTAA 2 cut(s) 306, 420
SatI GCNGC 4 cut(s) 147, 283, 312, 513
SchI GAGTC 1 cut(s) 407
SduI GDGCHC 2 cut(s) 70, 429
SetI ASST 9 cut(s) 70, 196, 279, 287, 429, 466, 558, 564, 569
SfaNI GCATC 1 cut(s) 606
SfcI CTRYAG 1 cut(s) 280
SmiMI CAYNNNNRTG 2 cut(s) 119, 140
SmlI CTYRAG 1 cut(s) 428
SmoI CTYRAG 1 cut(s) 428
Sse9I AATT 4 cut(s) 10, 199, 219, 652
SsiI CCGC 2 cut(s) 312, 316
SspI AATATT 1 cut(s) 607
SspMI CTAG 1 cut(s) 612
SstI GAGCTC 2 cut(s) 70, 429
StyI CCWWGG 1 cut(s) 141
TaaI ACNGT 2 cut(s) 33, 334
TasI AATT 4 cut(s) 10, 199, 219, 652
TauI GCSGC 1 cut(s) 314
Tru1I TTAA 2 cut(s) 306, 420
Tru9I TTAA 2 cut(s) 306, 420
TseFI GTSAC 4 cut(s) 33, 88, 334, 488
TseI GCWGC 3 cut(s) 146, 282, 512
Tsp45I GTSAC 4 cut(s) 33, 88, 334, 488
TspGWI ACGGA 1 cut(s) 427
XapI RAATTY 1 cut(s) 219
XceI RCATGY 1 cut(s) 133
XcmI CCANNNNNNNNNTGG 1 cut(s) 454
XspI CTAG 1 cut(s) 612
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.