RchiOBHm_Chr1g0374711

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0005396
Physical Location & Seq
Forward (+)
62440786 .. 62441684
899 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ59850

Sequence Viewer

Length: 723 bp
ATGTGCCATCTCGTTTATTGCTTATATACTATTGAAAGAACAGCGCGCTCTCTCTCTATCTTTTTTTTGTTTTTGGCTAAAAATAATGTATTTGAGATTTGGACTAGTTCATGGTGTAAAAATTTAGACTTCATATATACTTTAATACTTCAAATGCCTATAAATTGGTGTTGGCCTCCTCTCACACAGTCACCCATCAATCCGAGAAAAACAAAAATGGGAGCTCAAACTCTCATATCTCATGTCACTCTTCTCCCATTTATCATTATCATCATCATGATAAACATGCACACAACCATGGCAGCCTCCTCTTCAACCCCAGAACCTCACAACTCCTATCTCCTAAGAGCTTGCAATTCAACCACATACCCACAAATTTGCTACAAGTCCCTCTTCTCCTACGCCTCCACAATCAACTCAAACCCTCAGAAGCTCTGCAGCTTCGCCCTCTCTGTCGCCCTTAAAGCCGCCCGCAACGCCTCCTCCACCGTGACGAAGCTGTCCAAGAGGAATGGATTGACACACTCAGAGGCTTTGGTCGTCAAGGATTGCATAGAGAATATCAGGGACTCCATTGACGAGCTCAAGCAGTCCGTGAGTGCCATGAGCAACTTGGGAGGTGCCAATATGGAATACCAGTTGGGTGACATAAAGACTTGGGTCAGCGCTGCCCTAACAGACGATGATACTTGTATGGAAGGGTTTGATGAGGTGAAGGGCTAG

Protein Analysis

240

Amino Acids

26.89

Weight (kDa)

8.04

Isoelectric Point (pI)

48.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 116 - 238 6e-31 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 499
AccB1I GGYRCC 1 cut(s) 620
AccII CGCG 1 cut(s) 46
AciI CCGC 2 cut(s) 468, 472
AcsI RAATTY 2 cut(s) 121, 375
AfeI AGCGCT 1 cut(s) 667
AgsI TTSAA 4 cut(s) 35, 152, 315, 360
AhlI ACTAGT 1 cut(s) 104
AluBI AGCT 6 cut(s) 224, 350, 433, 441, 499, 583
AluI AGCT 6 cut(s) 224, 350, 433, 441, 499, 583
Alw21I GWGCWC 2 cut(s) 226, 585
Aor51HI AGCGCT 1 cut(s) 667
AoxI GGCC 1 cut(s) 173
ApeKI GCWGC 3 cut(s) 302, 438, 668
ApoI RAATTY 2 cut(s) 121, 375
AspLEI GCGC 3 cut(s) 46, 48, 668
AsuHPI GGTGA 2 cut(s) 183, 656
BanI GGYRCC 1 cut(s) 620
BanII GRGCYC 2 cut(s) 226, 585
Bbv12I GWGCWC 2 cut(s) 226, 585
BbvI GCAGC 3 cut(s) 314, 450, 655
BccI CCATC 2 cut(s) 15, 203
BcuI ACTAGT 1 cut(s) 104
BfaI CTAG 2 cut(s) 105, 721
BfmI CTRYAG 1 cut(s) 436
BfoI RGCGCY 1 cut(s) 669
BisI GCNGC 4 cut(s) 303, 439, 468, 669
BlsI GCNGC 4 cut(s) 304, 440, 469, 670
BmiI GGNNCC 1 cut(s) 622
BoxI GACNNNNGTC 1 cut(s) 659
BpuEI CTTGAG 1 cut(s) 569
BsaJI CCNNGG 1 cut(s) 297
BsaXI ACNNNNNCTCC 2 cut(s) 467, 497
Bse1I ACTGG 1 cut(s) 637
BseDI CCNNGG 1 cut(s) 297
BseMII CTCAG 2 cut(s) 440, 540
BseNI ACTGG 1 cut(s) 637
BsePI GCGCGC 1 cut(s) 44
BseRI GAGGAG 3 cut(s) 168, 298, 472
BseXI GCAGC 3 cut(s) 314, 450, 655
Bsh1236I CGCG 1 cut(s) 46
BshFI GGCC 1 cut(s) 175
BshNI GGYRCC 1 cut(s) 620
BsiHKAI GWGCWC 2 cut(s) 226, 585
BslFI GGGAC 2 cut(s) 373, 581
BsmFI GGGAC 2 cut(s) 373, 581
BsnI GGCC 1 cut(s) 175
Bsp1286I GDGCHC 2 cut(s) 226, 585
Bsp19I CCATGG 1 cut(s) 297
BspACI CCGC 2 cut(s) 468, 472
BspANI GGCC 1 cut(s) 175
BspCNI CTCAG 2 cut(s) 439, 539
BspFNI CGCG 1 cut(s) 46
BspHI TCATGA 1 cut(s) 276
BspLI GGNNCC 1 cut(s) 622
BspMAI CTGCAG 1 cut(s) 440
BspT107I GGYRCC 1 cut(s) 620
BsrI ACTGG 1 cut(s) 637
BssECI CCNNGG 1 cut(s) 297
BssHII GCGCGC 1 cut(s) 44
BssT1I CCWWGG 1 cut(s) 297
Bst4CI ACNGT 2 cut(s) 189, 490
Bst6I CTCTTC 3 cut(s) 255, 316, 398
BstC8I GCNNGC 3 cut(s) 46, 352, 472
BstDEI CTNAG 3 cut(s) 344, 426, 526
BstDSI CCRYGG 1 cut(s) 297
BstFNI CGCG 1 cut(s) 46
BstH2I RGCGCY 1 cut(s) 669
BstHHI GCGC 3 cut(s) 46, 48, 668
BstMWI GCNNNNNNNGC 2 cut(s) 464, 476
BstNSI RCATGY 1 cut(s) 289
BstPAI GACNNNNGTC 1 cut(s) 659
BstSFI CTRYAG 1 cut(s) 436
BstUI CGCG 1 cut(s) 46
BstV1I GCAGC 3 cut(s) 314, 450, 655
BsuRI GGCC 1 cut(s) 175
BtgI CCRYGG 1 cut(s) 297
Cac8I GCNNGC 3 cut(s) 46, 352, 472
CciI TCATGA 1 cut(s) 276
CfoI GCGC 3 cut(s) 46, 48, 668
CviAII CATG 6 cut(s) 111, 242, 277, 286, 298, 604
DdeI CTNAG 3 cut(s) 344, 426, 526
DrdI GACNNNNNNGTC 1 cut(s) 499
DseDI GACNNNNNNGTC 1 cut(s) 499
Eam1104I CTCTTC 3 cut(s) 255, 316, 398
EarI CTCTTC 3 cut(s) 255, 316, 398
Ecl136II GAGCTC 2 cut(s) 224, 583
Eco130I CCWWGG 1 cut(s) 297
Eco24I GRGCYC 2 cut(s) 226, 585
Eco47III AGCGCT 1 cut(s) 667
Eco53kI GAGCTC 2 cut(s) 224, 583
EcoICRI GAGCTC 2 cut(s) 224, 583
EcoT14I CCWWGG 1 cut(s) 297
EcoT38I GRGCYC 2 cut(s) 226, 585
ErhI CCWWGG 1 cut(s) 297
FaeI CATG 6 cut(s) 114, 245, 280, 289, 301, 607
FalI AAGNNNNNCTT 2 cut(s) 377, 409
FaqI GGGAC 2 cut(s) 373, 581
FatI CATG 6 cut(s) 110, 241, 276, 285, 297, 603
FauI CCCGC 1 cut(s) 479
Fnu4HI GCNGC 4 cut(s) 303, 439, 468, 669
FriOI GRGCYC 2 cut(s) 226, 585
Fsp4HI GCNGC 4 cut(s) 303, 439, 468, 669
FspBI CTAG 2 cut(s) 105, 721
GlaI GCGC 3 cut(s) 45, 47, 667
GluI GCNGC 4 cut(s) 303, 439, 468, 669
HaeII RGCGCY 1 cut(s) 669
HaeIII GGCC 1 cut(s) 175
HhaI GCGC 3 cut(s) 46, 48, 668
Hin1II CATG 6 cut(s) 114, 245, 280, 289, 301, 607
Hin6I GCGC 3 cut(s) 44, 46, 666
HinP1I GCGC 3 cut(s) 44, 46, 666
HinfI GANTC 1 cut(s) 569
HphI GGTGA 2 cut(s) 183, 656
Hpy188I TCNGA 3 cut(s) 204, 429, 529
Hpy188III TCNNGA 1 cut(s) 277
HpyAV CCTTC 2 cut(s) 692, 709
HpyCH4III ACNGT 2 cut(s) 189, 490
HpyCH4V TGCA 4 cut(s) 289, 354, 438, 552
HpyF10VI GCNNNNNNNGC 2 cut(s) 464, 476
HpyF3I CTNAG 3 cut(s) 344, 426, 526
Hsp92II CATG 6 cut(s) 114, 245, 280, 289, 301, 607
HspAI GCGC 3 cut(s) 44, 46, 666
LmnI GCTCC 1 cut(s) 221
LpnPI CCDG 3 cut(s) 333, 550, 650
Lsp1109I GCAGC 3 cut(s) 314, 450, 655
MaeI CTAG 2 cut(s) 105, 721
MaeIII GTNAC 4 cut(s) 189, 244, 490, 644
MboII GAAGA 3 cut(s) 242, 303, 385
MhlI GDGCHC 2 cut(s) 226, 585
MluCI AATT 4 cut(s) 121, 163, 355, 375
MlyI GAGTC 1 cut(s) 563
MseI TTAA 2 cut(s) 143, 462
MslI CAYNNNNRTG 2 cut(s) 275, 296
MvnI CGCG 1 cut(s) 46
MwoI GCNNNNNNNGC 2 cut(s) 464, 476
NcoI CCATGG 1 cut(s) 297
NlaIII CATG 6 cut(s) 114, 245, 280, 289, 301, 607
NlaIV GGNNCC 1 cut(s) 622
NmuCI GTSAC 4 cut(s) 189, 244, 490, 644
NspI RCATGY 1 cut(s) 289
PagI TCATGA 1 cut(s) 276
PauI GCGCGC 1 cut(s) 44
PkrI GCNGC 4 cut(s) 304, 440, 469, 670
PleI GAGTC 1 cut(s) 563
PpsI GAGTC 1 cut(s) 563
PshAI GACNNNNGTC 1 cut(s) 659
Psp124BI GAGCTC 2 cut(s) 226, 585
PspN4I GGNNCC 1 cut(s) 622
PstI CTGCAG 1 cut(s) 440
PteI GCGCGC 1 cut(s) 44
RseI CAYNNNNRTG 2 cut(s) 275, 296
SacI GAGCTC 2 cut(s) 226, 585
SaqAI TTAA 2 cut(s) 143, 462
SatI GCNGC 4 cut(s) 303, 439, 468, 669
SchI GAGTC 1 cut(s) 563
SduI GDGCHC 2 cut(s) 226, 585
SetI ASST 9 cut(s) 226, 328, 352, 435, 443, 501, 585, 622, 714
SfcI CTRYAG 1 cut(s) 436
SmiMI CAYNNNNRTG 2 cut(s) 275, 296
SmlI CTYRAG 1 cut(s) 584
SmoI CTYRAG 1 cut(s) 584
SpeI ACTAGT 1 cut(s) 104
Sse9I AATT 4 cut(s) 121, 163, 355, 375
SsiI CCGC 2 cut(s) 468, 472
SspMI CTAG 2 cut(s) 105, 721
SstI GAGCTC 2 cut(s) 226, 585
StyI CCWWGG 1 cut(s) 297
TaaI ACNGT 2 cut(s) 189, 490
TasI AATT 4 cut(s) 121, 163, 355, 375
TauI GCSGC 1 cut(s) 470
Tru1I TTAA 2 cut(s) 143, 462
Tru9I TTAA 2 cut(s) 143, 462
TseFI GTSAC 4 cut(s) 189, 244, 490, 644
TseI GCWGC 3 cut(s) 302, 438, 668
Tsp45I GTSAC 4 cut(s) 189, 244, 490, 644
TspDTI ATGAA 2 cut(s) 99, 121
TspGWI ACGGA 1 cut(s) 583
XapI RAATTY 2 cut(s) 121, 375
XceI RCATGY 1 cut(s) 289
XcmI CCANNNNNNNNNTGG 1 cut(s) 610
XspI CTAG 2 cut(s) 105, 721
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.